CN105777247A - Preparation method of selenium-enriched earthworm organic fertilizer - Google Patents
Preparation method of selenium-enriched earthworm organic fertilizer Download PDFInfo
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- CN105777247A CN105777247A CN201610059486.5A CN201610059486A CN105777247A CN 105777247 A CN105777247 A CN 105777247A CN 201610059486 A CN201610059486 A CN 201610059486A CN 105777247 A CN105777247 A CN 105777247A
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- selenium
- wormcast
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- lumbricus
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0332—Earthworms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
Abstract
The invention discloses a preparation method of a selenium-enriched earthworm organic fertilizer. The method includes the following steps: in accordance with the mass ratio of 1:1, weighing fermented livestock manure and mushroom dregs, sieving, and mixing evenly to obtain a substrate for feeding earthworms; preparing a selenium-enriched feeding substrate and a normal feeding substrate; introducing the earthworms into the obtained selenium-enriched feeding substrate and the obtained normal feeding substrate, feeding, and separating the earthworms and wormcast; preparing an earthworm homogenate; carrying out enzymatic hydrolysis of the earthworm homogenate, to obtain a selenium-enriched amino acid nutritional original solution; preparing a wormcast extraction liquid; and mixing the wormcast extraction liquid and the selenium-enriched amino acid nutritional original solution, and sterilizing to obtain the fertilizer. The preparation method of the selenium-enriched earthworm organic fertilizer has the characteristics of low production cost and short production cycle; and the obtained product has the advantages of long storage time, complete earthworm decomposition, high organic selenium content and the like.
Description
Technical field
The present invention relates to agricultural organic fertilizer technical field, be more particularly to the manufacture method of a kind of selenium-enriched earthworm fertilizer.
Background technology
Selenium is that humans and animals bulk-growth grows required trace element, is also the beneficial element of plant growing.Selenium participates in multiple important functional enzyme in composition body, and maintenance health and normal physiological metabolism are had particularly important effect.China's Selenium in Soil maldistribution of the resources is weighed and there is large-area scarce selenium area, and this selemium nutrition directly resulted in the selenium source food that human body is main is under-supply, and then affects health.Additionally, since 20th century, inorganic fertilizer and pesticide a large amount of use cause create such as Soil structure destruction, soil property worsen, a series of great environmental problem and the agricultural problem such as the quality of agricultural product and safety reduction, the harm of human body is constantly increased, how to solve agricultural product selenium deficiency simultaneously and use the negative agricultural problem that inorganic fertilizer etc. brings to become vast researcher and planted a great problem that raiser faces.
Lumbricus contains the nutrient substance such as a large amount of protein, the aminoacid of abundant species, vitamin and mineral element, is that a kind of well amino acid foliage fertilizer makes material.Lumbricus Se accumulation amount is big, and by biotransformation, inorganic selenium can be converted into organic selenium, and transformation efficiency is high.Wormcast contains a certain amount of nitrogen, phosphorus, potassium, multiple mineral element and substantial amounts of profitable strain, is the organic fertilizer of a kind of high-quality.The fertilizer made currently with Lumbricus and wormcast is how fertile as solid-based, need to use with other fertilizer and Se content is relatively low, still can not meet the demand of plant selenium-rich, and some currently used fertilizer rich in selenium mostly are inorganic selenium fertilizer, chronic administration or use the security risk that improper existence is certain.
Summary of the invention
(1) to solve the technical problem that
The technical problem to be solved in the present invention is how to improve the Se content in fertilizer, and provides the manufacture method of selenium-enriched earthworm fertilizer.
(2) technical scheme
In order to solve above-mentioned technical problem, the invention provides the manufacture method of a kind of selenium-enriched earthworm fertilizer, the method comprises the steps:
Step one: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, crosses 3-5mm sieve respectively, is then sufficiently mixed uniformly, obtains raising the substrate of Lumbricus；
Step 2: preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, joins the sodium selenite aqueous solution of 0.5-1.0L in the substrate of the 12.5-25kg weight that step one obtains；In the another substrate that a certain amount of water is joined the other 12.5-25kg weight that step one obtains；Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust；
Step 3: introduce Lumbricus in the selenium-rich breeding dust obtained in step 2 and common breeding dust, raise at 22-28 DEG C of temperature, once identical with former substrate substrate is added respectively every 15-20 days, after earthworm breeding 40-50 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm, standby；
Step 4: selenium-enriched earthworm step 3 obtained is bowel relieving 20-26 hour under dark condition, then cleans with clear water, clean selenium-enriched earthworm tissue refiner is carried out homogenizing homogenate, obtains Lumbricus homogenate；
Step 5: Lumbricus homogenate step 4 obtained utilizes the multiple enzyme that Lumbricus self contains to carry out self-dissolving enzymolysis under 50-60 DEG C of condition, every stirring in 5-6 hour once, enzymolysis 20-26 hour, obtain selenium-rich amino acid nutrition stock solution；The present invention need not additional fermenting agent, cost is low, and efficiency is high, simple to operate.
Step 6: common wormcast step 3 obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5-6, it is sufficiently stirred at ambient temperature, it is filtered after stirring 3-5 hour, it is filtrated to get wormcast extracting solution, the selenium-rich amino acid nutrition stock solution that above-mentioned wormcast extracting solution obtains with step 5 is mixed by a certain percentage, by mixed liquor sterilizing 1.5-2.5 hour under 100 DEG C of conditions, then cooling, filtration, to obtain final product.Sterilizing is to prevent in later stage storage process owing to bacterial activity produces rotten, fouling phenomenon, extending fertilizer period of storage.
Preferably, described feces of livestock and poultry is cattle manure.
Preferably, described dreg is the residue of mushroom culture medium material, it is preferable that the residue of culture medium for golden mushroom material.
Preferably, described Lumbricus is girdled adult Eisenia foetida.Because the compatriot that grows up likes that victory earthworm bioaccumulation and the ability being converted into organic selenium are strong.
Preferably, in step 6, described wormcast extracting solution mixes according to volume ratio 1:0.8-1.2 with selenium-rich amino acid nutrition stock solution.
Preferably, described method comprises the steps:
Step one: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, crosses 4mm sieve respectively, is then sufficiently mixed uniformly, obtains raising the substrate of Lumbricus；
Step 2: preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, joins 0.8L sodium selenite aqueous solution in the substrate of the 20kg weight that step one obtains；In the another substrate that a certain amount of water is joined the other 20kg weight that step one obtains；Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust；
Step 3: introduce Lumbricus in the selenium-rich breeding dust obtained in step 2 and common breeding dust, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 18 days respectively, earthworm breeding is after 45 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm；
Step 4: selenium-enriched earthworm step 3 obtained is bowel relieving 24 hours under dark condition, then cleans with clear water, clean selenium-enriched earthworm tissue refiner is carried out homogenizing homogenate；
Step 5: Lumbricus homogenate step 4 obtained utilizes the multiple enzyme that Lumbricus self contains to carry out self-dissolving enzymolysis under 55 DEG C of conditions, every stirring in 5.5 hours once, enzymolysis 24 hours, obtain selenium-rich amino acid nutrition stock solution；
Step 6: common wormcast step 3 obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5.5, it is sufficiently stirred at ambient temperature, it is filtered after stirring 4 hours, it is filtrated to get wormcast extracting solution, the selenium-rich amino acid nutrition stock solution that above-mentioned wormcast extracting solution obtains with step 5 is mixed according to volume ratio 1:1, by mixed liquor sterilizing 2 hours under 100 DEG C of conditions, then cooling, filtration, to obtain final product.
Present invention also offers the selenium-enriched earthworm fertilizer that the manufacture method of described selenium-enriched earthworm fertilizer prepares, its total Se content is about 8-12mg/L, and wherein organic selenium content accounts for more than 96%.
Preferably, the pH value of described selenium-enriched earthworm fertilizer is 6.8, close to neutral.This is advantageously in plant stem-leaf absorbing fertilizer Middle nutrition element.
Selenium-enriched earthworm fertilizer of the present invention according to the nutritional need of Different Crop and fruit and vegerable, directly can use after diluting suitable multiple.
(3) beneficial effect
The present invention adopts fermentation cattle manure and dreg to be substrate, and artificially adds selenium (Na2SeO3) raise ripe compatriot and love victory Lumbricus, enrichment and to convert the efficiency of organic selenium high, and selenium-enriched earthworm is to make the material that selenium-rich amino acid is fertile fabulous.Furthermore, containing the trace element such as a large amount of nitrogen, phosphorus, potassium element and copper, ferrum, zinc in common wormcast, and amino acid classes and rich content, it is the SOLID ORGANIC fertilizer of a kind of ecological, environmental protective.Additionally, selenium main and protein bound in Lumbricus body, after enzymolysis, selenium mainly exists with the form of the organics such as selenomethionine in this selenium-enriched earthworm fertilizer, is conducive to the plant leaf blade of corn, vegetable, fruit tree etc. absorb and utilize.In selenium-enriched earthworm fertilizer of the present invention, inorganic selenium content is few, environment will not be had a negative impact, be a kind of environmentally friendly selenium-enriched earthworm fertilizer.Finally, this fertilizer Se content is abundant, amino acid content is high, Acidity of Aikalinity, close to neutral, advantageously absorbs in plant stem-leaf, has promotion crop and fruit and vegerable growth, sterilization, suppresses Metal uptake to accumulate, improve the yield and quality, improve the critical functions such as Se accumulation amount.
Detailed description of the invention
Below in conjunction with embodiment, embodiments of the present invention are described in further detail.Following example are used for illustrating the present invention, but can not be used for limiting the scope of the present invention.
The invention discloses a kind of fertilizer for improving the Se content of crops, vegetable and fruit, yield and quality, be mainly made up of organic selenium, earthworm-amino acid, wormcast extracting solution and various nutrient elements.The present invention is further illustrated below by manufacture method embodiment.
Embodiment 1
Make selenium-enriched earthworm fertilizer and include step: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, cross 4mm sieve respectively, be then sufficiently mixed uniformly, obtain raising the substrate of Lumbricus；Preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, is joined by 0.8L sodium selenite aqueous solution in the substrate of the 20kg weight obtained；In the another substrate that a certain amount of water is joined the other 20kg weight obtained；Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust；The selenium-rich breeding dust obtained and common breeding dust introduce Lumbricus, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 18 days respectively, earthworm breeding is after 45 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm；Selenium-enriched earthworm bowel relieving 24 hours under dark condition that will obtain, then clean with clear water, clean selenium-enriched earthworm tissue refiner are carried out homogenizing homogenate；The multiple enzyme that Lumbricus self contains is utilized to carry out self-dissolving enzymolysis under 55 DEG C of conditions the Lumbricus homogenate obtained, every stirring in 5.5 hours once, enzymolysis 24 hours, obtain selenium-rich amino acid nutrition stock solution；The common wormcast obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5.5, it is sufficiently stirred at ambient temperature, it is filtered after stirring 4 hours, it is filtrated to get wormcast extracting solution, wormcast extracting solution is mixed by 1:1 volume ratio with selenium-rich amino acid nutrition stock solution, by mixed liquor sterilizing 2 hours under 100 DEG C of conditions, then cooling, filtration, obtaining selenium-enriched earthworm fertilizer, its Se content is 10.62mg/L, and wherein organic selenium content is up to 97.32%.
Embodiment 2
Make selenium-enriched earthworm fertilizer and include step: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, cross 3mm sieve respectively, be then sufficiently mixed uniformly, obtain raising the substrate of Lumbricus；Preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, is joined by 0.5L sodium selenite aqueous solution in the substrate of the 12.5kg weight obtained；In the another substrate that a certain amount of water is joined the other 12.5kg weight obtained；Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust；The selenium-rich breeding dust obtained and common breeding dust introduce Lumbricus, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 15 days respectively, earthworm breeding is after 40 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm；Selenium-enriched earthworm bowel relieving 20 hours under dark condition that will obtain, then clean with clear water, clean selenium-enriched earthworm tissue refiner are carried out homogenizing homogenate；The multiple enzyme that Lumbricus self contains is utilized to carry out self-dissolving enzymolysis under 50 DEG C of conditions the Lumbricus homogenate obtained, every stirring in 5 hours once, enzymolysis 20 hours, obtain selenium-rich amino acid nutrition stock solution；The common wormcast obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5, it is sufficiently stirred at ambient temperature, it is filtered after stirring 3 hours, it is filtrated to get wormcast extracting solution, wormcast extracting solution is mixed by 1:0.8 volume ratio with selenium-rich amino acid nutrition stock solution, by mixed liquor sterilizing 1.5 hours under 100 DEG C of conditions, then cooling, filtration, obtaining selenium-enriched earthworm fertilizer selenium, its Se content is about 8.2mg/L, and wherein organic selenium content accounts for 96%.
Embodiment 3
Make selenium-enriched earthworm fertilizer and include step: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, cross 5mm sieve respectively, be then sufficiently mixed uniformly, obtain raising the substrate of Lumbricus；Preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, is joined by 1.0L sodium selenite aqueous solution in the substrate of the 25kg weight obtained；In the another substrate that a certain amount of water is joined the other 25kg weight obtained；Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust；The selenium-rich breeding dust obtained and common breeding dust introduce Lumbricus, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 20 days respectively, earthworm breeding is after 50 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm；Selenium-enriched earthworm bowel relieving 26 hours under dark condition that will obtain, then clean with clear water, clean selenium-enriched earthworm tissue refiner are carried out homogenizing homogenate；The multiple enzyme that Lumbricus self contains is utilized to carry out self-dissolving enzymolysis under 60 DEG C of conditions the Lumbricus homogenate obtained, every stirring in 6 hours once, enzymolysis 26 hours, obtain selenium-rich amino acid nutrition stock solution；The common wormcast obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:6, it is sufficiently stirred at ambient temperature, it is filtered after stirring 5 hours, it is filtrated to get wormcast extracting solution, wormcast extracting solution is mixed by 1:1.2 volume ratio with selenium-rich amino acid nutrition stock solution, by mixed liquor sterilizing 2.5 hours under 100 DEG C of conditions, then cooling, filtration, obtaining selenium-enriched earthworm fertilizer, its Se content is about 12.1mg/L, and wherein organic selenium content accounts for 97.5%.
Embodiment of above is merely to illustrate the present invention, but not limitation of the present invention.Although the present invention being described in detail with reference to embodiment, it will be understood by those within the art that, technical scheme is carried out various combination, amendment or equivalent replacement, without departure from the spirit and scope of technical solution of the present invention, all should be encompassed in the middle of scope of the presently claimed invention.
Claims (8)
1. the manufacture method of a selenium-enriched earthworm fertilizer, it is characterised in that the method comprises the steps:
Step one: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, crosses 3-5mm sieve respectively, is then sufficiently mixed uniformly, obtains raising the substrate of Lumbricus；
Step 2: preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, joins the sodium selenite aqueous solution of 0.5-1.0L in the substrate of the 12.5-25kg weight that step one obtains；In the another substrate that a certain amount of water is joined the other 1.55-25kg weight that step one obtains；Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust；
Step 3: introduce Lumbricus in the selenium-rich breeding dust obtained in step 2 and common breeding dust, raise at 22-28 DEG C of temperature, once identical with former substrate substrate is added respectively every 15-20 days, after earthworm breeding 40-50 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm, standby；
Step 4: selenium-enriched earthworm step 3 obtained is bowel relieving 20-26 hour under dark condition, then cleans with clear water, clean selenium-enriched earthworm tissue refiner is carried out homogenizing homogenate, obtains Lumbricus homogenate；
Step 5: Lumbricus homogenate step 4 obtained utilizes the multiple enzyme that Lumbricus self contains to carry out self-dissolving enzymolysis under 50-60 DEG C of condition, every stirring in 5-6 hour once, enzymolysis 20-26 hour, obtain selenium-rich amino acid nutrition stock solution；
Step 6: common wormcast step 3 obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5-6, it is sufficiently stirred at ambient temperature, it is filtered after stirring 3-5 hour, it is filtrated to get wormcast extracting solution, the selenium-rich amino acid nutrition stock solution that above-mentioned wormcast extracting solution obtains with step 5 is mixed according to a certain percentage, by mixed liquor sterilizing 1.5-2.5 hour under 100 DEG C of conditions, then cooling, filtration, to obtain final product.
2. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that described feces of livestock and poultry is cattle manure.
3. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that described dreg is the residue of mushroom culture medium material, it is preferable that the residue of culture medium for golden mushroom material.
4. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that described Lumbricus is girdled adult Eisenia foetida.
5. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that in step 6, described wormcast extracting solution mixes according to volume ratio 1:0.8-1.2 with selenium-rich amino acid nutrition stock solution.
6. the manufacture method of selenium-enriched earthworm fertilizer according to claim 1, it is characterised in that the method comprises the steps:
Step one: weigh the feces of livestock and poultry after fermentation and dreg according to the ratio that mass ratio is 1:1, crosses 4mm sieve respectively, is then sufficiently mixed uniformly, obtains raising the substrate of Lumbricus；
Step 2: preparation quality concentration is the sodium selenite aqueous solution of 3.33g/L, joins 0.8L sodium selenite aqueous solution in the substrate of the 20kg weight that step one obtains；In the another substrate that a certain amount of water is joined the other 20kg weight that step one obtains；Regulate two parts of water content of substrate all to 65-70%, obtain selenium-rich breeding dust and common breeding dust；
Step 3: introduce Lumbricus in the selenium-rich breeding dust obtained in step 2 and common breeding dust, raising at 22-28 DEG C of temperature, added once identical with former substrate substrate every 18 days respectively, earthworm breeding is after 45 days, separate Lumbricus and wormcast, choose common wormcast and selenium-enriched earthworm；
Step 4: selenium-enriched earthworm step 3 obtained is bowel relieving 24 hours under dark condition, then cleans with clear water, clean selenium-enriched earthworm tissue refiner is carried out homogenizing homogenate；
Step 5: Lumbricus homogenate step 4 obtained utilizes the multiple enzyme that Lumbricus self contains to carry out self-dissolving enzymolysis under 55 DEG C of conditions, every stirring in 5.5 hours once, enzymolysis 24 hours, obtain selenium-rich amino acid nutrition stock solution；
Step 6: common wormcast step 3 obtained and clear water are sufficiently mixed uniformly according to the ratio that mass ratio is 1:5.5, it is sufficiently stirred at ambient temperature, it is filtered after stirring 4 hours, it is filtrated to get wormcast extracting solution, the selenium-rich amino acid nutrition stock solution that above-mentioned wormcast extracting solution obtains with step 5 is mixed according to volume ratio 1:1, by mixed liquor sterilizing 2 hours under 100 DEG C of conditions, then cooling, filtration, to obtain final product.
7. the selenium-enriched earthworm fertilizer that the manufacture method of the selenium-enriched earthworm fertilizer described in claim 1-6 prepares, it is characterised in that its total Se content is about 8-12mg/L, and wherein organic selenium content accounts for more than 96%.
8. selenium-enriched earthworm fertilizer according to claim 7, it is characterised in that its pH value is 6.8, close to neutral.
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| CN106509639A (en) * | 2016-11-18 | 2017-03-22 | 扬州名佳食品有限公司 | Selenium-enriched wheat planting method, and selenium-enriched wheat flour preparation method |
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