CN107173331A - A kind of method that selenium-enriched earthworm is continuously cultivated - Google Patents
A kind of method that selenium-enriched earthworm is continuously cultivated Download PDFInfo
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- CN107173331A CN107173331A CN201710347587.7A CN201710347587A CN107173331A CN 107173331 A CN107173331 A CN 107173331A CN 201710347587 A CN201710347587 A CN 201710347587A CN 107173331 A CN107173331 A CN 107173331A
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- earthworm
- selenium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0332—Earthworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses the method that a kind of selenium-enriched earthworm of field of agricultural production technologies is continuously cultivated.Comprise the following steps：By fermented cow dung and bacteria residue in mass ratio 3:1 is well mixed, and adds sodium selenite aqueous solution, regulation water content, ageing, obtains matter containing seleno；Introduce primary into earthworm culture, isolate primary earthworm；Continue to cultivate the young earthworm in first filial generation, isolate first filial generation into earthworm；Continue to cultivate the young earthworm of second filial, isolate second filial into earthworm, produce the selenium-enriched earthworm of continuous culture three generations.The present invention uses low concentration selenium culture earthworm, and with preferable Se accumulation efficiency, on earthworm yield and breeding potential substantially without influence, Se content increases as earthworm generation-inter- is multiplied, and can retain earthworm active composition to greatest extent using freeze-drying process selenium-enriched earthworm.The density of bacteria residue is smaller in matrix, it is ensured that good gas permeability；The ratio of fermented cow dung is higher, can eliminate the influence that can not be made full use of in the bacteria residue resolving time long and short time by earthworm.
Description
Technical field
The invention belongs to field of agricultural production technologies, and in particular to a kind of method that selenium-enriched earthworm is continuously cultivated.
Background technology
Selenium is that humans and animals body grows required trace element, is also the beneficial element of plant growth.Selenium participation group
A variety of important functional enzymes, play anti-oxidant, anti-aging, detoxication and toxicant eliminating function, cancer-resisting etc. a variety of in human body in into body
Important biological function, to maintaining health and normal physiological metabolism that there is particularly important effect.China's Selenium in Soil
Maldistribution of the resources is weighed and there is the selenium deficiency area of large area, and this directly results in main selenium source --- the selenium in food of human body
Nutrition supply is not enough, and then influences health.
The nutriments such as amino acid, vitamin and the mineral element that earthworm is enriched containing a large amount of protein, species in itself, be
The protein source material and medical material (being commonly called as " earthworm ") of a kind of high-quality.Earthworm Se accumulation amount is big, and can pass through life
Inorganic selenium is converted into Organic Selenium by thing transformation, absorbs enrichment and transformation efficiency is higher.Selenium-enriched earthworm may be used as livestock and poultry, water
The demand of the nutritional need, especially Organic Selenium of the feed of production etc., supplement livestock and poultry and aquatic products；Extraction high activity can also be used as
The medical material of albumen and antineoplastic component, and then for making antineoplastic, with particularly important medical medical value.
The cultural method of current selenium-enriched earthworm is generally the selenium that higher concentration is added in matrix, but high concentration selenium long term can be caused
Earthworm variety deterioration, breeding potential declines, and can only cultivate a generation or two generations, it is impossible to continuously cultivate selenium-enriched earthworm, it is impossible to meet selenium-rich
The long-term cultivation of earthworm and acquisition.
The content of the invention
The invention aims to overcome in the prior art culture selenium-enriched earthworm during earthworm variety deterioration, breeding potential
The problem of declining, can not continuously cultivate, a kind of method that selenium-enriched earthworm is continuously cultivated is proposed for this.
The concrete technical scheme of the present invention is as follows：
A kind of method that selenium-enriched earthworm is continuously cultivated, comprises the following steps：
(1) preparation of the matter containing seleno：By fermented cow dung and bacteria residue in mass ratio 3:1 is well mixed, and then adds selenous acid
Sodium water solution, regulation water content, obtain the matrix that selenium concentration is 10-20mg/kg, matter containing seleno are obtained after ageing；
(2) primary adult earthworm culture is introduced in the matter containing seleno, and contains selenium described in supplement addition in the training period
Primary earthworm is taken out after matrix, the young earthworm in first filial generation to appear or earthworm cocoon, that is, obtains first generation selenium-enriched earthworm；
(3) the young earthworm in first filial generation or earthworm cocoon in step (2) are continued to cultivate, supplement addition is described during culture contains seleno
Matter, takes out first filial generation into earthworm after first filial generation earthworm maturation and after there is the young earthworm of second filial or earthworm cocoon, produces second generation selenium-enriched earthworm；
(4) the young earthworm of second filial or earthworm cocoon in step (3) are continued to cultivate, supplement addition is described during culture contains seleno
Matter, takes out second filial into earthworm after second filial earthworm maturation and after there is the young earthworm of F3 or earthworm cocoon, produces third generation selenium-enriched earthworm.
Bacteria residue described in step (1) is mistake respectively before the residue of mushroom culture medium material, the fermented cow dung and bacteria residue mixing
4mm is sieved.
Water content described in step (1) is 65-70%.
The time being aged described in step (1) is 7-20d, and temperature is 12-28 DEG C.
Primary earthworm described in step (2) is girdled adult Eisenia Foetida.
The temperature cultivated described in step (2) is 12-28 DEG C, and the time is 40-50d.
The temperature cultivated described in step (3) and (4) is 12-28 DEG C, and the time is 45-52d.
It is described supplement addition method be：It was added during culture every 15-20 days.
Selenium-enriched earthworm is cleaned with clear water obtained by step (2)-(4), and gut purge is handled under dark condition, is preserved after freeze-drying.
Beneficial effects of the present invention are：
1st, the present invention uses low concentration selenium culture Eisenia Foetida, earthworm is had preferable Se accumulation efficiency, realizes rich
The continuous culture of selenium earthworm, and on earthworm yield and breeding potential substantially without influence；Earthworm body Se content is numerous with earthworm generation-inter-
Spread out and increase, this also provides possibility to cultivate the higher earthworm kind of Se accumulation amount.
2nd, use fermented cow dung and bacteria residue for matrix, the density of bacteria residue is smaller, ensure that the good gas permeability of matrix；Together
When earthworm Major Foods (fermented cow dung) ratio it is higher, can eliminate can not be by earthworm in the bacteria residue resolving time long and short time
The influence that earthworm makes full use of.The method that selenium-enriched earthworm of the present invention is continuously cultivated is applied to outdoor crop field culture.
3rd, earthworm active composition can be retained to greatest extent using freeze-drying process selenium-enriched earthworm, reduces post-production
During loss.
4th, earthworm body contains antitumor and treatment angiocardiopathy material, and appropriate selenium also has antitumor and anti-oxidant etc.
Important biological function, therefore, the selenium-enriched earthworm that the present invention is harvested are likely to the dual-use function with earthworm and selenium.Using this
The earthworm of invention culture has higher nutritive value, can be widely applied to the production of selenium-enriched earthworm amino acid, can be used as livestock and poultry
And the selenium supplement of the feed such as aquatic products, the selenium-containing active composition therefrom extracted also can be used in the production of antineoplastic, has
Extremely wide application and great application value.
Embodiment
The present invention proposes a kind of method that selenium-enriched earthworm is continuously cultivated, and following examples facilitate a better understanding of this hair
It is bright, but it is not limited to the present invention.
Embodiment 1
The method that selenium-enriched earthworm is continuously cultivated comprises the following steps：
(1) it is 3 according to mass ratio:1 ratio weighs fermented cow dung and bacteria residue, and 4mm sieves are crossed respectively, are then well mixed,
Obtain cultivating the matrix of earthworm；
(2) sodium selenite aqueous solution containing 0.1g selenium is added in the matrix in 10kg steps (1), add water regulation base
Matter water content is aging for one week in 12-28 DEG C to 65%, obtains the matter containing seleno that selenium concentration is 10mg/kg；
(3) culture at a temperature of primary adult earthworm, 12-28 DEG C is introduced in matter containing seleno described in step (2), every 15 days
The addition matter containing seleno, after earthworm is cultivated 40 days, primary earthworm is taken out after first filial generation children's earthworm or earthworm cocoon to appear, that is, obtains the
Generation selenium-enriched earthworm；
Weighed after obtained first generation selenium-enriched earthworm is cleaned with clear water, then gut purge processing 24 is small under dark condition
When, vermis Se content is determined after freeze-drying；
(4) the young earthworm in the first filial generation in step (3) or earthworm cocoon continue to cultivate at a temperature of 12-28 DEG C, and institute was added every 15 days
Matter containing seleno is stated, after cultivating 52 days, first filial generation is taken out into earthworm after first filial generation earthworm maturation and after there is the young earthworm of second filial or earthworm cocoon,
Produce second generation selenium-enriched earthworm；
Weighed after second generation selenium-enriched earthworm is cleaned with clear water, then gut purge is handled 24 hours under dark condition, freezing
Vermis Se content is determined after drying；
(5) the young earthworm of the second filial in step (4) or earthworm cocoon continue to cultivate at a temperature of 12-28 DEG C, and institute was added every 15 days
Matter containing seleno is stated, after cultivating 52 days, second filial is taken out into earthworm after second filial earthworm maturation and after there is the young earthworm of F3 or earthworm cocoon,
Produce third generation selenium-enriched earthworm；
Weighed after third generation selenium-enriched earthworm is cleaned with clear water, gut purge is handled 24 hours under dark condition, freeze-drying
Vermis Se content is determined afterwards.
First, second and third obtained generation earthworm wall scroll weight is respectively 0.52g, 0.49g, 0.48g, freezes earthworm Se content
Respectively 28.10mg/kg, 39.76mg/kg, 61.29mg/kg (Se content/earthworm dry weight), it can be seen that：Low selenium concentration
(10mg/kg) influences little to earthworm biomass, and vermis Se accumulation amount is multiplied and increased with generation-inter-.
Embodiment 2
The method that selenium-enriched earthworm is continuously cultivated comprises the following steps：
(1) it is 3 according to mass ratio:1 ratio weighs fermented cow dung and bacteria residue, and 4mm sieves are crossed respectively, are then well mixed,
Obtain cultivating the matrix of earthworm；
(2) sodium selenite aqueous solution containing 0.2g selenium is added in the matrix in 10kg steps (1), add water regulation base
Matter water content is aged two weeks to 70% in 12-28 DEG C, obtains the matter containing seleno that selenium concentration is 20mg/kg；
(3) culture at a temperature of primary adult earthworm, 12-28 DEG C is introduced in matter containing seleno described in step (2), every 20 days
The addition matter containing seleno, after earthworm is cultivated 40 days, primary earthworm is taken out after first filial generation children's earthworm or earthworm cocoon to appear, that is, obtains the
Generation selenium-enriched earthworm；
Weighed after obtained first generation selenium-enriched earthworm is cleaned with clear water, then gut purge processing 24 is small under dark condition
When, vermis Se content is determined after freeze-drying；
(4) the young earthworm in the first filial generation in step (3) or earthworm cocoon continue to cultivate at a temperature of 12-28 DEG C, and institute was added every 20 days
Matter containing seleno is stated, after cultivating 52 days, first filial generation is taken out into earthworm after first filial generation earthworm maturation and after there is the young earthworm of second filial or earthworm cocoon,
Produce second generation selenium-enriched earthworm；
Weighed after second generation selenium-enriched earthworm is cleaned with clear water, then gut purge is handled 24 hours under dark condition, freezing
Vermis Se content is determined after drying；
(5) the young earthworm of the second filial in step (4) or earthworm cocoon continue to cultivate at a temperature of 12-28 DEG C, and institute was added every 20 days
Matter containing seleno is stated, after cultivating 52 days, second filial is taken out into earthworm after second filial earthworm maturation and after there is the young earthworm of F3 or earthworm cocoon,
Produce third generation selenium-enriched earthworm；
Weighed after third generation selenium-enriched earthworm is cleaned with clear water, gut purge is handled 24 hours under dark condition, freeze-drying
Vermis Se content is determined afterwards.
The first, second and third generation wall scroll earthworm biomass obtained is respectively 0.51g, 0.49g, 0.46g, earthworm body Se content
Respectively 36.38mg/kg, 90.73mg/kg, 117.26mg/kg (Se content/earthworm dry weight), it can be seen that：Low selenium concentration
(20mg/kg) influences little to earthworm biomass, and vermis Se accumulation amount is multiplied and increased with generation-inter-.
Claims (9)
1. a kind of method that selenium-enriched earthworm is continuously cultivated, it is characterised in that comprise the following steps：
(1) preparation of the matter containing seleno：By fermented cow dung and bacteria residue in mass ratio 3:1 is well mixed, and then adds sodium selenite water
Solution, regulation water content, obtain the matrix that selenium concentration is 10-20mg/kg, matter containing seleno are obtained after ageing；
(2) primary adult earthworm culture, and the supplement addition matter containing seleno in the training period are introduced in the matter containing seleno,
Primary earthworm is taken out after the young earthworm in first filial generation to appear or earthworm cocoon, that is, obtains first generation selenium-enriched earthworm；
(3) the young earthworm in first filial generation or earthworm cocoon in step (2) are continued to cultivate, the supplement addition matter containing seleno, is treated during culture
First filial generation earthworm is ripe and occurs taking out first filial generation after second filial children's earthworm or earthworm cocoon into earthworm, produces second generation selenium-enriched earthworm；
(4) the young earthworm of second filial or earthworm cocoon in step (3) are continued to cultivate, the supplement addition matter containing seleno, is treated during culture
Second filial earthworm is ripe and occurs taking out second filial after F3 children's earthworm or earthworm cocoon into earthworm, produces third generation selenium-enriched earthworm.
2. according to the method described in claim 1, it is characterised in that bacteria residue described in step (1) is residual for mushroom culture medium material
4mm sieves are crossed respectively before slag, the fermented cow dung and bacteria residue mixing.
3. according to the method described in claim 1, it is characterised in that water content described in step (1) is 65-70%.
4. according to the method described in claim 1, it is characterised in that the time being aged described in step (1) is 7-20d, temperature
For 12-28 DEG C.
5. according to the method described in claim 1, it is characterised in that primary earthworm described in step (2) is girdled adult
Eisenia Foetida.
6. when according to the method described in claim 1, it is characterised in that the temperature cultivated described in step (2) is 12-28 DEG C,
Between be 40-50d.
7. according to the method described in claim 1, it is characterised in that the temperature cultivated described in step (3) and (4) is 12-28
DEG C, the time is 45-52d.
8. according to the method described in claim 1, it is characterised in that the method for the supplement addition is：Every 15- during culture
It is added within 20 days.
9. according to the method described in claim 1, it is characterised in that selenium-enriched earthworm is cleaned with clear water obtained by step (2)-(4), black
Gut purge is handled under dark condition, is preserved after freeze-drying.
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Cited By (6)
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| CN107996521A (en) * | 2017-12-05 | 2018-05-08 | 沈阳尊龙生物技术有限公司 | The cultural method and accessory substance selenium-enriched earthworm excrement of a kind of selenium-enriched earthworm |
| CN108077190A (en) * | 2017-12-29 | 2018-05-29 | 黄河三角洲京博化工研究院有限公司 | A kind of selenium-enriched earthworm powder and its application |
| CN108719207A (en) * | 2018-05-11 | 2018-11-02 | 李大冶 | A method of standardization breeding earthworm |
| CN108782437A (en) * | 2018-05-29 | 2018-11-13 | 仁怀市瑞博养殖场 | A kind of Gallus domesticlus brisson cultural method |
| CN111011868A (en) * | 2019-10-09 | 2020-04-17 | 江西沐恩堂生物科技有限公司 | Selenium-rich earthworm compound protein powder and preparation method thereof |
| CN113039989A (en) * | 2021-03-26 | 2021-06-29 | 安徽大学 | Intelligent ecological planting method for changing selenium-rich orchard into soil and controlling grass in hilly and mountainous areas |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107996521A (en) * | 2017-12-05 | 2018-05-08 | 沈阳尊龙生物技术有限公司 | The cultural method and accessory substance selenium-enriched earthworm excrement of a kind of selenium-enriched earthworm |
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| CN108782437A (en) * | 2018-05-29 | 2018-11-13 | 仁怀市瑞博养殖场 | A kind of Gallus domesticlus brisson cultural method |
| CN111011868A (en) * | 2019-10-09 | 2020-04-17 | 江西沐恩堂生物科技有限公司 | Selenium-rich earthworm compound protein powder and preparation method thereof |
| CN113039989A (en) * | 2021-03-26 | 2021-06-29 | 安徽大学 | Intelligent ecological planting method for changing selenium-rich orchard into soil and controlling grass in hilly and mountainous areas |
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| CN107173331B (en) | 2019-12-10 |
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