KR101057500B1 - Method of cultivating oyster mushroom with high sulfur content and oyster mushroom grown by the method - Google Patents

Abstract

The present invention relates to a method for cultivating oyster mushrooms containing a large amount of sulfur components and oyster mushrooms cultivated by the method, and more particularly, to prepare a medium containing sulfur constituents when cultivating oyster mushrooms and inoculating the seedling mushroom seedlings therein. By culturing, the present invention relates to a method for cultivating oyster mushrooms and to cultivate oyster mushrooms by the method. Oyster mushroom cultivation method according to the present invention and the oyster mushroom cultivated by the method can be ingested sulfur beneficial to the human body at the same time by ingesting the oyster mushroom, so easy to ingest the beneficial sulfur component to the human body Make it possible.

Oyster mushroom, sulfur, medium, culture, spawn

Description

Method for cultivating high concentrative sulfur containing agaric mushroom, and agaric mushroom cultivated by the same}

The present invention relates to a method for cultivating oyster mushrooms containing a large amount of sulfur components and oyster mushrooms cultivated by the method, and more particularly, to prepare a medium containing sulfur constituents when cultivating oyster mushrooms and inoculating the seedling mushroom seedlings therein. By culturing, the present invention relates to a method for cultivating oyster mushrooms so that the harvested oyster mushrooms contain a large amount of sulfur components beneficial to the human body, and cultivated oyster mushrooms by the method.

Sulfur constitutes the human body in the eighth largest amount among several kinds, including hydrogen, oxygen, nitrogen, sulfur, sodium, etc., which are a large amount of biological elements constituting the human body. In particular, it is widely distributed in bones, skin, and hair, and sulfur deficiency is known to cause baldness, keratinization of nails and toenails, and aging of the skin.

In addition, sulfur has a detoxification effect which can be freed from pollution of pollutants such as heavy metals, chemicals, and various pesticides, which are the biggest enemies of human health, and thus, it is not an essential nutrient for human body.

As described above, sulfur is known to improve human health because it has the effect of treating and preventing human diseases, but in general, the intake of sulfur is garlic, ginger, leek, onion, radish, soothing and leek. It is made by ingesting foods containing natural sulfur components such as doenjang and so on. Since there is not enough intake of sulfur essential to the human body, development of various methods of ingesting sulfur is required.

Meanwhile, Pleurotus eryngii is a fungus of the genus Pleurotus eryngii (Pleurotus eryngii), which is distributed in many countries around the world, including Korea, Japan, and Taiwan, and contains large amounts of protein, fat, soluble nitrogen, ash, and vitamins. In addition, the pharmacological components contained in oyster mushrooms are effective for biodefence, maintenance of bio-conditions, control of constitutional rhythms, disease recovery, and prevention and improvement of adult diseases, and sugars having anti-cancer and antimicrobial effects are known. As it contains a large amount, its demand is steadily increasing as a national health food.

Therefore, while the present inventors are studying the cultivation method of sulfur-containing Pleurotus eryngii, while developing a culture medium containing sulfur during the mushroom cultivation process, the medium is applied to the Pleurotus mushroom to complete the cultivation method of Pleurotus eryngii containing a large amount of sulfur As a result, as described above, the lychee mushroom, which is beneficial to the human body, contains a large amount of sulfur essential for the composition of the human body, thereby providing a method of consuming the oyster mushroom and the sulfur at the same time.

It is an object of the present invention to provide a method for containing a large amount of sulfur components in the oyster mushroom, so that it can be easily ingested sulfur components beneficial to the human body.

In order to achieve the above object, the present invention comprises the steps of preparing a wet medium by adding 0.5 to 5 parts by weight of sulfur, 0.2 to 0.4 parts by weight of stevia concentrate and 250 to 350 parts by weight of water based on 1000 parts by weight of dry medium; Filling the wet medium in a culture bottle 60 to 70% by volume, sterilizing for 5 to 7 hours with 100 ℃ steam; Cooling the sterilized culture bottle to room temperature and then inoculating the seedling mushroom seedlings into the wet medium in the culture bottle; Culturing the seeded seedlings at 20-25 ° C. for 20-25 days; And germinating the cultured bacteria after germinating the bacteria and growing them at 15 to 18 ° C. for 5 to 10 days.

At this time, the dry paper is preferably prepared by mixing 60 to 70 parts by weight of cottonseed hull (cottonseed hull), 20 to 30 parts by weight of beet mill and 20 to 30 parts by weight of cottonseed meal (cottonseed meal).

In addition, in the inoculation method of inoculating the Pleurotus eryngii spawn in the culture medium, the seed is inoculated with 1.8 to 2.2 parts by weight based on 100 parts by weight of the wet medium, 55 to 65% by weight of the spawned inoculation Preferably it is mixed and 35 to 45% by weight is inoculated on the surface of the wet medium.

In addition, the humidity in the germination and growth stage, 95-98% during germination, 85-90% after germination, it is preferable to maintain at 80-85% during the harvest.

The present invention also provides oyster mushrooms grown by the above method.

At this time, the Oyster mushroom has a sulfur content of more than 450ppm.

Oyster mushroom cultivation method according to the present invention and the oyster mushroom cultivated by the method can be ingested sulfur beneficial to the human body at the same time by ingesting the oyster mushroom, so easy to ingest the beneficial sulfur component to the human body Make it possible.

Hereinafter, a method of growing oyster mushroom with a high sulfur content according to the present invention will be described.

1 is a flow chart of cultivation of oyster mushroom according to the present invention.

First, prepare 100 parts by weight of sawdust to prepare a mushroom cultivation medium according to the present invention, 60 to 70 parts by weight of cotton hull (cottonseed hull), 20 to 30 parts by weight of beet wheat and 20 to 30 cottonseed meal (cottonseed meal) A dry medium is prepared by mixing parts by weight. Next, based on 1000 parts by weight of the dry broth, add 0.5 to 5 parts by weight of sulphurized sulfur, 0.2 to 0.4 parts by weight of stevia concentrate and 250 to 350 parts by weight of water, and stir for at least 3 hours. The wet medium is prepared by allowing the mixture to mix evenly.

Stevia is a perennial plant, which contains carotene, vitamins, proteins, calcium, carbohydrates, glutamine and organic carbon, and when administered to crops, prevents pests due to active microorganisms in soil, enhances intelligence, increases yield, It has many advantages as a natural fertilizer such as root lubrication, increased sugar content and improved shelf life. In particular, there is an antioxidant activity corresponding to five times the green tea and sugar of 200 to 300 times the sugar, when used in the medium of the present invention serves to double the efficacy of the anticancer and antibacterial activity of the oyster mushroom according to the present invention.

Next, the wet medium is filled with 60 to 70% by volume of the culture bottle, but if the filling amount is less than 60% by volume, the spawn inoculation amount of the following process decreases, and thus the economical efficiency is lowered. When the volume exceeds 70% by volume, the ventilation is poor due to insufficient air gap in the medium. As a result, the culture is delayed and the growth of mycelia is not good, resulting in poor germination and imbalance of growth after germination.

After filling the culture bottle with the wet medium, sterilize it with 100 ℃ water vapor for 5-7 hours. At this time, care should be taken not to overheat the medium by steam.

After cooling the sterilized culture bottle to room temperature and inoculating the seedling mushroom seedlings in the culture medium in the culture bottle, based on 100 parts by weight of the wet medium seed spawn 1.8 ~ 2.2 parts by weight, 55 ~ 65% by weight of the spawn seed It is preferable to mix the wet medium and inoculate 35 to 45% by weight on the surface of the wet medium.

After spawn inoculation, it is preferably incubated at 20-25 ° C. for 20-25 days, followed by scraping of the cultured bacteria. Bacterial scraping is carried out for the purpose of removing the inoculum inoculum and injuring the hyphae and using the regeneration power to promote mushroom development.

After germination, germinate in germination chamber and grow at 15 ~ 18 ℃ for 5 ~ 10 days. If the growth temperature is less than 15 ° C shortening the mushroom stand and the mushroom generation is sluggish, if the growth temperature exceeds 18 ° C it is preferable to maintain the growth temperature.

In addition, the humidity is preferably maintained at 95 to 98% during germination, 85 to 90% after germination, 80 to 85% during the harvesting period. If the humidity is less than the above range, the germination and growth are poor. Mycelia may grow or deformed mushrooms may develop.

In addition, it is preferable to ventilate in order to prevent obstacles caused by carbon dioxide, and it is also preferable to increase the ventilation amount as the growth progresses.

Mushrooms should be harvested before spores are scattered so that they have good taste and nutrition.

Hereinafter, the sulfur cultivation method having a high sulfur content will be described in more detail through the following examples and experimental examples.

However, the following examples are only for illustrating the present invention, and the present invention is not limited to the following examples and may be changed to other embodiments equivalent to substitutions and equivalents without departing from the technical spirit of the present invention. Will be apparent to those of ordinary skill in the art.

<Examples>

First, a dry medium was prepared by mixing 300 kg of cotton thread 300 kg, 100 kg of beet wheat, and 100 kg of cotton thread foil to 450 kg of sawdust, and 3 kg of liquid sulfur (30% by weight of sulfur) formulated on the dry medium, 300 g of stevia concentrate, and After adding 300 kg of water, the mixture was stirred for 3 hours to prepare a wet medium. The sawdust, cotton thread, beet mill, cottonseed gourd, sulfur and stevia concentrate were purchased from commercially available materials, in particular sawdust used domestic wood-made material, foreign imported sea salts The use of sawdust made of wood was ruled out.

Ten culture medium samples prepared by adhering 550 g of the wet medium to a 850 ㏄ culture bottle were sterilized with 100 ° C. steam for 6 hours, and then cooled to room temperature. At this time, the inoculation was carried out immediately after manufacturing the wet medium to minimize the infiltration of other bacteria except the Pleurotus eryngii spawn.When sterilization and cooling, the humidity of the medium is increased by condensation of water vapor by pre-cooling to 75 ℃ and then cooling to room temperature. Was suppressed.

Next, after inoculating 6.5 g of Pleurotus erythritis in each of the culture mediums, the mixture was inoculated, and then inoculated with 4.5 g of Pleurotus spawn on the surface of the wet medium, and incubated at 23 ° C. for 23 days.

The cultured bacteria were germinated at a humidity of 96% after scraping the bacteria, and grown at a temperature of 16 ° C. gradually decreasing from 88% to 82% of humidity, and harvested after 7 days.

Experimental Example

Using the commercially available Pleurotus eryngii as a control, the sulfur content of the Pleurotus eryngii harvested in the above example was analyzed by Konkuk University Animal Resource Research Center and the results are shown in Table 1 below.

Sulfur Content Analysis of Pleurotus eryngii Sulfur content (ppm) Control 289.02 Example One 453.60 2 455.85 3 461.10 4 455.42 5 461.96 6 458.56 7 452.81 8 451.68 9 468.57 10 454.63

As shown in Table 1, the oyster mushroom cultivated by the method according to the present invention was found to contain approximately 1.5 times more sulfur than the common oyster mushroom.

As described above, the present invention is prepared by incubating the medium containing sulfur components when cultivating oyster mushrooms and inoculating them with the seedlings of oyster mushrooms, so that the harvested oyster mushrooms contain a large amount of sulfur components beneficial to the human body, Ingestion of oyster mushrooms cultivated according to the invention and at the same time can be ingested with sulfur, it is possible to easily ingest the sulfur components beneficial to the human body without ingesting sulfur components separately.

1 is a cultivation flowchart of oyster mushroom according to the present invention.

Claims (6)

Preparing a wet medium by adding 0.5-5 parts by weight of sulfur, 0.2-0.4 parts by weight of stevia concentrate and 250-350 parts by weight of water, based on 1000 parts by weight of dry medium; Filling the wet medium in a culture bottle 60 to 70% by volume, sterilizing for 5 to 7 hours with 100 ℃ steam; Cooling the sterilized culture bottle to room temperature and then inoculating the seedling mushroom seedlings into the wet medium in the culture bottle; Culturing the seeded seedlings at 20-25 ° C. for 20-25 days; And After germinating the cultured bacteria germ germination and growing for 5 to 10 days at 15 ~ 18 ℃, sulfur content high oyster mushroom cultivation method. The method of claim 1, The dry paper is a sulfur-rich oyster mushroom cultivation method, characterized in that it is prepared by mixing 60 to 70 parts by weight cotton swab, 20 to 30 parts by weight and 20 to 30 parts by weight of cottonseed foil. The method of claim 1, In the inoculation method of inoculating the oyster mushroom spawn in a culture medium in the culture bottle, based on 100 parts by weight of the wet medium spawn 1.8 to 2.2 parts by weight, 55 to 65% by weight of the spawn seed is mixed with the wet medium 35-45% by weight is inoculated on the surface of the wet medium, high sulfur content oyster mushroom cultivation method. The method of claim 1, Humidity in the germination and growth stage, 95 to 98% during germination, 85 to 90% after germination, characterized in that it is maintained at 80 to 85% during the harvest, high sulfur content oyster mushroom cultivation method. Pleurotus cultivated by the method of any one of claims 1 to 4. The method of claim 5, Oyster mushroom, characterized in that the sulfur content of the oyster mushroom exceeds 450ppm.

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