KR100671498B1 - Sulfur mushroom cultivation method - Google Patents

Sulfur mushroom cultivation method

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KR100671498B1
KR100671498B1 KR1020050124032A KR20050124032A KR100671498B1 KR 100671498 B1 KR100671498 B1 KR 100671498B1 KR 1020050124032 A KR1020050124032 A KR 1020050124032A KR 20050124032 A KR20050124032 A KR 20050124032A KR 100671498 B1 KR100671498 B1 KR 100671498B1
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sulfur
medium
weight
water
mushroom
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KR1020050124032A
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Korean (ko)
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전석진
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김연옥
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/20—Culture media, e.g. compost
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
    • C05F11/04—Horticultural earth from peat
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

Provided is a method for cultivating sulfur mushroom (Pleurotus eryngii) to allow the sulfur useful to the human body to be taken in easily when mushroom is taken in. The method comprises the steps of diluting 12 kg of the sulfur where bismuth and heavy metals are removed in 12 L of the water boiling at a temperature of 114 deg.C or more for 5 min to prepare a first sulfur diluted solution, and mixing the first sulfur diluted solution with 500 L of water at 20 deg.C to prepare a second sulfur diluted solution; mixing 520 L of the second sulfur diluted solution with 900 L of water, and spraying the obtained solution to 1,600 kg of the dry medium which comprises 35-40 wt% of corn cob, 25-30 wt% of sawdust, 25 wt% of rice bran, 8 wt% of wheat husks and 0.7 wt% of calcium carbonate and has a water content of 20-25 %, to prepare a wet medium having a water content of 69%; and cultivating Pleurotus eryngii by using the wet medium.

Description

유황 새송이 버섯 재배방법{Sulfur mushroom cultivation method}Sulfur mushroom cultivation method

도 1은 본 발명의 새송이 버섯 재배 흐름도1 is a flow chart of the mushroom cultivation of the present invention

[도면의 주요 부분에 대한 부호의 설명][Description of Symbols for Main Parts of Drawing]

10 : 유황 희석액 제조공정 20 : 배지 제조공정10: sulfur dilution liquid production process 20: medium production process

30 : 입병공정 40 : 살균 및 냉각공정30: enlistment process 40: sterilization and cooling process

50 : 접종공정 60 : 배양공정50: inoculation step 60: culture step

70 : 균 긁기 및 발아공정 80 : 생육공정70: bacteria scraping and germination process 80: growth process

본 발명은 유황 성분이 포함되는 새송이 버섯을 재배하는 방법에 관한 것으로, 새송이 버섯을 먹는 것만으로 유황을 섭취할 수 있도록 하는 것이다.The present invention relates to a method of cultivating the mushrooms Pleurotus containing the sulfur component, so that the sulfur can be ingested only by eating the mushrooms.

새송이 버섯은 '서양의 송이'라는 이름처럼 서양에서는 우리나라의 송이버섯만큼이나 대접을 잘 받는 버섯으로서, 소화를 돕고 정신을 맑게 하고, 비타민 D와 비타민 B2, 타이로시나제, 엽산 등을 많이 함유하고 있어 고혈압 예방과 빈혈 치료에 효과가 있으며, 전분이 없어 당뇨병과 비만에도 좋아 많은 재배가 이루어지고 있다.Pleurotus eryngii, as the name of 'Muscle of the West', is a mushroom that is well treated in the West as much as Korea's matsutake mushroom, which helps digestion and clears the mind, and contains a lot of vitamin D, vitamin B2, tyrosinase and folic acid. It is effective in the prevention of hypertension and anemia treatment, and because there is no starch, it is good for diabetes and obesity.

유황은 우리 몸속에서 8번째로 큰 비중을 차지하고 있는 것으로, 통증 완화 작용이 강하고, 염증을 식히는 작용이 강하고, 물질을 흡착하여 운반하는 성질이 강해 피부 인체의 세포막 깊숙이 투과되어 악성을 발휘하고, 노화방지 및 장의 연동작용을 회복시켜 변비를 회복시키고, 콜리젠의 교차 결합과정을 변화시켜 빠르게 흉터조직을 감소시키는 생리적 작용을 하게 되며, 현재 미국 대체 의학 병원에서는 유황 캡슐이 항암제, 해독제, 근육통 치료제, 통증 완화제, 항우울제, 염증 치료제, 피부 외용제 등으로 다양하게 사용되고 있다.Sulfur occupies the eighth largest proportion in our body, and it has a strong pain relieving effect, a strong effect of cooling inflammation, and a strong ability to adsorb and transport substances. Prevents and restores intestinal peristalsis to restore constipation, and changes the cross-linking process of collagen to physiological action to quickly reduce scar tissue. Currently, in the US alternative medicine hospital, sulfur capsules are used as anticancer, antidote, muscle pain treatment, It is widely used as a pain relief agent, an antidepressant, an inflammation treatment agent, or an external skin preparation.

이러한 새송이 버섯은 자체의 성질만을 갖게 재배되고, 상기 된 중요한 역할의 유황은 각종 채소나 식물을 통하여 일부씩 섭취하고 있는 실정으로, 유황의 중요도로 볼 때 상당히 섭취량이 적은 것이며, 특히 새송이 버섯을 먹음으로서 유성성분의 섭취는 극히 미미한 것이었다.These mushrooms are cultivated with their own properties, and the sulfur of the above-mentioned important role is ingested in part through various vegetables and plants, and in view of the importance of sulfur, the intake of the mushrooms is particularly small. Ingestion of oily ingredients was minimal.

본 발명은 유황 성분이 함유되게 새송이 버섯을 재배하여 새송이 버섯을 먹으면 유황도 섭취하게 되어 별도로 유황섭취에 신경 쓰지 않아도 충분한 유화의 섭취가 가능하여 유황 섭취가 용이한 것이다.The present invention is cultivated mushrooms so that the sulfur component to contain the edible mushrooms to eat the sulfur is also intake of sulfur is easy to consume enough emulsification without worrying about sulfur intake separately.

이러한 본 발명은 비소나 중금속 등이 제거된 정제된 유황을 희석하여 침전되지 않게 한 유황 희석액을 통상의 새송이 배지에 물에 혼합시켜 수분흡수를 시킴으로서 배지에서 재배되는 새송이 버섯에 유황이 함유되는 것으로, 새송이 버섯을 먹으면 자연히 유황을 섭취할 수 있게 된다.In the present invention, sulfur is contained in the Pleurotus cultivar grown in the culture medium by mixing the sulfur dilution solution which is not precipitated by diluting the purified sulfur from which arsenic or heavy metals are removed and so on, in a conventional Pleurotus medium to absorb water. Eating mushrooms will naturally give you sulfur.

본 발명은 비소나 중금속 등이 제거된 정제된 유황을 희석시킨 유황 희석액을 물과 혼합하여 새송이 배지에 뿌려준 후 유황 희석액이 혼합된 물로 함수율을 맞춘 배지에서 새송이 버섯을 배재함으로서 새송이 버섯과 함께 유황을 섭취할 수 있는 것이다.The present invention is a sulfur dilution of diluted sulfur with purified arsenic or heavy metals, such as water and sprinkled on the Pleurotus sulcus medium, and then the sulfur together with the Pleurotus eryngii by releasing the Pleurotus eryngii in the medium with the sulfur dilution mixed water It can be ingested.

이러한 본 발명은 비소나 중금속 등이 제거되어 정제된 유황으로 유황 희석액을 만드는 유황 희석액 제조 공정(10)을 거치고, 상기 된 유황 희석액을 물과 혼합하여 새송이 배지에 뿌려주는 배지제조 공정(20)을 거치고, 상기 된 배지는 재배용 병에 넣어주는 입병공정(30)을 거치고, 병에 넣어진 배지는 고압 살균 및 멸균 처리하여 냉각시키는 살균 및 냉각공정(40)을 거치고, 살균 및 냉각 처리된 배지에 버섯균을 접종하는 접종공정(50)을 거치고, 버섯균이 접종된 배지를 배양하는 배양공정(60)을 거치고, 일정기간 배양되면 버섯자실체를 발아시키는 균긁기 및 발아공정(70)을 거치고, 버섯 자실체의 발아가 완료되면 버섯을 일정 크기로 생육시키는 생육공정(80)을 거침으로서 유황 새송이 버섯의 재배가 이루어진다.The present invention goes through a sulfur dilution manufacturing process (10) to make a sulfur dilution with purified sulfur by removing arsenic, heavy metals, etc., and a medium manufacturing process (20) of mixing the sulfur dilution with water and sprinkling on a Pleurotus medium After passing through the incubation process (30) to put the culture medium in the bottle for cultivation, the bottled medium goes through a sterilization and cooling process (40) to cool by autoclaving and sterilization, and to the sterilized and cooled medium After the inoculation step (50) for inoculating mushrooms, the culture step (60) for culturing the medium inoculated mushrooms, go through the bacteria scraping and germination process (70) to germinate the mushroom fruit body for a certain period of time, When the germination of the mushroom fruiting body is completed, the cultivation of sulfur mushrooms is carried out by going through the growth process 80 to grow the mushrooms to a certain size.

이 같이 유황 희석액 제조 공정(10), 배지제조 공정(20), 입병공정(30), 살균 및 냉각공정(40), 접종공정(50), 배양공정(60), 균긁기 및 발아공정(70), 생육공정(80)을 거쳐 재배되는 유황 새송이 버섯의 재배 과정을 각 공정별로 상세히 살펴보면 다음과 같다.Thus, sulfur dilution manufacturing process (10), medium production process (20), bottle disease process (30), sterilization and cooling process (40), inoculation process (50), culture process (60), bacteria scraping and germination process (70) ), The sulfur cultivation process of sulfur cultivation mushrooms grown through the growth process (80) is described in detail for each process as follows.

1. 유황 희석액 제조공정(10)1. Sulfur Diluent Manufacturing Process (10)

정제된 유황(비소, 중금속이 제거된 유황)분말 12Kg을 114℃이상　끓는 물 20ℓ에 희석('1차 유황 희석액'이라 칭함)하여 5분 정도 유지한 다음, 상기 1차 유황 희석액을 20℃ 정도의 온도를 갖는 약 500ℓ의 물에 다시 혼합('2차 유황 희석액'이라 칭함)한 후 배지에 뿌리기 전에 흔들어 사용하도록 한다.12Kg of refined sulfur (arsenic, heavy metals removed) powder was diluted in 20 liters of boiling water over 114 ° C (referred to as 'primary sulfur dilution') and maintained for about 5 minutes, and then the primary sulfur dilution was kept at about 20 ° C. It is mixed again with about 500 liters of water having a temperature of (referred to as 'secondary sulfur diluent') and shaken before spraying on the medium.

본 발명에서 사용되는 유황은 비소나 중금속 등이 제거된 것을 사용하는 것으로, 시중에 이러한 정제된 유황의 판매가 이루어지고 있어 이를 구입하여 사용하게 된다.Sulfur used in the present invention is to use the arsenic or heavy metals are removed, such as the sale of purified sulfur on the market is to buy and use it.

2. 배지제조 공정(20)2. Medium production process (20)

콘코브 35~40중량%, 톱밥 25~30중량%, 미강 25중량%, 소맥피 8중량%, 탄산칼슘 0.7중량%의 혼합물로 이루어지고 수분함량 20~25%를 갖는 총중량 1600Kg의 건배지를 균일하게 1차 혼합한 뒤 혼합기에 투입하여 혼합기를 가동하면서 관수와 동시에 유황 희석액 제조공정(10)에서 제조된 2차 유황 희석액을 혼합하여 배지 함수율이 69%가 되도록 조절하여 전체가 고르게 수분이 흡수되어 습배지가 되게 한다.Corn cove consisting of 35-40% by weight, 25-30% by sawdust, 25% by weight of rice bran, 8% by weight of wheat bran, 0.7% by weight of calcium carbonate and uniform weight of 1600Kg dry weight with 20-25% water content. After the first mixing, the mixture is put into the mixer and the mixer is operated while mixing the secondary sulfur diluent prepared in the sulfur diluent manufacturing process (10) with watering and controlling the moisture content of the medium to be 69% so that the whole water is absorbed evenly. Make it a wet medium.

여기서 물 900ℓ에 2차 유황 희석액 520ℓ의 비율로 혼합하여 전술된 수분함량 20~25%의 건배지에 뿌려주되 배지의 수분함량을 측정하여 습배지 함수율이 69%가 되도록 고르게 수분이 흡수되게 한다.Here, the mixture of 900 liters of water and 520 liters of the secondary sulfur diluent is sprinkled on a dry medium having the above-described water content of 20-25%, and the moisture content of the medium is measured so that moisture is evenly absorbed so that the wet medium moisture content is 69%.

따라서 새송이 버섯을 재배하기 위한 습배지에는 유황 희석액이 충분히 흡수되어 있게 된다.Therefore, the sulfur diluent is sufficiently absorbed in the wet medium for growing the Pleurotus eryngii.

3. 입병공정(30)3. Hospitalization Process (30)

습배지에 2차 유황 희석액과 물을 혼합하여 충분히 수분이 흡수되어 함수율이 69%가 되면 습배지를 병에 넣는 입병 작업을 한다.The secondary sulfur dilution and water are mixed with the wet medium to absorb enough moisture, and when the moisture content reaches 69%, the bottle is put into the wet medium.

4. 살균 및 냉각공정(40)4. Sterilization and Cooling Process (40)

입병작업을 거쳐 병에 넣어진 습배지는 고압스팀 살균기에서 102℃를 90분간 유지한 후 123℃에서 60분간 유지하여 멸균처리하고, 즉시 냉각실로 옮겨 17℃~19℃로 급속 냉각시킨다.After being bottled, the wet medium is sterilized by maintaining the temperature at 102 ℃ for 90 minutes in the high pressure steam sterilizer for 60 minutes at 123 ℃, and then immediately transferred to the cooling chamber and rapidly cooled to 17 ℃ ~ 19 ℃.

5. 접종공정(50)5. Inoculation process (50)

살균 및 냉각공정(40)에서 냉각시킨 습배지를 무균실(1㎥당 곰팡이 포자수 300개 이하)로 옮겨 배지에 버섯균을 접종한다.The wet medium cooled in the sterilization and cooling process 40 is transferred to a sterile chamber (300 or less mold spores per 1 ㎥) to inoculate the mushroom bacteria.

6. 배양공정(60)6. Culture process (60)

접종공정(50)에서 접종이 끝난 습배지는 배양실로 옮겨 온도 19℃~21℃, 습도 65%를 유지하여 38일~41일 정도 버섯균사를 배양하되 배양실의 실내 환기를 충분히 하여 이산화탄소가 축적 되는 것을 방지한다.After inoculation process 50, the inoculated wet medium is transferred to the incubation chamber to maintain the temperature of 19 ℃ ~ 21 ℃ and humidity 65% to cultivate mushroom hyphae for about 38 ~ 41 days. To prevent them.

7. 균긁기 및 발아공정(70)7. Bacterial Scratching and Germination Process (70)

상기 접종공정(50)에서의 버섯균 접종원을 제거하고 실내온도 15℃~18℃, 습도 90%이상 되는 생육실에서 병을 거꾸로 뒤집어 놓은 상태로 5~6일간 버섯자실체의 발아를 유도한다.Remove the mushroom inoculum inoculum in the inoculation step 50 and induce the germination of the mushroom fruit body in a state of inverting the bottle upside down in the growth room at room temperature 15 ℃ ~ 18 ℃, humidity 90% or more.

8. 생육공정(80)8. Growth Process (80)

버섯자실체의 발아가 완료되면 다시 병을 뒤집어 놓은 후 온도 12℃~14℃, 습도 50~60%로 유지ㆍ관리하여 새송이 버섯의 줄기의 길이가 7~10㎝, 갓의 직경이 4~5㎝가 되면 수확을 하게 되고, 이렇게 수확된 새송이 버섯은 유황 성분을 포함하게 되어 새송이 버섯과 함께 유황을 섭취하게 되는 것으로,
본원 발명은 전북대학교 바이오식품소재개발 및 산업화연구센터에서 실험한 '유황 새송이 버섯과 일반 새송이 버섯의 성분 분석'에 관한 결과에서 확인할 수 있듯이, 본원 발명에 의한 버섯의 구성 아미노산 조성을 살펴보면
(단위 : 습물기준, mg/g)
아 미 노 산 시 료 명 비 고 일반 새송이 버섯 유황 새송이 버섯 Aspartic acid 1.632 1.726 ＊Threonine 0.861 0.985 필수아미노산 Serine 0.811 0.891 Glutamic acid 2.369 2.164 Proline 0.714 0.820 Glycine 0.841 0.992 Alanine 1.153 1.271 Cystine 0.060 0.121 함황아미노산 ＊Valine 0.923 1.043 필수아미노산 ＊Methionine 0.008 0.009 함황아미노산, 필수아미노산 ＊Isoleucine 0.797 0.886 필수아미노산 ＊Leucine 1.230 1.379 필수아미노산 Tyrosine 0.004 0.010 ＊Phenylalanine 0.803 0.883 필수아미노산 Histidine 1.599 1.771 ＊Lysine 1.086 1.176 필수아미노산 Ammonia 0.497 0.470 Arginine 0.680 0.862
임을 알 수 있으며, 여기서 황을 함유하고 있는 아미노산인 Cystine, Methionine의 값이 증가 되어 있음을 알 수가 있어, 유황을 새송이 버섯의 구성분으로 체화되어 새송이의 섭취는 유황의 섭취가 되는 것입니다. After the germination of the mushroom fruit body is completed, turn the bottle upside down and maintain it at a temperature of 12 ℃ ~ 14 ℃ and a humidity of 50 ~ 60% to keep the stem length of 7 ~ 10㎝ and the diameter of the shade of 4 ~ 5㎝. When it comes to harvesting, the harvested mushrooms will contain sulfur and consume sulfur with the mushrooms.
The present invention, as can be seen in the results of the 'analytical analysis of sulfur Pleurotus eryngii and general Pleurotus eryngii' experimented at the Biofood Material Development and Industrialization Research Center of Chonbuk National University,
(Unit: wet water standard, mg / g)
amino acid Sample Name Remarks Common King Mushroom Sulfur Mushroom Aspartic acid 1.632 1.726 ＊ Threonine 0.861 0.985 Essential amino acid Serine 0.811 0.891 Glutamic acid 2.369 2.164 Proline 0.714 0.820 Glycine 0.841 0.992 Alanine 1.153 1.271 Cystine 0.060 0.121 Sulfuric acid ＊ Valine 0.923 1.043 Essential amino acid ＊ Methionine 0.008 0.009 Sulfur-containing amino acids, essential amino acids ＊ Isoleucine 0.797 0.886 Essential amino acid ＊ Leucine 1.230 1.379 Essential amino acid Tyrosine 0.004 0.010 ＊ Phenylalanine 0.803 0.883 Essential amino acid Histidine 1.599 1.771 ＊ Lysine 1.086 1.176 Essential amino acid Ammonia 0.497 0.470 Arginine 0.680 0.862
It can be seen that, here, the value of sulfur-containing amino acids Cystine and Methionine is increased. Sulfur is embodied as a component of Pleurotus eryngii and the consumption of Pleurotus is the intake of sulfur.

이 같이 본 발명은 정제된 유황 분말을 희석하여 유황 희석액을 만든 후 이를 물과 혼합하여 새송이 버섯 재배용 배지에 뿌려주므로서 상기 배지에서 생육이 이루어진 새송이 버섯에는 유황이 함유되어 있도록 하여 유황 새송이 버섯의 섭취가 가능하도록 하는 것이다.As described above, the present invention dilutes the purified sulfur powder to make a sulfur diluent, and then mixes it with water and sprinkles it on the medium for cultivating mushrooms so that the mushrooms grown on the medium contain sulfur to ingest sulfur mushrooms. Is to make it possible.

본 발명은 정제된 유황분말을 희석시킨 유황 희석액을 물과 혼합하여 새송이 버섯 재배용 배지에 뿌려주어 새송이 버섯에 유황성분이 포함되어 있도록 하는 것으로, 새송이 버섯의 섭취와 함께 인체에 유익한 유황의 섭취도 동시에 이루어지게 된다.The present invention is to mix the sulfur dilution of the diluted sulfur powder with water to sprinkle on the medium for cultivating the mushroom mushroom so that the sulfur component is contained in the mushroom mushroom, the intake of sulfur beneficial to the human body at the same time as the intake of the mushroom mushroom Will be done.

따라서 유황을 별도로 섭취하지 않더라도 새송이 버섯의 섭취로 유황도 함께 섭취할 수 있어 유황 섭취의 편리함과 충분한 유황의 섭취로 보양작용뿐 아니라 해독작용을 하게 된다.Therefore, even if you do not consume sulfur separately, you can ingest the sulfur with the intake of mushrooms, so the convenience of sulfur intake and sufficient intake of sulfur, as well as remedial action and detoxification.

Claims (1)

비소, 중금속이 제거되고 정제된 유황 분말 12Kg을 114℃이상　끓는 물 20ℓ에 희석하여 5분간 유지하여 1차 유황 희석액을 만든 후 상기 1차 유황 희석액을 20℃의 물 500ℓ에 혼합하여 2차 유황 희석액을 만들고,Arsenic and heavy metals were removed and purified 12Kg of sulfur powder was diluted in 20 liters of boiling water over 114 ℃ and kept for 5 minutes to make a primary sulfur dilution. Create 콘코브 35~40중량%, 톱밥 25~30중량%, 미강 25중량%, 소맥피 8중량%, 탄산칼슘 0.7중량%의 혼합물로 이루어지고 수분함량 20~25%를 갖는 건배지 1600kg을 혼합할 때 상기 된 2차 유황 희석액 520ℓ를 물 900ℓ에 혼합하여 배지에 뿌려 습배지를 만들되 상기 습배지의 함수율이 69%가 되게 하며,Corn cove consisting of 35-40% by weight, 25-30% by weight of sawdust, 25% by weight of rice bran, 8% by weight of wheat bran, 0.7% by weight of calcium carbonate and mixing 1600kg of dry media with 20-25% moisture content When the above-mentioned secondary sulfur diluent 520ℓ is mixed with 900ℓ of water and sprinkled on the medium to make a wet medium, the moisture content of the wet medium is 69%, 위와 같이 2차 유황 희석액이 뿌려진 습배지로 입병, 살균 및 냉각, 접종, 배양, 균긁기 및 발아, 생육공정을 거쳐 새송이 버섯을 재배하는 것을 특징으로 하는 유황 새송이 버섯 재배방법.Sulfur Pleurotus cultivation method, characterized in that the cultivation of the mushrooms through the process of disease, sterilization and cooling, inoculation, cultivation, bacteria scraping and germination, growth as a wet medium sprayed with a secondary sulfur dilution as above.
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KR101057500B1 (en) 2008-12-31 2011-08-17 오춘식 Method of cultivating oyster mushroom with high sulfur content and oyster mushroom grown by the method
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