CN110432294A - A kind of bacteriostatic agent of biofermentation, preparation method and application - Google Patents
A kind of bacteriostatic agent of biofermentation, preparation method and application Download PDFInfo
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- CN110432294A CN110432294A CN201910788485.8A CN201910788485A CN110432294A CN 110432294 A CN110432294 A CN 110432294A CN 201910788485 A CN201910788485 A CN 201910788485A CN 110432294 A CN110432294 A CN 110432294A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
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Abstract
For the bacteriostatic agent of biofermentation provided by the invention using food materials as raw material, raw material sources are abundant；It adds probiotic composition and produces the residue of ecological bean curd, by way of fermentation, antibacterial effective substance sufficiently in release raw material, it is simultaneously the small-molecule substance and inorganic salts being easily absorbed and used by plants by the substance decomposition of the macromolecular in raw material, the growth and breeding of harmful bacteria can effectively be inhibited, while small-molecule substance therein and inorganic salts can provide growth required nutrient as bio-fertilizer for crops.The bacteriostatic agent of biofermentation provided by the invention is to obtain using pure plant as raw material through everfermentation, does not add chemical substance, nontoxic, harmful bacteria is not likely to produce drug resistance, can improve environment, balances microorganism in environment, does not increase environmental pressure, raw material sources are abundant, at low cost, and fungistatic effect is prominent.
Description
Technical field
The invention belongs to bacteriostatic agent fields, and in particular to a kind of bacteriostatic agent of biofermentation, preparation method and application.
Background technique
The micro organism quantity of nature is huge, and extensively, some microorganisms and plant have synbiosis, to plant for classification
It is beneficial bacterium, such as nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium bacterium etc. that growth, which has beneficial microorganism, and some microorganisms are then to plant
The growth of object is harmful, is harmful microorganism, such as various germs.Harmful bacteria can generate unfavorable shadow to the growth of crops
It rings, first is that diseased region is easy to cause to have phenomena such as rotten, spot, withered, ulcer, second is that crop is by the harmful bacteria later period,
Diseased region has bacterial ooze spilling, and harm is caused to expand.Bacteriosis symptom mainly has: rotting, due to the pectase of bacterial secretory
Decomposition and the plump succulence organ such as make root, stem, root tuber, stem tuber, fruit, the fringe of aggrieved plant cell dissociation, tissue
Collapse is rotted, such as soft rot of cabbage.Necrosis occurs mainly on blade and stem, a variety of different spots or dried-up occurs, preceding
Person's such as cotton angular leaf spot, the latter's such as bacterial blight of rice.It wilts, because bacterium colonizes in vascular bundle obstruction conduit or because of bacterial poison
Element and cause, such as bacterial wilt.Tumour makes host cell hyperplasia, tissue expand and be formed, such as canker since bacterium stimulates.It is yellow
Change is stunt, and so that plant is showed yellow, atrophy in the bacterium of xylem parasitism, as grape Pi Ershi disease, apricot leaf scorch, clover are downgraded
Disease, sugarcane dwarfing and peach young fruit disease etc..
In order to inhibit harm of the harmful microorganism to crops, agricultural bacteriostatic agent comes into being, but in the prior art
The mode sprayed insecticide is generallyd use for the phenomenon that diseases and pests of agronomic crop and harms of microbe to be alleviated, but existing skill
Pesticide in art is usually that chemically synthesized pesticide, one side disease pest and microbes generate anti-medicine to chemically synthesized pesticide
Property, therefore lead to the failure of pesticide, on the other hand chemically synthesized pesticide is usually not easily decomposed, can be in crops or soil
Residual, harmful to human or is environment.
Summary of the invention
For the technical problem more than solving, the present invention provide it is a kind of using pure natural material as raw material, by repeatedly sending out
The method of ferment obtains the safe and non-toxic bacteriostatic agent for having effective inhibiting effect to harmful bacteria.
The object of the present invention is to provide a kind of bacteriostatic agents of biofermentation.
It is a further object of the present invention to provide a kind of preparation methods of above-mentioned biofermentation bacteriostatic agent.
There is provided the application of above-mentioned biofermentation bacteriostatic agent to another mesh of the invention.
Purpose according to the present invention, biofermentation bacteriostatic agent provided by the invention, the raw material including following parts by weight:
3-6 parts of garlic, 3-6 parts of onion, 3-6 parts of balsam pear, 3-6 parts of lemon, the first 0.8-1.2 parts of probiotic composition and beans
18-22 parts of water；
First probiotic composition includes: 12-18 parts of bacillus laterosporus according to parts by weight, Su Yun bacterium bacillus 18-
22 parts, 18-22 parts of saccharomycete, 12-18 parts of bacillus licheniformis, 20-30 parts of bacillus subtilis, 3-6 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 8-12 parts of soybean, the second probiotic composition 0.5-
0.8 part, 85-95 parts of water；
Second probiotic composition includes: 12-18 parts of acidophilus streptococcus according to parts by weight, lactobacillus bulgaricus
12-18 parts, 18-25 parts of bifidobacterium bifidum, 18-25 parts of lactobacillus acidophilus, 25-35 parts of Lactobacillus casei.
For the bacteriostatic agent of biofermentation provided by the invention using pure plant as raw material, garlic, onion have strong impulse
Property smell, contain citric acid containing substances, lemons such as Charantin, a variety of amino acid, have strong aromatic odor in balsam pear, contain
Vitamin C abundant, containing there are many nutriment in beans water, containing there are many probiotics in the first probiotic composition, on the one hand
Through everfermentation, the probiotics in the first probiotic composition can decompose the substance in raw material, on the other hand, by hair
After ferment, the first probiotics passes through growth and breeding, contains a large amount of probiotics in bacteriostatic agent.Raw material after everfermentation, wherein
Antibacterial substance adequately discharged, during being contacted with harmful bacteria, harmful bacteria can be killed effectively, thus
Reach the effect of inhibiting crops harmful bacteria, includes wherein furthermore a variety of probiotics and nutriment, benefit can be absorbed by plants
With promoting the quality and yield of crops.
Biofermentation bacteriostatic agent provided by the invention adds the residue and benefit for producing ecological bean curd using plant as raw material
Raw bacterium mixture, obtained bacteriostatic agent have no toxic side effect, and do not increase environmental pressure, and raw material sources are extensive, at low cost, and harmful bacteria
It is not likely to produce drug resistance.
Preferably, the bacteriostatic agent of the biofermentation, the raw material including following parts by weight:
5 parts of garlic, 5 parts of onion, 5 parts of balsam pear, 5 parts of lemon, the first 1 part of probiotic composition and 20 parts of beans water；
First probiotic composition includes: 12-18 parts of bacillus laterosporus according to parts by weight, Su Yun bacterium bacillus 18-
22 parts, 18-22 parts of saccharomycete, 12-18 parts of bacillus licheniformis, 20-30 parts of bacillus subtilis, 3-6 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 8-12 parts of soybean, the second probiotic composition 0.5-
0.8 part, 85-95 parts of water；
Second probiotic composition includes: 12-18 parts of acidophilus streptococcus according to parts by weight, lactobacillus bulgaricus
12-18 parts, 18-25 parts of bifidobacterium bifidum, 18-25 parts of lactobacillus acidophilus, 25-35 parts of Lactobacillus casei.
Preferably, the bacteriostatic agent of above-mentioned biofermentation, the raw material including following parts by weight:
5 parts of garlic, 5 parts of onion, 5 parts of balsam pear, 5 parts of lemon, the first 1 part of probiotic composition and 20 parts of beans water；
First probiotic composition includes: 15 parts of bacillus laterosporus according to parts by weight, 20 parts of Su Yun bacterium bacillus, ferment
20 parts of female bacterium, 15 parts of bacillus licheniformis, 25 parts of bacillus subtilis, 5 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 10 parts of soybean, the second 0.6 part of probiotic composition,
90 parts of water；
Second probiotic composition includes: 15 parts of acidophilus streptococcus according to parts by weight, and 15 parts of lactobacillus bulgaricus,
20 parts of bifidobacterium bifidum, 20 parts of lactobacillus acidophilus, 30 parts of Lactobacillus casei.
The preparation method of the bacteriostatic agent of above-mentioned biofermentation, comprising the following steps:
(1) beans water is prepared:
A. soybean is added to the water immersion, is beaten, obtains soya-bean milk；
B. soya-bean milk step A obtained passes through the fine defibrination of 60-120 mesh, and boils, and obtains ripe soya-bean milk；
C. the ripe soya-bean milk in step B is cooled to 80-90 DEG C, kept the temperature, and the second probiotic composition is at the uniform velocity added to
In the ripe soya-bean milk of step B, continues stirring until soya-bean milk and be frozen into bean curd, take upper liquid；
D. upper liquid obtained in step C is filtered, takes clarified solution, obtains the beans water；
(2) garlic, onion, balsam pear and lemon are cleaned up, is then added in the beans water that step (1) obtains, mixed
Uniformly, it is beaten, obtains mixing underflow；
(3) the mixing underflow for obtaining step (2) sterilizes, and obtains sterilizing mixing underflow；
(4) sterilizing that step (3) obtains is mixed underflow to be added in disinfection fermentation tank, it is mixed that the first probiotics is then added
Object is closed, is sealed by fermentation, obtains the bacteriostatic agent of the biofermentation.
Preparation method provided by the invention carries out preparing beans water first, during carrying out preparing ecological bean curd, pass through
Nutriment in the method dissociation soybean of fermentation, antibacterial substance is contained in remaining supernatant, by by residue and former
The method for expecting to ferment, the nutriment in raw material is adequately dissociated, and effective bacteriostatic ingredients are adequately discharged, other
Nutriment be broken down into small molecule, easily absorbed by plant.Method provided by the invention, easy to operate, what is obtained is antibacterial
Agent has containing a large amount of effective bacteriostatic ingredients and microelement, beneficial flora etc., while antibacterial, can provide for crops
Nutrition.
Preferably, in step (1), the time impregnated in step A is 8-10h.By immersion in 8-10 hours, soybean could
Moisture is adequately absorbed, the smoothly progress of defibrination is conducive to, does sufficient preparation for subsequent fine fine grinding slurry.
Preferably, in step (1), in step B, by the soya-bean milk that step A is obtained successively pass through 60 mesh, 80 mesh, 100 mesh and
The 120 fine defibrinations of mesh four times.The present invention passes through 4 fine defibrinations, and soybean can adequately be ground, and obtained soya-bean milk is fine and smooth
Uniformly.
Preferably, in step (3), the temperature of the sterilizing is 110-120 DEG C, and the time of sterilizing is 5-20min.
Preferably, in step (4), the temperature of the fermentation is 35-40 DEG C, and the time of fermentation is 48-72h.
The bacteriostatic agent of above-mentioned biofermentation can be applied in prevention and treatment crops harmful bacteria.Biology hair provided by the invention
Containing the harmful bacteria that there are many antibacterial substance, can effectively inhibit parasitic attachment on crops in the bacteriostatic agent of ferment, harmful bacteria is killed,
Reduce the pest and disease damage of crops.
The bacteriostatic agent of above-mentioned biofermentation can be applied in crop foliage fertilizer.Biofermentation provided by the invention
In bacteriostatic agent, further includes a variety of probiotics and nutriment other than containing effective bacteriostatic ingredients, foliar fertilizer can be used as, directly
It is sprayed at the blade face of crops, supplements the nutrients for crops, improves the quality and yield of crops.
The invention has the benefit that
1. the bacteriostatic agent of biofermentation provided by the invention, using food materials as raw material, raw material sources are abundant；It is mixed to add probiotics
It closes object and produces the residue of ecological bean curd, by way of fermentation, sufficiently discharge the antibacterial effective substance in raw material, while will be former
The substance decomposition of macromolecular in material is the small-molecule substance and inorganic salts being easily absorbed and used by plants, and can effectively inhibit to have
The growth and breeding of evil bacterium, while small-molecule substance therein and inorganic salts can provide growth as bio-fertilizer for crops
Required nutrient.
2. the bacteriostatic agent of biofermentation provided by the invention is obtained through everfermentation, not addition using pure plant as raw material
Article is learned, nontoxic, harmful bacteria is not likely to produce drug resistance, can improve environment, balances microorganism in environment, and it is negative not increase environment
Load, raw material sources are abundant, at low cost.What the bacteriostatic agent of biofermentation provided by the invention can give full play in raw material antibacterial has
Ingredient is imitated, fungistatic effect is prominent.
3. biofermentation bacteriostatic agent provided by the invention is raw material using the ecological remaining beans water of bean curd is produced, preparation is realized
The recycling of resource in soybean curd production process reduces by soybean curd production process 90% or more water consumption problem, solves bean curd
The waste water generated in production process realizes sewage zero-discharge, turns waste into wealth, reduce cost.
4. probiotics rich in biofermentation bacteriostatic agent provided by the invention, be able to suppress crop surface has
The growth of evil bacterium, while there are also microelement abundant and amino acid in the bacteriostatic agent obtained through everfermentation, provide for crops
Effective nutritional ingredient facilitates the increasing both production and income of crops, promotes the quality of crops.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
Every 1 parts by weight correspond to 10g in following embodiment.
Embodiment 1
A kind of bacteriostatic agent of biofermentation, the raw material including following parts by weight:
3 parts of garlic, 3 parts of onion, 3 parts of balsam pear, 3 parts of lemon, the first 0.8 part of probiotic composition and 18 parts of beans water；
First probiotic composition includes: 12 parts of bacillus laterosporus according to parts by weight, 18 parts of Su Yun bacterium bacillus, ferment
18 parts of female bacterium, 12 parts of bacillus licheniformis, 20 parts of bacillus subtilis, 3 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 8 parts of soybean, the second 0.5 part of probiotic composition, and water
95 parts；
Second probiotic composition includes: 12 parts of acidophilus streptococcus according to parts by weight, and 12 parts of lactobacillus bulgaricus,
18 parts of bifidobacterium bifidum, 18 parts of lactobacillus acidophilus, 25 parts of Lactobacillus casei.
The preparation method of the bacteriostatic agent of above-mentioned biofermentation, comprising the following steps:
A. soybean is added to the water and impregnates 8h, mashing obtains soya-bean milk；
B. the soya-bean milk that step A is obtained successively is passed through into 4 60 mesh, 80 mesh, 100 mesh and 120 mesh fine defibrinations, and boiled,
Obtain ripe soya-bean milk；
C. the ripe soya-bean milk in step B is cooled to 80-90 DEG C, kept the temperature, and the second probiotic composition is at the uniform velocity added to
In the ripe soya-bean milk of step B, continues stirring until soya-bean milk and be frozen into bean curd, take upper liquid；
D. upper liquid obtained in step C is filtered, takes clarified solution, obtains the beans water；
(2) garlic, onion, balsam pear and lemon are cleaned up, is then added in the beans water that step (1) obtains, mixed
Uniformly, it is beaten, obtains mixing underflow；
(3) the mixing underflow for obtaining step (2) obtains sterilizing mixing underflow in 110 DEG C of sterilizing 20min；
(4) sterilizing that step (3) obtains is mixed underflow to be added in disinfection fermentation tank, it is mixed that the first probiotics is then added
It closes object and obtains the bacteriostatic agent of the biofermentation in 35-37 DEG C of sealing and fermenting 72h.
Embodiment 2
A kind of bacteriostatic agent of biofermentation, the raw material including following parts by weight:
6 parts of garlic, 6 parts of onion, 6 parts of balsam pear, 6 parts of lemon, the first 1.2 parts of probiotic composition and 22 parts of beans water；
First probiotic composition includes: 18 parts of bacillus laterosporus according to parts by weight, 22 parts of Su Yun bacterium bacillus, ferment
22 parts of female bacterium, 18 parts of bacillus licheniformis, 30 parts of bacillus subtilis, 6 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 12 parts of soybean, the second 0.8 part of probiotic composition,
85 parts of water；
Second probiotic composition includes: 18 parts of acidophilus streptococcus according to parts by weight, and 18 parts of lactobacillus bulgaricus,
25 parts of bifidobacterium bifidum, 25 parts of lactobacillus acidophilus, 35 parts of Lactobacillus casei.
The preparation method of the bacteriostatic agent of above-mentioned biofermentation, comprising the following steps:
(1) beans water is prepared:
A. soybean is added to the water and impregnates 10h, mashing obtains soya-bean milk；
B. the soya-bean milk that step A is obtained successively is passed through into 4 60 mesh, 80 mesh, 100 mesh and 120 mesh fine defibrinations, and boiled,
Obtain ripe soya-bean milk；
C. the ripe soya-bean milk in step B is cooled to 80-90 DEG C, kept the temperature, and the second probiotic composition is at the uniform velocity added to
In the ripe soya-bean milk of step B, continues stirring until soya-bean milk and be frozen into bean curd, take upper liquid；
D. upper liquid obtained in step C is filtered, takes clarified solution, obtains the beans water；
(2) garlic, onion, balsam pear and lemon are cleaned up, is then added in the beans water that step (1) obtains, mixed
Uniformly, it is beaten, obtains mixing underflow；
(3) the mixing underflow for obtaining step (2) obtains sterilizing mixing underflow in 120 DEG C of sterilizing 5min；
(4) sterilizing that step (3) obtains is mixed underflow to be added in disinfection fermentation tank, it is mixed that the first probiotics is then added
It closes object and obtains the bacteriostatic agent of the biofermentation in 38-40 DEG C of sealing and fermenting 48h.
Embodiment 3
A kind of bacteriostatic agent of biofermentation, the raw material including following parts by weight:
5 parts of garlic, 5 parts of onion, 5 parts of balsam pear, 5 parts of lemon, the first 1 part of probiotic composition and 20 parts of beans water；
First probiotic composition includes: 12 parts of bacillus laterosporus according to parts by weight, 18 parts of Su Yun bacterium bacillus, ferment
18 parts of female bacterium, 12 parts of bacillus licheniformis, 20 parts of bacillus subtilis, 3 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 8 parts of soybean, the second 0.5 part of probiotic composition, and water
95 parts；
Second probiotic composition includes: 12 parts of acidophilus streptococcus according to parts by weight, and 12 parts of lactobacillus bulgaricus,
18 parts of bifidobacterium bifidum, 18 parts of lactobacillus acidophilus, 25 parts of Lactobacillus casei.
The preparation method of the bacteriostatic agent of above-mentioned biofermentation, comprising the following steps:
(1) beans water is prepared:
A. soybean is added to the water and impregnates 8h, mashing obtains soya-bean milk；
B. the soya-bean milk that step A is obtained successively is passed through into 4 60 mesh, 80 mesh, 100 mesh and 120 mesh fine defibrinations, and boiled,
Obtain ripe soya-bean milk；
C. the ripe soya-bean milk in step B is cooled to 80-90 DEG C, kept the temperature, and the second probiotic composition is at the uniform velocity added to
In the ripe soya-bean milk of step B, continues stirring until soya-bean milk and be frozen into bean curd, take upper liquid；
D. upper liquid obtained in step C is filtered, takes clarified solution, obtains the beans water；
(2) garlic, onion, balsam pear and lemon are cleaned up, is then added in the beans water that step (1) obtains, mixed
Uniformly, it is beaten, obtains mixing underflow；
(3) the mixing underflow for obtaining step (2) obtains sterilizing mixing underflow in 115 DEG C of sterilizing 15min；
(4) sterilizing that step (3) obtains is mixed underflow to be added in disinfection fermentation tank, it is mixed that the first probiotics is then added
It closes object and obtains the bacteriostatic agent of the biofermentation in 36-38 DEG C of sealing and fermenting 60h.
Embodiment 4
A kind of bacteriostatic agent of biofermentation, the raw material including following parts by weight:
5 parts of garlic, 5 parts of onion, 5 parts of balsam pear, 5 parts of lemon, the first 1 part of probiotic composition and 20 parts of beans water；
First probiotic composition includes: 18 parts of bacillus laterosporus according to parts by weight, 22 parts of Su Yun bacterium bacillus, ferment
22 parts of female bacterium, 18 parts of bacillus licheniformis, 30 parts of bacillus subtilis, 6 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 12 parts of soybean, the second 0.8 part of probiotic composition,
85 parts of water；
Second probiotic composition includes: 18 parts of acidophilus streptococcus according to parts by weight, and 18 parts of lactobacillus bulgaricus,
25 parts of bifidobacterium bifidum, 25 parts of lactobacillus acidophilus, 35 parts of Lactobacillus casei.
The preparation method of the bacteriostatic agent of above-mentioned biofermentation is same as Example 3.
Embodiment 5
A kind of bacteriostatic agent of biofermentation, the raw material including following parts by weight:
5 parts of garlic, 5 parts of onion, 5 parts of balsam pear, 5 parts of lemon, the first 1 part of probiotic composition and 20 parts of beans water；
First probiotic composition includes: 15 parts of bacillus laterosporus according to parts by weight, 20 parts of Su Yun bacterium bacillus, ferment
20 parts of female bacterium, 15 parts of bacillus licheniformis, 25 parts of bacillus subtilis, 5 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 10 parts of soybean, the second 0.6 part of probiotic composition,
90 parts of water；
Second probiotic composition includes: 15 parts of acidophilus streptococcus according to parts by weight, and 15 parts of lactobacillus bulgaricus,
20 parts of bifidobacterium bifidum, 20 parts of lactobacillus acidophilus, 30 parts of Lactobacillus casei.
The preparation method of the bacteriostatic agent of above-mentioned biofermentation is same as Example 3.
Comparative example 1
A kind of bacteriostatic agent of biofermentation, the raw material including following parts by weight:
1 part of garlic, 1 part of onion, 1 part of balsam pear, 7 parts of lemon, 1 part and 20 parts of water of saccharomycete.
The preparation method of the bacteriostatic agent of above-mentioned biofermentation, comprising the following steps:
(1) garlic, onion, balsam pear and lemon clean up, and are uniformly mixed, and water is added, and mashing obtains mixing underflow；
(2) the mixing underflow for obtaining step (1) obtains sterilizing mixing underflow in 115 DEG C of sterilizing 15min；
(3) sterilizing that step (2) obtains is mixed underflow to be added in disinfection fermentation tank, saccharomycete is then added, in 36-
38 DEG C of sealing and fermenting 60h, obtain the bacteriostatic agent of the biofermentation.
Test example
1. bacteriostatic test
Wheat bacterial spike blight bacterium and pseudomonas syringae are taken, is activated, test strain is obtained.Then three groups of MH meat soups are taken
Culture medium, every group of culture medium are 10, and first group is test group, and test group is divided into Liang Ge group, 5 culture mediums of each group,
First group is inoculated with wheat bacterial spike blight bacterium group, and the second group is inoculated with pseudomonas syringae；Second group is control group, compares component
For Liang Ge group, 5 culture mediums of each group, the first group is inoculated with wheat bacterial spike blight bacterium group, and it is false single that the second group is inoculated with cloves
Born of the same parents bacillus；Third group is blank group, and blank elements are Liang Ge group, and 5 culture mediums of each group, it is sweet that the first group is inoculated with wheat
Fringe germ group, the second group are inoculated with pseudomonas syringae.The strain of identical quantity, every bacterium are inoculated with after each medium sterilization
Number is about 104CFU.The biofermentation bacteriostatic agent for the sterilizing that embodiment 5 obtains is added dropwise in test group, and control group is added dropwise comparative example 1 and obtains
The biofermentation bacteriostatic agent for the sterilizing arrived, blank group are not processed.
The isoperibol that culture medium is placed on 35-37 DEG C is cultivated for 24 hours, the culture solution in each culture medium is then taken
10 μ L, are then seeded to Solid agar culture, cultivate at 35-37 DEG C for 24 hours, and bacterium colony on observation statistics culture medium the results are shown in Table
1。
Bacteriostasis rate=(average colony number that every group of the average colony number-of blank group)/blank group average colony number ×
100%
1 bacteriostatic test result of table
Wheat bacterial spike blight bacterium is gram-positive bacteria, and wheat bacterial spike blight bacterium is propagated by nematode, harmful to the growth of wheat
Effect, pseudomonas syringae is Gram-negative bacteria, it can endanger various plants, including cereal crops, vegetables, fruit tree, flower
Grass and medicinal material.From the results shown in Table 1, the bacteriostatic agent of biofermentation provided by the invention, experimental group is to wheat bacterial spike blight
Bacterium has 99% inhibiting effect, there is 98% inhibiting effect to pseudomonas syringae；Contrast groups have wheat bacterial spike blight bacterium
70% inhibiting effect has 76% inhibiting effect to pseudomonas syringae, and effect is slightly worse, illustrates the bacteriostatic agent of biofermentation
Raw material used itself has certain sterilization effect, but match and fermentation so that the fungistatic effect of the bacteriostatic agent of biofermentation more
For protrusion.
2. fertility promotes effect
Subjects: rice, corn and soybean
Test method: Example 5, comparative example 1 and clear water are respectively as test group, contrast groups and blank group；Take three pieces
Paddy field, every plot of paddy field are 1 mu；The preparation of test group, control group and blank group is respectively adopted before Rice Cropping to leaf
Face is sprayed, average to spray 5kg per acre；During Rice Cropping, three groups of preparations of sprinkling in 30 days that are averaged, every time
1-2kg is sprayed per acre, is waited rice mature, is counted the yield of three groups of rice.
Wherein the test of corn and soybean is identical with rice, counts three kinds of yield being used as, the results are shown in Table 2.
The output statistics result of 2 three kinds of crops of table
From the results shown in Table 2: the bacteriostatic agent of biofermentation provided by the invention gets antibacterial disease resistance to crop
Effect, crops thrive, and improve yield, not only get protective effect to crop after bacteriostatic agent sprinkling, enrich
Beneficial bacterium and nutrient can improve soil, increase soil fertility, the growth and development of useful crop plants.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of bacteriostatic agent of biofermentation, which is characterized in that the raw material including following parts by weight:
3-6 parts of garlic, 3-6 parts of onion, 3-6 parts of balsam pear, 3-6 parts of lemon, the first 0.8-1.2 parts of probiotic composition and beans water
18-22 parts；
First probiotic composition includes: 12-18 parts of bacillus laterosporus according to parts by weight, 18-22 parts of Su Yun bacterium bacillus,
18-22 parts of saccharomycete, 12-18 parts of bacillus licheniformis, 20-30 parts of bacillus subtilis, 3-6 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 8-12 parts of soybean, the second probiotic composition 0.5-0.8
Part, 85-95 parts of water；
Second probiotic composition includes: 12-18 parts of acidophilus streptococcus according to parts by weight, lactobacillus bulgaricus 12-18
Part, 18-25 parts of bifidobacterium bifidum, 18-25 parts of lactobacillus acidophilus, 25-35 parts of Lactobacillus casei.
2. the bacteriostatic agent of biofermentation according to claim 1, which is characterized in that the raw material including following parts by weight:
5 parts of garlic, 5 parts of onion, 5 parts of balsam pear, 5 parts of lemon, the first 1 part of probiotic composition and 20 parts of beans water；
First probiotic composition includes: 12-18 parts of bacillus laterosporus according to parts by weight, 18-22 parts of Su Yun bacterium bacillus,
18-22 parts of saccharomycete, 12-18 parts of bacillus licheniformis, 20-30 parts of bacillus subtilis, 3-6 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 8-12 parts of soybean, the second probiotic composition 0.5-0.8
Part, 85-95 parts of water；
Second probiotic composition includes: 12-18 parts of acidophilus streptococcus according to parts by weight, lactobacillus bulgaricus 12-18
Part, 18-25 parts of bifidobacterium bifidum, 18-25 parts of lactobacillus acidophilus, 25-35 parts of Lactobacillus casei.
3. the bacteriostatic agent of biofermentation according to claim 2, which is characterized in that the raw material including following parts by weight:
5 parts of garlic, 5 parts of onion, 5 parts of balsam pear, 5 parts of lemon, the first 1 part of probiotic composition and 20 parts of beans water；
First probiotic composition includes: 15 parts of bacillus laterosporus according to parts by weight, 20 parts of Su Yun bacterium bacillus, saccharomycete
20 parts, 15 parts of bacillus licheniformis, 25 parts of bacillus subtilis, 5 parts of Thiobacillus thioxidans；
The beans water includes that the raw material of following parts by weight is prepared: 10 parts of soybean, the second 0.6 part of probiotic composition, and water 90
Part；
Second probiotic composition includes: 15 parts of acidophilus streptococcus according to parts by weight, 15 parts of lactobacillus bulgaricus, not tally
20 parts of Bifidobacterium, 20 parts of lactobacillus acidophilus, 30 parts of Lactobacillus casei.
4. the preparation method of the bacteriostatic agent of any biofermentation of claim 1-3, which is characterized in that including following step
It is rapid:
(1) beans water is prepared:
A. soybean is added to the water immersion, is beaten, obtains soya-bean milk；
B. soya-bean milk step A obtained passes through the fine defibrination of 60-120 mesh, and boils, and obtains ripe soya-bean milk；
C. the ripe soya-bean milk in step B is cooled to 80-90 DEG C, kept the temperature, and the second probiotic composition is at the uniform velocity added to step B
Ripe soya-bean milk in, continue stirring until soya-bean milk and be frozen into bean curd, take upper liquid；
D. upper liquid obtained in step C is filtered, takes clarified solution, obtains the beans water；
(2) garlic, onion, balsam pear and lemon are cleaned up, are then added in the beans water that step (1) obtains, is uniformly mixed,
Mashing obtains mixing underflow；
(3) the mixing underflow for obtaining step (2) sterilizes, and obtains sterilizing mixing underflow；
(4) sterilizing that step (3) obtains is mixed underflow to be added in disinfection fermentation tank, the mixing of the first probiotics is then added
Object is sealed by fermentation, obtains the bacteriostatic agent of the biofermentation.
5. the preparation method of the bacteriostatic agent of biofermentation according to claim 4, which is characterized in that in step (1), step
The time impregnated in A is 8-10h.
6. the preparation method of the bacteriostatic agent of biofermentation according to claim 4, which is characterized in that in step (1), step
In B, the soya-bean milk that step A is obtained successively is passed through into four 60 mesh, 80 mesh, 100 mesh and 120 mesh fine defibrinations.
7. the preparation method of the bacteriostatic agent of biofermentation according to claim 4, which is characterized in that described in step (3)
The temperature of sterilizing is 110-120 DEG C, and the time of sterilizing is 5-20min.
8. the preparation method of the bacteriostatic agent of biofermentation according to claim 4, which is characterized in that described in step (4)
The temperature of fermentation is 35-40 DEG C, and the time of fermentation is 48-72h.
9. application of the bacteriostatic agent of any biofermentation of claim 1-3 in prevention and treatment crops harmful bacteria.
10. application of the bacteriostatic agent of any biofermentation of claim 1-3 in crop foliage fertilizer.
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| CN110800840A (en) * | 2019-11-15 | 2020-02-18 | 黑龙江惊哲森林食品集团有限公司 | Soybean water lactobacillus ferment beverage and preparation method thereof |
| CN111729914A (en) * | 2020-06-08 | 2020-10-02 | 山东惊哲生物科技有限公司 | Probiotic bacteria composition for degrading landscaping waste and preparation method thereof |
| CN116686903A (en) * | 2023-08-03 | 2023-09-05 | 山东健源生物科技有限公司 | Complex bacterial enzyme biological starter and application thereof |
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| CN116420741B (en) * | 2023-03-21 | 2024-01-23 | 吉林桑黄生物科技集团有限公司 | Composite biological agent for mulberry Huang Chongzhi and planting method of high-yield phellinus linteus |
| CN116686903A (en) * | 2023-08-03 | 2023-09-05 | 山东健源生物科技有限公司 | Complex bacterial enzyme biological starter and application thereof |
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Application publication date: 20191112 |