CN109776217A - A kind of biological bacteria composite foliage fertilizer and its preparation method and application - Google Patents
A kind of biological bacteria composite foliage fertilizer and its preparation method and application Download PDFInfo
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- CN109776217A CN109776217A CN201910217267.9A CN201910217267A CN109776217A CN 109776217 A CN109776217 A CN 109776217A CN 201910217267 A CN201910217267 A CN 201910217267A CN 109776217 A CN109776217 A CN 109776217A
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- bacterium solution
- bifidobacterium
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- trichoderma
- bacillus subtilis
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Abstract
The present invention relates to bio-fertilizer technical fields more particularly to a kind of biological bacteria composite foliage fertilizer and its preparation method and application.The biological bacteria composite foliage fertilizer includes the raw material of following parts by weight: sea grass dry powder, composite bacteria agent, molasses, potassium dihydrogen phosphate；The composite bacteria agent is bacillus subtilis, Bifidobacterium, Trichoderma and lactobacillus plantarum.The biological bacteria composite foliage fertilizer preferably promotes crops precocity, improves crop yield.
Description
Technical field
The present invention relates to bio-fertilizer technical field more particularly to a kind of biological bacteria composite foliage fertilizer and preparation method thereof and answer
With.
Background technique
Foliar fertilizer is that nutrient needed for crop is directly applied to the fertilizer on blade face for the purpose of Foliage Absorption.Foliar fertilizer is battalion
Feeding element is applied to crops blade surface, and a kind of fertilizer type of its function, the leaf of plant are played by the absorption of blade
Piece has upper layer and lower layer epidermis, is made of epidermal cell, there is cuticula and wax on table on the outside of cell, can protect epidermal tissue
Under mesophyll cell exercise the functions such as photosynthetic, breathing, do not influenced by the variation of extraneous unfavorable conditions, blade surface is there are many more micro-
Small stomata exercises the function of gas replacement.
Currently, have many foliar fertilizers for being used for foliage spray on the market, and it is various in style, it is vdiverse in function, but effect is different；It is main
Be existing foliar fertilizer be mostly it is inorganic, utilization rate is low, in foliar fertilizer ingredient greatly can not be absorbed, cause greatly
Waste, and cause crop disease to take place frequently due to excessive use chemical fertilizer, disease takes place frequently and causes making for pesticide and fertilizer
With the harm such as excessive.
Summary of the invention
The present invention provides a kind of biological bacteria composite foliage fertilizers and its preparation method and application, solve and deposit in the prior art
The problem of.
Used technical solution is the present invention to solve above-mentioned technical problem:
A kind of biological bacteria composite foliage fertilizer, the raw material including following parts by weight: 5-20 parts of sea grass dry powder, composite bacteria agent 5-
15 parts, 5-15 parts of molasses, 1-5 parts of potassium dihydrogen phosphate；
The composite bacteria agent is bacillus subtilis, Bifidobacterium, trichoderma and lactobacillus plantarum.
The composite bacteria agent is bacillus subtilis bacterium solution, Bifidobacterium bacterium solution, Trichoderma bacterium solution and lactobacillus plantarum bacterium
Liquid；Each bacterium solution presses bacillus subtilis bacterium solution: Trichoderma bacterium solution: lactobacillus plantarum bacterium solution: Bifidobacterium bacterium solution=6:3:4:5
Weight ratio cooperation.
Each bacterial concentration is bacillus subtilis 4*107-1*1010Cfu/mL, Bifidobacterium 4*108-1*109Cfu/mL, wood
Mould 1*102-6*103Cfu/mL, lactobacillus plantarum 1*103-6*105cfu/mL。
Each bacterial concentration is bacillus subtilis 1*109-10*109Cfu/mL, Bifidobacterium 6*108-1*109cfu/mL、
Trichoderma 3*103-6*103Cfu/mL, lactobacillus plantarum 4*104-6*104cfu/mL。
The bacillus subtilis, Bifidobacterium, Trichoderma and lactobacillus plantarum are commercially available active bacteria formulation product.
The biological bacteria composite foliage fertilizer, the raw material including following parts by weight: 12 parts of sea grass dry powder, composite bacteria agent 10
Part, 10 parts of molasses, 3 parts of potassium dihydrogen phosphate.
The preparation method of above-mentioned biological bacteria composite foliage fertilizer, including following operating procedure:
(1) bacillus subtilis, Bifidobacterium, trichoderma and lactobacillus plantarum are taken respectively, are activated, are obtained each bacterium bacterium solution, it is spare；
(2) above-mentioned parts by weight are pressed, step (1) is added by several times and removes bifid for mixing sea grass dry powder, molasses and potassium dihydrogen phosphate
Half-dried fermentation material, fermented and cultured certain time is made in other bacterium solutions except bacillus bacterium solution；Then be added Bifidobacterium bacterium solution after
Supervention ferment certain time obtains fermentation liquid to get the biological bacteria composite foliage fertilizer.
The preparation method further includes the steps that the fermentation liquid to step (2) carries out being concentrated and dried to obtain powder.
The activation act of each bacterium in step (1) are as follows:
Bacillus subtilis activation: taking 120-500g brown sugar, warm water 10L, is kept for 25-37 DEG C of temperature, by 1Kg withered grass bud
Spore bacillus bacterium powder is added in the brown sugar water dissolved, activates 3-5h；Bacterium solution is diluted with water to above-mentioned bacillus subtilis bacterium solution
Concentration；
Trichoderma activation: Trichoderma is diluted with water to above-mentioned Trichoderma bacterial concentration；
Lactobacillus plantarum activation: lactobacillus plantarum bacterium solution is activated to obtain using lactobacillus plantarum culture medium, is diluted to above-mentioned plant
Object lactobacillus bacterial concentration；
Bifidobacterium activation: Bifidobacterium is activated using MRS agar medium, Bifidobacterium bacterium solution is obtained, is diluted to above-mentioned
Bifidobacterium bacterial concentration；
Fermented and cultured step in step (2) are as follows: based on above-mentioned parts by weight, first by bacillus subtilis bacterium solution, Trichoderma
Bacterium solution and lactobacillus plantarum bacterium solution are sequentially added into uniformly mixed sea grass dry powder, molasses and potassium dihydrogen phosphate, are made half-dried
Fermentation material, in 30-36 DEG C of aerobic fermentation 24-72h；Then 36-43 DEG C of fermentation temperature is controlled, Bifidobacterium bacterium solution is added, is continued
Anaerobic fermentation 24-36h；Obtain fermentation liquid.
The dosage form of the biological bacteria composite foliage fertilizer is aqua, suspending agent or powder formulation.
The biological bacteria composite foliage fertilizer is promoting crops precocity, is improving the application in crop yield.
The biological bacteria composite foliage fertilizer is aqua, and the amount of spraying after the biological bacteria composite foliage fertilizer aqua dilution is
45-55Kg/ mus.
The aqua is diluted by the amount of water of 1:300-400, spraying to apply.
Beneficial effects of the present invention:
The biological bacteria composite foliage fertilizer abandons the scheme that existing foliar fertilizer is mostly inorganic constituents, using sea grass dry powder, compound
Microbial inoculum, molasses, potassium dihydrogen phosphate ferment together, prepare resulting foliar fertilizer formulation products fertilizer efficiency height, preferably promote farming
Object is precocious, improves crop yield；Meanwhile composite bacteria agent is compound using four kinds of ingredient bacterium, and it is mutually synergistic, it ensure that leaf
When leaven sprays, while nutritional ingredient absorbs, disease-resistant insect pest ability is enhanced.
Specific embodiment
In order to clarify the technical characteristics of the invention, being explained in detail below by specific embodiment the present invention
It states.
Embodiment 1
The biological bacteria composite foliage fertilizer, the raw material including following parts by weight: 12 parts of sea grass dry powder, 10 parts of composite bacteria agent,
10 parts of molasses, 3 parts of potassium dihydrogen phosphate；
The composite bacteria agent is bacillus subtilis bacterium solution, Bifidobacterium bacterium solution, Trichoderma bacterium solution and lactobacillus plantarum bacterium
Liquid；Bacillus subtilis bacterium solution: Trichoderma bacterium solution: lactobacillus plantarum bacterium solution: Bifidobacterium bacterium solution=6:3:4:5 weight ratio；
The bacillus subtilis 5*109Cfu/mL, Bifidobacterium 9*108Cfu/mL, Trichoderma 5*103Cfu/mL, lactobacillus plantarum 5*
104cfu/mL。
The preparation method of above-mentioned biological bacteria composite foliage fertilizer, including following operating procedure:
(1) bacillus subtilis, Bifidobacterium, Trichoderma and lactobacillus plantarum are taken respectively, are activated, are obtained each bacterium bacterium solution, it is standby
With；
Bacillus subtilis activation: taking 120g brown sugar, warm water 10L, is kept for 28 DEG C of temperature, by 1Kg bacillus subtilis
Bacterium powder is added in the brown sugar water dissolved, activates 4h；Bacterium solution is diluted with water to above-mentioned bacillus subtilis bacterial concentration；
Trichoderma activation: Trichoderma is diluted with water to above-mentioned Trichoderma bacterial concentration；
Lactobacillus plantarum activation: lactobacillus plantarum bacterium solution is activated to obtain using lactobacillus plantarum culture medium, is diluted to above-mentioned bacterium
Liquid concentration；
Bifidobacterium activation: Bifidobacterium is activated using MRS agar medium, Bifidobacterium bacterium solution is obtained, is diluted to above-mentioned
Bacterial concentration；
Each bacterium solution weight is measured respectively by aforementioned weight proportion；
(2) based on above-mentioned composite bacteria agent parts by weight, first by bacillus subtilis bacterium solution, Trichoderma bacterium solution and plant cream bar
Bacterium bacterium solution is sequentially added into uniformly mixed sea grass dry powder, molasses and potassium dihydrogen phosphate, and half-dried fermentation material is made, and is had in 34 DEG C
Aerobe fermentation 40h；Then 42 DEG C of fermentation temperature are controlled, Bifidobacterium bacterium solution is added, continues anaerobic fermentation 30h；Obtain fermentation liquid；
(3) processing is filtered to fermentation liquid obtained by step (2), takes supernatant to get the biological bacteria composite foliage fertilizer.
The aqua is diluted by the amount of water of 1:300, spraying to apply.The amount of spraying is 45Kg/ mus.
Embodiment 2
The biological bacteria composite foliage fertilizer, the raw material including following parts by weight: 5 parts of sea grass dry powder, 5 parts of composite bacteria agent, sugar
5 parts, 1 part of potassium dihydrogen phosphate of honey；
The composite bacteria agent is bacillus subtilis bacterium solution, Bifidobacterium bacterium solution, Trichoderma bacterium solution and lactobacillus plantarum bacterium
Liquid；Bacillus subtilis bacterium solution: Trichoderma bacterium solution: lactobacillus plantarum bacterium solution: Bifidobacterium bacterium solution=6:3:4:5 weight ratio；
The bacillus subtilis 1*109Cfu/mL, Bifidobacterium 6*108Cfu/mL, Trichoderma 3*103Cfu/mL, lactobacillus plantarum 4*
104cfu/mL。
The preparation method of above-mentioned biological bacteria composite foliage fertilizer, including following operating procedure:
(1) each bacterium is taken respectively, is activated, is obtained each bacterium bacterium solution, it is spare；
Bacillus subtilis activation: taking 500g brown sugar, warm water 10L, is kept for 37 DEG C of temperature, by 1Kg bacillus subtilis
Bacterium powder is added in the brown sugar water dissolved, activates 3h；Bacterium solution is diluted with water to above-mentioned bacillus subtilis bacterial concentration；
Trichoderma activation: Trichoderma is diluted with water to above-mentioned Trichoderma bacterial concentration；
Lactobacillus plantarum activation: lactobacillus plantarum bacterium solution is activated to obtain using lactobacillus plantarum culture medium, is diluted to above-mentioned bacterium
Liquid concentration；
Bifidobacterium activation: Bifidobacterium is activated using MRS agar medium, Bifidobacterium bacterium solution is obtained, is diluted to above-mentioned
Bacterial concentration；
Each bacterium solution weight is measured respectively by aforementioned weight proportion；
(2) based on above-mentioned composite bacteria agent parts by weight, first by bacillus subtilis bacterium solution, Trichoderma bacterium solution and plant cream bar
Bacterium bacterium solution is sequentially added into uniformly mixed sea grass dry powder, molasses and potassium dihydrogen phosphate, and half-dried fermentation material is made, and is had in 35 DEG C
Aerobe fermentation 48h；Then 40 DEG C of fermentation temperature are controlled, Bifidobacterium bacterium solution is added, continues anaerobic fermentation 30h；Obtain fermentation liquid；
(3) processing is filtered to fermentation liquid obtained by step (2), takes supernatant, spray drying treatment is carried out to long clear liquid,
Obtain pulvis biological bacteria composite foliage fertilizer.
Above-mentioned pulvis volume ratio 1:400 by weight is added into water, spraying to apply, the amount of spraying is 50Kg/ mus.
Embodiment 3
The biological bacteria composite foliage fertilizer, the raw material including following parts by weight: 20 parts of sea grass dry powder, 15 parts of composite bacteria agent,
15 parts of molasses, 5 parts of potassium dihydrogen phosphate；
The composite bacteria agent is bacillus subtilis bacterium solution, Bifidobacterium bacterium solution, Trichoderma bacterium solution and lactobacillus plantarum bacterium
Liquid；Bacillus subtilis bacterium solution: Trichoderma bacterium solution: lactobacillus plantarum bacterium solution: Bifidobacterium bacterium solution=6:3:4:5 weight ratio；
The bacillus subtilis 10*109Cfu/mL, Bifidobacterium 1*109Cfu/mL, Trichoderma 6*103Cfu/mL, lactobacillus plantarum
6*104cfu/mL。
The preparation method of above-mentioned biological bacteria composite foliage fertilizer, including following operating procedure:
(1) each bacterium is taken respectively, is activated, is obtained each bacterium bacterium solution, it is spare；
Bacillus subtilis activation: taking 300g brown sugar, warm water 10L, is kept for 32 DEG C of temperature, by 1Kg bacillus subtilis
Bacterium powder is added in the brown sugar water dissolved, activates 4h；Bacterium solution is diluted with water to above-mentioned bacillus subtilis bacterial concentration；
Trichoderma activation: Trichoderma is diluted with water to above-mentioned Trichoderma bacterial concentration；
Lactobacillus plantarum activation: lactobacillus plantarum bacterium solution is activated to obtain using lactobacillus plantarum culture medium, is diluted to above-mentioned bacterium
Liquid concentration；
Bifidobacterium activation: Bifidobacterium is activated using MRS agar medium, Bifidobacterium bacterium solution is obtained, is diluted to above-mentioned
Bacterial concentration；
Each bacterium solution weight is measured by aforementioned weight proportion；
(2) based on above-mentioned composite bacteria agent parts by weight, first by bacillus subtilis bacterium solution, Trichoderma bacterium solution and plant cream bar
Bacterium bacterium solution is sequentially added into uniformly mixed sea grass dry powder, molasses and potassium dihydrogen phosphate, and half-dried fermentation material is made, and is had in 30 DEG C
Aerobe fermentation 26h；Then 43 DEG C of fermentation temperature are controlled, Bifidobacterium bacterium solution is added, continues anaerobic fermentation 36h；Obtain fermentation liquid；
(3) processing is filtered to fermentation liquid obtained by step (2), takes supernatant to get the biological bacteria composite foliage fertilizer.
The aqua is diluted by the amount of water of 1:400, spraying to apply.The amount of spraying is 55Kg/ mus.
Select Qingdao of Shandong province Huangdao District experimental field of wheat, content of organic matter 1.2-1.3%, pH value 7.6-7.8, fertilizer
Power is medium, and wheat breed is Jimai 22.Experimental plot is divided into five groups, every group 1 mu of area；External environment and growth conditions are consistent,
It sprays the foliar fertilizer period and number is consistent.It is arranged one group 1 as blank control, sprays water by amount identical with foliar fertilizer is sprayed；Setting
Spray the biological bacteria composite foliage fertilizer of the embodiment of the present invention for another group 2；The blade face that another group 3 sprays existing market purchase is set
Fertilizer-foliar spray spirit.
Rate of growth is the difference percentage more increased than theoretical yield of the actual production and theoretical yield of experimental field of wheat in table
Number.
It is observed through foliage-spray under the same terms, in the entire growth cycle of wheat, compares five groups of experimental plot growing states
It was found that 2 (2.1,2.2,2.3) of group spray the experimental field of wheat crop maturity phase of the biological bacteria composite foliage fertilizer of the embodiment of the present invention
The comparative example group 1 for less spraying foliar fertilizer is advanced by 10-15 days, compared with 3 maturity period of comparative example group for spraying the existing foliar fertilizer in market
It is advanced by 5-10 days.
In terms of comprehensive yield, (2.1, the 2.2,2.3) experimental field of wheat of group 2 for spraying biological bacteria composite foliage fertilizer of the present invention increases
16-20% is produced, 3 experimental field of wheat of the group volume increase 11% of the existing foliar fertilizer in market is sprayed.
In terms of because of crop death rate caused by pest and disease damage, 2 wheat of the group test of biological bacteria composite foliage fertilizer of the present invention is sprayed
Field reduces 25% compared to 1 blank group of group, and the crop death rate for spraying existing foliar fertilizer compared to group 3 reduces 15%.
Above-mentioned specific embodiment cannot function as limiting the scope of the invention, for the technology people of the art
For member, any alternate modification or transformation made to embodiment of the present invention are fallen within the scope of protection of the present invention.
Place is not described in detail by the present invention, is the well-known technique of those skilled in the art of the present technique.
Claims (10)
1. a kind of biological bacteria composite foliage fertilizer, which is characterized in that the raw material including following parts by weight: 5-20 parts of sea grass dry powder,
5-15 parts of composite bacteria agent, 5-15 parts of molasses, 1-5 parts of potassium dihydrogen phosphate；
The composite bacteria agent is bacillus subtilis, Bifidobacterium, Trichoderma and lactobacillus plantarum.
2. biological bacteria composite foliage fertilizer according to claim 1, which is characterized in that the composite bacteria agent is bacillus subtilis
Bacterium bacterium solution, Bifidobacterium bacterium solution, Trichoderma bacterium solution and lactobacillus plantarum bacterium solution；Each bacterium solution presses bacillus subtilis bacterium solution: trichoderma
Bacterium bacterium solution: lactobacillus plantarum bacterium solution: Bifidobacterium bacterium solution=6:3:4:5 weight ratio cooperation.
3. biological bacteria composite foliage fertilizer according to claim 2, which is characterized in that each bacterial concentration is bacillus subtilis
4*107-1*1010Cfu/mL, Bifidobacterium 4*108-1*109Cfu/mL, Trichoderma 1*102-6*103Cfu/mL, lactobacillus plantarum
1*103-6*105cfu/mL。
4. biological bacteria composite foliage fertilizer according to claim 3, which is characterized in that each bacterial concentration is bacillus subtilis
1*109-10*109Cfu/mL, Bifidobacterium 6*108-1*109Cfu/mL, Trichoderma 3*103-6*103Cfu/mL, lactobacillus plantarum
4*104-6*104cfu/mL。
5. biological bacteria composite foliage fertilizer according to claim 1, which is characterized in that the raw material including following parts by weight:
12 parts of sea grass dry powder, 10 parts of composite bacteria agent, 10 parts of molasses, 3 parts of potassium dihydrogen phosphate；The composite bacteria agent is bacillus subtilis bacterium
Liquid, Bifidobacterium bacterium solution, Trichoderma bacterium solution and lactobacillus plantarum bacterium solution；Bacillus subtilis bacterium solution: Trichoderma bacterium solution: plant cream
Bacillus bacterium solution: Bifidobacterium bacterium solution=6:3:4:5 weight ratio；The bacillus subtilis 5*109Cfu/mL, Bifidobacterium 9*
108Cfu/mL, Trichoderma 5*103Cfu/mL, lactobacillus plantarum 5*104cfu/mL。
6. a kind of preparation method of biological bacteria composite foliage fertilizer as described in any one in claim 1-5, which is characterized in that including
Following operating procedure:
(1) bacillus subtilis, Bifidobacterium, Trichoderma and lactobacillus plantarum are taken respectively, are activated, are obtained each bacterium bacterium solution, it is spare；
(2) above-mentioned parts by weight are pressed, step (1) is added by several times and removes Bifidobacterium for mixing sea grass dry powder, molasses and potassium dihydrogen phosphate
Half-dried fermentation material, fermented and cultured certain time is made in other bacterium solutions except bacterium solution；Then Bifidobacterium bacterium solution is added after supervention
Ferment certain time obtains fermentation liquid to get the biological bacteria composite foliage fertilizer.
7. the preparation method of biological bacteria composite foliage fertilizer according to claim 6, which is characterized in that each bacterium in step (1)
Activation act are as follows:
Bacillus subtilis activation: taking 120-500g brown sugar, warm water 10L, is kept for 25-37 DEG C of temperature, by 1Kg bacillus subtilis
Bacterium bacterium powder is added in the brown sugar water dissolved, activates 3-5h；Bacterium solution is diluted with water to above-mentioned bacillus subtilis bacterial concentration；
Trichoderma activation: Trichoderma is diluted with water to above-mentioned Trichoderma bacterial concentration；
Lactobacillus plantarum activation: activating to obtain lactobacillus plantarum bacterium solution using lactobacillus plantarum culture medium, is diluted to above-mentioned plant cream
Bacillus bacterial concentration；
Bifidobacterium activation: Bifidobacterium is activated using MRS agar medium, Bifidobacterium bacterium solution is obtained, is diluted to above-mentioned bifid
Bacillus bacterial concentration；
Fermented and cultured step in step (2) are as follows: based on above-mentioned parts by weight, first by bacillus subtilis bacterium solution, Trichoderma bacterium solution
It sequentially adds with lactobacillus plantarum bacterium solution into uniformly mixed sea grass dry powder, molasses and potassium dihydrogen phosphate, half dry fermentation is made
Object, in 30-36 DEG C of aerobic fermentation 24-72h；Then 36-43 DEG C of fermentation temperature is controlled, Bifidobacterium bacterium solution is added, continues anaerobic
Ferment 24-36h；Obtain fermentation liquid.
8. biological bacteria composite foliage fertilizer according to claim 1-5, which is characterized in that it is characterized in that, described
Dosage form is aqua, suspending agent or powder formulation.
9. biological bacteria composite foliage fertilizer as claimed in claim 8 is promoting crops precocity, is improving answering in crop yield
With.
10. application according to claim 9, which is characterized in that the biological bacteria composite foliage fertilizer is aqua, the biology
The amount of spraying after the dilution of bacterium composite foliage fertilizer aqua is 45-55Kg/ mus.
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| CN112174718A (en) * | 2020-09-29 | 2021-01-05 | 东北农业大学 | Microbial preparation for promoting rooting and lodging resistance of rice and application thereof |
| CN112500220A (en) * | 2020-11-05 | 2021-03-16 | 东北农业大学 | Liquid compound microorganism foliar fertilizer for improving saline-alkali soil and application thereof |
| CN112500216A (en) * | 2020-11-02 | 2021-03-16 | 东北农业大学 | Bio-organic fertilizer with crop rooting promoting function and preparation method thereof |
| CN112573970A (en) * | 2020-11-05 | 2021-03-30 | 东北农业大学 | Solid-state compound microbial foliar fertilizer for improving saline-alkali soil and application thereof |
| CN112592222A (en) * | 2020-11-05 | 2021-04-02 | 东北农业大学 | Solid-state composite microorganism rooting agent for improving saline-alkali soil and application thereof |
| CN112794750A (en) * | 2021-01-11 | 2021-05-14 | 东北农业大学 | Foliar fertilizer for increasing potato yield and preparation method thereof |
| CN112830854A (en) * | 2021-02-22 | 2021-05-25 | 东北农业大学 | Foliar fertilizer for preventing and controlling fusarium solani and application thereof |
| CN113796391A (en) * | 2021-08-25 | 2021-12-17 | 北京东方力原生物科技有限公司 | Biological dormancy breaking agent |
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| CN113796391A (en) * | 2021-08-25 | 2021-12-17 | 北京东方力原生物科技有限公司 | Biological dormancy breaking agent |
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Application publication date: 20190521 |
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