CN116686903B - Complex bacterial enzyme biological starter and application thereof - Google Patents
Complex bacterial enzyme biological starter and application thereof Download PDFInfo
- Publication number
- CN116686903B CN116686903B CN202310966499.0A CN202310966499A CN116686903B CN 116686903 B CN116686903 B CN 116686903B CN 202310966499 A CN202310966499 A CN 202310966499A CN 116686903 B CN116686903 B CN 116686903B
- Authority
- CN
- China
- Prior art keywords
- starter
- parts
- complex
- fermentation
- bacterial liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 60
- 239000007858 starting material Substances 0.000 title claims abstract description 35
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 26
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 239000002131 composite material Substances 0.000 claims abstract description 43
- 238000000855 fermentation Methods 0.000 claims abstract description 36
- 241000894006 Bacteria Species 0.000 claims abstract description 33
- 239000000284 extract Substances 0.000 claims abstract description 24
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 23
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 23
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 23
- 230000004151 fermentation Effects 0.000 claims abstract description 22
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims abstract description 20
- 238000002156 mixing Methods 0.000 claims abstract description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000000463 material Substances 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 13
- 238000010564 aerobic fermentation Methods 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 238000009423 ventilation Methods 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000010992 reflux Methods 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 235000013379 molasses Nutrition 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims description 3
- 108010059820 Polygalacturonase Proteins 0.000 claims description 2
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- 240000004658 Medicago sativa Species 0.000 claims 2
- 241000219823 Medicago Species 0.000 abstract description 18
- 241000282887 Suidae Species 0.000 abstract description 17
- 150000001875 compounds Chemical class 0.000 abstract description 12
- 235000015277 pork Nutrition 0.000 abstract description 4
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 229940088598 enzyme Drugs 0.000 description 18
- 230000000052 comparative effect Effects 0.000 description 11
- 239000003242 anti bacterial agent Substances 0.000 description 7
- 229940088710 antibiotic agent Drugs 0.000 description 7
- 238000013329 compounding Methods 0.000 description 5
- 235000013372 meat Nutrition 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000419 plant extract Substances 0.000 description 4
- 238000007918 intramuscular administration Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003674 animal food additive Substances 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000010855 food raising agent Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001007 puffing effect Effects 0.000 description 2
- 108010077805 Bacterial Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000004957 immunoregulator effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000001937 non-anti-biotic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000015193 tomato juice Nutrition 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Inorganic Chemistry (AREA)
- Birds (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The application discloses a compound bacteria enzyme biological starter and application thereof, belonging to the field of microbial fermentation. The complex bacterial enzyme biological starter is prepared from complex bacterial liquid, alfalfa extract and pectase according to the weight ratio of 1: (0.5-2): mixing the components (1-3); the composite bacterial liquid is prepared from bacillus subtilis and streptococcus thermophilus according to the weight ratio of 1:1, and is compounded according to the proportion. According to the application, the composite bacteria combination commonly used in the fermented feed is compounded with pectase and alfalfa extract to obtain the starter, and the prepared composite bacteria antibiotic-free fermented feed can remarkably improve the pork quality of growing-finishing pigs after being fed by the synergistic fermentation of the pectase, the alfalfa extract and the alfalfa extract.
Description
Technical Field
The application belongs to the field of microbial fermentation, and particularly relates to a compound bacterium enzyme biological starter and application thereof.
Background
Antibiotic-free cultivation is short for antibiotic-free cultivation, and refers to the fact that any kind of antibiotics are not used in the cultivation process, in particular to the fact that human antibiotics and livestock antibiotics are not used. The antibiotic-free fermented feed is a biological fermented feed which integrates microorganism and compound enzyme microbial starter, and converts feed raw materials into microorganism bacterial protein, bioactive small peptide amino acid, microorganism active probiotics and compound enzyme preparation. The product can not only make up for amino acid which is easy to lack in the conventional feed, but also enable the nutritional ingredients of other coarse feed raw materials to be quickly converted, promote the reproduction of intestinal flora and inhibit the growth of pathogenic microorganisms, thereby replacing the use of antibiotics in daily ration and reducing the threat of the antibiotics to the environment and food safety. Antibiotic-free feeding of live pigs is a major trend and is a necessary requirement of related policy and regulations. At present, the adopted antibiotic-free feed mainly comprises the following modes: the treatment of the raw materials mainly comprises soybean puffing, corn puffing and the like.
The addition of enzyme preparations is also one of the methods for preparing antibiotic-free feed. The enzyme preparation is a biological product with catalytic function after purification and processing, is mainly used for catalyzing various chemical reactions in the production process, has the characteristics of high catalytic efficiency, high specificity, mild acting condition, energy consumption reduction, chemical pollution reduction and the like, is derived from organisms, is generally safer, and can be used in proper amount according to production needs. The enzyme preparation is added into the nonreactive fermented feed, and the digestion of the feed in the body is promoted by the enzyme preparation, so that the residence time in the gastrointestinal tract is shortened, and the opportunity of being utilized by the growth of harmful bacteria can be reduced.
The plant extract contains multiple active ingredients such as polyphenol, flavonoid, polysaccharide, etc., and has various biological properties such as antioxidant, antiinflammatory, and immunoregulatory. Recent studies have shown that plant extracts not only can enhance animal growth performance, but also play an important role in improving meat quality and flavor.
The application patent with publication number of CN114916613A discloses a composition of Chinese herbal medicine for replacing antibiotics and a preparation method of feed thereof; the application discloses a preparation process of a biological microbial inoculum capable of replacing feed antibiotics, which is disclosed in the publication No. CN 112725197B. Said application uses Chinese herbal medicine and living bacteria agent to prepare antibiotic-free feed. However, no report on the preparation of antibiotic-free feed by compounding a composite microbial inoculum, a plant extract and an enzyme preparation is currently seen.
Disclosure of Invention
Aiming at the defects of the prior art, the application aims to provide a complex bacterial enzyme biological starter and application thereof.
In order to achieve the above purpose, the application adopts the following technical scheme:
the application provides a complex bacterial enzyme biological starter, which is characterized by comprising complex bacterial liquid, alfalfa extract and pectinase according to the weight ratio of 1: (0.5-2): mixing the components (1-3); the composite bacterial liquid is prepared from bacillus subtilis and streptococcus thermophilus according to the weight ratio of 1:1, and is compounded according to the proportion.
Preferably, the viable count of Streptococcus thermophilus in the composite bacterial liquid is (1.5-2) multiplied by 10 10 CFU/g, the viable count of bacillus subtilis is (1-1.5). Times.10 10 CFU/g。
Preferably, the alfalfa extract is prepared by the following method:
drying and pulverizing herba Medicaginis, adding 10 times of ethanol solution into pulverized herba Medicaginis, reflux extracting for 2 hr, recovering ethanol from extractive solution, adding aluminum oxide to adsorb impurities, filtering, collecting filtrate, and spray drying the filtrate to obtain herba Medicaginis extract.
In a second aspect, the application provides an application of the complex bacterium enzyme biological starter in complex bacterium antibiotic-free fermented feed.
Preferably, the composite bacteria enzyme starter, brown sugar and water are added into the fermentation bed charge, and the mixture is uniformly mixed, and is subjected to aerobic fermentation for 6-8 hours and anaerobic fermentation for 10-14 days to prepare the composite bacteria antibiotic-free fermented feed.
Preferably, the fermentation base material consists of the following raw materials in parts by weight: 60 parts of corn flour, 24 parts of bean pulp, 10 parts of bran, 18 parts of corncob, 2 parts of molasses, 5 parts of rice bran, 1 part of ammonium sulfate and 5 parts of stone powder.
Preferably, the weight ratio of the complex bacterial enzyme starter, the brown sugar, the water and the fermentation base material is (0.05-0.2): (5-50): (10-50): (50-200).
Preferably, the aerobic fermentation conditions are as follows: the anaerobic fermentation conditions are that the temperature is 20-25 ℃, the ventilation rate is 2-3.5L/min: sealing and fermenting at 35-38deg.C.
The application has the beneficial effects that:
according to the application, the complex bacteria commonly used in the fermented feed are compounded with the pectase and the alfalfa extract to obtain the complex bacteria enzyme starter, on one hand, the pectase and the alfalfa extract not only can add nutrients to the feed, but also can be used as carriers of complex bacteria liquid, so that the complex bacteria is prevented from being inactivated; on the other hand, the composite bacteria antibiotic-free fermented feed prepared by fermenting the starter can obviously improve the pork quality of growing-finishing pigs after being fed.
Detailed Description
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
In the process of selecting raw materials, referring to a feed additive variety catalog (2013), the inventor firstly selects bacillus subtilis and streptococcus thermophilus which are commonly used for fermenting feed as compound bacterial liquid, then carries out a compounding experiment on the compound bacterial liquid, a plant extract and an enzyme preparation recorded in the feed additive variety catalog (2013), finally discovers that the compound bacterial liquid has a synergistic effect after being compounded with alfalfa extract and pectase, and the pork quality of growing-finishing pigs can be effectively improved by using the feed prepared by fermenting the three materials.
In order to enable those skilled in the art to more clearly understand the technical scheme of the present application, the technical scheme of the present application will be described in detail with reference to specific embodiments.
In the following examples and comparative examples, the bacillus subtilis used was purchased from China Center for Type Culture Collection (CCTCC) AB 130010, and after the bacillus subtilis was activated and cultured, a bacillus subtilis bacterial liquid was obtained; streptococcus thermophilus is purchased from China Center for Type Culture Collection (CCTCC) AB 2010206, and after the Streptococcus thermophilus is activated and cultured, streptococcus thermophilus bacterial liquid is obtained; pectase was purchased from Shanghai Seiyaka Biotechnology Co., ltd., specification BR,50000u/g.
The formula and the conditions of the culture medium of the bacillus subtilis are as follows: 100g of glucose, 20g of soybean peptone, 5g of NaCl, 1000mL of distilled water, pH of 7.0, culture time of 20h and culture temperature of 37 ℃; the culture medium formula and conditions of streptococcus thermophilus are as follows: 7.5g of yeast extract, 7.5g of peptone, 10g of glucose and KH 2 PO 4 2g, 100ml of tomato juice, 80ml of tween, 900ml of distilled water, pH of 7.0 and incubation time of 24h.
The fermentation base materials in the application examples and the comparative examples consist of the following raw materials in parts by weight: 60 parts of corn flour, 24 parts of bean pulp, 10 parts of bran, 18 parts of corncob, 2 parts of molasses, 5 parts of rice bran, 1 part of ammonium sulfate and 5 parts of stone powder.
Example 1: and (3) preparing the complex bacterial enzyme biological starter.
(1) Drying and pulverizing herba Medicaginis, adding 10 weight times of ethanol solution (volume fraction 60%) into pulverized herba Medicaginis, and reflux extracting for 2 hr; recovering ethanol from the extractive solution, adding aluminum oxide to adsorb impurities, filtering, collecting filtrate, and spray drying the filtrate to obtain herba Medicaginis extract.
(2) The bacillus subtilis bacterial liquid and the streptococcus thermophilus bacterial liquid are mixed according to the following proportionThe weight ratio of the light to the light is 1:1, and then mixing the composite bacterial liquid, the alfalfa extract and the pectase according to the weight ratio of 1:0.5:1, mixing the materials in proportion to obtain a starter; the viable count of the streptococcus thermophilus in the composite bacterial liquid is 1.5x10 10 CFU/g, viable count of bacillus subtilis is 1×10 10 CFU/g。
Example 2: and (3) preparing the complex bacterial enzyme biological starter.
(1) Drying and pulverizing herba Medicaginis, adding 10 weight times of ethanol solution (volume fraction 60%) into pulverized herba Medicaginis, and reflux extracting for 2 hr; recovering ethanol from the extractive solution, adding aluminum oxide to adsorb impurities, filtering, collecting filtrate, and spray drying the filtrate to obtain herba Medicaginis extract.
(2) The bacillus subtilis bacterial liquid and the streptococcus thermophilus bacterial liquid are mixed according to the weight ratio of 1:1, and then mixing the composite bacterial liquid, the alfalfa extract and the pectase according to the weight ratio of 1:1:2, mixing the materials in proportion to obtain a starter; the viable count of the streptococcus thermophilus in the composite bacterial liquid is 1.8x10 10 CFU/g, viable count of bacillus subtilis is 1.4X10 10 CFU/g。
Example 3: and (3) preparing the complex bacterial enzyme biological starter.
(1) Drying and pulverizing herba Medicaginis, adding 10 weight times of ethanol solution (volume fraction 60%) into pulverized herba Medicaginis, and reflux extracting for 2 hr; recovering ethanol from the extractive solution, adding aluminum oxide to adsorb impurities, filtering, collecting filtrate, and spray drying the filtrate to obtain herba Medicaginis extract.
(2) The bacillus subtilis bacterial liquid and the streptococcus thermophilus bacterial liquid are mixed according to the weight ratio of 1:1, and then mixing the composite bacterial liquid, the alfalfa extract and the pectase according to the weight ratio of 1:2:3, mixing the materials in proportion to obtain a starter; the viable count of the streptococcus thermophilus in the composite bacterial liquid is 2 multiplied by 10 10 CFU/g, viable count of bacillus subtilis is 1.5X10 10 CFU/g。
Application example 1: and (3) preparing the composite bacteria antibiotic-free fermented feed.
The weight ratio of the starter, the brown sugar, the water and the fermentation base material is 0.05:5:10:50, adding a starter, brown sugar and water into the fermentation bed charge, uniformly mixing, performing aerobic fermentation for 6 hours at 20 ℃ and ventilation of 2L/min, and performing anaerobic fermentation for 10 days at 35 ℃ to obtain the composite bacteria antibiotic-free fermented feed.
Application example 2: and (3) preparing the composite bacteria antibiotic-free fermented feed.
The weight ratio of the leavening agent to the brown sugar to the water to the fermentation bed charge is 0.1:24:26:100, adding a starter, brown sugar and water into the fermentation bed charge, uniformly mixing, performing aerobic fermentation for 7h at 22 ℃ and ventilation of 3L/min, and performing anaerobic fermentation for 12 days at 36 ℃ to obtain the composite bacteria antibiotic-free fermented feed.
Application example 3: and (3) preparing the composite bacteria antibiotic-free fermented feed.
The weight ratio of the leavening agent to the brown sugar to the water to the fermentation bed charge is 0.2:50:50:200, adding a starter, brown sugar and water into the fermentation base material, uniformly mixing, performing aerobic fermentation for 8 hours at 25 ℃ and ventilation of 3.5L/min, and performing anaerobic fermentation for 14 days at 38 ℃ to obtain the composite bacteria antibiotic-free fermented feed.
Comparative example 1:
the preparation of the composite bacterial anti-fermentation feed according to the method of application example 1 is characterized in that alfalfa extract and pectase are not added into the fermenting agent, and the specific steps are as follows:
(1) The bacillus subtilis bacterial liquid and the streptococcus thermophilus bacterial liquid are mixed according to the weight ratio of 1:1, mixing the materials in proportion to obtain a composite bacterial liquid; the viable count of the streptococcus thermophilus in the composite bacterial liquid is 1.5x10 10 CFU/g, viable count of bacillus subtilis is 1×10 10 CFU/g。
(2) According to the weight ratio of the composite bacterial liquid to the brown sugar to the water to the fermentation bed charge of 0.05:5:10:50, adding the composite bacterial liquid, brown sugar and water into the fermentation bed charge, uniformly mixing, performing aerobic fermentation for 6 hours at 20 ℃ and ventilation of 2L/min, and performing anaerobic fermentation for 10 days at 35 ℃ to obtain the composite bacterial antibiotic-free fermented feed.
Comparative example 2:
the preparation of the composite bacterial anti-fermentation feed according to the method of application example 1 is different in that alfalfa extract is not added into the starter, and the specific steps are as follows:
(1) The bacillus subtilis bacterial liquid and the streptococcus thermophilus bacterial liquid are mixed according to the weight ratio of 1:1, and then mixing the composite bacterial liquid and pectase according to the weight ratio of 1:1, mixing the materials in proportion to obtain a starter; the viable count of the streptococcus thermophilus in the composite bacterial liquid is 1.5x10 10 CFU/g, viable count of bacillus subtilis is 1×10 10 CFU/g。
(2) The weight ratio of the starter, the brown sugar, the water and the fermentation base material is 0.05:5:10:50, adding a starter, brown sugar and water into the fermentation bed charge, uniformly mixing, performing aerobic fermentation for 6 hours at 20 ℃ and ventilation of 2L/min, and performing anaerobic fermentation for 10 days at 35 ℃ to obtain the composite bacteria antibiotic-free fermented feed.
Comparative example 3:
the preparation of the compound bacteria anti-fermentation feed according to the method of application example 1 is different in that pectase is not added into the ferment, and the specific steps are as follows:
(1) The bacillus subtilis bacterial liquid and the streptococcus thermophilus bacterial liquid are mixed according to the weight ratio of 1:1, and then mixing the composite bacterial liquid and alfalfa extract according to the weight ratio of 1: mixing the materials according to a proportion of 0.5 to obtain a starter; the viable count of the streptococcus thermophilus in the composite bacterial liquid is 1.5x10 10 CFU/g, viable count of bacillus subtilis is 1×10 10 CFU/g。
(2) The weight ratio of the starter, the brown sugar, the water and the fermentation base material is 0.05:5:10:50, adding a starter, brown sugar and water into the fermentation bed charge, uniformly mixing, performing aerobic fermentation for 6 hours at 20 ℃ and ventilation of 2L/min, and performing anaerobic fermentation for 10 days at 35 ℃ to obtain the composite bacteria antibiotic-free fermented feed.
Test example: feeding test of growing and fattening pigs.
(1) The test method comprises the following steps:
and selecting 60 healthy growing-finishing pigs with the body condition close to that of the growing-finishing pigs, numbering from 1 to 60, randomly dividing the growing-finishing pigs into 6 groups of 10 growing-finishing pigs, feeding the growing-finishing pigs in separate houses, and feeding the growing-finishing pigs in the same group into the same pig house. The temperature of each pig house is controlled to be 25 ℃, the relative humidity is controlled to be 70%, other growing environment conditions of each group of pigs are guaranteed to be completely consistent, all growing and fattening pigs are fed three times a day in the morning, in the evening, the feeding amount is 2kg a day, water is freely drunk, the manure is cleaned twice a day, the house is kept clean, and the experiment is carried out for 30 days.
The feeds for groups 1-6 were as follows:
group 1: feeding the composite bacteria antibiotic-free fermented feed prepared in application example 1; group 2: feeding the composite bacteria antibiotic-free fermented feed prepared in application example 2; group 3: feeding the composite bacteria non-antibiotic fermented feed prepared in application example 3; group 4: feeding the composite bacteria antibiotic-free fermented feed prepared in the comparative example 1; group 5: feeding the composite bacteria antibiotic-free fermented feed prepared in the comparative example 2; group 6: the composite bacteria antibiotic-free fermented feed prepared in the comparative example 3 is fed.
Weighing and recording each growing-finishing pig after 30 days, calculating daily gain of each pig in each group of growing-finishing pigs, taking an average value of the results, and calculating feed-meat ratio; then, 2 pigs were slaughtered randomly for each group, and the eye muscle area, intramuscular fat and drip loss were measured, and the results were averaged.
Wherein, the eye muscle area and intramuscular fat are measured by the method described in the paper of alfalfa on the expression of genes related to pig physiology and meat quality, and the drip loss is measured by the method described in the paper of three additives on the production performance of fattening pigs and the influence of meat quality on intestinal microorganisms.
(2) Test results:
table: meat quality and feed ratio data of 1-6 groups
As shown in the table, compared with the 4-6 groups, the data of the eye muscle area and intramuscular fat in the data of the 1-3 groups are larger, the data of feed conversion ratio and drip loss are smaller, and the results show that the composite bacteria anti-fermentation feed prepared by feeding the application examples 1-3 can effectively improve the pork quality of growing and fattening pigs and has better feed effect.
Meanwhile, the feed of the 4 th group is prepared by fermenting the compound bacterial liquid in the comparative example 1, the feed of the 5 th group is prepared by fermenting the compound bacterial liquid in the comparative example 2 and pectase in a compounding way, the feed of the 6 th group is prepared by fermenting the compound bacterial liquid in the comparative example 3 and the alfalfa extract in a compounding way, and the 1 st group and the 4 th-6 th groups are compared with each other, so that the synergistic effect of improving the feed effect is achieved by compounding the compound bacterial liquid, the alfalfa extract and the pectase used in the application.
The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (5)
1. The complex bacterial enzyme biological starter is characterized by comprising complex bacterial liquid, alfalfa extract and pectinase according to the weight ratio of 1: (0.5-2): mixing the components (1-3); the composite bacterial liquid is prepared from bacillus subtilis and streptococcus thermophilus according to the weight ratio of 1:1, and is compounded according to the proportion;
the strain number of the bacillus subtilis is CCTCC AB 130010, and the strain number of the streptococcus thermophilus is CCTCC AB 2010206;
the alfalfa extract is prepared by the following method:
drying and pulverizing herba Medicaginis, adding 10 times of ethanol solution with volume fraction of 60% into pulverized herba Medicaginis, reflux extracting for 2 hr, recovering ethanol from the extractive solution, adding aluminum oxide to adsorb impurities, filtering, collecting filtrate, and spray drying the filtrate to obtain herba Medicaginis extract.
2. A complex bacterial enzyme according to claim 1The object ferment is characterized in that the viable count of streptococcus thermophilus in the composite bacterial liquid is (1.5-2) multiplied by 10 10 CFU/g, the viable count of bacillus subtilis is (1-1.5). Times.10 10 CFU/g。
3. Use of a complex bacterial enzyme biological starter according to any one of claims 1-2 for the preparation of a complex bacterial antibiotic-free fermented feed.
4. The use according to claim 3, wherein the complex bacteria enzyme starter, brown sugar and water are added into the fermentation base material, and the mixture is uniformly mixed, and the mixture is subjected to aerobic fermentation for 6-8 hours and anaerobic fermentation for 10-14 days to prepare the complex bacteria antibiotic-free fermented feed;
the fermentation bed charge consists of the following raw materials in parts by weight: 60 parts of corn flour, 24 parts of bean pulp, 10 parts of bran, 18 parts of corncob, 2 parts of molasses, 5 parts of rice bran, 1 part of ammonium sulfate and 5 parts of stone powder;
the weight ratio of the complex bacterial enzyme starter, the brown sugar, the water and the fermentation bed charge is (0.05-0.2): (5-50): (10-50): (50-200).
5. The use according to claim 4, wherein the aerobic fermentation conditions are: the anaerobic fermentation conditions are that the temperature is 20-25 ℃, the ventilation rate is 2-3.5L/min: sealing and fermenting at 35-38deg.C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310966499.0A CN116686903B (en) | 2023-08-03 | 2023-08-03 | Complex bacterial enzyme biological starter and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310966499.0A CN116686903B (en) | 2023-08-03 | 2023-08-03 | Complex bacterial enzyme biological starter and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116686903A CN116686903A (en) | 2023-09-05 |
| CN116686903B true CN116686903B (en) | 2023-10-10 |
Family
ID=87831464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310966499.0A Active CN116686903B (en) | 2023-08-03 | 2023-08-03 | Complex bacterial enzyme biological starter and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116686903B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150080983A (en) * | 2014-01-02 | 2015-07-13 | 고려대학교 산학협력단 | Fermented Castanea crenata inner shell extracts for anti-obesity and manufacturing method thereof |
| CN104938784A (en) * | 2015-06-26 | 2015-09-30 | 绥化学院 | Method for preparing animal probiotics fodder by fermenting edible mushroom waste, straw and pasture |
| CN110432294A (en) * | 2019-08-26 | 2019-11-12 | 厦门市民安创享实业有限公司 | A kind of bacteriostatic agent of biofermentation, preparation method and application |
| WO2020058223A1 (en) * | 2018-09-17 | 2020-03-26 | Dsm Ip Assets B.V. | Animal feed composition and use thereof |
| CN112136974A (en) * | 2020-09-28 | 2020-12-29 | 山东胜伟盐碱地科技有限公司 | Livestock feed and preparation method thereof |
| CN114052116A (en) * | 2022-01-18 | 2022-02-18 | 山东健源生物科技有限公司 | Probiotic plant extract compound biological agent and preparation method and application thereof |
| WO2022081947A1 (en) * | 2020-10-16 | 2022-04-21 | Dupont Nutrition Biosciences | Feed compositions for animal health |
-
2023
- 2023-08-03 CN CN202310966499.0A patent/CN116686903B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150080983A (en) * | 2014-01-02 | 2015-07-13 | 고려대학교 산학협력단 | Fermented Castanea crenata inner shell extracts for anti-obesity and manufacturing method thereof |
| CN104938784A (en) * | 2015-06-26 | 2015-09-30 | 绥化学院 | Method for preparing animal probiotics fodder by fermenting edible mushroom waste, straw and pasture |
| WO2020058223A1 (en) * | 2018-09-17 | 2020-03-26 | Dsm Ip Assets B.V. | Animal feed composition and use thereof |
| CN110432294A (en) * | 2019-08-26 | 2019-11-12 | 厦门市民安创享实业有限公司 | A kind of bacteriostatic agent of biofermentation, preparation method and application |
| CN112136974A (en) * | 2020-09-28 | 2020-12-29 | 山东胜伟盐碱地科技有限公司 | Livestock feed and preparation method thereof |
| WO2022081947A1 (en) * | 2020-10-16 | 2022-04-21 | Dupont Nutrition Biosciences | Feed compositions for animal health |
| CN114052116A (en) * | 2022-01-18 | 2022-02-18 | 山东健源生物科技有限公司 | Probiotic plant extract compound biological agent and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116686903A (en) | 2023-09-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101711548B (en) | Fermented feed containing sodium humate and process and application thereof | |
| CN106974063B (en) | Method for producing high-efficiency protein feed by using feed enzyme in cooperation with bacillus coagulans | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| CN111436526A (en) | Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs | |
| CN102805209A (en) | Synbiotics feed additive for beasts and birds and application thereof | |
| CN112136965B (en) | Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof | |
| CN101843297A (en) | Biological fermentation process for improving protein content of cottonseed meal and detoxicating cottonseed meal | |
| CN110583866A (en) | High-dietary-fiber low-antigen-protein fermented soybean hull and preparation method thereof | |
| CN115067419A (en) | Application of feed additive and preparation method of earthworm fermentation liquor | |
| CN115074290B (en) | Lactobacillus casei for co-production of phenyllactic acid and gamma-aminobutyric acid and application thereof | |
| CN111134231B (en) | Bacillus Zhangzhou and method for fermenting mulberry leaf powder by using same | |
| CN101194668B (en) | Process for preparing blood meal biological modified peptide protein and application of the same | |
| CN112961806B (en) | Bacillus coagulans for high-yield lactic acid, biological fermentation feed and preparation method and application thereof | |
| CN111296723A (en) | Probiotic agent for multi-strain combined solid state fermentation of rapeseed meal and fermentation method thereof | |
| CN114601041A (en) | Highland barley vinasse fermented feed for improving yak meat quality and preparation method and application thereof | |
| CN113508872B (en) | Palm meal raw material biological pretreatment method | |
| CN113773996A (en) | Preparation method and application of feeding bacillus subtilis | |
| CN112790274A (en) | Fermented paper mulberry feed and preparation method and application thereof | |
| KR20000073915A (en) | Probiotics For Feed Additives | |
| CN116391796A (en) | Method for processing wangcao | |
| CN110870511A (en) | Dry-wet mixed concentrated feed and preparation method thereof | |
| CN116686903B (en) | Complex bacterial enzyme biological starter and application thereof | |
| CN110859240A (en) | Dry-wet mixed complete feed and preparation method thereof | |
| KR101252134B1 (en) | Feed additives for promoting growth of cattle and process for the preparation of feed for breeding cattle using the same | |
| CN103710413A (en) | Preparation method of lactobacillus peptide as well as application of lactobacillus peptide in animal feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |