CN101843297A - Biological fermentation process for improving protein content of cottonseed meal and detoxicating cottonseed meal - Google Patents
Biological fermentation process for improving protein content of cottonseed meal and detoxicating cottonseed meal Download PDFInfo
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- CN101843297A CN101843297A CN201010185286A CN201010185286A CN101843297A CN 101843297 A CN101843297 A CN 101843297A CN 201010185286 A CN201010185286 A CN 201010185286A CN 201010185286 A CN201010185286 A CN 201010185286A CN 101843297 A CN101843297 A CN 101843297A
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- cotton dregs
- cottonseed meal
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention relates to a biological fermentation process for improving the protein content of cottonseed meal and detoxicating the cottonseed meal, which is characterized in that: the byproduct of cottonseed subjected to oil extraction, namely the cottonseed meal is taken as a raw material; the cottonseed meal is processed by adopting microbial fermentation technology; a cottonseed meal mixed fermentation process is adopted; strains are combined, cell walls of a plant are broken and free gossypol and anti-nutritional factors contained in the cottonseed meal, such as cyclopropene fatty acid, phytic acid, tannin, non-starch polysaccharide and non-glycan are modified, transferred, recombinated, purified and detoxicated; and proteins in the cottonseed meal which have large molecular weight and are difficult to absorb by animals are degraded into small active peptides by a biological enzymolysis method, and probiotics, digestive enzymes, lactic acid and various bioactive factors in the product are kept. The biological fermentation process can greatly improve the protein content of the cottonseed meal and effectively achieve the detoxicating effect.
Description
Technical field
The present invention relates to a kind of biological fermentation process, but particularly a kind of also biological fermentation process of detoxification that improves the cotton dregs protein content.
Background technology
The existing continuous dregs of rice extract albumen and generally adopt " soaking " method to extract, and method is cottonseed-pulverizings-molten soaking-separate-filter-steam accent pH value-precipitation-separation-drying-go out cottonseed protein.The shortcoming of this method is: do not add the water cottonseed protein during pulverizing because of the high humidity sex change, influenced the extraction of protein; Steam has directly reduced the quality and the recovery rate of protein by the high temperature of filtrate porch; In addition, the method can't be removed the gossypol in the cottonseed.Because gossypol is poisonous, cause extraction rate of protein low, therefore, the detoxification of cottonseed also is a key issue.At present, ferrous sulfate detoxicity method, oxidant detoxicity method etc. are arranged, have shortcomings such as equipment investment is excessive, forfeiture nutritional labeling.
Summary of the invention
In order to solve the problem that prior art cotton dregs protein extracting ratio is low and detoxification runs into, improve its nutritive value and applicability, realize the comprehensive utilization of cotton dregs discarded object, but a kind of biological fermentation process that improves cotton dregs protein content and detoxification is proposed in the present invention.
For addressing the above problem, the technical scheme that the present invention takes is: but a kind of biological fermentation process that improves cotton dregs protein content and detoxification, it is characterized in that with the byproduct cotton dregs after the cottonseed oil expression be raw material, adopt microbial fermentation technology that cotton dregs are handled, abolish plant cell wall, to cotton dregs contained free gossypol and ANFs: encircle third rare aliphatic acid, phytic acid, tannin, SNSP and non-glycan are modified, shift, reorganization, the purifying detoxification treatment, by the difficult macromolecular weight protein that is absorbed by animal in the biologic enzymolysis method degraded cotton dregs is active small peptide, probio in the retained product, digestive ferment, lactic acid and multiple bioactie agent, its technological process is: pulverizing feeds intake → promote mixes → enters feed bin → pulverizing processing → segmentation stirring → fermentation and feed intake → enter feed bin → small powder mixing → conveying than cage → humidification stirring → product fermentation → pneumatic conveying drying → cyclonic separation → fine powder discharging → dry materials → quality chemical examination → packing of product;
Selected cotton dregs free gossypol degradation bacteria strains B2 is Bacillus cereus vinegar shape bacillus, fermentation period 48h, and material-water ratio 1: 1, inoculum concentration 2%, 28.0 ℃ of temperature, initial ph value is a nature, and the free gossypol degradation rate is reached maximum, is 51.5%;
Selected cotton dregs protein degradation bacterial strain B1 is Bacillus subtilis bacillus subtilis; Adopt the fermentation of single bacterium, fermentation period 72h, material-water ratio 1: 1, inoculum concentration 4%, room temperature 〉=25 ℃, initial ph value is a nature, the TCA-NSI index reaches 33.0%;
Selected saccharomycete Y2 and lactic acid bacteria L;
Adopt the cotton dregs mixed fungus fermentation, the combination bacterial classification: bacillus subtilis B1+ vinegar shape bacillus B2+ saccharomycete Y2+ lactic acid bacteria L, four kinds of bacterium are all by 1% inoculation of wet feed, and fermentation period is 48h, material-water ratio 1: 1.2,30.0 ℃ of temperature, initial ph value is a nature.
Cotton dregs are pulverized and are selected sieve aperture Φ 0.8～1.0mm sieve sheet for use;
Thin up, material-water ratio 1: 1.2; Water temperature is controlled at 35～40 ℃; Water quality pH value 6.5～7.0.
Oven dry is handled, oven dry water content 8～10%, crude protein 〉=60%, lactic acid bacteria number 〉=4 * 107cfu/g.
Index detects, the little acid of smell, and moisture content is 41～45%; PH value is between 4.5～5.0; Lactic acid bacteria number 〉=4.5 * 109cfu/g.
The invention has the beneficial effects as follows: in the fermentation process cotton dregs raw material has been carried out enzymolysis processing, effectively improved crude protein content, can substitute tradition high price forage protein raw materials such as fish meal, dregs of beans fully; Improve its palatability, eliminating influences the mouthfeel bitter taste in a large number; The a large amount of active probiotics that produce in the detoxification sweat can be regulated the body gastrointestinal tract microecological balance and improve digestive enzyme activity, improve the feed protein quality; Alternative antibiotics production nonreactive biological fermentation feed; Improve immunity of livestock, can effectively reduce M ﹠ M; Can reduce the excretion of nitrogen in the ight soil, phosphorus, calcium, reduce ight soil stink and noxious gas emission, thereby reduce environmental pollution; Improve the meat, eggs and milk quality; Because the cotton dregs relative low price with high price animal or plant protein raw materials such as fermentation cotton dregs raw material substitution fish meal and dregs of beans, can significantly reduce the production cost of feed formula.By the complete production unit of special use, solve that the traditional zymotic technology intermission is long, power consumption is big, cost is high, when taking a lot of work, problem such as yield poorly, satisfy the needs that industrialization is produced, reduce the traditional zymotic technical fee more than 30%.
The present invention will be further described below in conjunction with process chart and embodiment.
Description of drawings
Accompanying drawing 1 is the process chart that the process of high protein cotton dregs feed is produced in invention.
The specific embodiment
The present invention adopts microbial fermentation technology that cotton dregs are handled, abolish plant cell wall, to cotton dregs contained free gossypol and other ANFs, as encircle detoxification treatment such as third rare aliphatic acid (CPFA), phytic acid, tannin, SNSP and non-glycan etc. are modified, shifted, reorganization, purifying, to improve the digestive utilization ratio and the material protein quality of vegetable protein; By the difficult macromolecular weight protein that is absorbed by animal in the biologic enzymolysis method degraded cotton dregs is active small peptide, probio, digestive ferment, lactic acid and multiple bioactie agent in the retained product.
The present invention adopts the method for microbial fermentation, the toxicants such as free gossypol in the cotton dregs are removed in degraded, simultaneously the macromolecular weight protein in the cotton dregs is decomposed into the small molecular weight protein or the peptide of easier absorption, and obtain the metabolite useful, thereby improve the nutritive value and the adding proportion in feed is cultured of cotton dregs to growth of animal.
With reference to accompanying drawing 1, but the biological fermentation process technological process that improves cotton dregs protein content and detoxification is: pulverizing feeds intake → promote mixes → enters feed bin → pulverizing processing → segmentation stirring → fermentation and feed intake → enter feed bin → small powder mixing → conveying than cage → humidification stirring → product fermentation → pneumatic conveying drying → cyclonic separation → fine powder discharging → dry materials → chemical examination → pack; In specific culture medium and environment,, select, integrate, cultivate, expand numerous to microbial population by microbial nutrition prescription and process conditions control.
Cross automatic control, finish the technical process of breeding → inoculation → fermentation → detoxification → oven dry → finished product temperature, humidity, oxygen and time.
By abolishing the cotton dregs plant cell wall, cotton dregs free gossypol and other ANFs are removed in degraded, improve the digestive utilization ratio of vegetable protein, increase substantially the material protein quality.
By biologic enzymolysis method high molecular weight protein is degraded into micromolecular oligopeptides, improves value of the product.
Cotton dregs quality: protein content 〉=60%.
Cotton dregs are pulverized: sieve sheet sieve aperture Φ 0.8～1.0mm.
Thin up: material-water ratio 1: 1.2; Water temperature was controlled at about 35～37 ℃ (37～40 ℃ of winters); Water quality pH value 6.5～7.0.
Bacterial classification preparation: cotton dregs free gossypol degradation bacteria strains B2, selected vinegar shape bacillus (Bacilluscereus); Cotton dregs protein degradation bacterial strain B1, selected bacillus subtilis (Bacil lus subtilis); Simultaneously, selected can produce good ferment local-flavor road bacterial strain and be respectively saccharomycete Y2 and lactic acid bacteria L, adopt the cotton dregs mixed fungus fermentation, four kinds of bacterium of combination bacterial classification all inoculate by 1% of wet feed.
Strain fermentation: room temperature 〉=25 ℃, to ferment 48 hours, initial ph value is a nature.
Index detects: the little acid of smell; Moisture content is 41～45%; PH value is between 4.5～5.0; Lactic acid bacteria number 〉=4.5 * 109cfu/g.
Oven dry is handled: oven dry water content 8～10%, crude protein 〉=60%, lactic acid bacteria number 〉=4 * 107cfu/g.
Cotton dregs free gossypol degradation bacteria strains B2, Bacillus cereus vinegar shape bacillus, its fermentation period 48h, material-water ratio 1: 1, inoculum concentration 2%, 28.0 ℃ of temperature, initial ph value is a nature, and the free gossypol degradation rate is reached maximum, is 51.5%;
Cotton dregs protein degradation bacterial strain B1, Bacillus subtilis bacillus subtilis; Select single bacterium zymotechnique for use, fermentation period 72h, material-water ratio 1: 1, inoculum concentration 4%, 30.0 ℃ of temperature, initial ph value is a nature, best to cotton dregs protein degradation effect, the TCA-NSI index reaches 33.0%;
Cotton dregs mix the combination bacterial classification of bacterium, both bacillus subtilis B1+ vinegar shape bacillus B2+ saccharomycete Y2+ lactic acid bacteria L, and four kinds of bacterium all inoculate by 1% of wet feed, and fermentation period is 48h, material-water ratio 1: 1.2,30.0 ℃ of temperature, initial ph value is a nature.
With byproduct one cotton dregs after the cottonseed oil expression is raw material, adopt microbial fermentation technology that cotton dregs are handled, abolish plant cell wall, to cotton dregs contained free gossypol and ANFs: encircle that third rare aliphatic acid, phytic acid, tannin, SNSP and non-glycan are modified, shifted, reorganization, purifying detoxification treatment, by the difficult macromolecular weight protein that is absorbed by animal in the biologic enzymolysis method degraded cotton dregs is active small peptide, probio, digestive ferment, lactic acid and multiple bioactie agent in the retained product.
Adopt biological fermentation process of the present invention, its product can illustrate with following data:
Table 1: the nutrition contrast before and after the cotton dregs fermentation
| Raw material | The dregs of beans nutritional labeling | Cotton dregs before the fermentation | Fermentation back cotton dregs |
| Crude protein % | ??43.00 | ??40.20 | ??60.0 |
| Lysine % | ??2.45 | ??1.58 | ??1.75 |
| Methionine % | ??0.64 | ??0.45 | ??0.63 |
| Crude fibre % | ??4.5％ | ??12.52 | ??9.02 |
| NFE % | ??29 | ??29.9 | ??23.4 |
| Thiamine | ??2.0 | ??7.78 | ??12.45 |
| Raw material | The dregs of beans nutritional labeling | Cotton dregs before the fermentation | Fermentation back cotton dregs |
| Riboflavin | ??3.2 | ??4.2 | ??14.5 |
| Nicotinic acid | ??32 | ??38 | ??121.2 |
| Pantothenic acid | ??15 | ??7.8 | ??22.4 |
| Choline | ??2796 | ??2754 | ??2854 |
Table 2: fermentation cotton dregs main component index
| Project | Crude protein | Crude fibre | Calcium | Total phosphorus | Lysine | Methionine | Arginine | Free gossypol |
| ??％ | ??60.0 | ??9.0 | ??0.28 | ??1.04 | ??2.15 | ??0.65 | ??4.30 | ??0.02 |
Table 3: the nutrition contrast before and after the cotton dregs fermentation
| Project | Cotton dregs before the fermentation | Fermentation back cotton dregs |
| Free gossypol | ??852mg/kg | ??203mg/kg |
| Glucosinolate | ??2.10％ | ??0.51％ |
| Isothiocyanates | ??0.36％ | ??0.03％ |
| Oxazolidine thione | ??0.24％ | ??0.05％ |
| Phytic acid | ??4.98％ | ??0.97％ |
| Tannin | ??1.56％ | ??0.34％ |
| Sinapine | ??1.20％ | ??0.54％ |
| Saponin | ??0.62％ | ??0.35％ |
Claims (5)
1. but one kind is improved the also biological fermentation process of detoxification of cotton dregs protein content, it is characterized in that: with the byproduct cotton dregs after the cottonseed oil expression is raw material, adopt microbial fermentation technology that cotton dregs are handled, abolish plant cell wall, to cotton dregs contained free gossypol and ANFs: encircle third rare aliphatic acid, phytic acid, tannin, SNSP and non-glycan are modified, shift, reorganization, the purifying detoxification treatment, by the difficult macromolecular weight protein that is absorbed by animal in the biologic enzymolysis method degraded cotton dregs is active small peptide, probio in the retained product, digestive ferment, lactic acid and multiple bioactie agent, its technological process is: pulverizing feeds intake → promote mixes → enters feed bin → pulverizing processing → segmentation stirring → fermentation and feed intake → enter feed bin → small powder mixing → conveying than cage → humidification stirring → product fermentation → pneumatic conveying drying → cyclonic separation → fine powder discharging → dry materials → quality chemical examination → packing of product;
Selected cotton dregs free gossypol degradation bacteria strains B2 is Bacillus cereus vinegar shape bacillus, fermentation period 48h, and material-water ratio 1: 1, inoculum concentration 2%, 28.0 ℃ of temperature, initial ph value is a nature, and the free gossypol degradation rate is reached maximum, is 51.5%;
Selected cotton dregs protein degradation bacterial strain B1 is Bacillus subtilis bacillus subtilis; Adopt the fermentation of single bacterium, fermentation period 72h, material-water ratio 1: 1, inoculum concentration 4%, room temperature 〉=25 ℃, initial ph value is a nature, the TCA-NSI index reaches 33.0%;
Selected saccharomycete Y2 and lactic acid bacteria L;
Adopt the cotton dregs mixed fungus fermentation, the combination bacterial classification: bacillus subtilis B1+ vinegar shape bacillus B2+ saccharomycete Y2+ lactic acid bacteria L, four kinds of bacterium are all by 1% inoculation of wet feed, and fermentation period is 48h, material-water ratio 1: 1.2,30.0 ℃ of temperature, initial ph value is a nature.
2. but a kind of biological fermentation process that improves cotton dregs protein content and detoxification according to claim 1 is characterized in that: cotton dregs are pulverized and are selected sieve aperture Φ 0.8～1.0mm sieve sheet for use.
3. but a kind of biological fermentation process that improves cotton dregs protein content and detoxification according to claim 1 is characterized in that: thin up, material-water ratio 1: 1.2; Water temperature is controlled at 35～40 ℃; Water quality pH value 6.5～7.0.
4. but a kind of biological fermentation process that improves cotton dregs protein content and detoxification according to claim 1, it is characterized in that: oven dry is handled, oven dry water content 8～10%, crude protein 〉=60%, lactic acid bacteria number 〉=4 * 107cfu/g.
But 5. a kind of biological fermentation process that improves cotton dregs protein content and detoxification according to claim 1, it is characterized in that: index detects, the little acid of smell, moisture content is 41～45%; PH value is between 4.5～5.0; Lactic acid bacteria number 〉=4.5 * 109cfu/g.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318737A (en) * | 2011-10-11 | 2012-01-18 | 广东希普生物科技股份有限公司 | Preparation method of nontoxic cotton dreg animal feed |
| CN102805208A (en) * | 2012-09-06 | 2012-12-05 | 新疆希普生物科技股份有限公司 | Preparation method of high-lysine fermentation and detoxification cottonseed meal |
| CN103340288A (en) * | 2013-07-12 | 2013-10-09 | 安徽大学 | Method for degrading gossypol in cottonseed cake meal by using oxidase |
| CN103907750A (en) * | 2014-04-21 | 2014-07-09 | 新疆库尔勒正先农业投资有限公司 | Preparation method of microbial fermentation cotton seed meal |
| CN106071106A (en) * | 2016-06-17 | 2016-11-09 | 四川伯禹生物工程有限公司 | Fermentation cottonseed protein and the method for solid-state aerobic fermentation preparation fermentation cottonseed protein |
| CN109112077A (en) * | 2017-06-26 | 2019-01-01 | 中南林业科技大学 | One bacillus subtilis strain and its application in degradation tung oil tree cake protein |
| CN110250322A (en) * | 2019-07-18 | 2019-09-20 | 安徽笑果农牧产业科技有限公司 | A kind of method that industry bionic rumen is used for miscellaneous dregs of rice detoxification fermentation |
| CN112219934A (en) * | 2020-09-25 | 2021-01-15 | 新疆希普生物科技股份有限公司 | Method for producing cottonseed protein by enzymolysis and fermentation |
| CN113841785A (en) * | 2021-08-18 | 2021-12-28 | 福建傲农生物科技集团股份有限公司 | Preparation method and application of probiotic fermented cottonseed meal |
-
2010
- 2010-05-28 CN CN201010185286A patent/CN101843297A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318737A (en) * | 2011-10-11 | 2012-01-18 | 广东希普生物科技股份有限公司 | Preparation method of nontoxic cotton dreg animal feed |
| CN102318737B (en) * | 2011-10-11 | 2013-01-23 | 广东希普生物科技股份有限公司 | Preparation method of nontoxic cottonseed meal animal feed |
| CN102805208A (en) * | 2012-09-06 | 2012-12-05 | 新疆希普生物科技股份有限公司 | Preparation method of high-lysine fermentation and detoxification cottonseed meal |
| CN103340288A (en) * | 2013-07-12 | 2013-10-09 | 安徽大学 | Method for degrading gossypol in cottonseed cake meal by using oxidase |
| CN103340288B (en) * | 2013-07-12 | 2014-08-27 | 安徽大学 | Method for degrading gossypol in cottonseed cake meal by using oxidase |
| CN103907750A (en) * | 2014-04-21 | 2014-07-09 | 新疆库尔勒正先农业投资有限公司 | Preparation method of microbial fermentation cotton seed meal |
| CN106071106A (en) * | 2016-06-17 | 2016-11-09 | 四川伯禹生物工程有限公司 | Fermentation cottonseed protein and the method for solid-state aerobic fermentation preparation fermentation cottonseed protein |
| CN106071106B (en) * | 2016-06-17 | 2019-12-13 | 四川伯禹生物工程有限公司 | Fermented cottonseed protein and method for preparing fermented cottonseed protein by solid-state aerobic fermentation |
| CN109112077A (en) * | 2017-06-26 | 2019-01-01 | 中南林业科技大学 | One bacillus subtilis strain and its application in degradation tung oil tree cake protein |
| CN109112077B (en) * | 2017-06-26 | 2021-06-25 | 中南林业科技大学 | Bacillus subtilis strain and application thereof in degradation of tung oil cake protein |
| CN110250322A (en) * | 2019-07-18 | 2019-09-20 | 安徽笑果农牧产业科技有限公司 | A kind of method that industry bionic rumen is used for miscellaneous dregs of rice detoxification fermentation |
| CN112219934A (en) * | 2020-09-25 | 2021-01-15 | 新疆希普生物科技股份有限公司 | Method for producing cottonseed protein by enzymolysis and fermentation |
| CN112219934B (en) * | 2020-09-25 | 2021-07-16 | 新疆希普生物科技股份有限公司 | Method for producing cottonseed protein by enzymolysis and fermentation |
| CN113841785A (en) * | 2021-08-18 | 2021-12-28 | 福建傲农生物科技集团股份有限公司 | Preparation method and application of probiotic fermented cottonseed meal |
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Application publication date: 20100929 |