CN106260540A - A kind of biological feedstuff for creep feed and creep feed - Google Patents
A kind of biological feedstuff for creep feed and creep feed Download PDFInfo
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Abstract
The invention discloses a kind of biological feedstuff for creep feed, by the feedstuff of water content 20 50% at a temperature of 15 DEG C 35 DEG C, sealing and fermenting 3 10d prepares, wherein, in tunning, containing viable count >=1 × 109Individual/g, containing organic acid >=2%, described feedstuff contains the component of following weight proportioning: yeast 0.05 0.2g/kg, lactic acid bacteria 0.05 0.2g/kg, bacillus cereus 0.05 0.2g/kg, expanded dehulled soybean meal 500 800g/kg, Semen Maydis powder 50 100g/kg, Testa Tritici 100 300g/kg.The present invention 5% 10% produces for creep feed complete feedstuff, alternative fish flour, and the creep feed prepared is balanced in nutrition, digestibility is high, decrease the application of antibiotic, diarrhea rate and the mortality rate of suckling piglet can be effectively reduced, the daily gain of suckling piglet and the improvement of environment are had positive meaning.
Description
Technical field
The invention belongs to field of animal feed, particularly to a kind of biological feedstuff for creep feed
And it is added with the creep feed of this biological feedstuff.
Background technology
Biological feedstuff i.e. fermented feed is the heat of herding worker's research and development the most both at home and abroad
Point, the most rationally application China feedstuff resource, reinforcement feedstuff and livestock product safety are raw
Produce, promote that all many-sides such as plant's reduction of discharging, reduction environmental pollution all show well
Prospect, therefore development biological feedstuff has great strategy meaning for livestock both domestic and external
Justice.It is reported that external biological feedstuff research just has begun to starting from the eighties in 20th century, but
Be that true development gets up but it is after the nineties in 20th century.China grinds for biological feedstuff
Study carefully and start late, start from the time in recent years.The activated feed additive research of foreign biomolecule
Development progress is rapid, and presently the aggregate level in industry is higher than domestic, especially feeding
The level of relative of the physiological and biochemical research aspect foreign study person of bioactive additive is higher.
And the research of China's biological feedstuff is in addition to minority traditional zymotic product, generally there is production technology
The problems such as level is the most perfect, kind is few, application technology does not matches.However as row
The development of industry and progress, the exploitation either theoretical research of current China biological feedstuff or reality
Border application all presents the situation of high speed development.A large amount of along with modern biotechnology extensively should
With, in the fermentation strain screen of enzyme preparation, the improvement of fermentation technology and the progress of the level of production
Etc. aspect caught up with even certain fields and alreadyd more than the respective horizontal of developed country, as resistance to
Macrotherm phytase naked enzyme production technology etc. has been taken its place in the front ranks of the world.Antibiotic substitutes at present
The research and development of the aspects such as product probiotic bacteria, prebiotics and natural plant extracts are also by
Like a raging fire, achieve certain achievement at present.Along with livestock product safety is paid close attention to by the whole society
Further lifting, fermented feed raw material, the research of even complete feed fermentation and application
Become the main trend of industry development.But, the application technology of such as probiotics, antibacterial peptide
Production application technology etc. there is also many problems.Fermented feed refers under manual control condition,
The microorganism metabolic activity by self, by vegetalitas, animality and mineral material
Antinutritional factor is decomposed or converts, and produces the nutrient more can searched for food by poultry, digest, absorb
And the feedstuff of nonhazardous effect, fermented feed has and improves the palatability of feedstuff, stimulation
Poultry search for food, improve feedstuff Middle nutrition substance digestion rate and the function of utilization rate, are additionally beneficial to
Animal and bird intestines health and enhancing immunity, the noxious substance in degraded feedstuff, produce growth promotion
The factor, effectively escherichia coli, the Salmonella in suppression digestive tract and some are pathogenic carefully
The breeding of bacterium and pathogen and growth.
Creep feed is the feed product in piglets stage, be also piglets after birth to 4 week old this
A kind of special feed product containing high nutritional labeling in stage.High-quality creep feed at least should have
Standby being suitable to digests and alimentation；Good palatability；Comprehensive nutrition equalizes；Pig sickness rate is low；
Phenomenon without ablactation stress.And present creep feed usually contains many protein sources feedstuffs, cost is relatively
Height, therefore relatively low in the urgent need to a kind of cost, good palatability, the creep feed of religion groove excellent effect.
Summary of the invention
For solving the problems referred to above, it is an object of the invention to provide a kind of life for creep feed
Thing feedstuff and creep feed.
The biological feedstuff for creep feed that the present invention provides, by the feedstuff of water content 20-50%
Raw material is at a temperature of 15 DEG C-35 DEG C, and fermentation 3-10d prepares, and in described activated feed, lives
Bacterium number >=1 × 109Individual/g, containing organic acid >=2%, described feedstuff contains following weight proportioning
Component: yeast 0.05-0.2g/kg, lactic acid bacteria 0.05-0.2g/kg, bacillus cereus
0.05-0.2g/kg, expanded dehulled soybean meal 500-800g/kg, Semen Maydis powder 50-100g/kg, Semen Tritici aestivi
Bran 100-300g/kg.
In one embodiment of the invention, the described biological feedstuff for creep feed is by aqueous
The feedstuff of amount 30% is at a temperature of 30 DEG C, and sealing and fermenting 72 prepares, and described feedstuff is former
The material component containing following weight proportioning: yeast 0.1g/kg, lactic acid bacteria 0.1g/kg, spore
Bacillus 0.1g/kg, expanded dehulled soybean meal 525g/kg, Semen Maydis powder 75g/kg, Testa Tritici 225g/kg,
Water 175g/kg.
Wherein said yeast, lactic acid bacteria, the viable count of bacillus cereus is 20-30 hundred million
/g。
Wherein, described yeast is that saccharomyces cerevisiae CGMCC No.6560 (is disclosed in
CN201210430095.1), described bacillus cereus is bacillus subtilis CGMCC
No.8148, described lactic acid bacteria is Lactobacillus plantarum CGMCC No.8150.
Wherein, size-reduced before the fermentation of expanded dehulled soybean meal, grinding screen plate is 0.8 × 0.8mm,
Crush maize sieve is 0.8 × 0.8mm, and Testa Tritici grinding screen plate is 0.8 × 0.8mm.
The present invention also provides for a boar creep feed, with accounting for complete feedstuff in creep feed complete feedstuff
The biological feedstuff Peru Fish Dietary of 5%-10% mass percent is as protein raw material.
In a preferred embodiment of the present invention, described pig creep feed contains following weight proportion
Component: Semen Maydis 30%, popcorn 20%, bean cake 17%, sucrose 3%, glucose 3%,
Biological feedstuff 6%, fermented bean cake 8%, spray-dried plasma protein 6%, dalcium biphosphate 2%, stone
Powder 2%, fodder salt 1%, 98.5% lysine 0.3%, methionine 0.2%, tryptophan
0.01%, acidulant 0.5%, piglets premix material 1.5%.
In the present invention, described bacillus subtilis (Bacillus subtilis), Lactobacillus plantarum
(Lactobacillus plantarum) has good stomach juice-resistant, resistance to intestinal juice, bile tolerance (bud
Spore bacillus is high temperature resistant) etc. anti-adversity and produce acid, produce enzyme, the suppression prebiotic function such as pathogen,
Separate from healthy animal intestinal for applicant, selection-breeding obtains, and in JIUYUE in 2013 11 days
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, is called for short
CGMCC, address: the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 micro-life of the Chinese Academy of Sciences
Thing institute, deposit number is respectively as follows: CGMCC No.8148, CGMCC No.8150.
The bacillus subtilis CGMCC No.8148 of institute of the present invention selection-breeding is at artificial simulation gastric juices
Processing 3h in (pH2.0～4.0), survival rate is more than 65.8%；In artificial simulation intestinal juice
Processing 3h, survival rate is 74.7%；24h is processed at Fel Sus domestica saline solution (concentration 0.3～3g/kg),
Survival rate is more than 51.2%；85～100 DEG C process 3min, and survival rate is more than 11.2%；Right
Streptomycin, polymyxin B, 3 kinds of antibiosis of lincomycin have drug resistance；Can produce certain
The protease of amount, lipase, amylase, cellulase, xylanase, and effect is superior to
Bacillus subtilis CGMCC No.4628 used in application number 201110116641.X.
The Lactobacillus plantarum CGMCC No.8150 of institute of the present invention selection-breeding is at artificial simulation gastric juices
Processing 3h in (pH2.0～4.0), survival rate is more than 31.3%；In artificial simulation intestinal juice
Processing 3h, survival rate is 33.0%；24h is processed at Fel Sus domestica saline solution (concentration 0.3～3g/kg),
Survival rate is more than 3.7%；To cefepime, gentamycin, spectinomycin, streptomycin,
Polymyxin B, ciprofloxacin, 7 kinds of antibiosis of vancomycin have drug resistance；To escherichia coli
K88, K99 all have good inhibiting effect, antibacterial circle diameter be respectively 18.60mm,
15.43mm。
Technical scheme, has the advantage that
1, the lactic bacteria activity in the middle of the present invention is strong, can tolerate high temperature and sour environment, because of
This can suppress the growth of miscellaneous bacteria during the fermentation, and produces substantial amounts of organic acid and little peptide,
Improving the palatability of piglet, the most substantial amounts of viable bacteria can enter into the intestinal of piglet, son
The gastroenteric environment of pig plays antibacterial functions, improves the immunity of piglet, reduce piglet diarrhea
Rate, improves piglet survival ratio.
2, the yeast in the middle of the present invention can produce substantial amounts of vitamin B group and little peptide, carries
The immunocompetence of high piglet.
3, the bacillus cereus in the middle of the present invention, can produce substantial amounts of protease, amylase etc.,
The piglet digestibility to nutrient substance is improved after entering piglet gastrointestinal tract.
4, in the present invention, antinutritional factor is substantially reduced, and decreases the waste of feedstuff.
5, in the present invention, protein solubility improves, and improves the digestibility and utilization of true protein
Rate.
Detailed description of the invention
By the following example by the more specific description present invention, it should be understood that described enforcement
Example is merely to illustrate the present invention rather than limit the scope of the present invention by any way.
Embodiment 1 excellent species separation, screening, selection-breeding and qualification
1) strain expanding propagation: gather the fresh intestinal contents sample inoculation of healthy pig in LB
In fluid medium, postvaccinal culture medium cultivates expanding propagation 48h in 37 DEG C of calorstats；
2) bacillus subtilis is isolated and purified: to step 1) expanding propagation culture is at 80 DEG C of water
Bath heats 20min, then takes out treatment fluid, be diluted and coat LB and cultivate
In base, cultivating 24h in 37 DEG C of constant incubators, at picking from culture, each has spy
Levying the bacterium colony of form, more streak culture and separation, till the pure bacterium colony of Zhongdao, result is carried out
Gram stain microscopy, and will the doubtful pure colony inoculation separated to test tube slant expand
Numerous, then it is stored in 4 DEG C of refrigerators, standby；
Colonial morphology: bacterium colony rounding, smooth surface, is creamy white, size 1～3mm；Carefully
Born of the same parents' circle or oval, diameter 0.5～1.0um, great majority become short chains or right, Gram’s staining
Positive.
Identified, isolated bacterial strain is bacillus subtilis, and in JIUYUE in 2013 11 days
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center to be called for short
CGMCC, address: the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 micro-life of the Chinese Academy of Sciences
Thing institute, preserving number is CGMCC No.8148.
3) Lactobacillus plantarum is isolated and purified: to step 1) enriched medium doubling dilution,
Coat in MRS culture medium, under the conditions of 37 DEG C, cultivate 36～48h, therefrom each tool of picking
There is the bacterium colony of characteristic morphology the most streak culture and separate, until to pure bacterium colony, gram
Dyeing microscopic examination, breeds in the doubtful pure colony inoculation test tube slant that will separate, is then stored in 4 DEG C
In refrigerator, standby；
Colonial morphology: bacterium colony is soft and moistening, cheese color, glossy, smooth or slightly protruding,
Neat in edge；Cell is spherical or avette, diameter 5～10 μm, modes of reproduction budding.
Identified, isolated bacterial strain is Lactobacillus plantarum, and within 11st, protects in JIUYUE in 2013
It is hidden in China Committee for Culture Collection of Microorganisms's common micro-organisms center and is called for short CGMCC,
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica,
Preserving number is CGMCC No.8150.
The resistance of embodiment 22 strain excellent species and biology performance measure
2 strain strains: bacillus subtilis CGMCC No.8148 and Lactobacillus plantarum
The biology performance of CGMCC No.8150 and anti-adversity measure:
1) bacillus subtilis CGMCC No.8148 and Bacillus licheniformis CGMCC
The preparation of No.2383 culture: the slant strains of Storage in refrigerator is inoculated into LB seed culture
In base activate, 35 DEG C, 100rpm cultivate 16h, to obtain final product；
2) preparation of Lactobacillus plantarum CGMCC No.8150 culture: by Storage in refrigerator
Slant strains is inoculated in MRS seed culture medium activation, 30 DEG C, 150rpm cultivate 16h,
Obtain；
3) acid resistance measures: be inoculated in respectively by 10% inoculum concentration by the culture of above-mentioned preparation
In the artificial simulation gastric juices of pH value 2.0,3.0,4.0, again to adjust pH value be 2.0,3.0,
4.0,0h countings compare, and 3h sampling, is entered by 10 times of serial dilutions with 0.85% normal saline
Row viable plate count, calculates survival rate.
2 strain strains of selection-breeding of the present invention: bacillus subtilis CGMCC No.8148 and plant
Lactobacillus CGMCC No.8150 survival results in gastric acid is shown in Table 1.
The preparation of artificial simulation gastric juices: measure 9.5%～10.5% concentrated hydrochloric acid 16.4 milliliters, add steaming
Distilled water, to 1000 milliliters, does basis simulated gastric fluid, with hydrochloric acid or sodium hydroxide adjust pH value 2.0,
3.0,4.0, steam sterilization 15 minutes at 121 DEG C, add pepsin by 1% addition,
By 0.22 μm sterilised membrane filter, make simulated gastric fluid, take 5mL (4.5mL) and be sub-packed in examination
Guan Zhong.
Table 12 strain strain survival rate in artificial simulation gastric juices
4) resistance to intestinal juice measures: by 5% inoculum concentration, the culture of above-mentioned preparation is inoculated in people respectively
In work simulated intestinal fluid, 0h counting compares, and 3h sampling presses 10 times with the normal saline of 0.85%
Serial dilution, carries out viable plate count, calculates survival rate.
2 strain strains of selection-breeding of the present invention: bacillus subtilis CGMCC No.8148, plant
Lactobacillus CGMCC No.8150, and the Bacillus licheniformis CGMCC that laboratory preserves
No.2383 survival results in intestinal juice is shown in Table 2.
The preparation of artificial simulation intestinal juice: weigh 6.8g, adds distilled water to 1000 milliliters, does base
Plinth simulated intestinal fluid, steam sterilization 15 minutes at 121 DEG C, add Trypsin by 1% addition
Enzyme, by 0.22 μm sterilised membrane filter, makes simulated gastric fluid, takes 5mL (4.5mL) subpackage
In test tube.
Table 22 strain strain survival rate in artificial simulation intestinal juice
| Survival rate/% | |
| The process time | 3h |
| Bacillus subtilis CGMCC No.8148 | 74.7 |
| Lactobacillus plantarum CGMCC No.8150 | 33.0 |
5) bile tolerance measures: by the culture of 3 strain strains of above-mentioned preparation, by 10% inoculation
Amount is inoculated in the LB solution of 0.03%, 0.1%, 0.2%, 0.3% variable concentrations Fel Sus domestica salt respectively
In, 0h counting compares, and 24h samples with 0.85% normal saline based on 10 times of serial dilutions
Number, carries out viable plate count, calculates survival rate.
2 strain strains of institute of the present invention selection-breeding: bacillus subtilis CGMCC No.8148, plant
Thing lactobacillus CGMCC No.8150, and the Bacillus licheniformis CGMCC that laboratory preserves
No.2383 survival results in cholate is shown in Table 3.
The preparation of cholate: in the LB fluid medium prepared add 0.03%, 0.1%,
0.2%, the Fel Sus domestica salt of 0.3% variable concentrations, steam sterilization 30 minutes at 121 DEG C, aseptic
Under the conditions of, take the Fel Sus domestica salt of 5mL 0.03%, 0.1%, 0.2%, 0.3% variable concentrations respectively
Solution is sub-packed in test tube.
Table 32 strain strain processes the survival rate of 24h in variable concentrations Fel Sus domestica salt
6) the high temperature resistant mensuration of bacillus cereus: by the training of 2 bacillus strains of above-mentioned preparation
Support thing, respectively at 85 DEG C, 90 DEG C, 95 DEG C, 100 DEG C of water bath processing 3min, calculate survival
Rate.
Table 4 bacillus subtilis is at the survival rate/% of different high-temperature process 3min
7) drug: use quick paper disk method.
The drug result of table 52 strain strain
8) the product enzymatic determination of bacillus cereus:
Use flat board transparent circle method.Respectively cellulase culture medium, protease culture medium,
The spore that amylase culture medium, xylanase culture medium separate with dibbling in lipase culture medium
Bacillus, every kind of process sets 3 repetitions, cultivates 36h for 37 DEG C, and that observes flat board transparent circle has that it's too late
Size, if forming transparent bacterium colony or the circle of confusion in periphery of bacterial colonies, then proves bacillus cereus
Corresponding enzyme can be produced, measure transparent circle diameter (H) with slide gauge respectively and bacterium colony is straight
Footpath (C), calculates both ratios (H/C).By used in application number 201110116641.X
Bacillus subtilis CGMCC No.4628 as comparison, detection these 5 enzymes above simultaneously
H/C ratio.
The product enzymatic determination result of table 6 Bacillus subtilis strain
9) acidity test is produced:
Use calcium carbonate flat board that Lactobacillus plantarum CGMCC No.8150 is carried out production of organic acids
Measure；
The product acidity test result of table 7 Lactobacillus plantarum strain
10) bacteriostatic test: use Odontothrips loti that common pathogen is carried out bacteria inhibition assay.
A, in the test tube equipped with 10mL nutrient culture medium, activate two strain pathogenic bacterium:
K88, K99,37 DEG C of constant temperature culture 20h；
B, the preparation of double-layer plate: the flat board of cut-off footpath 90mm, inject the nutrition fine jade of sterilizing
Fat 15～20mL, horizontal positioned is allowed to solidify, and as bottom, separately takes Nutrient agar and (is cooled to 50 DEG C
Left and right) mixed with the instruction bacterium solution appropriate (50mL culture medium adds about 8mL) that 37 DEG C of 24h cultivate
Even, to draw 10mL and water in bottom culture medium, horizontal positioned is allowed to solidify, as bacterium
Layer；
C, add sample: grip sterilized Oxford cup with aseptic nipper, open ware lid, be placed on
In culture medium.Same amount of fermented liquid supernatant liquid (about 200uL) is filled it up with in the cup of Oxford, each
The repetition of 2, sample.The double dish adding sample are carefully placed in 37 DEG C of calorstats, cultivate
After 16～18h, take out and measure inhibition zone size.
The table 8 Lactobacillus plantarum antibacterial result to common pathogen
Embodiment 3 fermentation temperature and the screening of fermentation time
In laboratory conditions, the screening of biological feedstuff fermentation temperature has been carried out.Fermentation raw material
In contain: yeast 0.1g/kg, lactic acid bacteria 0.1g/kg, bacillus cereus 0.1g/kg, expanded go
Skin bean cake 525g/kg, Semen Maydis powder 75g/kg, Testa Tritici 225g/kg, water 175g/kg.Wherein
The fermentation of expanded dehulled soybean meal is front size-reduced, and grinding screen plate is 0.8 × 0.8mm, and crush maize sieves
Sheet is 0.8 × 0.8mm, and Testa Tritici grinding screen plate is 0.8 × 0.8mm.Take the 10 of mix homogeneously
Part each 200g of sample, is placed in big culture dish uniform lay, seals with adhesive tape afterwards,
Mold incubator is transferred to respectively 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C, at 35 DEG C, each
Putting two culture dishs under temperature conditions into and carry out fermentation culture, every day all observes entering of fermentation
Degree, if culture dish surface begins with aqueous vapor and produces, it was demonstrated that fermentation has begun to.From first
It starts, and takes out 5g sample every 24h and observes and measure.
In the result, along with laboratory temperature is from 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C
Raising, the required time that fermented is gradually shortened, 15 DEG C of 10d that need to ferment, and 20 DEG C need fermentation
8d, 25 DEG C of 5d that need to ferment, 30 DEG C of 3d that need to ferment, 35 DEG C of 3d that need to ferment.Due to factory's metaplasia
Produce, improve temperature and mean that raising energy expenditure, extend fermentation time and mean that reduction
Production efficiency, has therefore considered in many ways after factor, selects to ferment under the conditions of 30 DEG C
3d i.e. 72h is optimal conditions.
Embodiment 4 is fermented the screening of water content
Under factory conditions, the screening of water addition has been carried out.Fermentation raw material contains: yeast
Bacterium 0.1g/kg, lactic acid bacteria 0.1g/kg, bacillus cereus 0.1g/kg, expanded dehulled soybean meal 525g/kg,
Semen Maydis powder 75g/kg, Testa Tritici 225g/kg.The fermentation of the most expanded dehulled soybean meal is front size-reduced,
Grinding screen plate is 0.8 × 0.8mm, and crush maize sieve is 0.8 × 0.8mm, and Testa Tritici is pulverized
Sieve is 0.8 × 0.8mm.Take 14 Plastic Drums, by the dry material moisture of mix homogeneously according to
12% calculates, and adds tap water so that it is water content respectively reaches 20%, and 25%, 30%,
35%, 40%, 45%, 50%, each water content loads two buckets, as parallel group.
After filling, compacting seals, and ferment under the conditions of 30 DEG C 72h.
Finding in the result, along with the continuous rising of water content, ferment effect is become better and better,
But be as the rising of water content, the mobility of biological feedstuff the most worse and worse, poor fluidity
Mean that and cannot add in complete feedstuff.Therefore mobility and ferment effect have been considered
Afterwards, showing that water content 30% group is best, the percentage ratio i.e. adding water is 25%.
The biological feedstuff that embodiment 5 present invention produces in optimal conditions measures
Under factory conditions, the optimum condition temperature in screening is 30 DEG C, water content 30%
Under the conditions of with biological feedstuff fermentation bag ferment 72h, wherein fermentation raw material contains: yeast
0.1g/kg, lactic acid bacteria 0.1g/kg, bacillus cereus 0.1g/kg, expanded dehulled soybean meal 525g/kg,
Semen Maydis powder 75g/kg, Testa Tritici 225g/kg.The fermentation of the most expanded dehulled soybean meal is front size-reduced,
Grinding screen plate is 0.8 × 0.8mm, and crush maize sieve is 0.8 × 0.8mm, and Testa Tritici is pulverized
Sieve is 0.8 × 0.8mm.Take three parts of parallel sample after having fermented and deliver to Beijing great Bei agriculture life
Thing feedstuff inspection center is detected, and testing result is as shown in table 9.
The biological feedstuff testing result that table 9 produces in optimal conditions
Testing result shows, wherein, in tunning, containing viable count >=1 × 109Individual/g,
Containing organic acid >=2%
The feeding effect that embodiment 6 present invention produces for creep feed
Materials and methods
Test specimen: the biological feedstuff of the embodiment of the present invention 5 preparation with 6% is prepared without fish flour
Creep feed.
Matched group: Semen Maydis 30%, popcorn 20%, bean cake 17%, sucrose 3%, Fructus Vitis viniferae
Sugar 3%, super imported fish meal 6%, fermented bean cake 8%, spray-dried plasma protein 6%, di(2-ethylhexyl)phosphate
Hydrogen calcium 2%, stone powder 2%, fodder salt 1%, 98.5% lysine 0.3%, methionine 0.2%,
Tryptophan 0.01%, acidulant 0.5%, big north agriculture piglets premix material 1.5%.
Test group: Semen Maydis 30%, popcorn 20%, bean cake 17%, sucrose 3%, Fructus Vitis viniferae
Sugar 3%, embodiment 5 preparation biological feedstuff 6%, fermented bean cake 8%, spray-dried plasma protein 6%,
Dalcium biphosphate 2%, stone powder 2%, fodder salt 1%, 98.5% lysine 0.3%, egg ammonia
Acid 0.2%, tryptophan 0.01%, acidulant 0.5%, big north agriculture piglets premix material 1.5%.
Daily ration uses cold granulation mode to produce.
Test period: 7 days-November 28 November in 2014
EXPERIMENTAL DESIGN:
Select DLY piglet 24 nest of 1 week old, use one factor factorial experiment with complete randomization design design,
It is divided into two process groups, often 12 repetitions of group, each repetition piglet 8-12 head.Original body mass
Statistical analysis, group difference is not notable (P > 0.05).
Feeding and management
Piglet begins from birth on the 7th day, starts to feed creep feed, hand fed, feeds many meal less,
Freely drink water, free choice feeding, temperature 26 DEG C, observe the diarrhoea status of piglet every morning,
Calculating feed intake, stay 7 days after 21 age in days wean on delivery bed, off-test, in test
At the end of weigh.
Test determination index: to be repeated as unit, record litter weight, dead number of elements, diarrhoea are only
Number, feed consumption rate etc., calculate average weight, daily material consumption, mortality rate, feedstuff-meat ratio etc..
Statistical analysis
Use statistic software SPSS 13.0 software that test data is carried out statistical analysis.
2 result of the tests are shown in Table 10.
As can be seen from Table 10: test group is compared with matched group, from on-test to the 21st
It age in days piglet daily gain and feed intake are without significant difference (P > 0.05).At 7 ages in days-the 28
Age in days growth stage, test group piglet feed intake and daily gain are above matched group, but difference
Not notable (P > 0.05).Piglet is at each stage diarrhea rate significant difference (P < 0.05), mortality rate
Significant difference (P < 0.05).
3 conclusions
Result of the test shows, the creep feed produced with the biological feedstuff of the present invention is balanced in nutrition,
Digestibility is high, can the effective expensive animal and vegetable protein raw material such as Peru Fish Dietary, and
Nest counterpoise is noticeably greater than matched group, significantly reduces diarrhea rate and the mortality rate of piglet, it is ensured that young
Pig health fast-growth.
Table 10 present invention produces the impact on weaned piglets for creep feed
| Project | Matched group | Test group | P value |
| 7 ages in days nest counterpoise (kg) | 23.6 | 22.8 | P>0.05 |
| 28 ages in days nest counterpoise (kg) | 57.6 | 65.9 | P<0.05 |
| The equal feed consumption rate of nest (kg) | 19.64 | 24.13 | P<0.05 |
| Diarrhea rate (%) | 6.01 | 2.39 | P ＜ 0.05 |
| Mortality rate (%) | 1.9 | 0.72 | P<0.05 |
The above is only the preferred embodiment of the present invention, it is noted that for this technology
For the those of ordinary skill in field, on the premise of without departing from the technology of the present invention principle, also
Can make some improvements and modifications, these improvements and modifications also should be regarded as the protection of the present invention
Scope.
Claims (7)
1. the biological feedstuff for creep feed, it is characterised in that by water content 20-50%
Feedstuff at a temperature of 15 DEG C-35 DEG C, sealing and fermenting 3-10d prepare, described biology
In feedstuff, viable count >=1 × 109Individual/g, containing organic acid >=2%, under described feedstuff contains
State the component of weight proportion: yeast 0.05-0.2g/kg, lactic acid bacteria 0.05-0.2g/kg, spore
Bacillus 0.05-0.2g/kg, expanded dehulled soybean meal 500-800g/kg, Semen Maydis powder 50-100g/kg,
Testa Tritici 100-300g/kg.
2. biological feedstuff as claimed in claim 1, it is characterised in that by water content 30%
Feedstuff at a temperature of 30 DEG C, sealing and fermenting 72h prepare, described feedstuff contains
There is a component of following weight proportioning: yeast 0.1g/kg, lactic acid bacteria 0.1g/kg, bacillus cereus
0.1g/kg, expanded dehulled soybean meal 525g/kg, Semen Maydis powder 75g/kg, Testa Tritici 225g/kg.
3. biological feedstuff as claimed in claim 1 or 2, it is characterised in that described ferment
Female bacterium, lactic acid bacteria, the viable count of bacillus cereus is hundred million/g of 20-30.
4. biological feedstuff as claimed in claim 3, it is characterised in that described yeast
For saccharomyces cerevisiae CGMCC No.6560, described bacillus cereus is bacillus subtilis
CGMCC No.8148, described lactic acid bacteria is Lactobacillus plantarum CGMCC No.8150.
5. biological feedstuff as claimed in claim 1 or 2, it is characterised in that expanded peeling
Before bean pulp fermentation size-reduced, grinding screen plate is 0.8 × 0.8mm, crush maize sieve is 0.8 ×
0.8mm, Testa Tritici grinding screen plate is 0.8 × 0.8mm.
6. a boar creep feed, it is characterised in that with accounting for complete feedstuff in creep feed complete feedstuff
The biological feedstuff Peru Fish Dietary described in any one of claim 1-5 of 5%-10% is as protein
Raw material.
7. pig creep feed as claimed in claim 6, it is characterised in that containing following weight
The component of proportioning: Semen Maydis 30%, popcorn 20%, bean cake 17%, sucrose 3%, Fructus Vitis viniferae
Sugar 3%, biological feedstuff 6%, fermented bean cake 8%, spray-dried plasma protein 6%, dalcium biphosphate
2%, stone powder 2%, fodder salt 1%, 98.5% lysine 0.3%, methionine 0.2%, color
Propylhomoserin 0.01%, acidulant 0.5%, piglets premix material 1.5%.
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