CN106721021A - A kind of piglet antibiotic-free feed and preparation method thereof - Google Patents
A kind of piglet antibiotic-free feed and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of piglet antibiotic-free feed and preparation method thereof, belong to Feeding Prescription Design and processing technology field, be mainly prepared from the following raw materials in parts by weight：50 60 parts of corn, 24 parts of steam fish meal, 10 16 parts of dregs of beans, 15 parts of expanded soybean, 35 parts of flour, whey powder 36, coconut oil 0.5 1, soybean oil 0.5 1, antibacterial peptide 0.02 0.03, probiotics 13,0.012 part of yucca extract, 0.3 0.7 parts of stone flour, 0.3 0.5 parts of salt, 0.8 1.2 parts of calcium monohydrogen phosphate, 0.3 0.8 parts of amino acid, 0.03 0.05 parts of complex enzyme, 0.3 0.4 parts of B B-complex, 0.2 0.6 parts of composite trace element；The present invention comprehensively solves problem above from piglet nonreactive diet nutrient standard, using the measure substitute antibiotics such as antibacterial peptide by adding the aspects such as probiotics, and daily gain improves 6.17%, and average daily feed intake improves 5.38%, diarrhea rate reduction by 8.72%.Intestine of young pigs health status is good, and premunition is strong, is early to deliver for sale, not medication, provide safe meat and establish early stage basis.
Description
Technical field
The invention belongs to Feeding Prescription Design and feed processing technology field, more particularly to a kind of piglet antibiotic-free
Feed, and it is related to the preparation method of the feed.
Background technology
With the generation of feed particularly mixed feed, make it possible scale, the pig-breeding of modernization, and raising
Some feed addictives, such as the trace element needed for adding pig growth etc. are added in material, growing for live pig is played
Positive effect, can improve the daily gain of live pig, shorten the marketing time of live pig, reduce the feeding cost of live pig, obtain
Preferable economic benefit, grows so as to bring the fast-developing of hog feeding, also makes the meat product in people's daily life
Sound assurance can be obtained.However, in feeding process of live pig, in order that live pig can obtain certain disease-resistant by food
Ability, generally requires to add antibioticses additive in feed, and although the addition of these compositions improves pork total output, so
And side effect of the antibiotic to growth of animal and products thereof quality mainly includes：Make it is bacterial resistance occurred, cause livestock and poultry body
Immunity degradation, cause livestock and poultry autogenous infection and suprainfection, cause meat, eggs and milk quality decline, can in livestock products and environment
In cause residual etc., directly threaten human health and safety.
Solving pig health problem must rely on aggregate measures, continue to strengthen the vaccine and medication tube with the preventing and treating of specification swine disease
While reason, seek new approaches, study and apply new theory, new technology, new product particularly significant and necessity.Modern medicine and life
Thing research shows that nutrition is the key factor for determining health.Nutrition is the material base of all life activity, both influences animal
Productive potentialities and efficiency, also determine animal health condition.
Publication number:The Chinese invention of 103750073A《It is a kind of strengthen live pig immunity improve meat quality pig feed and
Its preparation method》Disclose a kind of live pig immunity that strengthens and improve pig feed of meat quality and preparation method thereof, it is mainly
It is prepared from according to a certain weight ratio by corn, dregs of beans, wheat bran, folium isatidis, motherwort, balsam pear, common nepenthes etc., the present invention has
The nutriments such as rich in protein, vitamin, amino acid and clearing heat and detoxicating, the function of being healthy and strong of nourshing kidney moistening lung, according to quantity collocation are raised
Supporting can not only strengthen live pig immunity, and also improve the quality and nutritive value of pork.
Publication number:The Chinese invention of 103404730A《A kind of formula of piglet feed containing yucca extract》Disclose one kind
Formula of piglet feed containing yucca extract；Mainly mixed by the material of following weight parts：35~65 parts of corn flour, sorghum
15~30 parts of powder, 15~30 parts of dregs of beans, 10~20 parts of vinasse, wheat wheat-middlings 10~20,5~15 parts of wheat bran, yucca extract 5~
20 parts, 1~8 part of salt；The invention is used for feeding piglet, can strengthen pigling immunity, improves its speed of growth.But its formula is not
It is enough scientific and reasonable, it is impossible to meet and with the addition of terramycin in the nutritional need of piglet, and the invention.
Publication number:The Chinese invention of 104945484A《A kind of antibacterial peptide and preparation method and application》Disclose a kind of people
The active antibacterial polypeptide of work design synthesis, as shown in SEQIDNO.1, its molecular weight is 1483.88Da to its amino acid sequence, waits electricity
Point is 10.30.The antibacterial peptide is applied in pig starter feed, is mainly used in antibacterial, raising weanling pig daily gain and feed intake, drop
Low feed-weight ratio, reduction diarrhea of weaned piglets rate, and improve weanling pig function of intestinal canal.Compared with prior art, it is of the invention
Antibacterial peptide has that small molecular weight, artificial synthesized convenience, bactericidal action be strong, the low advantage of cytotoxicity, is applied in pig starter feed
Intestine of young pigs health can be significantly improved, the growth performance of piglet is improved.
Publication number:The Chinese invention of 105325695A《A kind of preparation side of anti diar rhea Chinese herbal medicine fermented type the feed for piglet
Method》A kind of preparation method of anti diar rhea Chinese herbal medicine fermented type the feed for piglet is disclosed, belongs to feed and microbial fermentation technology
Field.The present invention in proportion mixes hawthorn, the Radix Astragali, dried orange peel, Radix Codonopsis, Asiatic plantain, dark plum, honeysuckle, corn flour, dregs of beans with water
Water is sized mixing and is made fermentation medium, the immersion that heats up, sterilizing, and accesses producing enzyme bacillus licheniformis, producing enzyme when cooling to 38-40 DEG C
Aspergillus niger and condensation lactic acid bacillus, after fermentation 24-108h, drying is made anti diar rhea Chinese herbal medicine fermented type the feed for piglet.
The feed is rich in anti diar rhea Chinese herbal medicine, small peptide and lactic acid, with nutrition is high, easy to digest, good palatability, ANFs content
It is low, the features such as growth promotion performance is good.There is action come preventing piglet diarrhea in the main Chinese medicine by particular combination of fermenting of the invention
Hurry up, relatively costly shortcoming.
Publication number:The Chinese invention of 103719585A《A kind of pig starter feed》A kind of pig starter feed is disclosed, mainly under
The raw material of row weight portion is made：Corn flour 10-18 parts, dregs of beans 8-15 parts, fish meal 5-10 parts, oyster shell whiting 1-8 parts, wheat bran 10-20
Part, saccharin sodium 0.5-2 parts, 1-10 parts of class emulsion, microorganism formulation 1-5 parts, 0.1-1 parts, salt 0.1-1 parts of trace element.Raise
Fish meal in material can meet nutritional need of the piglet to animal protein, and oyster shell whiting can provide the calcium source of abundance for piglet.
Dusty yeast, bacillus subtilis powder and low shitosan are used cooperatively, and can adjust probiotics flora in piglet intestines body, strengthen piglet
Autoimmunity, reduce disease evil generation；Saccharin sodium provides the sweet taste of abundance, and the class breast for providing newborn taste for pig starter feed
Agent is used cooperatively, and the taste of similar breast milk, induction piglet feed are provided for piglet.Invention formula is excessively simple, and composition is inadequate
Rationally, nutrition is unbalanced, it is impossible to meet the nutritional need of piglet.
Publication number:The Chinese invention of 101999560A《A kind of sour power the feed for piglet of low system and preparation method thereof》Disclose
A kind of preparation of sour power the feed for piglet of low system and production method, using corn, dregs of beans, fermented bean dregs, whey powder, vitamin and
Trace element etc. is made up as raw material of the special processing mode such as low temperature granulation after expanded, crushing；The present invention changes routine
The shortcoming of the sour power of the feed for piglet system high, reduces the situation of the low digestibility of suckling piglet, improves feed palatability, improves breast young
Pig intestinal health, improves suckling piglet Growth Results.The invention complicated component, preparation is difficult, and nutrition is unbalanced, is unfavorable for promoting and answers
With.
Feed has different advantageous features described in above-mentioned patent document, and the present invention is according to piglet nonreactive diet nutrient
Standard, using the measure substitute antibiotics such as antibacterial peptide, the advantage of good palatability high with Amino Acid Absorption utilization rate；By adding
Plus probiotics etc., it is ensured that intestine of young pigs health, strengthen premunition, it is early to deliver for sale, not medication, safe meat is provided establishes early stage base
Plinth.
The content of the invention
The present invention designs disease-resistant nutritive standard, by adding antibacterial peptide, yucca extract, benefit with piglet as research object
The aspects such as raw bacterium design Diet Formula, it is ensured that intestine of young pigs health, strengthen premunition.
The technical solution adopted by the present invention is：A kind of piglet antibiotic-free feed, is prepared from the following raw materials in parts by weight：
Corn 50-60 parts, steam fish meal 2-4 parts, dregs of beans 10-16 parts, expanded soybean 1-5 parts, flour 3-5 parts, whey powder 3-6, coconut
Oily 0.5-1, soybean oil 0.5-1, antibacterial peptide 0.02-0.03, probiotics 1-3,0.012 part of yucca extract, stone flour 0.3-0.7
Part, salt 0.3-0.5 parts, calcium monohydrogen phosphate 0.8-1.2 parts, amino acid 0.3-0.8 parts, complex enzyme 0.03-0.05 parts, it is combined dimension life
It is plain 0.3-0.4 parts, composite trace element 0.2-0.6 parts；
Further, the complex enzyme composition is：Cellulase 1, protease 2, AMS 2；
Further, the B B-complex composition is：Calciferol .5-6 parts, biotin 0.8-1.2 parts, folic acid 1-2
Part；
Further, the composite trace element composition is：Yeast selenium 1-2 parts, zinc oxide 1-2 parts.
The piglet antibiotic-free feed：Net energy 2450Kcal/kg, protein content 18%.
The piglet antibiotic-free feed：Ideal amino acid ratio Lys under the conditions of disease-resistant:(Met+Cys):Thr:Trp:
Arg:Val=100:65:77:22:108:90.
The yucca extract is provided by the industrial Science and Technology Ltd. of occasion of Beijing China.
The piglet antibiotic-free feed in：After operational phase was for wean two weeks --- the piglet of 15kg.
The probiotics is preferably the saccharomyces cerevisiae (Saccharomyces of deposit number CGMCC No.12789
Cerevisiae) tlj2016,；
Preferably, the saccharomycete cultivates preparation by the following method：
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed
Culture 30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Seed liquor is pressed into 10% mass percent inoculum concentration, is accessed in the fermentation tank equipped with 3L fermentation mediums, 30 DEG C, led to
Tolerance 6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably add
Plus the Cys of final concentration of 25mmol/L, total fermentation time is 50h；
Fermentation is finished, and pH is to 6.5 for regulation, is spray-dried to obtain yeast bacteria powder；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0。
Present invention simultaneously provides a kind of preparation method of piglet antibiotic-free feed, comprise the following steps：By formula, by jade
Rice and dregs of beans first mix, and size-reduced machine is crushed, grinding screen plate aperture 1.0mm, then quenched 1min, are steamed in 0.272-0.275Mpa
Steam pressure with 80 degree under the conditions of product is cured, then will curing after raw material mix with other raw materials, under the conditions of 65 degree system
Grain.
Beneficial effect：
From piglet nonreactive diet nutrient standard, using the measure substitute antibiotics such as antibacterial peptide, by adding the side such as probiotics
Face comprehensively solves problem above, and daily gain improves 6.17%, and average daily feed intake improves 5.38%, diarrhea rate reduction by 8.72%.It is young
Chitling road health status is good, and premunition is strong, is early to deliver for sale, not medication, provide safe meat and establish early stage basis.
The present invention uses nonreactive mixed feed, and corn carries out decortication treatment, using expanded soybean, both remains cereal
Original flavor, the digestibility of nutriment therein is improve again；Without addition antibiotic, therefore, the present invention is for pig
Health, Pork Security, back to nature, green consumption are significant.Particularly add the preservation of bacteria strain of high yield glutathione
Tlj2016, glutathione is such as combined as important in vivo antioxidant and free radical scavenger with free radical, heavy metal,
So as to the poisonous substance being harmful in body is converted into harmless material, excrete in vitro, the physical function to improving sucking pig is very beneficial
Place；
Biotin and folic acid promote IFN-γ synthesis, alleviate destructions of the PCV2 to pig lymphoid tissue, promote immune response anti-
Should be with PCV2 antibody tormations, incidence of disease reduction by 10%.Vitamin D is by promoting piglet rotavirus (RV) antibody and intestinal mucosa
Secretory immunoglobulin A (SIgA) is generated, the key molecule expression of regulation RIG-1 signal paths, is reduced RV and is attacked bearing chitling
Mucous membrane inflammatory reaction, significantly reduces diarrhea rate.Tryptophan can promote grower pigs IGF-1 to secrete and specific antibody synthesis, delay
The growth inhibition that Xie Yin inoculation PRRSV and PRV Attenuate vaccines cause.Threonine by strengthen lymphocyte function, promote it is special and
Non-specific antibody is produced, lowers TLR expression, reducing inflammatory factor generation and promote the approach such as protein synthesis to alleviate PRV
Damage to piglet.Arginine can lower piglet TLR4 and TLR5 and its expression of signal pathway key molecule, alleviate Salmonella bar
Activation of the bacterium to immune system.
Under oxidative stress situation, the metabolism of piglet selenium and requirement change, and the selenium addition of 0.2mol/kg can not
Meet the oxidation resistant needs of antibody；The mechanism of selenium anti-oxidation stress may be with influence trilute (T3), thyroid gland
Secretion, the regulation β of cytokine interleukin -1 (1L-1 β) and the generation of interleukin-6 (1L-6) of plain (T4) and
NF-kB activity is relevant.Selenium source and selenium level have mediating effect+6 to piglet growth performance and immunologic function, so that it is determined that piglet is obtained
Obtain the selenium source and selenium level of optimal immunoprotective effec.Research discovery to zinc, the piglet zinc requirement foot that NRC (1998) recommends
To meet under normal condition the need for piglet growth and appropriate immune power, but need to greatly improve zinc under the conditions of immunological stress
Addition.
Antibacterial peptide is widely present in bacterium, plant, amphibian, mollusk, fish, birds and mammal body
Small molecule polypeptide.
Surface tension can be eliminated in yucca extract composition, improves intestinal pH, improve small intestinal mucosa cell permeability, can
To improve the emulsifying capacity to nutriment, especially fat and liposoluble substance absorptivity, so as to improve efficiency of feed utilization
And livestock and poultry production performance.
Probiotics can not only by improve intestinal microecology balance promote body health, and can reduce breeding environment and
The content of the harmful substance such as ammonia, hydrogen sulfide, organophosphor in excrement, reduces animal husbandry pollution on the environment, protecting ecology
Environment, with obvious economic benefit and social benefit.
Specific embodiment
The Wine brewing yeast strain is specially saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, the bacterium
Strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number on July 15th, 2016
It is CGMCC No.12789, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode 100101.
The most suitable growth pH of the saccharomyces cerevisiae tlj2016 is 6.0-6.5, and optimum growth temperature is 28-35 DEG C；
The saccharomyces cerevisiae tlj2016 is isolated saccharomyces cerevisiae starting strain from the orchard of one plant of Ningxia through following
Step is obtained：
The original strain that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → hypertonic plate screening → nitrosoguanidine
(NTG) secondary screening (producing glutathione GSH abilities) → passage stabilization is screened → fermented to mutagenesis screening → hypertonic flat board primary dcreening operation → shaking flask
Property experiment.
By the bacterial strain tlj2016 obtained after mutagenesis, its glucose tolerance and Cys tolerance are equal
It is improved, cell density, another aspect Cys tolerance energy on the one hand can be improved under high concentration glucose culture
The raising of power is beneficial to GSH and largely synthesizes in intracellular, so as to improve the ability that bacterial strain mass produces GSH.
Saccharomyces cerevisiae of the present invention reaches 300g/L to the tolerance of glucose, beneficial to it in high concentration grape
GSH is produced under the conditions of sugar；3308mg/L is reached in 5L fermentation cylinder for fermentation production GSH final concentrations；Tolerate the energy of Cys
Power is far above starting strain, slow growth is remained under the effect of 5mmol/L Cys, in 40mmol/LL- cysteines
Remain to keep GSH largely to synthesize under effect；Salt resistance ability reaches 18%, is conducive to extending its application field.
1.DES mutagenic and breedings
1) ring of starting strain 1 one on test tube slant is taken on super-clean bench, is accessed equipped with 50mL malt extract mediums
In 250mL triangular flasks, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.
2) 5mL bacterium solutions are taken, 5000rpm centrifugation 10min collects thallines, with brine 2 times.
3) 10 are diluted to pH7.0 phosphate buffers7Individual/mL bacteria suspensions.
4) kaliumphosphate buffer of 32mL pH7.0,8mL bacteria suspensions, 0.4mL DES are taken and is being placed in advance in the 150mL of rotor
It is sufficiently mixed in triangular flask, makes DES ultimate densities be 1% (v/v).
5) the 150rpm reactions 30min in 30 DEG C of shaking tables, takes 1mL mixed liquors, adds 0.5mL 25%Na2S2O3In solution
Only react.
6) dilution spread is in the malt extract medium plate containing 150g/L KCl, the picking bacterium after 30 DEG C of cultures 2-3 days
The bacterial strain 1 of the maximum that falls.
2. nitrosoguanidine mutagenesis
1) ring of saccharomyces cerevisiae bacteria strain 1 one on test tube slant is taken on super-clean bench, is accessed and 50mL brewer's wort cultures is housed
In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.
2) 5mL bacterium solutions 5000rpm centrifugation 10min collects thallines are taken, with brine 2 times.
3) 10 are diluted to pH6.0 phosphate buffers7Individual/mL bacteria suspensions.
4) 10mL bacteria suspensions are taken to be transferred in 100mL triangular flasks, the NTG of 10mg is added, final concentration of 10mg/mL is configured to
NTG solution, and add 4-5 drop acetone, be beneficial to NTG dissolvings.
5) the 200rpm oscillating reactions 30min at 30 DEG C, 5000rpm are centrifuged 10min collects thallines, use SPSS
Wash for several times, stopped reaction.
6) appropriate dilution spread, takes the bacterium solution 0.2mL of last dilution factor, coats the brewer's wort culture containing 200g/L KCl
In base plate.Picking colony 20 after being cultivated 2-3 days at 30 DEG C.
3. shaking flask primary dcreening operation
1) take above-mentioned each ring of 20 S. cervisiaes respectively on super-clean bench, be respectively connected to equipped with 50mL brewer's wort cultures
In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 12h or so, thalline is in mid log phase.
2) 5mL bacterium solutions are taken, is accessed equipped with the 250mL in the hypertonic malt extract mediums of 50mL (concentration of glucose is 300g/L)
In triangular flask, 200rpm, 30 DEG C are cultivated 3-4 days, daily detection concentration of glucose and concentration of alcohol change.After fermentation ends, than
Compared with 20 plants of glucose and ethanol wear rate of strain, final remaining sugar concentration and concentration of alcohol, glucose to the conversion ratio of ethanol
And heteroacid content.
3) glucose consumption rate is fast, final remaining sugar concentration is low and concentration of alcohol is high 5 plants of bacterium are named as Y-1, Y- for selection
2, Y-3, Y-4, Y-5.
4. ferment secondary screening
By 5 plants of bacterium Y-1, Y-2, Y-3, Y-4, Y-5 and starting strain of gained in step 3, respectively according to 10% mass
Fraction inoculum concentration, the 150rpm in the 250mL shaking flasks equipped with 30mL fluid nutrient mediums, 30 DEG C of culture 30h, takes zymotic fluid measure
GSH concentration；
Fluid nutrient medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1；
GSH assay methods：Fresh yeast, at 30 DEG C, 40% Ethanol Treatment 3h, in centrifuging and taking are obtained after zymotic fluid centrifuge washing
Clear liquid is cooked GSH determination samples；
GSH measure is carried out using alloxan method：Its principle is that-the SH on GSH reacts with alloxan, the material of generation
There is absworption peak at 305nm, and it is linear with glutathione concentrations, therefore can be quantitative determined with ultraviolet specrophotometer
GSH contents.
Table 1：GSH testing results
| Bacterial strain | Starting strain | Y-1 | Y-2 | Y-3 | Y-4 | Y-5 |
| GSH concentration (mg/L) | 127.9 | 195.7 | 172.3 | 263.2 | 216.5 | 185.2 |
As seen from the results in Table 1, bacterial strain Y-3 has highest GSH fermentabilities, it is thus determined that Y-3 is final production bacterium
Strain, and it is named as tlj2016.
5. genetic stability experiment
Continuous ten passages on inclined-plane by tlj2016 bacterium, and detect the hair after passage every time with the method for shaking flask secondary screening
Ferment situation.Experiment finds that continuous ten passages on inclined-plane, the strain proterties does not have significant change, and property indices are all just
Often, illustrate that the genetic stability of the strain is stronger.
Fermented under the conditions of saccharomyces cerevisiae tlj2016 sugar high and produce GSH capacity experimentals
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed
Culture 30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Seed liquor is pressed into 10% mass fraction inoculum concentration, is accessed in the fermentation tank equipped with 3L fermentation mediums, 30 DEG C, ventilated
Amount 6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposable addition
The Cys of final concentration of 25mmol/L, total fermentation time is 50h；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0；
After fermentation ends, the content for determining GSH in zymotic fluid is 3308mg/L.
Saccharomyces cerevisiae tlj2016L- cysteines tolerance is tested
By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to
150rpm in shaking flask, 30 DEG C are cultivated, when culture is to 12h, to the Guang ammonia of L- half that different final concentrations are added in shaking flask
Acid, is further cultured for 10h, determines dry cell weight, as a result table 2,3；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 20, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
Table 2：Starting strain Cys tolerance
The tlj2016L- cysteine tolerances of table 3
| Cys concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
| Tlj2016 dry weights g/L | 25.7 | 28.5 | 23.6 | 21.2 | 20.6 | 18.7 |
| GSH concentration (mg/L) | 73.2 | 98.3 | 113.5 | 121.7 | 127.5 | 135.8 |
From the results shown in Table 2, for starting strain, Cys are added in culture medium, cell stops growing,
And start self-dissolving, cause GSH growth rates to be reduced with the rising of Cys concentration；From the results shown in Table 3,
Under low concentration Cys, tlj2016 still is able to slow growth, with the raising of Cys concentration, tlj2016 bacterial strains
Dry cell weight slowly decline, and GSH concentration sustainable growths, before this result is beneficial in GSH production processes by addition
Body amino acid-Cys promote the production of GSH.
Saccharomyces cerevisiae tlj2016 salt resistance abilities are tested
Take tlj2016 bacterium solutions 1mL inoculation strain in containing different NaCl concentrations (concentration gradients be 0%, 2%, 5%,
10%th, 15%, 10mL YPD fluid nutrient mediums (pH=6.5) 18%), 24h is cultivated at being placed in 30 DEG C respectively, each treatment 3
Individual repetition.Respectively take 1ml samples bacterium solution to be mixed in 9ml physiological saline, prepare dilution factor solution, take 0.1ml dilutions solid in YPD
It is coated with body flat board, culture 36 hours (each dilution factor do 3 parallel) record is inverted in 30 DEG C of biochemical cultivation cases and calculates flat
Bacterium number number on plate.The results are shown in Table 4, it is known that the resistance to salinity of the bacterium is 18%, illustrates that tlj2016 not only can be in conventional ring
Survived in border, still there is vigor under high salt conditions, can be applied to consume sugar in the high salt food processing process such as soy sauce, curing food
Produce glutathione.
The salt resistance ability of table 4 detection (× 107cfu/ml)
Embodiment 1
A kind of piglet antibiotic-free feed, is prepared from the following raw materials in parts by weight：
55 parts of corn, 3 parts of steam fish meal, 13 parts of dregs of beans, 3 parts of expanded soybean, 4 parts of flour, whey powder 4, coconut oil 0.5,
Soybean oil 1, antibacterial peptide 0.02,2 parts of probiotics, 0.012 part of yucca extract, 0.5 part of stone flour, 0.4 part of salt, calcium monohydrogen phosphate
1.0 parts, 0.6 part of amino acid, 0.04 part of complex enzyme, 0.35 part of B B-complex, 0.4 part of composite trace element；
The piglet antibiotic-free feed：Net energy 2450Kcal/kg, protein content 18%.
The piglet antibiotic-free feed：Ideal amino acid ratio Lys under the conditions of disease-resistant:(Met+Cys):Thr:Trp:
Arg:Val=100:65:77:22:108:90.
The complex enzyme is constituted：Cellulase 1, protease 2, AMS 2；
The B B-complex is constituted：4 parts of vitamin D, 1 part of biotin, 1 part of folic acid；
The composite trace element is constituted：2 parts of yeast selenium, 1 part of zinc oxide.
The yucca extract is provided by the industrial Science and Technology Ltd. of occasion of Beijing China.
The piglet antibiotic-free feed in：After operational phase was for wean two weeks --- the piglet of 15kg.
The probiotics is saccharomycete；
The saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and preservation is compiled
Number be CGMCC No.12789；
The saccharomycete cultivates preparation by the following method：
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed
Culture 30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Seed liquor is pressed into 10% inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, throughput 6L/
Min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably add final concentration
It is the Cys of 25mmol/L, total fermentation time is 50h；
Fermentation is finished, and pH is to 6.5 for regulation, is spray-dried to obtain yeast bacteria powder；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0。
Present invention simultaneously provides a kind of preparation method of piglet antibiotic-free feed, comprise the following steps：By formula, by jade
Rice and dregs of beans first mix, and size-reduced machine is crushed, grinding screen plate aperture 1.0mm, then quenched 1min, are steamed in 0.272-0.275Mpa
Steam pressure with 80 degree under the conditions of product is cured, then will curing after raw material mix with other raw materials, under the conditions of 65 degree system
Grain.
Embodiment 2
A kind of piglet antibiotic-free feed, is prepared from the following raw materials in parts by weight：
50 parts of corn, 4 parts of steam fish meal, 16 parts of dregs of beans, 1 part of expanded soybean, 5 parts of flour, whey powder 3, coconut oil 1, greatly
Soya-bean oil 0.5, antibacterial peptide 0.03, probiotics 1,0.012 part of yucca extract, 0.7 part of stone flour, 0.3 part of salt, calcium monohydrogen phosphate 0.8
Part, 0.3 part of amino acid, 0.05 part of complex enzyme, 0.4 part of B B-complex, 0.2 part of composite trace element；
The piglet antibiotic-free feed：Net energy 2450Kcal/kg, protein content 18%.
The piglet antibiotic-free feed：Ideal amino acid ratio Lys under the conditions of disease-resistant:(Met+Cys):Thr:Trp:
Arg:Val=100:65:77:22:108:90.
The complex enzyme is constituted：Cellulase 1, protease 2, AMS 2；
The B B-complex is constituted：6 parts of vitamin D, 0.8 part of biotin, 1 part of folic acid；
The composite trace element composition is 1 part of yeast selenium, 2 parts of zinc oxide.
The yucca extract is provided by the industrial Science and Technology Ltd. of occasion of Beijing China.
The piglet antibiotic-free feed in：After operational phase was for wean two weeks --- the piglet of 15kg.
The probiotics is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and preservation is compiled
Number be CGMCC No.12789；
The saccharomycete is prepared by this area conventional method culture.
Embodiment 3
A kind of piglet antibiotic-free feed, is prepared from the following raw materials in parts by weight：
60 parts of corn, 2 parts of steam fish meal, 10 parts of dregs of beans, 5 parts of expanded soybean, 5 parts of flour, whey powder 6, coconut oil 0.5,
Soybean oil 0.5, antibacterial peptide 0.03, probiotics 3,0.012 part of yucca extract, 0.3 part of stone flour, 0.5 part of salt, calcium monohydrogen phosphate
1.2 parts, 0.8 part of amino acid, 0.03 part of complex enzyme, 0.4 part of B B-complex, 0.6 part of composite trace element；
The piglet antibiotic-free feed：Net energy 2450Kcal/kg, protein content 18%.
The piglet antibiotic-free feed：Ideal amino acid ratio Lys under the conditions of disease-resistant:(Met+Cys):Thr:Trp:
Arg:Val=100:65:77:22:108:90.
The B B-complex is constituted：Calciferol .5 parts, 1.2 parts of biotin, 2 parts of folic acid；
The composite trace element is constituted：2 parts of yeast selenium, 1 part of zinc oxide.
The yucca extract is provided by the industrial Science and Technology Ltd. of occasion of Beijing China.
The piglet antibiotic-free feed in：After operational phase was for wean two weeks --- the piglet of 15kg.
The probiotics is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and preservation is compiled
Number be CGMCC No.12789；
Embodiment 4
Test example：The piglet antibiotic-free feed prepared using embodiment 1-3 does following checking with commercially available similar feed
1 test material
1.1 test periods
In September, 2016-November completes.
1.2 test sites
Animal experiment is planted pig farm and is implemented on Liaoning Province Lingyuan He Fengzhong pig farms and Fengcheng City of Liaoning Province respectively.
1.3 experiment daily rations
Experiment sets totally 4 treatment of control group and test group, each 4 repetition for the treatment of.Test group Diet Formula is the present invention,
Control group is commercially available common compound feed for piglets.
2 results and analysis
2.1 Lingyuan He Fengzhong pig farms result of the tests
| Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Original body mass (kg) | 10.52 | 10.52 | 10.52 | 10.52 |
| Daily gain (g) | 400 | 435 | 438 | 442 |
| Average daily feed intake (g) | 647 | 690 | 685 | 695 |
| Feed-weight ratio | 1.62 | 1.59 | 1.56 | 1.57 |
| Diarrhea rate (%) | 1.75 | 1.59 | 1.50 | 1.55 |
Result shows：Test group daily gain improves 9.58%, and average daily feed intake improves 6.65%, diarrhea rate reduction
11.62%.
Plant pig farm result of the test in 2.2 Fengcheng City
| Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Original body mass (kg) | 10.81 | 10.81 | 10.81 | 10.81 |
| Daily gain (g) | 405 | 430 | 433 | 428 |
| Average daily feed intake (g) | 650 | 685 | 690 | 685 |
| Feed-weight ratio | 1.60 | 1.59 | 1.59 | 1.60 |
| Diarrhea rate (%) | 1.95 | 1.78 | 1.75 | 1.76 |
Result shows：Test group daily gain improves 6.26%, and average daily feed intake improves 5.64%, diarrhea rate reduction
9.57%.
Claims (8)
1. a kind of piglet antibiotic-free feed, is prepared from the following raw materials in parts by weight：Corn 50-60 parts, steam fish meal 2-4
Part, dregs of beans 10-16 parts, expanded soybean 1-5 parts, flour 3-5 parts, whey powder 3-6, coconut oil 0.5-1, soybean oil 0.5-1, antibacterial
Peptide 0.02-0.03, probiotics 1-3,0.012 part of yucca extract, stone flour 0.3-0.7 parts, salt 0.3-0.5 parts, calcium monohydrogen phosphate
0.8-1.2 parts, amino acid 0.3-0.8 parts, complex enzyme 0.03-0.05 parts, B B-complex 0.3-0.4 parts, composite trace element
0.2-0.6 parts.
2. a kind of piglet antibiotic-free feed according to claim 1, it is characterised in that：The complex enzyme is constituted：Fiber
Plain enzyme 1, protease 2, AMS 2.
3. a kind of piglet antibiotic-free feed according to claim 1, it is characterised in that：The B B-complex is constituted：
Calciferol .5-6 parts, biotin 0.8-1.2 parts, folic acid 1-2 parts.
4. a kind of piglet antibiotic-free feed according to claim 1, it is characterised in that：The composite trace element composition
For：Yeast selenium 1-2 parts, zinc oxide 1-2 parts.
5. a kind of piglet antibiotic-free feed according to claim 1, it is characterised in that：The probiotics is saccharomyces cerevisiae
(Saccharomyces cerevisiae) tlj2016, deposit number is CGMCC No.12789.
6. a kind of piglet antibiotic-free feed according to claim 1, it is characterised in that：Preparation method comprises the following steps：
By formula, corn and dregs of beans are first mixed, size-reduced machine is crushed, grinding screen plate aperture 1.0mm, then quenched 1min,
0.272-0.275Mpa steam pressures with 80 degree under the conditions of product is cured, then raw material after curing is mixed with other raw materials
Close, pelletized under the conditions of 65 degree.
7. a kind of piglet antibiotic-free feed according to claim 1, it is characterised in that：By the raw material system of following parts by weight
Into：
55 parts of corn, 3 parts of steam fish meal, 13 parts of dregs of beans, 3 parts of expanded soybean, 4 parts of flour, whey powder 4, coconut oil 0.5, soybean
Oil 1, antibacterial peptide 0.02,2 parts of probiotics, 0.012 part of yucca extract, 0.5 part of stone flour, 0.4 part of salt, 1.0 parts of calcium monohydrogen phosphate,
0.6 part of amino acid, 0.04 part of complex enzyme, 0.35 part of B B-complex, 0.4 part of composite trace element；
The piglet antibiotic-free feed：Net energy 2450Kcal/kg, protein content 18%；
The piglet antibiotic-free feed：Ideal amino acid ratio Lys under the conditions of disease-resistant:(Met+Cys):Thr:Trp:Arg:
Val=100:65:77:22:108:90；
The complex enzyme is constituted：Cellulase 1, protease 2, AMS 2；
The B B-complex is constituted：4 parts of vitamin D, 1 part of biotin, 1 part of folic acid；
The composite trace element is constituted：2 parts of yeast selenium, 1 part of zinc oxide；
The yucca extract is provided by the industrial Science and Technology Ltd. of occasion of Beijing China；
The piglet antibiotic-free feed in：After operational phase was for wean two weeks --- the piglet of 15kg；
The probiotics is saccharomycete；
The saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and deposit number is
CGMCC No.12789；
The saccharomycete cultivates preparation by the following method：
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm in the 250mL shaking flasks equipped with 30mL Shake flask mediums, 30 DEG C of cultures is accessed
30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Seed liquor is pressed into 10% mass percent inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, throughput
6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, and disposable addition is eventually
Concentration is the Cys of 25mmol/L, and total fermentation time is 50h；
Fermentation is finished, and pH is to 6.5 for regulation, is spray-dried to obtain yeast bacteria powder；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0；
The preparation method of the piglet antibiotic-free feed, comprises the following steps：By formula, corn and dregs of beans are first mixed, passed through
Pulverizer is crushed, grinding screen plate aperture 1.0mm, then quenched 1min, in 0.272-0.275Mpa steam pressures and 80 degree of conditions
Under product is cured, then will curing after raw material mix with other raw materials, under the conditions of 65 degree granulation.
8. a kind of preparation method of piglet antibiotic-free feed, comprises the following steps：By formula, corn and dregs of beans are first mixed,
Size-reduced machine is crushed, grinding screen plate aperture 1.0mm, then quenched 1min, in 0.272-0.275Mpa steam pressures and 80 degree of bars
Product is cured under part, then raw material after curing is mixed with other raw materials, pelletized under the conditions of 65 degree.
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| CN107821818A (en) * | 2017-11-23 | 2018-03-23 | 安徽金牧饲料有限公司 | A kind of environmentally friendly piglet feed and preparation method thereof |
| CN108094324A (en) * | 2018-01-17 | 2018-06-01 | 浙江金华乌啦乌两头乌产业发展有限公司 | The green ecological cultural method of both ends crow pig |
| CN110150481A (en) * | 2018-01-17 | 2019-08-23 | 浙江大飞龙动物保健品股份有限公司 | Porkling special health feed |
| CN108159395A (en) * | 2018-01-18 | 2018-06-15 | 长沙绿叶生物科技有限公司 | A kind of preparation for preventing and treating milk cow limb hoof disease and preparation method thereof |
| CN109362948A (en) * | 2018-10-23 | 2019-02-22 | 南宁市黄陈生猪养殖场 | A method of Se-enriched feedstuff additive is prepared using Moringa waste wood |
| CN109548963A (en) * | 2019-01-02 | 2019-04-02 | 海南舜钦生物科技有限公司 | A kind of mixed type the feed for piglet additive and preparation method thereof |
| CN110122692A (en) * | 2019-05-28 | 2019-08-16 | 常熟市金牧药业有限公司 | Swine rearing compound premix |
| CN110651909A (en) * | 2019-11-15 | 2020-01-07 | 吉林农业大学 | Plant source milk powder substitute for piglets and application thereof |
| CN110742198A (en) * | 2019-11-21 | 2020-02-04 | 中国科学院烟台海岸带研究所 | Antibiotic reduction preparation for breeding industry and preparation method and application thereof |
| CN112006166A (en) * | 2020-07-22 | 2020-12-01 | 湖南师范大学 | Serine feed additive for improving survival rate and growth speed of piglets and application thereof |
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