CN106721070A

CN106721070A - A kind of mixed type piglet feed and preparation method thereof

Info

Publication number: CN106721070A
Application number: CN201611062388.3A
Authority: CN
Inventors: 岳隆耀; 王丹; 刘金; 胡国清; 袁亚莉; 王振勇; 邵彩梅
Original assignee: Liaoning Hefeng Animal Husbandry Co ltd
Current assignee: Liaoning Hefeng Animal Husbandry Co ltd
Priority date: 2016-11-24
Filing date: 2016-11-24
Publication date: 2017-05-31

Abstract

The present invention relates to a kind of mixed type piglet feed and the preparation method of the feed, belong to feed processing technology field.The mixed type piglet feed, is mixed by powder with particulate material grade ratio, and powder primary raw material is：Popcorn, puffed rice, swelling soya dreg, expanded soybean, soybean oil, super steam fish meal, flour, fermented bean dregs, egg powder, milk powder, lactose etc..Particulate material primary raw material is：Corn, rice, dregs of beans, expanded soybean, super steam fish meal, flour, wheat bran, beet pulp, fermented bean dregs, egg powder, milk powder, lactose etc..Probiotic is with the addition of in powder and particulate material, particularly with the addition of deposit number is the rich S. cervisiae tlj2016 for producing glutathione of CGMCCNo.12789, can improve the physical function of sucking pig.Particulate material mixes with powder, greatly reduces the stress reaction after wean, sucking pig is smoothly transitted from nursing period to weaning period.Feed supplement hardly possible is solved, wean difficult problem.

Description

A kind of mixed type piglet feed and preparation method thereof

Technical field

The invention belongs to feed processing technology field, more particularly to a kind of mixed type piglet feed, and it is related to the feed Preparation method.

Background technology

Weanling pig feed becomes solid feed from liquid milk, and living environment is survived by by parent to independent, if raised It is improper to support, and will cause piglet dysphoria, lose the appetite, stagnations of growing, forms cad pig, even ill and death.Most Suitable Weaning Age is 28-35 days, should do sth. in advance to start feeding training, piglet is searched for food well feed, is conducive to piglet Grow.

The principal element for influenceing Production Performance of Weaning Pigs is because source of nutrition is become solid by breast milk completely after wean Body feedstuff, the butterfat that can be digested and assimilated completely, albumen in breast milk are replaced by cereal starch, vegetable protein, and in feed Also contain a certain amount of crude fibre.Piglet causes grice diarrhoea to the inadaptable of feed, and grice diarrhoea is dead weanling pig One of the main reasons, the major measure that most of plants solve wean sucking pigs diarrhoea at present is to use antibiotic, but anti- The use of raw element can suppress the growth and breeding of beneficial bacterium, influence the development of enteron aisle, and can produce drug resistance, medicine in body The problems such as residual and unabsorbed antibiotic are to the pollution of environment.Abuse of antibiotics can also indirect hazard human health and safety. Therefore the piglet feed of antibiotic-free is developed, the nutritional need for meeting weanling pig is particularly important to improving pig farm economic benefit. The feed shape of weanling pig has many kinds, including mash feed, pellet, expanded pellet diet, biological feedstuff and atherosclerotic feed Etc..China's feed only has powder when at the early-stage；After granulating process introduces China, particularly the mid-80 Pellet has obtained swift and violent development in China；Recent years, expanded pellet diet have developed rapidly；Also there is atherosclerotic material at present.Not jljl The drawbacks of feed of reason form has respective.Research to piglet feed is relatively more, such as it is following these：

Publication number:The Chinese invention of 102356824A《Sucking pig nutrient fodder and the mixing of the sucking pig containing the nutrient fodder are raised Material》Disclose a kind of sucking pig nutrient fodder and the sucking pig mixed fodder containing the nutrient fodder, each component in sucking pig nutrient fodder Weight content it is as follows：25 parts of fermented bean dregs, expanded soybean 10-20 parts, lactose 10-20 parts, rice protein 8-10 parts, vegetable oil 6-8 parts, calcium monohydrogen phosphate 5-6 parts, stone flour 5-6 parts, fish meal 4-6 parts, 1B 2-3 parts, yeast nucleic acid 2-3 parts, salt 1-2 Part, citric acid 1-2 parts, zinc oxide 0.5-1.0 parts, 15% aureomycin 0.5-1.0 parts, threonine 0.5-1.0 parts, DL- METHIONINE 0.4-0.6 parts, 0.06 part of Kitasamycin, Organic Iron 0.1-0.2 parts, flavouring agent 0.2-0.4 parts, 0.25 part of vitamin, micro unit 1.0 parts and choline 0.5-1.0 parts of element.The feed can promote piglet to grow rich in yeast nucleic acid, and palatability and attractant are good, can Increase the feed intake of weanling pig, the enteron aisle for improving its average daily gain and adapting to weanling pig is digested and assimilated, significantly reduced The diarrhea rate of weanling pig.From the table of comparisons as can be seen that feeding 31 ages in days to 60 age in days piglets using mixed fodder of the invention, Average daily feed intake improves 6.15% up to 694.25 gram/days than feeding basal diet 532；Average daily gain is up to 435.25 Gram/days, the piglet than feeding basal diet averagely improves 11.32%；The age in days Duodenal villi of piglet 60 highly reaches 532 μm, the piglet than feeding basal diet averagely improves 26.67%, and diarrhoea phenomenon does not occur.P values in the table of comparisons I.e. probability, reflects the possibility size that a certain event occurs, and P values, P are obtained according to significance test method<0.05 is notable, P< 0.01 is highly significant.

Publication number:The Chinese invention of 105918670A《A kind of piglet feed》Disclose a kind of piglet feed, the feed Component including following parts by weight：400-500 parts of the peony seeds dregs of rice；Whey powder 50-60 parts；60-70 parts of goose rice noodle roll；Plasma protein Powder 65-80 parts；Oil-bound distemper 30-40 parts；Glucose 40-45 parts；4 parts of 1B；DL- METHIONINE 0.5-1 parts；Zinc oxide 0.5- 1.2 parts；Colistine sulfate 6-8 parts；Calcium dihydrogen phosphate 1-3 parts；Yeast chromium 5-8 parts, the present invention to the selection of the species of material and Content selection be according to the digestive physiology of piglet, grow, weaning stage the characteristics of carry out so that each nutrients in feed Matter collocation is reasonable, is not only adapted to digesting and assimilating for wean sucking pig, makes the growth rate of wean sucking pig while significantly improving, also The immunity of wean sucking pig is improve, the food security of pork is increased, while after the effect of vinasse can cause sucking pig baking Fertilizer and it is oiliness.

Publication number:The Chinese invention of 105685402A《A kind of piglet feed and preparation method thereof》A kind of sucking pig is disclosed to raise Material and preparation method thereof, including following weight portion raw material：Dregs of beans 120-180 parts, wheat bran 40-60 parts, corn flour 30-50 parts, Peanut shell 30-50 parts, fish meal 10-20 parts, bone meal 3-8 parts, 99% methionine 0.2-0.8 parts, 98% threonine 0.4-0.8 parts, 98% lysine 1-4 parts, phytase 0.2-0.5 parts, B B-complex 0.1-0.8 parts, Choline Chloride 8-18 parts, ferrous sulfate 10-15 parts, copper sulphate 5-10 parts, manganese sulfate 3-8 parts, zinc sulfate 3-8 parts, KI 0.2-0.8 parts, sodium selenite 0.05- 0.16 part, antioxidant 8-18 parts, mould inhibitor 18-25 parts and flavor enhancement；Piglet feed of the invention is easy to digest, easy absorption, its Porkling fast growth, survival rate is high.

Publication number:The Chinese invention of 105614123A《A kind of piglet feed and preparation method thereof》A kind of piglet feed, bag Include following each component count by weight percentage：Corn flour 45-55%；Millet powder 15-25%；Soybean oil 5- after emulsification 8%；Whey powder 3-8%；Fish meal 5-10%；SDPP 4-6%；Skimmed milk power 2-4%；Glucose 1-5%；Premix 5- 9%.Its preparation method, comprises the following steps：First, ultrasonic emulsification 1-2h at oleic acid, 30-35 DEG C is added in soybean oil, is obtained final product Soybean oil after emulsification；2nd, by the soybean oil after emulsification, corn flour, millet powder, whey powder, fish meal, SDPP, degreasing Milk powder, glucose, premix mix in proportion, by granulation, obtain final product described piglet feed.

Publication number:The Chinese invention of 105558376A《A kind of wean piglet feed of antibiotic-free and preparation method thereof》It is public A kind of wean piglet feed of antibiotic-free is opened, the feed is made up of the raw material including following parts by weight：Expanded soybean 10-18 parts, dehulled soybean meal 12-20 parts, corn 40-60 parts, 5-10 parts of high protein whey powder, wheat bran 2-5 parts, rice 6-10 parts, Soya-bean oil 2-5 parts, glucose 2-3 parts, calcium lactate 0.8-1 parts, glutamine 2-5 parts, salt 0.1-0.5 parts, acidulant 0.8-1.5 Part, probiotic 0.2-0.5 parts, complex enzyme formulation 0.2-0.5 parts, lysozyme 0.02-0.05 parts, montmorillonite 0.1-0.3 parts, it is micro 0.1-0.5 parts of element premix, vitamin premix 0.03-0.05 parts, the wean piglet feed, its amino acid balance, energy Amount is higher, and rich in phagostimulant, with the discharge reduced to nitrogen in environment and phosphorus, good palatability, digestibility is high, improves colony balance With the fine hair development of promotion enteron aisle, without antibiotic, the advantage of enhancing anti diar rhea ability and immunity.

The feed for being wean sucking pig of piglet feed concern disclosed in prior art, may not in view of nursing period sucking pig Because sows farrowing is more, piglet ontogeny is unbalanced, rob the reasons such as food sucking pig can may be caused malnutritive, survival rate is low to ask Topic, in addition, the single Feed forms such as existing particulate material, powder, in the presence of it is certain the drawbacks of, particulate material can reduce enzyme activity Property, reduce vitamin potency.Powder is easily classified, and palatability is poor, because feedstuff unavoidably has some piglets not like Smell, can sufficiently be come out in powder.Therefore for sucking pig, there is feed supplement in the feed of existing single kind Difficulty, wean difficult problem.

The content of the invention

In order to solve the above problems, we provide a kind of feed of powder mixing, solve feed supplement hardly possible, and wean difficult problem, In addition the present invention with the sucking pig of 7 ages in days-wean two weeks afters be research object, the sucking pig feeding mixed with particulate material grade ratio using powder Material, powder stresses food calling, and particulate material stresses insoluble fiber and improves feed intake protection intestinal health, makes up two material types not Foot, is searched for food after taking into account feed supplement and wean, and two stages are solved the problems, such as with a kind of feed.

The technical solution adopted by the present invention is：A kind of mixed type piglet feed is mixed by powder with particulate material grade ratio, The powder is made up of the raw material including following parts by weight：

Popcorn 10-30 parts, puffed rice 10-20 parts, swelling soya dreg 3-5 parts, expanded soybean 5-12 parts, soybean oil 0.5-2 parts, super steam fish meal 3-5 parts, flour 6-10 parts, fermented bean dregs 2-3 parts, egg powder 2-5 parts, milk powder 5-8 parts, lactose 5-10 parts, glucose 2-3 parts, white sugar 1-3 parts, acidulant 3-5 parts, calcium monohydrogen phosphate 0.4-0.5 parts, amino acid 0.2-0.5 parts, height Warm proteinase-10 .01-0.03 parts, B B-complex 0.04-0.06 parts, composite trace element 0.2-0.6 parts, probiotic 0.1-0.3 Part；

The particulate material is made up of the raw material including following parts by weight：

Corn 10-30, rice 10-20, dregs of beans 3-5, expanded soybean 5-12, super steam fish meal 3-5, flour 6-10, wheat Bran 1-3, beet pulp 1-3, fermented bean dregs 5-8, egg powder 2-5, milk powder 2-5, lactose 3-7, glucose 2-3, white sugar 1-3, acidifying Agent 3-5, calcium monohydrogen phosphate 0.4-0.5, amino acid 0.2-0.5, thermoase 0.01-0.03, B B-complex 0.04-0.06, Composite trace element 0.2-0.6, probiotic 0.1-0.3 parts；

The probiotic is made up of Lactobacillus plantarum, bacillus licheniformis, saccharomycete, the Lactobacillus plantarum, lichens bud Spore bacillus, the living bacteria count of saccharomycete are respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, ferment Female bacterium >=10,000,000,000/g；

Preferably, the saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, Deposit number is CGMCC No.12789；

Preferably, the saccharomycete cultivates preparation by the following method：

(1) Shaking culture

The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed Culture 30h obtains seed liquor；

Shake flask medium (g/L)：(NH₄)₂SO₄6th, glucose 35, K₂HPO₄·3H₂O 3、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1, pH6.0；

(2) 5L fermentation tank cultures

Seed liquor is pressed into 10% inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, throughput 6L/ Min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably add final concentration It is the Cys of 25mmol/L, total fermentation time is 50h；

Fermentation is finished, and pH is to 6.5 for regulation, is spray-dried to obtain yeast bacteria powder；

Fermentation medium (g/L)：(NH₄)₂SO₄10th, glucose 100, K₂HPO₄·3H₂O 8、KH₂PO₄0.5th, dusty yeast 11、MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1,pH6.0；

The composite trace element contains the component of following concentration：Ferrous fumarate 50000mg/kg, cupric sulfate pentahydrate 100000mg/kg, manganese sulfate monohydrate 52000mg/kg, monohydrate zinc sulphate 48000mg/kg, yeast selenium 50000mg/kg, calcium iodate 300mg/kg, chromium picolinate 50000mg/kg；

The B B-complex contains the component of following concentration：Dehydroretinol 0000KIU/kg, vitamin D₃8000KIU/ Kg, vitamin e1 00000mg/kg, vitamin B₁₂30mg/kg, vitamin B₆6000mg/kg, vitamin B₁2000mg/kg, dimension Raw element B₂10000mg/kg, nicotinic acid 20000mg/kg, calcium pantothenate 10000mg/kg, folic acid 500mg/kg, biotin 200mg/kg.

Preferably, the also zinc containing 200ppm in the powder, the zinc comes from zinc sulfate；

Preferably, the also zinc containing 2250ppm in the particulate material, the zinc comes from zinc oxide；

Present invention simultaneously provides a kind of preparation method of mixed type piglet feed, comprise the following steps：By each raw material 15HZ Ultramicro grinding is well mixed to after 260um, obtains powder；55 DEG C of low temperature are pelletized to obtain particulate material；By powder and particulate material according to 1：1 Ratio mix to obtain mixed type piglet feed.

Beneficial effect：

1st, powder stresses food calling

Powder is easy to feeding, is added water in feed when mash feed is fed and is stirred, and is so both avoided that powder Dirt produces the feed intake for stimulating and improving pig again.Powder is cheap compared with particulate material, and nutritional ingredient keeps complete, young to wean The stimulation of swine alimentary canal is smaller.5-8 parts of milk powder is added in powder, 5-10 parts of lactose makes mouthfeel closer to sow milk, energy Enough attract sucking pig feeding, reduce stress reaction.The popcorn and 3-5 parts of swelling soya dreg of 10-30 parts of addition, are conducive to wean young Pig digests and assimilates.

2nd, particulate material stresses insoluble fiber, it is possible to increase sucking pig feed intake, protects intestinal health, child care transition

Wean be piglet face in life it is powerful stress a stage, frequently result in feed intake rapid drawdown, it is slow-growing very To stopping growing, there is significant change in the 26S Proteasome Structure and Function of intestines and stomach.Piglet gastric acid output is not enough, indigested nutrients Matter is assembled in small intestine and protein ferments in enteron aisle rear end, is all the key factor for causing pathogenic entero becteria largely to breed.By The digestibility of piglet can be influenceed in cellulose, feed intake is reduced, so the industry can often control cellulose in piglet diet Content.However, increasing evidence shows that SNSP can promote carbohydrate fermentation in daily ration, so as to strengthen The vigor of enteron aisle rear end microorganism.Wheat bran contains insoluble SNSP very high, and megasse contains very high soluble non- Starch-polysaccharides.Soluble Non Starch Polysaccharides are generally possible to fully ferment, and insoluble fibers may then increase the water holding that food wastes Power and slowly fermented by microorganism in enteron aisle rear end, the two is cooperated in certain proportion, both do not interfere with sucking pig Digest and assimilate, the vigor of enteron aisle rear end microorganism can be well strengthened again.

3rd, solve that two kinds of feeds, two feeding periods of solution should be used, two stages are solved with a kind of feed Problem

In pelleted ration, the powder of some fat contents high is mixed into, to avoid compound from being transported by the long period With the separation of generation composition after treatment.Do so, is in order that these feeds are initially sprinkling upon ground after being suitable for wean in 3-5 days On the mat in face or be sprinkling upon circle feed trough in eaten to piglet.In our experiment, feed is sprinkling upon on mat for three days on end. Demonstrate this advantage of this compound on evidence quickly, that is, these feeds can always present on mat without Trickle down.Powder in compound is nutritious with particulate material and balanced, and powder part touches the oral cavity of piglet first, and Stick in the oral cavity of piglet, the nutrition in enabling piglet to obtain powder.Particle also very convenient sucking pig feeding, it contains abundant Energy, proteins,vitamins,and minerals, can make piglet keep wean after high-speed rapid growth.Still further aspect, even if young Pig is eaten particulate material part or only eats powder part, due to two kinds of nutritional needs for expecting meet sucking pig, even if sucking pig is inclined Food, the whole nutrients needed for also obtaining it.

4th, powder and particulate material respectively have feature again, meet the different demands of sucking pig, enable sucking pig from nursing period to wean Phase smoothly transits, and farthest reduces the stress reaction after wean.

Powder simulates sow milk, milk powder type；Granulated milk powder content is low, and fermented bean dregs are high, stimulates intestinal growth, enteron aisle to supply Can, it is easy to be connected with child care material.Powder 15HZ ultramicro grindings, raw material curing, just melt in the mouth；Particulate material is low temperature granulation, dimension life Element, enzyme preparation etc. keep high activity, and pretreatment of raw material is cured, grease high, and well, molten water is fast, and hardness is low for shortcakeization.

Present invention addition thermoase, the absorption beneficial to sucking pig to nutriment can reduce the row of animal nitrogen and phosphorus Put, reduce environmental pollution；Mixed fodder of the invention can adjust electrolyte balance, mainly optimize the water metabolism in sucking pig body Balance, prevents pig to be dehydrated the death for causing pig；Soybean uses high temperature puffing, mainly reduce soybean contain various anti-nutrition because Son, such as trypsin ihhibitor, phytolectin, flatulence factor, antigen protein, improve soybean nutritional digestibility and Palatability, improves animal growth；Pelletized using low temperature, increasing the curing degree of starch, while improving feed palatability, The heat loss of vitamin and enzyme, and protein denaturation loss and the loss of functional additive can be reduced；Additionally, addition Probiotic can suppress the propagation of harmful bacteria, promote the propagation of beneficial bacterium, improve the development of sucking pig intestinal villi, reduce feed abdomen Rate is rushed down, animal immunizing power is improved；The preservation of bacteria strain tlj2016 of high yield glutathione is particularly added, glutathione is used as internal Important antioxidant and free radical scavenger, is such as combined with free radical, heavy metal, so as to the poisonous substance being harmful in body is turned Harmless material is turned to, external, the physical function to improving sucking pig is excreted, it is very helpful；The present invention also addition acidulant, phosphorus Sour hydrogen calcium, can reduce neutralization of the feed to hydrochloric acid in gastric juice, improve the release of stomach cardia, improve feed digestibility, reduce sucking pig diarrhoea Rate；Additionally, mainly with the addition of ferrous fumarate in trace element, the use of Organic Iron is emphasized, improve the absorptivity of iron, prevent breast Blows, the fur for improving animal；The experiment proved that, herein described Feed Energy significantly improves the feed intake of wean sucking pig, increase Plus daily gain, while reducing diarrhea rate.

Specific embodiment

The Wine brewing yeast strain is specially saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, the bacterium Strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number on July 15th, 2016 It is CGMCCNo.12789, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, Postcode 100101.

The most suitable growth pH of the saccharomyces cerevisiae tlj2016 is 6.0-6.5, and optimum growth temperature is 28-35 DEG C；

The saccharomyces cerevisiae tlj2016 is isolated saccharomyces cerevisiae starting strain from the orchard of one plant of Ningxia through following Step is obtained：

The original strain that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → hypertonic plate screening → nitrosoguanidine (NTG) secondary screening (producing glutathione GSH abilities) → passage stabilization is screened → fermented to mutagenesis screening → hypertonic flat board primary dcreening operation → shaking flask Property experiment.

By the bacterial strain tlj2016 obtained after mutagenesis, its glucose tolerance and Cys tolerance are equal It is improved, cell density, another aspect Cys tolerance energy on the one hand can be improved under high concentration glucose culture The raising of power is beneficial to GSH and largely synthesizes in intracellular, so as to improve the ability that bacterial strain mass produces GSH.

Saccharomyces cerevisiae of the present invention reaches 300g/L to the tolerance of glucose, beneficial to it in high concentration grape GSH is produced under the conditions of sugar；3308mg/L is reached in 5L fermentation cylinder for fermentation production GSH final concentrations；Tolerate the energy of Cys Power is far above starting strain, slow growth is remained under the effect of 5mmol/L Cys, in 40mmol/LL- cysteines Remain to keep GSH largely to synthesize under effect；Salt resistance ability reaches 18%, is conducive to extending its application field.

1.DES mutagenic and breedings

1) ring of starting strain 1 one on test tube slant is taken on super-clean bench, is accessed equipped with 50mL malt extract mediums In 250mL triangular flasks, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.

2) 5mL bacterium solutions are taken, 5000rpm centrifugation 10min collects thallines, with brine 2 times.

3) 10 are diluted to pH7.0 phosphate buffers⁷Individual/mL bacteria suspensions.

4) kaliumphosphate buffer of 32mL pH7.0,8mL bacteria suspensions, 0.4mL DES are taken and is being placed in advance in the 150mL of rotor It is sufficiently mixed in triangular flask, makes DES ultimate densities be 1% (v/v).

5) the 150rpm reactions 30min in 30 DEG C of shaking tables, takes 1mL mixed liquors, adds 0.5mL 25%Na₂S₂O₃In solution Only react.

6) dilution spread is in the malt extract medium plate containing 150g/L KCl, the picking bacterium after 30 DEG C of cultures 2-3 days The bacterial strain 1 of the maximum that falls.

2. nitrosoguanidine mutagenesis

1) ring of saccharomyces cerevisiae bacteria strain 1 one on test tube slant is taken on super-clean bench, is accessed and 50mL brewer's wort cultures is housed In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.

2) 5mL bacterium solutions 5000rpm centrifugation 10min collects thallines are taken, with brine 2 times.

3) 10 are diluted to pH6.0 phosphate buffers⁷Individual/mL bacteria suspensions.

4) 10mL bacteria suspensions are taken to be transferred in 100mL triangular flasks, the NTG of 10mg is added, final concentration of 10mg/mL is configured to NTG solution, and add 4-5 drop acetone, be beneficial to NTG dissolvings.

5) the 200rpm oscillating reactions 30min at 30 DEG C, 5000rpm are centrifuged 10min collects thallines, use SPSS Wash for several times, stopped reaction.

6) appropriate dilution spread, takes the bacterium solution 0.2mL of last dilution factor, coats the brewer's wort culture containing 200g/L KCl In base plate.Picking colony 20 after being cultivated 2-3 days at 30 DEG C.

3. shaking flask primary dcreening operation

1) take above-mentioned each ring of 20 S. cervisiaes respectively on super-clean bench, be respectively connected to equipped with 50mL brewer's wort cultures In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 12h or so, thalline is in mid log phase.

2) 5mL bacterium solutions are taken, is accessed equipped with the 250mL in the hypertonic malt extract mediums of 50mL (concentration of glucose is 300g/L) In triangular flask, 200rpm, 30 DEG C are cultivated 3-4 days, daily detection concentration of glucose and concentration of alcohol change.After fermentation ends, than Compared with 20 plants of glucose and ethanol wear rate of strain, final remaining sugar concentration and concentration of alcohol, glucose to the conversion ratio of ethanol And heteroacid content.

3) glucose consumption rate is fast, final remaining sugar concentration is low and concentration of alcohol is high 5 plants of bacterium are named as Y-1, Y- for selection 2, Y-3, Y-4, Y-5.

4. ferment secondary screening

By 5 plants of bacterium Y-1, Y-2, Y-3, Y-4, Y-5 and starting strain of gained in step 3, respectively according to 10% inoculation Amount, the 150rpm in the 250mL shaking flasks equipped with 30mL fluid nutrient mediums, 30 DEG C of culture 30h, takes zymotic fluid and determines GSH concentration；

Fluid nutrient medium (g/L)：(NH₄)₂SO₄6th, glucose 35, K₂HPO₄·3H₂O 3、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1；

GSH assay methods：Fresh yeast, at 30 DEG C, 40% Ethanol Treatment 3h, in centrifuging and taking are obtained after zymotic fluid centrifuge washing Clear liquid is cooked GSH determination samples；

GSH measure is carried out using alloxan method：Its principle is that-the SH on GSH reacts with alloxan, the material of generation There is absworption peak at 305nm, and it is linear with glutathione concentrations, therefore can be quantitative determined with ultraviolet specrophotometer GSH contents.

Table 1：GSH testing results

Bacterial strain	Starting strain	Y-1	Y-2	Y-3	Y-4	Y-5
							GSH concentration (mg/L)	127.9	195.7	172.3	263.2	216.5	185.2

As seen from the results in Table 1, bacterial strain Y-3 has highest GSH fermentabilities, it is thus determined that Y-3 is final production bacterium Strain, and it is named as tlj2016.

5. genetic stability experiment

Continuous ten passages on inclined-plane by tlj2016 bacterium, and detect the hair after passage every time with the method for shaking flask secondary screening Ferment situation.Experiment finds that continuous ten passages on inclined-plane, the strain proterties does not have significant change, and property indices are all just Often, illustrate that the genetic stability of the strain is stronger.

Fermented under the conditions of saccharomyces cerevisiae tlj2016 sugar high and produce GSH capacity experimentals

(1) Shaking culture

(2) 5L fermentation tank cultures

After fermentation ends, the content for determining GSH in zymotic fluid is 3308mg/L.

Saccharomyces cerevisiae tlj2016L- cysteines tolerance is tested

By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to 150rpm in shaking flask, 30 DEG C are cultivated, when culture is to 12h, to the Guang ammonia of L- half that different final concentrations are added in shaking flask Acid, is further cultured for 10h, determines dry cell weight, as a result table 2,3；

Shake flask medium (g/L)：(NH₄)₂SO₄6th, glucose 20, K₂HPO₄·3H₂O 3、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1, pH6.0；

Table 2：Starting strain Cys tolerance

Cys concentration mmol/L	0	5	10	15	20	40
							Starting strain dry weight g/L	22.6	15.7	10.2	4.3	2.2	0.8
GSH concentration (mg/L)	35.6	46.7	43.2	40.7	37.9	25.3

The tlj2016L- cysteine tolerances of table 3

Cys concentration mmol/L	0	5	10	15	20	40
							Tlj2016 dry weights g/L	25.7	28.5	23.6	21.2	20.6	18.7
GSH concentration (mg/L)	73.2	98.3	113.5	121.7	127.5	135.8

From the results shown in Table 2, for starting strain, Cys are added in culture medium, cell stops growing, And start self-dissolving, cause GSH growth rates to be reduced with the rising of Cys concentration；And low concentration Cys Under, tlj2016 still is able to slow growth, with the raising of Cys concentration, under the dry cell weight of tlj2016 bacterial strains is slow Drop, and GSH concentration sustainable growths, this result is beneficial in GSH production processes by adding the Guang ammonia of precusor amino acids-L- half Acid promotes the production of GSH.

Saccharomyces cerevisiae tlj2016 salt resistance abilities are tested

Take tlj2016 bacterium solutions 1mL inoculation strain in containing different NaCl concentrations (concentration gradients be 0%, 2%, 5%, 10%th, 15%, 10mL YPD fluid nutrient mediums (pH=6.5) 18%), 24h is cultivated at being placed in 30 DEG C respectively, each treatment 3 Individual repetition.Respectively take 1ml samples bacterium solution to be mixed in 9ml physiological saline, prepare dilution factor solution, take 0.1ml dilutions solid in YPD It is coated with body flat board, culture 36 hours (each dilution factor do 3 parallel) record is inverted in 30 DEG C of biochemical cultivation cases and calculates flat Bacterium number number on plate.The results are shown in Table 4, it is known that the resistance to salinity of the bacterium is 18%, illustrates that tlj2016 not only can be in conventional ring Survived in border, still there is vigor under high salt conditions, can be applied to consume sugar in the high salt food processing process such as soy sauce, curing food Produce glutathione.

The salt resistance ability of table 4 detection (× 10⁷cfu/ml)

Embodiment 1

A kind of mixed type piglet feed is mixed by powder with particulate material grade ratio, and the powder is by including following weight portion Several raw materials are made：

20 parts of popcorn, 15 parts of puffed rice, 4 parts of swelling soya dreg, 8 parts of expanded soybean, 1 part of soybean oil, super steam 4 parts of fish meal, 8 parts of flour, 3 parts of fermented bean dregs, 4 parts of egg powder, 6 parts of milk powder, 8 parts of lactose, 2 parts of glucose, 2 parts of white sugar, acidifying 4 parts of agent, 0.4 part of calcium monohydrogen phosphate, 0.3 part of amino acid, 0.02 part of thermoase, 0.05 part of B B-complex, compound micro unit Plain 0.4 part, 0.2 part of probiotic；

Corn 20, rice 15, dregs of beans 4, expanded soybean 8, super steam fish meal 4, flour 8, wheat bran 2, beet pulp 2, fermentation Dregs of beans 6, egg powder 4, milk powder 3, lactose 5, glucose 2, white sugar 2, acidulant 4, calcium monohydrogen phosphate 0.4, amino acid 0.4, high temperature egg White enzyme 0.02, B B-complex 0.05, composite trace element 0.4,0.2 part of probiotic；

The saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016；

The saccharomycete cultivates preparation by the following method：

(1) Shaking culture

(2) 5L fermentation tank cultures

The also zinc containing 200ppm in the powder, the zinc comes from zinc sulfate；

The also zinc containing 2250ppm in the particulate material, the zinc comes from zinc oxide；

A kind of preparation method of mixed type piglet feed, comprises the following steps：By each raw material 15HZ ultramicro grindings to 260um Afterwards, it is well mixed, obtains powder；55 DEG C of low temperature are pelletized to obtain particulate material；By powder and particulate material according to 1：1 ratio mixes must be mixed Type piglet feed.

Embodiment 2

10 parts of popcorn, 20 parts of puffed rice, 5 parts of swelling soya dreg, 5 parts of expanded soybean, 0.5 part of soybean oil, super steaming 5 parts of vapour fish meal, 6 parts of flour, 3 parts of fermented bean dregs, 5 parts of egg powder, 5 parts of milk powder, 5 parts of lactose, 2 parts of glucose, 3 parts of white sugar, acid It is 3 parts of agent, 0.4 part of calcium monohydrogen phosphate, 0.5 part of amino acid, 0.03 part of thermoase, 0.06 part of B B-complex, compound micro 0.6 part of element, 0.3 part of probiotic；

Corn 30, rice 20, dregs of beans 5, expanded soybean 5, super steam fish meal 5, flour 10, wheat bran 3, beet pulp 1, fermentation Dregs of beans 5, egg powder 5, milk powder 5, lactose 7, glucose 2, white sugar 1, acidulant 5, calcium monohydrogen phosphate 0.5, amino acid 0.5, high temperature egg White enzyme 0.03, B B-complex 0.06, composite trace element 0.6,0.1 part of probiotic；

The saccharomycete cultivates preparation by the following method：

(1) Shaking culture

(2) 5L fermentation tank cultures

Embodiment 3

30 parts of popcorn, 20 parts of puffed rice, 5 parts of swelling soya dreg, 12 parts of expanded soybean, 2 parts of soybean oil, super steam 5 parts of fish meal, 10 parts of flour, 3 parts of fermented bean dregs, 5 parts of egg powder, 8 parts of milk powder, 10 parts of lactose, 3 parts of glucose, 3 parts of white sugar, acid It is 5 parts of agent, -0.5 part of calcium monohydrogen phosphate, 0.5 part of amino acid, 0.03 part of thermoase, 0.06 part of B B-complex, compound micro 0.6 part of element, 0.3 part of probiotic；

Corn 30, rice 20, dregs of beans 5, expanded soybean 12, super steam fish meal 5, flour 10, wheat bran 3, beet pulp 3, hair Ferment dregs of beans 8, egg powder 5, milk powder 5, lactose 7, glucose 3, white sugar 3, acidulant 5, calcium monohydrogen phosphate 0.5, amino acid 0.5, high temperature Proteinase-10 .03, B B-complex 0.06, composite trace element 0.6,0.3 part of probiotic；

Embodiment 4

10 parts of popcorn, 10 parts of puffed rice, 3 parts of swelling soya dreg, 5 parts of expanded soybean, 0.5 part of soybean oil, super steaming 3 parts of vapour fish meal, 6 parts of flour, 2 parts of fermented bean dregs, 2 parts of egg powder, 5 parts of milk powder, 5 parts of lactose, 2 parts of glucose, 1 part of white sugar, acid It is 3 parts of agent, 0.4 part of calcium monohydrogen phosphate, 0.2 part of amino acid, 0.01 part of thermoase, 0.04 part of B B-complex, compound micro 0.2 part of element, 0.1 part of probiotic；

Corn 10, rice 10, dregs of beans 3, expanded soybean 5, super steam fish meal 3, flour 6, wheat bran 1, beet pulp 1, fermentation Dregs of beans 5, egg powder 2, milk powder 2, lactose 3, glucose 2, white sugar 1, acidulant 3, calcium monohydrogen phosphate 0.4, amino acid 0.2, high temperature egg White enzyme 0.01, B B-complex 0.04, composite trace element 0.2,0.1 part of probiotic；

The saccharomycete is prepared using this area conventional method culture；

Experimental example does following checking using mixed type piglet feed prepared by each embodiment

1 test material

1.1 test periods

Tested in May, 2015, terminate in mid-May, 2010 to raise, last 14 days.

1.2 test sites

Experiment is implemented on Lingyuan, Liaoning kind pig farm.

1.3 experimental animals

Experimental animal selects Duroc * great Bai * white three way cross sodium selenites long totally 300.

1.4 experiment daily rations

Experiment sets totally 5 treatment of control group and test group.Control group one is commercially available extraordinary particulate material, and control group two is Commercially available extraordinary powder.

2 test methods

2.1 experimental designs

This experiment uses single factor test Random Design, the Duroc × length for selecting average original body mass to be about 8.22 ± 2.72kg In vain × great Bai three way cross healthy growths pig totally 300, by the close principle similar with hereditary basis of body weight, is randomly divided into 5 Treatment, feeds the mixed type piglet feed and commercially available preferable two kinds of feeds of embodiment 1-4 respectively, and reference numeral is 1- embodiments 1st, 2- embodiments 2,3- embodiments 3, the commercially available powder of 4- embodiments 4,5-.Each 6 repetition for the treatment of, each repetition (circle) 10 Pig, male and female half and half, records feed intake at 14 days experimental periods, weighs that experiment is initial and end is heavy, and calculate daily gain, daily ingestion amount, Feed-weight ratio and diarrhea rate.

2.2 feedings and managements

Experiment swine rearing is raised on bed in confined swine housing, well-ventilated.Free choice feeding and drinking-water during experiment, by pig Conventional program carries out disinfection, expelling parasite and immune.

2.3 testing indexs

2.3.1 ration ingredient

Diet dry substance, thick protein, calcium and total phosphorus respectively refer to National Standard of the People's Republic of China GB/T 6435- 1986th, the method that GB/T 6432-1994, GB/T 6436-2002 and GB/T 6437-2002 recommend is determined.

Daily ration sample is respectively through hydrochloric acid water after performic oxidation 16h at 24h and 0 DEG C of 6mol/L hydrochloric acid hydrolysis at 110 DEG C Solution 24h determines 15 kinds of amino acid and sulfur-containing amino acid with automatic amino acid analyzer (Hitachi L-8800, Japan)；Use 4mol/L hydrogen After sodium oxide molybdena hydrolyzes 22h at 110 DEG C, tryptophan is determined using high performance liquid chromatograph (Shimadzu LC-10A, Japan).

2.3.2 growth performance

Respectively at each on-test, morning empty stomach individuality is weighed with the end of, is that unit records feed consumption to repeat (circle) Amount, calculates average daily gain, average daily gain, feed weightening ratio and diarrhea rate.

(1) average daily gain (ADG)=(initial weight-end weight)/14

(2) average daily gain (ADFI)=every per day feed intake sum/14

(3) feed weightening is than (F/G)=ADFI/ADG

(4) diarrhea rate=diarrhoea head number/(the total head number × experiment number of days of examination pig)

2.4 statistical analyses

After test data carries out preliminary treatment with Excel softwares, using the general linear model (general of SAS 8.2 Linear model, GLM) modeling statistics analysis (SAS, 2001).

3 results and analysis

Growth performance contrast table

	1	2	3	4	5	6
							Starting weight (kg)	8.22	8.23	8.21	8.22	8.22	8.22
End weight (kg)	12.04	11.60	11.51	11.45	11.08	11.13
							Average daily gain (g/d)	273	241	236	231	204	208
Average daily gain (g/d)	376	330	330	323	312	315
							Feed weightening ratio	1.38	1.37	1.40	1.40	1.53	1.51
Diarrhea rate %	1.51	2.80	2.60	2.50	4.76	3.74

Result of the test shows that feeding the experimental group growth of suckling pigs performance of each embodiment mixed fodder includes daily gain, feeding Amount all increases significantly, and average daily gain improves 19.05%, and average daily gain improves 8.40%.Feed weightening ratio, diarrhoea Rate has significant reduction.Mixed type piglet feed formula of the present invention is reasonable, and good palatability, the attractant to sucking pig is high, energy The feed intake of wean sucking pig is improved, increases daily gain.In the case where antibiotic is not added with, diarrhea rate can be reduced, greatly Reduce feed weightening ratio.

Claims

1. a kind of mixed type piglet feed, is mixed by powder with particulate material grade ratio, and the powder is by including following weight portion Several raw materials are made：

Popcorn 10-30 parts, puffed rice 10-20 parts, swelling soya dreg 3-5 parts, expanded soybean 5-12 parts, soybean oil 0.5-2 Part, super steam fish meal 3-5 parts, flour 6-10 parts, fermented bean dregs 2-3 parts, egg powder 2-5 parts, milk powder 5-8 parts, lactose 5-10 Part, glucose 2-3 parts, white sugar 1-3 parts, acidulant 3-5 parts, calcium monohydrogen phosphate 0.4-0.5 parts, amino acid 0.2-0.5 parts, high temperature egg White 0.01-0.03 parts, B B-complex 0.04-0.06 parts, composite trace element 0.2-0.6 parts, probiotic 0.1-0.3 parts of enzyme；

Corn 10-30, rice 10-20, dregs of beans 3-5, expanded soybean 5-12, super steam fish meal 3-5, flour 6-10, wheat bran 1- 3rd, beet pulp 1-3, fermented bean dregs 5-8, egg powder 2-5, milk powder 2-5, lactose 3-7, glucose 2-3, white sugar 1-3, acidulant 3- 5th, calcium monohydrogen phosphate 0.4-0.5, amino acid 0.2-0.5, thermoase 0.01-0.03, B B-complex 0.04-0.06, compound Micro- 0.2-0.6, probiotic 0.1-0.3 parts；

The probiotic is made up of Lactobacillus plantarum, bacillus licheniformis, saccharomycete, the Lactobacillus plantarum, lichens gemma bar Bacterium, the living bacteria count of saccharomycete are respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, saccharomycete >=100 hundred million/g.

2. mixed type piglet feed according to claim 1, it is characterised in that the saccharomycete is that preservation of bacteria strain is made wine ferment Female bacterium (Saccharomyces cerevisiae) tlj2016, deposit number is CGMCC No.12789.

3. mixed type piglet feed according to claim 2, it is characterised in that the saccharomycete cultivates system by the following method It is standby：

(1) Shaking culture

The ring of tlj2016 slant strains one is taken, 150rpm in the 250mL shaking flasks equipped with 30mL Shake flask mediums, 30 DEG C of cultures is accessed 30h obtains seed liquor；

(2) 5L fermentation tank cultures

Seed liquor is pressed into 10% inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, throughput 6L/min, tank Pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably add final concentration of The Cys of 25mmol/L, total fermentation time is 50h；

Fermentation is finished, and pH is to 6.5 for regulation, is spray-dried to obtain yeast bacteria powder.

4. mixed type piglet feed according to claim 3, it is characterised in that the Shake flask medium composition is, unit g/L：(NH₄)₂SO₄6th, glucose 35, K₂HPO₄·3H₂O 3、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、 FeSO₄ 0.1、MgSO₄·7H₂O 0.1, pH6.0；

The fermentation medium is constituted, unit g/L：(NH₄)₂SO₄10th, glucose 100, K₂HPO₄·3H₂O 8、 KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1,pH6.0。

5. according to any described mixed type piglet feeds of claim 1-4, it is characterised in that the composite trace element contains The component of following concentration：Ferrous fumarate 50000mg/kg, cupric sulfate pentahydrate 100000mg/kg, manganese sulfate monohydrate 52000mg/ Kg, monohydrate zinc sulphate 48000mg/kg, yeast selenium 50000mg/kg, calcium iodate 300mg/kg, chromium picolinate 50000mg/kg.

6. according to any described mixed type piglet feeds of claim 1-5, it is characterised in that the B B-complex contain with The component of lower concentration：Dehydroretinol 0000KIU/kg, vitamin D₃8000KIU/kg, vitamin e1 00000mg/kg, vitamin B₁₂30mg/kg, vitamin B₆6000mg/kg, vitamin B₁2000mg/kg, vitamin B₂10000mg/kg, nicotinic acid 20000mg/ Kg, calcium pantothenate 10000mg/kg, folic acid 500mg/kg, biotin 200mg/kg.

7. according to any described mixed type piglet feeds of claim 1-5, it is characterised in that also contain in the powder The zinc of 200ppm, the zinc comes from zinc sulfate.

8. according to any described mixed type piglet feeds of claim 1-5, it is characterised in that also contain in the particulate material The zinc of 2250ppm, the zinc comes from zinc oxide.

9. the method for preparing the claim any mixed type piglet feeds of 1-8, comprises the following steps：Each raw material 15HZ is surpassed After micro mist is broken to 260um, it is well mixed, obtains powder；55 DEG C of low temperature are pelletized to obtain particulate material；By powder and particulate material according to 1：1 Ratio mixes to obtain mixed type piglet feed.

10. the preparation method of mixed type piglet feed according to claim 9, it is characterised in that

The powder is made up of the raw material including following parts by weight：

20 parts of popcorn, 15 parts of puffed rice, 4 parts of swelling soya dreg, 8 parts of expanded soybean, 1 part of soybean oil, super steam fish meal 4 Part, 8 parts of flour, 3 parts of fermented bean dregs, 4 parts of egg powder, 6 parts of milk powder, 8 parts of lactose, 2 parts of glucose, 2 parts of white sugar, 4 parts of acidulant, 0.4 part of calcium monohydrogen phosphate, 0.3 part of amino acid, 0.02 part of thermoase, 0.05 part of B B-complex, composite trace element 0.4 Part, 0.2 part of probiotic；

Corn 20, rice 15, dregs of beans 4, expanded soybean 8, super steam fish meal 4, flour 8, wheat bran 2, beet pulp 2, fermented bean dregs 6th, egg powder 4, milk powder 3, lactose 5, glucose 2, white sugar 2, acidulant 4, calcium monohydrogen phosphate 0.4, amino acid 0.4, thermoase 0.02nd, B B-complex 0.05, composite trace element 0.4,0.2 part of probiotic.