CN106689786A - Compound feed for promoting development of muscular stomach of broiler and preparation method of compound feed - Google Patents
Compound feed for promoting development of muscular stomach of broiler and preparation method of compound feed Download PDFInfo
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- CN106689786A CN106689786A CN201611189253.3A CN201611189253A CN106689786A CN 106689786 A CN106689786 A CN 106689786A CN 201611189253 A CN201611189253 A CN 201611189253A CN 106689786 A CN106689786 A CN 106689786A
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- feed
- muscular stomach
- weight
- broiler chicken
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- 239000002504 physiological saline solution Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000005019 zein Substances 0.000 description 1
- 229940093612 zein Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
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- Biotechnology (AREA)
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- General Health & Medical Sciences (AREA)
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- Fodder In General (AREA)
Abstract
The invention belongs to the fields of a feed composition and feed processing, particularly relates to a compound feed for promoting the development of the muscular stomach of a broiler, and further provides a preparation method of the feed. The content of crude fibers in percentage by mass of the compound feed is greater than or equal to 4%, and the compound feed is mainly prepared from the following raw materials in parts by weight: 45-60 parts of corn, 3-15 parts of whole wheat grains, 1-3 parts of tropina, 1-5 parts of cottonseed meal, 1-5 parts of oat hull, 0.8-2 parts of calcium hydrogen phosphate, 1-2.5 parts of amino acids, 0.05-0.3 part of sodium humate, 0.05-0.1 part of a mycotoxin adsorbent, 0.02-0.1 part of betaine, 0.1-0.5 part of choline chloride, 0.05-0.3 part of a compound enzyme preparation, 0.5 part of a compound premix, 3-6 parts of fat, and 0.5-5 parts of probiotics. The compound feed disclosed by the invention can notably increase the weight of fresh chicken's gizzards, compared with the weight of each fresh chicken's gizzards of a broiler fed with a common feed, the weight of each fresh chicken's gizzard of the broiler fed with the compound feed is increased by 2-5 grams, and compared with the weight of each fresh chicken's gizzards of a broiler fed with granular feeds, the weight of each fresh chicken's gizzard of the broiler fed with the compound feed is increased by 8-15 grams.
Description
Technical field
The invention belongs to fodder compound and field of feed processing, matching somebody with somebody for broiler chicken muscular stomach development is promoted more particularly to a kind of
Feed is closed, and is related to the preparation method of the feed.
Background technology
Feed granules size influences A＆P feature, the function of digestive system, or even intestinal microecology and pH,
Especially muscular stomach development.Particulate material makes starch gelatinization through high-temperature process in process, and destruction is thin with softening fibre structure
Cell wall, makes protein denaturation, fat stabilization and is conducive to digesting and assimilating.Meanwhile, ANFs activity and toxin are suppressed.Raise
Material raw material sources are wide, and formulation cost is low, and broiler chicken is fast to particulate material feeding, not particular about food, has saved maintenance needs, raises early stage material meat
Than good, breeding cycle is short, and turnover is fast.Have the disadvantage that chicken's gizzard is small, increase on the metabolic disease incidences of disease such as Sudden Death Syndrome, ascites symptoms with the age
Rise, death and culling rate is raised.Meanwhile, late growing stage is also suppressed.Additionally, Feed Manufacturing efficiency is low.Powder, can increase the table of feed
The touch opportunity of area and digestive ferment, so as to improve the utilization rate of nutriment, the death rate is low, and Later growth feedstuff-meat ratio is low, flesh
Stomach is big.Powder has the disadvantage that granularity is small, and Feeding time is (being probably 3 times of particulate material) long, and energy consumption is more, is typically due to chicken feed intake
Deficiency, causes growth potential to give full play to.In broiler chicken production marketing in the market, domestic consumption custom, chicken's gizzard price is relative
It is higher (be higher by about 10 yuan/kilogram with respect to chicken leg, the apex of the wing, big chicken's gizzard than small chicken's gizzard be higher by about 4-8 units/kilogram).Thus promote meat
While chicken growth potential is played, promote muscular stomach development, it is significant to broiler breeding, by increasing capacitance it is possible to increase culture benefit.
For livestock and poultry muscular stomach develop research mainly have it is following these, publication number:The Chinese invention of 105749055A《It is a kind of
For treating the compound traditional Chinese veterinary medicine powder that livestock and poultry are got ill from overeating stagnant》It is a kind of for treat livestock and poultry get ill from overeating stagnant compound traditional Chinese veterinary medicine dissipate
Agent, is made up of following parts by weight of component：Taurine 20-30 parts；Medicated Leaven 20-30 parts；Dusty yeast 10-20 parts；Indigo naturalis 10-20
Part；Malt 10-20 parts；Chinese herbaceous peony 10-20 parts；Compound traditional Chinese veterinary medicine powder of the invention has the lower gas that helps digestion, and appetizing intestines wide cure mainly wound
Dyspepsia is stagnant, indigestion, proventriculitis, the ventriculitis caused for a variety of causes, is mainly shown as that poultry feed intake declines, disappears
Thin, regularity is poor, punishment in advance etc., and the compound traditional Chinese veterinary medicine powder cure rate is high, and feed intake substantially, has no adverse reaction, no drug residue.
Publication number:The Chinese invention of 105724314A《A kind of production method for increasing goose muscular stomach weight》Disclose a kind of increasing
Plus the production method of goose muscular stomach weight, the method comprises the following steps：It is random by body weight from 28 age in days Sichuan White Goose 180
It is divided into 2 groups of control group, 1 group of experiment and experiment, every group of 6 repetitions, each repetition 10；Every group is entered by the way of Diet
Row is raised；It is daily to determine the actual feed intake of control group complete feed, and test group complete feed feed intake is determined on this basis；
Raise to 70 ages in days and terminate.It is contemplated that exploitation is a kind of to increase the low of muscular stomach weight in the case of other production performances are not influenceed
Cost production techniques, the present invention raises 4% adequate diet, but free choice feeding sorghum stalks for 28-70 ages in days Sichuan White Goose in limit
Under the conditions of stalk, its production performance and Slaughter are not influenceed, while significantly improving muscular stomach weight, and possess optimal economic effect
Benefit.
Publication number:The Chinese invention of 102940160A《It is a kind of promote chick gut development poult mixed feed and its
Preparation method》Disclose a kind of poult mixed feed of promotion chick gut development and preparation method thereof.The poult coordinates to be raised
Material is made up of corn, dregs of beans, corn protein powder, the specific raw material rich in insoluble fibre and poult compound premix, wherein
The content of corn is 55-68 parts, dregs of beans 20-28 parts, corn protein powder 3-10 parts, the specific raw material 3- rich in insoluble fibre
6 parts, soybean oil 0-3 parts, 3-6 parts of poult compound premix.Its preparation method is the specificity original that first will be enriched in insoluble fibre
Feed powder is broken to 2-4mm, then corn, dregs of beans are crushed into 1-1.5mm；Each raw material is prepared well mixed in proportion, is finally made
Granularity is 1.5-3.0mm broken materials.This poult mixed feed can significantly improve the development of the digestive organs such as chick muscular stomach, enteron aisle,
Feed digestion utilization rate is improved, chicken production performance is improved, improves chick health status, reduce the disease of digestive tract incidence of disease and dead
Die rate.
Publication number:101606633 Chinese invention《Half granulation of Dorking granular feed and its production method》It is related to bird feed,
Specifically related to half granulation of Dorking granular feed and its production method.The purpose of the present invention is to overcome iblet to be crushed again, then is pressed
Into the shortcoming of particle, saving is pressed into a large amount of electric energy of particle process consumption.Production technology of the invention is：After crush maize,
The thin part that chicken cannot have in the mouth is sifted out come and remaining several composition is pressed into semi-finished granules together with sieve.
The big part of particle on sieve, after being directly sufficiently mixed again uniformly according to the proportioning of formula with the semi-finished granules of above-mentioned extrusion,
It is finished product.The invention has the advantages that：1) energy consumption is low, saves 47% manufacturing cost.So, commercially have pole
Strong competitiveness.2) broiler chicken has eaten this product, and stomach invigorating, disease is few.3) muscular stomach of broiler chicken is bigger, and muscular stomach is most on Asian market
Expensive product.So, this feed can allow final chicken product commercially more competitive.
The content of the invention
The present invention significantly affects broiler chicken gut development according to feed granules size, the characteristics of especially muscular stomach is developed.It is logical
Cross and prove repeatedly, laboratory screening is developed with corn, Intact wheat, oat bran, peanut meal, flour, mycoprotein, compound
Enzyme preparation, compound premix etc. are primary raw material, are crushed using after first dispensing, and roller pulverizer and sieve double combinations are used,
Feed a part raw material cold compression granulating process, designs adequate nutrition level, appropriate particle size, it is adaptable in, big broiler chicken uses
Mixed feed, with reference to particulate material and powder advantage, improves food conversion ratio, and reduces the broiler chicken death rate, promotes growth potential abundant
Performance, increase muscular stomach proportion, can further improve culture benefit.
To solve the above problems, during the present invention provides a kind of can apply to, big broiler chicken stage, antibiotic-free, nutrition
Comprehensively, the mixed feed of uniform granularity, rational feed granularity and hardness, increase the feed residence time in the gastrointestinal tract,
Strengthen muscular stomach and grind feed time and intensity, promote muscular stomach development, reduce metabolic disease hair rate, reduce the death rate, broiler chicken feeding is fast,
It is not particular about food, energy consumption is reduced, feedstuff-meat ratio is reduced, weight percent chicken's gizzard higher can be obtained when butchering, obtain economy higher
Benefit.
To achieve these goals, the technical solution adopted by the present invention is：A kind of cooperation for promoting the development of broiler chicken muscular stomach is raised
Material, is made up of the raw material including following parts by weight：
Corn 45-60 parts, Intact wheat 3-15 parts, dregs of beans 15-25 parts, corn protein powder 1-5 parts, mycoprotein 1-3
Part, cotton dregs 1-5 parts, flour 2-4 parts, oat shell 1-5 parts, stone flour 0.5-2 parts, calcium monohydrogen phosphate 0.8-2 parts, salt 0.2-0.4 parts,
- 2.5 parts of amino acid/11 is sodium humate 0.05-0.3 parts, mold toxin sorbent 0.05-0.1 parts, emulsifying agent 0.02-0.1 parts, sweet
0.02-0.1 parts of dish alkali, Choline Chloride 0.1-0.5 parts, complex enzyme formulation 0.05-0.3 parts, 0.5 part of compound premix, grease 3-6
Part, probiotic 0.5-5 parts.
The mixed feed for promoting the development of broiler chicken muscular stomach, particle diameter more than the 2.8mm shared mass percent in feed
It is 50-60%, the shared mass percent in feed of the particle diameter less than 1mm is less than 2%.
The mixed feed for promoting broiler chicken muscular stomach to develop：Crude fibre mass percentage content >=4%, commercially available existing broiler chicken
Material crude fibre degree is generally 2.5-3.5%.
The probiotic is made up of Lactobacillus plantarum, bacillus licheniformis, saccharomycete, the Lactobacillus plantarum, lichens bud
Spore bacillus, the living bacteria count of saccharomycete are respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, ferment
Female bacterium >=10,000,000,000/g；
Preferably, the yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, protect
It is CGMCC No.12789 to hide numbering；
Preferably, the yeast cultivates preparation by the following method：
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed
Culture 30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Seed liquor is pressed into 10% mass percent inoculum concentration, is accessed in the fermentation tank equipped with 3L fermentation mediums, 30 DEG C, led to
Tolerance 6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably add
Plus the Cys of final concentration of 25mmol/L, total fermentation time is 50h；
Fermentation is finished, and pH is to 6.5 for regulation, is spray-dried to obtain yeast pulvis；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0。
Preferably, the grease is animal fat and/or vegetable fat；The vegetable oil is soya-bean oil, rice bran oil, corn oil
In at least one；The animal oil is at least one in lard, duck oil, chicken fat；
The mycoprotein is at least one in lysine slag, nucleosides acid sludge, glutamic acid slag.
The stone flour directly crushes lime stone, calcite, chalk rock of natural magnesium hydrogen calcium etc., calcium content for mechanical means
36% mass percent is not less than, penicillium toxin 2.8mm above contents are not less than 70% mass percent.The calcium monohydrogen phosphate,
Wherein calcium content is not less than 22% mass percent, and phosphorus content is not less than 16% mass percent.Described Intact wheat be except
The Intact wheat of rigid shell is removed, wheat is feeding wheat, nutritive index：
Crude protein content 10-12% mass percents, crude fat content 1.5-2.2% mass percents, crude fiber content
2-2.5% mass percents, the mass percent of content of starch 55%, 720-750 g/l of unit weight.
The complex enzyme formulation is at least two in zytase, cellulase, mannonase protease, amylase.
Purchased from Shenyang Fengmei Biotechnology Co., Ltd..
Preferably, the amino acid is at least one of lysine, methionine, threonine；
Preferably, the amino acid is made up of the raw material of following parts by weight：Lysine 0.5-0.7 parts, methionine 0.15-
0.3 part, threonine is 0.1-0.3 parts.
The compound premix is produced for Liaoning Hefeng Animal Husbandry Co., Ltd, product authentication code -- and pre- word is raised by the Liao Dynasty
(2013)003065。
Present invention simultaneously provides a kind of preparation method of the mixed feed for promoting broiler chicken muscular stomach to develop, comprise the following steps：
Corn is cut in maize raw material using pair roller pulverizer grinding and sieving, the corn of more than grinding particle size 2.8mm
In shared part by weight be higher than 85%, screenings is maize flour.
Corn quarrel and maize flour and Intact wheat, dregs of beans, corn protein powder, mycoprotein, cotton dregs, flour after crushing,
Oat shell, stone flour, calcium monohydrogen phosphate, salt, amino acid, sodium humate, mold toxin sorbent, emulsifying agent, glycine betaine, chlorination courage
Alkali, complex enzyme formulation, compound premix, probiotic sieving, powder raw material is pelletized using cold granulating process under 2.0mm sieves；Powder
Shape material and its 5% percentage by weight, after the water that sprinkling nebulizes is mixed, normal temperature is 4mm, ring mould from press mold aperture
Compression ratio is 15:1 granulator carries out cold granulation, crushes, and particle size after cracking is 1.5-2.5mm, obtains broken kernel.Broken kernel with
After the mixing of 2.0mm sieves oversize, grease is dissolved into rear oil spout.
Beneficial effect
1st, the raw material such as rational corn particle size after cracking, Intact wheat, oat shell, 20 mesh stone flours increased insoluble in daily ration
Property fiber content, appropriate granularity, extension feed the time is ground in muscular stomach, freshly-slaughtered poultry gizzard weight can be dramatically increased, than feeding powder
Material increases 2-5 grams/piece, and 8-15 grams/piece is increased than fed granules material.
2nd, roller pulverizer and sieve double combinations are used, and the meticulous raw material in part is pelletized using cold compression, reasonably
Feed granularity, reduces feed classification, it is to avoid broiler chicken is particular about food, eats and drinks immoderately, and reduces metabolic disease incidence, especially later stage sudden death
Disease, the generation of ascites symptoms, reduce the death rate 2%.Using cold granulation, without steam regulation of texture, conditioning of texture, reduce to multidimensional, enzyme preparation, micro-
The destruction of ecological agent isoreactivity composition, reduces the powder amount that chicken can not search for food, and reduces chicken Feeding time, it is ensured that
Nutrition is balanced.
3rd, feed nutrition reasonable level, digestible aminoacids balance, crude protein energy balance, cold compression granulation, it is right to reduce
Multidimensional, enzyme preparation, the destruction of probiotics isoreactivity composition, improve nutrition digestion absorption rate, deliver material for sale within 42 days
Meat ratio is reachable:1.86:1.
4th, rational feed granularity, the mass percent higher than 2.8mm particle diameters is 50-60%, less than the quality of 1mm particle diameters
Percentage is less than 2%.Feeding time is reduced, is the 1/2 of powder Feeding time.
5th, rational feed granularity, reduce feeding less than fine powder, the feed that falls earthward etc. of plane, during reducing feeding
Feed waste 1%.
6th, addition probiotic can suppress the propagation of harmful bacteria, promote the propagation of beneficial bacterium, improve small intestine of broiler chickens fine hair
Development, reduces diarrhea rate, improves animal immunizing power；Particularly add the preservation of bacteria strain tlj2016 of high yield glutathione, paddy Guang glycosides
Peptide is such as combined as important antioxidant and free radical scavenger in vivo with free radical, heavy metal, so that having in body
Harmful poisonous substance is converted into harmless material, excretes external, the physical function to improving broiler chicken, very helpful.
Specific embodiment
The Wine brewing yeast strain is specially saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, the bacterium
Strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number on July 15th, 2016
It is CGMCCNo.12789, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica,
Postcode 100101.
The most suitable growth pH of the saccharomyces cerevisiae tlj2016 is 6.0-6.5, and optimum growth temperature is 28-35 DEG C；
The saccharomyces cerevisiae tlj2016 is isolated saccharomyces cerevisiae starting strain from the orchard of one plant of Ningxia through following
Step is obtained：
The original strain that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → hypertonic plate screening → nitrosoguanidine
(NTG) secondary screening (producing glutathione GSH abilities) → passage stabilization is screened → fermented to mutagenesis screening → hypertonic flat board primary dcreening operation → shaking flask
Property experiment.
By the bacterial strain tlj2016 obtained after mutagenesis, its glucose tolerance and Cys tolerance are equal
It is improved, cell density, another aspect Cys tolerance energy on the one hand can be improved under high concentration glucose culture
The raising of power is beneficial to GSH and largely synthesizes in intracellular, so as to improve the ability that bacterial strain mass produces GSH.
Saccharomyces cerevisiae of the present invention reaches 300g/L to the tolerance of glucose, beneficial to it in high concentration grape
GSH is produced under the conditions of sugar；3308mg/L is reached in 5L fermentation cylinder for fermentation production GSH final concentrations；Tolerate the energy of Cys
Power is far above starting strain, slow growth is remained under the effect of 5mmol/L Cys, in 40mmol/LL- cysteines
Remain to keep GSH largely to synthesize under effect；Salt resistance ability reaches 18%, is conducive to extending its application field.
1.DES mutagenic and breedings
1) ring of starting strain 1 one on test tube slant is taken on super-clean bench, is accessed equipped with 50mL malt extract mediums
In 250mL triangular flasks, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.
2) 5mL bacterium solutions are taken, 5000rpm centrifugation 10min collects thallines, with brine 2 times.
3) 10 are diluted to pH7.0 phosphate buffers7Individual/mL bacteria suspensions.
4) kaliumphosphate buffer of 32mL pH7.0,8mL bacteria suspensions, 0.4mL DES are taken and is being placed in advance in the 150mL of rotor
It is sufficiently mixed in triangular flask, makes DES ultimate densities be 1% (v/v).
5) the 150rpm reactions 30min in 30 DEG C of shaking tables, takes 1mL mixed liquors, adds 0.5mL 25%Na2S2O3In solution
Only react.
6) dilution spread is in the malt extract medium plate containing 150g/L KCl, the picking bacterium after 30 DEG C of cultures 2-3 days
The bacterial strain 1 of the maximum that falls.
2. nitrosoguanidine mutagenesis
1) ring of saccharomyces cerevisiae bacteria strain 1 one on test tube slant is taken on super-clean bench, is accessed and 50mL brewer's wort cultures is housed
In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.
2) 5mL bacterium solutions 5000rpm centrifugation 10min collects thallines are taken, with brine 2 times.
3) 10 are diluted to pH6.0 phosphate buffers7Individual/mL bacteria suspensions.
4) 10mL bacteria suspensions are taken to be transferred in 100mL triangular flasks, the NTG of 10mg is added, final concentration of 10mg/mL is configured to
NTG solution, and add 4-5 drop acetone, be beneficial to NTG dissolvings.
5) the 200rpm oscillating reactions 30min at 30 DEG C, 5000rpm are centrifuged 10min collects thallines, use SPSS
Wash for several times, stopped reaction.
6) appropriate dilution spread, takes the bacterium solution 0.2mL of last dilution factor, coats the brewer's wort culture containing 200g/L KCl
In base plate.Picking colony 20 after being cultivated 2-3 days at 30 DEG C.
3. shaking flask primary dcreening operation
1) take above-mentioned each ring of 20 S. cervisiaes respectively on super-clean bench, be respectively connected to equipped with 50mL brewer's wort cultures
In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 12h or so, thalline is in mid log phase.
2) 5mL bacterium solutions are taken, is accessed equipped with the 250mL in the hypertonic malt extract mediums of 50mL (concentration of glucose is 300g/L)
In triangular flask, 200rpm, 30 DEG C are cultivated 3-4 days, daily detection concentration of glucose and concentration of alcohol change.After fermentation ends, than
Compared with 20 plants of glucose and ethanol wear rate of strain, final remaining sugar concentration and concentration of alcohol, glucose to the conversion ratio of ethanol
And heteroacid content.
3) glucose consumption rate is fast, final remaining sugar concentration is low and concentration of alcohol is high 5 plants of bacterium are named as Y-1, Y- for selection
2, Y-3, Y-4, Y-5.
4. ferment secondary screening
By 5 plants of bacterium Y-1, Y-2, Y-3, Y-4, Y-5 and starting strain of gained in step 3, respectively according to 10% inoculation
Amount, the 150rpm in the 250mL shaking flasks equipped with 30mL fluid nutrient mediums, 30 DEG C of culture 30h, takes zymotic fluid and determines GSH concentration；
Fluid nutrient medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1；
GSH assay methods：Fresh yeast, at 30 DEG C, 40% Ethanol Treatment 3h, in centrifuging and taking are obtained after zymotic fluid centrifuge washing
Clear liquid is cooked GSH determination samples；
GSH measure is carried out using alloxan method：Its principle is that-the SH on GSH reacts with alloxan, the material of generation
There is absworption peak at 305nm, and it is linear with glutathione concentrations, therefore can be quantitative determined with ultraviolet specrophotometer
GSH contents.
Table 1：GSH testing results
| Bacterial strain | Starting strain | Y-1 | Y-2 | Y-3 | Y-4 | Y-5 |
| GSH concentration (mg/L) | 127.9 | 195.7 | 172.3 | 263.2 | 216.5 | 185.2 |
As seen from the results in Table 1, bacterial strain Y-3 has highest GSH fermentabilities, it is thus determined that Y-3 is final production bacterium
Strain, and it is named as tlj2016.
5. genetic stability experiment
Continuous ten passages on inclined-plane by tlj2016 bacterium, and detect the hair after passage every time with the method for shaking flask secondary screening
Ferment situation.Experiment finds that continuous ten passages on inclined-plane, the strain proterties does not have significant change, and property indices are all just
Often, illustrate that the genetic stability of the strain is stronger.
Fermented under the conditions of saccharomyces cerevisiae tlj2016 sugar high and produce GSH capacity experimentals
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed
Culture 30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Seed liquor is pressed into 10% inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, throughput 6L/
Min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably add final concentration
It is the Cys of 25mmol/L, total fermentation time is 50h；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0；
After fermentation ends, the content for determining GSH in zymotic fluid is 3308mg/L.
Saccharomyces cerevisiae tlj2016L- cysteines tolerance is tested
By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to
150rpm in shaking flask, 30 DEG C are cultivated, when culture is to 12h, to the Guang ammonia of L- half that different final concentrations are added in shaking flask
Acid, is further cultured for 10h, determines dry cell weight, as a result table 2,3；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 20, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
Table 2：Starting strain Cys tolerance
| Cys concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
| Starting strain dry weight g/L | 22.6 | 15.7 | 10.2 | 4.3 | 2.2 | 0.8 |
| GSH concentration (mg/L) | 35.6 | 46.7 | 43.2 | 40.7 | 37.9 | 25.3 |
Table 3：Tlj2016L- cysteine tolerances
| Cys concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
| Tlj2016 dry weights g/L | 25.7 | 28.5 | 23.6 | 21.2 | 20.6 | 18.7 |
| GSH concentration (mg/L) | 73.2 | 98.3 | 113.5 | 121.7 | 127.5 | 135.8 |
From the results shown in Table 2, for starting strain, Cys are added in culture medium, cell stops growing,
And start self-dissolving, cause GSH growth rates to be reduced with the rising of Cys concentration；As can be seen from Table 3, it is low
Under concentration Cys, tlj2016 still is able to slow growth, with the raising of Cys concentration, tlj2016 bacterial strains
Dry cell weight slowly declines, and GSH concentration sustainable growths, and this result is beneficial in GSH production processes by adding precursor
Amino acid-Cys promote the production of GSH.
Saccharomyces cerevisiae tlj2016 salt resistance abilities are tested
Take tlj2016 bacterium solutions 1mL inoculation strain in containing different NaCl concentrations (concentration gradients be 0%, 2%, 5%,
10%th, 15%, 10mL YPD fluid nutrient mediums (pH=6.5) 18%), 24h is cultivated at being placed in 30 DEG C respectively, each treatment 3
Individual repetition.Respectively take 1ml samples bacterium solution to be mixed in 9ml physiological saline, prepare dilution factor solution, take 0.1ml dilutions solid in YPD
It is coated with body flat board, culture 36 hours (each dilution factor do 3 parallel) record is inverted in 30 DEG C of biochemical cultivation cases and calculates flat
Bacterium number number on plate.The results are shown in Table 4, it is known that the resistance to salinity of the bacterium is 18%, illustrates that tlj2016 not only can be in conventional ring
Survived in border, still there is vigor under high salt conditions, can be applied to consume sugar in the high salt food processing process such as soy sauce, curing food
Produce glutathione.
Table 4：Salt resistance ability detection (× 107cfu/ml)
| NaCl contents | 0% | 2% | 5% | 10% | 15% | 18% |
| Original strain | 5.16±0.42 | 4.38±0.42 | 2.15±0.21 | 0.12±0.11 | 0 | 0 |
| tlj2016 | 5.33±0.28 | 5.10±0.71 | 4.83±0.42 | 3.98±0.33 | 2.57±0.48 | 0.83±0.15 |
Embodiment 1
A kind of mixed feed for promoting broiler chicken muscular stomach to develop, is prepared from the following raw materials in parts by weight：
56.3 parts of corn, 3 parts of Intact wheat, 20 parts of dregs of beans, 4 parts of corn protein powder, 2 parts of nucleosides acid sludge, 2 parts of cotton dregs, face
2 parts of powder, 2 parts of oat shell, 1 part of calcium monohydrogen phosphate, 1.15 parts of stone flour, 0.3 part of salt, 1 part of lysine sulphate, DL- METHIONINE 0.3
Part, 0.14 part of threonine, 0.1 part of sodium humate, 0.05 part of mold toxin sorbent, 0.02 part of emulsifying agent, 0.03 part of glycine betaine,
0.1 part of Choline Chloride, 0.1 part of complex enzyme formulation, 0.5 part of compound premix, 2 parts of poultry fat, 1.5 parts of rice bran oil, probiotic
0.5 part.
The mixed feed for promoting the development of broiler chicken muscular stomach, particle diameter more than the 2.8mm shared mass percent in feed
It is 55%, the shared mass percent in feed of the particle diameter less than 1mm is less than 2%.
The mixed feed for promoting broiler chicken muscular stomach to develop：Crude fibre mass percentage content >=4%；
The probiotic is made up of Lactobacillus plantarum, bacillus licheniformis, saccharomycete, the Lactobacillus plantarum, lichens bud
Spore bacillus, the living bacteria count of saccharomycete are respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, ferment
Female bacterium >=10,000,000,000/g；
The yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number
It is CGMCC No.12789；
The yeast cultivates preparation by the following method：
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed
Culture 30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
By seed liquor by the inoculum concentration of 10% mass percent, access and be equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C,
Throughput 6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably
The Cys of final concentration of 25mmol/L are added, total fermentation time is 50h；
Fermentation is finished, and pH is to 6.5 for regulation, is spray-dried to obtain yeast pulvis；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0。
The stone flour directly crushes lime stone, calcite, chalk rock of natural magnesium hydrogen calcium etc., calcareous amount for mechanical means
Degree is not less than 36%, and penicillium toxin 2.8mm above mass percents are not less than 70%.
The calcium monohydrogen phosphate, wherein calcium mass percentage content are not less than 22%, and phosphorus mass percentage content is not less than
16%.
Described Intact wheat is the Intact wheat for removing rigid shell, and wheat is feeding wheat, nutritive index：
Crude protein mass percentage content 10-12%, crude fat mass percentage content 1.5-2.2%, crude fibre quality
Degree 2-2.5%, starch quality degree 55%, 720-750 g/l of unit weight.
Promote the preparation method of the mixed feed of broiler chicken muscular stomach development, comprise the following steps：
Corn is cut in maize raw material using pair roller pulverizer grinding and sieving, the corn of more than grinding particle size 2.8mm
In shared part by weight be higher than 85%；Screenings is maize flour；
Corn quarrel and maize flour and Intact wheat, dregs of beans, corn protein powder, mycoprotein, cotton dregs, flour after crushing,
Oat shell, stone flour, calcium monohydrogen phosphate, salt, amino acid, sodium humate, mold toxin sorbent, emulsifying agent, glycine betaine, chlorination courage
Alkali, complex enzyme formulation, compound premix, probiotic sieving, powder raw material is pelletized using cold granulating process under 2.0mm sieves；Powder
It is 4mm, ring mold compression from press mold aperture after the water that 5% sprinkling nebulizes is mixed that shape raw material and its percentage by weight are
Than being 15:1 granulator carries out cold granulation, crushes, and particle size after cracking is 2.0mm, obtains broken kernel.Broken kernel is sieved with 2.0mm sieves
After upper thing mixing, oil spout.
Embodiment 2
A kind of mixed feed for promoting broiler chicken muscular stomach to develop, is prepared from the following raw materials in parts by weight：
51.3 parts of corn, 5 parts of Intact wheat, 19 parts of dregs of beans, 1 part of corn protein powder, 2 parts of glutamic acid slag, 5 parts of cotton dregs, face
2 parts of powder, 3 parts of oat shell, 1.1 parts of calcium monohydrogen phosphate, 1.3 parts of stone flour, 0.3 part of salt, 0.63 part of lysine sulphate, DL- egg ammonia
Sour 0.25 part, 0.12 part of threonine, 0.2 part of sodium humate, 0.05 part of mold toxin sorbent, 0.03 part of emulsifying agent, glycine betaine
0.02 part, 0.1 part of Choline Chloride, 0.1 part of complex enzyme formulation, 0.5 part of compound premix, 4 parts of poultry fat, 2 parts of soybean oil, benefit
Raw 1 part of element.
The mixed feed for promoting the development of broiler chicken muscular stomach, particle diameter more than the 2.8mm shared mass percent in feed
It is 50%, the shared mass percent in feed of the particle diameter less than 1mm is less than 2%.
The mixed feed for promoting broiler chicken muscular stomach to develop：Crude fibre mass percentage content >=4%；
The probiotic is made up of Lactobacillus plantarum, bacillus licheniformis, saccharomycete, the Lactobacillus plantarum, lichens bud
Spore bacillus, the living bacteria count of saccharomycete are respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, ferment
Female bacterium >=10,000,000,000/g；
The yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number
It is CGMCC No.12789；
The Yeast Cultivation preparation method is with embodiment 1；
The stone flour directly crushes lime stone, calcite, chalk rock of natural magnesium hydrogen calcium etc., calcareous amount for mechanical means
Degree is not less than 36%, and penicillium toxin 2.8mm above mass percentage contents are not less than 70%.
The calcium monohydrogen phosphate, wherein calcium mass percentage content are not less than 22%, and phosphorus mass percentage content is not less than
16%.
Described Intact wheat is the Intact wheat for removing rigid shell, and wheat is feeding wheat, nutritive index：
Crude protein mass percentage content 10-12%, crude fat mass percentage content 1.5-2.2%, crude fibre quality
Degree 2-2.5%, starch quality degree 55%, 720-750 g/l of unit weight.
Promote the preparation method of the mixed feed of broiler chicken muscular stomach development, comprise the following steps：
Corn is cut in maize raw material using pair roller pulverizer grinding and sieving, the corn of more than grinding particle size 2.8mm
In shared part by weight be higher than 85%；Screenings is maize flour；
Corn quarrel and maize flour after crushing are sieved with other raw materials, and powder raw material uses cold granulation work under 2.0mm sieves
Skill is pelletized；It is 4mm, ring from press mold aperture after the water that powder raw material nebulizes with the sprinkling of its percentage by weight 5% is mixed
Mold compression ratio is 15:1 granulator carries out cold granulation, crushes, and particle size after cracking is 2.5mm, obtains broken kernel.Broken kernel and 2.0mm
After the mixing of sieve oversize, grease is dissolved into rear oil spout.
Embodiment 3
A kind of mixed feed for promoting broiler chicken muscular stomach to develop, is prepared from the following raw materials in parts by weight：
53.3 parts of corn, 3 parts of Intact wheat, 18 parts of dregs of beans, 2.5 parts of corn protein powder, 3 parts of glutamic acid slag, 1 part of cotton dregs,
2 parts of flour, 2 parts of oat shell, 2 parts of calcium monohydrogen phosphate, 0.8 part of stone flour, 0.2 part of salt, 0.74 part of FE-5, DL- egg ammonia
Sour 0.2 part, 0.14 part of threonine, 0.3 part of sodium humate, 0.05 part of mold toxin sorbent, 0.05 part of emulsifying agent, glycine betaine
0.02 part, 0.1 part of Choline Chloride, 0.1 part of complex enzyme formulation, 0.5 part of compound premix, 3.5 parts of poultry fat, corn oil 1.5
Part, 5 parts of probiotic.
The mixed feed for promoting the development of broiler chicken muscular stomach, particle diameter more than the 2.8mm shared mass percent in feed
It is 60%, the shared mass percent in feed of the particle diameter less than 1mm is less than 2%.
The mixed feed for promoting broiler chicken muscular stomach to develop：Crude fibre mass percentage content >=4%；
The probiotic is made up of Lactobacillus plantarum, bacillus licheniformis, saccharomycete, the Lactobacillus plantarum, lichens bud
Spore bacillus, the living bacteria count of saccharomycete are respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, ferment
Female bacterium >=10,000,000,000/g；
The yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number
It is CGMCC No.12789；
The Yeast Cultivation preparation method is with embodiment 1；
The stone flour directly crushes lime stone, calcite, chalk rock of natural magnesium hydrogen calcium etc., calcareous amount for mechanical means
Degree is not less than 36%, and penicillium toxin 2.8mm above mass percentage contents are not less than 70%.
The calcium monohydrogen phosphate, wherein calcium mass percentage content are not less than 22%, and phosphorus mass percentage content is not less than
16%.
Described Intact wheat is the Intact wheat for removing rigid shell, and wheat is feeding wheat, nutritive index：
Crude protein mass percentage content 10-12%, crude fat mass percentage content 1.5-2.2%, crude fibre quality
Degree 2-2.5%, starch quality degree 55%, 720-750 g/l of unit weight.
Promote the preparation method of the mixed feed of broiler chicken muscular stomach development, comprise the following steps：
Corn is cut in maize raw material using pair roller pulverizer grinding and sieving, the corn of more than grinding particle size 2.8mm
In shared part by weight be higher than 85%；Screenings is maize flour；
Corn quarrel and maize flour after crushing are sieved with other raw materials, and powder raw material uses cold granulation work under 2.0mm sieves
Skill is pelletized；Powder raw material and its 5% percentage by weight, are 4mm from press mold aperture after the water that sprinkling nebulizes is mixed,
Ring mold compression ratio is 15:1 granulator carries out cold granulation, crushes, and particle size after cracking is 1.5mm, obtains broken kernel.Broken kernel with
After the mixing of 2.0mm sieves oversize, grease is dissolved into rear oil spout.
Embodiment 4
A kind of mixed feed for promoting broiler chicken muscular stomach to develop, is made up of the raw material including following parts by weight：
50.39 parts of corn, 4 parts of Intact wheat, 25 parts of dregs of beans, 1 part of corn protein powder, 1 part of lysine slag, 1 part of cotton dregs, face
3 parts of powder, 5 parts of oat shell, 2 parts of stone flour, 0.8 part of calcium monohydrogen phosphate, 0.1 part of salt, 0.9 part of FE-5, DL- METHIONINE
0.4 part, 0.34 part of threonine, 0.3 part of sodium humate, 0.05 part of mold toxin sorbent, 0.05 part of emulsifying agent, glycine betaine 0.07
Part, 0.3 part of Choline Chloride, 0.3 part of complex enzyme formulation, 0.5 part of compound premix, 3 parts of corn oil, 0.5 part of probiotic.
The mixed feed for promoting the development of broiler chicken muscular stomach, particle diameter more than the 2.8mm shared mass percent in feed
It is 60%, the shared mass percent in feed of the particle diameter less than 1mm is less than 2%.
The mixed feed for promoting broiler chicken muscular stomach to develop：Crude fibre mass percentage content >=4%；
The probiotic is made up of Lactobacillus plantarum, bacillus licheniformis, saccharomycete, the Lactobacillus plantarum, lichens bud
Spore bacillus, the living bacteria count of saccharomycete are respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, ferment
Female bacterium >=10,000,000,000/g；
The yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number
It is CGMCC No.12789；
The Yeast Cultivation preparation method is with embodiment 1；
The stone flour directly crushes lime stone, calcite, chalk rock of natural magnesium hydrogen calcium etc., calcareous amount for mechanical means
Degree is not less than 36%, and penicillium toxin 2.8mm above mass percentage contents are not less than 70%.
The calcium monohydrogen phosphate, wherein calcium mass percentage content are not less than 22%, and phosphorus mass percentage content is not less than
16%.
Described Intact wheat is the Intact wheat for removing rigid shell, and wheat is feeding wheat, nutritive index：
Crude protein mass percentage content 10%, crude fat mass percentage content 2.2%, crude fibre mass percent contains
Amount 2%, starch quality degree 55%, 750 g/l of unit weight.
Promote the preparation method of mixed feed of broiler chicken muscular stomach development with embodiment 1.
Embodiment 5
A kind of mixed feed for promoting broiler chicken muscular stomach to develop, is made up of the raw material including following parts by weight：
49.5 parts of corn, 8 parts of Intact wheat, 15 parts of dregs of beans, 5 parts of corn protein powder, 3 parts of glutamic acid slag, 2 parts of cotton dregs, face
2 parts of powder, 1 part of oat shell, 0.8 part of stone flour, 1.2 parts of calcium monohydrogen phosphate, 0.4 part of salt, 1.3 parts of lysine sulphate, DL- METHIONINE
0.7 part, 0.5 part of threonine, 0.05 part of sodium humate, 0.1 part of mold toxin sorbent, 0.1 part of emulsifying agent, glycine betaine 0.05
Part, 0.5 part of Choline Chloride, 0.3 part of complex enzyme formulation, 0.5 part of compound premix, 6 parts of rice bran oil, 2 parts of probiotic.
The mixed feed for promoting the development of broiler chicken muscular stomach, particle diameter more than the 2.8mm shared mass percent in feed
For 50% particle diameter less than 1mm, shared mass percent is less than 2% in feed.The cooperation for promoting the development of broiler chicken muscular stomach is raised
Material：Crude fibre mass percentage content 4%；
The probiotic is made up of Lactobacillus plantarum, bacillus licheniformis, saccharomycete, the Lactobacillus plantarum, lichens bud
Spore bacillus, the living bacteria count of saccharomycete are respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, ferment
Female bacterium >=10,000,000,000/g；
The yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number
It is CGMCC No.12789；
The yeast is prepared by this area conventional method culture；
The stone flour directly crushes lime stone, calcite, chalk rock of natural magnesium hydrogen calcium etc., calcareous amount for mechanical means
Degree is not less than 36%, and penicillium toxin 2.8mm above mass percentage contents are not less than 70%.
The calcium monohydrogen phosphate, wherein calcium mass percentage content are not less than 22%, and phosphorus mass percentage content is not less than
16%.
Described Intact wheat is the Intact wheat for removing rigid shell, and wheat is feeding wheat, nutritive index：
Crude protein mass percentage content 10-12%, crude fat mass percentage content 1.5-2.2%, crude fibre quality
Degree 2-2.5%, starch quality degree 55%, 720-750 g/l of unit weight.
Promote the preparation method of the mixed feed of broiler chicken muscular stomach development, with embodiment 2.
Embodiment 6
A kind of mixed feed for promoting broiler chicken muscular stomach to develop, is made up of the raw material including following parts by weight：
45 parts of corn, 15 parts of Intact wheat, 17 parts of dregs of beans, 3 parts of zein, 3 parts of glutamic acid slag, 1 part of cotton dregs, flour 4
Part, 2 parts of oat shell, 0.5 part of stone flour, 1.2 parts of calcium monohydrogen phosphate, 0.3 part of salt, 1.1 parts of lysine sulphate, DL- METHIONINE 0.7
Part, threonine 0.2,0.05 part of sodium humate, 0.05 part of mold toxin sorbent, 0.03 part of emulsifying agent, 0.02 part of glycine betaine, chlorine
Change 0.3 part of choline, 0.05 part of complex enzyme formulation, 0.5 part of compound premix, 3 parts of rice bran oil, 1.5 parts of duck oil, 0.5 part of probiotic.
The mixed feed for promoting the development of broiler chicken muscular stomach, particle diameter more than the 2.8mm shared mass percent in feed
For 50% particle diameter less than 1mm, shared mass percent is less than 2% in feed.
The mixed feed for promoting broiler chicken muscular stomach to develop：Crude fibre mass percentage content >=4%；
The probiotic is made up of Lactobacillus plantarum, bacillus licheniformis, saccharomycete, the Lactobacillus plantarum, lichens bud
Spore bacillus, the living bacteria count of saccharomycete are respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, ferment
Female bacterium >=10,000,000,000/g；
The stone flour directly crushes lime stone, calcite, chalk rock of natural magnesium hydrogen calcium etc., calcareous amount for mechanical means
Degree is not less than 36%, and penicillium toxin 2.8mm above mass percentage contents are not less than 70%.
The calcium monohydrogen phosphate, wherein calcium mass percentage content are not less than 22%, and phosphorus mass percentage content is not less than
16%.
Described Intact wheat is the Intact wheat for removing rigid shell, and wheat is feeding wheat, nutritive index：
Crude protein mass percentage content 10-12%, crude fat mass percentage content 1.5-2.2%, crude fibre quality
Degree 2-2.5%, starch quality degree 55%, 720-750 g/l of unit weight.
Embodiment 7
A kind of mixed feed for promoting broiler chicken muscular stomach to develop, is made up of the raw material including following parts by weight：
60 parts of corn, 3 parts of Intact wheat, 17.86 parts of dregs of beans, 2 parts of corn protein powder, 1 part of glutamic acid slag, lysine slag 1
Part, 1 part of nucleosides acid sludge, 1 part of cotton dregs, 2 parts of flour, 1 part of oat shell, 1 part of stone flour, 1.1 parts of calcium monohydrogen phosphate, 0.4 part of salt relies ammonia
0.70 part of acid hydrochloride, 0.31 part of DL- METHIONINE, threonine 0.13,0.15 part of sodium humate, 0.1 part of mold toxin sorbent,
0.05 part of emulsifying agent, 0.1 part of glycine betaine, 0.4 part of Choline Chloride, 0.2 part of complex enzyme formulation, 0.5 part of compound premix, soya-bean oil 2
Part, 1 part of corn oil, 1 part of rice bran oil, 1 part of chicken fat, 1 part of lard, 1 part of probiotic.
The other the same as in Example 1.
In above-mentioned case study on implementation 1-7：
Compound premix is produced for Liaoning Hefeng Animal Husbandry Co., Ltd, product authentication code -- and pre- word (2013) is raised by the Liao Dynasty
003065, product form is B B-complex and composite trace element, and trade mark is that standing grain is rich.
Complex enzyme formulation is Shenyang Fengmei Biotechnology Co., Ltd.'s production, main component zytase, cellulase, sweet
Dew dextranase, protease, amylase.Registration mark is lush 901.
Emulsifying agent is produced to build bright industry (Zhuhai) Co., Ltd, main component lysophosphatidyl choline (haemolysis lecithin
Fat).Registration mark is the easypro treasured LYSOFORTE of profit.
Test example：
The mixed feed for promoting muscular stomach to develop prepared using above-described embodiment, does following checking：
1st, experiment material
1.1 experimental periods：In September, 2015-November, last 50 days
1.2 experiment places：Daliang City Wafangdian broiler chicken factory
1.3 experimental animals：Experimental animal selects white meat-type chickens 6000.
2nd, experimental technique
2.1 experimental designs：Tested in 12 days 25 days-November of September in 2015, in Dalian Wafangdian, broiler trial is carried out.
Experiment is carried out 42 days, selects 6000 white meat-type chickens, uses single factor design, if 5 treatment, 2 repetitions, each repetition
600.0-11 ages in days feed same chick broken material.12-14 age in days transition feeds, 15 ages in days start to feed cooperation of the present invention
Feed.Testing material is grouped as follows：
2.2 feedings and managements：
Feeding method：The transitional period reloaded for 4 days, chick broken material successively decreases according to the 25% of scale of feeding daily, and testing material is every
It increases according to the 25% of scale of feeding, free choice feeding.15-21 ages in days feed 2-4 times/day according to feed intake；22-42 ages in days according to
3-4 times/day of feed intake fed standard.Feed intake in recommendation of new cultivars standard according to feed every time when charging tray remaining material situation slightly
Adjustment, each and every one hour charging tray of feed first half is empty each time.
Hen house temperature：
More than 42 grams 33 degree of brooding temperatures, progressively lower the temperature, and 0.5 degree are dropped daily within first 7 days, drop 0.3 degree after 7 days daily, always
To delivering for sale.
Hen house humidity：1-7 days 65-70%, 8 days-to deliver for sale be 50-60%.
Illumination program：It is within 1st day illumination in 24 hours, is within 2-7 days 23 hours, 8-21 days is illumination in 18 hours, is within 22-28 days
Illumination in 20 hours, 29 days-to deliver for sale be illumination in 23 hours.Dark initial time is selected in the natural interlunation of one day, when dark
Between be it is continuous, uninterruptedly.
Intensity of illumination：
0-6 ages in days are 30-45 luxs；7-21 ages in days are 5-10 luxs；22 ages in days-deliver for sale are 10 luxs.
Coop ventilation：
1-21 ages in days, are divulged information, it is to avoid harmful wind using minimum ventilation rate mode；22-28 ages in days are divulged information using transient mode；
29- delivers for sale, using longitudinal ventilation.According to the appropriate adjustment of chicken performance, it is ensured that ammonia gas level in hen house<10ppm, dense carbon dioxide
Degree<0.3%.
2.3 testing indexs：
Record 15-42 ages in days, every group of chicken feed intake, dead chicken number, 42 age in days early morning empty body weights butcher latter every group
The total fresh weight of chicken chicken's gizzard.
Calculate：Feedstuff-meat ratio, survival rate, chicken's gizzard account for percentage by weight and performance analysis
2.4 data processings
Test data is analyzed using EXEL2007.
Table 5：Influence of the present invention to meat chicken production performance
Result shows that using mixed feed of the present invention feeding 15-42 Day-old Broiler Chickens, can reduce feedstuff-meat ratio, raising is delivered for sale
Average weight, increases survival rate, improves fresh muscular stomach counterpoise, reduces the incidence of disease, reduces drug cost, improves the product of broiler chicken and muscular stomach
Matter, promotes giving full play to for meat chicken production performance, reduces aquaculture cost, increases culture benefit.
Claims (10)
1. a kind of mixed feed for promoting broiler chicken muscular stomach to develop, is made up of the raw material including following parts by weight：
Corn 45-60 parts, Intact wheat 3-15 parts, dregs of beans 15-25 parts, corn protein powder 1-5 parts, mycoprotein 1-3 parts, cotton dregs
1-5 parts, flour 2-4 parts, oat shell 1-5 parts, stone flour 0.5-2 parts, calcium monohydrogen phosphate 0.8-2 parts, salt 0.2-0.4 parts, amino acid
1-2.5 parts, sodium humate 0.05-0.3 parts, mold toxin sorbent 0.05-0.1 parts, emulsifying agent 0.02-0.1 parts, glycine betaine
0.02-0.1 parts, Choline Chloride 0.1-0.5 parts, complex enzyme formulation 0.05-0.3 parts, 0.5 part of compound premix, grease 3-6 parts,
Probiotic 0.5-5 parts；
Characterized in that, crude fibre mass percentage content >=4%.
2. the mixed feed of broiler chicken muscular stomach development is promoted according to claim 1, it is characterised in that the particle diameter more than 2.8mm
Shared mass percent is 50-60% in feed, and the shared mass percent in feed of the particle diameter less than 1mm is less than 2%.
3. the mixed feed of broiler chicken muscular stomach development is promoted according to claim 1, it is characterised in that the probiotic is by plant
Lactobacillus, bacillus licheniformis, saccharomycete composition, the Lactobacillus plantarum, bacillus licheniformis, the living bacteria count of saccharomycete
Respectively：Lactobacillus plantarum >=10,000,000,000/g, bacillus licheniformis >=15,000,000,000/g, saccharomycete >=10,000,000,000/g.
4. the mixed feed of broiler chicken muscular stomach development is promoted according to claim 3, it is characterised in that the saccharomycete is preservation
Strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number is CGMCC No.12789.
5. the mixed feed of broiler chicken muscular stomach development is promoted according to claim 4, it is characterised in that the saccharomycete is by as follows
It is prepared by method culture：
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm in the 250mL shaking flasks equipped with 30mL Shake flask mediums, 30 DEG C of cultures is accessed
30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Seed liquor is pressed into 10% mass percent inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, throughput
6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, and disposable addition is eventually
Concentration is the Cys of 25mmol/L, and total fermentation time is 50h；
Fermentation is finished, and pH is to 6.5 for regulation, is spray-dried to obtain yeast pulvis；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0。
6. the mixed feed of broiler chicken muscular stomach development is promoted according to claim 1, it is characterised in that the grease is
Thing grease and/or vegetable fat；The vegetable oil is at least one in soya-bean oil, rice bran oil, corn oil；The animal
Oil is at least one in lard, duck oil, chicken fat.
7. the mixed feed of broiler chicken muscular stomach development is promoted according to claim 1, it is characterised in that the mycoprotein is bad
At least one in propylhomoserin slag, nucleosides acid sludge, glutamic acid slag.
8. the mixed feed of broiler chicken muscular stomach development is promoted according to claim 1, it is characterised in that the stone flour is machinery side
Method directly crushes lime stone, calcite, the chalk rock of natural magnesium hydrogen calcium, and calcium mass percentage content is not less than 36%, stone flour
Granularity 2.8mm above mass percentage contents are not less than 70%.
9. the mixed feed of broiler chicken muscular stomach development is promoted according to claim 1, it is characterised in that the amino acid is by as follows
The raw material composition of parts by weight：Lysine 0.5-0.7 parts, methionine 0.15-0.3 parts, threonine is 0.1-0.3 parts.
10. the preparation method of any mixed feeds for promoting broiler chicken muscular stomach to develop of claim 1-9, including following step are prepared
Suddenly：
Corn is using pair roller pulverizer grinding and sieving, corn quarrel institute in maize raw material of more than grinding particle size 2.8mm
Part by weight is accounted for higher than 85%, screenings is maize flour；
Corn quarrel and maize flour and Intact wheat, dregs of beans, corn protein powder, mycoprotein, cotton dregs, flour, oat after crushing
Shell, stone flour, calcium monohydrogen phosphate, salt, amino acid, sodium humate, mold toxin sorbent, emulsifying agent, glycine betaine, Choline Chloride,
Complex enzyme formulation, compound premix, probiotic sieving, powder raw material is pelletized using cold granulating process under 2.0mm sieves；By powdery
After the water that 5% sprinkling of weight of material percentage nebulizes is mixed, normal temperature is 4mm, ring mold compression ratio from press mold aperture
It is 15:1 granulator carries out cold granulation, crushes, and particle size after cracking is 1.5-2.5mm, obtains broken kernel；Broken kernel and 2.0mm sieves
After oversize mixing, grease is dissolved into rear oil spout.
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| CN107912360A (en) * | 2017-11-09 | 2018-04-17 | 辽宁禾丰牧业股份有限公司 | A kind of white meat-type chickens feeding pattern |
| CN109123199A (en) * | 2018-10-17 | 2019-01-04 | 邱榕生 | A kind of feed that can stimulate broiler chicken gut development |
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| CN110692850A (en) * | 2019-10-09 | 2020-01-17 | 北京首朗生物科技有限公司 | Application of clostridium ethanolate protein in poultry feed |
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| CN112674232A (en) * | 2020-12-29 | 2021-04-20 | 喀什泰昆饲料有限责任公司 | Feed for improving muscular stomach development of yellow-feathered broilers and preparation method and application thereof |
| CN115517321A (en) * | 2021-05-25 | 2022-12-27 | 山东德聚仁合生物工程有限公司 | Preparation method for improving proventriculitis and muscular gastritis of poultry by using natural feed raw materials |
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Application publication date: 20170524 |