CN109362948A - A method of Se-enriched feedstuff additive is prepared using Moringa waste wood - Google Patents
A method of Se-enriched feedstuff additive is prepared using Moringa waste wood Download PDFInfo
- Publication number
- CN109362948A CN109362948A CN201811234966.6A CN201811234966A CN109362948A CN 109362948 A CN109362948 A CN 109362948A CN 201811234966 A CN201811234966 A CN 201811234966A CN 109362948 A CN109362948 A CN 109362948A
- Authority
- CN
- China
- Prior art keywords
- parts
- moringa
- selenium
- rich
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Animal Husbandry (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Birds (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to feed addictive processing technique field, specifically a kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood.The following steps are included: (1) mechanical activation pre-processes: being crushed to obtain selenium-rich moringa powder after taking the discarded wooden branch of selenium-rich Moringa and selenium-rich leaf of Moringa to dry, selenium-rich moringa powder, high aluminium ball, Chinese herbal medicine solution are added in the ball mill, adjusting drum's speed of rotation, water bath with thermostatic control temperature are taken out after carrying out 2 ~ 3h of activation, by selenium-rich moringa powder and the isolated activation selenium-rich moringa powder of zirconia ball grinding media progress after treatment；(2) fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, composite bacteria, glucose, lactose, it finally adds sterile water adjusting material-water ratio and is sealed fermentation at a certain temperature after mixing, drying materials, cooling, broken mistake, vacuum are saved obtain Se-enriched feedstuff additive after fermentation.
Description
Technical field
It is specifically a kind of to be raised using Moringa waste wood preparation selenium-rich the invention belongs to feed addictive processing technique field
The method of feed additives.
Background technique
Moringa tree root has acid, so Moringa of gaining the name.Moringa is the perennial tropical leaf deciduous tree of Moringa, Moringaceae
Only one belongs to 14 kinds, and Moringa originates in north India Himalayas southern foot, is edible in Moringa and most study, most has
The kind of commercial value.Currently, Moringa is planted in Asia and African subtropical and tropical zones extensively.China is from 20th century 60
Plantation is carried out in recent years on Taiwan, Guangdong, Guangxi, Fujian, Yunnan and other places since age, on the south the plantation development to the Changjiang river of Moringa
Each provinces and regions.Moringa is drought-resistant, impoverishment tolerant, growth are rapid, adaptable, is the quick growing species of trees of subtropical and tropical zones.For many years
Come, Moringa is described as god by western scientist and grants the most precious present of the mankind, and this present is called " tree of miracle " by people.
Root, stem, leaf, flower, seed, branch and the bark of Moringa nutritional and officinal compositions rich in, are good raw-food materials
And animal and fowl fodder raw material.
Selenium is the essential trace elements of the human body, because it is with anti-oxidant, enhancing immunity of organisms, antagonism heavy metal, enhancing
The effects of reproductive function, the synthesis of regulatory protein matter and absorption of nutrient and be described as in body trace element by scientist
" king of anticancer ", human body selenium deficiency will cause a variety of diseases, such as Keshan disease, Kaschin-Beck disease, cancer, cardiovascular disease, cataract,
Selenium has important physiological function to human body, and the selenium in human body is mainly obtained from diet, and selenium contains in diet
Amount directly affects the intake of the daily selenium of people.The selenium element contained in food is very big by geographic influence, in soil selenium at
Divide and cause the huge difference of each department Se content, although some areas are containing selenium with the harmful substances such as mercury, unsuitable people
Body is absorbed and utilized, can be by greatly developing planting industry and aquaculture, from plant and animal in order to allow human body to obtain selenium element
Selenium element is obtained, selenium-rich livestock and fowls product is one of the important channel of human body Selenium Supplement.Livestock and poultry selenium-rich can pass through reasonable selenium-rich
Feed arrangement obtains, and the content of selenium in feed can be improved by the way that selenium-rich substance is added into feed, supplement selenium element to livestock and poultry.
The immune function of livestock and poultry body can be enhanced in Se-enriched feedstuff, enhances body to the resistance of disease, improves the fertility of livestock and poultry,
The meat Egg Quality that livestock and poultry can also enough be improved, improves the Se content of meat egg in livestock and poultry.
Chinese Patent Application No.: 201210169831.2, a kind of patent name: Moringa extract and preparation method thereof and peppery
The wooden feed addictive.The patent preparation method is that leaf of Moringa is added to water, is digested after adjusting pH value and temperature with biological enzyme, so
Heating extraction obtains Aqueous extracts, alcohol precipitation after Aqueous extracts concentration afterwards, and gained precipitating is Moringa extract, which is made
For feed addictive.
Chinese Patent Application No.: CN201710341572.X, a kind of patent name: preparation of selenium-rich Moringa feed addictive
Method and application thereof.The preparation method of the patent disclosure the following steps are included: (1) Moringa raw material acquisition and pretreatment；(2) peppery
Wood maturation process: by pretreated Moringa coarse powder temperature be 45~60 DEG C under conditions of be sealed by fermentation 6~8 days, then
It takes grain out of a granary to sun it broken 1 time, is finally sealed by fermentation 2~3 days under conditions of 35~50 DEG C, after obtaining the curing that rotten degree is 80~87%
Moringa powder；(3) drying and preservation of moringa powder.
Although preparing feed addictive now with fermentation process is carried out to Moringa tree branches and leaves in technology, actually using
In the process the nutritional ingredient in Moringa can not by raising livestock and poultry effective use, the meat egg product Se content produced compared with
It is low.
Summary of the invention
The present invention provides a kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood, and this method can obtain excellent
The Se-enriched feedstuff additive of matter can be effectively reduced harm of the alkaloid to raising livestock and poultry, the Se-enriched feedstuff additive in Moringa
Protein content is high, and full of nutrition, palatability is good, is easy to be raised livestock and poultry absorption, is conducive to the growth for raising livestock and poultry, obtains
Livestock meat egg product Se content is higher, and nutritive value is high, is conducive to absorption of human body utilization, while this method can effectively improve pair
Moringa leftover pieces are efficiently used, and are economized on resources.
The scheme of the invention is realize in this way: a kind of side preparing Se-enriched feedstuff additive using Moringa waste wood
Method, comprising the following steps:
(1) mechanical activation pre-processes: it is that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, it will be rich
The discarded wooden branch of selenium Moringa and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and mass ratio is added in the ball mill and is
Selenium-rich moringa powder, the high aluminium ball ball-milling medium, Chinese herbal medicine solution of 1:2:1.5, and selenium-rich moringa powder, high aluminium ball ball milling are situated between
80% that total volume is ball mill volume is added in matter and Chinese herbal medicine solution, then adjust drum's speed of rotation be 380 ~ 400r/min and
Temperature is taken out after carrying out 2 ~ 3h of activation for 60 ~ 80 DEG C, and feed liquid after treatment is separated with high aluminium ball ball-milling medium, is taken
Feed liquid after separation is evaporated in vacuo to obtain activation selenium-rich moringa powder；
(2) fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, be added activation selenium-rich moringa powder quality 5 ~
0.5 ~ 1% glucose that activation selenium-rich moringa powder quality is added, activation selenium-rich moringa powder quality is added in 6% composite bacteria
0.2 ~ 0.5% lactose, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.5 ~ 1:2.0, after mixing 27 ~ 36
It is sealed 3 ~ 4d of fermentation at DEG C, after fermentation dries material at 50 ~ 60 DEG C, carry out crushed 30 after cooling ~
40 meshes, vacuum, which saves, obtains Se-enriched feedstuff additive.
As a further improvement of the present invention, the Chinese herbal medicine fluid is the preparation method comprises the following steps: according to parts by weight than taking Schisandra chinensis 8
~ 10 parts, 10 ~ 12 parts of creeping oxalis, fork divide 12 ~ 15 parts of knotweed, 8 ~ 10 parts of Fructus Corni, 6 ~ 8 parts of Chinese herbaceous peony mixing after carry out being ground into 10 mesh
Mixed-powder, into mixed-powder be added volume fraction be 50% ethyl alcohol make solid-liquid ratio be 1:3, then in ultrasonic power
It is progress 10 ~ 12min of ultrasonic extraction at 30 ~ 40 DEG C for 180 ~ 200W and temperature, is then centrifuged under the conditions of 5000r/min
Supernatant is taken to obtain Chinese herbal medicine fluid.
As a further improvement of the present invention, the composite bacteria constituent and its weight fraction ratio are as follows: compound ferment
It is 15 ~ 20 parts of female bacterium, 8 ~ 10 parts of lactic acid bacteria, 6 ~ 8 parts of bacillus subtilis, 9 ~ 11 parts of bacillus licheniformis, 5 ~ 8 parts of aspergillus oryzae, black
6 ~ 10 parts of aspergillus, 8 ~ 10 parts of trichodermaharzianum.
As a further improvement of the present invention, the mulriple yeasts preparation method are as follows:
A. it chooses strain: choosing 8 ~ 10 parts of saccharomyces carlsbergensis bacterium, 10 ~ 12 parts of candida utili bacterium, the torrid zone according to weight fraction ratio
12 ~ 15 parts of Candida, 8 ~ 10 parts of brewer's yeast, 6 ~ 12 parts of rhodotorula；
B. induction mutation of bacterium: by 8 ~ 10 parts of saccharomyces carlsbergensis bacterium, 10 ~ 12 parts of candida utili bacterium, 12 ~ 15 parts of candida tropicalis,
Picking mixed bacteria is inoculated on PDA slant medium after mixing for 8 ~ 10 parts of brewer's yeast, 6 ~ 12 parts of rhodotorula, at 30 DEG C
48h is cultivated, with the saccharomycete spore for passing through slant activation under sterile washing, is added in the triangular flask for having bead and is shaken
It is filtered after 20min with four layers of lens paper, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, takes 4mL's
Yeast bacterium suspension carries out 120 ~ 150s of ultraviolet irradiation mutagenesis in the sterilized culture dish with magnetic stirring bar, then will be through
The yeast bacterium suspension for crossing ultraviolet mutagenesis processing uses DES dilution 25 ~ 30min of mutagenic treatment again, is then rapidly added
10ml25% sodium thiosulfate terminates reaction and obtains mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by c. Zengjing Granule, and malt leaching is added
Powder culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
D. the barms of overnight incubation are inoculated into solid medium with 1% amount, 30 DEG C of 48 ~ 72h of static gas wave refrigerator are selected
The yeast strain to grow fine obtains mulriple yeasts.
As a further improvement of the present invention, the PDA culture medium formula and its preparation condition are potato 200g, Portugal
Grape sugar 20g, agar 15g, water 1000mL, natural ph, 121 DEG C, 0.1MPa sterilizing 15min.
As a further improvement of the present invention, the fructus hordei germinatus leaching powder liquid culture medium formula and its preparation condition are glucose
10g, peptone 2g, fructus hordei germinatus leaching powder 1g, yeast extract 1g, agar powder 12g, lactic acid 0.3mL/L, water 1000mL, 121 DEG C of sterilizings
30min。
As a further improvement of the present invention, the solid culture based formulas and its preparation condition are 0.1mm granularity
Moringa waste wood powder 20g, peptone 1g, glucose 2g, NaCl10g, agar powder 20g, aquae destillata 1000mL, pH5.5 ~ 6.0,121
DEG C sterilizing 30min.
As a further improvement of the present invention, the Se-enriched feedstuff additive is in the raising of poultry, which to add
The additive amount for adding agent is 2 ~ 3%.
The technical principle that the present invention realizes is:
The present invention carries out processing to discarded Moringa branch using the integrated processes of mechanical activation and microbial fermentation and prepares Moringa selenium-rich
Feed addictive.Chinese herbal medicine fluid is added while carrying out mechanical activation, the Chinese herbal medicine fluid of addition is acid Chinese herbal medicine fluid, favorably
Proteolytic in Moringa wooden branch and leaf of Moringa is in acid Chinese herbal medicine fluid.Moringa branch and Moringa after mechanical activation
Fiber macromolecular chain in leaf is interrupted destructurized, the formation relatively small strand of the degree of polymerization, the net of Moringa cellulose
For shape structure by different degrees of destruction, fiber is loose, and unformed area increases, and specific surface area increases, and reactivity improves, and has
It preferably degrades to Moringa conducive to microorganism, to effectively reduce fermentation time, improves the efficiency of microbial fermentation.Pass through
The interaction of Chinese herbal medicine fluid and mechanical ball mill preferably extracts the nutriments such as the protein in Moringa wooden branch and leaf of Moringa
Out, can carry out enzymatic hydrolysis by the protease that microorganism generates in the step of protein being leached out is fermented below makes
It obtains in Se-enriched feedstuff additive containing the small molecular protein peptide for being more conducive to cultivation livestock and poultry absorption.In addition, in the Chinese herbal medicine fluid being added
Schisandra chinensis (Classification system: Schisandra chinensis), creeping oxalis (Classification system: Oxalis corniculata L.),
Fork divides knotweed (Classification system: Polygonum divaricatum L.), Fructus Corni (Latin literary fame: Cornifructus), Chinese herbaceous peony
(Classification system: Paeonia lactiflora Pall.) has antipyretic diuresis, eliminating stagnated blood to disperse swelling, sterilization and other effects, adjustable
The functions of intestines and stomach of livestock and poultry is cultivated, the immunity of cultivation livestock and poultry is improved.
Carrying out mutagenic treatment to the yeast saccharomyces strain selected can be improved the fermentation activity of composite yeast strain, adaptability
And stability, to promote degradation of the barms to selenium-rich Moringa, so as to improve the nutritive value of selenium-rich Moringa.By producing protein
Candida, lactic acid bacteria fermentation, the selenium in Moringa can be carried out processing become organic selenium, be conducive to cultivate livestock and poultry pair
The absorption and utilization of selenium, improve the conversion ratio of selenium, and the meat egg product obtained from is easier to be absorbed by the body, and are that a kind of safety has
The method of effect.Hay bacillus, brewer's yeast can generate a variety of digestive ferments such as protease, lipase and B family vitamin, improve rich
The utilization rate of selenium forage additive.The phase of the strains such as barms, bacillus subtilis, bacillus licheniformis during the fermentation
Interaction can degrade to the alkaloid contained in Moringa, to reduce harm of the alkaloid to cultivation livestock and poultry, improve rich
The safety of selenium forage additive.Aspergillus niger and candida utili can be degraded the tannin in Moringa by fermentation,
The content of tannin in Se-enriched feedstuff additive is reduced, to eliminate influence of the tannin to Se-enriched feedstuff additive quality, is improved rich
The palatability of selenium forage additive improves the feed intake of cultivation livestock and poultry.Pass through the complementary symbiosis of the microorganism of addition, interaction
Mycoprotein to make the low quality protein in selenium-rich Moringa be converted to high quality can be improved in Se-enriched feedstuff additive
Protein content, also reduce coarse-fibred content, while a large amount of mycoproteins by accumulating in fermentation process
And metabolite, the nutrient content of Se-enriched feedstuff additive can be effectively improved.Glucose and lactose are added during the fermentation
The growth that can promote fermenting microbe promotes the progress of fermentation.
The present invention has following good result:
1. it can be effectively reduced the content of poisonous alkaloids in Moringa after method of the invention handles Moringa waste wood, thus
Harm of the poisonous alkaloids to cultivation livestock and poultry is reduced, cultivation livestock and poultry poisoning is avoided.
2. the present invention, which handles discarded Moringa branch, can reduce Moringa crude fiber content, the egg of the Se-enriched feedstuff additive is improved
White matter content effectively facilitates the Se-enriched feedstuff additive and is absorbed and utilized by cultivation livestock and poultry, and the Se-enriched feedstuff that the present invention obtains adds
Add agent that can provide sufficient nutriment in the case where reducing dosage for cultivation livestock and poultry, effectively facilitates the growth of cultivation livestock and poultry
And breeding, the good selenium-rich meat egg product of output, application value are high.
3. preparation method of the invention is 71.6 ~ 72.9% for the Moringa Se-enriched feedstuff additive crude protein content of production,
Crude fiber content is 10.6 ~ 11.3%, and crude fat content is 5.5 ~ 5.9%, and alkaloid is 0.01 ~ 0.02%；It will be of the invention
The Moringa Se-enriched feedstuff additive application that method is prepared can effectively improve chicken meat, soil in the cultivation of chicken and aardvark
The content of selenium in pork, wherein the content of selenium is 0.276 ~ 0.314mg.kg in chicken meat-1, the content of selenium is in aardvark meat
0.342~0.418 mg.kg-1, the Moringa Se-enriched feedstuff additive that method of the invention obtains can significantly improve chicken and pork
The content of middle selenium.
Specific embodiment
A kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood of the description present invention with reference to embodiments, this
A little descriptions are not to be further limited to the content of present invention.
PDA culture medium formula and its preparation condition are as follows: potato 200g, glucose 20g, agar 15g, water 1000mL,
Natural ph, 121 DEG C, 0.1MPa sterilizing 15min.
Fructus hordei germinatus leaching powder liquid culture medium formula and its preparation condition are as follows: glucose 10g, peptone 2g, fructus hordei germinatus leaching powder 1g, ferment
Female cream 1g, agar powder 12g, lactic acid 0.3mL/L, water 1000mL, 121 DEG C of sterilizing 30min.
Solid culture based formulas and its preparation condition are as follows: for Moringa waste wood powder 20g, the peptone 1g, Portugal of 0.1mm granularity
1000mL, pH5.5 ~ 6.0,121 DEG C of grape sugar 2g, NaCl10g, agar powder 20g, aquae destillata sterilizing 30min.
Embodiment 1
Chinese herbal medicine fluid preparation: according to parts by weight than taking 8.5 parts of Schisandra chinensis, 12 parts of creeping oxalis, fork to divide 13.5 parts of knotweed, Fructus Corni
9.5 parts, 8 parts of Chinese herbaceous peony mixing after carry out the mixed-powder for being ground into 10 mesh, into mixed-powder be added volume fraction be 50% second
Alcohol makes solid-liquid ratio be 1:3, and ultrasonic extraction 11min is then carried out in the case where ultrasonic power is 180W and temperature is 40 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 9 parts of saccharomyces carlsbergensis bacterium, 11.5 parts of candida utili bacterium, the false silk ferment in the torrid zone according to weight fraction ratio
Female 15 parts, 9.5 parts of brewer's yeast, 12 parts of rhodotorula；
Induction mutation of bacterium: by 9 parts of saccharomyces carlsbergensis bacterium, 11.5 parts of candida utili bacterium, 15 parts of candida tropicalis, brewer's yeast
9.5 parts, picking mixed bacteria is inoculated on PDA slant medium 12 parts of rhodotorula after mixing, in 30 DEG C of culture 48h, use
Pass through the saccharomycete spore of slant activation under sterile washing, is added in the triangular flask for having bead after carrying out concussion 20min with four
Layer lens paper filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, and the saccharomycete of 4mL is taken to suspend
Liquid carries out ultraviolet irradiation mutagenesis 130s in the sterilized culture dish with magnetic stirring bar, then will be by ultraviolet mutagenesis
The yeast bacterium suspension of reason uses DES dilution mutagenic treatment 27min again, is then rapidly added the termination of 10ml25% sodium thiosulfate
Reaction obtains mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 65h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 385r/min and temperature is 70
It is taken out after DEG C carrying out activation 3h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the feed liquid after taking separation
It is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 5.5% of activation selenium-rich moringa powder quality is added
15 parts of mulriple yeasts, 9.5 parts of lactic acid bacteria, 6.5 parts of bacillus subtilis, 10 parts of bacillus licheniformis, 6 parts of aspergillus oryzae, black
7 parts of aspergillus, 0.8% glucose that activation selenium-rich moringa powder quality is added, is added and lives 8 parts of the trichodermaharzianum composite bacterias formed
0.5% lactose for changing selenium-rich moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.5, is uniformly mixed
Fermentation 4d is sealed at 30 DEG C afterwards, after fermentation dries material at 52 DEG C, carries out crushed 40 after cooling
Mesh, vacuum, which saves, obtains Se-enriched feedstuff additive.
Embodiment 2
Chinese herbal medicine fluid preparation: according to parts by weight than take 9 parts of Schisandra chinensis, 11 parts of creeping oxalis, fork divide 12 parts of knotweed, 10 parts of Fructus Corni,
The mixed-powder for being ground into 10 mesh is carried out after 7.5 parts of Chinese herbaceous peony mixing, the ethyl alcohol that volume fraction is 50% is added into mixed-powder to be made
Obtaining solid-liquid ratio is 1:3, and ultrasonic extraction 10.5min is then carried out in the case where ultrasonic power is 195W and temperature is 32 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 8 parts of saccharomyces carlsbergensis bacterium, 12 parts of candida utili bacterium, candida tropicalis according to weight fraction ratio
13 parts, 9 parts of brewer's yeast, 8 parts of rhodotorula；
Induction mutation of bacterium: by 8 parts of saccharomyces carlsbergensis bacterium, 12 parts of candida utili bacterium, 13 parts of candida tropicalis, 9 parts of brewer's yeast,
Picking mixed bacteria is inoculated on PDA slant medium 8 parts of rhodotorula after mixing, in 30 DEG C of culture 48h, with sterile washing
The lower saccharomycete spore by slant activation is added in the triangular flask for having bead after carrying out concussion 20min with four layers of lens paper
Filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, takes the yeast bacterium suspension of 4mL in sterilized
Cingula, which has, carries out ultraviolet irradiation mutagenesis 120s in the culture dish of magnetic stirring bar, then by the yeast by ultraviolet mutagenesis processing
Bacterium suspension uses DES dilution mutagenic treatment 30min again, is then rapidly added the termination reaction of 10ml25% sodium thiosulfate and obtains
Mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 48h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 390r/min and temperature is 60
It is taken out after DEG C carrying out activation 2h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the feed liquid after taking separation
It is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 5.8% of activation selenium-rich moringa powder quality is added
20 parts of mulriple yeasts, 8 parts of lactic acid bacteria, 8 parts of bacillus subtilis, 9 parts of bacillus licheniformis, 8 parts of aspergillus oryzae, aspergillus niger 6
Part, 8.5 parts of the trichodermaharzianum composite bacterias formed, 0.5% glucose that activation selenium-rich moringa powder quality is added, addition activate
0.4% lactose of selenium-rich moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.7, after mixing
It is sealed fermentation 3d at 36 DEG C, after fermentation dries material at 60 DEG C, carries out crushed 45 mesh after cooling
Sieve, vacuum, which saves, obtains Se-enriched feedstuff additive.
Embodiment 3
Chinese herbal medicine fluid preparation: according to parts by weight than taking 10 parts of Schisandra chinensis, 10.5 parts of creeping oxalis, fork to divide 13 parts of knotweed, Fructus Corni 9
The mixed-powder for being ground into 10 mesh is carried out after part, 6 parts of Chinese herbaceous peony mixing, the ethyl alcohol that volume fraction is 50% is added into mixed-powder
So that solid-liquid ratio is 1:3, ultrasonic extraction 11.5min is then carried out in the case where ultrasonic power is 200W and temperature is 30 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 9.5 parts of saccharomyces carlsbergensis bacterium, 11 parts of candida utili bacterium, the false silk ferment in the torrid zone according to weight fraction ratio
Female 12 parts, 8.5 parts of brewer's yeast, 10 parts of rhodotorula；
Induction mutation of bacterium: by 9.5 parts of saccharomyces carlsbergensis bacterium, 11 parts of candida utili bacterium, 12 parts of candida tropicalis, brewer's yeast
8.5 parts, picking mixed bacteria is inoculated on PDA slant medium 10 parts of rhodotorula after mixing, in 30 DEG C of culture 48h, use
Pass through the saccharomycete spore of slant activation under sterile washing, is added in the triangular flask for having bead after carrying out concussion 20min with four
Layer lens paper filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, and the saccharomycete of 4mL is taken to suspend
Liquid carries out ultraviolet irradiation mutagenesis 140s in the sterilized culture dish with magnetic stirring bar, then will be by ultraviolet mutagenesis
The yeast bacterium suspension of reason uses DES dilution mutagenic treatment 26min again, is then rapidly added the termination of 10ml25% sodium thiosulfate
Reaction obtains mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 54h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 380r/min and temperature is 75
It is taken out after DEG C carrying out activation 2.8h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the material after taking separation
Liquid is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 5.2% of activation selenium-rich moringa powder quality is added
16 parts of mulriple yeasts, 10 parts of lactic acid bacteria, 7 parts of bacillus subtilis, 10.5 parts of bacillus licheniformis, 7 parts of aspergillus oryzae, black song
The composite bacteria of mould 8 parts, 10 parts of trichodermaharzianum compositions, 0.6% glucose that activation selenium-rich moringa powder quality is added are added and live
0.3% lactose for changing selenium-rich moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:2.0, is uniformly mixed
Fermentation 4d is sealed at 34 DEG C afterwards, after fermentation dries material at 50 DEG C, carries out crushed 30 after cooling
Mesh, vacuum, which saves, obtains Se-enriched feedstuff additive.
Embodiment 4
Chinese herbal medicine fluid preparation: according to parts by weight than take 9.5 parts of Schisandra chinensis, 10 parts of creeping oxalis, fork divide 14 parts of knotweed, 8 parts of Fructus Corni,
The mixed-powder for being ground into 10 mesh is carried out after 6.5 parts of Chinese herbaceous peony mixing, the ethyl alcohol that volume fraction is 50% is added into mixed-powder to be made
Obtaining solid-liquid ratio is 1:3, and ultrasonic extraction 10min is then carried out in the case where ultrasonic power is 185W and temperature is 35 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 10 parts of saccharomyces carlsbergensis bacterium, 10.5 parts of candida utili bacterium, the false silk ferment in the torrid zone according to weight fraction ratio
Female 14 parts, 8 parts of brewer's yeast, 6 parts of rhodotorula；
Induction mutation of bacterium: by 10 parts of saccharomyces carlsbergensis bacterium, 10.5 parts of candida utili bacterium, 14 parts of candida tropicalis, brewer's yeast 8
Part, picking mixed bacteria is inoculated on PDA slant medium 6 parts of rhodotorula after mixing, and in 30 DEG C of culture 48h, use is sterile
The lower saccharomycete spore Jing Guo slant activation of washing, is added in the triangular flask for having bead after carrying out concussion 20min with four layers of mirror
Head paper filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, take the yeast bacterium suspension of 4mL in
Ultraviolet irradiation mutagenesis 135s is carried out in the sterilized culture dish with magnetic stirring bar, it then will be by ultraviolet mutagenesis processing
Yeast bacterium suspension uses DES dilution mutagenic treatment 25min again, is then rapidly added 10ml25% sodium thiosulfate and terminates reaction
Obtain mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 72h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 400r/min and temperature is 80
It is taken out after DEG C carrying out activation 2.5h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the material after taking separation
Liquid is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 5% of activation selenium-rich moringa powder quality is added
17 parts of mulriple yeasts, 9 parts of lactic acid bacteria, 7.5 parts of bacillus subtilis, 11 parts of bacillus licheniformis, 5 parts of aspergillus oryzae, aspergillus niger
1% glucose that activation selenium-rich moringa powder quality is added, activation is added in the composite bacteria of 10 parts, 9.5 parts of trichodermaharzianum compositions
0.2% lactose of selenium-rich moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.6, after mixing
It is sealed fermentation 3.5d at 27 DEG C, after fermentation dries material at 58 DEG C, carries out crushed 42 after cooling
Mesh, vacuum, which saves, obtains Se-enriched feedstuff additive.
Embodiment 5
Chinese herbal medicine fluid preparation: according to parts by weight than taking 8 parts of Schisandra chinensis, 11.5 parts of creeping oxalis, fork to divide 15 parts of knotweed, Fructus Corni 8.5
The mixed-powder for being ground into 10 mesh is carried out after part, 7 parts of Chinese herbaceous peony mixing, the ethyl alcohol that volume fraction is 50% is added into mixed-powder
So that solid-liquid ratio is 1:3, ultrasonic extraction 12min is then carried out in the case where ultrasonic power is 190W and temperature is 38 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 8.5 parts of saccharomyces carlsbergensis bacterium, 10 parts of candida utili bacterium, the false silk ferment in the torrid zone according to weight fraction ratio
Female 13 parts, 10 parts of brewer's yeast, 9 parts of rhodotorula；
Induction mutation of bacterium: by 8.5 parts of saccharomyces carlsbergensis bacterium, 10 parts of candida utili bacterium, 13 parts of candida tropicalis, brewer's yeast 10
Part, picking mixed bacteria is inoculated on PDA slant medium 9 parts of rhodotorula after mixing, and in 30 DEG C of culture 48h, use is sterile
The lower saccharomycete spore Jing Guo slant activation of washing, is added in the triangular flask for having bead after carrying out concussion 20min with four layers of mirror
Head paper filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, take the yeast bacterium suspension of 4mL in
Ultraviolet irradiation mutagenesis 150s is carried out in the sterilized culture dish with magnetic stirring bar, it then will be by ultraviolet mutagenesis processing
Yeast bacterium suspension uses DES dilution mutagenic treatment 28min again, is then rapidly added 10ml25% sodium thiosulfate and terminates reaction
Obtain mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 60h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 390r/min and temperature is 65
It is taken out after DEG C carrying out activation 2.2h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the material after taking separation
Liquid is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 6% of activation selenium-rich moringa powder quality is added
18 parts of mulriple yeasts, 8.5 parts of lactic acid bacteria, 6 parts of bacillus subtilis, 9.5 parts of bacillus licheniformis, 6 parts of aspergillus oryzae, aspergillus niger
The composite bacteria of 9 parts, 9 parts of trichodermaharzianum compositions, 0.7% glucose that activation selenium-rich moringa powder quality is added, addition activation are rich
0.5% lactose of selenium moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.8, is existed after mixing
It is sealed fermentation 3d at 32 DEG C, after fermentation dries material at 55 DEG C, carries out crushed 48 meshes after cooling,
Vacuum, which saves, obtains Se-enriched feedstuff additive.
In order to verify the quality for the Se-enriched feedstuff additive that the present invention is prepared, the embodiment of the present invention 1 ~ 5 is prepared into
The Se-enriched feedstuff additive arrived carries out constituent analysis, while the Se-enriched feedstuff additive that the bright embodiment 1 ~ 5 of this law is prepared
It is fed for chicken and aardvark, Se-enriched feedstuff additive amount is the 2% of feed, and selenium in chicken and pork is tested after feeding 120 days
Content, commercially available similar Se-enriched feedstuff additive is subjected to same analysis test as a control group, analyzes situation such as following table institute
Show.
1 Se-enriched feedstuff additive component of table analyzes situation
The content situation of 2 chicken of table, aardvark meat selenium
The above embodiment of the present invention scheme is only the description of the invention and cannot limit the present invention, this is indicated in claim
Invention product form ingredient, component ratio, the range of preparation method parameter, and above-mentioned explanation does not point out parameter of the present invention
Range, therefore, any change within the meaning and scope equivalent to the claims of the present invention, be all considered to be including
Within the scope of the claims.
The present invention is created by multidigit feed processing technology personnel's long-term work experience accumulation, and by creative work
And go out, Se-enriched feedstuff additive Se content of the present invention is high, and protein content is high, and full of nutrition, palatability is high, is conducive to raising poultry
Fowl is absorbed and utilized, and enhances the quality of meat egg product, Se-enriched feedstuff application value with higher.
Claims (7)
1. a kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood, characterized in that the following steps are included:
The pretreatment of step 1) mechanical activation: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, will
The discarded wooden branch of selenium-rich Moringa and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and mass ratio is added in the ball mill
Selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution for 1:2:1.5, and make selenium-rich moringa powder, high aluminium ball ball milling
80% that total volume is ball mill volume is added in medium and Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 380 ~ 400r/min
It is taken out after carrying out 2 ~ 3h of activation with temperature for 60 ~ 80 DEG C, feed liquid after treatment is separated with high aluminium ball ball-milling medium,
Feed liquid after taking separation is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Step 2) fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and activation selenium-rich moringa powder quality is added
5 ~ 6% composite bacteria, be added activation selenium-rich moringa powder quality 0.5 ~ 1% glucose, be added activation selenium-rich Moringa silty
0.2 ~ 0.5% lactose of amount, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.5 ~ 1:2.0, is existed after mixing
It is sealed 3 ~ 4d of fermentation at 27 ~ 36 DEG C, after fermentation dries material at 50 ~ 60 DEG C, is crushed after cooling
30 ~ 40 meshes are crossed, vacuum, which saves, obtains Se-enriched feedstuff additive；
The composite bacteria constituent and its weight fraction ratio are as follows: 15 ~ 20 parts of mulriple yeasts, 8 ~ 10 parts of lactic acid bacteria, withered
6 ~ 8 parts of careless bacillus, 9 ~ 11 parts of bacillus licheniformis, 5 ~ 8 parts of aspergillus oryzae, 6 ~ 10 parts of aspergillus niger, 8 ~ 10 parts of trichodermaharzianum.
2. a kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood according to claim 1, characterized in that institute
Chinese herbal medicine fluid is stated the preparation method comprises the following steps: according to parts by weight than taking 8 ~ 10 parts of Schisandra chinensis, 10 ~ 12 parts of creeping oxalis, fork to divide knotweed 12 ~ 15
The mixed-powder for being ground into 10 mesh is carried out after part, 8 ~ 10 parts of Fructus Corni, 6 ~ 8 parts of Chinese herbaceous peony mixing, volume is added into mixed-powder
The ethyl alcohol that score is 50% makes solid-liquid ratio be 1:3, then carries out in the case where ultrasonic power is 180 ~ 200W and temperature is 30 ~ 40 DEG C
Then 10 ~ 12min of ultrasonic extraction carries out centrifuging and taking supernatant under the conditions of 5000r/min and obtains Chinese herbal medicine fluid.
3. a kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood according to claim 1, characterized in that institute
The mulriple yeasts preparation method stated are as follows:
A. it chooses strain: choosing 8 ~ 10 parts of saccharomyces carlsbergensis bacterium, 10 ~ 12 parts of candida utili bacterium, the torrid zone according to weight fraction ratio
12 ~ 15 parts of Candida, 8 ~ 10 parts of brewer's yeast, 6 ~ 12 parts of rhodotorula；
B. induction mutation of bacterium: by 8 ~ 10 parts of saccharomyces carlsbergensis bacterium, 10 ~ 12 parts of candida utili bacterium, 12 ~ 15 parts of candida tropicalis,
Picking mixed bacteria is inoculated on PDA slant medium after mixing for 8 ~ 10 parts of brewer's yeast, 6 ~ 12 parts of rhodotorula, at 30 DEG C
48h is cultivated, with the saccharomycete spore for passing through slant activation under sterile washing, is added in the triangular flask for having bead and is shaken
It is filtered after 20min with four layers of lens paper, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, takes 4mL's
Yeast bacterium suspension carries out 120 ~ 150s of ultraviolet irradiation mutagenesis in the sterilized culture dish with magnetic stirring bar, then will be through
The yeast bacterium suspension for crossing ultraviolet mutagenesis processing uses DES dilution 25 ~ 30min of mutagenic treatment again, is then rapidly added
10ml25% sodium thiosulfate terminates reaction and obtains mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by c. Zengjing Granule, and malt leaching is added
Powder culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
D. the barms of overnight incubation are inoculated into solid medium with 1% amount, 30 DEG C of 48 ~ 72h of static gas wave refrigerator are selected
The yeast strain to grow fine obtains mulriple yeasts.
4. a kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood according to claim 2, characterized in that institute
The PDA culture medium formula and its preparation condition stated are potato 200g, glucose 20g, agar 15g, water 1000mL, natural pH
Value, 121 DEG C, 0.1MPa sterilizing 15min.
5. a kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood according to claim 2, characterized in that institute
The fructus hordei germinatus leaching powder liquid culture medium formula and its preparation condition stated be glucose 10g, peptone 2g, fructus hordei germinatus leaching powder 1g, yeast extract 1g,
Agar powder 12g, lactic acid 0.3mL/L, water 1000mL, 121 DEG C of sterilizing 30min.
6. a kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood according to claim 2, characterized in that institute
The solid culture based formulas and its preparation condition stated be the Moringa waste wood powder 20g of 0.1mm granularity, peptone 1g, glucose 2g,
1000mL, pH5.5 ~ 6.0,121 DEG C of NaCl10g, agar powder 20g, aquae destillata sterilizing 30min.
7. a kind of method for preparing Se-enriched feedstuff additive using Moringa waste wood according to claim 1, characterized in that
The Se-enriched feedstuff additive is in the raising of poultry, the additive amount of the Se-enriched feedstuff additive to be 2 ~ 3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811234966.6A CN109362948A (en) | 2018-10-23 | 2018-10-23 | A method of Se-enriched feedstuff additive is prepared using Moringa waste wood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811234966.6A CN109362948A (en) | 2018-10-23 | 2018-10-23 | A method of Se-enriched feedstuff additive is prepared using Moringa waste wood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109362948A true CN109362948A (en) | 2019-02-22 |
Family
ID=65400695
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811234966.6A Withdrawn CN109362948A (en) | 2018-10-23 | 2018-10-23 | A method of Se-enriched feedstuff additive is prepared using Moringa waste wood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109362948A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111406834A (en) * | 2020-05-26 | 2020-07-14 | 深圳市东荣生物科技有限责任公司 | Microbial agent for promoting growth of poultry and livestock |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102783574A (en) * | 2012-07-30 | 2012-11-21 | 嘉兴市壹零生物科技有限公司 | Method for replacing corn ingredients in pig feed |
| CN102870966A (en) * | 2012-06-24 | 2013-01-16 | 邵素英 | Feed additive product with high efficiency |
| CN103181464A (en) * | 2011-12-28 | 2013-07-03 | 宋建生 | Sheep microecological feed additive and preparation method thereof |
| CN105394397A (en) * | 2015-12-03 | 2016-03-16 | 安徽五粮泰生物工程股份有限公司 | Preparation method of suckling pig yogurt feed |
| CN106107130A (en) * | 2016-07-19 | 2016-11-16 | 合肥莱斯生物工程有限公司 | A kind of pig feed additive being beneficial to intestinal relieving constipation and preparation method thereof |
| CN106721021A (en) * | 2016-12-30 | 2017-05-31 | 长春禾丰饲料有限责任公司 | A kind of piglet antibiotic-free feed and preparation method thereof |
| CN106962594A (en) * | 2017-04-26 | 2017-07-21 | 中国科学院合肥物质科学研究院 | A kind of Selenium-enriched fermentation dregs of beans, preparation method and application |
| CN107006692A (en) * | 2017-05-16 | 2017-08-04 | 广西壮族自治区农业科学院农业资源与环境研究所 | A kind of preparation method and its usage of selenium-rich Moringa feed addictive |
| CN107201315A (en) * | 2016-12-30 | 2017-09-26 | 徐州工程学院 | A kind of Paecilomyces thermaphila mutagenic strain and its method of mutagenesis and application |
| CN107868778A (en) * | 2017-11-07 | 2018-04-03 | 常州禾吉纺织品有限公司 | A kind of preparation method of Se-enriched yeast |
-
2018
- 2018-10-23 CN CN201811234966.6A patent/CN109362948A/en not_active Withdrawn
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181464A (en) * | 2011-12-28 | 2013-07-03 | 宋建生 | Sheep microecological feed additive and preparation method thereof |
| CN102870966A (en) * | 2012-06-24 | 2013-01-16 | 邵素英 | Feed additive product with high efficiency |
| CN102783574A (en) * | 2012-07-30 | 2012-11-21 | 嘉兴市壹零生物科技有限公司 | Method for replacing corn ingredients in pig feed |
| CN105394397A (en) * | 2015-12-03 | 2016-03-16 | 安徽五粮泰生物工程股份有限公司 | Preparation method of suckling pig yogurt feed |
| CN106107130A (en) * | 2016-07-19 | 2016-11-16 | 合肥莱斯生物工程有限公司 | A kind of pig feed additive being beneficial to intestinal relieving constipation and preparation method thereof |
| CN106721021A (en) * | 2016-12-30 | 2017-05-31 | 长春禾丰饲料有限责任公司 | A kind of piglet antibiotic-free feed and preparation method thereof |
| CN107201315A (en) * | 2016-12-30 | 2017-09-26 | 徐州工程学院 | A kind of Paecilomyces thermaphila mutagenic strain and its method of mutagenesis and application |
| CN106962594A (en) * | 2017-04-26 | 2017-07-21 | 中国科学院合肥物质科学研究院 | A kind of Selenium-enriched fermentation dregs of beans, preparation method and application |
| CN107006692A (en) * | 2017-05-16 | 2017-08-04 | 广西壮族自治区农业科学院农业资源与环境研究所 | A kind of preparation method and its usage of selenium-rich Moringa feed addictive |
| CN107868778A (en) * | 2017-11-07 | 2018-04-03 | 常州禾吉纺织品有限公司 | A kind of preparation method of Se-enriched yeast |
Non-Patent Citations (1)
| Title |
|---|
| 何培新: "《高级微生物学》", 31 August 2017, 中国轻工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111406834A (en) * | 2020-05-26 | 2020-07-14 | 深圳市东荣生物科技有限责任公司 | Microbial agent for promoting growth of poultry and livestock |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104473145B (en) | A kind of Antrodia camphorata submerged fermentation compound product and preparation method thereof | |
| CN102823726B (en) | It is a kind of improve cottonseed meal content and can detoxification biofermentation method | |
| CN101743854B (en) | Method for preparing morchella mycelium by using dendrobium fiber as substrate | |
| CN102987140A (en) | Method for preparing novel ruminant feed by using beneficial bacteria and plant fiber | |
| CN110214626B (en) | Straw mushroom culture method | |
| CN106343410A (en) | Production method of kelp extract | |
| CN101120719B (en) | Microorganism ferment purple sweet potato and preparation method thereof | |
| CN109362983A (en) | A kind of selenium-rich chicken feed and preparation method thereof | |
| CN101665373A (en) | Safe and high-efficiency preparation method of master culture medium material of edible fungi | |
| CN105053602A (en) | Mulberry pomace chicken feed and preparation method thereof | |
| CN109845899A (en) | A kind of sugarcane end pin white wine residue protein feed and preparation method thereof | |
| CN104472869A (en) | Mushroom bran protein biological feed additive and preparation method thereof | |
| CN106036320A (en) | Method for preparing mushroom mycelial fermentation broth original drink | |
| CN105647756A (en) | Selenium-enriched health care thick wine containing cordyceps sinensis and mulberries | |
| CN107080246A (en) | A kind of plant enzyme and preparation method thereof | |
| CN110616122A (en) | Cardamine hupingshanensis selenium-rich yellow wine and production method thereof | |
| CN109362956A (en) | A kind of selenium-rich ox feed and preparation method thereof | |
| CN109362948A (en) | A method of Se-enriched feedstuff additive is prepared using Moringa waste wood | |
| CN104509757A (en) | Processing method of cassava powder | |
| CN105935025A (en) | Cultivation material utilizing paper mulberry to cultivate domestic fungus and cultivation method thereof | |
| CN105192311A (en) | Livestock and poultry compound feed additive | |
| CN107568159A (en) | A kind of cultural method for promoting Shelled Turtle Trionyx Sinensis growth | |
| CN109006178A (en) | A kind of agaric culture medium and preparation method thereof | |
| CN107522527A (en) | A kind of cultivation matrix of prominent mushroom antivirus action and preparation method thereof | |
| CN114190489A (en) | Preparation method of microbial fermentation chicken feed based on mixture of hybrid broussonetia papyrifera and wormwood |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190222 |