CN109362956A - A kind of selenium-rich ox feed and preparation method thereof - Google Patents
A kind of selenium-rich ox feed and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to feed processing technology, specifically a kind of selenium-rich ox feed and preparation method thereof.Each constituent of selenium-rich ox feedstuff and its weight fraction ratio are as follows: 18 ~ 20 parts of corn flour, 16 ~ 18 parts of wheat flour, 12 ~ 15 parts of wheat bran, 10 ~ 13 parts of sweet potato vine, 7 ~ 9 parts of straw, 8 ~ 9 parts of grass meal, 5 ~ 7 parts of rapeseed dregs, 8 ~ 10 parts of potato starch, 3 ~ 4 parts of molasses, 3 ~ 5 parts of sodium bisulfate, 2 ~ 4.5 parts of Se-enriched feedstuff additive, 10 ~ 12 parts of carboxymethyl cellulose, 1 ~ 2 part of salt, 0.5 ~ 1 part of citric acid, 1 ~ 2 part of medical stone, 2 ~ 4 parts of preservative.Se-enriched feedstuff additive is first prepared using the method for mechanical lapping and fermentation during preparing Se-enriched feedstuff; then by raw material sweet potato vine and straw carry out being ground into mixed powder, finally mixed powder, Se-enriched feedstuff additive and remaining raw material are sent into pelletizer and is granulated and obtains selenium-rich ox feed.
Description
Technical field
The invention belongs to feed processing technology, specifically a kind of selenium-rich ox feed and preparation method thereof.
Background technique
Selenium is human body and the necessary microelement of animal, has important role to the growth and development of human body and animal, moreover it is possible to
The immunity for enough improving humans and animals body, improves the health of human body and animal.But in soil selenium distribution and it is uneven, have richness
The area of selenium and the area of selenium deficiency cause China to have the daily selenium amount of taking the photograph of the population in 2/3 area insufficient, although along with some areas
Containing selenium but with harmful substances such as mercury, is not suitable for absorption of human body and utilizes.Selenium is not only the necessary microelement of the mankind, and dynamic
The necessary microelement of object.In order to allow human body to obtain selenium element, can by greatly developing planting industry and aquaculture, from plant and
Selenium element is obtained in animal, selenium-rich livestock and fowls product is one of the important channel of human body Selenium Supplement.Livestock and poultry selenium-rich can be by reasonable
Se-enriched feedstuff arrange in pairs or groups to obtain, the content of selenium in feed can be improved by the way that selenium-rich substance is added into feed, give livestock and poultry supplement
Selenium element.The immune function of livestock and poultry body can be enhanced in Se-enriched feedstuff, enhances body to the resistance of disease, improves the numerous of livestock and poultry
Ability is grown, can also enough improve the meat Egg Quality of livestock and poultry, improve the Se content of meat egg in livestock and poultry.
It is well known that only mending certain its normal physiological function of selenium competence exertion in livestock and poultry diet.Currently, to livestock and poultry into
The method of row selenium-supply is to add the sodium stannite complexing agents of inorganic states in animal and fowl fodder to meet its needs, using inorganic selenium into
Row feeds and is not easy to absorb animal, while sodium selenite has certain toxicity, excessively replenishes and because its toxic effect is unfavorable for food
Safety.Organic selenium additive, including methionine chelate selenium, organic selenate, Se-enriched yeast, selenium-rich algae, selenium malt etc., poison
Property is more much lower than inorganic selenium.The most important advantage of organic selenium is that effective Se content is high, easily absorbs animal.
Beef cattle is to ensure that I crosses the important link of agricultural production system benign cycle, and promote urban and rural residents of China carnivorous
The important industry that consumption structure improves.Protein rich in, lean and containing needed by human body multi mineral in beef
Matter and amino acid, along with the raising of Living consumption and the variation of diet structure, the beef items market demand is vigorous.It will
Additive rich in selenium, which is added in the edible feed of beef cattle, can be such that Se content in beef cattle increases, and people are edible to contain energy after selenium-rich beef
Enough nutriments that nutriment in beef is changed into needed by human body, while improving Se content in human body.
Inorganic selenium is changed into organic selenium in existing Se-enriched feedstuff, but the nutrition in feed in actual use
Ingredient can not be efficiently used by ox, and beef, the dairy product Se content produced is lower, not be able to satisfy the daily of people
Demand.
Summary of the invention
The present invention provides a kind of selenium-rich ox feed and preparation method thereof, and the feed being prepared contains good selenium, and should
Dietary protein level is high, and nutrition is balanced, and palatability is good, is easy to be absorbed by livestock and poultry ox, is conducive to the growth of rear cattle, obtains
Beef and milk Se content are higher, be conducive to absorption of human body utilization, while this method can effectively improve to Moringa leftover pieces into
Row effective use, economizes on resources.
The scheme of the invention is realize in this way: a kind of selenium-rich ox feed, the selenium-rich ox feedstuff are respectively formed into
Point and its weight fraction ratio are as follows: 18 ~ 20 parts of corn flour, 16 ~ 18 parts of wheat flour, 12 ~ 15 parts of wheat bran, 10 ~ 13 parts of sweet potato vine, straw
7 ~ 9 parts, 8 ~ 9 parts of grass meal, 5 ~ 7 parts of rapeseed dregs, 8 ~ 10 parts of potato starch, 3 ~ 4 parts of molasses, 3 ~ 5 parts of sodium bisulfate, Se-enriched feedstuff
2 ~ 4.5 parts of additive, 10 ~ 12 parts of carboxymethyl cellulose, 1 ~ 2 part of salt, 0.5 ~ 1 part of citric acid, 1 ~ 2 part of medical stone, preservative 2
~ 4 parts.
As a further improvement of the present invention, the Se-enriched feedstuff addictive preparation method are as follows:
(1) mechanical activation pre-processes: it is that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, it will be rich
The discarded wooden branch of selenium Moringa and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and mass ratio is added in the ball mill and is
Selenium-rich moringa powder, the high aluminium ball ball-milling medium, Chinese herbal medicine solution of 1:2:1.5, and selenium-rich moringa powder, high aluminium ball ball milling are situated between
80% that total volume is ball mill volume is added in matter and Chinese herbal medicine solution, then adjust drum's speed of rotation be 380 ~ 400r/min and
Temperature is taken out after carrying out 2 ~ 3h of activation for 60 ~ 80 DEG C, and feed liquid after treatment is separated with high aluminium ball ball-milling medium, is taken
Feed liquid after separation is evaporated in vacuo to obtain activation selenium-rich moringa powder；
(2) fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, be added activation selenium-rich moringa powder quality 5 ~
0.5 ~ 1% glucose that activation selenium-rich moringa powder quality is added, activation selenium-rich moringa powder quality is added in 6% composite bacteria
0.2 ~ 0.5% lactose, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.5 ~ 1:2.0, after mixing 27 ~ 36
It is sealed 3 ~ 4d of fermentation at DEG C, after fermentation dries material at 50 ~ 60 DEG C, carry out crushed 30 after cooling ~
40 meshes, vacuum, which saves, obtains Se-enriched feedstuff additive.
As a further improvement of the present invention, the preservative is benzoic acid, sodium benzoate, sorbic acid, dehydroacetic acid
One or more of combination.
As a further improvement of the present invention, the composite bacteria constituent and its weight fraction ratio are as follows: compound ferment
It is 15 ~ 20 parts of female bacterium, 8 ~ 10 parts of lactic acid bacteria, 6 ~ 8 parts of bacillus subtilis, 9 ~ 11 parts of bacillus licheniformis, 5 ~ 8 parts of aspergillus oryzae, black
6 ~ 10 parts of aspergillus, 8 ~ 10 parts of trichodermaharzianum.
As a further improvement of the present invention, the Chinese herbal medicine fluid is the preparation method comprises the following steps: according to parts by weight than taking Schisandra chinensis 8
~ 10 parts, 10 ~ 12 parts of creeping oxalis, fork divide 12 ~ 15 parts of knotweed, 8 ~ 10 parts of Fructus Corni, 6 ~ 8 parts of Chinese herbaceous peony mixing after carry out being ground into 10 mesh
Mixed-powder, into mixed-powder be added volume fraction be 50% ethyl alcohol make solid-liquid ratio be 1:3, then in ultrasonic power
It is progress 10 ~ 12min of ultrasonic extraction at 30 ~ 40 DEG C for 180 ~ 200W and temperature, is then centrifuged under the conditions of 5000r/min
Supernatant is taken to obtain Chinese herbal medicine fluid.
As a further improvement of the present invention, the mulriple yeasts preparation method are as follows:
A. it chooses strain: choosing 8 ~ 10 parts of saccharomyces carlsbergensis bacterium, 10 ~ 12 parts of candida utili bacterium, the torrid zone according to weight fraction ratio
12 ~ 15 parts of Candida, 8 ~ 10 parts of brewer's yeast, 6 ~ 12 parts of rhodotorula；
B. induction mutation of bacterium: by 8 ~ 10 parts of saccharomyces carlsbergensis bacterium, 10 ~ 12 parts of candida utili bacterium, 12 ~ 15 parts of candida tropicalis,
Picking mixed bacteria is inoculated on PDA slant medium after mixing for 8 ~ 10 parts of brewer's yeast, 6 ~ 12 parts of rhodotorula, at 30 DEG C
48h is cultivated, with the saccharomycete spore for passing through slant activation under sterile washing, is added in the triangular flask for having bead and is shaken
It is filtered after 20min with four layers of lens paper, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, takes 4mL's
Yeast bacterium suspension carries out 120 ~ 150s of ultraviolet irradiation mutagenesis in the sterilized culture dish with magnetic stirring bar, then will be through
The yeast bacterium suspension for crossing ultraviolet mutagenesis processing uses DES dilution 25 ~ 30min of mutagenic treatment again, is then rapidly added
10ml25% sodium thiosulfate terminates reaction and obtains mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by c. Zengjing Granule, and malt leaching is added
Powder culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
D. the barms of overnight incubation are inoculated into solid medium with 1% amount, 30 DEG C of 48 ~ 72h of static gas wave refrigerator are selected
The yeast strain to grow fine obtains mulriple yeasts.
As a further improvement of the present invention, the PDA culture medium formula and its preparation condition are potato 200g, Portugal
Grape sugar 20g, agar 15g, water 1000mL, natural ph, 121 DEG C, 0.1MPa sterilizing 15min.
As a further improvement of the present invention, the fructus hordei germinatus leaching powder liquid culture medium formula and its preparation condition are glucose
10g, peptone 2g, fructus hordei germinatus leaching powder 1g, yeast extract 1g, agar powder 12g, lactic acid 0.3mL/L, water 1000mL, 121 DEG C of sterilizings
30min。
As a further improvement of the present invention, the solid culture based formulas and its preparation condition are 0.1mm granularity
Moringa waste wood powder 20g, peptone 1g, glucose 2g, NaCl10g, agar powder 20g, aquae destillata 1000mL, pH5.5 ~ 6.0,121
DEG C sterilizing 30min.
A method of preparing selenium-rich ox feed, comprising the following steps:
(1) take 10 ~ 13 parts of sweet potato vine, 7 ~ 9 parts of straw be placed in pulverizer be ground into partial size be 30 ~ 40 mesh mixed-powder；
92) 12 ~ 15 parts of 16 ~ 18 parts of 18 ~ 20 parts of mixed-powder, corn flour, wheat flour, wheat bran, the grass meal obtained after taking (1) to crush
8 ~ 9 parts, 5 ~ 7 parts of rapeseed dregs, 8 ~ 10 parts of potato starch, 3 ~ 4 parts of molasses, 3 ~ 5 parts of sodium bisulfate, Se-enriched feedstuff additive 2 ~
4.5 parts, 10 ~ 12 parts of carboxymethyl cellulose, 1 ~ 2 part of salt, 0.5 ~ 1 part of citric acid, 1 ~ 2 part of medical stone, 2 ~ 4 parts of preservative stirrings
It is sent into pelletizer and is granulated after mixing, the pellet made is carried out obtained by drying to selenium-rich ox feed.
The technical principle that the present invention realizes is:
It is former based on corn flour, wheat flour, wheat bran, grass meal, rapeseed dregs, potato starch in selenium-rich ox feed of the invention
Material, by Se-enriched feedstuff additive into ox feed, can give the higher Se content of rear cattle and protein content, if in feed
The too many protein of content not only causes the waste of feed, and the beef cattle of raising can also be made not digest and assimilate so as to cause disease
Disease, addition is very few not to be able to satisfy beef cattle during the growth process to the demand of protein, and treated Se-enriched feedstuff additive adds
Entering feed can promote beef cattle that selenium and protein are rationally absorbed and utilized, to promote the healthy growth of beef cattle.Wheat in feed
Meal stone can promote the enterogastric peristalsis of rear cattle, adjust metabolism, promote the growth and development of rear cattle, citric acid is conducive to increase
The appetite for adding rear cattle improves the mouthfeel of feed.
In the preparation of Se-enriched feedstuff additive, using the integrated processes of mechanical activation and microbial fermentation to discarded Moringa
Branch carries out processing and prepares Moringa Se-enriched feedstuff additive.Chinese herbal medicine fluid, the medium-height grass of addition are added while carrying out mechanical activation
Medical fluid is acid Chinese herbal medicine fluid, is conducive to the proteolytic in Moringa wooden branch and leaf of Moringa in acid Chinese herbal medicine fluid.Through
It crosses the fiber macromolecular chain after mechanical activation in Moringa branch and leaf of Moringa and is interrupted destructurized, it is relatively small to form the degree of polymerization
Strand, the reticular structure of Moringa cellulose is by different degrees of destruction, and fiber is loose, and unformed area increases, specific surface
Product increases, and reactivity improves, and is conducive to microorganism and preferably degrades to Moringa, so that fermentation time is effectively reduced,
Improve the efficiency of microbial fermentation.By the interaction of Chinese herbal medicine fluid and mechanical ball mill, preferably make Moringa wooden branch and Moringa
It can be micro- in the step of nutriments such as the protein in leaf are leached out, and the protein being leached out is fermented below
Biogenic protease is digested so that containing the small molecule egg for being more conducive to cultivation livestock and poultry absorption in Se-enriched feedstuff additive
White peptide.In addition, Schisandra chinensis (Classification system: Schisandra chinensis), creeping oxalis (Latin in the Chinese herbal medicine fluid being added
: Oxalis corniculata L.), pitches and knotweed (Classification system: Polygonum divaricatum L.), Fructus Corni is divided (to draw
Fourth literary fame: Cornifructus), Chinese herbaceous peony (Classification system: Paeonia lactiflora Pall.) have antipyretic diuresis, detumescence
Scattered silt, sterilization and other effects, the functions of intestines and stomach of adjustable cultivation livestock and poultry, improve the immunity of rear cattle.
Carrying out mutagenic treatment to the yeast saccharomyces strain selected can be improved the fermentation activity of composite yeast strain, adaptability
And stability, to promote degradation of the barms to selenium-rich Moringa, so as to improve the nutritive value of selenium-rich Moringa.By producing protein
Candida, lactic acid bacteria fermentation, the selenium in Moringa can be carried out processing become organic selenium, be conducive to rear cattle to selenium
Absorption and utilization, improve the conversion ratio of selenium, the meat products obtained from are easier to be absorbed by the body, be it is a kind of safely and effectively
Method.Hay bacillus, brewer's yeast can generate a variety of digestive ferments such as protease, lipase and B family vitamin, improve selenium-rich
The utilization rate of feed addictive.The strains such as barms, bacillus subtilis, bacillus licheniformis is mutual during the fermentation
Effect can degrade to the alkaloid contained in Moringa, to reduce harm of the alkaloid to rear cattle, improve selenium-rich and raise
The safety of feed additives.Aspergillus niger and candida utili can be degraded the tannin in Moringa by fermentation, be reduced
The content of tannin in Se-enriched feedstuff additive improves selenium-rich and raises to eliminate influence of the tannin to Se-enriched feedstuff additive quality
The palatability of feed additives improves the feed intake of rear cattle.By the complementary symbiosis of the microorganism of addition, interaction is to make
The mycoprotein that low quality protein in selenium-rich Moringa is converted to high quality can be improved the albumen in Se-enriched feedstuff additive
Matter content also reduces coarse-fibred content, while a large amount of mycoproteins by accumulating in fermentation process and metabolism
Product can effectively improve the nutrient content of Se-enriched feedstuff additive.Glucose and lactose are added during the fermentation to be promoted
Into the growth of fermenting microbe, promote the progress of fermentation.
The present invention has following good result:
1. the selenium-rich ox feed organic selenium content that the present invention is prepared is high, nutritive value is high, and nutrition arrangement is balanced, can be effective
Promote absorption and utilization of the rear cattle to nutriment in feed, may advantageously facilitate the healthy growth of rear cattle, reduce feed
Additive amount, save the cost.
2. the content of organic selenium in beef can be increased after the Se-enriched feedstuff that the edible present invention is prepared, selenium-rich ox is eaten
Nutriment in beef effectively can be changed into nutriment needed for human body by meat descendant body.
3. the present invention is by handling Moringa waste wood to obtain Se-enriched feedstuff additive, at method of the invention
Se content abundant can be obtained after reason Moringa waste wood, while improving the content of protein in feed, additionally it is possible to reduce Moringa
The content of middle poisonous alkaloids avoids cultivation livestock and poultry poisoning to reduce harm of the poisonous alkaloids to cultivation livestock and poultry.
4. total Se content is 39.72 ~ 42.12 μ g/100g, organic selenium content in the selenium-rich ox feed that the present invention is prepared
For 34.63 ~ 35.17 μ g/100g, crude protein content is 24.79 ~ 26.27%, and crude fat content is 3.62 ~ 3.82%, and minerals contain
Amount is 4.19 ~ 4.38%；The nursing beef glossiness that the selenium-rich ox feed that the present invention is prepared is used for beef cattle is good, and meat is fresh
Perfume (or spice), resistance to aftertaste, Se content is 0.75 ~ 0.91mg/kg in beef, and beef cattle average daily gain improves 11.67 ~ 12.36%, feedstuff-meat ratio
Reduce by 5.36 ~ 6.54%.
Specific embodiment
The description present invention with reference to embodiments, these descriptions are not to be further limited to the content of present invention.
PDA culture medium formula and its preparation condition are as follows: potato 200g, glucose 20g, agar 15g, water 1000mL,
Natural ph, 121 DEG C, 0.1MPa sterilizing 15min.
Fructus hordei germinatus leaching powder liquid culture medium formula and its preparation condition are as follows: glucose 10g, peptone 2g, fructus hordei germinatus leaching powder 1g, ferment
Female cream 1g, agar powder 12g, lactic acid 0.3mL/L, water 1000mL, 121 DEG C of sterilizing 30min.
Solid culture based formulas and its preparation condition are as follows: for Moringa waste wood powder 20g, the peptone 1g, Portugal of 0.1mm granularity
1000mL, pH5.5 ~ 6.0,121 DEG C of grape sugar 2g, NaCl10g, agar powder 20g, aquae destillata sterilizing 30min.
Embodiment 1
Chinese herbal medicine fluid preparation: according to parts by weight than taking 8.5 parts of Schisandra chinensis, 12 parts of creeping oxalis, fork to divide 13.5 parts of knotweed, Fructus Corni
9.5 parts, 8 parts of Chinese herbaceous peony mixing after carry out the mixed-powder for being ground into 10 mesh, into mixed-powder be added volume fraction be 50% second
Alcohol makes solid-liquid ratio be 1:3, and ultrasonic extraction 11min is then carried out in the case where ultrasonic power is 180W and temperature is 40 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 9 parts of saccharomyces carlsbergensis bacterium, 11.5 parts of candida utili bacterium, the false silk ferment in the torrid zone according to weight fraction ratio
Female 15 parts, 9.5 parts of brewer's yeast, 12 parts of rhodotorula；
Induction mutation of bacterium: by 9 parts of saccharomyces carlsbergensis bacterium, 11.5 parts of candida utili bacterium, 15 parts of candida tropicalis, brewer's yeast
9.5 parts, picking mixed bacteria is inoculated on PDA slant medium 12 parts of rhodotorula after mixing, in 30 DEG C of culture 48h, use
Pass through the saccharomycete spore of slant activation under sterile washing, is added in the triangular flask for having bead after carrying out concussion 20min with four
Layer lens paper filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, and the saccharomycete of 4mL is taken to suspend
Liquid carries out ultraviolet irradiation mutagenesis 130s in the sterilized culture dish with magnetic stirring bar, then will be by ultraviolet mutagenesis
The yeast bacterium suspension of reason uses DES dilution mutagenic treatment 27min again, is then rapidly added the termination of 10ml25% sodium thiosulfate
Reaction obtains mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 65h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 385r/min and temperature is 70
It is taken out after DEG C carrying out activation 3h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the feed liquid after taking separation
It is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 5.5% of activation selenium-rich moringa powder quality is added
15 parts of mulriple yeasts, 9.5 parts of lactic acid bacteria, 6.5 parts of bacillus subtilis, 10 parts of bacillus licheniformis, 6 parts of aspergillus oryzae, black
7 parts of aspergillus, 0.8% glucose that activation selenium-rich moringa powder quality is added, is added and lives 8 parts of the trichodermaharzianum composite bacterias formed
0.5% lactose for changing selenium-rich moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.5, is uniformly mixed
Fermentation 4d is sealed at 30 DEG C afterwards, after fermentation dries material at 52 DEG C, carries out crushed 40 after cooling
Mesh, vacuum, which saves, obtains Se-enriched feedstuff additive；
Take 10 parts of sweet potato vine, 7.5 parts of straw be placed in pulverizer be ground into partial size be 35 mesh mixed-powder；
Take 8.2 parts of 13.5 parts of 17.5 parts of 19 parts of mixed-powder, corn flour, wheat flour, wheat bran, grass meal, the vegetable seed obtained after crushing
5 parts of the dregs of rice, 8.5 parts of potato starch, 3.8 parts of molasses, 4 parts of sodium bisulfate, 3 parts of Se-enriched feedstuff additive, carboxymethyl cellulose
11.5 parts, 1.5 parts of salt, 0.9 part of citric acid, 1.5 parts of medical stone, 3 parts of benzoic acid preservative be uniformly mixed after be sent into and make
It is granulated, the pellet made is carried out obtained by drying to selenium-rich ox feed in grain machine.
Embodiment 2
Chinese herbal medicine fluid preparation: according to parts by weight than take 9 parts of Schisandra chinensis, 11 parts of creeping oxalis, fork divide 12 parts of knotweed, 10 parts of Fructus Corni,
The mixed-powder for being ground into 10 mesh is carried out after 7.5 parts of Chinese herbaceous peony mixing, the ethyl alcohol that volume fraction is 50% is added into mixed-powder to be made
Obtaining solid-liquid ratio is 1:3, and ultrasonic extraction 10.5min is then carried out in the case where ultrasonic power is 195W and temperature is 32 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 8 parts of saccharomyces carlsbergensis bacterium, 12 parts of candida utili bacterium, candida tropicalis according to weight fraction ratio
13 parts, 9 parts of brewer's yeast, 8 parts of rhodotorula；
Induction mutation of bacterium: by 8 parts of saccharomyces carlsbergensis bacterium, 12 parts of candida utili bacterium, 13 parts of candida tropicalis, 9 parts of brewer's yeast,
Picking mixed bacteria is inoculated on PDA slant medium 8 parts of rhodotorula after mixing, in 30 DEG C of culture 48h, with sterile washing
The lower saccharomycete spore by slant activation is added in the triangular flask for having bead after carrying out concussion 20min with four layers of lens paper
Filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, takes the yeast bacterium suspension of 4mL in sterilized
Cingula, which has, carries out ultraviolet irradiation mutagenesis 120s in the culture dish of magnetic stirring bar, then by the yeast by ultraviolet mutagenesis processing
Bacterium suspension uses DES dilution mutagenic treatment 30min again, is then rapidly added the termination reaction of 10ml25% sodium thiosulfate and obtains
Mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 48h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 390r/min and temperature is 60
It is taken out after DEG C carrying out activation 2h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the feed liquid after taking separation
It is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 5.8% of activation selenium-rich moringa powder quality is added
20 parts of mulriple yeasts, 8 parts of lactic acid bacteria, 8 parts of bacillus subtilis, 9 parts of bacillus licheniformis, 8 parts of aspergillus oryzae, aspergillus niger 6
Part, 8.5 parts of the trichodermaharzianum composite bacterias formed, 0.5% glucose that activation selenium-rich moringa powder quality is added, addition activate
0.4% lactose of selenium-rich moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.7, after mixing
It is sealed fermentation 3d at 36 DEG C, after fermentation dries material at 60 DEG C, carries out crushed 45 mesh after cooling
Sieve, vacuum, which saves, obtains Se-enriched feedstuff additive；
Take 13 parts of sweet potato vine, 9 parts of straw be placed in pulverizer be ground into partial size be 32 mesh mixed-powder；
Take 9 parts of 13 parts of 18 parts of 20 parts of mixed-powder, corn flour, wheat flour, wheat bran, grass meal, the rapeseed dregs 6.5 obtained after crushing
Part, 9.5 parts of potato starch, 4 parts of molasses, 3.5 parts of sodium bisulfate, 3.5 parts of Se-enriched feedstuff additive, carboxymethyl cellulose 12
Part, 2 parts of salt, 0.6 part of citric acid, 1.8 parts of medical stone, benzoic acid and sorbic acid preservative totally 4 parts be uniformly mixed after give
Enter in pelletizer and be granulated, the pellet made is carried out obtained by drying to selenium-rich ox feed.
Embodiment 3
Chinese herbal medicine fluid preparation: according to parts by weight than taking 10 parts of Schisandra chinensis, 10.5 parts of creeping oxalis, fork to divide 13 parts of knotweed, Fructus Corni 9
The mixed-powder for being ground into 10 mesh is carried out after part, 6 parts of Chinese herbaceous peony mixing, the ethyl alcohol that volume fraction is 50% is added into mixed-powder
So that solid-liquid ratio is 1:3, ultrasonic extraction 11.5min is then carried out in the case where ultrasonic power is 200W and temperature is 30 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 9.5 parts of saccharomyces carlsbergensis bacterium, 11 parts of candida utili bacterium, the false silk ferment in the torrid zone according to weight fraction ratio
Female 12 parts, 8.5 parts of brewer's yeast, 10 parts of rhodotorula；
Induction mutation of bacterium: by 9.5 parts of saccharomyces carlsbergensis bacterium, 11 parts of candida utili bacterium, 12 parts of candida tropicalis, brewer's yeast
8.5 parts, picking mixed bacteria is inoculated on PDA slant medium 10 parts of rhodotorula after mixing, in 30 DEG C of culture 48h, use
Pass through the saccharomycete spore of slant activation under sterile washing, is added in the triangular flask for having bead after carrying out concussion 20min with four
Layer lens paper filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, and the saccharomycete of 4mL is taken to suspend
Liquid carries out ultraviolet irradiation mutagenesis 140s in the sterilized culture dish with magnetic stirring bar, then will be by ultraviolet mutagenesis
The yeast bacterium suspension of reason uses DES dilution mutagenic treatment 26min again, is then rapidly added the termination of 10ml25% sodium thiosulfate
Reaction obtains mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 54h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 380r/min and temperature is 75
It is taken out after DEG C carrying out activation 2.8h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the material after taking separation
Liquid is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 5.2% of activation selenium-rich moringa powder quality is added
16 parts of mulriple yeasts, 10 parts of lactic acid bacteria, 7 parts of bacillus subtilis, 10.5 parts of bacillus licheniformis, 7 parts of aspergillus oryzae, black song
The composite bacteria of mould 8 parts, 10 parts of trichodermaharzianum compositions, 0.6% glucose that activation selenium-rich moringa powder quality is added are added and live
0.3% lactose for changing selenium-rich moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:2.0, is uniformly mixed
Fermentation 4d is sealed at 34 DEG C afterwards, after fermentation dries material at 50 DEG C, carries out crushed 30 after cooling
Mesh, vacuum, which saves, obtains Se-enriched feedstuff additive；
Take 11.5 parts of sweet potato vine, 8 parts of straw be placed in pulverizer be ground into partial size be 30 mesh mixed-powder；
Take 8.5 parts of 14 parts of 16 parts of 19.5 parts of mixed-powder, corn flour, wheat flour, wheat bran, grass meal, the rapeseed dregs 6 obtained after crushing
Part, 9 parts of potato starch, 3.5 parts of molasses, 5 parts of sodium bisulfate, 4.5 parts of Se-enriched feedstuff additive, 11 parts of carboxymethyl cellulose,
1.2 parts of salt, 0.8 part of citric acid, 1 part of medical stone, 3.5 parts of dehydroacetic acid preservative be uniformly mixed after be sent into pelletizer in
It is granulated, the pellet made is carried out obtained by drying to selenium-rich ox feed.
Embodiment 4
Chinese herbal medicine fluid preparation: according to parts by weight than take 9.5 parts of Schisandra chinensis, 10 parts of creeping oxalis, fork divide 14 parts of knotweed, 8 parts of Fructus Corni,
The mixed-powder for being ground into 10 mesh is carried out after 6.5 parts of Chinese herbaceous peony mixing, the ethyl alcohol that volume fraction is 50% is added into mixed-powder to be made
Obtaining solid-liquid ratio is 1:3, and ultrasonic extraction 10min is then carried out in the case where ultrasonic power is 185W and temperature is 35 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 10 parts of saccharomyces carlsbergensis bacterium, 10.5 parts of candida utili bacterium, the false silk ferment in the torrid zone according to weight fraction ratio
Female 14 parts, 8 parts of brewer's yeast, 6 parts of rhodotorula；
Induction mutation of bacterium: by 10 parts of saccharomyces carlsbergensis bacterium, 10.5 parts of candida utili bacterium, 14 parts of candida tropicalis, brewer's yeast 8
Part, picking mixed bacteria is inoculated on PDA slant medium 6 parts of rhodotorula after mixing, and in 30 DEG C of culture 48h, use is sterile
The lower saccharomycete spore Jing Guo slant activation of washing, is added in the triangular flask for having bead after carrying out concussion 20min with four layers of mirror
Head paper filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, take the yeast bacterium suspension of 4mL in
Ultraviolet irradiation mutagenesis 135s is carried out in the sterilized culture dish with magnetic stirring bar, it then will be by ultraviolet mutagenesis processing
Yeast bacterium suspension uses DES dilution mutagenic treatment 25min again, is then rapidly added 10ml25% sodium thiosulfate and terminates reaction
Obtain mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 72h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 400r/min and temperature is 80
It is taken out after DEG C carrying out activation 2.5h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the material after taking separation
Liquid is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 5% of activation selenium-rich moringa powder quality is added
17 parts of mulriple yeasts, 9 parts of lactic acid bacteria, 7.5 parts of bacillus subtilis, 11 parts of bacillus licheniformis, 5 parts of aspergillus oryzae, aspergillus niger
1% glucose that activation selenium-rich moringa powder quality is added, activation is added in the composite bacteria of 10 parts, 9.5 parts of trichodermaharzianum compositions
0.2% lactose of selenium-rich moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.6, after mixing
It is sealed fermentation 3.5d at 27 DEG C, after fermentation dries material at 58 DEG C, carries out crushed 42 after cooling
Mesh, vacuum, which saves, obtains Se-enriched feedstuff additive；
Take 12 parts of sweet potato vine, 7 parts of straw be placed in pulverizer be ground into partial size be 40 mesh mixed-powder；
Take 8 parts of 15 parts of 16.5 parts of 18 parts of mixed-powder, corn flour, wheat flour, wheat bran, grass meal, the rapeseed dregs 5.5 obtained after crushing
Part, 10 parts of potato starch, 3.2 parts of molasses, 3 parts of sodium bisulfate, 4 parts of Se-enriched feedstuff additive, 10.5 parts of carboxymethyl cellulose,
1 part of salt, 0.5 part of citric acid, 2 parts of medical stone, 2.5 parts of sorbic acid preservative be uniformly mixed after be sent into pelletizer in carry out
It is granulated, the pellet made is carried out obtained by drying to selenium-rich ox feed.
Embodiment 5
Chinese herbal medicine fluid preparation: according to parts by weight than taking 8 parts of Schisandra chinensis, 11.5 parts of creeping oxalis, fork to divide 15 parts of knotweed, Fructus Corni 8.5
The mixed-powder for being ground into 10 mesh is carried out after part, 7 parts of Chinese herbaceous peony mixing, the ethyl alcohol that volume fraction is 50% is added into mixed-powder
So that solid-liquid ratio is 1:3, ultrasonic extraction 12min is then carried out in the case where ultrasonic power is 190W and temperature is 38 DEG C, is then existed
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid；
It chooses strain: choosing 8.5 parts of saccharomyces carlsbergensis bacterium, 10 parts of candida utili bacterium, the false silk ferment in the torrid zone according to weight fraction ratio
Female 13 parts, 10 parts of brewer's yeast, 9 parts of rhodotorula；
Induction mutation of bacterium: by 8.5 parts of saccharomyces carlsbergensis bacterium, 10 parts of candida utili bacterium, 13 parts of candida tropicalis, brewer's yeast 10
Part, picking mixed bacteria is inoculated on PDA slant medium 9 parts of rhodotorula after mixing, and in 30 DEG C of culture 48h, use is sterile
The lower saccharomycete spore Jing Guo slant activation of washing, is added in the triangular flask for having bead after carrying out concussion 20min with four layers of mirror
Head paper filtering, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, take the yeast bacterium suspension of 4mL in
Ultraviolet irradiation mutagenesis 150s is carried out in the sterilized culture dish with magnetic stirring bar, it then will be by ultraviolet mutagenesis processing
Yeast bacterium suspension uses DES dilution mutagenic treatment 28min again, is then rapidly added 10ml25% sodium thiosulfate and terminates reaction
Obtain mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by Zengjing Granule, and fructus hordei germinatus leaching powder is added
Culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
The barms of overnight incubation are inoculated into solid medium with 1% amount, it is good to select growing way by 30 DEG C of static gas wave refrigerator 60h
Good yeast strain obtains mulriple yeasts；
Mechanical activation pretreatment: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, and selenium-rich is peppery
The discarded wooden branch of wood and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and it is 1:2 that mass ratio is added in the ball mill:
1.5 selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution, and make selenium-rich moringa powder, high aluminium ball ball-milling medium and
80% that total volume is ball mill volume is added in Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 390r/min and temperature is 65
It is taken out after DEG C carrying out activation 2.2h, feed liquid after treatment is separated with high aluminium ball ball-milling medium, the material after taking separation
Liquid is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and the 6% of activation selenium-rich moringa powder quality is added
18 parts of mulriple yeasts, 8.5 parts of lactic acid bacteria, 6 parts of bacillus subtilis, 9.5 parts of bacillus licheniformis, 6 parts of aspergillus oryzae, aspergillus niger
The composite bacteria of 9 parts, 9 parts of trichodermaharzianum compositions, 0.7% glucose that activation selenium-rich moringa powder quality is added, addition activation are rich
0.5% lactose of selenium moringa powder quality, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.8, is existed after mixing
It is sealed fermentation 3d at 32 DEG C, after fermentation dries material at 55 DEG C, carries out crushed 48 meshes after cooling,
Vacuum, which saves, obtains Se-enriched feedstuff additive；
Take 11 parts of sweet potato vine, 8.5 parts of straw be placed in pulverizer be ground into partial size be 35 mesh mixed-powder；
Take 8.8 parts of 12 parts of 17 parts of 18.5 parts of mixed-powder, corn flour, wheat flour, wheat bran, grass meal, the rapeseed dregs 7 obtained after crushing
Part, 8 parts of potato starch, 3 parts of molasses, 4.5 parts of sodium bisulfate, 2 parts of Se-enriched feedstuff additive, 10 parts of carboxymethyl cellulose, food
1.8 parts of salt, 1 part of citric acid, 1.2 parts of medical stone, benzoic acid and dehydroacetic acid preservative totally 2 parts be uniformly mixed after be sent into and make
It is granulated, the pellet made is carried out obtained by drying to selenium-rich ox feed in grain machine.
In order to verify the selenium-rich ox quality of the fodder that the present invention is prepared, the embodiment of the present invention 1 ~ 5 is prepared
Selenium-rich ox feed carries out the measurement of the indexs such as crude protein, crude fat, while using commercially available common ox feed as control group.Index is surveyed
It is as shown in table 1 below to try situation.
1 selenium-rich ox feed index test situation of table
The selenium-rich ox feed that the embodiment of the present invention 1 ~ 5 is prepared is used for the nursing of beef cattle, carries out within 30 days before beef cattle is delivered for sale
It feeds, is conventionally fed, observe the indexs such as the Se content of beef cattle, while control group is set, deliver 3 for sale in beef cattle
Normal diet is fed before it, and it is as shown in table 2 below to feed situation.
2 Se-enriched feedstuff of table feeds beef cattle situation
The above embodiment of the present invention scheme is only the description of the invention and cannot limit the present invention, this is indicated in claim
Invention product form ingredient, component ratio, the range of preparation method parameter, and above-mentioned explanation does not point out parameter of the present invention
Range, therefore, any change within the meaning and scope equivalent to the claims of the present invention, be all considered to be including
Within the scope of the claims.
The present invention is created by multidigit feed processing technology personnel's long-term work experience accumulation, and by creative work
And go out, the selenium-rich ox feed nutrient that the present invention is prepared is abundant, and protein content is high, and organic selenium content height is conducive to meat
The absorption and utilization of ox, while may advantageously facilitate the growth of ox, improve feeding efficiency.
Claims (9)
1. a kind of selenium-rich ox feed, characterized in that each constituent of selenium-rich ox feedstuff and its weight fraction ratio are as follows: corn
18 ~ 20 parts of powder, 16 ~ 18 parts of wheat flour, 12 ~ 15 parts of wheat bran, 10 ~ 13 parts of sweet potato vine, 7 ~ 9 parts of straw, 8 ~ 9 parts of grass meal, rapeseed dregs 5
~ 7 parts, 8 ~ 10 parts of potato starch, 3 ~ 4 parts of molasses, 3 ~ 5 parts of sodium bisulfate, 2 ~ 4.5 parts of Se-enriched feedstuff additive, carboxymethyl fibre
Tie up plain 10 ~ 12 parts, 1 ~ 2 part of salt, 0.5 ~ 1 part of citric acid, 1 ~ 2 part of medical stone, 2 ~ 4 parts of preservative；
The Se-enriched feedstuff addictive preparation method are as follows:
The pretreatment of step 1) mechanical activation: being that 2:1 takes selenium-rich Moringa to discard wooden branch and selenium-rich leaf of Moringa respectively according to mass ratio, will
The discarded wooden branch of selenium-rich Moringa and selenium-rich leaf of Moringa are crushed to obtain selenium-rich moringa powder after drying, and mass ratio is added in the ball mill
Selenium-rich moringa powder, high aluminium ball ball-milling medium, Chinese herbal medicine solution for 1:2:1.5, and make selenium-rich moringa powder, high aluminium ball ball milling
80% that total volume is ball mill volume is added in medium and Chinese herbal medicine solution, and then adjusting drum's speed of rotation is 380 ~ 400r/min
It is taken out after carrying out 2 ~ 3h of activation with temperature for 60 ~ 80 DEG C, feed liquid after treatment is separated with high aluminium ball ball-milling medium,
Feed liquid after taking separation is evaporated in vacuo to obtain activation selenium-rich moringa powder；
Step 2) fermentation process: obtained activation selenium-rich moringa powder is placed in fermentation cabin, and activation selenium-rich moringa powder quality is added
5 ~ 6% composite bacteria, be added activation selenium-rich moringa powder quality 0.5 ~ 1% glucose, be added activation selenium-rich Moringa silty
0.2 ~ 0.5% lactose of amount, finally adding sterile water and adjusting material-water ratio (m/V) is 1:1.5 ~ 1:2.0, is existed after mixing
It is sealed 3 ~ 4d of fermentation at 27 ~ 36 DEG C, after fermentation dries material at 50 ~ 60 DEG C, is crushed after cooling
30 ~ 40 meshes are crossed, vacuum, which saves, obtains Se-enriched feedstuff additive.
2. a kind of selenium-rich ox feed according to claim 1, characterized in that the preservative is benzoic acid, benzoic acid
The combination of one or more of sodium, sorbic acid, dehydroacetic acid.
3. a kind of selenium-rich ox feed according to claim 1, characterized in that the composite bacteria constituent and its again
Measure portion rate are as follows: 15 ~ 20 parts of mulriple yeasts, 8 ~ 10 parts of lactic acid bacteria, 6 ~ 8 parts of bacillus subtilis, bacillus licheniformis 9 ~ 11
Part, 5 ~ 8 parts of aspergillus oryzae, 6 ~ 10 parts of aspergillus niger, 8 ~ 10 parts of trichodermaharzianum.
4. a kind of selenium-rich ox feed according to claim 1, characterized in that the Chinese herbal medicine fluid the preparation method comprises the following steps: according to
Weight fraction ratio take 8 ~ 10 parts of Schisandra chinensis, 10 ~ 12 parts of creeping oxalis, fork divide 12 ~ 15 parts of knotweed, 8 ~ 10 parts of Fructus Corni, 6 ~ 8 parts of Chinese herbaceous peony it is mixed
The mixed-powder for being ground into 10 mesh is carried out after conjunction, and the ethyl alcohol that volume fraction is 50% is added into mixed-powder and makes the solid-liquid ratio to be
Then 1:3 carries out 10 ~ 12min of ultrasonic extraction in the case where ultrasonic power is 180 ~ 200W and temperature is 30 ~ 40 DEG C, then exists
Centrifuging and taking supernatant is carried out under the conditions of 5000r/min obtains Chinese herbal medicine fluid.
5. a kind of selenium-rich ox feed according to claim 1, characterized in that the mulriple yeasts preparation method are as follows:
A. it chooses strain: choosing 8 ~ 10 parts of saccharomyces carlsbergensis bacterium, 10 ~ 12 parts of candida utili bacterium, the torrid zone according to weight fraction ratio
12 ~ 15 parts of Candida, 8 ~ 10 parts of brewer's yeast, 6 ~ 12 parts of rhodotorula；
B. induction mutation of bacterium: by 8 ~ 10 parts of saccharomyces carlsbergensis bacterium, 10 ~ 12 parts of candida utili bacterium, 12 ~ 15 parts of candida tropicalis,
Picking mixed bacteria is inoculated on PDA slant medium after mixing for 8 ~ 10 parts of brewer's yeast, 6 ~ 12 parts of rhodotorula, at 30 DEG C
48h is cultivated, with the saccharomycete spore for passing through slant activation under sterile washing, is added in the triangular flask for having bead and is shaken
It is filtered after 20min with four layers of lens paper, it is appropriate to dilute, make spore concentration 106A/mL obtains yeast bacterium suspension, takes 4mL's
Yeast bacterium suspension carries out 120 ~ 150s of ultraviolet irradiation mutagenesis in the sterilized culture dish with magnetic stirring bar, then will be through
The yeast bacterium suspension for crossing ultraviolet mutagenesis processing uses DES dilution 25 ~ 30min of mutagenic treatment again, is then rapidly added
10ml25% sodium thiosulfate terminates reaction and obtains mutagenesis barms；
Mutagenesis barms: being aseptically added in the triangular flask with bead by c. Zengjing Granule, and malt leaching is added
Powder culture medium, 180r/min, shaken cultivation is stayed overnight at 30 DEG C；
D. the barms of overnight incubation are inoculated into solid medium with 1% amount, 30 DEG C of 48 ~ 72h of static gas wave refrigerator are selected
The yeast strain to grow fine obtains mulriple yeasts.
6. a kind of selenium-rich ox feed according to claim 4, characterized in that the PDA culture medium formula and its preparation
Condition be potato 200g, glucose 20g, agar 15g, water 1000mL, natural ph, 121 DEG C, 0.1MPa sterilize 15min.
7. a kind of selenium-rich ox feed according to claim 4, characterized in that the fructus hordei germinatus leaching powder liquid culture medium formula and
Its preparation condition is glucose 10g, peptone 2g, fructus hordei germinatus leaching powder 1g, yeast extract 1g, agar powder 12g, lactic acid 0.3mL/L, water
1000mL, 121 DEG C of sterilizing 30min.
8. a kind of selenium-rich ox feed according to claim 4, characterized in that the solid culture based formulas and its preparation
Condition is Moringa waste wood powder 20g, peptone 1g, the glucose 2g, NaCl10g, agar powder 20g, aquae destillata of 0.1mm granularity
1000mL, pH5.5 ~ 6.0,121 DEG C of sterilizing 30min.
9. a kind of method for preparing selenium-rich ox feed any one of claims 1 to 8, characterized in that including following step
It is rapid:
Step 1) take 10 ~ 13 parts of sweet potato vine, 7 ~ 9 parts of straw be placed in pulverizer be ground into partial size be 30 ~ 40 mesh mixed-powder；
16 ~ 18 parts of 18 ~ 20 parts of mixed-powder, corn flour, wheat flour, the wheat bran 12 ~ 15 that step 2) obtains after taking step 1) to crush
Part, 8 ~ 9 parts of grass meal, 5 ~ 7 parts of rapeseed dregs, 8 ~ 10 parts of potato starch, 3 ~ 4 parts of molasses, 3 ~ 5 parts of sodium bisulfate, Se-enriched feedstuff add
Add 2 ~ 4.5 parts of agent, 10 ~ 12 parts of carboxymethyl cellulose, 1 ~ 2 part of salt, 0.5 ~ 1 part of citric acid, 1 ~ 2 part of medical stone, preservative 2 ~ 4
Part is sent into pelletizer after being uniformly mixed and is granulated, and the pellet made is carried out obtained by drying to the feeding of selenium-rich ox
Material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811235234.9A CN109362956A (en) | 2018-10-23 | 2018-10-23 | A kind of selenium-rich ox feed and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110679545A (en) * | 2019-11-15 | 2020-01-14 | 陈武茂 | Beef cattle ecological breeding method |
| CN112868941A (en) * | 2021-02-26 | 2021-06-01 | 海南晨海水产有限公司 | Selenium-rich feed suitable for feeding siganus oramin and preparation method |
-
2018
- 2018-10-23 CN CN201811235234.9A patent/CN109362956A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110679545A (en) * | 2019-11-15 | 2020-01-14 | 陈武茂 | Beef cattle ecological breeding method |
| CN112868941A (en) * | 2021-02-26 | 2021-06-01 | 海南晨海水产有限公司 | Selenium-rich feed suitable for feeding siganus oramin and preparation method |
| CN112868941B (en) * | 2021-02-26 | 2023-03-31 | 海南晨海水产有限公司 | Selenium-rich feed suitable for feeding siganus oramin and preparation method |
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