CN106343410A - Production method of kelp extract - Google Patents
Production method of kelp extract Download PDFInfo
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- CN106343410A CN106343410A CN201610815088.1A CN201610815088A CN106343410A CN 106343410 A CN106343410 A CN 106343410A CN 201610815088 A CN201610815088 A CN 201610815088A CN 106343410 A CN106343410 A CN 106343410A
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- thallus laminariae
- thallus eckloniae
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Abstract
The invention discloses a production method of a kelp extract. The production method includes steps of kelp cleaning, smashing, hydrolyzing, enzymolysis, filtering, filtrate concentration, and low-temperature spraying and drying. The content of algal polysaccharide in kelp extract dry powder prepared by the method is over 35%, and the kelp extract dry powder contains rich trace elements and mineral matters, and other natural active matters. A product of the kelp extract is high in algal polysaccharide content, good in quality, excellent in stability and high in natural active substance content. Compared with a traditional method, the production method has advantages of low energy consumption and short time in a production process; therefore, the production method is a production technology which is advanced in preparation process, high in efficiency, environmental friendly, hygienic and remarkable in economic benefit.
Description
Technical field
The invention belongs to marine organisms production and processing extractive technique field and in particular to a kind of Thallus Laminariae (Thallus Eckloniae) extract production
Method.
Background technology
Thallus Laminariae (Thallus Eckloniae), one of seaweed plant, it is a kind of raw Brown algae plant in large-scale sea of growth in low temperature seawater, belong to Brown algae
Door cotton material, is Zosteraceae plant.It is the thalluves of Laminariaceae plant sea-tangle.Because it is grown in sea water, pliable and tough like band and gain the name.
Shandong, Liaoning, Zhejiang, Fujian, Coast of Guangdong Province are distributed or artificial cultivation.Thallus Laminariae (Thallus Eckloniae) is a kind of very high vegetable of nutritive value, with
When there is certain medical value, Thallus Laminariae (Thallus Eckloniae) contain phycocolloid acid, laminaran, Mannitol, galactan, sea-tangle glycan, laminine, oxygen
Change potassium, iodine, calcium, cobalt, fluorine, carotene, vitamin b1, b2, c, p, protein, the amino such as proline, glutamic acid, aspartic acid
Acid;Seaweed extracted liquor proteoglycan can resist various viruses, Sargassum polysaccharides and the special marine active substance of oligochitosan both
There is toxin expelling and the effect of reinforced immunological function, suppression tumor.According to science measure: in every 100 grams of Thallus Laminariae (Thallus Eckloniae)s contain 8 grams of protein,
Fat 0.1 gram, 0.57 milligram of carotene, 0.09 milligram of vitamin b1,0.36 milligram of b2,1.6 milligrams of nicotinic acid, 50 milligrams of ferrum,
216 milligrams of phosphorus, cobalt 22 microgram and a certain amount of vitamin c etc..Its sugar, calcium, the content of ferrum exceed Herba Spinaciae, several times of Brassica campestris L to several
Ten times.In food, the amount of iodine of Thallus Laminariae (Thallus Eckloniae) is very high, the just iodine containing 300-700 milligram in every 100 grams of Thallus Laminariae (Thallus Eckloniae)s.Iodine is in human body
A kind of necessary trace element, is one of important component of synthetic thyroid.If human body is chronically at the state of iodine deficiency, will
Cause thyromegaly, thus affecting healthy.Therefore often eat Thallus Laminariae (Thallus Eckloniae) and can supplement required iodine for body in time, prevent
The generation of thyromegaly disease.And anemia of pregnant woman more should suitably have some Thallus Laminariae (Thallus Eckloniae), the intake of supplementing iodine, in order to avoid cause fetus
Dysplasia, mental retardation.There is expert to think simultaneously, because people are daily, animal proteinum, lean meat, white sugar etc. taken in excessive,
It is easily caused thyroid gland deficiency and skin metabolism obstacle, forms poliosis, it is therefore proposed that often eating Thallus Laminariae (Thallus Eckloniae), to prevent or to reduce hair
Bleach.The traditional Chinese medical science thinks, Thallus Laminariae (Thallus Eckloniae) nature and flavor are salty-cold, nontoxic, has hard masses softening and resolving, eliminating phlegm to relieve asthma, the current work(such as diuresis, blood fat-reducing blood pressure-decreasing
Effect, often eats Thallus Laminariae (Thallus Eckloniae) to healthy highly advantageous.Boil soup as Thallus Laminariae (Thallus Eckloniae) to eat, often eat and can control children hypertension, coronary heart disease;Thallus Laminariae (Thallus Eckloniae) is cooked
Or cold and dressed with sauce, often eat and can control goiter;And Thallus Laminariae (Thallus Eckloniae) and different food pairings, also more effects, such as Thallus Laminariae (Thallus Eckloniae), glutinous rice, Semen phaseoli radiati,
Brown sugar is each appropriate, cooks congee edible, can control skin noxious dampness and scratch where it itches;500 grams of dried kelp, clean stripping and slicing, boil and pull out, plus white sugar
Mix thoroughly for 250 grams, edible after salting down 24 hours, each serving 50 grams, take day secondary, chronic pharyngitiss can be controlled;Thallus Laminariae (Thallus Eckloniae) can be with cough-relieving.Thallus Laminariae (Thallus Eckloniae) is
A kind of basic food, often eating to increase the absorption to calcium for the human body, be doped into a Thallus Laminariae (Thallus Eckloniae), can reduce in greasy excessive food
Fat accumulation in vivo.It is all highly beneficial to the healthy of people.In order that Thallus Laminariae (Thallus Eckloniae) plays more preferable economic benefit
With the health benefit of wide spectrum, using intensive processing, extract laminarin isoreactivity material extract, Jin Erkai with biotechnology
Send out food, health food and medicine and there is significant meaning.
Content of the invention
It is an object of the invention to provide a kind of production method of Thallus Laminariae (Thallus Eckloniae) extract, sea in prepared Thallus Laminariae (Thallus Eckloniae) extract dry powder
Polysaccharides content is more than 35%, and contains the natural active matters such as abundant trace element and mineral.In the product of the present invention
Sargassum polysaccharides content is high, quality better, product good stability, natural active matter rich content.Compare than traditional method, this
The power consumption of bright production process is low, the time is short, is that preparation technology is advanced, high-efficiency environment friendly health, remarkable in economical benefits production skill
Art.
For achieving the above object, the present invention adopts the following technical scheme that
A kind of production method of Thallus Laminariae (Thallus Eckloniae) extract, comprises the following steps: Thallus Laminariae (Thallus Eckloniae) cleaning, pulverizing, hydrolysis, enzymolysis, filtration, filtrate are dense
Contracting, low temperature spray drying.
Specifically comprise the following steps that
Step 1: Thallus Laminariae (Thallus Eckloniae) cleaning: remove soil, the impurity on Thallus Laminariae (Thallus Eckloniae) surface by cleaning;
Step 2: pulverize: the Thallus Laminariae (Thallus Eckloniae) cleaning up is pulverized, smashing fineness is 5-30 mesh;
Step 3: hydrolysis: by weight mixing Thallus Laminariae (Thallus Eckloniae) ground product with water for 1:10-15, stir, hydrolyze 2h;
Step 4: enzymolysis: percentage: add Digestive Enzyme 1.0%, proteinase-10 .5%, cellulose in Thallus Laminariae (Thallus Eckloniae) hydrolyzed solution
Enzyme 0.5%, pectase 1.0%, adjust ph=6-8,55-60 DEG C of enzymolysis 3-4h;Described lipase activity power is 1.5 × 105u/
Kg, proteinase activity power is 2 × 105U/kg, cellulose enzyme vigor is 10 × 105U/kg, pectase enzyme activity is 3 × 105u/
kg;Enzymolysis terminates, and when ph value is down to 4-5, feed liquid is warming up to 100 DEG C, and enzyme denaturing 2-3min obtains enzymolysis solution;
Step 5: filter: by enzymolysis solution first through colloid mill homogenizing, rotating speed 800-1000r/min is ground in rotation, then through high pressure homogenizer
Homogenizing, pressure 40-60mpa, finally filters, 200 keevil frames, filter pressure 20-30mpa, flow 0.5-1.0t/h, obtains filtrate;
Filtering residue is as agricultural fertilizer;
Step 6: filtrate concentrates: adopts dual-effect concentrator, evaporating temperature 55-70 DEG C, steam pressure 0.25mpa, filtrate is concentrated
To concentration brix=20-25%;
Step 7: low temperature spray drying: using vacuum and low temperature liquid continuous drier, temperature≤60-70 DEG C, evaporated quantity of water 65-
75kg/h, obtains Thallus Laminariae (Thallus Eckloniae) extract dry powder.
The remarkable advantage of the present invention is: in prepared Thallus Laminariae (Thallus Eckloniae) extract dry powder, Sargassum polysaccharides content, more than 35%, and contains
There are the natural active matters such as abundant trace element and mineral.Sargassum polysaccharides content height, quality better, system in the product of the present invention
Product good stability, natural active matter rich content.Compare than traditional method, production process power consumption of the present invention is low, the time is short,
It is that preparation technology is advanced, the production technology of high-efficiency environment friendly health, remarkable in economical benefits.
Specific embodiment
A kind of production method of Thallus Laminariae (Thallus Eckloniae) extract, the step of its preparation method is as follows:
Step 1: cleaning.Cleaning Thallus Laminariae (Thallus Eckloniae), removes soil, impurity;
Step 2: pulverize.Thallus Laminariae (Thallus Eckloniae) is pulverized, smashing fineness is 5-30 mesh;
Step 3: hydrolysis.By the Thallus Laminariae (Thallus Eckloniae) ground product of 1 weight portion, mix with 10-15 weight portion water, form mixed liquor.Stirring is all
Even, hydrolysis time 2h.
Step 4: enzymolysis.Digestive Enzyme 1.0wt%, proteinase-10 .5wt%, cellulase is added in Thallus Laminariae (Thallus Eckloniae) ground product hydrolyzed solution
0.5wt%, pectase 1.0wt%.Temperature is maintained at 55-60 DEG C, ph6-8, enzymolysis time 3-4h.Described lipase activity power is
1.5×105U/kg, proteinase activity power is 2 × 105U/kg, cellulose enzyme vigor is 10 × 105U/kg, pectase enzyme activity
For 3 × 105U/kg, temperature is maintained at 55-60 DEG C, enzymolysis time 3-4h.After enzyme digestion reaction, when ph value drops to ph4-5, by feed liquid
It is warming up to 100 DEG C, enzyme denaturing 2-3min, obtain enzymolysis solution.
Step 5: filter.Solution will be digested first through colloid mill homogenizing, rotation mill rotating speed 800-1000r/min.Make material
Grain disintegrate, reaches homogenizing, then through high pressure homogenizer homogenizing (pressure 40mpa-60mpa), makes enzymolysis solution pass through 200 keevil frame mistakes
Filter.Plate filter is stainless steel multilayer plate and frame type filter-press, filter pressure 20-30mpa, flow 0.5-1.0t/h.Obtain filtrate.
Filtering residue is Thallus Laminariae (Thallus Eckloniae) slag charge, can use as agricultural fertilizer etc..
Step 6: filtrate concentrates, and using dual-effect concentrator, evaporating temperature 55-70 DEG C, steam pressure 0.25mpa, by filtrate
Liquid concentration is to concentration brix=20-25%.
Step 7: using vacuum and low temperature liquid continuous drier low temperature spray drying, temperature≤60-70 DEG C, evaporated quantity of water
65-75kg/h, the laminarin isoreactivity extract dry powder obtaining.(dry powder observed watercut is≤5.0%).
Embodiment 1
A kind of production method of Thallus Laminariae (Thallus Eckloniae) extract, the step of its preparation method is as follows:
Step 1: cleaning.Take Fresh Laminaria Japonica 50kg, clean Thallus Laminariae (Thallus Eckloniae), remove soil, impurity;
Step 2: pulverize.Thallus Laminariae (Thallus Eckloniae) is pulverized, smashing fineness is 5-30 mesh;
Step 3: hydrolysis.By Thallus Laminariae (Thallus Eckloniae) ground product 50kg, mix with 10 weight portion water 500kg, form mixed liquor.Stir, water
Solution time 2h.
Step 4: enzymolysis.Digestive Enzyme 1.0wt%, proteinase-10 .5wt%, cellulase is added in Thallus Laminariae (Thallus Eckloniae) ground product hydrolyzed solution
0.5wt%, pectase 1.0wt%.Temperature is maintained at 55-60 DEG C, ph6-8, enzymolysis time 3-4h.Described lipase activity power is
1.5×105U/kg, proteinase activity power is 2 × 105U/kg, cellulose enzyme vigor is 10 × 105U/kg, pectase enzyme activity
For 3 × 105U/kg, temperature is maintained at 55-60 DEG C, enzymolysis time 3-4h.After enzyme digestion reaction, when ph value drops to ph4-5, by feed liquid
It is warming up to 100 DEG C, enzyme denaturing 2-3min, obtain enzymolysis solution.
Step 5: filter.Solution will be digested first through colloid mill homogenizing, rotation mill rotating speed 800-1000r/min.Make material
Grain disintegrate, reaches homogenizing, then through high pressure homogenizer homogenizing (pressure 40mpa-60mpa), makes enzymolysis solution pass through 200 keevil frame mistakes
Filter.Plate filter is stainless steel multilayer plate and frame type filter-press, filter pressure 20-30mpa, flow 0.5-1.0t/h.Obtain filtrate.
Filtering residue is Thallus Laminariae (Thallus Eckloniae) slag charge, can use as agricultural fertilizer etc..
Step 6: filtrate concentrates, and using dual-effect concentrator, evaporating temperature 55-70 DEG C, steam pressure 0.25mpa, by filtrate
Liquid concentration is to concentration brix=20-25%.
Step 7: using vacuum and low temperature liquid continuous drier low temperature spray drying, temperature≤60-70 DEG C, evaporated quantity of water
65-75kg/h, the laminarin isoreactivity extract dry powder obtaining.Obtain Thallus Laminariae (Thallus Eckloniae) extract active substance dry powder meter through measuration
8.215kg, extracting yield is 16.43%.(dry powder observed watercut is 5.0%).
Repeat production by the method for above-described embodiment 1 and proportioning:
Embodiment 2: take Fresh Laminaria Japonica 100kg, repeat production by above-mentioned embodiment party's example 1 method and proportioning, obtain Thallus Laminariae (Thallus Eckloniae) and extract
Active substances dry powder meter 17.150kg, extracting yield is 17.15%.(dry powder observed watercut is 5.0%).
Embodiment 3: take Fresh Laminaria Japonica 200kg, repeat production by above-mentioned embodiment party's example 1 method and proportioning, obtain Thallus Laminariae (Thallus Eckloniae)
Extract active substances dry powder meter 33.900kg, extracting yield is 16.95%.(dry powder observed watercut is 4.9%).
Table 1 embodiment 1-3 product Contents of Main Components table
1. polysaccharide body (with glucose meter), phend-sulphuric acid.
2. protein (in terms of butt, n × 6.25), using gb5009-2010 standard detection.
3. content of iodine detection, using the mensure of sc/t 3010-2001 Thallus Laminariae (Thallus Eckloniae) content of iodine.
4. water soluble dietary fiber, using gb/t5009.88 standard determination method.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modify, all should belong to the covering scope of the present invention.
Claims (2)
1. a kind of production method of Thallus Laminariae (Thallus Eckloniae) extract it is characterised in that: comprise the following steps: Thallus Laminariae (Thallus Eckloniae) cleaning, pulverize, hydrolysis, enzyme
Solution, filtration, filtrate concentration, low temperature spray drying.
2. method according to claim 1 it is characterised in that: specifically comprise the following steps that
Step 1: Thallus Laminariae (Thallus Eckloniae) cleaning: remove soil, the impurity on Thallus Laminariae (Thallus Eckloniae) surface by cleaning;
Step 2: pulverize: the Thallus Laminariae (Thallus Eckloniae) cleaning up is pulverized, smashing fineness is 5-30 mesh;
Step 3: hydrolysis: by weight mixing Thallus Laminariae (Thallus Eckloniae) ground product with water for 1:10-15, stir, hydrolyze 2h;
Step 4: enzymolysis: percentage: add Digestive Enzyme 1.0%, proteinase-10 .5%, cellulose in Thallus Laminariae (Thallus Eckloniae) hydrolyzed solution
Enzyme 0.5%, pectase 1.0%, adjust ph=6-8,55-60 DEG C of enzymolysis 3-4h;Described lipase activity power is 1.5 × 105u/
Kg, proteinase activity power is 2 × 105U/kg, cellulose enzyme vigor is 10 × 105U/kg, pectase enzyme activity is 3 × 105u/
kg;Enzymolysis terminates, and when ph value is down to 4-5, feed liquid is warming up to 100 DEG C, and enzyme denaturing 2-3min obtains enzymolysis solution;
Step 5: filter: by enzymolysis solution first through colloid mill homogenizing, rotating speed 800-1000r/min is ground in rotation, then through high pressure homogenizer
Homogenizing, pressure 40-60mpa, finally filters, 200 keevil frames, filter pressure 20-30mpa, flow 0.5-1.0t/h, obtains filtrate;
Filtering residue is as agricultural fertilizer;
Step 6: filtrate concentrates: adopts dual-effect concentrator, evaporating temperature 55-70 DEG C, steam pressure 0.25mpa, filtrate is concentrated
To concentration brix=20-25%;
Step 7: low temperature spray drying: using vacuum and low temperature liquid continuous drier, temperature≤60-70 DEG C, evaporated quantity of water 65-
75kg/h, obtains Thallus Laminariae (Thallus Eckloniae) extract dry powder.
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Cited By (10)
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CN106957885A (en) * | 2017-05-19 | 2017-07-18 | 威海市四合生物科技有限公司 | A kind of zymotechnique of algal polysaccharides applied to aquaculture |
CN107319235A (en) * | 2017-07-28 | 2017-11-07 | 江苏南大耐雀生物技术有限公司 | A kind of fermented type sea-tangle solid beverage and preparation method thereof |
CN107467654A (en) * | 2017-07-17 | 2017-12-15 | 当代海洋生物科技(深圳)有限公司 | One main laminaria sea cucumber composite extract and its preparation method and application |
CN107474570A (en) * | 2017-08-28 | 2017-12-15 | 贵州省玉态康环保餐具有限公司 | A kind of degradable disponsable tableware and preparation method thereof |
CN107772413A (en) * | 2017-10-12 | 2018-03-09 | 天津春发生物科技集团有限公司 | A kind of natural sea band chop flavor Gly-His-Lys and preparation method thereof |
CN108295193A (en) * | 2018-01-18 | 2018-07-20 | 九仙尊霍山石斛股份有限公司 | A kind of intensive processing method and its application of Dendrobidium huoshanness |
CN109097403A (en) * | 2018-08-10 | 2018-12-28 | 青岛海莱美生物科技有限公司 | A kind of preparation method of brown alga extract |
CN113317463A (en) * | 2021-06-29 | 2021-08-31 | 威海市世代海洋生物科技股份有限公司 | Method for preparing kelp enzymolysis powder from kelp enzymolysis liquid |
CN114532500A (en) * | 2022-02-18 | 2022-05-27 | 福建农林大学 | Preparation method of active kelp matter |
CN116391761A (en) * | 2023-02-24 | 2023-07-07 | 安徽中志科技有限公司 | Selenium-enriched fruit and vegetable multi-vitamin nutrient protein solid beverage |
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Cited By (10)
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CN106957885A (en) * | 2017-05-19 | 2017-07-18 | 威海市四合生物科技有限公司 | A kind of zymotechnique of algal polysaccharides applied to aquaculture |
CN107467654A (en) * | 2017-07-17 | 2017-12-15 | 当代海洋生物科技(深圳)有限公司 | One main laminaria sea cucumber composite extract and its preparation method and application |
CN107319235A (en) * | 2017-07-28 | 2017-11-07 | 江苏南大耐雀生物技术有限公司 | A kind of fermented type sea-tangle solid beverage and preparation method thereof |
CN107474570A (en) * | 2017-08-28 | 2017-12-15 | 贵州省玉态康环保餐具有限公司 | A kind of degradable disponsable tableware and preparation method thereof |
CN107772413A (en) * | 2017-10-12 | 2018-03-09 | 天津春发生物科技集团有限公司 | A kind of natural sea band chop flavor Gly-His-Lys and preparation method thereof |
CN108295193A (en) * | 2018-01-18 | 2018-07-20 | 九仙尊霍山石斛股份有限公司 | A kind of intensive processing method and its application of Dendrobidium huoshanness |
CN109097403A (en) * | 2018-08-10 | 2018-12-28 | 青岛海莱美生物科技有限公司 | A kind of preparation method of brown alga extract |
CN113317463A (en) * | 2021-06-29 | 2021-08-31 | 威海市世代海洋生物科技股份有限公司 | Method for preparing kelp enzymolysis powder from kelp enzymolysis liquid |
CN114532500A (en) * | 2022-02-18 | 2022-05-27 | 福建农林大学 | Preparation method of active kelp matter |
CN116391761A (en) * | 2023-02-24 | 2023-07-07 | 安徽中志科技有限公司 | Selenium-enriched fruit and vegetable multi-vitamin nutrient protein solid beverage |
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