CN114532500A - Preparation method of active kelp matter - Google Patents
Preparation method of active kelp matter Download PDFInfo
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- CN114532500A CN114532500A CN202210150727.2A CN202210150727A CN114532500A CN 114532500 A CN114532500 A CN 114532500A CN 202210150727 A CN202210150727 A CN 202210150727A CN 114532500 A CN114532500 A CN 114532500A
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- kelp
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- 241000512259 Ascophyllum nodosum Species 0.000 title claims abstract description 59
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000013543 active substance Substances 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 11
- 238000001694 spray drying Methods 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims description 22
- 108090000790 Enzymes Proteins 0.000 claims description 15
- 102000004190 Enzymes Human genes 0.000 claims description 15
- 229940088598 enzyme Drugs 0.000 claims description 15
- 239000002699 waste material Substances 0.000 claims description 9
- 229920000615 alginic acid Polymers 0.000 claims description 8
- 235000010443 alginic acid Nutrition 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 6
- 108010061314 alpha-L-Fucosidase Proteins 0.000 claims description 5
- 102000012086 alpha-L-Fucosidase Human genes 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 108090000526 Papain Proteins 0.000 claims description 4
- 108010059820 Polygalacturonase Proteins 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 4
- 229940055729 papain Drugs 0.000 claims description 4
- 235000019834 papain Nutrition 0.000 claims description 4
- 108090000856 Lyases Proteins 0.000 claims description 3
- 102000004317 Lyases Human genes 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 108010004131 poly(beta-D-mannuronate) lyase Proteins 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 238000009210 therapy by ultrasound Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000013329 compounding Methods 0.000 claims description 2
- 230000009849 deactivation Effects 0.000 claims description 2
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 description 9
- 229920001543 Laminarin Polymers 0.000 description 9
- 239000005717 Laminarin Substances 0.000 description 9
- 238000000605 extraction Methods 0.000 description 8
- 230000008901 benefit Effects 0.000 description 7
- 150000004676 glycans Chemical class 0.000 description 7
- 229920001282 polysaccharide Polymers 0.000 description 7
- 239000005017 polysaccharide Substances 0.000 description 7
- 229920000855 Fucoidan Polymers 0.000 description 6
- 229920001542 oligosaccharide Polymers 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 229940072056 alginate Drugs 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- -1 alginate oligosaccharide Chemical class 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 150000002482 oligosaccharides Chemical class 0.000 description 4
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000002000 scavenging effect Effects 0.000 description 3
- 241001474374 Blennius Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 108010028144 alpha-Glucosidases Proteins 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000007086 side reaction Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 240000007817 Olea europaea Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- 238000007068 beta-elimination reaction Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Edible Seaweed (AREA)
Abstract
The invention discloses a preparation method of a kelp active substance, which is prepared by utilizing ultrasonic-assisted enzymolysis and spray drying methods. The invention provides a green preparation method of a kelp active substance, which is rapid, simple, high in yield, easy to obtain, low in cost and more suitable for large-scale production.
Description
Technical Field
The invention belongs to a production process of a kelp active substance enzymolysis method, and relates to a green preparation method of a kelp active substance, the active substance has high nutritive value and medicinal value, and the prepared kelp active substance has high biological activity, promotes the growth of plant roots, resists bacteria, improves the immunity of a human body, resists tumors, enhances physical strength and the like.
Background
The kelp is a macroalgae plant, is olive brown, has flat stripes, is rich in various nutrient components such as polysaccharide, amino acid, mineral substances, vitamins and the like, and has double effects of medicine and food. Laminarin, which is a main extract of kelp, has pharmacological actions of regulating blood fat, reducing blood sugar, resisting inflammation, oxidation, virus, tumor and the like, and the lipid-lowering efficacy of the laminarin has important significance for preventing and treating hyperlipidemia and complications thereof. Generally, the smaller the molecular weight of laminarin is, the higher the bioactivity of the laminarin is, the stronger the oxidation resistance of the laminarin with smaller molecular weight is, and the prepared laminarin active substance with small molecular weight solves the defects of complex molecular structure, poor absorption, difficult quality control and the like of the existing natural laminarin.
The fucoidan enzyme catalyzes and hydrolyzes high molecular weight fucoidan to obtain low molecular weight fucoidan, and the prepared low molecular weight polysaccharide or oligosaccharide has the advantages of good solubility, retention of the original biological activity of the polysaccharide, easier absorption and utilization and the like. Fucosidase can obtain series products with different quality grade specifications at different stages of oligosaccharide product separation and purification, and the application of the products in the market has good benefits for economy and medicine. The alginate lyase can be applied to the aspects of food, agriculture, pharmacy, energy and the like, can degrade alginate, can directionally prepare alginate oligosaccharide, and can also be used for clarifying the fine structure of the alginate, preparing seaweed protoplast, producing ethanol by fermentation, degrading a biological membrane and the like. The method combines enzymolysis, uses two specific enzymes and common complex enzyme to degrade active substances of herba Zosterae Marinae, and has the advantages of convenient operation, short extraction time, high efficiency, no pollution, and energy saving.
The kelp active substance is prepared by a green preparation method utilizing ultrasonic-assisted enzymolysis, and has the advantages of no side reaction, mild condition, environmental friendliness, energy consumption saving and the like. The kelp active substance with antioxidant activity prepared by ultrasonic-assisted enzymolysis in the invention has no other research reports or patents.
Disclosure of Invention
The invention aims to provide a green preparation method of a kelp active substance. The preparation process route is simple, reasonable, green and efficient, and is an effective method for preparing the active substances of the kelp. In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of active substances of kelp comprises the following steps:
(1) cleaning and drying the kelp waste raw material, and then crushing to 500 meshes of 360-fold sand by using a nano crusher; (2) adding distilled water with the weight of 10-20 times of that of the kelp powder into the kelp powder, soaking the kelp powder for 1-2 hours at normal temperature, carrying out ultrasonic treatment for 1-2 hours at normal temperature, adding a complex enzyme with the weight of 0.1-0.5% of the kelp powder, carrying out enzymolysis for 3-4 hours at 30-50 ℃, carrying out enzyme deactivation for 10 minutes at 100-120 ℃, and centrifuging to obtain a kelp active substance extracting solution, wherein the complex enzyme is prepared by compounding fucosidase, algin lyase, papain, cellulase and pectinase according to the mass ratio of 1: 1;
(3) and (3) carrying out spray drying on the kelp active substance extracting solution obtained in the step (2) by using a spray dryer to obtain kelp dry powder, namely the kelp active substance.
Further, in the step (1), the kelp waste raw material comprises all available kelp such as kelp roots and stems and the remaining kelp waste damaged in the processing production.
Further, in the step (2), all conditions in the processing technology are green and normal temperature, and all used reagents are non-toxic and safe reagents.
Further, in the step (2), the fucoidan enzyme catalyzes and hydrolyzes the high molecular weight fucoidan to obtain the low molecular weight fucoidan, and the prepared low molecular weight polysaccharide or oligosaccharide has the advantages of good solubility, retention of the original biological activity of the polysaccharide, easier absorption and utilization and the like.
Further, in the step (2), the alginate lyase degrades the alginate into alginate oligosaccharides through beta-elimination to improve the absorption capacity of active substances.
Furthermore, in the step (2), the complex enzyme extraction mode can fully destroy the plant cell structure aiming at the composition and the structure of the plant cell wall, the condition is mild, and the structure and the activity of the active substance can be effectively protected while the extraction rate of the laminarin is obviously improved.
Further, in the step (3), the kelp active substance obtained by the spray drying technology has uniform powder quality, bright luster and physical and chemical indexes and microbial indexes meeting the national standard requirements; and the spray drying is used for preparing the powder, the equipment price is low, the operation is simple and convenient, and meanwhile, the continuous large-scale production can be carried out, so that the method is suitable for large-scale industrial production.
The invention has the advantages that:
(1) the extraction method is reasonable and effective, and the green preparation method using ultrasonic-assisted enzymolysis has the advantages of no side reaction, mild condition, environmental friendliness, energy consumption saving and the like;
(2) the invention purchases the kelp waste for extraction, and greatly utilizes the kelp resource;
(3) all conditions of the method are green and normal temperature, and the used reagents are nontoxic and safe reagents, so that the method is an environment-friendly extraction method;
(4) all the complex enzyme extraction modes of the invention can fully destroy the plant cell structure aiming at the plant cell wall composition and structure, the condition is mild, and the structure and activity of active substances can be effectively protected while the extraction rate of laminarin is obviously improved. The prepared low molecular weight polysaccharide or oligosaccharide not only has good solubility, but also retains the original biological activity of the polysaccharide, is easier to absorb and utilize, and the like.
(5) The obtained active substance powder of herba Zosterae Marinae is convenient for storage and carrying, and can be used as a product alone, or further processed into capsule, granule, etc., and supplemented with other foods to develop various functional foods, so as to make the health food more diversified in functionality.
Detailed Description
The present invention will be described in further detail below by way of examples, but the present invention is not limited to the following examples.
Example 1
A green preparation method of active substances of kelp comprises the following steps:
(1) cleaning and drying kelp roots which are used as raw materials of kelp wastes, and then crushing the kelp roots into 500 meshes by using a nano crusher; (2) adding distilled water with the weight of 20 times of the kelp powder, soaking for 2 hours at normal temperature, carrying out ultrasonic treatment for 2 hours at normal temperature, adding complex enzyme (fucosidase, algin lyase, papain, cellulase and pectinase are compounded according to the mass ratio of 1: 1) with the weight of 0.1% of the kelp powder, carrying out complex enzymolysis for 4 hours at the temperature of 35 ℃, inactivating the enzyme at the temperature of 100 ℃ for 10 minutes, and centrifuging to obtain the active substance extracting solution of the kelp.
(3) And (3) carrying out spray drying on the kelp active substance extracting solution obtained in the step (2) by using a spray dryer to obtain kelp dry powder, namely the kelp active substance.
(4) The yield of the prepared kelp active substance dry powder is 36 percent, the kelp active substance dry powder is light brown powder, and the DPPH, scavenging capacity, ABTS, scavenging capacity, OH scavenging capacity, alpha-glucosidase inhibition rate and other anti-oxidation and hypoglycemic activity indexes of the kelp active substance dry powder with different concentrations are measured.
The results are as follows:
removal rate of DPPH radical (%)
Removal rate of ABTS free radical (%)
Removal ratio (%) of kelp active substance dry powder to. OH
Inhibition ratio (%) of kelp active substance dry powder to alpha-glucosidase
The above description is only a preferred embodiment of the present invention, and all the equivalent changes and modifications made according to the claims of the present invention should be covered by the present invention.
Claims (6)
1. A preparation method of a kelp active substance is characterized by comprising the following steps:
(1) cleaning and drying the kelp waste raw material, and then crushing the kelp waste raw material by using a nano crusher; (2) adding distilled water with the weight of 10-20 times of that of the kelp powder into the kelp powder, soaking the kelp powder for 1-2 hours at normal temperature, carrying out ultrasonic treatment for 1-2 hours at normal temperature, adding complex enzyme with the weight of 0.1-0.5% of the kelp powder, carrying out enzymolysis for 3-4 hours at 30-50 ℃, carrying out enzyme deactivation for 10 minutes at 100-120 ℃, and centrifuging to obtain a kelp active substance extracting solution, wherein the complex enzyme consists of fucosidase, algin lyase, papain, cellulase and pectinase;
(3) and (3) carrying out spray drying on the kelp active substance extracting solution obtained in the step (2) by using a spray dryer to obtain kelp dry powder, namely the kelp active substance.
2. The method according to claim 1, wherein in the step (1), the kelp waste raw material comprises kelp roots, stems and kelp waste remaining after breakage in processing.
3. The method as claimed in claim 1, wherein in the step (1), the nano-pulverizer pulverizes to 360-500 mesh.
4. The preparation method of claim 1, wherein the complex enzyme is prepared by compounding fucosidase, alginate lyase, papain, cellulase and pectinase at a mass ratio of 1: 1.
5. The method according to claim 1, wherein the enzymolysis temperature in the step (2) is 35 ℃.
6. The method according to claim 1, wherein the enzymolysis time in step (2) is 4 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210150727.2A CN114532500A (en) | 2022-02-18 | 2022-02-18 | Preparation method of active kelp matter |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210150727.2A CN114532500A (en) | 2022-02-18 | 2022-02-18 | Preparation method of active kelp matter |
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| CN114532500A true CN114532500A (en) | 2022-05-27 |
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| CN202210150727.2A Pending CN114532500A (en) | 2022-02-18 | 2022-02-18 | Preparation method of active kelp matter |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515592A (en) * | 2003-01-02 | 2004-07-28 | 合肥学院 | Process for preparing low molecular weight fucidan by using microbial enzyme method |
| CN103504368A (en) * | 2013-09-18 | 2014-01-15 | 中国科学院天津工业生物技术研究所 | Kelp-flavored food ingredient and preparation method thereof |
| CN106343410A (en) * | 2016-09-12 | 2017-01-25 | 福建天斯顿生物科技有限公司 | Production method of kelp extract |
| CN106538991A (en) * | 2016-11-01 | 2017-03-29 | 福建农林大学 | A kind of method that ultrasonic acidolysis pre-treat biological prepares kelp blood pressure product |
| CN107312807A (en) * | 2017-06-20 | 2017-11-03 | 福州大学 | The enzymolysis preparation of the brown alga function oligosaccharides in one main laminaria source |
| CN110301601A (en) * | 2019-07-24 | 2019-10-08 | 山东海之宝海洋科技有限公司 | A kind of soluble Kelp Powder and its Production by Enzymes method containing brown alga oligose |
-
2022
- 2022-02-18 CN CN202210150727.2A patent/CN114532500A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515592A (en) * | 2003-01-02 | 2004-07-28 | 合肥学院 | Process for preparing low molecular weight fucidan by using microbial enzyme method |
| CN103504368A (en) * | 2013-09-18 | 2014-01-15 | 中国科学院天津工业生物技术研究所 | Kelp-flavored food ingredient and preparation method thereof |
| CN106343410A (en) * | 2016-09-12 | 2017-01-25 | 福建天斯顿生物科技有限公司 | Production method of kelp extract |
| CN106538991A (en) * | 2016-11-01 | 2017-03-29 | 福建农林大学 | A kind of method that ultrasonic acidolysis pre-treat biological prepares kelp blood pressure product |
| CN107312807A (en) * | 2017-06-20 | 2017-11-03 | 福州大学 | The enzymolysis preparation of the brown alga function oligosaccharides in one main laminaria source |
| CN110301601A (en) * | 2019-07-24 | 2019-10-08 | 山东海之宝海洋科技有限公司 | A kind of soluble Kelp Powder and its Production by Enzymes method containing brown alga oligose |
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