CN108084290A - A kind of method of polysaccharide in microwave radiation exaraction common vetch dish - Google Patents
A kind of method of polysaccharide in microwave radiation exaraction common vetch dish Download PDFInfo
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- CN108084290A CN108084290A CN201810017341.8A CN201810017341A CN108084290A CN 108084290 A CN108084290 A CN 108084290A CN 201810017341 A CN201810017341 A CN 201810017341A CN 108084290 A CN108084290 A CN 108084290A
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- common vetch
- vetch dish
- polysaccharide
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- microwave radiation
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a kind of method of polysaccharide in microwave radiation exaraction common vetch dish, the extractive technique fields of category agricultural product active ingredient.It is concretely comprised the following steps：Fresh common vetch dish is cleaned, 80 DEG C of dryings crush to constant weight and cross 60 mesh sieves, methanol eddy degreasing 3 times, filter residue and drying is to constant weight after suction filtration, add appropriate amount of water, aid in treatment 1.5min under microwave 240w repeats extraction 3 times, stirring filters and concentrated supernatant, filtering will be precipitated by adding in 95% ethyl alcohol of three times, and vacuum freeze drying precipitates after centrifugation, obtains common vetch dish Thick many candies powder；Common vetch dish holosaccharide product is obtained using de- albumen, decoloration, macroreticular resin, dialysis purification, freeze-drying；The coarse polysaccharide extractive rate that the present invention is extracted from common vetch dish is 21.96%, and holosaccharide recovery rate is 3.08%, easy to operate, and recovery rate is high, and purifying is fine, good product quality.
Description
Technical field
A kind of method of polysaccharide in microwave radiation exaraction common vetch dish belongs to the extractive technique field of agricultural product active ingredient, tool
Body is related to a kind for the treatment of technology of Microwave Extraction polysaccharide, and final coarse polysaccharide extractive rate is 21.96%, and holosaccharide recovery rate is
3.08%, provide foundation for the extracting method of polysaccharide compound.
Background technology
Common vetch dish is Osmundaceae plant, and this patent refers to northeast production common vetch dish.The delicious few fiber of common vetch dish, has very high battalion
Support value, healthcare function and medical value, containing abundant vitamin, protein, amino acid, polysaccharide, flavones, fat, acid of trampling on,
A variety of needed by human body nutritional ingredients such as inorganic salts.Common vetch dish and its main component have antitumor and anticancer, anti-oxidant and anti-aging,
The effects that antibacterial, anti-inflammatory, and have clearing heat and detoxicating, moistening lung qi-regulating, qi-restoratives relax network, hemostasis, tranquilizing the mind, decompression, it is antipyretic the effect of, it is most main
The ingredient wanted is polysaccharide and flavone compound, is a kind of medicine-food two-purpose wild vegetables of great exploitation potential.
Polysaccharide is the indispensable component of life entity, and existing form has starch, glycogen, cellulose, synanthrin, chitin
Matter, agar, glutinous polysaccharide etc..Polysaccharide have the function of certain bioactivity and, it is nontoxic to normal cell, and in Chinese medicine polysaccharide tool
Have that adjust functions, the medical values such as immune, hypoglycemic, antitumor, treatment, Guan Rong, emulsification very big.Polysaccharide is by multiple monose point
Son condensation, dehydration forms, and is the complicated and huge glucide of molecule, extremely wide in distributed in nature.In plant
Polysaccharide has the effects that improve immunity of organisms, antibacterial, anti-mutagenesis and antitumor.Osmunda polysaccharides have apparent bacteriostatic activity and
Bacteriostasis is broad spectrum activity.
Mainly there are alkaline extraction, sour formulation, salt formulation and Hot water extraction etc. for the extracting method of polysaccharide compound.Acid
Formulation and alkaline extraction easily make polysaccharide that glycosidic bond fracture occur, and par-tial polysaccharide hydrolyzes and reduces Polyose extraction.Water extract method generally consumes
Duration, high energy consumption, extraction solvent usage amount is big, polysaccharide extract rate is low.Enzyme price general charged in enzyme process assisted extraction is expensive, often
Also there is the shortcomings of easily inactivation, short life, purity is inadequate.It is compared with traditional heating extraction, Microwave Extraction has quality height, production
Measure the advantages of big, easy to operate, extraction is fast, less energy consumption, few, time saving solvent dosage, can effectively protect food, drug and its
Functional component in his chemical material belongs to safety non-pollution green engineering.Electromagnetic field caused by microwave can accelerate to be extracted
The rate that the molecule of component is spread from solid interior to solid liquid interface.Microwave field is mainly derived from the booster action of Polyose extraction
Microwave is to the biological effect of cell membrane：Raise cell interior temperature, pressure increase, when pressure is more than the ability to bear of cell membrane
When, cell wall rupture, the polysaccharide positioned at cell interior is released from cell, is dissolved by the solvent of surrounding, therefore its heat
It is consistent to transfer with the direction of mass transfer, this contributes to the Fast Stripping of polysaccharide.
People have successfully extracted polysaccharide from nearly hundred kinds of plants and mushroom, such as lentinan, lily polysaccharide, Chinese yam
Polysaccharide, Tea Polysaccharides etc., osmunda polysaccharides extraction conditions are optimized in the present invention, and osmunda polysaccharides are purified, and are
The further investigation of osmunda polysaccharides provides the foundation.Using polysaccharide in microwave radiation exaraction common vetch dish one kind is made in product by the present invention
New food additives and antistaling agent, functional character is strengthened and safe green is pollution-free.
The content of the invention
Technical problem：Sour formulation and alkaline extraction easily make polysaccharide that glycosidic bond fracture occur, and par-tial polysaccharide hydrolyzes and reduces polysaccharide
Extraction.Generally time-consuming for water extract method, and high energy consumption, extraction solvent usage amount is big, polysaccharide extract rate is low.In enzyme process assisted extraction
Enzyme price general charged is expensive, there is the shortcomings of easily inactivation, short life, purity is inadequate toward contact.
The purpose of the present invention：It is for the low present situation of the disadvantages mentioned above during Polyose extraction and recovery rate, exploitation one kind
The method of polysaccharide in microwave radiation exaraction common vetch dish, this method is not only simple to equipment requirement, shortens extraction time, improves polysaccharide
Recovery rate, purifying products are fine, best in quality, and safe green is pollution-free.
Technical solution：The method of polysaccharide, specifically comprises the following steps in a kind of microwave radiation exaraction common vetch dish：
(1) pretreatment of raw material：Fresh common vetch dish is cleaned, 80 DEG C of drying to constant weights be put into universal high speed disintegrator crushing, pass through
It is spare that 60 mesh sub-sieves obtain common vetch vegetable powder；
(2) microwave radiation technology is handled：Common vetch vegetable powder and distilled water according to select solid-to-liquid ratio for:1:20,1:30,1:40,1:50,1:60
Ratio mixes, and places in triangular flask, and 1.5min is extracted under conditions of microwave power 240w, extracts 3 times, is spaced 5min；
(3) extraction of Thick many candies：Stirring filters and concentrated supernatant, adds in 95% ethyl alcohol of three times and filters precipitation, centrifugation
Vacuum freeze drying precipitates after 7000rpm, 3min, obtains common vetch dish Thick many candies powder；
(4) purify：Thick many candies obtained by step (3) are pressed 1:50 rehydrations into Thick many candies solution, then through Sevage methods removing protein, in mistake
Hydrogen oxide addition is 30%, and bleaching temperature is to decolourize under conditions of 60 DEG C of bleaching times are 2h, D101 macroreticular resins after activation
Holosaccharide product is obtained after the operations such as purifying, dialysis and freeze-drying.
Beneficial effects of the present invention：
1. the extraction raw material that the present invention takes is the wild vegetables of integration of drinking and medicinal herbs, inherently there is abundant nutrition and functional object
Matter, the methods of common soda acid, acetone equal solvent and ultrasonic extraction are substituted using microwave radiation exaraction system, equipment requirement letter
It is single, recovery rate is improved, production cost is reduced, while avoids environmental pollution.
2. the pretreatment of raw material in the present invention, is crushed using high speed disintegrator, obtained spare common vetch vegetable powder, can be better
Convenient for the extraction of follow-up polysaccharide, extraction efficiency is improved indirectly.
3. using microwave radiation exaraction, extraction time is substantially reduced compared with traditional water extraction, long-time heating can be avoided, broken
To the polysaccharide component that long-time heating is sensitive and is denatured in bad common vetch dish, extraction efficiency is big, and final coarse polysaccharide extractive rate is
21.96%, more traditional water extract method improves 3.6 times, and the time shortens 10 times；The more single cellulase extraction of recovery rate carries
1.5 times high, the time shortens 1.8 times.The polysaccharide extracting process route that method carries out accordingly farthest maintains polysaccharide
Functional component, purity is high, and pollution is few, at low cost, high efficiency.
Specific embodiment
With reference to specific embodiment, the present invention is furture elucidated, present embodiment using technical solution of the present invention as
Under the premise of implemented, it should be understood that these modes are only illustrative of the invention and is not intended to limit the scope of the invention.
Embodiment 1
Fresh common vetch dish is cleaned, 80 DEG C of dryings to constant weight, universal high speed disintegrator is put into after drying and crushes and crosses 60 mesh sieves, methanol
Flow back degreasing 3 times, and it is spare to obtain common vetch vegetable powder to constant weight for filter residue and drying after suction filtration；5g common vetch vegetable powders is taken to be positioned in triangular flask, by 1:50
Ratio add water, aid in treatment 1.5min under microwave 240w repeats extraction 3 times, and stirring filters and concentrated supernatant, adds in three times
The precipitation filtering overnight of 95% ethyl alcohol, vacuum freeze drying precipitates after centrifuging 7000rpm, 3min, obtains common vetch dish Thick many candies powder；It is true afterwards
Vacuum freecing-dry precipitates, and obtains common vetch dish Thick many candies powder.Gained Thick many candies press 1:50 rehydrations are into Thick many candies solution, then through Sevage methods
Removing protein is 30% twice, in hydrogen peroxide addition, and bleaching temperature is to decolourize under conditions of 60 DEG C of bleaching times are 2h, activation
Afterwards D101 macroporous resin purifications, dialysis, after the operations such as alcohol precipitation and freeze-drying obtain holosaccharide product again.After testing, this side
Coarse polysaccharide extractive rate made from method is 21.96%, and holosaccharide recovery rate is 3.08%.
Embodiment 2
Fresh common vetch dish is cleaned, 80 DEG C of dryings to constant weight, and universal high speed disintegrator is put into after drying and crushes and crosses 60 mesh sieves, and methanol returns
Degreasing 3 times is flowed, it is spare to obtain common vetch vegetable powder to constant weight for filter residue and drying after suction filtration；5g common vetch vegetable powders is taken to be positioned in triangular flask, by 1:30
Ratio adds water, aid in treatment 1.5min under microwave 240w, repeats extraction 3 times, and stirring filters and concentrated supernatant, adds in three times
The precipitation filtering overnight of 95% ethyl alcohol, vacuum freeze drying precipitates after centrifuging 7000rpm, 3min, obtains common vetch dish Thick many candies powder；It is true afterwards
Vacuum freecing-dry precipitates, and obtains common vetch dish Thick many candies powder.Gained Thick many candies press 1:50 rehydrations are into Thick many candies solution, then through Sevage methods
Removing protein is 30% twice, in hydrogen peroxide addition, and bleaching temperature is to decolourize under conditions of 60 DEG C of bleaching times are 2h, activation
Afterwards D101 macroporous resin purifications, dialysis, after the operations such as alcohol precipitation and freeze-drying obtain holosaccharide product again.After testing, this side
Coarse polysaccharide extractive rate made from method is 20.34%, and holosaccharide recovery rate is 2.89%.
Embodiment 3
Fresh common vetch dish is cleaned, 80 DEG C of dryings to constant weight, universal high speed disintegrator is put into after drying and crushes and crosses 60 mesh sieves, methanol
Flow back degreasing 2 times, and it is spare to obtain common vetch vegetable powder to constant weight for filter residue and drying after suction filtration；5g common vetch vegetable powders is taken to be positioned in triangular flask, by 1:50
Ratio add water, aid in treatment 2min under microwave 240w repeats extraction 3 times, and stirring filters and concentrated supernatant, adds in three times
The precipitation filtering overnight of 95% ethyl alcohol, vacuum freeze drying precipitates after centrifuging 7000rpm, 3min, obtains common vetch dish Thick many candies powder；It is true afterwards
Vacuum freecing-dry precipitates, and obtains common vetch dish Thick many candies powder.Gained Thick many candies press 1:30 rehydrations are into Thick many candies solution, then through Sevage methods
Removing protein is 40% twice, in hydrogen peroxide addition, and bleaching temperature is to decolourize under conditions of 60 DEG C of bleaching times are 2h, activation
Afterwards D101 macroporous resin purifications, dialysis, after the operations such as alcohol precipitation and freeze-drying obtain holosaccharide product again.After testing, this side
Coarse polysaccharide extractive rate made from method is 22.54%, and holosaccharide recovery rate is 2.78%.
Two kinds of embodiments described above are for only for ease of the example for understanding and lifting, it is noted that for this technology
For the technical staff in field, without departing from the principle of the present invention, can technique according to the invention scheme and its preferably
Several improvement are made in the description of embodiment, these improvement should also belong to the scope of protection of the present invention.
Claims (4)
1. a kind of method of polysaccharide in microwave radiation exaraction common vetch dish, feature is methanol eddy degreasing after common vetch dish dries pulverizing
3 times, filter residue and drying adds appropriate amount of water, first progress microwave radiation technology processing, aid in treatment under microwave 240w to constant weight after suction filtration
1.5min repeats extraction 3 times, stirs and filters simultaneously concentrated supernatant, and filtering, vacuum after centrifugation will be precipitated by adding in 95% ethyl alcohol of three times
Freeze-drying precipitation, obtains common vetch dish Thick many candies powder；Using de- albumen, decoloration, macroreticular resin, dialysis purification, it is freeze-dried
To common vetch dish holosaccharide product；Final coarse polysaccharide extractive rate is 21.96%, and holosaccharide recovery rate is 3.08%.
2. according to claim 1 in common vetch dish polysaccharide extraction process, which is characterized in that specifically comprise the following steps：
(1) pretreatment of raw material：Fresh common vetch dish is cleaned, 80 DEG C of drying to constant weights be put into universal high speed disintegrator crushing, pass through
It is spare that 60 mesh sub-sieves obtain common vetch vegetable powder；
(2) microwave radiation technology is handled：Common vetch vegetable powder and distilled water according to select solid-to-liquid ratio for:1:20,1:30,1:40,1:50,1:60
Ratio mixes, and places in triangular flask, and 1.5min is extracted under conditions of microwave power 240w, extracts 3 times；
(3) extraction of Thick many candies：Stirring filters and concentrated supernatant, adds in 95% ethyl alcohol of three times and filters precipitation, true after centrifugation
Vacuum freecing-dry precipitates, and obtains common vetch dish Thick many candies powder；
(4) purify：Thick many candies obtained by step (3) are pressed 1:50 rehydrations into Thick many candies solution, then through Sevage methods removing protein, in mistake
Hydrogen oxide addition is 30%, and bleaching temperature is to decolourize under conditions of 60 DEG C of bleaching times are 2h, D101 macroreticular resins after activation
Holosaccharide product is obtained after the operations such as purifying, dialysis and freeze-drying.
3. according to claim 1 or claim 2, a kind of method of polysaccharide in microwave radiation exaraction common vetch dish, which is characterized in that microwave carries
The time interval taken is 5min.
4. according to claim 1 or claim 2, a kind of method of polysaccharide in microwave radiation exaraction common vetch dish, which is characterized in that common vetch dish is more
Sugared extracting solution centrifugal condition is:Rotating speed 7000rpm, centrifugation time 3min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110693025A (en) * | 2019-11-01 | 2020-01-17 | 东北林业大学 | Multifunctional component synergistic blood sugar reducing composition and application thereof |
| CN115428830A (en) * | 2022-08-18 | 2022-12-06 | 盐城工学院 | Method for promoting self-healing of mechanically damaged fruits based on functional sugar self-assembly membrane and application of method |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4886666A (en) * | 1987-01-27 | 1989-12-12 | Yaguang Liu | Pharmaceutical composition for inhibiting viruses and increasing immune function (PII) |
| CN106794211A (en) * | 2014-08-18 | 2017-05-31 | 制药创源有限公司 | Polygalacturonase rhamnosan galacturonic acid (PGRG1) composition |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110693025A (en) * | 2019-11-01 | 2020-01-17 | 东北林业大学 | Multifunctional component synergistic blood sugar reducing composition and application thereof |
| CN115428830A (en) * | 2022-08-18 | 2022-12-06 | 盐城工学院 | Method for promoting self-healing of mechanically damaged fruits based on functional sugar self-assembly membrane and application of method |
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