CN101743854B - Method for preparing morchella mycelium by using dendrobium fiber as substrate - Google Patents
Method for preparing morchella mycelium by using dendrobium fiber as substrate Download PDFInfo
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- CN101743854B CN101743854B CN 200910155278 CN200910155278A CN101743854B CN 101743854 B CN101743854 B CN 101743854B CN 200910155278 CN200910155278 CN 200910155278 CN 200910155278 A CN200910155278 A CN 200910155278A CN 101743854 B CN101743854 B CN 101743854B
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Abstract
The invention provides a method for preparing morchella mycelium by using dendrobium fiber as substrate. The preparation method adopts solid culture medium for culture. The solid culture medium contains the following formula components by mass, preferably, 70-90 percent of cellulose residue obtained from crushed dendrobium candidum after extract is extracted, 5-20 percent of corn, 0.5-1 percent of sodium selenite, 0.5-1 percent of germanium dioxide, 0.01-0.2 percent of potassium dihydrogen phosphate and 50-60 percent of water. The pH value is natural. The dendrobium candidum cellulose in the solid culture medium is fluffy and porous, can facilitate the growth of morchella aerial mycelium and can improve the yield of morchella mycelium. Since the culture medium contains elements such as inorganic selenium, germanium and the like, the content of the organic germanium and the inorganic selenium in morchella mycelium is improved. The invention has the advantages that the entire culture medium is edible and can be directly used in healthcare products and food raw materials, the invented technology is mature and stable, the yield of morchella mycelium is high, the production cycle is short and the production cost is greatly reduced.
Description
Technical field
The invention belongs to technical field of bioengineering.The method that relates to specifically a kind of Morchella esculenta (L.) Pers mycelium take the dendrobium fiber element as matrix.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo) claims again to be the orchid family Dendrobium medicinal plant by iron bracketplant, and its dry product is Tiepi Fengdou.Dendrobium candidum is a kind of famous and precious Valuable Herbal Medicine in imminent danger, has except gas under the fraud, and tonifying five zang organs labor is won thin, and reinforcing yin essence is grown the effect of essence, is widely used in clinical and health products.In the preparation process of dendrobium candidum medicinal extract, the dregs of a decoction of dendrobium candidum after water extraction are generally all thrown away, and it is mainly cellulose, contain more a small amount of active components that are not extracted, and are edible.This has wasted the availability of this rare medicinal material in imminent danger greatly.
Hickory chick (morechella.spp) is the famous food of ascus bacterial classification, the dual-purpose rare fungus resource of medicine, belong to Ascomycotina, discomycete, Pezizale, saddle Cordycepps, morchella (morechella) in the classification, its fruit body and mycelium are famous because being rich in delicate flavour material.The mycelium of hickory chick contains abundant amino acid and other mineral nutrient element, particularly essential amino acid content is higher, the content of glutamic acid, lysine, aspartic acid that wherein determines delicate flavour and trophic function is particularly outstanding, also contain cystine, lysine, arginine and can promote children growth and intelligence, branched-chain amino acid is conducive to the control of multiple hepatopathy.Fermented morel liquid and mycelium zoopery show, the hickory chick granulose that its mycelium kind contains, selenium element, organic germanium play an important role in improving animal immune, antitumor process.
The artificial cultivation lab scale idol of hickory chick has successful report, but the poor repeatability of experiment in cultivation still can not be carried out the commercialization cultivation so far, and the hickory chick on the market is all from wild.Separation, purifying directly apply to cultivation or the mycelium production of hickory chick.The hickory chick spore germination forms mycelia, and Morciiella Esculeuta Mycelia constantly decomposes, and absorbs nutrient, has just formed the hickory chick entity when other condition such as suitable illumination, air, temperature cooperates.The needed constitute of Morchella esculenta (L.) Pers sporophore has nutriment over half to derive from storage nutrient in the mycelium, so mycelial nutrient content can be than high times of fruit body on general.
Adopt ripe fungal culture technology, produce in a large number mycelium, exploitation wild toadstool mycelium product is one of most economical effective approach that solves the hickory chick imbalance between supply and demand.In view of above-mentioned factor, develop the plain Morchella esculenta (L.) Pers mycelium of a kind of dendrobium fiber edible, that medicine is dual-purpose, significant.
Summary of the invention
But the preparation method of the purpose of this invention is to provide a kind of medicine, eating dual-purpose dendrobium candidum cellulose Morchella esculenta (L.) Pers mycelium.
The present invention is to realize by such technical scheme in order to achieve the above object.A kind of solid culture method of dendrobium candidum cellulose Morchella esculenta (L.) Pers mycelium is provided.The suitableeest mass fraction of each recipe ingredient of this solid culture medium is:
Cellulose dregs 70%-90%, corn 5%-20%, adding 0.5%-1% sodium selenite, 0.5%-1% germanium dioxide, potassium dihydrogen phosphate 0.01%-0.2%, water content 50%-60%, the pH value nature of dendrobium candidum after medicinal extract is extracted in ultra-fine grinding.
Stem of noble dendrobium method for preparing morchella mycelium of the present invention is realized by following steps:
(1) get corn, corn flour is broken into grain of rice size eluriates totally to be placed in the clear water and soak, then filtering element (cartridge) water is put into the steamer Steam by water bath to the white heart of mistake.
(2) get offcuts and the middle abundant mixing of material of step (1) that dendrobium candidum extracts the rear dregs of a decoction.
(3) get potassium dihydrogen phosphate, sodium selenite, germanium dioxide, add the pure water of an amount of ratio, regulate the water content of medium.Pour the material that is mixed in the step (2) into, stir.
(4) medium in the step (3) carries out sterilization after the bottling, naturally takes out inoculation hickory chick bacterial classification after the cooling.
(5) optimization of condition of culture: under the fixing prerequisite of medium, the Morciiella Esculeuta Mycelia growth is mainly relevant with temperature, incubation time, these three conditions of humidity.In order to obtain better suitable dendrobium candidum cellulose Morchella esculenta (L.) Pers mycelium growth conditions, design L
9(3
4) orthogonal test table.Each factor is established 3 addition levels.Finally optimize the growth conditions of best suitable dendrobium fiber element Morciiella Esculeuta Mycelia.
(6) under the condition of culture of optimization, cultivate.Cover with behind the mycelia dendrobium candidum cellulose Morchella esculenta (L.) Pers mycelium.
Preferably, iblet requirement in the described step (1), nothing is gone mouldy, the high-grade maize of the bad grain of nothing.Soak time is 24 hours after pulverizing.
Preferably, the offcuts of the dregs of a decoction after the processing of described step (2) dendrobium candidum make it fluffy after the drying.
Preferably, described step (3) potassium dihydrogen phosphate, sodium selenite and germanium dioxide dissolve with 45 ℃ of warm water, and water dilutes is convenient to stir controlled humidity.
Preferably, the method for described step (4) sterilization adopts high pressure steam sterilization, and its condition is: under 0.14Mpa, keep 2h under 126 ℃.Naturally cooling refers to be cooled to below 30 ℃, inoculates
Preferably, described step (5) is carried out the preferred of condition of culture, and obtain best condition of culture and be: temperature is 24 ℃, and humidity 55% when incubation time is 20 days, is the optimal conditions of mycelia preparation.
Preferably, the condition of described step (6) cultivation should be cultivated under dark condition.
Advantage of the present invention: the dendrobium candidum cellulose has the characteristics of bulk multi-hole gap in (1) solid culture medium of the present invention, is conducive to the growth of hickory chick aerial hyphae, has improved the output capacity of Morchella esculenta (L.) Pers mycelium.The enriching yin that dendrobium fiber has, the effect that improves body immunity also can demonstrate fully out in this product.(2) Morchella esculenta (L.) Pers mycelium of the present invention is nutritious, and mycelia yield is high.(3) be rich in selenium and element ge in the medium of the present invention, the Morchella esculenta (L.) Pers mycelium after growing up to is rich in Organic Selenium and organic germanium, makes Morchella esculenta (L.) Pers mycelium raising body immunity and antineoplastic action more obvious.(4) active ingredient Morchella esculenta (L.) Pers polysaccharide of the present invention, delicate flavour amino acid, mineral matter element etc. are suitable with natural Morchella esculenta (L.) Pers sporophore.Can be used as the substitute of Morchella esculenta (L.) Pers sporophore fully.Alleviated the imbalance between supply and demand of this unrealized tame rare bacterium of hickory chick.And medium of the present invention, whole edible.Can be directly used in food materials and health products raw material.(5) technical maturity of the present invention, Morchella esculenta (L.) Pers mycelium output is high, and is with short production cycle, and production technology is simple, greatly reduces production cost.
Embodiment
In order to make those skilled in the art person understand better the present invention program, the preparation method to dendrobium candidum cellulose Morciiella Esculeuta Mycelia of the present invention is described in further detail below in conjunction with embodiment.
Embodiment 1
Dendrobium candidum cellulose method for preparing morchella mycelium is made by following step:
(1) by each nutriment of prescription weighing
The dry dregs of a decoction after dendrobium candidum extracts (extraction is offcuts afterwards, generally abandon to be rubbish) 70%, corn 20%, 0.5% sodium selenite, 0.5% germanium dioxide potassium dihydrogen phosphate 0.1%, water content 50%, pH value nature.
(2) get corn (without going mouldy, without bad grain), corn flour be broken into grain of rice size eluriate totally to be placed in the clear water and soak, filtering element (cartridge) water then, put into the steamer Steam by water bath to the white heart, pull out after draining away the water.
(3) auxiliary material potassium dihydrogen phosphate, sodium selenite and germanium dioxide are made it in 45 ℃ of warm water, dissolve, the dilution certain multiple.Mix thoroughly with iblet and cellulose.
(4) vexed 1-2 hour (day-light saving time should not be long) bottles after material fully suctions water, feeds to shoulder, tightens lower pine and flattens charge level, cleans bottleneck, sealing.
(5) dress pot sterilization autoclaving is kept and was taken the dish out of the pot in 2 hours in 126 ℃, pressure position 0.14Mpa, is cooled to below 30 ℃ and waits to inoculate.
(6) with postvaccinal bacterial classification, and cultivate under the black chamber.
Above-mentioned steps prepares in the method for dendrobium candidum cellulose Morchella esculenta (L.) Pers mycelium,
Embodiment 2
Dendrobium candidum cellulose method for preparing morchella mycelium is made by following step:
(1) by each nutriment of prescription weighing
The dry dregs of a decoction after dendrobium candidum extracts (offcuts after extracting, generally abandon be rubbish) 80%, corn 10%, add 1% sodium selenite, 1% germanium dioxide, potassium dihydrogen phosphate 0.1%, water content 55%, pH value nature.
(2) get corn (without going mouldy, without bad grain), corn flour be broken into grain of rice size eluriate totally to be placed in the clear water and soak, filtering element (cartridge) water then, put into the steamer Steam by water bath to the white heart, pull out after draining away the water.
(3) auxiliary material potassium dihydrogen phosphate, sodium selenite and germanium dioxide are made its fully dissolving in 45 ℃ of warm water, dilution certain multiple.Mix thoroughly with iblet and cellulose.
(4) vexed 1-2 hour (day-light saving time should not be long) bottles after material fully suctions water, feeds to shoulder, tightens lower pine and flattens charge level, cleans bottleneck, sealing.
(5) dress pot sterilization autoclaving is kept and was taken the dish out of the pot in 2 hours in 126 ℃, pressure position 0.14Mpa, is cooled to below 30 ℃ and waits to inoculate.
(6) with postvaccinal bacterial classification, and cultivate under the black chamber.
Above-mentioned steps prepares in the method for dendrobium candidum cellulose Morchella esculenta (L.) Pers mycelium,
Embodiment 3
Dendrobium candidum cellulose method for preparing morchella mycelium is made by following step:
(1) by each nutriment of prescription weighing
The dry dregs of a decoction after dendrobium candidum extracts (offcuts after extracting, generally abandon be rubbish) 90%, corn 5%, add 0.5% sodium selenite, 0.5% germanium dioxide, potassium dihydrogen phosphate 0.2%, water content 60%, pH value nature.
(2) get corn (without going mouldy, without bad grain), corn flour be broken into grain of rice size eluriate totally to be placed in the clear water and soak, filtering element (cartridge) water then, put into the steamer Steam by water bath to the white heart, pull out after draining away the water.
(3) auxiliary material potassium dihydrogen phosphate, sodium selenite and germanium dioxide are made its fully dissolving in 45 ℃ of warm water, dilution certain multiple.Mix thoroughly with iblet and cellulose.
(4) vexed 1-2 hour (day-light saving time should not be long) bottles after material fully suctions water, feeds to shoulder, tightens lower pine and flattens charge level, cleans bottleneck, sealing.
(5) dress pot sterilization autoclaving is kept and was taken the dish out of the pot in 2 hours in 126 ℃, pressure position 0.14Mpa, is cooled to below 30 ℃ and waits to inoculate.
(6) with postvaccinal bacterial classification, and cultivate under the black chamber.
The optimization method of dendrobium candidum cellulose Morciiella Esculeuta Mycelia condition of culture is as follows:
Design L
9(3
4) orthogonal test table, 3 factors are respectively: cultivation temperature (factor A), incubation time (factor B), humidity (factor C), and their addition level is as follows respectively:
From experimental result as can be known, A
2B
3C
2, the mycelium growing way is best, and hickory chick bag exo polysaccharides content is high.Therefore the condition of culture of dendrobium candidum cellulose Morchella esculenta (L.) Pers mycelium the best is: temperature is 24 ℃, and incubation time is 20 days, when medium humidity is 55%.
Claims (5)
1. method for preparing morchella mycelium take dendrobium fiber as matrix, it is characterized in that, the suitableeest mass fraction of each recipe ingredient of stem of noble dendrobium Morchella esculenta (L.) Pers mycelium solid culture medium is: dendrobium candidum is through pulverizing cellulose dregs 70%-90%, corn 5%-20%, adding 0.5%-1% sodium selenite, 0.5%-1% germanium dioxide, potassium dihydrogen phosphate 0.01%-0.2%, water content 50%-60%, the pH value nature after extracting medicinal extract;
Described preparation method may further comprise the steps:
(1) get corn, corn flour is broken into grain of rice size eluriates totally to be placed in the clear water and soak, then filtering element (cartridge) water is put into the steamer Steam by water bath to the white heart of mistake;
(2) get dendrobium fiber and the middle abundant mixing of material of step (1) that is ground into;
(3) get potassium dihydrogen phosphate, sodium selenite and germanium dioxide, the pure water that adds an amount of ratio dissolves under warm water, regulates the water content of medium, pours the material that is mixed in the step (2) into, stirs;
(4) medium in the step (3) carries out sterilization after the bottling, naturally takes out inoculation hickory chick bacterial classification after the cooling;
(5) optimization of condition of culture: under the prerequisite that medium is fixed, the condition of culture of Morciiella Esculeuta Mycelia is: temperature is 24 ℃, humidity 55%, and incubation time is 20 days, in order to obtain better suitable dendrobium candidum cellulose Morchella esculenta (L.) Pers mycelium growth conditions, design L
9(3
4) orthogonal test table, each factor is established 3 addition levels, finally optimizes the growth conditions of best suitable dendrobium fiber element Morciiella Esculeuta Mycelia;
(6) cultivate under the condition of culture of optimization, condition of culture is: temperature is 24 ℃, humidity 55%, incubation time is 20 days, cover with behind the mycelia dendrobium candidum cellulose Morchella esculenta (L.) Pers mycelium.
2. such as claim 1 method for preparing morchella mycelium take dendrobium fiber as matrix, it is characterized in that, dendrobium candidum cellulose in the described step (2) is further pulverized again, and its fluffy being convenient to is absorbed water.
3. such as claim 1 method for preparing morchella mycelium take dendrobium fiber as matrix, it is characterized in that, the 45 ℃ of dissolvings of warm water of described step (3) potassium dihydrogen phosphate, sodium selenite and germanium dioxide, water dilutes is convenient to stir controlled humidity.
4. such as claim 1 method for preparing morchella mycelium take dendrobium fiber as matrix, it is characterized in that, the method of described step (4) sterilization adopts high pressure steam sterilization, its condition is: under 0.14MPa, keep 2h under 126 ℃, naturally cooling refers to be cooled to below 30 ℃, inoculates.
5. such as claim 1 method for preparing morchella mycelium take dendrobium fiber as matrix, it is characterized in that, the condition that described step (6) is cultivated should be cultivated under dark condition, and the gained mycelium is directly used in health products and raw-food material, need not other processing.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103238464A (en) * | 2013-05-17 | 2013-08-14 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
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| CN103404370B (en) * | 2013-07-31 | 2016-01-20 | 西南科技大学 | The method of edible fungus species is cultivated with pelelith |
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| CN104541968B (en) * | 2014-12-30 | 2017-01-18 | 西林县大银子铁皮石斛种销农民专业合作社 | Dendrobium officinale and black fungus interplanting method |
| CN105900696B (en) * | 2016-06-28 | 2019-03-01 | 广西壮仁堂生物科技有限公司 | Phellinus igniarius mycelium is prepared by culture medium of dendrobium candidum |
| CN105900695B (en) * | 2016-06-28 | 2019-03-01 | 广西壮仁堂生物科技有限公司 | Cordyceps mycelia is prepared by culture medium of dendrobium candidum |
| US11920126B2 (en) | 2018-03-28 | 2024-03-05 | Ecovative Design Llc | Bio-manufacturing process |
| CN113373058A (en) * | 2021-06-08 | 2021-09-10 | 安徽省百麓现代农业科技有限公司 | Method for liquid fermentation of cordyceps militaris strains |
| CN115362889A (en) * | 2022-07-08 | 2022-11-22 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Method for interplanting strawberry and morchella |
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Cited By (2)
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| CN103238464A (en) * | 2013-05-17 | 2013-08-14 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
| CN103238464B (en) * | 2013-05-17 | 2014-10-08 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
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