CN103283608A - Factory cultivation strains of needle mushrooms, and cultivation method thereof - Google Patents

Factory cultivation strains of needle mushrooms, and cultivation method thereof Download PDF

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CN103283608A
CN103283608A CN2013102604872A CN201310260487A CN103283608A CN 103283608 A CN103283608 A CN 103283608A CN 2013102604872 A CN2013102604872 A CN 2013102604872A CN 201310260487 A CN201310260487 A CN 201310260487A CN 103283608 A CN103283608 A CN 103283608A
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bacterial classification
strains
asparagus
cultivation
factory
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CN103283608B (en
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郝辰
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Shenyang Hengsheng Biotechnology Development Co Ltd
SHENYANG HENGSHENG AGRICULTURAL DEVELOPMENT Co Ltd
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Shenyang Hengsheng Biotechnology Development Co Ltd
SHENYANG HENGSHENG AGRICULTURAL DEVELOPMENT Co Ltd
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Abstract

The invention discloses a cultivation method of factory cultivation strains of needle mushrooms applied to the technical field of a technology of factory production of edible mushrooms. The cultivation method of the factory cultivation strains of the needle mushrooms comprises the following steps of: carrying out cultivating, spawn running, mushroom management and recovery by taking needle mushrooms HS-JG01 as mother strains; transferring the mother strains to a plate culture medium to form F1-generation strains and a solid culture medium when plate culture is adopted to cultivate; transferring the F1-generation strains cultivated by the mother strains to a liquid culture medium to form F2-generation strains and a liquid culture medium when liquid culture is adopted; cultivating breeder seeds and cultivated species from the mother strains when bottle cultivation factory training is adopted, and then inoculating the cultivated species to bottled culture materials. The needle mushrooms HS-JG01 disclosed by the invention contain a plurality of amino acids, and are named 'intelligence-promoting mushrooms' because of the function of promoting mental development of children; the needle mushrooms contain flammulin, which has an antitumous effect and can be used for preventing hypertension, and treating liver and intestines and stomach ulcers; the needle mushrooms are delicious foods, and good health-care foods.

Description

A kind of Asparagus factory culture bacterial classification and cultivation method thereof
Technical field
The present invention relates to a kind of Asparagus factory culture bacterial classification and cultivation method thereof in the technology field that edible fungus industrial produces, particularly the preparation of the batch production bottle cultivation strain of bottle cultivated edible fungi and cultivation method thereof.
Background technology
Edible mushroom mainly is divided into two classes, and saprophytic bacteria and mycorhiza bacterium also can't carry out artificial cultivation till the mycorhiza Zoopagales is preceding; And saprophytic bacteria is to carry out factory culture.Edible Fungus's Industrialized Production in China is started late, and for edible fungus variety, the golden mushroom plantation technology is ripe relatively.The batch production production of Asparagus mainly contains that bottle is planted and bag is planted two kinds of production models, and the batch production manufacturing enterprise that mechanization degree is high adopts bottle to plant system.Bottle is planted the standardized production technological process: bacterium-urge flower bud-suppress-give birth to-gather-dig bottle-waste recovery utilization is cultivated-disturbed to batching-spice-bottling-sterilization-cooling-inoculation-Fa bacterium.The preparation of Asparagus factory culture bacterial classification is the key link during edible fungus industrial is produced, in production of hybrid seeds process, medium sterilization thoroughly and the inoculation operation etc. reason such as lack of standardization all pollute easily, grasping the bacterial classification manufacturing technology is the key that control is polluted.My company is engaged in the Asparagus bacterial classification and produced and cultivate existing 7 years, but its fraction defective pollution rate control is in 1%.Asparagus bacterial classification production routine is divided into female kind, original seed, 3 levels of cultivated species usually, and its manufacturing process comprises medium preparation, inoculation, cultural hypha etc.Plant in the Asparagus batch production production at bottle, adopt unique production of hybrid seeds and the production technology of factory culture, effectively raise bottle and plant yield rate and the product quality that the Asparagus batch production is produced.Therefore, develop a kind of Asparagus factory culture bacterial classification and cultivation method thereof is the problem that needs to be resolved hurrily always.
Summary of the invention
The object of the present invention is to provide a kind of Asparagus factory culture bacterial classification and cultivation method thereof, plant the bacterial classification that Asparagus is produced with the batch production bottle, behind artificial culture, be used for the batch production production of Asparagus.
Asparagus of the present invention (Flammulina velutipes) HS-JG01 has been deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on May 2nd, 2013, be called for short CGMCC, and the address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.Deposit number is: CGMCC No.7705.
Asparagus of the present invention (Flammulina velutipes) HS-JG01 confirms that through the experimental data analysis-by-synthesis such as cultural characteristic, microscopic features and rRNA gene order of institute of microbiology of the Chinese Academy of Sciences qualification result of HS-JG01 is Flammulina velutipes Asparagus.
A kind of Asparagus factory culture bacterial classification, described Asparagus factory culture bacterial classification is Asparagus HS-JG01, deposit number is: CGMCC No.7705.
The cultivation method of described Asparagus factory culture bacterial classification serves as that female the kind cultivated, sent out bacterium, management of producing mushroom and gather with Asparagus HS-JG01; When adopting plate to cultivate: mother's kind is transferred to the plate medium forms F 1For bacterial classification, solid culture medium: PDA; When adopting liquid culture, mother is planted the F that cultivates 1Be transferred to for bacterial classification and form F in the liquid nutrient medium 2For bacterial classification, liquid nutrient medium: PDB; With F 2Be transferred to for bacterial classification and form F in the fermentation tank 3For bacterial classification, fermentation tank culture material composition: bean cake powder 4%, white granulated sugar 1%, defoamer 0.02%, water 94.98%, natural pH value is carried out autoclave sterilization, is cooled to 16-19 ℃ then; When adopting bottle to plant the batch production cultivation, mother's kind is cultivated original seed and cultivated species, again cultivated species is inoculated on the bottled composts or fertilisers of cultivating.
Described bottled composts or fertilisers of cultivating: corncob 17-19%, rice bran 13-15%, corn flour 1%, beet 2-3%, calcium carbonate 0.4%, dregs of beans 0.4%, water 62-65%, pH7.0.
Asparagus of the present invention (Flammulina velutipes) HS-JG01 has following characteristic:
Asparagus formal name used at school hair handle money bacterium is commonly called as structure bacterium, plain mushroom, dried mushroom, intelligence mushroom etc. and belongs to Agaricales.Have that stem is tender and crisp, cap stick-slip, delicious flavour.
Beneficial effect of the present invention: Asparagus (Flammulina velutipes) HS-JG01 contains several amino acids, and especially lysine, arginine content are high especially, has the function that promotes children ' s intelligence development so be called " increasing the intelligence mushroom " again; Studies show that, contained Flammulin has remarkable antitumaous effect in the Asparagus, often eat and to prevent hypertension and treatment liver and intestine gastric ulcer, so Asparagus is a kind of ticbit, it is again good health food, Asparagus at home and abroad is loved by the people in market at present, and supply falls short of demand for product.
Product colour purity of the present invention is product in vain, and disease resistance is strong, the output height, and a type is good, and base portion fine hair is few.
Description of drawings
The present invention is described in detail below in conjunction with drawings and Examples.
Fig. 1 is Asparagus batch production production production of hybrid seeds process chart of the present invention.
Fig. 2 is Asparagus of the present invention factory planting technique flow chart.
Fig. 3 is that the present invention is at microscopically mycelia situation map.
Fig. 4 is F of the present invention 2Cultivate figure for the bacterial classification triangular flask.
Fig. 5 is F of the present invention 3Cultivate figure for the strain fermentation jar.
Fig. 6 is that bottle of the present invention is planted a bacterium situation map.
Fig. 7 is that bottle of the present invention is planted the fruiting situation map.
Embodiment
A kind of Asparagus factory culture bacterial classification, described Asparagus factory culture bacterial classification are Asparagus (Flammulina velutipes) HS-JG01, and deposit number is: CGMCC No.7705.
Asparagus of the present invention (Flammulina velutipes) HS-JG01 plants the bacterial classification that Asparagus is produced with the batch production bottle, is used for the batch production production of Asparagus behind artificial culture.
Asparagus batch production production production of hybrid seeds technological process (Fig. 1): the strain preparation technological process mainly comprises:
(1) culture dish F 1For strain preparation and cultivation: preparation PDA (potato dextrose agar) solid culture medium, sterilize, divide to be filled in the culture dish.Mother's kind (fruit body) is transferred in the plate medium 16-18 ℃ of temperature, dark culturing, 16-18 days.
(2) triangular flask F 2Prepare and cultivation for liquid spawn: preparation PDB (liquid potato glucose medium) liquid nutrient medium, divide to be filled in the triangular flask, sterilize.With the better female culture dish F that cultivates that plants of growth conditions 1For bacterial classification inoculation in triangular flask, 16-18 ℃ of temperature, 120 rev/mins, dark culturing, 7-8 days.
(3) fermentation tank is got the raw materials ready, is sterilized and cools off: fermentation tank culture material composition: bean cake powder 4g, and white granulated sugar 1g, defoamer 0.02g, water 94.98g, natural pH value is carried out autoclave sterilization, is cooled to 16-19 ℃ then.
(4) fermentation tank F 3For strain preparation and cultivation: with F 2Be transferred to for bacterial classification and form F in the fermentation tank 3For bacterial classification, 16-18 ℃ of temperature, throughput is 1.2-1.7m 3/ h cultivated 7-8 days.
When adopting bottle to plant the batch production cultivation, mother's kind is cultivated original seed and cultivated species, again cultivated species is inoculated on the bottled composts or fertilisers of cultivating.Asparagus factory planting technique flow process (Fig. 2): the planting technique flow process mainly comprises:
(1) composts or fertilisers of cultivating preparation: corncob 18.2g, rice bran 14g, corn flour 1g, beet 3g, calcium carbonate 0.4g, dregs of beans 0.4g, water 63g, pH7.0.According to proportioning corncob is added water and stirred 15 minutes, it is moisture 45% to add water to composts or fertilisers of cultivating again, prewets and stirs after 60 minutes, adds other batching and stirs and add water, to water content 62-65%, stirs 15 minutes again.With the composts or fertilisers of cultivating bottling after stirring, gland and entrucking.
(2) composts or fertilisers of cultivating sterilization and cooling: with the culture bottle entrucking, push Sterilization Kettle and carry out autoclave sterilization, push fore-cooling room and cooling chamber afterwards, progressively cool off, be cooled to 14-17 ℃.
(3) strain preparation and inoculation: strain preparation reference fluids strain preparation technological process (Fig. 1).The fermentation tank bacterial classification is seeded in the culture bottle by liquid inoculation device, and inoculum concentration is 20-25g.
(4) cultural hypha: postvaccinal blake bottle is transferred to culturing room, carries out dark culturing, between 15-18 ℃ of the room temperatures, humidity 60-65% suitably feeds new wind, CO 2Concentration is below 4000ppm, and cultivating fate is 22-25 days.
(5) mycelium stimulation is washed bacterium: after mycelia is covered with in the blake bottle, carry out mechanical mycelium stimulation and wash bacterium, remove aging bacterium layer.
(6) urge the flower bud phase: temperature is controlled within 14-17 ℃ of scopes, and humidity 90-95% gives certain scattered light, and illumination is advisable with 600-800LX, 8-10 days.
(7) inhibition period: temperature progressively is down to 3-5 ℃, humidity 90%, 7-8 days.
(8) educate the mushroom phase: temperature is controlled at 8-10 ℃, humidity 90-95%, 9-10 days, and ventilation reduces.
(9) processing of gathering: long to suitable size when young mushroom, gather and process.
F 1For bacterial classification: mother's kind (fruit body) is transferred to the bacterial classification for preparing in the plate medium;
F 2For liquid spawn: with the better female culture dish F that cultivates that plants of growth conditions 1The bacterial classification for preparing in the triangular flask for bacterial classification inoculation;
F 3For bacterial classification: with F 2Be transferred to the cultivation bacterial classification of making in the fermentation tank for bacterial classification.

Claims (3)

1. Asparagus factory culture bacterial classification, it is characterized in that: described Asparagus factory culture bacterial classification is Asparagus HS-JG01, and deposit number is: CGMCC No.7705.
2. the cultivation method of an Asparagus factory culture bacterial classification as claimed in claim 1 is characterized in that: the cultivation method of described Asparagus factory culture bacterial classification serves as that female the kind cultivated, sent out bacterium, management of producing mushroom and gather with Asparagus HS-JG01; When adopting plate to cultivate: mother's kind is transferred to the plate medium forms F 1For bacterial classification, solid culture medium: PDA; When adopting liquid culture, mother is planted the F that cultivates 1Be transferred to for bacterial classification and form F in the liquid nutrient medium 2For bacterial classification, liquid nutrient medium: PDB; With F 2Be transferred to for bacterial classification and form F in the fermentation tank 3For bacterial classification, fermentation tank culture material composition: bean cake powder 4%, white granulated sugar 1%, defoamer 0.02%, water 94.98%, natural pH value is carried out autoclave sterilization, is cooled to 16-19 ℃ then; When adopting bottle to plant the batch production cultivation, mother's kind is cultivated original seed and cultivated species, again cultivated species is inoculated on the bottled composts or fertilisers of cultivating.
3. the cultivation method of an Asparagus factory culture bacterial classification as claimed in claim 1 or 2, it is characterized in that: described bottled composts or fertilisers of cultivating: corncob 17-19%, rice bran 13-15%, corn flour 1%, beet 2-3%, calcium carbonate 0.4%, dregs of beans 0.4%, water 62-65%, pH7.0.
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103980048A (en) * 2014-05-22 2014-08-13 徐州绿维现代农业科技有限公司 Preparation raw material and cultivation method of flammulina velutipes
CN105248285A (en) * 2015-09-24 2016-01-20 中国科学院昆明植物研究所 Flammulina velutipes variety, and cultivation method thereof
CN107691116A (en) * 2017-10-26 2018-02-16 江苏华绿生物科技股份有限公司 A kind of Mother culture method and its application of liquid spawn
CN107980474A (en) * 2017-12-05 2018-05-04 罗成喜 A kind of cultural method of needle mushroom
CN109452088A (en) * 2018-09-28 2019-03-12 上海雪榕生物科技股份有限公司 Needle mushroom X18 and its cultural method
CN110073896A (en) * 2019-06-03 2019-08-02 沈阳恒生生物科技发展有限公司 Oyster cap fungus factory culture strain and cultural method are produced using needle mushroom mushroom bran
CN112280690A (en) * 2020-10-20 2021-01-29 江苏华绿生物科技股份有限公司 Method for rejuvenating and screening needle mushroom strains

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CN103004473A (en) * 2012-12-29 2013-04-03 沈阳恒生农业发展有限公司 Cooling technique for increasing inoculating yield in industrial production of bottled golden needle mushrooms

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103980048A (en) * 2014-05-22 2014-08-13 徐州绿维现代农业科技有限公司 Preparation raw material and cultivation method of flammulina velutipes
CN105248285A (en) * 2015-09-24 2016-01-20 中国科学院昆明植物研究所 Flammulina velutipes variety, and cultivation method thereof
CN107691116A (en) * 2017-10-26 2018-02-16 江苏华绿生物科技股份有限公司 A kind of Mother culture method and its application of liquid spawn
CN107980474A (en) * 2017-12-05 2018-05-04 罗成喜 A kind of cultural method of needle mushroom
CN109452088A (en) * 2018-09-28 2019-03-12 上海雪榕生物科技股份有限公司 Needle mushroom X18 and its cultural method
CN109452088B (en) * 2018-09-28 2021-05-25 上海雪榕生物科技股份有限公司 Flammulina velutipes X18 and cultivation method thereof
CN110073896A (en) * 2019-06-03 2019-08-02 沈阳恒生生物科技发展有限公司 Oyster cap fungus factory culture strain and cultural method are produced using needle mushroom mushroom bran
CN112280690A (en) * 2020-10-20 2021-01-29 江苏华绿生物科技股份有限公司 Method for rejuvenating and screening needle mushroom strains

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