CN103922851A - Black fungus liquid medium and black fungus cultural method - Google Patents
Black fungus liquid medium and black fungus cultural method Download PDFInfo
- Publication number
- CN103922851A CN103922851A CN201410174040.8A CN201410174040A CN103922851A CN 103922851 A CN103922851 A CN 103922851A CN 201410174040 A CN201410174040 A CN 201410174040A CN 103922851 A CN103922851 A CN 103922851A
- Authority
- CN
- China
- Prior art keywords
- black fungus
- medium
- inoculation
- cultural method
- dregs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a black fungus liquid medium and a black fungus cultural method. The black fungus liquid medium is formed by dissolving the following components in each liter of water: 250-400ml of soybean meal enzymatic hydrolysate, 15-45g of glucose, 1-3g of sodium selenite, 1.5-3.5g of potassium iodate, 1-4g of zinc sulphate, 1-2g of magnesium sulphate and 20-30mg of vitamin B1. The black fungus liquid medium has the characteristics of convenience in preparation, low cost and abundant trace elements; compared with the traditional black fungus cultural method, the black fungus cultural method has the advantage that black fungus culture time is effectively shortened, thereby being applicable to industrialized promotion.
Description
Technical field
The present invention relates to Edible mushroom processing technical field, particularly relate to a kind of black fungus liquid nutrient medium and cultural method thereof.
Background technology
Black fungus (Auricularia auricula) belongs to Mycophyta, Basidiomycetes, Auriculariale, Auriculariaceae, Auricularia, is a kind of famous dietotherapeutic macro fungi, mycelium and sporophore, consists of.Auricularia polysaccharide is the main active ingredient of auricularia auriculajudae, has the multiple biological activitys such as antitumor, antithrombotic, anti-ageing, hypoglycemic, reducing blood-fat and adjusting immunologic function.In addition, the iron level in black fungus is that in various meat and vegetable foods, content is the abundantest, and edible black fungus can be in time for human body supplements enough ironys.Mycelium is the vegetative organ of black fungus, is equivalent to root, stem, the leaf of high green plants, is the main body of black fungus.The sporophore of black fungus, claims again monospermous fruit, is that we cook edible part.
The cultivating method of black fungus has formed fixing pattern at present, being about to wood chip, wheat bran, cotton seed skin, crops stalk crushing thing, mineral substance etc. packs in special-purpose high-temperature resistance plastice bag, through High Temperature Sterilization, inoculation, cultivation, scarfing, go out ear, while going out ear, maybe will go out the hanging of ear bag in canopy chamber, maybe will go out the setting of ear bag puts on the ground, but cultivating on a large scale ubiquity yields poorly, poor quality, pollution rate is high, the problems such as output is unstable, have become the bottleneck that bag carries auricularia auriculajudae development, are badly in need of being resolved and improving.
In Edible mushroom processing technical field, there are some utilizations under liquid condition, to cultivate the relevant report of thalline, it is fast that they have thalli growth, and condition is the advantage such as control easily.
Summary of the invention
The object of the invention is for the technological deficiency existing in prior art, and a kind of liquid nutrient medium and cultural method thereof of applicable black fungus Fast Growth are provided.
For realizing the technical scheme that object of the present invention adopts, be: a kind of black fungus liquid nutrient medium, it is characterized in that thering is following proportioning: in every 1L water, dissolve dregs of beans enzymolysis solution 250-400ml, glucose 15-45g, Sodium Selenite 1-3g, Potassium Iodate 1.5-3.5g, zinc sulfate 1-4g, magnesium sulfate 1-2g and vitamins B
120-30mg; Described dregs of beans enzymolysis solution be by dregs of beans with etc. the water of quality mix, reconciling pH value is 8-10, adds subsequently the Sumizyme MP of 4000-6000U/g, be hydrolyzed 2-3h at 55-70 ℃ after, boil hydrolyzed solution, after boiling 20-30min, use layer 2-4 filtered through gauze, obtain dregs of beans enzymolysis solution.
Preferably, the proportioning of black fungus liquid nutrient medium is: in every 1L water, dissolve dregs of beans enzymolysis solution 350mL, glucose 40g, Sodium Selenite 2.5g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g and vitamins B
130mg.
A cultural method for black fungus liquid nutrient medium, is characterized in that comprising the following steps:
(1) packing: by the liquid culture medium 50-100mL of above-mentioned preparation, divide in the triangular flask that is filled to 250mL and seal;
(2) sterilizing: by the triangular flask sterilizing of step (1) sealing, obtain sterilising medium;
(3) inoculation: plant the one-level auricularia auriculajudae of purifying after activation is female, be linked in the cooled sterilising medium that step (2) obtains, obtain inoculation medium;
(4) cultivate: the inoculation medium that step (3) is obtained shakes cultivation in shaking table, after cultivating, obtain second class inoculum.
Described in described step (2), the temperature of sterilizing is 115-120 ℃, and sterilization time is 10-15min.
The inoculum size of sterilising medium described in described step (3) accesses 1-2 piece 0.5-1.0cm in each triangular flask
2bacterium piece.
Described in described step (4), the culture temperature of inoculation medium is 23-28 ℃, incubation time 4 days, shaking table concussion speed 150-200rpm.
A cultural method for black fungus liquid nutrient medium, is characterized in that specifically comprising the following steps:
(1) packing: the liquid culture medium preparing is packed in 250mL triangular flask by the loading amount of every bottle of 50-100mL, and eight layers of gauze, bind kraft paper sealing beyond the Great Wall;
(2) sterilizing, by step (1) gained sealing triangular flask, at 115-120 ℃, sterilizing 10-15min, obtains sterilising medium;
(3) inoculation: by the female kind of the one-level auricularia auriculajudae of purifying after activation, be linked in the sterilising medium of step (2) gained, inoculum size is every bottle of 1-2 piece 0.5-1.0cm
2bacterium piece, obtain inoculation medium;
(4) cultivate: the shaking table concussion that step (3) gained inoculation medium is placed in to 150-200rpm is cultivated, and cultivates 4 days at 23-28 ℃, obtains second class inoculum.
Principle of the present invention is: the dregs of beans of usining in substratum is made nitrogenous source, glucose is made carbon source, Sodium Selenite as selenium source, and adds a small amount of magnesium sulfate, zinc sulfate and Potassium Iodate; Magnesium elements is the activator of many enzymes, can promote the metabolism of carbohydrate, synthetic, the phosphatic conversion of nucleic acid in substratum; Zinc sulfate can be used for preventing disease, and it is also a kind of fertilizer of supplementary black fungus zinc microelement; Potassium Iodate joins in substratum and not only can contribute to black fungus to produce as a kind of foodstuff additive that are allowed to, and can promote iodine content useful to human body in black fungus.
The present invention relates to a kind of liquid nutrient medium of applicable black fungus Fast Growth, its beneficial effect is: have the advantages that preparation is convenient, cost is low, trace element is abundant, than the cultural method of traditional black fungus, effectively shortened the incubation time of black fungus, be applicable to Industry Promotion.Black fungus can reach higher biomass and enough bacterium nodule numbers for 4 days in substratum provided by the invention, can be the later stage to carry out artificial culture sufficient second class inoculum is provided, and is conducive to the propelling of artificial culture research.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1
A liquid nutrient medium for applicable black fungus Fast Growth, the consisting of of its composition of raw materials: dregs of beans enzymolysis solution 250mL, glucose 15g, Sodium Selenite 1g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
120mg, water 1000mL.
By above-mentioned formula, after being mixed with the water of equivalent, boils dregs of beans, and after boiling 30min, the dregs of beans enzymolysis solution 250mL obtaining after filtration, with glucose 15g, Sodium Selenite 1g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
120mg and water 1000mL mix.
The method that application aforesaid liquid substratum is cultivated black fungus, step is as follows:
1) packing: the liquid culture medium preparing is packed in 250mL triangular flask by the loading amount of every bottle of 80mL, and eight layers of gauze, bind kraft paper sealing beyond the Great Wall;
2) sterilizing, by step 1) gained sealing triangular flask is at 120 ℃, and sterilizing 10min, obtains sterilising medium;
3) inoculation: by the female kind of the one-level auricularia auriculajudae of purifying after activation, be linked into step 2), in the sterilising medium of gained, inoculum size is every bottle of 2 0.5-1.0cm
2bacterium piece, obtain inoculation medium;
4) cultivation: by step 3) gained inoculation medium is placed in the shaking table concussion cultivation of 170rpm, cultivates 4 days at 25 ℃, obtains second class inoculum.
The black fungus that the present embodiment 1 is produced, mycelium pellet quantity is many, small and dense reality, mycelia dry weight reaches 6.3g/L, is rich in the various trace elements such as iron, zinc, selenium, iodine, magnesium simultaneously.
Embodiment 2
A liquid nutrient medium for applicable black fungus Fast Growth, the consisting of of its composition of raw materials: dregs of beans enzymolysis solution 400mL, glucose 45g, Sodium Selenite 3g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
130mg, water 1000mL.
By above-mentioned formula, after being mixed with the water of equivalent, boils dregs of beans, and after boiling 30min, the dregs of beans enzymolysis solution 400mL obtaining after filtration, with glucose 45g, Sodium Selenite 3g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
130mg and water 1000mL mix.
The method that application aforesaid liquid substratum is cultivated black fungus, step is as follows:
1) packing: the liquid culture medium preparing is packed in 250mL triangular flask by the loading amount of every bottle of 80mL, and eight layers of gauze, bind kraft paper sealing beyond the Great Wall;
2) sterilizing, by step 1) gained sealing triangular flask is at 120 ℃, and sterilizing 10min, obtains sterilising medium;
3) inoculation: by the female kind of the one-level auricularia auriculajudae of purifying after activation, be linked into step 2), in the sterilising medium of gained, inoculum size is every bottle of 2 0.5-1.0cm
2bacterium piece, obtain inoculation medium;
4) cultivation: by step 3) gained inoculation medium is placed in the shaking table concussion cultivation of 170rpm, cultivates 4 days at 25 ℃, obtains second class inoculum.
The black fungus that the present embodiment 2 is produced, mycelium pellet quantity is many, small and dense reality, mycelia dry weight reaches 13.7g/L, is rich in the various trace elements such as iron, zinc, selenium, iodine, magnesium simultaneously.
Embodiment 3
A liquid nutrient medium for applicable black fungus Fast Growth, the consisting of of its composition of raw materials: dregs of beans enzymolysis solution 350mL, glucose 30g, Sodium Selenite 2.5g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
125mg, water 1000mL.
By above-mentioned formula, after being mixed with the water of equivalent, boils dregs of beans, and after boiling 30min, the dregs of beans enzymolysis solution 350mL obtaining after filtration, with glucose 30g, Sodium Selenite 2.5g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
125mg and water 1000mL mix.
The method that application aforesaid liquid substratum is cultivated black fungus, step is as follows:
1) packing: the liquid culture medium preparing is packed in 250mL triangular flask by the loading amount of every bottle of 80mL, and eight layers of gauze, bind kraft paper sealing beyond the Great Wall;
2) sterilizing, by step 1) gained sealing triangular flask is at 120 ℃, and sterilizing 10min, obtains sterilising medium;
3) inoculation: by the female kind of the one-level auricularia auriculajudae of purifying after activation, be linked into step 2), in the sterilising medium of gained, inoculum size is every bottle of 2 0.5-1.0cm
2bacterium piece, obtain inoculation medium;
4) cultivation: by step 3) gained inoculation medium is placed in the shaking table concussion cultivation of 170rpm, cultivates 4 days at 25 ℃, obtains second class inoculum.
The black fungus that the present embodiment 3 is produced, mycelium pellet quantity is many, small and dense reality, mycelia dry weight reaches 10.8g/L, is rich in the various trace elements such as iron, zinc, selenium, iodine, magnesium simultaneously.
Embodiment 4
A liquid nutrient medium for applicable black fungus Fast Growth, the consisting of of its composition of raw materials: dregs of beans enzymolysis solution 350mL, glucose 30g, Sodium Selenite 2.5g, Potassium Iodate 3.5g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
125mg, water 1000mL.
By above-mentioned formula, after being mixed with the water of equivalent, boils dregs of beans, and after boiling 30min, the dregs of beans enzymolysis solution 350mL obtaining after filtration, with glucose 30g, Sodium Selenite 2.5g, Potassium Iodate 3.5g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
125mg and water 1000mL mix.
The method that application aforesaid liquid substratum is cultivated black fungus, step is as follows:
1) packing: the liquid culture medium preparing is packed in 250mL triangular flask by the loading amount of every bottle of 100mL, and eight layers of gauze, bind kraft paper sealing beyond the Great Wall;
2) sterilizing, by step 1) gained sealing triangular flask is at 120 ℃, and sterilizing 10min, obtains sterilising medium;
3) inoculation: by the female kind of the one-level auricularia auriculajudae of purifying after activation, be linked into step 2), in the sterilising medium of gained, inoculum size is every bottle of 2 0.5-1.0cm
2bacterium piece, obtain inoculation medium;
4) cultivation: by step 3) gained inoculation medium is placed in the shaking table concussion cultivation of 200rpm, cultivates 4 days at 25 ℃, obtains second class inoculum.
The black fungus that the present embodiment 4 production methods are produced, mycelium pellet quantity is many, small and dense reality, mycelia dry weight reaches 12.8g/L, is rich in the various trace elements such as iron, zinc, selenium, iodine, magnesium simultaneously.
Embodiment 5
A liquid nutrient medium for applicable black fungus Fast Growth, the consisting of of its composition of raw materials: dregs of beans enzymolysis solution 350mL, glucose 40g, Sodium Selenite 2.5g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
130mg, water 1000mL.
By above-mentioned formula, after being mixed with the water of equivalent, boils dregs of beans, and after boiling 30min, the dregs of beans enzymolysis solution 350mL obtaining after filtration, with glucose 40g, Sodium Selenite 2.5g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
130mg and water 1000mL mix.
The method that application aforesaid liquid substratum is cultivated black fungus, step is as follows:
1) packing: the liquid culture medium preparing is packed in 250mL triangular flask by the loading amount of every bottle of 100mL, and eight layers of gauze, bind kraft paper sealing beyond the Great Wall;
2) sterilizing, by step 1) gained sealing triangular flask is at 120 ℃, and sterilizing 10min, obtains sterilising medium;
3) inoculation: by the female kind of the one-level auricularia auriculajudae of purifying after activation, be linked into step 2), in the sterilising medium of gained, inoculum size is every bottle of 2 0.5-1.0cm
2bacterium piece, obtain inoculation medium;
4) cultivation: by step 3) gained inoculation medium is placed in the shaking table concussion cultivation of 200rpm, cultivates 4 days at 25 ℃, obtains second class inoculum.
The black fungus that the present embodiment 5 is produced, mycelium pellet quantity is many, small and dense reality, mycelia dry weight reaches 14.2g/L, is rich in the various trace elements such as iron, zinc, selenium, iodine, magnesium simultaneously.
Embodiment 6
A liquid nutrient medium for applicable black fungus Fast Growth, the consisting of of its composition of raw materials: dregs of beans enzymolysis solution 250mL, glucose 30g, Sodium Selenite 1g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
120mg, water 1000mL.
By above-mentioned formula, after being mixed with the water of equivalent, boils dregs of beans, and after boiling 30min, the dregs of beans enzymolysis solution 250mL obtaining after filtration, with glucose 30g, Sodium Selenite 1g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g, vitamins B
120mg and water 1000mL mix.
The method that application aforesaid liquid substratum is cultivated black fungus, step is as follows:
1) packing: the liquid culture medium preparing is packed in 250mL triangular flask by the loading amount of every bottle of 100mL, and eight layers of gauze, bind kraft paper sealing beyond the Great Wall;
2) sterilizing, by step 1) gained sealing triangular flask is at 120 ℃, and sterilizing 10min, obtains sterilising medium;
3) inoculation: by the female kind of the one-level auricularia auriculajudae of purifying after activation, be linked into step 2), in the sterilising medium of gained, inoculum size is every bottle of 1 0.5-1.0cm
2bacterium piece, obtain inoculation medium;
4) cultivation: by step 3) gained inoculation medium is placed in the shaking table concussion cultivation of 200rpm, cultivates 4 days at 25 ℃, obtains second class inoculum.
The black fungus that the present embodiment 6 is produced, mycelium pellet quantity is many, small and dense reality, mycelia dry weight reaches 8.3g/L, is rich in the various trace elements such as iron, zinc, selenium, iodine, magnesium simultaneously.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. a black fungus liquid nutrient medium, is characterized in that, has following proportioning: in every 1L water, dissolve dregs of beans enzymolysis solution 250-400ml, glucose 15-45g, Sodium Selenite 1-3g, Potassium Iodate 1.5-3.5g, zinc sulfate 1-4g, magnesium sulfate 1-2g and vitamins B
120-30mg; Described dregs of beans enzymolysis solution be by dregs of beans with etc. the water of quality mix, reconciling pH value is 8-10, adds subsequently the Sumizyme MP of 4000-6000U/g, be hydrolyzed 2-3h at 55-70 ℃ after, boil hydrolyzed solution, after boiling 20-30min, use layer 2-4 filtered through gauze, obtain dregs of beans enzymolysis solution.
2. preferred a kind of black fungus liquid nutrient medium according to claim 1, it is characterized in that thering is following proportioning: in every 1L water, dissolve dregs of beans enzymolysis solution 350mL, glucose 40g, Sodium Selenite 2.5g, Potassium Iodate 2g, zinc sulfate 3g, magnesium sulfate 1.5g and vitamins B
130mg.
3. the cultural method of a kind of black fungus liquid nutrient medium according to claim 1 and 2, is characterized in that comprising the following steps:
(1) packing: by the liquid culture medium 50-100mL of above-mentioned preparation, divide in the triangular flask that is filled to 250mL and seal;
(2) sterilizing: by the triangular flask sterilizing of step (1) sealing, obtain sterilising medium;
(3) inoculation: plant the one-level auricularia auriculajudae of purifying after activation is female, be linked in the cooled sterilising medium that step (2) obtains, obtain inoculation medium;
(4) cultivate: the inoculation medium that step (3) is obtained shakes cultivation in shaking table, after cultivating, obtain second class inoculum.
4. the cultural method of a kind of black fungus liquid nutrient medium according to claim 3, the temperature that it is characterized in that sterilizing described in described step (2) is 115-120 ℃, sterilization time is 10-15min.
5. the cultural method of a kind of black fungus liquid nutrient medium according to claim 3, is characterized in that the inoculum size of sterilising medium described in described step (3) accesses 1-2 piece 0.5-1.0cm in each triangular flask
2bacterium piece.
6. the cultural method of a kind of black fungus liquid nutrient medium according to claim 3, the culture temperature that it is characterized in that inoculation medium described in described step (4) is 23-28 ℃, incubation time 4 days, shaking table concussion speed 150-200rpm.
7. the cultural method of a kind of black fungus liquid nutrient medium according to claim 1 and 2, is characterized in that comprising the following steps:
(1) packing: the liquid culture medium preparing is packed in 250mL triangular flask by the loading amount of every bottle of 50-100mL, and eight layers of gauze, bind kraft paper sealing beyond the Great Wall;
(2) sterilizing, by step (1) gained sealing triangular flask, at 115-120 ℃, sterilizing 10-15min, obtains sterilising medium;
(3) inoculation: by the female kind of the one-level auricularia auriculajudae of purifying after activation, be linked in the sterilising medium of step (2) gained, inoculum size is every bottle of 1-2 piece 0.5-1.0cm
2bacterium piece, obtain inoculation medium;
(4) cultivate: the shaking table concussion that step (3) gained inoculation medium is placed in to 150-200rpm is cultivated, and cultivates 4 days at 23-28 ℃, obtains second class inoculum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410174040.8A CN103922851A (en) | 2014-04-28 | 2014-04-28 | Black fungus liquid medium and black fungus cultural method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410174040.8A CN103922851A (en) | 2014-04-28 | 2014-04-28 | Black fungus liquid medium and black fungus cultural method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103922851A true CN103922851A (en) | 2014-07-16 |
Family
ID=51141280
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410174040.8A Pending CN103922851A (en) | 2014-04-28 | 2014-04-28 | Black fungus liquid medium and black fungus cultural method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103922851A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134757A (en) * | 2015-04-03 | 2016-11-23 | 池州市德丰菌业有限责任公司 | A kind of zinc-rich selenium mushroom cultivating method |
| CN106134756A (en) * | 2015-04-03 | 2016-11-23 | 池州市德丰菌业有限责任公司 | A kind of cultural method of selenium-rich zinc black Auricularia |
| CN106852256A (en) * | 2016-12-30 | 2017-06-16 | 江苏品品鲜生物科技有限公司 | A kind of composite cultivation method of black fungus |
| CN107236674A (en) * | 2017-06-26 | 2017-10-10 | 浦江县美泽生物科技有限公司 | Edible fungus culture medium and preparation method thereof |
| CN107353061A (en) * | 2017-07-26 | 2017-11-17 | 兰溪市奥而特农业科技有限公司 | A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing |
| CN107586723A (en) * | 2017-07-26 | 2018-01-16 | 浦江县欧立生物技术有限公司 | The cultural method of flammulina velutipes liquid strains |
| CN108703312A (en) * | 2018-05-25 | 2018-10-26 | 广东日可威富硒食品有限公司 | A kind of vegetable nutrient reconciles noodles and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR920006706B1 (en) * | 1990-05-15 | 1992-08-17 | 유용준 | Method for culturing pleurotus estreatus |
| CN101253970A (en) * | 2008-04-11 | 2008-09-03 | 贵州大学 | Black edible fungus health care oral liquid and method of preparing the same |
| CN101979531A (en) * | 2010-09-26 | 2011-02-23 | 湖北国力生物技术开发有限公司 | Liquid fermentation method for producing natto kinase in high yield |
| CN103330203A (en) * | 2013-06-28 | 2013-10-02 | 江苏农林职业技术学院 | Functional black fungus and kudzuvine root food and preparation method thereof |
| CN103333768A (en) * | 2013-06-28 | 2013-10-02 | 江苏农林职业技术学院 | Black fungus-radix puerariae fruit wine and preparation method thereof |
-
2014
- 2014-04-28 CN CN201410174040.8A patent/CN103922851A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR920006706B1 (en) * | 1990-05-15 | 1992-08-17 | 유용준 | Method for culturing pleurotus estreatus |
| CN101253970A (en) * | 2008-04-11 | 2008-09-03 | 贵州大学 | Black edible fungus health care oral liquid and method of preparing the same |
| CN101979531A (en) * | 2010-09-26 | 2011-02-23 | 湖北国力生物技术开发有限公司 | Liquid fermentation method for producing natto kinase in high yield |
| CN103330203A (en) * | 2013-06-28 | 2013-10-02 | 江苏农林职业技术学院 | Functional black fungus and kudzuvine root food and preparation method thereof |
| CN103333768A (en) * | 2013-06-28 | 2013-10-02 | 江苏农林职业技术学院 | Black fungus-radix puerariae fruit wine and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134757A (en) * | 2015-04-03 | 2016-11-23 | 池州市德丰菌业有限责任公司 | A kind of zinc-rich selenium mushroom cultivating method |
| CN106134756A (en) * | 2015-04-03 | 2016-11-23 | 池州市德丰菌业有限责任公司 | A kind of cultural method of selenium-rich zinc black Auricularia |
| CN106852256A (en) * | 2016-12-30 | 2017-06-16 | 江苏品品鲜生物科技有限公司 | A kind of composite cultivation method of black fungus |
| CN107236674A (en) * | 2017-06-26 | 2017-10-10 | 浦江县美泽生物科技有限公司 | Edible fungus culture medium and preparation method thereof |
| CN107353061A (en) * | 2017-07-26 | 2017-11-17 | 兰溪市奥而特农业科技有限公司 | A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing |
| CN107586723A (en) * | 2017-07-26 | 2018-01-16 | 浦江县欧立生物技术有限公司 | The cultural method of flammulina velutipes liquid strains |
| CN108703312A (en) * | 2018-05-25 | 2018-10-26 | 广东日可威富硒食品有限公司 | A kind of vegetable nutrient reconciles noodles and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103922851A (en) | Black fungus liquid medium and black fungus cultural method | |
| CN102786333B (en) | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same | |
| CN102150555B (en) | Method for culturing pleurotus geesteranus uniformly rich in selenium by using mixed selenium source | |
| CN101948355B (en) | Preparation method of culture medium of edible fungi | |
| CN104823709A (en) | Cultivation method of oyster mushrooms based on mixed compost | |
| CN104987156B (en) | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran | |
| CN105684727B (en) | The culture medium and cultural method for the black collybia albuminosa of cultivation expected based on ferment bacteria residue | |
| CN105110841B (en) | Culture base-material using Lenlinus edodes slag for cultivating hickory chick and preparation method thereof | |
| CN105191667A (en) | Morchella nutrition formula, nutrition bag, preparation method of nutrition bag and morchella culture method | |
| CN103387463A (en) | Cultivation method and cultivation culture material of black fungus | |
| CN105110961A (en) | Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same | |
| CN103583236A (en) | Method for preparing fleckedflesh polypore mycelium | |
| CN107360858A (en) | A kind of breeding method of mushroom edible mushroom | |
| CN101830758B (en) | Edible fungus culture medium | |
| CN104671996A (en) | Special culture medium for straw mushrooms | |
| CN102577839B (en) | Method for producing medicinal or medicine-food dual-purpose fruit body by utilizing yellow wine grains as solid culture medium | |
| CN103583216A (en) | Method for cultivating high-selenium corn and method for preparing nutrition powder of high-selenium corn | |
| CN105284423A (en) | Method for cultivating black fungus with crop straw | |
| CN104591889A (en) | Selenium-enriched oyster mushroom cultivation material and preparation method thereof | |
| CN103005437B (en) | Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation | |
| CN103030452A (en) | Method for preparing liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris | |
| CN107285859A (en) | A kind of cultural method of Hericium erinaceus | |
| CN103553820A (en) | Hypsizigus marmoreus cultivation material using cinnamomum longepaniculatum leaves as raw materials and preparation method thereof | |
| CN103907907B (en) | Selenium-enriching germinated rice bone sauce and preparation method thereof | |
| CN103283480B (en) | Method for growing cordyceps sinensis fruiting body directly in liquid medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140716 |