CN104823709A - Cultivation method of oyster mushrooms based on mixed compost - Google Patents

Cultivation method of oyster mushrooms based on mixed compost Download PDF

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Publication number
CN104823709A
CN104823709A CN201510192765.4A CN201510192765A CN104823709A CN 104823709 A CN104823709 A CN 104823709A CN 201510192765 A CN201510192765 A CN 201510192765A CN 104823709 A CN104823709 A CN 104823709A
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mushroom
medium
cultivation
wheat
inoculation
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王明义
王肇生
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WUZHONG XUKOU JINGYI BIOLOGICAL MEDICINE INSTITUTE
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WUZHONG XUKOU JINGYI BIOLOGICAL MEDICINE INSTITUTE
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Abstract

The invention discloses a cultivation method of oyster mushrooms based on mixed compost. The cultivation method of oyster mushrooms comprises following steps of producing mother seeds, producing basic seeds, preparing culture media of cultivation bags, loading bags, sterilizing, inoculating, cultivating cultivation bags, management of producing mushrooms and timely harvesting. The cultivation method of oyster mushrooms based on mixed compost has following beneficial effects: the method is simple and practicable at low cost; cultivated oyster mushrooms have fine quality and short production period; and high productivity is obtained.

Description

A kind of mushroom cultivation method based on Mixed culture material
Technical field
The invention belongs to fungus growing technique field, relate to a kind of mushroom cultivation method, be specifically related to a kind of method of Mixed culture material cultivating white mushroom.
Background technology
Edible mushroom is of high nutritive value with it, delicious flavour, low in calories and good health-care effect and the treasure be considered as by people in food, have the laudatory title of mountain delicacy delicacies, God's food; Amino acid also containing A wide selection of colours and designs, abundant vitamin and the various enzymes useful to human body; Nearly all mushroom class all contains 8 kinds of essential amino acids that human body self can not synthesize; Edible mushroom is also containing the various bioactivators such as polysaccharide, polypeptide, there is good value medical health care, often edible have promote human metabolism, build up health, improve body immunity, reduce the effect such as cholesterol and blood sugar, especially contained polysaccharide has antitumor, anti-ageing, antiviral, antifatigue, the effect such as hypoglycemic, cause the great interest of biologist, pharmacy man, chemists, therefore food (medicine) is also become increasingly active with the research of bacterium pharmacological action.
Along with the raising of people's living standard, the enhancing of health perception, also increasing to the demand of edible mushroom, and the raw material producing edible mushroom use are industrial or agricultural by product mostly, cheap, wide material sources; Therefore, development mushroom industry, not only can meet the demand of people, and can protection of the environment, the ecological agriculture that turns waste into wealth, promotes Development Technology.
Flat mushroom is one of maximum edible mushroom of China's cultivation amount and consumption figure; Along with flat mushroom production scale expands year by year, composts or fertilisers of cultivating day is becoming tight, and is badly in need of the new raw material of exploitation and meets flat mushroom Production requirement; The different times water content that different material is grown at mushroom growth is all different with pH value; Composts or fertilisers of cultivating all has considerable influence to hypha of Pleurotus ostreatus growing way, long speed, biological efficiency, and selecting suitable composts or fertilisers of cultivating to carry out cultivating white mushroom becomes the technical problem that those skilled in the art need solution badly.
Summary of the invention
Technical problem to be solved by this invention is, for the shortcoming that above prior art exists, propose a kind of mushroom cultivation method based on Mixed culture material, this cultivation method is simple, with low cost, and the flat mushroom quality cultivated is good, with short production cycle, and output is high.
The technical scheme that the present invention solves above technical problem is:
Based on a mushroom cultivation method for Mixed culture material, this mushroom cultivation method flow is: the preparation-pack of the making-cultivation bag medium of the making-cultivated species of female making-original seed of planting, sterilizing-inoculation-bacterium bag cultivation-management of producing mushroom-timely collecting, wherein:
(1) female making of planting, at 120 DEG C, slant medium is planted to the mother prepared and carry out sterilizing 10min, then be aseptically inoculated in by the bacterial strain of flat mushroom on female kind slant medium, then cultivate at 20 DEG C and obtain female kind, mother plants slant medium and comprises following component according to the mass fraction:
Potato: 55 parts, glucose: 5 parts, agar: 8 parts;
Female preparation of planting slant medium is specially: boiled by potato to thoroughly well cooked but not mushy, filter with four layers of gauze, get juice, carry out quantitatively with the water of 1000ml, then agar is added until completely melted, add glucose again, packing test tube, with polypropylene film, test tube mouth is carried out wrapping sealing and obtain female kind slant medium;
(2) making of original seed, plants mother after sterilizing terminates and is aseptically seeded on pedigree seed culture medium, cultivate and obtain original seed at 21 DEG C;
Pedigree seed culture medium is mixed by the wheat of 96% and the gypsum of 2% by mass percentage; Specifically be prepared as: take wheat, then wheat carried out rinsing and reject impurity, soak 10-13h, precook, wheat thoroughly well cooked but not mushy to wheat without white core time, drain away the water, add gypsum, load in the wide-mouth bottle of 750ml after stirring, at distance wide-mouth bottle bottleneck 4cm place, tampon beyond the Great Wall, carries out autoclaving 1h at 127 DEG C, shaking flask while hot after sterilizing terminates, loosen wheat, obtain pedigree seed culture medium;
(3) preparation of cultivation bag medium, cultivation bag medium comprises following component according to the mass fraction:
Cotton seed hulls: 45 parts, beanstalk: 30 parts, wheat bran: 18 parts, sugar: 1 part, calcium carbonate: 2 parts; The preparation of cultivation bag medium is specially:
Be that 1:1.3 adds water and stirs by cotton seed hulls according to material-water ratio, then add beanstalk, wheat bran, sugar and calcium carbonate respectively, then stir 13min, after stirring, obtain the preparation of cultivation bag medium;
(4) pack, sterilizing, the polyethylene plastic bag of cultivation bag medium 22cm × 45cm × 0.004cm is packed, be that the sealing newspaper of 10cm × 10cm and rubber band carry out tying to plastic sack by specification, then the plastic sack after tying is put into Turnround basket and carry out normal-pressure sterilization, sterilising temp controls at 100 DEG C, sterilizing 20h;
(5) inoculate, after sterilizing terminates, bacterial classification plastic sack is put into the cooling of inoculation Indoor Natural, when being cooled to 30 DEG C, the sodium dichloro cyanurate fumigation of every cubic metre of use 10g, then starts inoculation;
The concrete operations of inoculation are: before inoculation, operating personnel use cotton ball soaked in alcohol wiping arm, inoculating tool and cultivated species, during inoculation, and two people one group operations, people's sealing, another people accesses cultivated species, and controlling inoculum concentration is 5%;
(6) bacterial classification plastic bag cultivation, after inoculation terminates, bacterial classification plastic sack is transported to culturing room, and the temperature that controls environment is 25 DEG C, early carries out under subdued light conditions sending out a bacterium, makes regular check on and reject the bacterial classification plastic sack of pollution, and is transported in time outside culturing room and processes;
(7) management of producing mushroom, after a bacterium is terminated, the bacterial classification plastic sack selecting growing way consistent is transported to fruiting canopy, bag weaved on the pad of mushroom canopy ground, by bacterial classification plastic sack pendulum thereon, remove the sealing newspaper on bacterial classification plastic sack, and flower bud is urged in the scattered light stimulation giving 500lx, sprays water, ventilates after there is mushroom flower bud;
(8) gather, mushroom carpophore cap edge is involute, and real in stem, feel is real close, and color, by deeply shoaling gradually, is gathered before not launching spore in time, lives mushroom carpophore when gathering with hand rest, and rotation carries a little medium, change of tide fruiting gently.
The technical scheme that the present invention limits further is:
Aforementioned based in the mushroom cultivation method of Mixed culture material, cotton seed hulls is middle long wool, free from admixture; Soya bean stem meal is broken into long 2cm, the particle of wide 0.2cm.
Aforementioned based in the mushroom cultivation method of Mixed culture material, before step (5) carries out disinfection to transfer room when inoculating, by inoculating tool, cultivated species, cotton ball soaked in alcohol, sterilized newspaper and rubber band are together put into transfer room and are carried out disinfection.
The invention has the beneficial effects as follows:
The present invention is when preparing cultivation bag medium, add cotton seed hulls, cotton seed hulls is the seed coat of lint cottonseed, account for 32% ~ 40% of cottonseed weight, the offcuts of Oil processing factory, cotton seed hulls is nutritious (containing crude protein 7.3%, crude fat 3.5%, raw fiber 37.0%, soluble-carbohydrate 35.0%, coarse ash 3.5%), C/N ratio (23:1) is suitable for, and quality is loosened, water imbibition is strong, particle size is moderate, steady quality, there is good physical proterties, do not harden after adding water, good permeability, the quality raw materials of mushroom cultivation, be called as " omnipotent medium ".
Beanstalk is cauline leaf remaining after pulses leguminous plants threshing, its nutritious (moisture content 14.14%, raw fiber 36.42%, nitrogen free extract 34.21%, crude protein 8.51%, crude fat 1.7%, coarse ash 4.36%, calcium 0.915%, phosphorus 0.21%, potassium 0.482%, sodium 0.048%, magnesium 0.212%, iron 0.067%, zinc 0.048%, copper 7.2 ㎎/㎏, manganese 29.2 ㎎/㎏), China produces beanstalk per year and reaches more than 1,000 ten thousand tons, except small part is used for rural area feed and heating, major part lacks effectively utilization, cause the wasting of resources, the present invention encourages beanstalk and cotton seed hulls with the use of producing edible mushroom, living resources can be made full use of, the scale realizing crops straw transforms, reduce costs, increase the benefit, reduce environmental pollution simultaneously, add appropriate beanstalk in cotton seed hulls can promote Growth of Pleurotus Mycelium, improve biological efficiency.
Embodiment
embodiment 1
The present embodiment provides a kind of mushroom cultivation method based on Mixed culture material, this mushroom cultivation method flow is: the preparation-pack of the making-cultivation bag medium of the making-cultivated species of female making-original seed of planting, sterilizing-inoculation-bacterium bag cultivation-management of producing mushroom-timely collecting, wherein:
(1) female making of planting, at 120 DEG C, slant medium is planted to the mother prepared and carry out sterilizing 10min, then be aseptically inoculated in by the bacterial strain of flat mushroom on female kind slant medium, then cultivate at 20 DEG C and obtain female kind, mother plants slant medium and comprises following component according to the mass fraction:
Potato: 55 parts, glucose: 5 parts, agar: 8 parts;
Female preparation of planting slant medium is specially: boiled by potato to thoroughly well cooked but not mushy, filter with four layers of gauze, get juice, carry out quantitatively with the water of 1000ml, then agar is added until completely melted, add glucose again, packing test tube, with polypropylene film, test tube mouth is carried out wrapping sealing and obtain female kind slant medium;
(2) making of original seed, plants mother after sterilizing terminates and is aseptically seeded on pedigree seed culture medium, cultivate and obtain original seed at 21 DEG C;
Pedigree seed culture medium is mixed by the wheat of 96% and the gypsum of 2% by mass percentage; Specifically be prepared as: take wheat, then wheat carried out rinsing and reject impurity, soak 10h, precook, wheat thoroughly well cooked but not mushy to wheat without white core time, drain away the water, add gypsum, load in the wide-mouth bottle of 750ml after stirring, at distance wide-mouth bottle bottleneck 4cm place, tampon beyond the Great Wall, carries out autoclaving 1h at 127 DEG C, shaking flask while hot after sterilizing terminates, loosen wheat, obtain pedigree seed culture medium;
(3) preparation of cultivation bag medium, cultivation bag medium comprises following component according to the mass fraction:
Cotton seed hulls: 45 parts, beanstalk: 30 parts, wheat bran: 18 parts, sugar: 1 part, calcium carbonate: 2 parts; The preparation of cultivation bag medium is specially:
Be that 1:1.3 adds water and stirs by cotton seed hulls according to material-water ratio, then add beanstalk, wheat bran, sugar and calcium carbonate respectively, then stir 13min, after stirring, obtain the preparation of cultivation bag medium;
Cotton seed hulls is middle long wool, free from admixture; Soya bean stem meal is broken into long 2cm, the particle of wide 0.2cm;
(4) pack, sterilizing, the polyethylene plastic bag of cultivation bag medium 22cm × 45cm × 0.004cm is packed, be that the sealing newspaper of 10cm × 10cm and rubber band carry out tying to plastic sack by specification, then the plastic sack after tying is put into Turnround basket and carry out normal-pressure sterilization, sterilising temp controls at 100 DEG C, sterilizing 20h;
(5) inoculate, after sterilizing terminates, bacterial classification plastic sack is put into the cooling of inoculation Indoor Natural, when being cooled to 30 DEG C, the sodium dichloro cyanurate fumigation of every cubic metre of use 10g, then starts inoculation;
The concrete operations of inoculation are: before carrying out disinfection to transfer room before inoculation, by inoculating tool, cultivated species, cotton ball soaked in alcohol, sterilized newspaper and rubber band are together put into transfer room and are carried out disinfection, operating personnel use cotton ball soaked in alcohol wiping arm, inoculating tool and cultivated species, during inoculation, two people one group operations, people's sealing, another people accesses cultivated species, and controlling inoculum concentration is 5%;
(6) bacterial classification plastic bag cultivation, after inoculation terminates, bacterial classification plastic sack is transported to culturing room, and the temperature that controls environment is 25 DEG C, early carries out under subdued light conditions sending out a bacterium, makes regular check on and reject the bacterial classification plastic sack of pollution, and is transported in time outside culturing room and processes;
(7) management of producing mushroom, after a bacterium is terminated, the bacterial classification plastic sack selecting growing way consistent is transported to fruiting canopy, bag weaved on the pad of mushroom canopy ground, by bacterial classification plastic sack pendulum thereon, remove the sealing newspaper on bacterial classification plastic sack, and flower bud is urged in the scattered light stimulation giving 500lx, sprays water, ventilates after there is mushroom flower bud;
(8) gather, mushroom carpophore cap edge is involute, and real in stem, feel is real close, and color, by deeply shoaling gradually, is gathered before not launching spore in time, lives mushroom carpophore when gathering with hand rest, and rotation carries a little medium, change of tide fruiting gently.
embodiment 2
The present embodiment provides a kind of mushroom cultivation method based on Mixed culture material, this mushroom cultivation method flow is: the preparation-pack of the making-cultivation bag medium of the making-cultivated species of female making-original seed of planting, sterilizing-inoculation-bacterium bag cultivation-management of producing mushroom-timely collecting, wherein:
(1) female making of planting, at 125 DEG C, slant medium is planted to the mother prepared and carry out sterilizing 15min, then be aseptically inoculated in by the bacterial strain of flat mushroom on female kind slant medium, then cultivate at 21 DEG C and obtain female kind, mother plants slant medium and comprises following component according to the mass fraction:
Potato: 40 parts, glucose: 8 parts, agar: 5 parts;
Female preparation of planting slant medium is specially: boiled by potato to thoroughly well cooked but not mushy, filter with four layers of gauze, get juice, carry out quantitatively with the water of 1000ml, then agar is added until completely melted, add glucose again, packing test tube, with polypropylene film, test tube mouth is carried out wrapping sealing and obtain female kind slant medium;
(2) making of original seed, plants mother after sterilizing terminates and is aseptically seeded on pedigree seed culture medium, cultivate and obtain original seed at 24 DEG C;
Pedigree seed culture medium is mixed by the wheat of 98% and the gypsum of 3% by mass percentage; Specifically be prepared as: take wheat, then wheat carried out rinsing and reject impurity, soak 13h, precook, wheat thoroughly well cooked but not mushy to wheat without white core time, drain away the water, add gypsum, load in the wide-mouth bottle of 750ml after stirring, at distance wide-mouth bottle bottleneck 2cm place, tampon beyond the Great Wall, carries out autoclaving 2h at 128 DEG C, shaking flask while hot after sterilizing terminates, loosen wheat, obtain pedigree seed culture medium;
(3) preparation of cultivation bag medium, cultivation bag medium comprises following component according to the mass fraction:
Cotton seed hulls: 50 parts, beanstalk: 20 parts, wheat bran: 20 parts, sugar: 2 parts, calcium carbonate: 1 part; The preparation of cultivation bag medium is specially:
Be that 1:1.3 adds water and stirs by cotton seed hulls according to material-water ratio, then add beanstalk, wheat bran, sugar and calcium carbonate respectively, then stir 10min, after stirring, obtain the preparation of cultivation bag medium;
Cotton seed hulls is middle long wool, free from admixture; Soya bean stem meal is broken into long 2cm, the particle of wide 0.2cm;
(4) pack, sterilizing, the polyethylene plastic bag of cultivation bag medium 22cm × 45cm × 0.004cm is packed, be that the sealing newspaper of 10cm × 10cm and rubber band carry out tying to plastic sack by specification, then the plastic sack after tying is put into Turnround basket and carry out normal-pressure sterilization, sterilising temp controls at 105 DEG C, sterilizing 15h;
(5) inoculate, after sterilizing terminates, bacterial classification plastic sack is put into the cooling of inoculation Indoor Natural, when being cooled to 25 DEG C, the sodium dichloro cyanurate fumigation of every cubic metre of use 13g, then inoculation is started, before carrying out disinfection to transfer room when inoculating, by inoculating tool, cultivated species, cotton ball soaked in alcohol, sterilized newspaper and rubber band are together put into transfer room and are carried out disinfection;
The concrete operations of inoculation are: before inoculation, operating personnel use cotton ball soaked in alcohol wiping arm, inoculating tool and cultivated species, during inoculation, and two people one group operations, people's sealing, another people accesses cultivated species, and controlling inoculum concentration is 5%;
(6) bacterial classification plastic bag cultivation, after inoculation terminates, bacterial classification plastic sack is transported to culturing room, and the temperature that controls environment is 26 DEG C, early carries out under subdued light conditions sending out a bacterium, makes regular check on and reject the bacterial classification plastic sack of pollution, and is transported in time outside culturing room and processes;
(7) management of producing mushroom, after a bacterium is terminated, the bacterial classification plastic sack selecting growing way consistent is transported to fruiting canopy, bag weaved on the pad of mushroom canopy ground, by bacterial classification plastic sack pendulum thereon, remove the sealing newspaper on bacterial classification plastic sack, and flower bud is urged in the scattered light stimulation giving 510lx, sprays water, ventilates after there is mushroom flower bud;
(8) gather, mushroom carpophore cap edge is involute, and real in stem, feel is real close, and color, by deeply shoaling gradually, is gathered before not launching spore in time, lives mushroom carpophore when gathering with hand rest, and rotation carries a little medium, change of tide fruiting gently.
embodiment 3
The present embodiment provides a kind of mushroom cultivation method based on Mixed culture material, this mushroom cultivation method flow is: the preparation-pack of the making-cultivation bag medium of the making-cultivated species of female making-original seed of planting, sterilizing-inoculation-bacterium bag cultivation-management of producing mushroom-timely collecting, wherein:
(1) female making of planting, at 122 DEG C, slant medium is planted to the mother prepared and carry out sterilizing 12min, then be aseptically inoculated in by the bacterial strain of flat mushroom on female kind slant medium, then cultivate at 23 DEG C and obtain female kind, mother plants slant medium and comprises following component according to the mass fraction:
Potato: 48 parts, glucose: 6 parts, agar: 10 parts;
Female preparation of planting slant medium is specially: boiled by potato to thoroughly well cooked but not mushy, filter with four layers of gauze, get juice, carry out quantitatively with the water of 1000ml, then agar is added until completely melted, add glucose again, packing test tube, with polypropylene film, test tube mouth is carried out wrapping sealing and obtain female kind slant medium;
(2) making of original seed, plants mother after sterilizing terminates and is aseptically seeded on pedigree seed culture medium, cultivate and obtain original seed at 22 DEG C;
Pedigree seed culture medium is mixed by the wheat of 97% and the gypsum of 4% by mass percentage; Specifically be prepared as: take wheat, then wheat carried out rinsing and reject impurity, soak 12h, precook, wheat thoroughly well cooked but not mushy to wheat without white core time, drain away the water, add gypsum, load in the wide-mouth bottle of 750ml after stirring, at distance wide-mouth bottle bottleneck 3cm place, tampon beyond the Great Wall, carries out autoclaving 2h at 126 DEG C, shaking flask while hot after sterilizing terminates, loosen wheat, obtain pedigree seed culture medium;
(3) preparation of cultivation bag medium, cultivation bag medium comprises following component according to the mass fraction:
Cotton seed hulls: 48 parts, beanstalk: 25 parts, wheat bran: 15 parts, sugar: 1 part, calcium carbonate: 3 parts; The preparation of cultivation bag medium is specially:
Be that 1:1.3 adds water and stirs by cotton seed hulls according to material-water ratio, then add beanstalk, wheat bran, sugar and calcium carbonate respectively, then stir 15min, after stirring, obtain the preparation of cultivation bag medium;
Cotton seed hulls is middle long wool, free from admixture; Soya bean stem meal is broken into long 2cm, the particle of wide 0.2cm;
(4) pack, sterilizing, the polyethylene plastic bag of cultivation bag medium 22cm × 45cm × 0.004cm is packed, be that the sealing newspaper of 10cm × 10cm and rubber band carry out tying to plastic sack by specification, then the plastic sack after tying is put into Turnround basket and carry out normal-pressure sterilization, sterilising temp controls at 102 DEG C, sterilizing 18h;
(5) inoculate, after sterilizing terminates, bacterial classification plastic sack is put into the cooling of inoculation Indoor Natural, when being cooled to 20 DEG C, the sodium dichloro cyanurate fumigation of every cubic metre of use 15g, then inoculation is started, before carrying out disinfection to transfer room when inoculating, by inoculating tool, cultivated species, cotton ball soaked in alcohol, sterilized newspaper and rubber band are together put into transfer room and are carried out disinfection;
The concrete operations of inoculation are: before inoculation, operating personnel use cotton ball soaked in alcohol wiping arm, inoculating tool and cultivated species, during inoculation, and two people one group operations, people's sealing, another people accesses cultivated species, and controlling inoculum concentration is 5%;
(6) bacterial classification plastic bag cultivation, after inoculation terminates, bacterial classification plastic sack is transported to culturing room, and the temperature that controls environment is 25 DEG C, early carries out under subdued light conditions sending out a bacterium, makes regular check on and reject the bacterial classification plastic sack of pollution, and is transported in time outside culturing room and processes;
(7) management of producing mushroom, after a bacterium is terminated, the bacterial classification plastic sack selecting growing way consistent is transported to fruiting canopy, bag weaved on the pad of mushroom canopy ground, by bacterial classification plastic sack pendulum thereon, remove the sealing newspaper on bacterial classification plastic sack, and flower bud is urged in the scattered light stimulation giving 505lx, sprays water, ventilates after there is mushroom flower bud;
(8) gather, mushroom carpophore cap edge is involute, and real in stem, feel is real close, and color, by deeply shoaling gradually, is gathered before not launching spore in time, lives mushroom carpophore when gathering with hand rest, and rotation carries a little medium, change of tide fruiting gently.
In addition to the implementation, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of application claims.

Claims (3)

1. the mushroom cultivation method based on Mixed culture material, it is characterized in that, this mushroom cultivation method flow is: the preparation-pack of the making-cultivation bag medium of the making-cultivated species of female making-original seed of planting, sterilizing-inoculation-bacterium bag cultivation-management of producing mushroom-timely collecting, wherein:
(1) female making of planting, at 120-125 DEG C, slant medium is planted to the mother prepared and carry out sterilizing 10-15min, then the bacterial strain of flat mushroom is aseptically inoculated on female kind slant medium, then cultivate at 20-23 DEG C and obtain female kind, described mother plants slant medium and comprises following component according to the mass fraction:
Potato: 40-55 part, glucose: 5-8 part, agar: 5-10 part;
The preparation that described mother plants slant medium is specially: boiled by potato to thoroughly well cooked but not mushy, filter with four layers of gauze, get juice, carry out quantitatively with the water of 1000ml, then agar is added until completely melted, add glucose again, packing test tube, with polypropylene film, test tube mouth is carried out wrapping sealing and obtain female kind slant medium;
(2) making of original seed, plants mother after sterilizing terminates and is aseptically seeded on pedigree seed culture medium, cultivate and obtain original seed at 21-24 DEG C;
Described pedigree seed culture medium is mixed by the wheat of 96-98% and the gypsum of 2-4% by mass percentage; Specifically be prepared as: take wheat, then wheat carried out rinsing and reject impurity, soak 10-13h, precook, wheat thoroughly well cooked but not mushy to wheat without white core time, drain away the water, add gypsum, load in the wide-mouth bottle of 750ml after stirring, at distance wide-mouth bottle bottleneck 2-4cm place, tampon beyond the Great Wall, carries out autoclaving 1-2h at 126-128 DEG C, shaking flask while hot after sterilizing terminates, loosen wheat, obtain pedigree seed culture medium;
(3) preparation of cultivation bag medium, described cultivation bag medium comprises following component according to the mass fraction:
Cotton seed hulls: 45-50 part, beanstalk: 20-30 part, wheat bran: 15-20 part, sugar: 1-2 part, calcium carbonate: 1-3 part; The preparation of described cultivation bag medium is specially:
Be that 1:1.3 adds water and stirs by cotton seed hulls according to material-water ratio, then add beanstalk, wheat bran, sugar and calcium carbonate respectively, then stir 10-15min, after stirring, obtain the preparation of cultivation bag medium;
(4) pack, sterilizing, the polyethylene plastic bag of cultivation bag medium 22cm × 45cm × 0.004cm is packed, be that the sealing newspaper of 10cm × 10cm and rubber band carry out tying to plastic sack by specification, then the plastic sack after tying is put into Turnround basket and carry out normal-pressure sterilization, sterilising temp controls at 100-105 DEG C, sterilizing 15-20h;
(5) inoculate, after sterilizing terminates, bacterial classification plastic sack is put into the cooling of inoculation Indoor Natural, when being cooled to 20-30 DEG C, the sodium dichloro cyanurate fumigation of every cubic metre of use 10-15g, then starts inoculation;
The concrete operations of described inoculation are: before inoculation, and operating personnel use cotton ball soaked in alcohol wiping arm, inoculating tool and cultivated species, during inoculation, and two people one group operations, people's sealing, another people accesses cultivated species, and controlling inoculum concentration is 5%;
(6) bacterial classification plastic bag cultivation, after inoculation terminates, bacterial classification plastic sack is transported to culturing room, and the temperature that controls environment is 25-26 DEG C, early carries out under subdued light conditions sending out a bacterium, makes regular check on and reject the bacterial classification plastic sack of pollution, and is transported in time outside culturing room and processes;
(7) management of producing mushroom, send out after bacterium terminates, the bacterial classification plastic sack selecting growing way consistent is transported to fruiting canopy, bag weaved on the pad of mushroom canopy ground, by bacterial classification plastic sack pendulum thereon, remove the sealing newspaper on bacterial classification plastic sack, and flower bud is urged in the scattered light stimulation giving 500-510lx, sprays water, ventilates after there is mushroom flower bud;
(8) gather, mushroom carpophore cap edge is involute, and real in stem, feel is real close, and color, by deeply shoaling gradually, is gathered before not launching spore in time, lives mushroom carpophore when gathering with hand rest, and rotation carries a little medium, change of tide fruiting gently.
2. the mushroom cultivation method based on Mixed culture material according to claim 1, is characterized in that: described cotton seed hulls is middle long wool, free from admixture; Described soya bean stem meal is broken into long 2cm, the particle of wide 0.2cm.
3. the mushroom cultivation method based on Mixed culture material according to claim 1, it is characterized in that: before step (5) carries out disinfection to transfer room when inoculating, by inoculating tool, cultivated species, cotton ball soaked in alcohol, sterilized newspaper and rubber band are together put into transfer room and are carried out disinfection.
CN201510192765.4A 2015-04-22 2015-04-22 Cultivation method of oyster mushrooms based on mixed compost Pending CN104823709A (en)

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CN105103961A (en) * 2015-09-14 2015-12-02 河南农业大学 Method for increasing yield of edible mushrooms by means of methylation inhibitor
CN106431658A (en) * 2016-09-28 2017-02-22 长江师范学院 Oyster mushroom culture medium and oyster mushroom cultivation method
CN107094498A (en) * 2017-06-07 2017-08-29 河南省农业科学院植物营养与资源环境研究所 A kind of batch production pocket type cultivation method of flat mushroom
CN107475136A (en) * 2017-09-30 2017-12-15 山东省农业科学院农业资源与环境研究所 A kind of special mother culture media of flat mushroom and preparation method and application
CN107517731A (en) * 2017-09-26 2017-12-29 界首市海棠湾生态农业科技有限公司 A kind of cultural method of high nutrition quality flat mushroom
CN107736190A (en) * 2017-11-13 2018-02-27 云南菌视界生物科技有限公司 A kind of waste material after fruiting using schizophyllum commune cultivates the method that high warm lung shape is picked up the ears
CN107821004A (en) * 2017-10-26 2018-03-23 贵州大学 A kind of culture medium and its cultural method using the slag for cultivating elm mushroom that ends
CN107828664A (en) * 2017-10-25 2018-03-23 湖南省农业生物技术研究中心 Schizophyllum commune XT 1 and its cultural method and application
CN107873391A (en) * 2017-10-26 2018-04-06 贵州大学 The micro-organisms base and its cultural method of a kind of fungus cultivation
CN107950288A (en) * 2017-11-20 2018-04-24 山东省农业科学院农业资源与环境研究所 A kind of planting technique of straw mushroom
CN107996286A (en) * 2017-10-26 2018-05-08 贵州大学 A kind of Chinese mugwort slag culture medium and its cultural method for reducing Pleurotus.cornucopiae(Paul.ex Pers.)Rolland abnormal rate
CN108002877A (en) * 2017-10-26 2018-05-08 贵州大学 A kind of culture medium and its cultural method using the slag for cultivating oyster mushroom that ends
CN108076963A (en) * 2017-10-26 2018-05-29 贵州大学 A kind of red sesame culture medium and preparation method thereof
CN108207485A (en) * 2017-12-29 2018-06-29 凤台县鼎足农业发展有限公司 It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method
CN109511465A (en) * 2018-11-27 2019-03-26 胡俊凯 A kind of oyster mushroom cultivating in bag method based on corncob
CN113951049A (en) * 2021-09-03 2022-01-21 广西南亚热带农业科学研究所 Culture medium taking macadimia nut peels as raw materials and method for cultivating oyster mushrooms by using culture medium
CN114788476A (en) * 2022-04-14 2022-07-26 河南农业大学 Cultivation method of transgenic oyster mushroom Pofst3+

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CN105027986B (en) * 2015-08-25 2018-06-29 贵州茅台流域东升农场有限公司 Packed cultural method in a kind of oyster mushroom room
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CN107517731A (en) * 2017-09-26 2017-12-29 界首市海棠湾生态农业科技有限公司 A kind of cultural method of high nutrition quality flat mushroom
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CN107828664A (en) * 2017-10-25 2018-03-23 湖南省农业生物技术研究中心 Schizophyllum commune XT 1 and its cultural method and application
CN107828664B (en) * 2017-10-25 2020-10-23 湖南省农业生物技术研究中心 Schizophyllum commune XT-1 and cultivation method and application thereof
CN107873391A (en) * 2017-10-26 2018-04-06 贵州大学 The micro-organisms base and its cultural method of a kind of fungus cultivation
CN107821004A (en) * 2017-10-26 2018-03-23 贵州大学 A kind of culture medium and its cultural method using the slag for cultivating elm mushroom that ends
CN107996286A (en) * 2017-10-26 2018-05-08 贵州大学 A kind of Chinese mugwort slag culture medium and its cultural method for reducing Pleurotus.cornucopiae(Paul.ex Pers.)Rolland abnormal rate
CN108002877A (en) * 2017-10-26 2018-05-08 贵州大学 A kind of culture medium and its cultural method using the slag for cultivating oyster mushroom that ends
CN108076963A (en) * 2017-10-26 2018-05-29 贵州大学 A kind of red sesame culture medium and preparation method thereof
CN108002877B (en) * 2017-10-26 2021-10-01 贵州大学 Culture medium for cultivating oyster mushroom by using moxa dregs and cultivation method thereof
CN107736190A (en) * 2017-11-13 2018-02-27 云南菌视界生物科技有限公司 A kind of waste material after fruiting using schizophyllum commune cultivates the method that high warm lung shape is picked up the ears
CN107950288A (en) * 2017-11-20 2018-04-24 山东省农业科学院农业资源与环境研究所 A kind of planting technique of straw mushroom
CN107950288B (en) * 2017-11-20 2020-08-21 山东省农业科学院农业资源与环境研究所 Straw mushroom cultivation process
CN108207485A (en) * 2017-12-29 2018-06-29 凤台县鼎足农业发展有限公司 It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method
CN109511465A (en) * 2018-11-27 2019-03-26 胡俊凯 A kind of oyster mushroom cultivating in bag method based on corncob
CN113951049A (en) * 2021-09-03 2022-01-21 广西南亚热带农业科学研究所 Culture medium taking macadimia nut peels as raw materials and method for cultivating oyster mushrooms by using culture medium
CN114788476A (en) * 2022-04-14 2022-07-26 河南农业大学 Cultivation method of transgenic oyster mushroom Pofst3+

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