CN104115668A - Production method for oyster mushrooms rich in selenium and zinc - Google Patents
Production method for oyster mushrooms rich in selenium and zinc Download PDFInfo
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
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- 239000011669 selenium Substances 0.000 title abstract description 22
- 229910052711 selenium Inorganic materials 0.000 title abstract description 22
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 title abstract description 12
- 239000011701 zinc Substances 0.000 title abstract description 12
- 229910052725 zinc Inorganic materials 0.000 title abstract description 12
- 235000001603 Pleurotus ostreatus Nutrition 0.000 title abstract 5
- 240000001462 Pleurotus ostreatus Species 0.000 title abstract 5
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 24
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 24
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 24
- 239000011781 sodium selenite Substances 0.000 claims abstract description 24
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 22
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 15
- 238000002360 preparation method Methods 0.000 claims abstract description 14
- 239000002361 compost Substances 0.000 claims abstract description 11
- 239000001963 growth medium Substances 0.000 claims abstract description 8
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 33
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 24
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 claims description 23
- 235000009529 zinc sulphate Nutrition 0.000 claims description 20
- 239000011686 zinc sulphate Substances 0.000 claims description 20
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- 229920001817 Agar Polymers 0.000 claims description 12
- 244000061456 Solanum tuberosum Species 0.000 claims description 12
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 12
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 9
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 239000001888 Peptone Substances 0.000 claims description 8
- 108010080698 Peptones Proteins 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 8
- 235000019319 peptone Nutrition 0.000 claims description 8
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 8
- 235000015099 wheat brans Nutrition 0.000 claims description 8
- 229930003451 Vitamin B1 Natural products 0.000 claims description 7
- 229960003495 thiamine Drugs 0.000 claims description 7
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 7
- 239000011691 vitamin B1 Substances 0.000 claims description 7
- 235000010374 vitamin B1 Nutrition 0.000 claims description 7
- 241000196324 Embryophyta Species 0.000 claims description 6
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- 235000012255 calcium oxide Nutrition 0.000 claims description 4
- PASHVRUKOFIRIK-UHFFFAOYSA-L calcium sulfate dihydrate Chemical compound O.O.[Ca+2].[O-]S([O-])(=O)=O PASHVRUKOFIRIK-UHFFFAOYSA-L 0.000 claims description 4
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
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- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 4
- 239000010440 gypsum Substances 0.000 claims description 4
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- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a production method for oyster mushrooms rich in selenium and zinc, and belongs to the technical field of cultivation of edible fungi. The production method comprises the steps of mother strain preparation, stock preparation and cultivation of oyster mushrooms, wherein sodium selenite and zinc sulfate are added into a mother strain preparation culture medium and a stock preparation culture medium, and sodium selenite and zinc sulfate are further added to composts for oyster mushroom cultivation. By means of the production method, the oyster mushrooms absorb a high content of selenium and zinc in the growing process, are high in yield, and can well provide and add the selenium and zinc microelements needed by the human body when serving as food.
Description
Technical field
The present invention relates to a kind of flat mushroom production method, particularly a kind of flat mushroom production method that is rich in selenium zinc, belongs to fungus growing technique field.
Background technology
Selenium, zinc are the trace elements of needed by human, lack selenium and can significantly reduce body immunity, lack selenium area endemic and the cardiovascular and cerebrovascular diseases such as Keshan disease, Kaschin-Beck disease easily occur.Appropriate supplement selenium has the effect of remarkable improvement to cancer, cataract, childhood dystrophy, and can avoid cardiovascular and cerebrovascular wall generation fiber pathology and the generation of the diseases such as the arteriosclerosis that causes, hypertension, miocardial infarction, senile nervous function are complete, memory and dysnoesia.Mend selenium and can eliminate interior free yl, reduce WBV, plasma viscosity; Mend the favourable promotion upgrowth and development of children of selenium; The elderly is mended to selenium and can prevent and treat the diseases such as arteriosclerosis, cerebral thrombus; Mend selenium cancer and the leukemia of the organs such as lung, liver, colon, prostate, mammary gland are all had to obvious prevention and cure effect.Infant lacks zinc not only can cause the stagnation of growing, and can affect the growth of infant intelligence.If lack zinc fetus and infantile stage, also can cause intelligence development obstacle.In addition, if experiment showed,, parent lacks zinc in the pregnancy period, and deformity may appear in its baby who gives birth to, and occurs the change of nervous function.Reasonably supplement zinc, can make leucocyte effect strengthen, adjust the immunologic derangement bringing because of physiological ageing, bring into play best immunologic function.Simultaneously, zinc supplementation also can weaken cytotoxic murder by poisoning, human body is resisted to infect and play an important role, approximately there is the scarce selenium of 72% degree from different places in China, particularly some areas of 15 provinces, cities and autonomous regions from northeast to southwest have formed " poor selenium area ", also have Soviet Union, Anhui, Shandong, sweet, peaceful etc. economize, also relatively poor selenium of municipal some areas, start to develop and produce selenium-enriched food, manually adding selenium and produce rich selenium food, is to solve the effective ways that lack selenium problem.There is in the market multiple se-rich health food products, but the price-pegging that it is comparatively expensive the consumer group, and be difficult to be accepted by vast ordinary consumer, in edible mushroom, flat mushroom is that one is accepted extensively and liked by masses, the flat mushroom that selenium zinc is rich in cultivation not only can make the trace element of human body be supplemented, and can improve the added value of cultivating edible, therefore, exploiting economy, convenience, be applicable to directly edible mushroom with abundant selenium product and have broad prospects.
Summary of the invention
The present invention is for providing a kind of flat mushroom production method that is rich in selenium zinc.
A kind of flat mushroom production method that is rich in selenium zinc provided by the present invention, comprise prepared by female kind, original seed preparation, mushroom cultivation, plant and prepare medium mother, original seed is prepared and in culture medium prescription, is all added with sodium selenite and zinc sulphate, in mushroom cultivation composts or fertilisers of cultivating, be added with sodium selenite and zinc sulphate, described mother's kind prepares culture medium prescription composition and weight ratio is: potato: glucose: agar: peptone: potassium dihydrogen phosphate: magnesium sulfate: vitamin B1: sodium selenite: white vitriol: water=200g:20g:20-25g:1-3g:0.5-1.5g:0.5-1.5g:0.5-1.5g:5-10mg, 0.1-0.2g:1000g, described mother plants preparation method: after going eye section to boil by the peeling potatoes of said ratio, obtain potato juice, put into agar, after dissolving, agar adds glucose by described consumption, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1, sodium selenite, water, make medium, packing test tube carries out autoclaving, at pressure 0.05MPa, step-down back to zero when 105 DEG C of temperature, Pressurized-heated is to 0.14MPa again, 120 DEG C maintain 30 minutes step-down back to zeros, when being down to 50 DEG C, temperature takes out, when temperature is down to 30 DEG C, the bacterial classification of transferring under aseptic condition, through 25 DEG C of constant temperature culture 15 days, both female kind, described original seed prepares culture material formula and weight ratio is: wood chip: wheat bran: magnesium sulfate: gypsum: sodium selenite: white vitriol: water=780g:200g:10g:10g:20mg-30mg:0.2g-0.5g:1000g-1200g, the preparation method that described original seed is prepared composts or fertilisers of cultivating is: the composts or fertilisers of cultivating in formula is above mixed thoroughly and point installed in seed bottle, seal sterilizing with tampon, at pressure 0.05MPa, step-down back to zero when 105 DEG C of temperature, Pressurized-heated is to 0.14MPa again, 120 DEG C maintain 30 minutes step-down back to zeros, temperature is down to 30 DEG C when following, under aseptic condition, access is female, 25 DEG C of unglazed cultivations 40 days, cover with bottle and be original seed, described mushroom cultivation culture material formula and weight ratio are: corncob: wheat bran or corn flour: land plaster: superphosphate: quicklime: sodium selenite: white vitriol: water=84kg:14kg:0.5-1kg:0.5kg:1.5kg:5-10mg:0.3-0.5g:100-120kg.
A kind of flat mushroom production method that is rich in selenium zinc provided by the present invention, in mother, directly import selenium zinc with original seed preparatory phase, in process of growth, selenium zinc absorbs high, output is high, the cultivation of rich selenium zinc flat mushroom of the present invention, can greatly reduce living contaminants rate, improve product yield 10%, the ratio of first-grade products increases by 20%, realize selenium zinc content in mushroom body and reach 100-200 mg/1000g dry weight, content increases more than 500 times compared with general mushroom, can solve preferably and make up the selenium zinc microelement of needed by human body as food.
Embodiment
In order to explain more fully enforcement of the present invention, embodiment of the present invention is provided, these embodiments are only to elaboration of the present invention, do not limit the scope of the invention.The material name adopting in the present invention, for material be that industry is known, the mode that adds of zinc sulphate of the present invention adopts white vitriol.
A kind of flat mushroom production method that is rich in selenium zinc provided by the present invention, comprise prepared by female kind, original seed preparation, mushroom cultivation, plant and prepare medium mother, original seed is prepared and in culture medium prescription, is all added with sodium selenite and zinc sulphate, in mushroom cultivation composts or fertilisers of cultivating, be added with sodium selenite and zinc sulphate, described mother's kind prepares culture medium prescription composition and weight ratio is: potato: glucose: agar: peptone: potassium dihydrogen phosphate: magnesium sulfate: vitamin B1: sodium selenite: white vitriol: water=200g:20g:20-25g:1-3g:0.5-1.5g:0.5-1.5g:0.5-1.5g:5-10mg, 0.1-0.2g:1000g, described mother plants preparation method: after going eye section to boil by the peeling potatoes of said ratio, obtain potato juice, put again agar, after dissolving, agar adds glucose by described consumption, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1, sodium selenite, water, make medium, packing test tube carries out autoclaving, step-down back to zero in the time of 105 DEG C of pressure 0.05MPa temperature, Pressurized-heated is to 0.14MPa again, 120 DEG C maintain 30 minutes step-down back to zeros, when being down to 50 DEG C, temperature takes out, when temperature is down to 30 DEG C, the bacterial classification of transferring under aseptic condition, through 25 DEG C of constant temperature culture 15 days, both female kind, described original seed prepares culture material formula and weight ratio is: wood chip: wheat bran: magnesium sulfate: gypsum: sodium selenite: white vitriol: water=780g:200g:10g:10g:20mg-30mg:0.2g-0.5g:1000g-1200g, the preparation method that described original seed is prepared composts or fertilisers of cultivating is: the composts or fertilisers of cultivating in formula is above mixed thoroughly and point installed in seed bottle, seal sterilizing with tampon, at pressure 0.05MPa, step-down back to zero when 105 DEG C of temperature, Pressurized-heated is to 0.14MPa again, 120 DEG C maintain 30 minutes step-down back to zeros, temperature is down to 30 DEG C when following, under aseptic condition, access is female, 25 DEG C of unglazed cultivations 40 days, cover with bottle and be original seed, described mushroom cultivation culture material formula and weight ratio are: corncob: wheat bran or corn flour: land plaster: superphosphate: quicklime: sodium selenite: white vitriol: water=84kg:14kg:0.5-1kg:0.5kg:1.5kg:5-10mg:0.3-0.5g:100-120kg.
Further embodiment is as follows:
1) prepared by female kind
Formula: potato 200g, glucose 20g, agar 20g, peptone 2g, potassium dihydrogen phosphate 1g, magnesium sulfate 1g, VB11 g, sodium selenite 8mg, white vitriol 0.2g, water 1000 mL, peeling potatoes is removed to eye, and section is boiled, and obtains potato juice, put again agar, after agar dissolves, add glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1, sodium selenite, water by described consumption, make 1000ml medium, packing test tube carries out autoclaving.Steam bleeding step-down back to zero when 105 DEG C of pressure 0.05MPa, temperature, Pressurized-heated maintains 30 minutes steam bleeding step-down back to zeros to 0.14MPa, 120 DEG C again, taking out test tube when temperature is down to 50 DEG C in autoclave, put while hot, make medium become inclined-plane, when temperature is down to 30 DEG C, the bacterial classification of transferring under aseptic condition, through 25 DEG C of constant temperature culture 15 days, covers with inclined-plane and is female kind;
2) original seed preparation
Formula: wood chip 780g, wheat bran 200g, magnesium sulfate 10g, gypsum 10g, sodium selenite 226mg, white vitriol 0.4g, water 1100g,
[0010]preparation method: the composts or fertilisers of cultivating in formula is above mixed thoroughly and point installed in seed bottle, seal sterilizing with tampon, steam bleeding step-down back to zero when 105 DEG C of pressure 0.05MPa, temperature, Pressurized-heated maintains 30 minutes steam bleeding step-down back to zeros to 0.14MPa, 120 DEG C again, temperature is down to 30 DEG C when following, and under aseptic condition, access is female plants, every female switching 5-8 bottle original seed of planting, 25 DEG C of unglazed cultivations 40 days, cover with bottle and are original seed;
3) the rich zinc mushroom cultivation of rich selenium
Culture material formula: corncob 84kg, wheat bran or corn flour 14 kg, land plaster 0.8 kg, superphosphate 0.5 kg, quicklime 1.5 kg, sodium selenite 10mg, white vitriol 0.5g, water 112kg, bacterium bag adopts polyethylene bagging, normal-pressure sterilization, temperature reaches 100 DEG C and continues 48 hours, after taking the dish out of the pot, temperature is down to 30 DEG C when following, under aseptic condition, access original seed, be and produce bacterium bag;
4) management: the planting type that adopts three-dimensional code bag, two fruiting, the high 5-8 layer of code, send out bacterium stage regulating and controlling temperature in 25 DEG C of left and right, airtight illumination, appropriate ventilation, air humidity nature, fruiting stage, between 5 DEG C～20 DEG C of regulating and controlling temperatures, intensity of illumination 500Lx left and right, appropriate ventilation, air humidity 95% left and right; The rich zinc flat mushroom of rich selenium is answered timely collecting, is beneficial to improve commodity rate, and deep processing can oven dry, quick-frozen and pickled.
A kind of flat mushroom production method that is rich in selenium zinc provided by the present invention, in mother, directly import selenium zinc with original seed preparatory phase, in process of growth, selenium zinc absorbs high, output is high, and the cultivation of rich selenium zinc flat mushroom of the present invention, can greatly reduce living contaminants rate, improve product yield 10%, the ratio of first-grade products increases by 20%, realizes selenium zinc content in mushroom body and reaches 100-200 mg/100 0g dry weight, and content increases more than 500 times compared with general mushroom.
After describing embodiments of the present invention in detail, the personage who is familiar with this technology can be well understood to, can carry out various variations and amendment not departing under above-mentioned claim and spirit, any simple modification, equivalent variations and modification that all foundations technical spirit of the present invention is done above embodiment, all belong to the scope of technical solution of the present invention, and the present invention is not also limited to the embodiment of example in specification.
Claims (6)
1. one kind is rich in the flat mushroom production method of selenium zinc, comprise that prepared by female kind, prepared by original seed, mushroom cultivation, it is characterized in that: prepare medium, original seed in mother's kind and in preparing culture medium prescription, be all added with sodium selenite and zinc sulphate, in mushroom cultivation composts or fertilisers of cultivating, be added with sodium selenite and zinc sulphate.
2. a kind of flat mushroom production method that is rich in selenium zinc according to claim 1, is characterized in that: described mother's kind prepares culture medium prescription composition and weight ratio is: potato: glucose: agar: peptone: potassium dihydrogen phosphate: magnesium sulfate: vitamin B1: sodium selenite: white vitriol: water=200g:20g:20-25g:1-3g:0.5-1.5g:0.5-1.5g:0.5-1.5g:5-10mg, 0.1-0.2g:1000g.
3. a kind of flat mushroom production method that is rich in selenium zinc according to claim 2, it is characterized in that: described mother plants preparation method and is: after going eye section to boil by the peeling potatoes of said ratio, obtain potato juice, put into agar, after dissolving, agar adds glucose by described consumption, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1, sodium selenite, water, make medium, packing test tube carries out autoclaving, at pressure 0.05MPa, step-down back to zero when 105 DEG C of temperature, Pressurized-heated is to 0.14MPa again, 120 DEG C maintain 30 minutes step-down back to zeros, when being down to 50 DEG C, temperature takes out, when temperature is down to 30 DEG C, the bacterial classification of transferring under aseptic condition, through 25 DEG C of constant temperature culture 15 days, both female kind.
4. a kind of flat mushroom production method that is rich in selenium zinc according to claim 1, is characterized in that: described original seed prepares culture material formula and weight ratio is: wood chip: wheat bran: magnesium sulfate: gypsum: sodium selenite: white vitriol: water=780g:200g:10g:10g:20mg-30mg:0.2g-0.5g:1000g-1200g.
5. a kind of flat mushroom production method that is rich in selenium zinc according to claim 4, it is characterized in that: the preparation method that described original seed is prepared composts or fertilisers of cultivating is: the composts or fertilisers of cultivating in formula is above mixed thoroughly and point installed in seed bottle, seal sterilizing with tampon, step-down back to zero in the time of 105 DEG C of pressure 0.05MPa, temperature, Pressurized-heated maintains 30 minutes step-down back to zeros to 0.14MPa, 120 DEG C again, temperature is down to 30 DEG C when following, under aseptic condition, access is female, 25 DEG C of unglazed cultivations 40 days, cover with bottle and are original seed.
6. a kind of flat mushroom production method that is rich in selenium zinc according to claim 1, is characterized in that: described mushroom cultivation culture material formula and weight ratio are: corncob: wheat bran or corn flour: land plaster: superphosphate: quicklime: sodium selenite: white vitriol: water=84kg:14kg:0.5-1kg:0.5kg:1.5kg:5-10mg:0.3-0.5g:100-120kg.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104641936A (en) * | 2015-01-29 | 2015-05-27 | 谢光玉 | Cultivation method for selenium-enriched oyster mushrooms |
| CN104823709A (en) * | 2015-04-22 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Cultivation method of oyster mushrooms based on mixed compost |
| CN105175154A (en) * | 2015-10-15 | 2015-12-23 | 马永强 | Special culture medium for artificially cultivating pollution-free oyster mushrooms and preparation method of special culture medium |
| CN105237160A (en) * | 2015-09-10 | 2016-01-13 | 凤台县星展食用菌有限公司 | Selenium-rich high-effective oyster mushroom culture medium and preparation method thereof |
| CN105580643A (en) * | 2016-03-09 | 2016-05-18 | 重庆市圣垦农业开发有限公司 | Method for cultivating oyster mushrooms |
| CN105940957A (en) * | 2016-06-08 | 2016-09-21 | 柯淘 | Selenium-rich oyster mushroom planting method |
| CN106134757A (en) * | 2015-04-03 | 2016-11-23 | 池州市德丰菌业有限责任公司 | A kind of zinc-rich selenium mushroom cultivating method |
| CN108207485A (en) * | 2017-12-29 | 2018-06-29 | 凤台县鼎足农业发展有限公司 | It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method |
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| CN104641936A (en) * | 2015-01-29 | 2015-05-27 | 谢光玉 | Cultivation method for selenium-enriched oyster mushrooms |
| CN106134757A (en) * | 2015-04-03 | 2016-11-23 | 池州市德丰菌业有限责任公司 | A kind of zinc-rich selenium mushroom cultivating method |
| CN104823709A (en) * | 2015-04-22 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Cultivation method of oyster mushrooms based on mixed compost |
| CN105237160A (en) * | 2015-09-10 | 2016-01-13 | 凤台县星展食用菌有限公司 | Selenium-rich high-effective oyster mushroom culture medium and preparation method thereof |
| CN105175154A (en) * | 2015-10-15 | 2015-12-23 | 马永强 | Special culture medium for artificially cultivating pollution-free oyster mushrooms and preparation method of special culture medium |
| CN105580643A (en) * | 2016-03-09 | 2016-05-18 | 重庆市圣垦农业开发有限公司 | Method for cultivating oyster mushrooms |
| CN105940957A (en) * | 2016-06-08 | 2016-09-21 | 柯淘 | Selenium-rich oyster mushroom planting method |
| CN108207485A (en) * | 2017-12-29 | 2018-06-29 | 凤台县鼎足农业发展有限公司 | It is a kind of using powdered rice hulls, silkworm excrement as culture material culture oyster mushroom method |
| CN108450236A (en) * | 2018-01-30 | 2018-08-28 | 新平源健农业开发有限公司 | A kind of culture medium and preparation method thereof for flat mushroom strain culture |
| CN109006187A (en) * | 2018-10-18 | 2018-12-18 | 安徽铜草花现代农业科技有限公司 | A kind of packed culture medium of high yield oyster mushroom and preparation method thereof |
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