CN101658102B - Cultivating method of pleurotus ferulae - Google Patents
Cultivating method of pleurotus ferulae Download PDFInfo
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Abstract
The invention relates to a cultivating method of pleurotus ferulae, which takes white lees as cultivated materials in each process of cultivating the original seeds, the cultivated seeds and the inoculated seeds of the pleurotus ferulae. The method adopts the white lees to cultivate the pleurotus ferulae, thereby not only being capable of improving the yield and the quality of the pleurotus ferulae, but also being capable of taking collected mushroom bran as lees mycoprotein materials for the breeding industry, preventing waste materials from polluting environment, and being capable of improving economic benefit by more than 20%; and when house culturing, fresh white lees is taken as the cultivating materials without sterilizing and is directly bagged, thereby simplifying operation procedure, reducing production cost, and having obvious social benefit, economic benefit and ecological benefit.
Description
Technical field
The present invention relates to a kind of cultivation method of Pleuotus nebrodensis Quel, belong to the field of planting of edible fungus mushroom.
Background technology
The continuous understanding of Along with people's growth in the living standard and, nutrition nuisanceless to edible fungus, medical care effect, the domestic and international market is increasing to the demand of becteriums product.The white spirit of Pleuotus nebrodensis Quel formal name used at school is picked up the ears, and belongs to the Eumycota Basidiomycetes, Pleurotaceae, Pleurotus.Pleurotus nebrodensis sporophore is pure white limpid, and stem is plump, quality is fine and smooth, tender and crisp, mouthfeel is good, nutrition is very abundant, is the integration of drinking and medicinal herbs good merchantable brand.Pleuotus nebrodensis Quel has effects such as long-pending, the desinsection of disappearing, antibechic, anti-inflammatory, control gynecological tumor.According to reports, Pleuotus nebrodensis Quel has the long-pending stagnation resolvation that disappears, clearing heat and detoxicating, effects such as treatment stomach trouble, typhoid fever.Contained fungi polysaccharide can the enhances human body immunologic function.Cholesterol in its unsaturated fatty acid and the blood of human body is combined into cholesterol ester, brings high blood pressure down, prevents arteriosclerotic effect.Contained vitamin D is the choice drug of control children rachitis, osteomalacia and middle-aged and senile osteoporosis.Pleurotus ferulae nutritious abundant, contain 18 seed amino acids in the protein, wherein 8 seed amino acids of needed by human all contain.In addition, also contain multi mineral prime element and vitamin.Pleuotus nebrodensis Quel rich vitamins C, content is up to 26.4 milligrams/100 grams, and general flat mushroom seldom contains vitamin C.Every gram Pleuotus nebrodensis Quel contains in 190 milligrams of amino acid of polysaccharide, and lysine is 569 milligrams/100 grams, is 2.8 times of general flat mushroom; Leucine 790.2 milligrams/100 gram is 2.6 times of general flat mushroom; Isoleucine is 470.1 milligrams/100 grams, is 2.5 times of general flat mushroom; Valine 674.6 milligrams/100 grams are 2.2 times of general flat mushroom, and the amino acid content of other needed by human is also high 1.9～2.7 times than general flat mushroom, and especially glutamic acid and arginine content are high especially.
The culture technique of existing Pleuotus nebrodensis Quel is made cultivate material with cotton seed hulls, because cotton seed hulls has certain gossypol composition, and crude fiber content is high, and the bacterium chaff behind the Pleuotus nebrodensis Quel of gathering can not be made feed, can not make fertilizer again, all goes out of use, and can cause environmental pollution.All the time, vinasse are only as the roughage of ruminant.Cultivate Pleuotus nebrodensis Quel with vinasse, not only can improve the output and the quality of Pleuotus nebrodensis Quel, the bacterium chaff after gathering also can be used as the vinasse animal feeding-stuff containing somatic protein, is used for aquaculture, has avoided the pollution of discarded object to environment.Therefore, the economic benefit of vinasse cultivation Pleuotus nebrodensis Quel technology is obviously high than cottonseed shell cultivation, can increase economic efficiency more than 20%.This Pleuotus nebrodensis Quel culture technique had both found new raw material for the cultivation Pleuotus nebrodensis Quel, for the development and use vinasse break a new path, had obvious social, economic benefit and ecological benefits again.
Summary of the invention
The purpose of this invention is to provide a kind of new Pleuotus nebrodensis Quel cultivation method, it adopts vinasse cultivation Pleuotus nebrodensis Quel, improves the output and the quality of Pleuotus nebrodensis Quel greatly.
In order to realize the object of the invention, the cultivation method of a kind of Pleuotus nebrodensis Quel of the present invention, it all adopts distillers ' grains as cultivate material in each section incubation of Pleuotus nebrodensis Quel original seed, cultivated species and inoculation.
The pH of said distillers ' grains is 7.0～7.5, and available quicklime is regulated, and generally need carry out sterilization treatment.Distillers ' grains is many to be the by product behind the raw material production liquor with the jowar, and crude protein content is 13～17% in the general dry matter, and crude fiber content is 11～17%.
Pleuotus nebrodensis Quel original seed of the present invention carries out female expansion of planting earlier before cultivation numerous, and used medium is a potato culture.In general, its preparation method is: potato 200g, and being cut into small pieces adds the 1000ml running water, boils 30min; Husky cloth filter and remove residue adds glucose 20g, agar 20g; Heating makes it to dissolve, and the moisture that loses when supplying heating is behind the packing test tube; Under 1.02kg pressure, sterilization 50min, the pendulum inclined-plane is subsequent use.Expand numerous cultivation and under 23～25 ℃, carry out, camera bellows was cultivated 20～24 hours, and then cultivated 8～10 days.
The condition of culture of said original seed is: cultivation temperature is 23～29 ℃, and the righttest cultivation temperature is 26 ℃, and incubation time is 33～40 days.
The condition of culture of said cultivated species is: cultivation temperature is 23～29 ℃, and the righttest cultivation temperature is 26 ℃, and incubation time is 33～40 days.
The condition of culture of said inoculation is: cultivation temperature is 24～26 ℃, and incubation time is 50～55 days.
When inoculated and cultured, used vinasse cultivate material can be sterilized, and can play the saving of labor, save time, reduces the effect of cultivating cost.
The present invention adopts vinasse cultivation Pleuotus nebrodensis Quel, not only can improve the output and the quality of Pleuotus nebrodensis Quel, and the bacterium chaff after gathering also can be used as the vinasse animal feeding-stuff containing somatic protein, is used for aquaculture, has avoided the pollution of discarded object to environment, can increase economic efficiency more than 20%; Do cultivate material with fresh vinasse during greenhouse cultivation, need not sterilize, directly operation sequence has been simplified in pack, has reduced production cost, has obvious social, economic benefit and ecological benefits.
Description of drawings
Fig. 1 is the process chart of Pleuotus nebrodensis Quel cultivation method according to the invention.
Embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Embodiment 1
As shown in Figure 1, Pleuotus nebrodensis Quel is cultivated by this technological process, and concrete steps are following:
(1) female test tube of planting expands numerous
From female kind of Pleuotus nebrodensis Quel that Institute of Microorganism, Academia Sinica buys, expand numerous medium that uses and be potato 200g, being cut into small pieces adds the 1000ml running water, boils 30min; Husky cloth filter and remove residue adds glucose 20g, agar 20g; Heating makes it to dissolve, and the moisture that loses when supplying heating is behind the packing test tube; Under 1.02kg pressure, sterilization 50min, the pendulum inclined-plane is subsequent use.
Expanding propagation method is: in common inoculating hood, inoculates, on medium, can a bit inoculate also after the bacterium case is all sterilized and can inoculate by multiple spot, but generally speaking; The inoculation point is placed on the centre of medium slant; Inoculate under back 25 ℃ of conditions, secretly cultivated about 24 hours, lark mushroom mycelia begins to sprout; Whole cultivation stage needs 8 days; Mycelia selects the healthy and strong conduct of pollution-free growing way to produce the sexupara kind after covering with test tube, and assorted bacterium and hypogenetic removing are arranged, general 1 female 30～60 in switchable test tube of planting.
(2) vinasse cultivate material preparation: will transfer pH to 7.0 with quicklime from the pure white vinasse that brewery buys, pack, sterilization.
(3) original seed preparation with cultivate: mother is planted insert the original seed bag that the vinasse cultivate material is housed, put culturing room and cultivate, cultivation temperature is 26 ℃, cultivated 35 days, the original seed of pure white look.
(4) preparation of cultivated species and cultivation: original seed is inserted the cultivated species bag that the vinasse cultivate material is housed, and the inoculum concentration that connects 30 bag cultivating kind bags with every bag of original seed is inoculated, and puts culturing room and cultivates, cultivated 35 days down at 26 ℃, the real cultivated species of hyphal development shape.
(5) greenhouse cultivation: from brewery's roping fresh vinasse, transfer pH to 7.5 with quicklime, unsterilised; Directly pack, every sacked material 0.5kg, the inoculum concentration that connects 30-35 cultivation bag with each cultivation bacterium bag is inoculated; In culturing room, send out bacterium, 26 ℃ of room temperatures can cover with mycelia about 50 days; Change mushroom producing room over to, bacterium rod, in the booth of sterilization in advance with the capacity of 20,000 bacterium rods of every booth; In a row the pile bacterium is excellent, and every yard 8-10 layer can be adopted mushroom in sporophore growth 10-12 days under 15 ℃-20 ℃ natural temperature.
Embodiment 2
As shown in Figure 1, Pleuotus nebrodensis Quel is cultivated by this technological process, and concrete steps are following:
(1) female test tube of planting expands numerous
Employing is expanded the female kind of Pleuotus nebrodensis Quel numerous with embodiment 1 the same method.
(2) vinasse cultivate material preparation: pure white vinasse are transferred pH to 7.5 with quicklime, pack, sterilization.
(3) original seed preparation with cultivate: mother is planted insert the original seed bag that the vinasse cultivate material is housed, put culturing room and cultivate, cultivation temperature is 23 ℃, cultivated 40 days, the original seed of pure white look.
(4) preparation of cultivated species and cultivation: the Pleuotus nebrodensis Quel original seed of buying is inserted the cultivated species bag that the vinasse cultivate material is housed; The inoculum concentration that connects 30 bag cultivating kind bags with every bag of original seed is inoculated; Put culturing room and cultivate, cultivated 33 days down at 29 ℃, the real cultivated species of hyphal development shape.
(5) greenhouse cultivation: from brewery's roping fresh vinasse, transfer pH to 7.2 with quicklime, unsterilised; Directly pack, every sacked material 0.5kg, the inoculum concentration that connects 30-35 cultivation bag with each cultivation bacterium bag is inoculated; In culturing room, send out bacterium, 25 ℃ of room temperatures can cover with mycelia in 50 days; Change mushroom producing room over to, bacterium rod, in the booth of sterilization in advance with the capacity of 20,000 bacterium rods of every booth; In a row the pile bacterium is excellent, and every yard 8-10 layer can be adopted mushroom in sporophore growth 10-12 days under 15 ℃-20 ℃ natural temperature.
Embodiment 3
As shown in Figure 1, Pleuotus nebrodensis Quel is cultivated by this technological process, and concrete steps are following:
(1) female test tube of planting expands numerous
Employing is expanded the female kind of Pleuotus nebrodensis Quel numerous with embodiment 1 the same method.
(2) vinasse cultivate material preparation: pure white vinasse are transferred pH to 7.2 with quicklime, pack, sterilization.
(3) original seed preparation with cultivate: mother is planted insert the original seed bag that the vinasse cultivate material is housed, put culturing room and cultivate, cultivation temperature is 29 ℃, cultivated 33 days, the original seed of pure white look.
(4) preparation of cultivated species and cultivation: original seed is inserted the cultivated species bag that the vinasse cultivate material is housed, and the inoculum concentration that connects 30 bag cultivating kind bags with every bag of original seed is inoculated, and puts culturing room and cultivates, cultivated 40 days down at 23 ℃, the real cultivated species of hyphal development shape.
(5) greenhouse cultivation: from brewery's roping fresh vinasse, transfer pH to 7.0 with quicklime, unsterilised; Directly pack, every sacked material 0.5kg, the inoculum concentration that connects 30-35 cultivation bag with each cultivation bacterium bag is inoculated; In culturing room, send out bacterium, 24 ℃ of room temperatures can cover with mycelia in 55 days; Change mushroom producing room over to, bacterium rod, in the booth of sterilization in advance with the capacity of 20,000 bacterium rods of every booth; In a row the pile bacterium is excellent, and every yard 8-10 layer can be adopted mushroom in sporophore growth 10-12 days under 15 ℃-20 ℃ natural temperature.
Test Example
This Test Example has been carried out the comparative trial with distillers ' grains cultivation Pleuotus nebrodensis Quel and conventional method, cottonseed shell cultivation Pleuotus nebrodensis Quel.
For shortening test period, directly carry out fruiting experiment with cultivated species, the breeding condition that adopts distillers ' grains is with embodiment 1, with cottonseed shell cultivation lark mushroom; Except that cotton seed hulls will be sterilized, other process conditions were with embodiment 1, and the fruiting temperature is at 18 ℃; Observe record squaring period, the young mushroom phase, become mushroom picking time; And weigh and carry out calculation of yield, detect the main quality index of two kinds of cultivate material results Pleuotus nebrodensis Quel, the benefit and the quality discrepancy of the Pleuotus nebrodensis Quel that analysis vinasse cultivate material and conventional cotton seed hulls are gathered.Comparative test result is seen table 1:
Table 1 different material cultivation Pleuotus nebrodensis Quel fruiting relatively
Result of the test shows, cultivates that cell age phase that white spirit plants and growth cycle all lack than the Pleuotus nebrodensis Quel of cottonseed shell cultivation and productive rate is high with distillers ' grains.
Claims (1)
1. the cultivation method of a Pleuotus nebrodensis Quel, it comprises the steps:
(1) female test tube of planting expands numerous
Expand numerous medium that uses and be potato 200g, being cut into small pieces adds the 1000ml running water, boils 30min, husky cloth filter and remove residue; Add glucose 20g, agar 20g, heating makes it to dissolve, the moisture that loses when supplying heating; Behind the packing test tube, under 1.02kg pressure, sterilization 50min, the pendulum inclined-plane is subsequent use;
Expanding propagation method is: in common inoculating hood, inoculate, the bacterium case is all sterilized, and the back is a bit inoculated on medium or multiple spot is inoculated, and the inoculation point is placed on the centre of medium slant; Inoculate under back 25 ℃ of conditions, secretly cultivated 24 hours, the Pleuotus nebrodensis Quel mycelia begins to sprout; Whole cultivation stage needs 8 days; Mycelia selects the healthy and strong conduct of pollution-free growing way to produce the sexupara kind after covering with test tube, and assorted bacterium and hypogenetic removing are arranged, 1 female 30～60 in switching test tube of planting;
(2) vinasse cultivate material preparation: pure white vinasse are transferred pH to 7.0 with quicklime, pack, sterilization;
(3) original seed preparation with cultivate: mother is planted insert the original seed bag that the vinasse cultivate material is housed, put culturing room and cultivate, cultivation temperature is 26 ℃, cultivated 35 days, the original seed of pure white look;
(4) preparation of cultivated species and cultivation: original seed is inserted the cultivated species bag that the vinasse cultivate material is housed, and the inoculum concentration that connects 30 bag cultivating kind bags with every bag of original seed is inoculated, and puts culturing room and cultivates, cultivated 35 days down at 26 ℃, the sturdy cultivated species of hyphal development;
(5) greenhouse cultivation: with fresh vinasse, transfer pH to 7.5 with quicklime, unsterilised, directly pack; Every sacked material 0.5kg, the inoculum concentration that connects 30-35 cultivation bag with each cultivation bacterium bag is inoculated, and in culturing room, sends out bacterium, 26 ℃ of room temperatures; Covered with mycelia in 50 days, and changed mushroom producing room over to, bacterium rod, in the booth of sterilization in advance with the capacity of 20,000 bacterium rods of every booth; In a row pile bacterium rod, every yard 8-10 layer was adopted mushroom in sporophore growth 10-12 days under 15 ℃-20 ℃ natural temperature.
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| CN101946638B (en) * | 2010-09-20 | 2011-12-28 | 福建省曲斗香酒业有限公司 | Method for culturing cordyceps militaris by distilled grain |
| CN102584363B (en) * | 2011-12-01 | 2013-11-27 | 浙江塔牌绍兴酒有限公司 | Method for producing medical mycelium or medical and edible dual-purpose mycelium by using yellow wine lees as liquid medium |
| CN103553775B (en) * | 2013-10-24 | 2015-07-01 | 连云港参宝食用菌有限公司 | Culture material for pleurotus nebrodensis and cultivation method of pleurotus nebrodensis |
| CN105165388B (en) * | 2015-07-14 | 2017-09-12 | 邓树元 | A kind of strain Simple inoculation method |
| CN106576897A (en) * | 2016-11-22 | 2017-04-26 | 曹晓龙 | Method for planting alpine lucid ganoderma |
| CN109156266A (en) * | 2018-08-03 | 2019-01-08 | 西北师范大学 | A kind of method schizophyllum commune cultivation culture medium and cultivate schizophyllum commune using it |
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