CN107353061A - A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing - Google Patents
A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing Download PDFInfo
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- CN107353061A CN107353061A CN201710617922.0A CN201710617922A CN107353061A CN 107353061 A CN107353061 A CN 107353061A CN 201710617922 A CN201710617922 A CN 201710617922A CN 107353061 A CN107353061 A CN 107353061A
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- culture medium
- velutipes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing, concretely comprise the following steps：By peeling potatoes, chopping, add boiling to boil, filter to obtain potato fruit；By dregs of beans with etc. the water of quality mix, digest, enzyme deactivation, filtering, be concentrated to give dregs of beans enzymolysis liquid；Monosodium glutamate waste liquid is concentrated to give monosodium glutamate waste liquid concentrate；Potato fruit, dregs of beans enzymolysis liquid, gourmet powder waste water, agar, potassium dihydrogen phosphate, vitamin B1, magnesium sulfate are measured by formula, is well mixed, sterilizing, produces Liquid Culture Medium in Flammuling velutipes Cart. Sing.Have the beneficial effect that：The preparation method simple possible of culture medium of the present invention, is regenerated resource development and utilization, free from environmental pollution, considerably reduces charges for disposing pollutants use, increases resource utilization, has and prepare the characteristics of convenient, cost is low；Culture medium of the present invention have the advantages that strain is with short production cycle, strain quality is easily controlled, access culture bottle or packed culture medium on bacterium germination speed is fast, fruiting is neat, yield is high.
Description
Technical field
The present invention relates to fungus growing technique field, more particularly, to a kind of preparation side of Liquid Culture Medium in Flammuling velutipes Cart. Sing
Method.
Background technology
Asparagus scientific name hair handle money bacterium, also known as hair handle small fire mushroom, plain mushroom, dried mushroom, intelligence mushroom, hair handle money mushroom, golden mushroom
Deng, in Plant Taxonomy, category Eumycota, Basidiomycetes, Agaricales, Tricholomataceae, money Pseudomonas.Asparagus divides in nature
Cloth is extensive, and there is cultivation on the ground such as China, Japan, Russia, Europe, North America, Australia.Asparagus is free of chlorophyll, no
With photosynthesis, it is impossible to manufacture carbohydrate, can be grown completely in dark surrounds, it is necessary to absorbed from culture medium ready-made
Organic substance, such as degradation product of carbohydrate, protein and fat could grow, and be metatrophy type, be a kind of different
Health thing river.Asparagus is a kind of timber saprophytic bacteria, is easily grown on the withered trunk and stub of the broad leaf trees such as willow, elm, white poplar,
It generated mycelia by spore germination before this in growth, the generally white villiform of mycelia, had tabula and branch, many mycelia gather
Gather together to form mycelium, mycelial growth to certain phase will form fructification, and the major function of fructification is to produce to embrace
Son, for raising up seed.Asparagus is autumn and winter and the edible mushroom of early spring cultivation, so that its cap cunning is tender, handle is crisp, nutritious, taste
U.S. is agreeable to the taste and is world-famous for.Liquid deep layer fermenting culture is to use biofermentation culture device, is sent out by Submerged liquid culturation
The mode of ferment produces edible fungi ball, the original as the seed of edible fungus culturing or as extracts active ingredients separation and deep processing
Material.Edible fungus liquid fermentation can obtain substantial amounts of mycelium, and its nutrition and active component content are than son that solid fermentation obtains
Higher ` villages in entity, and the extracellular products secreted during liquid fermentation also has many active components, therefore Liquid Culture
With the advantage that solid culture is incomparable：It is with short production cycle, nutrient utilization is high, production varietal character is stable and product pollution is small
Deng.In recent years, ripe day by day with liquid fermentation technology, substantial amounts of edible mushroom employs liquid fermentation and culture technology, to obtain
More mycelium and cell free fermentation products are obtained, for physiologically actives such as abstraction function protein, polysaccharide, alkaloid, amino acid
Material.
Prior art such as Authorization Notice No. is the B of CN 104072299 Chinese invention patent, discloses a kind of asparagus liquid
Body bacterium culture medium and preparation method thereof, the technical scheme used for：Contain maize flour 45kg, soybean starch per 250L culture mediums
2.5-3kg, edible oil 200ml, surplus are water；Preparation is carried out in accordance with the following steps：（1）Above formula material is dissolved in into water to carry out
Mix and pour into biological fermentation tank, add water to 250L in a reservoir；（2）122 DEG C -126 DEG C of keeping temperature, pressure 2kg/cm2
Lower sterilizing 60 minutes；（3）Culture base-material is cooled to less than 27 DEG C, accesses Needle mushroom strain, 23 DEG C -25 of regulation cultivation temperature
DEG C, it is passed through purification air and carries out aerobic culture, fermented and cultured 70-100h can be prepared by high-quality flammulina velutipes liquid strains.The culture
Base simple process, but the cycle of the medium culture asparagus is longer, cost is higher.
The content of the invention
It is an object of the invention to provide a kind of preparation method simple possible, cost of material is low, nutritious comprehensive, is beneficial to
Strain absorbs, and nitrogen content is sufficient, carbon nitrogen reasonable mixture ratio, strengthens the sprouting ability of strain, makes growth speed fast, shortens
The preparation method of the Liquid Culture Medium in Flammuling velutipes Cart. Sing in growth cycle.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is：A kind of Liquid Culture Medium in Flammuling velutipes Cart. Sing
Preparation method, including potato fruit prepare, dregs of beans enzymolysis liquid prepare, monosodium glutamate waste liquid concentrate prepare, mixture, specific steps
For：
It is prepared by potato fruit：By peeling potatoes, shred, add potato weight 0.01-0.03% active peptides, with
Solid-liquid ratio is 1:4-5 adds boiling to boil 30-40min, and filtered through gauze obtains potato fruit, standby, the amino acid of above-mentioned active peptides
Sequence HSHCFCCGAAKCYLCRVLHPGKLNCSK, the active peptides can destroy the cell membrane material of potato cell, make thin
After birth produces hole, changes the osmotic pressure of cell membrane, enables cellular content material is quick and complete to discharge, and improves Ma Ling
The leaching rates and active ingredient of potato juice, increase the nutritional ingredient of culture medium；
It is prepared by dregs of beans enzymolysis liquid：By dregs of beans with etc. the water of quality mix, regulation pH value then adds 6000-7000U/g to 8-9
Alkali protease, digest 2-3h at 50-60 DEG C, enzyme deactivation, filtering, be concentrated into 50-60%, produce dregs of beans enzymolysis liquid, the step
Soybean protein can resolve into Soybean Peptide, Soybean Peptide has that absorption rate is fast, energy consumption is low, the absorption between amino acid can be avoided competing
The characteristics of being not easy saturation with carrier is striven, amino acid is supplemented in the form of Soybean Peptide, absorption of the Needle mushroom strain to nitrogen can be improved, be
Needle mushroom strain provides sufficient amino acid and nitrogen source, accelerates absorbing for Needle mushroom strain, improves the growth rate of strain
And quality；
Monosodium glutamate waste liquid concentrate prepares：Monosodium glutamate waste liquid concentration is produced into monosodium glutamate waste liquid concentration to be solid containing the concentrate for being 30-40%
Liquid, standby, asparagus can be used as hypha fermentation by the use of the glucose in concentrate, glutamic acid, urea, ammonium acetate and ammonium sulfate etc.
The carbon source or nitrogen source of growth, normal growth conditions are presented, asparagus is preferable to the treatment effect of gourmet powder waste water, and COD is gone
It is to reduce pollution, raising resource utilization effective way using gourmet powder waste water culture asparagus except rate reaches as high as 82%；
Mixture：Formula is measured into potato fruit, dregs of beans enzymolysis liquid, gourmet powder waste water, agar, potassium dihydrogen phosphate, vitamin B1, sulphur
Sour magnesium, it is well mixed, adjusts pH to 6-7, be put into autoclave, sterilize 55-65min under pressure 0.1-0.15Mpa, i.e.,
Liquid Culture Medium in Flammuling velutipes Cart. Sing is obtained, the culture medium is regenerated resource development and utilization, free from environmental pollution, considerably reduces charges for disposing pollutants use, increases
Add resource utilization, have and prepare the characteristics of convenient, cost is low, it is nutritious, the nutrition of abundance can be provided for Needle mushroom strain
Material, it is to provide growing environment preferably in bacterium, substantially reduces incubation time, be adapted to Industry Promotion.
Preferably, the composition and its parts by weight of culture medium are：Potato fruit 20-30 parts, dregs of beans enzymolysis liquid 15-22
Part, gourmet powder waste water 11-15 parts, agar 0.2-0.3 parts, potassium dihydrogen phosphate 0.02-0.04 parts, vitamin B1 0.001-0.002
Part, magnesium sulfate 0.01-0.02 parts.Above-mentioned culture medium contains abundant dietary fiber, protein, Soybean Peptide and vitamin, for gold
Pin mushroom strains provide sufficient nutriment, can accelerate absorbing for Needle mushroom strain, strengthen the sprouting ability of strain, make
Growth speed is fast, and growing way is strong, and mycelia is sturdy, intensive.
Compared with prior art, the advantage of the invention is that：1）The preparation method simple possible of culture medium of the present invention, is useless
Thing regeneration, it is free from environmental pollution, charges for disposing pollutants use is considerably reduced, increases resource utilization, convenient, cost is low with preparing
The characteristics of；2）Soybean protein is resolved into Soybean Peptide by preparation method of the present invention, and amino acid is supplemented in the form of Soybean Peptide, can be improved
Absorption of the Needle mushroom strain to nitrogen, accelerates absorbing for Needle mushroom strain, improves the growth rate and quality of strain；3）This hair
The formula that bright culture medium uses is reasonable, nutritious, disclosure satisfy that culture propagation nutritional need, can accelerate Needle mushroom strain
Absorb, strengthen the sprouting ability of strain, make that growth speed is fast, and growing way is strong, mycelia is sturdy, intensive, has strain life
Production the cycle it is short, strain quality is easily controlled, access culture bottle or packed culture medium on bacterium germination speed is fast, fruiting is neat, yield is high
The advantages that.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing, including prepared by potato fruit, prepared by dregs of beans enzymolysis liquid, monosodium glutamate waste liquid
Concentrate prepares, mixture, concretely comprises the following steps：
1）It is prepared by potato fruit：By peeling potatoes, shred, the active peptides of potato weight 0.01% are added, with feed liquid
Than for 1:4 plus boiling boiling 40min, obtains potato fruit, standby, the amino acid sequence of above-mentioned active peptides through 6 layers of filtered through gauze
HSHCFCCGAAKCYLCRVLHPGKLNCSK, the active peptides can destroy the cell membrane material of potato cell, make cell membrane
Hole is produced, changes the osmotic pressure of cell membrane, enables cellular content material is quick and complete to discharge, improves potato juice
Leaching rates and active ingredient, increase the nutritional ingredient of culture medium；
2）It is prepared by dregs of beans enzymolysis liquid：By dregs of beans with etc. the water of quality mix, regulation pH value to 8, then add 7000U/g alkalescence
Protease, 2h is digested at 60 DEG C, the enzyme deactivation 12min at 100 DEG C, with 8 layers of filtered through gauze, then high temperature is concentrated into 50%, produces
Dregs of beans enzymolysis liquid, the step can resolve into soybean protein Soybean Peptide, and Soybean Peptide has that absorption rate is fast, energy consumption is low, can avoid
The characteristics of absorption competition and carrier between amino acid are not easy saturation, amino acid is supplemented in the form of Soybean Peptide, acupuncture needle can be improved
Absorption of the mushroom strains to nitrogen, the amino acid and nitrogen source of abundance are provided for Needle mushroom strain, accelerate absorbing for Needle mushroom strain,
Improve the growth rate and quality of strain；
3）Monosodium glutamate waste liquid concentrate prepares：Monosodium glutamate waste liquid concentration is produced into monosodium glutamate waste liquid concentration to be solid containing the concentrate for being 40%
Liquid, standby, asparagus can be used as hypha fermentation by the use of the glucose in concentrate, glutamic acid, urea, ammonium acetate and ammonium sulfate etc.
The carbon source or nitrogen source of growth, normal growth conditions are presented, asparagus is preferable to the treatment effect of gourmet powder waste water, and COD is gone
It is to reduce pollution, raising resource utilization effective way using gourmet powder waste water culture asparagus except rate reaches as high as 82%；
4）Mixture：By formula measure potato fruit, dregs of beans enzymolysis liquid, gourmet powder waste water, agar, potassium dihydrogen phosphate, vitamin B1,
Magnesium sulfate, it is well mixed, adjusts pH to 6, be put into autoclave, sterilize 55min under pressure 0.15Mpa, produces acupuncture needle
Mushroom fluid nutrient medium, the culture medium is regenerated resource development and utilization, free from environmental pollution, considerably reduces charges for disposing pollutants use, increases resource
Utilization rate, have and prepare the characteristics of convenient, cost is low, it is nutritious, the nutriment of abundance can be provided for Needle mushroom strain, be
Growing environment preferably is provided in bacterium, substantially reduces incubation time, is adapted to Industry Promotion.
The composition and its parts by weight of above-mentioned culture medium be：22 parts of potato fruit, 22 parts of dregs of beans enzymolysis liquid, gourmet powder waste water 11
Part, 0.3 part of agar, 0.02 part of potassium dihydrogen phosphate, 0.002 part of vitamin B1,0.01 part of magnesium sulfate.Above-mentioned culture medium contains rich
Rich dietary fiber, protein, Soybean Peptide and vitamin, the nutriment of abundance is provided for Needle mushroom strain, gold can be accelerated
Pin mushroom strains absorb, and strengthen the sprouting ability of strain, make that growth speed is fast, and growing way is strong, and mycelia is sturdy, intensive.
Embodiment 2：
A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing, is concretely comprised the following steps：
1）By peeling potatoes, shred, the active peptides of potato weight 0.02% are added, using solid-liquid ratio as 1:4.5 add boiling
35min is boiled, filtered through gauze obtains potato fruit, standby, the amino acid sequence of above-mentioned active peptides
HSHCFCCGAAKCYLCRVLHPGKLNCSK, the active peptides can destroy the cell membrane material of potato cell, make cell membrane
Hole is produced, changes the osmotic pressure of cell membrane, enables cellular content material is quick and complete to discharge, improves potato juice
Leaching rates and active ingredient, increase the nutritional ingredient of culture medium；
2）By dregs of beans with etc. the water of quality mix, regulation pH value to 8.5,6500U/g alkali protease is then added, at 55 DEG C
Lower enzymolysis 2.5h, enzyme deactivation, filtering, 55% being concentrated into, produces dregs of beans enzymolysis liquid, soybean protein can be resolved into Soybean Peptide by the step,
Soybean Peptide has the characteristics of absorption rate is fast, energy consumption is low, absorption competition between amino acid and carrier can be avoided to be not easy saturation, with
The form supplement amino acid of Soybean Peptide, can improve absorption of the Needle mushroom strain to nitrogen, the amino of abundance is provided for Needle mushroom strain
Acid and nitrogen source, accelerate absorbing for Needle mushroom strain, improve the growth rate and quality of strain；
3）Monosodium glutamate waste liquid concentration is produced into monosodium glutamate waste liquid concentrate, standby, asparagus is available to be solid containing the concentrate for being 35%
The carbon source or nitrogen source thing that glucose, glutamic acid, urea, ammonium acetate and ammonium sulfate in concentrate etc. grow as hypha fermentation
Matter, normal growth conditions are presented, asparagus is preferable to the treatment effect of gourmet powder waste water, and COD clearances reach as high as 82%, profit
It is to reduce pollution, raising resource utilization effective way with gourmet powder waste water culture asparagus；
4）25 parts of potato fruit, 18 parts of dregs of beans enzymolysis liquid, 13 parts of gourmet powder waste water, 0.25 part of agar, di(2-ethylhexyl)phosphate are taken by weight
0.03 part of hydrogen potassium, 0.0015 part of vitamin B1,0.015 part of magnesium sulfate, it is well mixed, adjusts pH to 6.5, be put into autoclaving
In device, sterilize 60min under pressure 0.12Mpa, produces Liquid Culture Medium in Flammuling velutipes Cart. Sing, and the culture medium is regenerated resource development and utilization, no
Environment is polluted, charges for disposing pollutants use is considerably reduced, increases resource utilization, have and prepare the characteristics of convenient, cost is low, nutrition is rich
Richness, the nutriment of abundance can be provided for Needle mushroom strain, be to provide growing environment preferably in bacterium, substantially reduce training
The time is supported, is adapted to Industry Promotion.
Embodiment 3：
A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing：By peeling potatoes, chopping, potato weight 0.03% is added
Active peptides, using solid-liquid ratio as 1:4.3 plus boiling boiling 30min, filters to obtain potato fruit；By dregs of beans with etc. the water of quality mix
Close, regulation pH value to 9, then add 7000U/g alkali protease, 2h is digested at 28 DEG C, enzyme deactivation, filtering, is concentrated into
60%, produce dregs of beans enzymolysis liquid；Monosodium glutamate waste liquid concentration is produced into monosodium glutamate waste liquid concentrate to be solid containing the concentrate for being 32%；By weight
Amount part takes 28 parts of potato fruit, 17 parts of dregs of beans enzymolysis liquid, 14 parts of gourmet powder waste water, 0.22 part of agar, potassium dihydrogen phosphate 0.035
Part, 0.0018 part of vitamin B1,0.014 part of magnesium sulfate, it is well mixed, adjusts pH to 7, be put into autoclave, in pressure
Sterilize 62min under 0.11Mpa, produces Liquid Culture Medium in Flammuling velutipes Cart. Sing.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Lanxi City is difficult to understand and special agricultural science and technology Co., Ltd
<120>A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 27
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Cys Phe Cys Cys Gly Ala Ala Lys Cys Tyr Leu Cys Arg
1 5 10 15
Val Leu His Pro Gly Lys Leu Asn Cys Ser Lys
20 25
Claims (10)
1. a kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing, including prepared by potato fruit, prepared by dregs of beans enzymolysis liquid, monosodium glutamate gives up
Concentrated liquor prepares, mixture, it is characterised in that：The potato fruit preparation process is：By peeling potatoes, chopping, then add
Enter active peptides and water, potato fruit is filtered to obtain after boiling.
A kind of 2. preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing according to claim 1, it is characterised in that：The potato
In juice preparation process the addition of active peptides be potato weight 0.01-0.03%, its amino acid sequence
HSHCFCCGAAKCYLCRVLHPGKLNCSK。
A kind of 3. preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing according to claim 1, it is characterised in that：The potato
Using solid-liquid ratio as 1 in juice preparation process:4-5 adds water, boils 30-40min.
A kind of 4. preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing according to claim 1, it is characterised in that：The dregs of beans enzyme
Solving liquid preparation process is：By dregs of beans with etc. the water of quality mix, regulation pH value to 8-9 adds alkali protease, enzymolysis, enzyme deactivation,
Filtering, is concentrated into 50-60%, produces dregs of beans enzymolysis liquid.
A kind of 5. preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing according to claim 1, it is characterised in that：The dregs of beans enzyme
The addition of solution liquid preparation process neutral and alkali protease is 6000-7000U/g, and hydrolysis temperature is 50-60 DEG C, enzymolysis time 2-
3h。
A kind of 6. preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing according to claim 1, it is characterised in that：The monosodium glutamate gives up
Containing admittedly for monosodium glutamate waste liquid concentrate is 30-40% in concentrated liquor preparation process.
A kind of 7. preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing according to claim 1, it is characterised in that：The mixture step
Suddenly it is：Formula is measured into potato fruit, dregs of beans enzymolysis liquid, gourmet powder waste water, agar, potassium dihydrogen phosphate, vitamin B1, sulfuric acid
Magnesium, it is well mixed, adjusts pH, be put into autoclave and sterilize, produce Liquid Culture Medium in Flammuling velutipes Cart. Sing.
A kind of 8. preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing according to claim 7, it is characterised in that：The mixture step
The composition of culture medium and its parts by weight are in rapid：Potato fruit 20-30 parts, dregs of beans enzymolysis liquid 15-22 parts, gourmet powder waste water 11-
15 parts, agar 0.2-0.3 parts, potassium dihydrogen phosphate 0.02-0.04 parts, vitamin B1 0.001-0.002 parts, magnesium sulfate 0.01-
0.02 part.
A kind of 9. preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing according to claim 7, it is characterised in that：The mixture step
The pH to 6-7 of Liquid Culture Medium in Flammuling velutipes Cart. Sing in rapid.
A kind of 10. preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing according to claim 7, it is characterised in that：The mixture
Sterilization pressure is 0.1-0.15Mpa, time 55-65min in step.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109258395A (en) * | 2018-11-20 | 2019-01-25 | 山东友和菌业有限公司 | A kind of flammulina velutipes liquid strains culture solution and preparation method |
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| CN109258395A (en) * | 2018-11-20 | 2019-01-25 | 山东友和菌业有限公司 | A kind of flammulina velutipes liquid strains culture solution and preparation method |
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