CN109258395A - A kind of flammulina velutipes liquid strains culture solution and preparation method - Google Patents
A kind of flammulina velutipes liquid strains culture solution and preparation method Download PDFInfo
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- CN109258395A CN109258395A CN201811381514.0A CN201811381514A CN109258395A CN 109258395 A CN109258395 A CN 109258395A CN 201811381514 A CN201811381514 A CN 201811381514A CN 109258395 A CN109258395 A CN 109258395A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The present invention provides a kind of flammulina velutipes liquid strains culture solution and preparation methods, it comprises the following components in parts by weight: 2-3 parts of bean cake powder, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, 1.5-3 parts of glucose, 1-2 parts of monosodium glutamate, 0.5-1 parts of tomato extracting solution, 0.7-1.2 parts of apple extracting solution, 0.3-0.5 parts of paclobutrazol powder, 0.2-0.4 parts of drop-proof agent powder, 3-5 parts of chitosan, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of trehalose, 2-6 parts of yeast powder, 1-2 parts of peptone, 1-2 parts of potassium dihydrogen phosphate, 0.1-1.5 parts of graphene oxide.On the one hand flammulina velutipes liquid strains culture solution provided by the invention and preparation method provide sufficient nutrient by changing the constituent and content of fluid nutrient medium for the development of Needle mushroom strain, it is ensured that the quick breeding of Needle mushroom strain；Another side avoids Needle mushroom strain space-pollution during post incoulation, and the lysine and arginine content in needle mushroom after simultaneously effective improving plant significantly improve the mouthfeel of needle mushroom.
Description
Technical field
The present invention relates to edible mushroom culture technique field, in particular to a kind of flammulina velutipes liquid strains culture solution and
Preparation method.
Background technique
Needle mushroom scientific name hair handle money bacterium, also known as hair handle small fire mushroom, plain mushroom, dried mushroom, plain wild rice, freeze bacterium, golden mushroom, intelligence at structure bacterium
Power mushroom etc..Because its stem is elongated, like day lily, therefore claims needle mushroom, belong to Agaricales Tricholomataceae needle gold mushroom category, be a kind of bacterium algae lichens
Class.Needle mushroom has very high medicinal dietary function.
Needle mushroom is both a kind of ticbit and preferable health food, and the domestic and international market of needle mushroom is increasingly wide.
Needle mushroom artificial cultivation technique is simultaneously uncomplicated, as long as can control environmental condition, is easy for obtaining reliable and stable yield.According to survey
Fixed, the content of needle mushroom amino acid is very rich, is higher than general mushroom class, reaches in every 100g fresh mushroom containing total amino acid content
20.9mg, wherein 8 kinds of essential amino acids account for the 42.29~51.17% of total amount, isoleucine and content of glutamic acid highest,
Arginine and lysine content are higher in essential amino acid, and the arginine of high level can prevent and treat hepatitis, stomach and intestine feedback disease
Etc. disease of digestive systems, lysine can promote upgrowth and development of children, enhancing memory improves intelligence.Contain egg in needle mushroom dry product
White matter 8.87%, carbohydrate 60.2%, crude fibre are often edible to prevent and treat canker up to 7.4%.But it is normal using this field
Under the premise of rule culture medium, conventional culture methods are cultivated, the content of above-mentioned nutritional ingredient is difficult to increase again.
Meanwhile needle mushroom has aqueous height, tissue tender and crisp, easily causes to damage in harvesting and transporting procedures, causes to become
Color, it is rotten or rotten the features such as.Needle mushroom post harvest transport major physiological Biochemical changes include Tissue Browning, cell wall protein and
Polysaccharide degradation, fructification aging etc., these variations have seriously affected needle mushroom quality.Shorten the main original of needle mushroom shelf life
Because including enzymatic browning and fungus-caused mildew.Cause three kinds of key enzyme polyphenol oxidase PPO, peroxidase of brown stain
POD, cat catalase are distributed in needle mushroom tissue in compartmentalization: the enzymatic activity of cap is minimum, stem top enzymatic activity slightly
Height, middle part is higher, active lower is most strong.Therefore, in storage, the brown stain of needle mushroom is by stem lower part, gradually to
Vertical spread.In the case where no any fresh-keeping measure, the needle mushroom after cleaning will soon cover with white under preference temperature
Fungal hyphae.In 5~10 DEG C of refrigerated shelf, shelf life is also no more than 3~4 days.In face of this problem, art technology
Personnel are fresh-keeping such as the anti-corrosion with sodium pyrosulfite color protection and potassium sorbate usually using traditional needle mushroom, though there is preferable effect
Fruit, but to human health toxic side effect and carcinogenesis.In addition such as VC is used for color protection and Restrain browning, and effect is poor, unstable
It is fixed.Therefore, how to extend the shelf life of needle mushroom, Restrain browning is always insoluble technical problem.
Summary of the invention
The present invention is directed to overcome the shortcomings of to propose a kind of needle mushroom liquid in existing needle mushroom production and Cultivating techniques
Bacteria culture fluid and preparation method.
On the one hand, it the invention proposes a kind of flammulina velutipes liquid strains culture solution, comprises the following components in parts by weight: dregs of beans
2-3 parts of powder, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, 1.5-3 parts of glucose, 1-2 parts of monosodium glutamate,
0.5-1 parts of tomato extracting solution, 0.7-1.2 parts of apple extracting solution, 0.3-0.5 parts of paclobutrazol powder, 0.2-0.4 parts of drop-proof agent powder, shell
3-5 parts of glycan, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of trehalose, 2-6 parts of yeast powder, 1-2 parts of peptone, potassium dihydrogen phosphate 1-2
Part, 0.1-1.5 parts of graphene oxide, 0.1-0.85 parts of N- butyl-pyridinium hexafluorophosphate, sodium carboxymethylcellulose 0.1-0.5
Part, 0.2-0.8 parts of polyacrylamide, 1.3-9.5 parts of nanometer calcium carbonate, 0.2-0.8 parts of sodium lauryl sulfonate, surplus is pure
Water, Medium's PH Value are adjusted to 5-6.
On the other hand, the present invention also provides a kind of preparation method of flammulina velutipes liquid strains culture solution, include the following steps:
Step a: dregs of beans, silkworm chrysalis, yeast and the lichens powder of each half parts by weight are first taken respectively, and joins it into perseverance
45-60min is persistently dried in warm baking oven in 80-85 DEG C of hot environment, then by after drying dregs of beans, silkworm chrysalis, yeast with
And lichens crushes, to respectively obtain bean cake powder, dried silkworm chrysalis meal, yeast powder and lichens powder spare for sieving；
Step b: mature tomato and apple stripping and slicing are first taken respectively, puts into fruit juice mixer after stripping and slicing and sufficiently blends, then mistake
Filter filter residue, respectively put into Centrifuge Cup in 10-15min is centrifuged in centrifuge, finally take supernatant, obtain tomato extracting solution and
Apple extracting solution is spare；
Step c: the monosodium glutamate of the glucose of parts by weight, the trehalose of parts by weight and parts by weight is first taken to be added to 50 weights respectively
In the water for measuring part, continuous heating obtains aqueous solution to being completely dissolved in 75-85 DEG C of water-bath；Then parts by weight are taken respectively
Paclobutrazol powder and the drop-proof agent powder of parts by weight be added in the aqueous solution, and continue heating water bath until the paclobutrazol powder and
The drop-proof agent powder is dissolved completely in the aqueous solution, and it is spare to obtain nutrient solution；
Step d: the bean cake powders of respective half parts by weight, the dried silkworm chrysalis meal of parts by weight, the yeast powder of parts by weight and again are taken respectively
The lichens powder for measuring part is added in the nutrient solution, adds chitosan, magnesium carbonate, trehalose, yeast powder, peptone, di(2-ethylhexyl)phosphate
Hydrogen potassium, graphene oxide, N- butyl-pyridinium hexafluorophosphate, sodium carboxymethylcellulose, polyacrylamide, nanometer calcium carbonate, the moon
Cinnamic alcohol sodium sulfonate, and 10-15min is vibrated in Vltrasonic device at room temperature, it is again heated to 45-55 DEG C, ultrasonic vibration 5-10min,
10-12 hours are stood, culture medium stoste is obtained；
Step e: taking tomato extracting solution and apple extracting solution to be added in the culture medium stoste respectively, to adjust the training
The Ph for supporting base liquid, the water for continuing thereafter with addition remaining weight part shakes uniformly in Vltrasonic device, needed for obtaining after sterilizing
Culture solution.
In above-mentioned steps d, Vltrasonic device is vibrated according to the relationship of following ultrasonic vibration frequencies and heating temperature,
Wherein, in the first temperature section, in 25-40 DEG C of temperature range:
In formula, f1Indicate the real-time vibration frequency of the first temperature section, T indicates the real time temperature in the first temperature section, T0It indicates
With reference to preset temperature value, thermostat temperature is 30 DEG C, and m indicates the metallic element weight in the nutrient solution being added, and M indicates the battalion being added
The gross mass of solid nutrient ingredient in nutrient solution, c indicate the specific heat of nutrient solution, f0Indicate the default vibration frequency of the first temperature section
Rate is 30kHz；
Wherein, in second temperature section, in 40-45 DEG C of temperature range:
In formula, f2Indicate the real-time vibration frequency of second temperature section, T indicates the real time temperature in second temperature section, T10Table
Show that, with reference to preset temperature value, thermostat temperature is 40 DEG C, m indicates the metallic element weight in the nutrient solution being added, and M expression is added
The gross mass of solid nutrient ingredient in nutrient solution, c indicate the specific heat of nutrient solution, f10Indicate the default vibration frequency of second temperature section
Rate is 55kHz；
Wherein, in third temperature section, in 45-55 DEG C of temperature range:
In formula, f3Indicate the real-time vibration frequency of third temperature section, T indicates the real time temperature in third temperature section, T20Table
Show that, with reference to preset temperature value, temperature is 45 DEG C, and c indicates the specific heat of nutrient solution, f20Indicate the default vibration frequency of the first temperature section,
It is 40kHz；
Wherein, the 4th temperature section, in temperature-fall period, in 25-55 DEG C of temperature range:
In formula, f4Indicate the real-time vibration frequency of the 4th temperature section, T indicates that the real time temperature in the 4th temperature section, c indicate
The specific heat of nutrient solution, m indicate the metallic element weight in the nutrient solution being added, and M indicates the solid nutrient in the nutrient solution being added
The gross mass of ingredient, k indicate the weight of graphene oxide, k0Indicate the parts by weight of chitosan, c indicates the specific heat of nutrient solution, f30
The default vibration frequency for indicating the 4th temperature section is 30kHz.
Further, in above-mentioned steps a, the bean cake powder, the dried silkworm chrysalis meal, the yeast powder and the lichens powder
Granularity be 60-80 mesh.
It further, is refrigerated centrifuge process in the centrifugal process, and the revolving speed of centrifuge is in above-mentioned steps b
3500r/min。
Further, in above-mentioned steps c, the paclobutrazol powder and the drop-proof agent powder are used as inducer to use.
Further, in above-mentioned steps e, the pH value of the culture medium stoste is 6.3-6.7.
Compared with prior art, the beneficial effects of the present invention are flammulina velutipes liquid strains culture solutions provided by the invention
And on the one hand preparation method is provided by changing the constituent and content of fluid nutrient medium for the development of Needle mushroom strain
Sufficient nutrient, it is ensured that the quick breeding of Needle mushroom strain；Another side avoids Needle mushroom strain during post incoulation
Space-pollution, the lysine and arginine content in needle mushroom after simultaneously effective improving plant, significantly improves gold
The mouthfeel of needle mushroom.
It is the oxygen-containing groups such as the epoxy group due to surface of graphene oxide, carboxyl, hydroxyl that graphene oxide, which removes metal ion,
Complex reaction occurs for group's energy and the metal ion of metal ion, especially multivalence.The adsorbents knot such as graphene oxide and chitosan
It closes, the composite construction of formation, there is huge specific surface area, absorption property can be enhanced.Since needle mushroom is in cultivating process, need
Increase certain metal trace element to form necessary aminoacid ingredient, but excessive metallic element, and will affect acupuncture needle
Mushroom is the effect of inhibiting blood lipid to increase, reduce cholesterol, prevent and treat cardiovascular and cerebrovascular disease, therefore, Needle mushroom strain culture of the present invention
Liquid occurs complex reaction with magnesium, zinc, calcium and adsorbs, make tenor appropriate by the graphene oxide of addition Rational Composition
Range.Meanwhile the present invention by using adsorbents such as graphene oxide and chitosans in conjunction with, there is huge specific surface area,
Various nutritional ingredients can be organically adsorbed on surface layer, and form the form of strip, in particular, dregs of beans, silkworm chrysalis, yeast and ground
The sticky shape ingredient such as clothing powder is built up after depending on the surface layer of graphene along the surface layer of graphene, according to strip aligned transfer,
Make needle mushroom during the growth process, strain shape is whole.
In particular, the present invention is when graphene oxide, thick zymasis and minor metallic element to be combined,
The rationally supersonic frequency and vibration frequency of control graphene oxide blending constituent, on the one hand makes graphene oxide and chitosan etc.
It sufficiently combines and reaches optimal efficacy of adsorption；On the other hand, pass through the mixing of Multiple components such as metallic element and thick ingredient
Vibration, is adsorbed in graphene most surface layer for metallic element, nutritional ingredient is accumulated in outer layer, separates the two, and needle mushroom is convenient
Draw nutrient.In the first temperature section, the nutrient solution of room temperature and slightly above room temperature, in order to increase the fusion of graphene oxide with
And adsorption capacity increases vibration frequency, graphene oxide is made to expand specific surface in high frequency environment while increasing temperature
Product；Meanwhile vibration frequency increases with the increase of specific gravity shared by metallic element, increases the adsorption energy to metal coherent element
Power makes metal coherent element more preferably be adsorbed on graphene surface layer.During second stage, as temperature increases, vibration frequency
Rate is slowly increased, and in 45 DEG C of temperature, reaches the highest point of vibration frequency, and in this process, graphene oxide reaches best
Adsorption effect, chitosan sufficiently merges with graphene oxide, by huge specific surface area, in suitable temperature and vibration
Under frequency, various nutritional ingredients are organically adsorbed on surface layer.In the pyroprocess of phase III, vibration frequency is reduced, is made
Various nutritional ingredients are organically adsorbed on surface layer, and excessively high vibration frequency is avoided to generate disorder.It is reduced in the temperature of fourth stage
In the process, by reducing temperature and reducing vibration frequency, various nutritional ingredients are organically adsorbed on surface layer, and formed stable
The nutrient distribution of streaky shape makes needle mushroom during the growth process, ordered arrangement.
In flammulina velutipes liquid strains culture solution provided by the invention and preparation method, given birth to by being added in culture medium prescription
Long regulator paclobutrazol and drop-proof agent, one side paclobutrazol and drop-proof agent are as inducer, in Needle mushroom strain growth course
It induces Needle mushroom strain that nitrogen of absorption etc. is synthesized various amino acid storages in vivo, and then needle mushroom is effectively promoted
Intracorporal content of glutamic acid；Another aspect paclobutrazol and drop-proof agent are as promotor, in Needle mushroom strain growth course effectively
Ground promotes the division of mycelia, while enhancing the resistance of needle mushroom, and then is effectively guaranteed the yield of needle mushroom.
Further, in flammulina velutipes liquid strains culture solution provided by the invention and preparation method, by culture medium prescription
On the one hand middle addition tomato extracting solution and apple extracting solution realize the effectively adjusting to culture medium pH value, on the other hand
Effectively increase the nutritional ingredient in culture medium, it is ensured that the abundance of nutrient needed for the Needle mushroom strain later period develops.
Further, in flammulina velutipes liquid strains culture solution provided by the invention and preparation method, by being carried out to culture medium
Ultrasonication, make each component in culture medium has arrived sufficient mixing, ensure that the nutriment at each position in culture medium
Uniformity, and then the homogeneity that nutriment distributes in later period Needle mushroom strain growth course is effectively ensured, further protect
The synchronism of Needle mushroom strain growth is demonstrate,proved.
Further, in flammulina velutipes liquid strains culture solution provided by the invention and preparation method, nutrient solution is being carried out
During, by accurately controlling the heating temperature of water-bath, ensure that makes often while not destroying this body structure of each component
One component can access maximum dissolution, and then ensure each component in subsequent Needle mushroom strain incubation fully
Play its due effect.
Specific embodiment
Below with reference to specific embodiment, the technical scheme of the present invention will be further described, but claimed range is simultaneously
It is not limited to this.
The embodiment of the present invention proposes the embodiment of the present invention and proposes a kind of flammulina velutipes liquid strains culture solution, including following
The component of parts by weight: 2-3 parts of bean cake powder, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, glucose
1.5-3 parts, 1-2 parts of monosodium glutamate, 0.5-1 parts of tomato extracting solution, 0.7-1.2 parts of apple extracting solution, 0.3-0.5 parts of paclobutrazol powder are prevented
Fall plain powder 0.2-0.4 parts, 3-5 parts of chitosan, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of trehalose, 2-6 parts of yeast powder, peptone
1-2 parts, 1-2 parts of potassium dihydrogen phosphate, 0.1-1.5 parts of graphene oxide, 0.1-0.85 parts of N- butyl-pyridinium hexafluorophosphate, carboxylic first
0.1-0.5 parts of base sodium cellulosate, 0.2-0.8 parts of polyacrylamide, 1.3-9.5 parts of nanometer calcium carbonate, sodium lauryl sulfonate 0.2-
0.8 part, surplus is pure water, and Medium's PH Value is adjusted to 5-6.
On the other hand, the embodiment of the present invention also provides a kind of preparation method of flammulina velutipes liquid strains culture solution, including such as
Lower step:
Step a: dregs of beans, silkworm chrysalis, yeast and the lichens powder of each half parts by weight are first taken respectively, and joins it into perseverance
45-60min is persistently dried in warm baking oven in 80-85 DEG C of hot environment, then by after drying dregs of beans, silkworm chrysalis, yeast with
And lichens crushes, to respectively obtain bean cake powder, dried silkworm chrysalis meal, yeast powder and lichens powder spare for sieving；
Step b: mature tomato and apple stripping and slicing are first taken respectively, puts into fruit juice mixer after stripping and slicing and sufficiently blends, then mistake
Filter filter residue, respectively put into Centrifuge Cup in 10-15min is centrifuged in centrifuge, finally take supernatant, obtain tomato extracting solution and
Apple extracting solution is spare；
Step c: the monosodium glutamate of the glucose of parts by weight, the trehalose of parts by weight and parts by weight is first taken to be added to 50 weights respectively
In the water for measuring part, continuous heating obtains aqueous solution to being completely dissolved in 75-85 DEG C of water-bath；Then parts by weight are taken respectively
Paclobutrazol powder and the drop-proof agent powder of parts by weight be added in the aqueous solution, and continue heating water bath until the paclobutrazol powder and
The drop-proof agent powder is dissolved completely in the aqueous solution, and it is spare to obtain nutrient solution；
Step d: the bean cake powders of respective half parts by weight, the dried silkworm chrysalis meal of parts by weight, the yeast powder of parts by weight and again are taken respectively
The lichens powder for measuring part is added in the nutrient solution, adds chitosan, magnesium carbonate, trehalose, yeast powder, peptone, di(2-ethylhexyl)phosphate
Hydrogen potassium, graphene oxide, N- butyl-pyridinium hexafluorophosphate, sodium carboxymethylcellulose, polyacrylamide, nanometer calcium carbonate, the moon
Cinnamic alcohol sodium sulfonate, and 10-15min is vibrated in Vltrasonic device at room temperature, it is again heated to 45-55 DEG C, ultrasonic vibration 5-10min,
10-12 hours are stood, culture medium stoste is obtained；
Step e: taking tomato extracting solution and apple extracting solution to be added in the culture medium stoste respectively, to adjust the training
The Ph for supporting base liquid, the water for continuing thereafter with addition remaining weight part shakes uniformly in Vltrasonic device, needed for obtaining after sterilizing
Culture solution.
In above-mentioned steps d, Vltrasonic device is vibrated according to the relationship of following ultrasonic vibration frequencies and heating temperature,
Wherein, in the first temperature section, in 25-40 DEG C of temperature range:
In formula, f1Indicate the real-time vibration frequency of the first temperature section, T indicates the real time temperature in the first temperature section, T0It indicates
With reference to preset temperature value, thermostat temperature is 30 DEG C, and m indicates the metallic element weight in the nutrient solution being added, and M indicates the battalion being added
The gross mass of solid nutrient ingredient in nutrient solution, c indicate the specific heat of nutrient solution, f0Indicate the default vibration frequency of the first temperature section
Rate is 30kHz；
Wherein, in second temperature section, in 40-45 DEG C of temperature range:
In formula, f2Indicate the real-time vibration frequency of second temperature section, T indicates the real time temperature in second temperature section, T10Table
Show that, with reference to preset temperature value, thermostat temperature is 40 DEG C, m indicates the metallic element weight in the nutrient solution being added, and M expression is added
The gross mass of solid nutrient ingredient in nutrient solution, c indicate the specific heat of nutrient solution, f10Indicate the default vibration frequency of second temperature section
Rate is 55kHz；
Wherein, in third temperature section, in 45-55 DEG C of temperature range:
In formula, f3Indicate the real-time vibration frequency of third temperature section, T indicates the real time temperature in third temperature section, T20Table
Show that, with reference to preset temperature value, temperature is 45 DEG C, and c indicates the specific heat of nutrient solution, f20Indicate the default vibration frequency of the first temperature section,
It is 40kHz；
Wherein, the 4th temperature section, in temperature-fall period, in 25-55 DEG C of temperature range:
In formula, f4Indicate the real-time vibration frequency of the 4th temperature section, T indicates that the real time temperature in the 4th temperature section, c indicate
The specific heat of nutrient solution, m indicate the metallic element weight in the nutrient solution being added, and M indicates the solid nutrient in the nutrient solution being added
The gross mass of ingredient, k indicate the weight of graphene oxide, k0Indicate the parts by weight of chitosan, c indicates the specific heat of nutrient solution, f30
The default vibration frequency for indicating the 4th temperature section is 30kHz.
Wherein, in the preparation method of above-mentioned flammulina velutipes liquid strains culture solution, in above-mentioned steps a, the bean cake powder, institute
The granularity for stating dried silkworm chrysalis meal, the yeast powder and the lichens powder is 60-80 mesh.
It is cold in the centrifugal process in above-mentioned steps b in the preparation method of above-mentioned flammulina velutipes liquid strains culture solution
Freeze centrifugal process, and the revolving speed of centrifuge is 3500r/min.
In the preparation method of above-mentioned flammulina velutipes liquid strains culture solution, in above-mentioned steps c, the paclobutrazol powder and described
Drop-proof agent powder is used as inducer to use.
In the preparation method of above-mentioned flammulina velutipes liquid strains culture solution, in above-mentioned steps e, the PH of the culture medium stoste
Value is 6.3-6.7.
Embodiment one
The present embodiment improve needle mushroom content of glutamic acid liquid spawn culture medium by following parts by weight component preparation and
At: 3 parts of bean cake powder, 3 parts of dried silkworm chrysalis meal, 0.5 part of yeast powder, 0.5 part of lichens powder, 3 parts of glucose, 3 parts of trehalose, monosodium glutamate 2 part, kind
1 part of eggplant extracting solution, 1.2 parts of apple extracting solution, 0.5 part of paclobutrazol powder and 0.4 part of drop-proof agent powder, 3 parts of chitosan, magnesium carbonate
0.5 part, 20 parts of trehalose, 2 parts of yeast powder, 1 part of peptone, 1 part of potassium dihydrogen phosphate, graphene oxide 0.1, N- butyl-pyridinium six
Fluorophosphate 0.1,0.1 part of sodium carboxymethylcellulose, 0.2 part of polyacrylamide, 1.3 parts of nanometer calcium carbonate, sodium lauryl sulfonate
0.2 part, surplus is pure water, and Medium's PH Value is adjusted to 5-6.
Embodiment two
The present embodiment improve needle mushroom content of glutamic acid liquid spawn culture medium by following parts by weight component preparation and
At: 2-3 parts of bean cake powder, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, 1.5-3 parts of glucose, seaweed
1-3 parts sugared, 1-2 parts of monosodium glutamate, 0.5-1 parts of tomato extracting solution, 0.7-1.2 parts of apple extracting solution, 0.3-0.5 parts of paclobutrazol powder and
0.2-0.4 parts of drop-proof agent powder, surplus is water, 5 parts of chitosan, 2.5 parts of magnesium carbonate, 25 parts of trehalose, 6 parts of yeast powder, peptone 2
Part, 2 parts of potassium dihydrogen phosphate, 1.5 parts of graphene oxide, 0.85 part of N- butyl-pyridinium hexafluorophosphate, sodium carboxymethylcellulose 0.5
Part, 0.8 part of polyacrylamide, 9.5 parts of nanometer calcium carbonate, 0.8 part of sodium lauryl sulfonate, surplus is pure water, Medium's PH Value
It is adjusted to 5-6.
Embodiment three
The present embodiment improve needle mushroom content of glutamic acid liquid spawn culture medium by following parts by weight component preparation and
At: 3 parts of bean cake powder, 2 parts of dried silkworm chrysalis meal, 0.5 part of yeast powder, 0.1 part of lichens powder, 3 parts of glucose, 1 part of trehalose, monosodium glutamate 2 part, kind
0.5 part of eggplant extracting solution, 1.2 parts of apple extracting solution, 0.3 part of paclobutrazol powder and 0.4 part of drop-proof agent powder, surplus is water, chitosan 4
Part, 2 parts of magnesium carbonate, 22 parts of trehalose, 4 parts of yeast powder, 1.5 parts of peptone, 1.5 parts of potassium dihydrogen phosphate, 1 part of graphene oxide,
0.5 part of N- butyl-pyridinium hexafluorophosphate, 0.3 part of sodium carboxymethylcellulose, 0.5 part of polyacrylamide, 5 parts of nanometer calcium carbonate,
0.5 part of sodium lauryl sulfonate, surplus is pure water, and Medium's PH Value is adjusted to 5-6.
Example IV
The present embodiment improve needle mushroom content of glutamic acid liquid spawn culture medium by following parts by weight component preparation and
At: 2 parts of bean cake powder, 3 parts of dried silkworm chrysalis meal, 0.3 part of yeast powder, 0.5 part of lichens powder, 1.5 parts of glucose, 3 parts of trehalose, 1 part of monosodium glutamate,
1 part of tomato extracting solution, 0.7 part of apple extracting solution, 0.5 part of paclobutrazol powder and 0.2 part of drop-proof agent powder, surplus is water, chitosan
3.5 parts, 1.5 parts of magnesium carbonate, 22 parts of trehalose, 4 parts of yeast powder, 1.2 parts of peptone, 0.8 part of potassium dihydrogen phosphate, graphene oxide
1.2 parts, 0.4 part of N- butyl-pyridinium hexafluorophosphate, 0.3 part of sodium carboxymethylcellulose, 0.6 part of polyacrylamide, nano-calcium carbonate
4.5 parts of calcium, 0.4 part of sodium lauryl sulfonate, surplus is pure water, and Medium's PH Value is adjusted to 5-6.
Embodiment five
2 parts of bean cake powder, 2 parts of dried silkworm chrysalis meal, 0.3 part of yeast powder, 0.1 part of lichens powder, 1.5 parts of glucose, 1 part of trehalose, taste
Smart 1 part, 0.5 part of tomato extracting solution, 0.7 part of apple extracting solution, 0.3 part of paclobutrazol powder and 0.2 part of drop-proof agent powder, surplus are
Water, 4.5 parts of chitosan, 2.2 parts of magnesium carbonate, 25 parts of trehalose, 6 parts of yeast powder, 2 parts of peptone, 2 parts of potassium dihydrogen phosphate, oxidation
1.5 parts of graphene, 0.2 part of N- butyl-pyridinium hexafluorophosphate, 0.1 part of sodium carboxymethylcellulose, 0.2 part of polyacrylamide, receive
1.3 parts of calcium carbonate of rice, 0.2 part of sodium lauryl sulfonate, surplus is pure water, and Medium's PH Value is adjusted to 5-6.
In the various embodiments described above, the preparation process of the liquid spawn culture medium of needle mushroom content of glutamic acid is improved are as follows:
Step a: dregs of beans, silkworm chrysalis, yeast and the lichens powder of each half parts by weight are first taken respectively, and joins it into perseverance
45-60min is persistently dried in warm baking oven in 80-85 DEG C of hot environment, then by after drying dregs of beans, silkworm chrysalis, yeast with
And lichens crushes, to respectively obtain bean cake powder, dried silkworm chrysalis meal, yeast powder and lichens powder spare for sieving；
Step b: mature tomato and apple stripping and slicing are first taken respectively, puts into fruit juice mixer after stripping and slicing and sufficiently blends, then mistake
Filter filter residue, respectively put into Centrifuge Cup in 10-15min is centrifuged in centrifuge, finally take supernatant, obtain tomato extracting solution and
Apple extracting solution is spare；
Step c: the monosodium glutamate of the glucose of parts by weight, the trehalose of parts by weight and parts by weight is first taken to be added to 50 weights respectively
In the water for measuring part, continuous heating obtains aqueous solution to being completely dissolved in 75-85 DEG C of water-bath；Then parts by weight are taken respectively
Paclobutrazol powder and the drop-proof agent powder of parts by weight be added in the aqueous solution, and continue heating water bath until the paclobutrazol powder and
The drop-proof agent powder is dissolved completely in the aqueous solution, and it is spare to obtain nutrient solution；
Step d: the bean cake powders of respective half parts by weight, the dried silkworm chrysalis meal of parts by weight, the yeast powder of parts by weight and again are taken respectively
The lichens powder for measuring part is added in the nutrient solution, adds chitosan, magnesium carbonate, trehalose, yeast powder, peptone, di(2-ethylhexyl)phosphate
Hydrogen potassium, graphene oxide, N- butyl-pyridinium hexafluorophosphate, sodium carboxymethylcellulose, polyacrylamide, nanometer calcium carbonate, the moon
Cinnamic alcohol sodium sulfonate, and 10-15min is vibrated in Vltrasonic device at room temperature, it is again heated to 45-55 DEG C, ultrasonic vibration 5-10min,
10-12 hours are stood, culture medium stoste is obtained；
Step e: taking tomato extracting solution and apple extracting solution to be added in the culture medium stoste respectively, to adjust the training
Base liquid pH is supported, the water for continuing thereafter with addition remaining weight part shakes uniformly in Vltrasonic device, and required training is obtained after sterilizing
Nutrient solution.
After Needle mushroom strain culture in above each formula, takes out 300 circles at random respectively and measure, measurement result mean value
It is as follows:
Obviously, flammulina velutipes liquid strains culture solution provided by the invention and preparation method, by changing fluid nutrient medium
On the one hand constituent and content provide sufficient nutrient for the development of Needle mushroom strain, effectively ensure golden mushroom
The quick breeding of kind, greatly improves the yield of needle mushroom；Another side avoids Needle mushroom strain during post incoulation
Space-pollution, the content of glutamic acid in needle mushroom after simultaneously effective improving plant significantly improves needle mushroom
Mouthfeel.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (6)
1. a kind of flammulina velutipes liquid strains culture solution, which is characterized in that comprise the following components in parts by weight: 2-3 parts of bean cake powder, silkworm
2-3 parts of pupa powder, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, 1.5-3 parts of glucose, 1-2 parts of monosodium glutamate, tomato extracting solution
0.5-1 parts, 0.7-1.2 parts of apple extracting solution, 0.3-0.5 parts of paclobutrazol powder, 0.2-0.4 parts of drop-proof agent powder, 3-5 parts of chitosan,
0.5-2.5 parts of magnesium carbonate, 20-25 parts of trehalose, 2-6 parts of yeast powder, 1-2 parts of peptone, aoxidize stone by 1-2 parts of potassium dihydrogen phosphate
Black alkene 0.1-1.5 parts, 0.1-0.85 parts of N- butyl-pyridinium hexafluorophosphate, 0.1-0.5 parts of sodium carboxymethylcellulose, polyacrylamide
0.2-0.8 parts of amine, 1.3-9.5 parts of nanometer calcium carbonate, 0.2-0.8 parts of sodium lauryl sulfonate, surplus is pure water, Medium's PH Value
It is adjusted to 5-6.
2. a kind of preparation method of flammulina velutipes liquid strains culture solution, which comprises the steps of:
Step a: first taking dregs of beans, silkworm chrysalis, yeast and the lichens powder of each half parts by weight respectively, and joins it into constant temperature baking
45-60min is persistently dried in case in 80-85 DEG C of hot environment, then by dregs of beans, silkworm chrysalis, yeast and the ground after drying
It is spare that clothing crushes, sieving respectively obtains bean cake powder, dried silkworm chrysalis meal, yeast powder and lichens powder；
Step b: mature tomato and apple stripping and slicing are first taken respectively, puts into fruit juice mixer after stripping and slicing and sufficiently blends, then filter out
Filter residue is put into Centrifuge Cup be centrifuged 10-15min in centrifuge respectively, finally takes supernatant, obtain tomato extracting solution and apple
Extracting solution is spare；
Step c: the monosodium glutamate of the glucose of parts by weight, the trehalose of parts by weight and parts by weight is first taken to be added to 50 parts by weight respectively
Water in, continuous heating obtains aqueous solution to being completely dissolved in 75-85 DEG C of water-bath；Then the more of parts by weight are taken respectively
The drop-proof agent powder for imitating azoles powder and parts by weight is added in the aqueous solution, and continues heating water bath until the paclobutrazol powder and described
Drop-proof agent powder is dissolved completely in the aqueous solution, and it is spare to obtain nutrient solution；
Step d: bean cake powder, the dried silkworm chrysalis meal of parts by weight, the yeast powder of parts by weight and the parts by weight of respective half parts by weight are taken respectively
Lichens powder be added in the nutrient solution, add chitosan, magnesium carbonate, trehalose, yeast powder, peptone, potassium dihydrogen phosphate,
Graphene oxide, N- butyl-pyridinium hexafluorophosphate, sodium carboxymethylcellulose, polyacrylamide, nanometer calcium carbonate, laruyl alcohol sulphur
Sour sodium, and 10-15min is vibrated in Vltrasonic device at room temperature, it is again heated to 45-55 DEG C, ultrasonic vibration 5-10min, stands 10-
12 hours, obtain culture medium stoste；
Step e: taking tomato extracting solution and apple extracting solution to be added in the culture medium stoste respectively, to adjust the culture medium
Stoste pH, the water for continuing thereafter with addition remaining weight part shake uniformly in Vltrasonic device, and required culture solution is obtained after sterilizing；
In above-mentioned steps d, Vltrasonic device is vibrated according to the relationship of following ultrasonic vibration frequencies and heating temperature,
Wherein, in the first temperature section, in 25-40 DEG C of temperature range:
In formula, f1Indicate the real-time vibration frequency of the first temperature section, T indicates the real time temperature in the first temperature section, T0Indicate reference
Preset temperature value, thermostat temperature are 30 DEG C, and m indicates the metallic element weight in the nutrient solution being added, and M indicates the nutrient solution being added
In solid nutrient ingredient gross mass, c indicate nutrient solution specific heat, f0Indicate the default vibration frequency of the first temperature section,
For 30kHz；
Wherein, in second temperature section, in 40-45 DEG C of temperature range:
In formula, f2Indicate the real-time vibration frequency of second temperature section, T indicates the real time temperature in second temperature section, T10Indicate ginseng
Preset temperature value is examined, thermostat temperature is 40 DEG C, and m indicates the metallic element weight in the nutrient solution being added, and M indicates the nutrition being added
The gross mass of solid nutrient ingredient in liquid, c indicate the specific heat of nutrient solution, f10Indicate the default vibration frequency of second temperature section,
It is 55kHz；
Wherein, in third temperature section, in 45-55 DEG C of temperature range:
In formula, f3Indicate the real-time vibration frequency of third temperature section, T indicates the real time temperature in third temperature section, T20Indicate ginseng
Preset temperature value is examined, temperature is 45 DEG C, and c indicates the specific heat of nutrient solution, f20The default vibration frequency for indicating the first temperature section is
40kHz；
Wherein, the 4th temperature section, in temperature-fall period, in 25-55 DEG C of temperature range:
In formula, f4Indicate the real-time vibration frequency of the 4th temperature section, T indicates that the real time temperature in the 4th temperature section, c indicate nutrition
The specific heat of liquid, m indicate the metallic element weight in the nutrient solution being added, and M indicates the solid nutrient ingredient in the nutrient solution being added
Gross mass, k indicate graphene oxide weight, k0Indicate the parts by weight of chitosan, c indicates the specific heat of nutrient solution, f30It indicates
The default vibration frequency of 4th temperature section is 30kHz.
3. the preparation method of flammulina velutipes liquid strains culture solution according to claim 2, which is characterized in that in above-mentioned steps
In a, the bean cake powder, the dried silkworm chrysalis meal, the yeast powder and the lichens powder granularity be 60-80 mesh.
4. the preparation method of flammulina velutipes liquid strains culture solution according to claim 2, which is characterized in that in above-mentioned steps
It is refrigerated centrifuge process in the centrifugal process, and the revolving speed of centrifuge is 3500r/min in b.
5. the preparation method of flammulina velutipes liquid strains culture solution according to claim 2, which is characterized in that in above-mentioned steps
In c, the paclobutrazol powder and the drop-proof agent powder are used as inducer to use.
6. the preparation method of flammulina velutipes liquid strains culture solution according to claim 2, which is characterized in that in above-mentioned steps
In e, the pH value of the culture medium stoste is 6.3-6.7.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439649A (en) * | 2018-11-20 | 2019-03-08 | 山东友和菌业有限公司 | A kind of selection of yellow needle mushroom breeding |
| CN109699395A (en) * | 2019-03-14 | 2019-05-03 | 山东友和菌业有限公司 | A kind of bottle of cultivation needle mushroom breeding method |
| CN111925250A (en) * | 2020-08-17 | 2020-11-13 | 贵州同辉食用菌发展有限公司 | Mushroom liquid strain culture solution |
| CN113170699A (en) * | 2020-08-18 | 2021-07-27 | 山西大同大学 | Graphene synergistic morchella culture medium and preparation method and application thereof |
| CN114190224A (en) * | 2021-10-28 | 2022-03-18 | 连云港丰泽源生物科技有限公司 | Method for improving content of lysine in needle mushroom |
| CN114885956A (en) * | 2022-06-06 | 2022-08-12 | 中国农业科学院蔬菜花卉研究所 | Bare-grown control method for tomato plug seedlings |
| CN116686630A (en) * | 2023-07-04 | 2023-09-05 | 上海永大菌业有限公司 | Method for cultivating flammulina velutipes by using crop straws |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106700136A (en) * | 2016-11-18 | 2017-05-24 | 武汉理工大学 | Graphene/chitosan composite material and preparation method thereof |
| CN107353061A (en) * | 2017-07-26 | 2017-11-17 | 兰溪市奥而特农业科技有限公司 | A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing |
| CN107586723A (en) * | 2017-07-26 | 2018-01-16 | 浦江县欧立生物技术有限公司 | The cultural method of flammulina velutipes liquid strains |
| CN107750892A (en) * | 2017-10-23 | 2018-03-06 | 柯江波 | Graphene is modified indoor hydroponic plant nutrient solution and preparation method thereof |
| CN108517303A (en) * | 2018-03-12 | 2018-09-11 | 淮海工学院 | A kind of preparation method of flammulina velutipes liquid strains |
| CN108739057A (en) * | 2018-05-09 | 2018-11-06 | 山东省科创食用菌产业技术研究院 | A kind of method that can extend edible fungus solid spawn storage time |
| CN108812066A (en) * | 2018-07-06 | 2018-11-16 | 山东省科创食用菌产业技术研究院 | A kind of liquid spawn culture medium formula and technique improving Pleurotus eryngii content of glutamic acid |
-
2018
- 2018-11-20 CN CN201811381514.0A patent/CN109258395A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106700136A (en) * | 2016-11-18 | 2017-05-24 | 武汉理工大学 | Graphene/chitosan composite material and preparation method thereof |
| CN107353061A (en) * | 2017-07-26 | 2017-11-17 | 兰溪市奥而特农业科技有限公司 | A kind of preparation method of Liquid Culture Medium in Flammuling velutipes Cart. Sing |
| CN107586723A (en) * | 2017-07-26 | 2018-01-16 | 浦江县欧立生物技术有限公司 | The cultural method of flammulina velutipes liquid strains |
| CN107750892A (en) * | 2017-10-23 | 2018-03-06 | 柯江波 | Graphene is modified indoor hydroponic plant nutrient solution and preparation method thereof |
| CN108517303A (en) * | 2018-03-12 | 2018-09-11 | 淮海工学院 | A kind of preparation method of flammulina velutipes liquid strains |
| CN108739057A (en) * | 2018-05-09 | 2018-11-06 | 山东省科创食用菌产业技术研究院 | A kind of method that can extend edible fungus solid spawn storage time |
| CN108812066A (en) * | 2018-07-06 | 2018-11-16 | 山东省科创食用菌产业技术研究院 | A kind of liquid spawn culture medium formula and technique improving Pleurotus eryngii content of glutamic acid |
Non-Patent Citations (1)
| Title |
|---|
| 王广阔等: "不同超声频率与温度对纳米ZnO 颗粒分散性能的影响", 《纺织科技进展》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439649A (en) * | 2018-11-20 | 2019-03-08 | 山东友和菌业有限公司 | A kind of selection of yellow needle mushroom breeding |
| CN109699395A (en) * | 2019-03-14 | 2019-05-03 | 山东友和菌业有限公司 | A kind of bottle of cultivation needle mushroom breeding method |
| CN111925250A (en) * | 2020-08-17 | 2020-11-13 | 贵州同辉食用菌发展有限公司 | Mushroom liquid strain culture solution |
| CN113170699A (en) * | 2020-08-18 | 2021-07-27 | 山西大同大学 | Graphene synergistic morchella culture medium and preparation method and application thereof |
| CN114190224A (en) * | 2021-10-28 | 2022-03-18 | 连云港丰泽源生物科技有限公司 | Method for improving content of lysine in needle mushroom |
| CN114190224B (en) * | 2021-10-28 | 2023-12-22 | 连云港丰泽源生物科技有限公司 | Method for improving lysine content of flammulina velutipes |
| CN114885956A (en) * | 2022-06-06 | 2022-08-12 | 中国农业科学院蔬菜花卉研究所 | Bare-grown control method for tomato plug seedlings |
| CN114885956B (en) * | 2022-06-06 | 2023-06-09 | 中国农业科学院蔬菜花卉研究所 | Free growth control method for tomato plug seedlings |
| CN116686630A (en) * | 2023-07-04 | 2023-09-05 | 上海永大菌业有限公司 | Method for cultivating flammulina velutipes by using crop straws |
| CN116686630B (en) * | 2023-07-04 | 2024-01-30 | 上海永大菌业有限公司 | Method for cultivating flammulina velutipes by using crop straws |
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