CN108812066A - A kind of liquid spawn culture medium formula and technique improving Pleurotus eryngii content of glutamic acid - Google Patents
A kind of liquid spawn culture medium formula and technique improving Pleurotus eryngii content of glutamic acid Download PDFInfo
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Abstract
The present invention provides a kind of liquid spawn culture medium formulas and technique for improving Pleurotus eryngii content of glutamic acid.Wherein, which comprises the following components in parts by weight：2-3 parts of bean cake powder, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, 1.5-3 parts of glucose, 1-3 parts of trehalose, 1-2 parts of monosodium glutamate, 0.5-1 parts of tomato extracting solution, 0.7-1.2 parts of apple extracting solution, 0.3-0.5 parts of paclobutrazol powder and 0.2-0.4 parts of drop-proof agent powder, surplus is water, and the sum of parts by weight of above-mentioned each component are 100.In the present invention, by changing the constituent and content of fluid nutrient medium, sufficient nutrient on the one hand is provided for the development of pleurotus eryngii quel strains, it is ensured that the quick breeding of pleurotus eryngii quel strains；Another side avoids pleurotus eryngii quel strains space-pollution during post incoulation, and the content of glutamic acid in Pleurotus eryngii after simultaneously effective improving plant significantly improves the mouthfeel of Pleurotus eryngii.
Description
Technical field
The present invention relates to edible mushroom culture technique fields, in particular to a kind of raising Pleurotus eryngii content of glutamic acid
Liquid spawn culture medium formula and technique.
Background technique
Pleurotus eryngii (Pleurotus eryngii Quel) also known as perverse celery are picked up the ears, and Eumycota, Basidiomycetes, umbrella are under the jurisdiction of
Zoopagales, Pleurotaceae, Pleurotus.Pleurotus eryngii bacterial context is plump, and quality is tender and crisp, especially stem dense structure, solid, milky white, can be complete
Portion is edible, and stem cunning more crisp than cap, tasty and refreshing, referred to as " oyster mushroom king ", " dried scallop mushroom ", has pleasant almond flavor and such as
The mouthfeel of abalone is suitble to fresh-keeping, processing, firmly gets liking for people, as market is to Pleurotus eryngii demand also day and all increasings, people
To the mouthfeel of Pleurotus eryngii, higher requirements are also raised.However, solid spawn incubation time used by current each manufacturer
Period is long, and space infection is easy to produce during solid vaccination, seriously affects the mouthfeel of later period Pleurotus eryngii.
Summary of the invention
The present invention is directed to overcome the shortcomings of to propose a kind of raising Pleurotus eryngii in existing Pleurotus eryngii production and Cultivating techniques
The liquid spawn culture medium formula and technique of content of glutamic acid.
On the one hand, the invention proposes a kind of liquid spawn culture medium formulas for improving Pleurotus eryngii content of glutamic acid, including
The component of following parts by weight：
2-3 parts of bean cake powder, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, glucose 1.5-3
Part, 1-3 parts of trehalose, 1-2 parts of monosodium glutamate, 0.5-1 parts of tomato extracting solution, 0.7-1.2 parts of apple extracting solution, paclobutrazol powder 0.3-
0.5 part and 0.2-0.4 parts of drop-proof agent powder, surplus is water, and the sum of parts by weight of above-mentioned each component are 100.
On the other hand, the invention proposes a kind of liquid spawn culture medium technique for improving Pleurotus eryngii content of glutamic acid, packets
Include following steps：
Step 1：Dregs of beans, silkworm chrysalis, yeast and lichens are first taken respectively, and are joined it into constant temperature oven in 80-85
DEG C hot environment in persistently dry 45-60min, then dregs of beans, silkworm chrysalis, yeast and the lichens after drying are crushed, sieving
It is spare to respectively obtain bean cake powder, dried silkworm chrysalis meal, yeast powder and lichens powder；
Step 2：Mature tomato and apple stripping and slicing are first taken respectively, are put into fruit juice mixer after stripping and slicing and are sufficiently blended, then mistake
Filter residue is filtered, puts into Centrifuge Cup be centrifuged 10-15min in centrifuge respectively, takes supernatant finally to get tomato extracting solution is arrived
It is spare with apple extracting solution；
Step 3：The taste of the glucose of 1.5-3 parts by weight, the trehalose of 1-3 parts by weight and 1-2 parts by weight is first taken respectively
Finishing enters into the water of 50 parts by weight, and continuous heating obtains aqueous solution to being completely dissolved in 75-85 DEG C of water-bath；Then
The drop-proof agent powder of the paclobutrazol powder and 0.2-0.4 parts by weight that take 0.3-0.5 parts by weight respectively is added in the aqueous solution, and continues
Heating water bath is dissolved completely in the aqueous solution up to the paclobutrazol powder and the drop-proof agent powder, and it is spare to obtain nutrient solution；
Step 4：Take respectively the bean cake powder of 2-3 parts by weight, the dried silkworm chrysalis meal of 2-3 parts by weight, 0.3-0.5 parts by weight yeast powder
It is added in the nutrient solution with the lichens powder of 0.1-0.5 parts by weight, and shakes 10-15min in Vltrasonic device, obtain culture medium
Stoste；
Step 5：The apple extracting solution of the tomato extracting solution and 0.7-1.2 parts by weight that take 0.5-1 parts by weight respectively is added to
In the culture medium stoste, to adjust the PH of the culture medium stoste, the water that remaining weight part is added is continued thereafter in ultrasound dress
It sets middle concussion uniformly, required culture solution is obtained after sterilizing.
Further, in the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in above-mentioned steps 1
In, the bean cake powder, the dried silkworm chrysalis meal, the yeast powder and the lichens powder granularity be 60-80 mesh.
Further, in the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in above-mentioned steps 2
In, it is refrigerated centrifuge process in the centrifugal process, and the revolving speed of centrifuge is 3500r/min.
Further, in the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in above-mentioned steps 3
In, the paclobutrazol powder and the drop-proof agent powder are used as inducer to use.
Further, in the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in above-mentioned steps 4
In, the oscillation frequency of the Vltrasonic device is 30-45kHz.
Further, in the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in above-mentioned steps 5
In, the pH value of the culture medium stoste is 6.3-6.7.
Further, in above-mentioned steps 3, three heating sheets is set while being heated, also, heating sheet is respectively set
The first temperature sensor, second temperature is respectively set on upper, preceding, right three side walls of water-bath, also, on corresponding side wall
Sensor, third temperature sensor, the detection temperature Φ of the first temperature sensor, second temperature sensor, third temperature sensing
It is respectively Φ 2, Φ 3 that device, which detects temperature,；Further include a control unit, controls each temperature information respectively, and pass to each temperature
The temperature of sensor is acquired and compares, and described control unit sets difference degree of balance threshold value as M；
Control unit is according to following difference degrees of balance for calculating the first temperature sensor and second temperature sensor：
In formula, M21Indicate the difference degree of balance of the first temperature sensor and second temperature sensor, Φ indicates the first temperature
The real-time detection value of sensor, Φ 2 indicate the real-time detection value of second temperature sensor, and Φ 3 indicates third temperature sensor
Real-time detection value, T indicate mean square deviation operation, and I indicates integral operation.
Wherein I indicates any integral operation based on quadratic function, and above-mentioned formula is the ratio information for obtaining integral, following
Two formula are identical, are such as based on function y=ax2, it is a in (a, b) in x value<B is any number；
Control unit is according to following initial differences for calculating the first temperature sensor and third temperature sensor：
In formula, M31Indicate the difference degree of balance of the first temperature sensor and third temperature sensor, Φ indicates the first temperature
The real-time detection value of sensor, Φ 2 indicate the real-time detection value of second temperature sensor, and Φ 3 indicates third temperature sensor
Real-time detection value, T indicate mean square deviation operation, and I indicates integral operation；
Control unit is according to following initial differences for calculating second temperature sensor and third temperature sensor：
In formula, M23Indicate the difference degree of balance of second temperature sensor and third temperature sensor, Φ indicates the first temperature
The real-time detection value of sensor, Φ 2 indicate the real-time detection value of second temperature sensor, and Φ 3 indicates third temperature sensor
Real-time detection value, T indicate mean square deviation operation, and I indicates integral operation；
Described control unit sets difference degree of balance threshold value as M, calculates resulting M by above-mentioned formula21、M31、M23, point
It is not compared with difference degree of balance threshold value M, if M21、M31、M23Respectively less than M, the then at this point, temperature difference of each sensor detection
Value control is in a certain range.
Compared with prior art, the beneficial effects of the present invention are raising Pleurotus eryngii content of glutamic acid provided by the invention
Liquid spawn culture medium formula and technique, by change fluid nutrient medium constituent and content, be on the one hand Pleurotus eryngii
The development of strain provides sufficient nutrient, it is ensured that the quick breeding of pleurotus eryngii quel strains；Another side avoids pleurotus eryngii quel strains
Space-pollution during post incoulation, the content of glutamic acid in Pleurotus eryngii after simultaneously effective improving plant, greatly
Ground improves the mouthfeel of Pleurotus eryngii.
Especially, it is provided by the invention improve Pleurotus eryngii content of glutamic acid liquid spawn culture medium formula and technique in,
By the way that growth regulator paclobutrazol and drop-proof agent are added in culture medium prescription, one side paclobutrazol and drop-proof agent are as induction
Agent induces pleurotus eryngii quel strains that nitrogen of absorption etc. is synthesized various amino acid in pleurotus eryngii quel strains growth course and is stored in body
It is interior, and then the intracorporal content of glutamic acid of Pleurotus eryngii is effectively promoted；Another aspect paclobutrazol and drop-proof agent as promotor,
The division of mycelia is effectively promoted in pleurotus eryngii quel strains growth course, while enhancing the resistance of Pleurotus eryngii, and then effectively
Ground ensure that the yield of Pleurotus eryngii.
Further, it is provided by the invention improve Pleurotus eryngii content of glutamic acid liquid spawn culture medium formula and technique in,
By the way that tomato extracting solution and apple extracting solution are added in culture medium prescription, on the one hand realize to the effective of culture medium pH value
Ground is adjusted, and on the other hand effectively increases the nutritional ingredient in culture medium, it is ensured that is supported needed for the development of pleurotus eryngii quel strains later period
The abundance divided.
Further, it is provided by the invention improve Pleurotus eryngii content of glutamic acid liquid spawn culture medium formula and technique in,
By carrying out ultrasonication to culture medium, make each component in culture medium has arrived sufficient mixing, ensure that in culture medium
The nutriment uniformity at each position, and then effectively ensure what nutriment in later period pleurotus eryngii quel strains growth course distributed
Homogeneity further ensures the synchronism of pleurotus eryngii quel strains growth.
Further, it is provided by the invention improve Pleurotus eryngii content of glutamic acid liquid spawn culture medium formula and technique in,
During carrying out nutrient solution, by accurately controlling the heating temperature of water-bath, it ensure that and do not destroying each component sheet
Make each component that can access maximum dissolution while body structure, and then ensures each component in subsequent pleurotus eryngii quel strains
Its due effect is fully played in incubation.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the water-bath of the embodiment of the present invention.
Specific embodiment
Below with reference to specific embodiment, the technical scheme of the present invention will be further described, but claimed range is simultaneously
It is not limited to this.
The embodiment of the present invention propose it is a kind of improve Pleurotus eryngii content of glutamic acid liquid spawn culture medium formula, including with
The component of lower parts by weight：
2-3 parts of bean cake powder, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, glucose 1.5-3
Part, 1-3 parts of trehalose, 1-2 parts of monosodium glutamate, 0.5-1 parts of tomato extracting solution, 0.7-1.2 parts of apple extracting solution, paclobutrazol powder 0.3-
0.5 part and 0.2-0.4 parts of drop-proof agent powder, surplus is water, and the sum of parts by weight of above-mentioned each component are 100.
The embodiment of the invention provides a kind of liquid spawn culture medium techniques for improving Pleurotus eryngii content of glutamic acid, including such as
Lower step：
Step 1：Dregs of beans, silkworm chrysalis, yeast and lichens are first taken respectively, and are joined it into constant temperature oven in 80-85
DEG C hot environment in persistently dry 45-60min, then dregs of beans, silkworm chrysalis, yeast and the lichens after drying are crushed, sieving
It is spare to respectively obtain bean cake powder, dried silkworm chrysalis meal, yeast powder and lichens powder；
Step 2：Mature tomato and apple stripping and slicing are first taken respectively, are put into fruit juice mixer after stripping and slicing and are sufficiently blended, then mistake
Filter residue is filtered, puts into Centrifuge Cup be centrifuged 10-15min in centrifuge respectively, takes supernatant finally to get tomato extracting solution is arrived
It is spare with apple extracting solution；
Step 3：The taste of the glucose of 1.5-3 parts by weight, the trehalose of 1-3 parts by weight and 1-2 parts by weight is first taken respectively
Finishing enters into the water of 50 parts by weight, and continuous heating obtains aqueous solution to being completely dissolved in 75-85 DEG C of water-bath；Then
The drop-proof agent powder of the paclobutrazol powder and 0.2-0.4 parts by weight that take 0.3-0.5 parts by weight respectively is added in the aqueous solution, and continues
Heating water bath is dissolved completely in the aqueous solution up to the paclobutrazol powder and the drop-proof agent powder, and it is spare to obtain nutrient solution；
Step 4：Take respectively the bean cake powder of 2-3 parts by weight, the dried silkworm chrysalis meal of 2-3 parts by weight, 0.3-0.5 parts by weight yeast powder
It is added in the nutrient solution with the lichens powder of 0.1-0.5 parts by weight, and shakes 10-15min in Vltrasonic device, obtain culture medium
Stoste；
Step 5：The apple extracting solution of the tomato extracting solution and 0.7-1.2 parts by weight that take 0.5-1 parts by weight respectively is added to
In the culture medium stoste, to adjust the PH of the culture medium stoste, the water that remaining weight part is added is continued thereafter in ultrasound dress
It sets middle concussion uniformly, required culture solution is obtained after sterilizing.
In the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in above-mentioned steps 1, the dregs of beans
Powder, the dried silkworm chrysalis meal, the yeast powder and the lichens powder granularity be 60-80 mesh.
In the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in step 2 above, the centrifugation
It is in the process refrigerated centrifuge process, and the revolving speed of centrifuge is 3500r/min.
In the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in above-mentioned steps 3, the multiple-effect
Azoles powder and the drop-proof agent powder are used as inducer to use.
In the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in above-mentioned steps 4, the ultrasound
The oscillation frequency of device is 30-45kHz.
In the liquid spawn culture medium technique of above-mentioned raising Pleurotus eryngii content of glutamic acid, in above-mentioned steps 5, the culture
The pH value of base liquid is 6.3-6.7.
The present embodiment is heated simultaneously by three heating sheets of setting, also, heating sheet is separately positioned on water-bath
On upper, preceding, right three side walls, also, the first temperature sensor 21, second temperature sensor is respectively set on corresponding side wall
22, third temperature sensor 23, the detection temperature Φ of the first temperature sensor 21, second temperature sensor 22, third temperature pass
It is respectively Φ that sensor 23, which detects temperature,2、Φ3.Further include a control unit, controls each temperature information respectively, and to each temperature
The temperature of sensor is acquired and compares, and described control unit sets difference degree of balance threshold value as M；
Control unit is according to following difference degrees of balance for calculating the first temperature sensor and second temperature sensor：
In formula, M21Indicate the difference degree of balance of the first temperature sensor and second temperature sensor, Φ indicates the first temperature
The real-time detection value of sensor, Φ2Indicate the real-time detection value of second temperature sensor, Φ3Indicate the reality of third temperature sensor
When detected value, T indicate mean square deviation operation, I indicate integral operation.
Wherein I indicates any integral operation based on quadratic function, and above-mentioned formula is the ratio information for obtaining integral, following
Two formula are identical, are such as based on function y=ax2, it is a in (a, b) in x value<B is any number.
The rudimentary algorithm of above-mentioned mean operation is：By obtaining the positional value of all sampled points within a certain period of time,
Integral operation and mean square deviation operation are carried out to each value in some period, ratio is then taken, show that is compared is averaged
Value.
Control unit is according to following initial differences for calculating the first temperature sensor and third temperature sensor：
In formula, M31Indicate the difference degree of balance of the first temperature sensor and third temperature sensor, Φ indicates the first temperature
The real-time detection value of sensor, Φ2Indicate the real-time detection value of second temperature sensor, Φ3Indicate the reality of third temperature sensor
When detected value, T indicate mean square deviation operation, I indicate integral operation.
Control unit is according to following initial differences for calculating second temperature sensor and third temperature sensor：
In formula, M23Indicate the difference degree of balance of second temperature sensor and third temperature sensor, Φ indicates the first temperature
The real-time detection value of sensor, Φ2Indicate the real-time detection value of second temperature sensor, Φ3Indicate the reality of third temperature sensor
When detected value, T indicate mean square deviation operation, I indicate integral operation.
Described control unit sets difference degree of balance threshold value as M, calculates resulting M by above-mentioned formula21、M31、M23, point
It is not compared with difference degree of balance threshold value M, if M21、M31、M23Respectively less than M, the then at this point, temperature difference of each sensor detection
Value can guarantee that cultivation package has the consistency of temperature in certain control range.
If above-mentioned formula calculates resulting M21、M31、M23, it is compared respectively with difference degree of balance threshold value M, there are M21、
M31、M23Any difference is greater than M, then control unit controls the heating of heating sheet corresponding to corresponding temperature sensor or cooling, directly
To meeting M21、M31、M23Respectively less than M.
The present embodiment influences greatly content of glutamic acid due to being applied with monosodium glutamate, glucose, trehalose, dissolution degree,
Also, therefore the excessively high failure or damage that will also result in paclobutrazol powder and drop-proof agent powder of temperature answers strict control heating temperature,
Ensure that makes each component that can access maximum dissolution while not destroying this body structure of each component, and then ensures
Each component fully plays its due effect in subsequent pleurotus eryngii quel strains incubation.In the present embodiment, control unit
For a circuit board, it is arranged on water-bath outer wall of wall or is arranged in electric cabinet.
Embodiment one
The present embodiment improve Pleurotus eryngii content of glutamic acid liquid spawn culture medium by following parts by weight component preparation and
At：3 parts of bean cake powder, 3 parts of dried silkworm chrysalis meal, 0.5 part of yeast powder, 0.5 part of lichens powder, 3 parts of glucose, 3 parts of trehalose, monosodium glutamate 2 part, kind
1 part of eggplant extracting solution, 1.2 parts of apple extracting solution, 0.5 part of paclobutrazol powder and 0.4 part of drop-proof agent powder, surplus is water, above-mentioned each group
The sum of parts by weight divided are 100.
Embodiment two
The present embodiment improve Pleurotus eryngii content of glutamic acid liquid spawn culture medium by following parts by weight component preparation and
At：2-3 parts of bean cake powder, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, 1.5-3 parts of glucose, seaweed
1-3 parts sugared, 1-2 parts of monosodium glutamate, 0.5-1 parts of tomato extracting solution, 0.7-1.2 parts of apple extracting solution, 0.3-0.5 parts of paclobutrazol powder and
0.2-0.4 parts of drop-proof agent powder, surplus is water, and the sum of parts by weight of above-mentioned each component are 100.
Embodiment three
The present embodiment improve Pleurotus eryngii content of glutamic acid liquid spawn culture medium by following parts by weight component preparation and
At：3 parts of bean cake powder, 2 parts of dried silkworm chrysalis meal, 0.5 part of yeast powder, 0.1 part of lichens powder, 3 parts of glucose, 1 part of trehalose, monosodium glutamate 2 part, kind
0.5 part of eggplant extracting solution, 1.2 parts of apple extracting solution, 0.3 part of paclobutrazol powder and 0.4 part of drop-proof agent powder, surplus is water, above-mentioned each
The sum of parts by weight of component are 100.
Example IV
The present embodiment improve Pleurotus eryngii content of glutamic acid liquid spawn culture medium by following parts by weight component preparation and
At：2 parts of bean cake powder, 3 parts of dried silkworm chrysalis meal, 0.3 part of yeast powder, 0.5 part of lichens powder, 1.5 parts of glucose, 3 parts of trehalose, 1 part of monosodium glutamate,
1 part of tomato extracting solution, 0.7 part of apple extracting solution, 0.5 part of paclobutrazol powder and 0.2 part of drop-proof agent powder, surplus is water, above-mentioned each
The sum of parts by weight of component are 100.
Embodiment five
2 parts of bean cake powder, 2 parts of dried silkworm chrysalis meal, 0.3 part of yeast powder, 0.1 part of lichens powder, 1.5 parts of glucose, 1 part of trehalose, taste
Smart 1 part, 0.5 part of tomato extracting solution, 0.7 part of apple extracting solution, 0.3 part of paclobutrazol powder and 0.2 part of drop-proof agent powder, surplus are
Water, the sum of parts by weight of above-mentioned each component are 100.
In the various embodiments described above, the preparation process for improving the liquid spawn culture medium of Pleurotus eryngii content of glutamic acid is：
Step 1：Dregs of beans, silkworm chrysalis, yeast and lichens are first taken respectively, and are joined it into constant temperature oven in 80-85
DEG C hot environment in persistently dry 45-60min, then dregs of beans, silkworm chrysalis, yeast and the lichens after drying are crushed, sieving
It is spare that 60-80 mesh respectively obtains bean cake powder, dried silkworm chrysalis meal, yeast powder and lichens powder；
Step 2：Mature tomato and apple stripping and slicing are first taken respectively, are put into fruit juice mixer after stripping and slicing and are sufficiently blended, then mistake
Filter filter residue, respectively put into Centrifuge Cup in centrifuge with the revolving speed refrigerated centrifuge 10-15min of 3500r/min, finally take
Clear liquid is to get spare to tomato extracting solution and apple extracting solution；
Step 3：The taste of the glucose of 1.5-3 parts by weight, the trehalose of 1-3 parts by weight and 1-2 parts by weight is first taken respectively
Finishing enters into the water of 50 parts by weight, and continuous heating obtains aqueous solution to being completely dissolved in 75-85 DEG C of water-bath；Then
The drop-proof agent powder of the paclobutrazol powder and 0.2-0.4 parts by weight that take 0.3-0.5 parts by weight respectively is added in the aqueous solution, and continues
Heating water bath is dissolved completely in the aqueous solution up to the paclobutrazol powder and the drop-proof agent powder, and it is spare to obtain nutrient solution；
Step 4：Take respectively the bean cake powder of 2-3 parts by weight, the dried silkworm chrysalis meal of 2-3 parts by weight, 0.3-0.5 parts by weight yeast powder
It is added in the nutrient solution with the lichens powder of 0.1-0.5 parts by weight, and shakes 10-15min in the Vltrasonic device of 30-45KHz,
Obtain culture medium stoste；
Step 5：The apple extracting solution of the tomato extracting solution and 0.7-1.2 parts by weight that take 0.5-1 parts by weight respectively is added to
In the culture medium stoste, to adjust the pH value of the culture medium stoste, make the control of its pH value between 6.3-6.7, then
The water for continuously adding remaining weight part shakes uniformly in the Vltrasonic device of 30-45KHz, and required culture solution is obtained after sterilizing.
It should be noted that the preparation process each component of the liquid spawn culture medium of above-mentioned raising Pleurotus eryngii content of glutamic acid
Parts by weight summation be necessary for 100.
After pleurotus eryngii quel strains culture in above each formula, random taking-up 300 is measured respectively, measurement result mean value
It is as follows：
Obviously, the liquid spawn culture medium formula and technique provided by the invention for improving Pleurotus eryngii content of glutamic acid, passes through
The constituent and content for changing fluid nutrient medium, on the one hand provide sufficient nutrient for the development of pleurotus eryngii quel strains, effectively
Ground ensures the quick breeding of pleurotus eryngii quel strains, greatly improves the yield of Pleurotus eryngii；Another side avoids pleurotus eryngii quel strains
Space-pollution during post incoulation, the content of glutamic acid in Pleurotus eryngii after simultaneously effective improving plant, greatly
Ground improves the mouthfeel of Pleurotus eryngii.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (8)
1. a kind of liquid spawn culture medium formula for improving Pleurotus eryngii content of glutamic acid, which is characterized in that including following parts by weight
Component：2-3 parts of bean cake powder, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.1-0.5 parts of lichens powder, 1.5-3 parts of glucose,
1-3 parts of trehalose, 1-2 parts of monosodium glutamate, 0.5-1 parts of tomato extracting solution, 0.7-1.2 parts of apple extracting solution, 0.3-0.5 parts of paclobutrazol powder
And 0.2-0.4 parts of drop-proof agent powder, surplus is water, and the sum of parts by weight of above-mentioned each component are 100.
2. a kind of liquid spawn culture medium technique for improving Pleurotus eryngii content of glutamic acid, which is characterized in that include the following steps：
Step 1：Dregs of beans, silkworm chrysalis, yeast and lichens are first taken respectively, and are joined it into constant temperature oven at 80-85 DEG C
45-60min is persistently dried in hot environment, is then crushed dregs of beans, silkworm chrysalis, yeast and the lichens after drying, sieving is distinguished
It is spare to obtain bean cake powder, dried silkworm chrysalis meal, yeast powder and lichens powder；
Step 2：Mature tomato and apple stripping and slicing are first taken respectively, are put into fruit juice mixer after stripping and slicing and are sufficiently blended, then filter out
Filter residue is put into Centrifuge Cup be centrifuged 10-15min in centrifuge respectively, takes supernatant finally to get tomato extracting solution and apple is arrived
Fruit extracting solution is spare；
Step 3：The monosodium glutamate of the glucose of 1.5-3 parts by weight, the trehalose of 1-3 parts by weight and 1-2 parts by weight is first taken to add respectively
Enter into the water of 50 parts by weight, continuous heating obtains aqueous solution to being completely dissolved in 75-85 DEG C of water-bath；Then distinguish
The drop-proof agent powder of the paclobutrazol powder and 0.2-0.4 parts by weight that take 0.3-0.5 parts by weight is added in the aqueous solution, and continues water-bath
Heating is dissolved completely in the aqueous solution up to the paclobutrazol powder and the drop-proof agent powder, and it is spare to obtain nutrient solution；
Step 4：Take respectively the bean cake powder of 2-3 parts by weight, the dried silkworm chrysalis meal of 2-3 parts by weight, 0.3-0.5 parts by weight yeast powder and
The lichens powder of 0.1-0.5 parts by weight is added in the nutrient solution, and 10-15min is shaken in Vltrasonic device, obtains culture base
Liquid；
Step 5：The apple extracting solution of the tomato extracting solution and 0.7-1.2 parts by weight that take 0.5-1 parts by weight respectively is added to described
In culture medium stoste, to adjust the PH of the culture medium stoste, the water that remaining weight part is added is continued thereafter in Vltrasonic device
Concussion uniformly, obtains required culture solution after sterilizing.
3. the liquid spawn culture medium technique according to claim 2 for improving Pleurotus eryngii content of glutamic acid, which is characterized in that
In above-mentioned steps 1, the bean cake powder, the dried silkworm chrysalis meal, the yeast powder and the lichens powder granularity be 60-80
Mesh.
4. the liquid spawn culture medium technique according to claim 2 for improving Pleurotus eryngii content of glutamic acid, which is characterized in that
It in step 2 above, is refrigerated centrifuge process in the centrifugal process, and the revolving speed of centrifuge is 3500r/min.
5. the liquid spawn culture medium technique according to claim 2 for improving Pleurotus eryngii content of glutamic acid, which is characterized in that
In above-mentioned steps 3, the paclobutrazol powder and the drop-proof agent powder are used as inducer to use.
6. the liquid spawn culture medium technique according to claim 2 for improving Pleurotus eryngii content of glutamic acid, which is characterized in that
In above-mentioned steps 4, the oscillation frequency of the Vltrasonic device is 30-45kHz.
7. the liquid spawn culture medium technique according to claim 2 for improving Pleurotus eryngii content of glutamic acid, which is characterized in that
In above-mentioned steps 5, the pH value of the culture medium stoste is 6.3-6.7.
8. the liquid spawn culture medium technique according to claim 2 for improving Pleurotus eryngii content of glutamic acid, which is characterized in that
In above-mentioned steps 3, three heating sheets are set while being heated, also, heating sheet be separately positioned on water-bath it is upper, preceding,
On right three side walls, also, the first temperature sensor, second temperature sensor, third temperature is respectively set on corresponding side wall
Sensor, the detection temperature Φ of the first temperature sensor, second temperature sensor, third temperature sensor detection temperature are respectively
Φ2,Φ3；Further include a control unit, controls each temperature information respectively, and adopt to the temperature of each temperature sensor
Collect and compare, described control unit sets difference degree of balance threshold value as M；
Control unit is according to following difference degrees of balance for calculating the first temperature sensor and second temperature sensor：
In formula, M21Indicate the difference degree of balance of the first temperature sensor and second temperature sensor, Φ indicates the first temperature sensing
The real-time detection value of device, Φ 2 indicate the real-time detection value of second temperature sensor, and Φ 3 indicates the real-time of third temperature sensor
Detected value, T indicate mean square deviation operation, and I indicates integral operation.
Wherein I indicates any integral operation based on quadratic function, and above-mentioned formula is the ratio information for obtaining integral, and following two is public
Formula is identical, is such as based on function y=ax2, it is a in (a, b) in x value<B is any number；
Control unit is according to following initial differences for calculating the first temperature sensor and third temperature sensor：
In formula, M31Indicate the difference degree of balance of the first temperature sensor and third temperature sensor, Φ indicates the first temperature sensing
The real-time detection value of device, Φ 2 indicate the real-time detection value of second temperature sensor, and Φ 3 indicates the real-time of third temperature sensor
Detected value, T indicate mean square deviation operation, and I indicates integral operation；
Control unit is according to following initial differences for calculating second temperature sensor and third temperature sensor：
In formula, M23Indicate the difference degree of balance of second temperature sensor and third temperature sensor, Φ indicates the first temperature sensing
The real-time detection value of device, Φ 2 indicate the real-time detection value of second temperature sensor, and Φ 3 indicates the real-time of third temperature sensor
Detected value, T indicate mean square deviation operation, and I indicates integral operation；
Described control unit sets difference degree of balance threshold value as M, calculates resulting M by above-mentioned formula21、M31、M23, respectively with
Difference degree of balance threshold value M is compared, if M21、M31、M23Respectively less than M, then at this point, the temperature gap control of each sensor detection
System is in a certain range.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197388A (en) * | 2018-11-22 | 2019-01-15 | 山东友和菌业有限公司 | A kind of culture medium and preparation method of yellow needle mushroom |
| CN109258395A (en) * | 2018-11-20 | 2019-01-25 | 山东友和菌业有限公司 | A kind of flammulina velutipes liquid strains culture solution and preparation method |
| CN109464892A (en) * | 2018-12-28 | 2019-03-15 | 启明星宇节能科技股份有限公司 | Denitration automatic control system |
| CN109618820A (en) * | 2018-12-10 | 2019-04-16 | 山东友和菌业有限公司 | A kind of the control equipment and its control technique of golden mushroom plantation kind culture environment |
| CN109845574A (en) * | 2018-12-10 | 2019-06-07 | 山东友和菌业有限公司 | Mould detection method in a kind of acupuncture needle mushroom culture |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391034A (en) * | 2011-03-24 | 2012-03-28 | 上海雪榕生物科技股份有限公司 | Formula and preparation method of Pleurotus eryngii liquid strain culture medium |
| CN103992177A (en) * | 2014-05-20 | 2014-08-20 | 广西壮族自治区农业科学院植物保护研究所 | High-yield cultivation method for pleurotus eryngii and culture medium therefor |
| CN106187448A (en) * | 2016-06-30 | 2016-12-07 | 广西壮族自治区农业科学院微生物研究所 | Pleurotus edible fungus fast breeding nutritional solution and the preprocess method of cultigen thereof |
| CN106888799A (en) * | 2017-01-20 | 2017-06-27 | 陆川县新英食用菌专业合作社 | A kind of cultural method of black fungus rich in selenium |
| CN107285929A (en) * | 2017-08-17 | 2017-10-24 | 安徽农耕年华农业发展有限公司 | A kind of pleurotus eryngii efficient culture medium and preparation method thereof |
| CN107568893A (en) * | 2017-10-09 | 2018-01-12 | 杨辰润 | A kind of Anti-shortsightedness pupil desk |
| CN107827640A (en) * | 2017-11-13 | 2018-03-23 | 常州琨瑶纺织品有限公司 | A kind of tobacco-planting soil conditioner and preparation method thereof |
| CN107937329A (en) * | 2017-11-21 | 2018-04-20 | 石家庄学院 | A kind of method for improving liquid spawn vigor |
| CN108235517A (en) * | 2018-01-25 | 2018-06-29 | 深圳民爆光电技术有限公司 | A kind of lighting driving device for protecting eyesight |
-
2018
- 2018-07-06 CN CN201810736054.2A patent/CN108812066A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391034A (en) * | 2011-03-24 | 2012-03-28 | 上海雪榕生物科技股份有限公司 | Formula and preparation method of Pleurotus eryngii liquid strain culture medium |
| CN103992177A (en) * | 2014-05-20 | 2014-08-20 | 广西壮族自治区农业科学院植物保护研究所 | High-yield cultivation method for pleurotus eryngii and culture medium therefor |
| CN106187448A (en) * | 2016-06-30 | 2016-12-07 | 广西壮族自治区农业科学院微生物研究所 | Pleurotus edible fungus fast breeding nutritional solution and the preprocess method of cultigen thereof |
| CN106888799A (en) * | 2017-01-20 | 2017-06-27 | 陆川县新英食用菌专业合作社 | A kind of cultural method of black fungus rich in selenium |
| CN107285929A (en) * | 2017-08-17 | 2017-10-24 | 安徽农耕年华农业发展有限公司 | A kind of pleurotus eryngii efficient culture medium and preparation method thereof |
| CN107568893A (en) * | 2017-10-09 | 2018-01-12 | 杨辰润 | A kind of Anti-shortsightedness pupil desk |
| CN107827640A (en) * | 2017-11-13 | 2018-03-23 | 常州琨瑶纺织品有限公司 | A kind of tobacco-planting soil conditioner and preparation method thereof |
| CN107937329A (en) * | 2017-11-21 | 2018-04-20 | 石家庄学院 | A kind of method for improving liquid spawn vigor |
| CN108235517A (en) * | 2018-01-25 | 2018-06-29 | 深圳民爆光电技术有限公司 | A kind of lighting driving device for protecting eyesight |
Non-Patent Citations (3)
| Title |
|---|
| 安旭 等著: "《城市园林植物后期养护管理学-园林养护单位工作手册》", 31 May 2013, 浙江大学出版社 * |
| 曾小兰 主编: "《食品微生物及其检验技术》", 31 August 2010, 中国轻工业出版社 * |
| 李春龙 等主编: "《植物组织培养》", 31 July 2016, 西南交通大学出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258395A (en) * | 2018-11-20 | 2019-01-25 | 山东友和菌业有限公司 | A kind of flammulina velutipes liquid strains culture solution and preparation method |
| CN109197388A (en) * | 2018-11-22 | 2019-01-15 | 山东友和菌业有限公司 | A kind of culture medium and preparation method of yellow needle mushroom |
| CN109618820A (en) * | 2018-12-10 | 2019-04-16 | 山东友和菌业有限公司 | A kind of the control equipment and its control technique of golden mushroom plantation kind culture environment |
| CN109845574A (en) * | 2018-12-10 | 2019-06-07 | 山东友和菌业有限公司 | Mould detection method in a kind of acupuncture needle mushroom culture |
| CN109464892A (en) * | 2018-12-28 | 2019-03-15 | 启明星宇节能科技股份有限公司 | Denitration automatic control system |
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