CN104641944A - Method for culturing cordyceps sinensis by taking unhulled wheat as culture medium - Google Patents
Method for culturing cordyceps sinensis by taking unhulled wheat as culture medium Download PDFInfo
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- CN104641944A CN104641944A CN201510119712.XA CN201510119712A CN104641944A CN 104641944 A CN104641944 A CN 104641944A CN 201510119712 A CN201510119712 A CN 201510119712A CN 104641944 A CN104641944 A CN 104641944A
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Abstract
The invention relates to a method for culturing cordyceps sinensis by taking unhulled wheat as a culture medium. The method comprises the following steps: preparing a culture medium by taking unhulled wheat grains as raw materials and cordyceps sinensis liquid strain as a strain, inoculating the wheat grain culture medium with the liquid strain, and culturing a cordyceps sinensis fruiting body. Applied wheat grain raw material sources are wide, and low in price; during the implementation, the inoculated cordyceps sinensis liquid strain use amount can be increased, and can penetrate into and be distributed into gaps among wheat grains due to no adhesion among the wheat grains, so that the medium is entirely contacted with the liquid strain, and hypha fully grows in the culture medium once the hypha germinates. In addition, steamed wheat grain hulls are cracked, wheat is exposed, the wheat is always wrapped by the hulls, so that the gelatinization and adhesion of the wheat can be effectively prevented and treated to influence the running and the growth of the hypha, the inoculated cordyceps sinensis strain can also directly contact with the wheatand absorb wheat nutrients; and the wheat is wrapped by the hulls, so that moisture is difficult to lose, and the sufficient moisture can guarantee the yield of the cordyceps sinensis.
Description
Technical field
The invention belongs to biology techniques field, be specifically related to a kind of method that culture matrix cultivation Chinese caterpillar fungus done by unhulled wheat.
Technical background
Chinese caterpillar fungus, be also called northern Chinese caterpillar Fungus, Cordyceps militaris, it is the complex that section ergot fungus cordyceps sinensis Cordycrps sinensis (Berk.) Sacc colonizes in stroma on larvae section insect larvae and larva corpse, be a kind of traditional famous and precious tonic Chinese herbal medicine material, be listed as three large invigorants with natural ginseng, pilose antler.Its property of medicine is gentle, throughout the year equal edible, and old or young, sick, weak, virtual person is all suitable, has medical value widely than the invigorant of other kinds.Since ancient times, people all using it as invigorant and pharmaceutical products.
Natural wild cordyceps primary growth is in High aititude, high and cold area, and the formation of growing environment is very harsh and complicated, and people constantly increase the demand of Chinese caterpillar fungus and irrationally to excavate in addition, cause natural Cordyceps sinensis natural resources to gradually reduce.In order to solve the deficiency of natural cordyceps, can only be met the need of market by artificial cultivation Chinese caterpillar fungus.In recent years; the domestic and international research to artificial cultivation Chinese caterpillar fungus and practical work extensively and are in depth carrying out; Cordyceps Militaris is infected host larva; as made infectosome with silkworm chrysalis polypide and to cultivate the technology of cordyceps militaris sporocarp ripe, utilize that this technology carries out batch production, there are plenty of such people in large-scale production.The price of current silkworm chrysalis is at 30 ~ 40 yuan/kilogram, and silkworm chrysalis can not year-round supply, and this has limited to anniversaryization and has produced; In addition, the inoculation link that silkworm chrysalis is cultivated in Chinese caterpillar fungus process is more loaded down with trivial details, and production efficiency is low.Along with technology constantly develops, use now the grain such as rice, wheat also very ripe as the technology of substrate culture Chinese caterpillar fungus, the application of this technology is popularized for various places.Current rice, Wheat price are 4 ~ 5 yuan/kilogram, and than silkworm chrysalis raw material, production cost significantly lowering clearly, with rice, wheat as substrate culture Chinese caterpillar fungus, also convenient, fast during inoculation, the efficiency of inoculation also improves a lot thus.But rice, wheat cook rear easy adhesion, during inoculation, bacterial classification infiltration is less than material the inside, and after mycelium germination, growing way is slow; In addition, the easy dehydration of rice, wheat, affects the output in Chinese caterpillar fungus later stage then.
Summary of the invention
Object of the present invention is exactly overcome the deficiencies in the prior art and provide a kind of unhulled wheat to make the method for culture matrix cultivation Chinese caterpillar fungus.Its feature comprises: be that primary raw material prepares culture matrix with unhulled wheat berry, with Chinese caterpillar fungus liquid spawn as bacterial classification, is received on wheat berry culture matrix by Chinese caterpillar fungus liquid spawn, and turns out cordyceps militaris sporocarp.Feature of the present invention is: the wheat berry raw material sources of application are wide, cheap; In implementation process, access Chinese caterpillar fungus liquid spawn dosage can be strengthened, due to adhesion between wheat berry, after inoculation, liquid spawn can be permeated completely and spread all over the gap between wheat berry, comprehensive engagement is to liquid spawn quickly to make matrix, and mycelia also just covers with mycelia very soon in germination medium.And with rice, shelling wheat as matrix, cook rear matrix adherence together, during inoculation, liquid spawn can only stay in matrix charge level, bacterial classification sprout after just slowly toward expect in material feeding, the process that mycelia eats up material is very long.In addition, the wheat berry shell cooked ftractures and exposes wheat, but shell encloses wheat all the time, such effectively preventing wheat gelatinization adhesion and affect mycelia and spread growth, but after access cordyceps species, bacterial classification also directly can contact and absorb the nutrient of wheat; Wheat is owing to being enclose by shell, and the moisture not easily evaporation water loss of wheat, sufficient moisture ensure that the output of Chinese caterpillar fungus.
Described wheat berry belongs to Triticum gramineous, is one of the widest, maximum, the total product of area is the highest, the volume of trade maximum, nutritive value the is the highest cereal crops that distribute in the world.Wheat kernel contains abundant starch, more protein, a small amount of fat, also has multi mineral prime element and Cobastab, is a kind of nutritious, commodity food that economic worth is higher.The main component of wheat is the compositions such as carbohydrate, fat, protein, raw fiber, calcium, phosphorus, potassium, vitamin B1, vitamin B2 and nicotinic acid, also has a kind of composition of allantoin.In addition, in wheat embryo, food fiber and vitamin E is also rich in, arginine, amylase, sitosterol, lecithin and protease that heart is a small amount of.Wheat is rich in starch, protein, fat, mineral matter, calcium, iron, thiamine, vitamin b3, nicotinic acid, vitamin A and vitamin C etc.
The present invention is achieved through the following technical solutions:
A kind of method doing culture matrix cultivation Chinese caterpillar fungus with unhulled wheat, technical scheme is: be that primary raw material prepares culture matrix with unhulled wheat berry, with Chinese caterpillar fungus liquid spawn as bacterial classification, Chinese caterpillar fungus liquid spawn is received on wheat berry culture matrix, and turns out cordyceps militaris sporocarp.
Specifically comprise the steps:
1, select to parch, go mouldy without damage by disease and insect, nothing, the normal and unhulled wheat berry of color and luster is as raw material.
2, select that mycelia size is even, energetic, good, the free of contamination Chinese caterpillar fungus liquid spawn of quality is as bacterial classification.
3, preparation production medium: raw material is by wheat berry 94%, corn flour 3%, water 3% forms, preparation process is first mixed in by water in corn flour and furnishing pasty state, wheat berry mixes and is stirred into medium again, then medium is sub-packed in culture vessel, charge weight is the thickness of medium in culture vessel is 2 ~ 3cm, and add the nutrient solution prepared routinely, the part by weight of nutrient solution and medium is 1.2 ~ 1.5: 1, finally the culture vessel that medium is housed is placed in high-pressure bottle, carry out disinfection to obtain production medium according to a conventional method.
4, inoculate: be transferred to by Chinese caterpillar fungus liquid spawn in an aseptic environment equably on the medium charge level of sterilization, inoculum concentration is the liquid spawn of the part by weight access 20 ~ 25% according to medium.
5, cultivate: the culture vessel to connect kind is moved on to environmental temperature is 20 ~ 23 DEG C, relative moisture is 60 ~ 65% culturing room's lucifuge and cultivate training and cover with mycelia to supporting base.
6, grass management is gone out: after medium mycelia is covered with, expose 2 ~ 3 days under culture vessel being moved on to the light conditions of 800 ~ 1000 luxs, mycelia is made to transfer salmon pink to, when there is the former base of small rice grain in the mycelia cultivating salmon pink on charge level, worm is described, and the grass is coming up, at this moment carries out routinely grass management.
Compared with prior art, the present invention both had the following advantages:
1, proposed by the invention to make the method for culture matrix cultivation Chinese caterpillar fungus with unhulled wheat not only novel, and measure is unique, and technique simply, is easily implemented.
2, primary raw material---the wheat berry used in the invention process process, raw material is extensive, cheap, than raw materials such as rice, shelling wheats, applies its production cost lower.
3, the present invention is in implementation process, access Chinese caterpillar fungus liquid spawn dosage can be strengthened, due to adhesion between wheat berry, after inoculation, liquid spawn can be permeated completely and spread all over the gap between wheat berry, comprehensive engagement is to liquid spawn quickly to make matrix, and mycelia also just covers with mycelia very soon in germination medium.
4, the present invention is in implementation process, the wheat berry shell cooked ftractures and exposes wheat, but shell encloses wheat all the time, such effectively preventing wheat gelatinization adhesion and affect mycelia and spread growth, but after access cordyceps species, bacterial classification also directly can contact and absorb the nutrient of wheat; Wheat is owing to being enclose by shell, and the moisture not easily evaporation water loss of wheat, sufficient moisture ensure that the output of Chinese caterpillar fungus.
5, by implementing the present invention, shorten Chinese caterpillar fungus cultivation cycle, worm grass product production cost reduces, and is worth promoting.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
A kind of method doing culture matrix cultivation Chinese caterpillar fungus with unhulled wheat, technical scheme is: be that primary raw material prepares culture matrix with unhulled wheat berry, with Chinese caterpillar fungus liquid spawn as bacterial classification, Chinese caterpillar fungus liquid spawn is received on wheat berry culture matrix, and turns out cordyceps militaris sporocarp.
Embodiment is as follows:
1, select to parch, go mouldy without damage by disease and insect, nothing, the normal and unhulled wheat berry of color and luster is as raw material.
2, select that mycelia size is even, energetic, good, the free of contamination Chinese caterpillar fungus liquid spawn of quality is as bacterial classification.
3, preparation production medium: raw material is by wheat berry 94%, corn flour 3%, water 3% forms, preparation process is first mixed in by water in corn flour and furnishing pasty state, wheat berry mixes and is stirred into medium again, then medium is sub-packed in culture vessel, charge weight is the thickness of medium in culture vessel is 2 ~ 3cm, and add the nutrient solution prepared routinely, the part by weight of nutrient solution and medium is 1.2 ~ 1.5: 1, finally the culture vessel that medium is housed is placed in high-pressure bottle, carry out disinfection to obtain production medium according to a conventional method.
4, inoculate: be transferred to by Chinese caterpillar fungus liquid spawn in an aseptic environment equably on the medium charge level of sterilization, inoculum concentration is the liquid spawn of the part by weight access 20 ~ 25% according to medium.
5, cultivate: the culture vessel to connect kind is moved on to environmental temperature is 20 ~ 23 DEG C, relative moisture is 60 ~ 65% culturing room's lucifuge and cultivate training and cover with mycelia to supporting base.
6, grass management is gone out: after medium mycelia is covered with, expose 2 ~ 3 days under culture vessel being moved on to the light conditions of 800 ~ 1000 luxs, mycelia is made to transfer salmon pink to, when there is the former base of small rice grain in the mycelia cultivating salmon pink on charge level, worm is described, and the grass is coming up, at this moment carries out routinely grass management.
Claims (1)
1. make the method for culture matrix cultivation Chinese caterpillar fungus with unhulled wheat for one kind, it is characterized in that: be that primary raw material prepares culture matrix with unhulled wheat berry, with Chinese caterpillar fungus liquid spawn as bacterial classification, Chinese caterpillar fungus liquid spawn is received on wheat berry culture matrix, and turns out cordyceps militaris sporocarp;
Specifically comprise the steps:
(1) select to parch, go mouldy without damage by disease and insect, nothing, the normal and unhulled wheat berry of color and luster is as raw material;
(2) select that mycelia size is even, energetic, good, the free of contamination Chinese caterpillar fungus liquid spawn of quality is as bacterial classification;
(3) production medium is prepared: raw material is by wheat berry 94%, corn flour 3%, water 3% forms, preparation process is first mixed in by water in corn flour and furnishing pasty state, wheat berry mixes and is stirred into medium again, then medium is sub-packed in culture vessel, charge weight is the thickness of medium in culture vessel is 2 ~ 3cm, and add the nutrient solution prepared routinely, the part by weight of nutrient solution and medium is 1.2 ~ 1.5: 1, finally the culture vessel that medium is housed is placed in high-pressure bottle, carry out disinfection to obtain production medium according to a conventional method,
(4) inoculate: be transferred to by Chinese caterpillar fungus liquid spawn in an aseptic environment equably on the medium charge level of sterilization, inoculum concentration is the liquid spawn of the part by weight access 20 ~ 25% according to medium;
(5) cultivate: the culture vessel to connect kind is moved on to environmental temperature is 20 ~ 23 DEG C, relative moisture is 60 ~ 65% culturing room's lucifuge and cultivate training and cover with mycelia to supporting base;
(6) grass management is gone out: after medium mycelia is covered with, expose 2 ~ 3 days under culture vessel being moved on to the light conditions of 800 ~ 1000 luxs, mycelia is made to transfer salmon pink to, when there is the former base of small rice grain in the mycelia cultivating salmon pink on charge level, worm is described, and the grass is coming up, at this moment carries out routinely grass management.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105315043A (en) * | 2015-12-03 | 2016-02-10 | 邢台学院 | Cordyceps militaris cultivation medium |
| JP2017200475A (en) * | 2016-05-03 | 2017-11-09 | メディネクス株式会社 | Method for producing mixture of parasitic mushroom fungi and culture medium |
| CN108077904A (en) * | 2017-12-21 | 2018-05-29 | 山西佰富康生物科技有限公司 | A kind of Cordyceps militaris nutrition brewed powder and preparation method thereof |
| CN112831086A (en) * | 2021-01-18 | 2021-05-25 | 刘永昶 | Preparation method and application of special solid strain for liquid fermentation culture |
| CN114885748A (en) * | 2022-06-10 | 2022-08-12 | 皖北卫生职业学院 | Rapid culture method of cordyceps sobolifera coreopsis |
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| CN1772876A (en) * | 2005-09-30 | 2006-05-17 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
| CN101440389A (en) * | 2008-10-23 | 2009-05-27 | 贵州大学 | Method for increasing cordycepin content in Cordceps militaris solid culture medium |
| CN101463325A (en) * | 2008-12-16 | 2009-06-24 | 浙江博士园生物技术有限公司 | Industrialized cultivation method for north aweto |
| CN102550300A (en) * | 2012-03-10 | 2012-07-11 | 何寒 | Method for cultivating cordyceps sinensis by taking unhusked rice as culture medium |
| CN103461005A (en) * | 2013-09-29 | 2013-12-25 | 凌爱秋 | Method for preparing cordyceps sinensis block by germinant wheat |
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2015
- 2015-03-19 CN CN201510119712.XA patent/CN104641944A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1772876A (en) * | 2005-09-30 | 2006-05-17 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
| CN101440389A (en) * | 2008-10-23 | 2009-05-27 | 贵州大学 | Method for increasing cordycepin content in Cordceps militaris solid culture medium |
| CN101463325A (en) * | 2008-12-16 | 2009-06-24 | 浙江博士园生物技术有限公司 | Industrialized cultivation method for north aweto |
| CN102550300A (en) * | 2012-03-10 | 2012-07-11 | 何寒 | Method for cultivating cordyceps sinensis by taking unhusked rice as culture medium |
| CN103461005A (en) * | 2013-09-29 | 2013-12-25 | 凌爱秋 | Method for preparing cordyceps sinensis block by germinant wheat |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105315043A (en) * | 2015-12-03 | 2016-02-10 | 邢台学院 | Cordyceps militaris cultivation medium |
| JP2017200475A (en) * | 2016-05-03 | 2017-11-09 | メディネクス株式会社 | Method for producing mixture of parasitic mushroom fungi and culture medium |
| CN108077904A (en) * | 2017-12-21 | 2018-05-29 | 山西佰富康生物科技有限公司 | A kind of Cordyceps militaris nutrition brewed powder and preparation method thereof |
| CN112831086A (en) * | 2021-01-18 | 2021-05-25 | 刘永昶 | Preparation method and application of special solid strain for liquid fermentation culture |
| CN114885748A (en) * | 2022-06-10 | 2022-08-12 | 皖北卫生职业学院 | Rapid culture method of cordyceps sobolifera coreopsis |
| CN114885748B (en) * | 2022-06-10 | 2023-06-20 | 皖北卫生职业学院 | Rapid culture method of cordyceps sobolifera stem bundles |
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