CN105613031A - Method of preparing cordyceps sinensis masses by adoption of germinated corns - Google Patents
Method of preparing cordyceps sinensis masses by adoption of germinated corns Download PDFInfo
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- CN105613031A CN105613031A CN201410444358.3A CN201410444358A CN105613031A CN 105613031 A CN105613031 A CN 105613031A CN 201410444358 A CN201410444358 A CN 201410444358A CN 105613031 A CN105613031 A CN 105613031A
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Abstract
Provided is a method of preparing cordyceps sinensis masses by adoption of germinated corns. The method comprises following steps: utilizing germinated corns as raw material; utilizing corn flour and sugar to prepare a nutrient solution without inorganic salt; utilizing liquid strains of cordyceps sinensis as strains to be connected to a culture medium used for production and composed of germinated corns and the nutrient solution without inorganic salt; and finally culturing till the culture medium are interwoven to masses by cordyceps sinensis in order to obtain germinated kernel cordyceps sinensis mass products. By applying the invention, germinated corns are processed by germination of ordinary corns so that enrichment changes are made on nutrient content. A combination with another feature, germinated corn cordyceps sinensis masses, is formed by cordyceps sinensis and germinated corns. Therefore, nutrition, functions and efficacy are better and products are beautiful in appearances and are novel and unique and taste good and tend to be the favorite of people; corns therefore can see over 10-fold increase. The method is featured by being short in culture period, easy in production and preparation technology, low in production cost of products, high in production efficiency of products and is stable.
Description
Technical field
The preparation method that the present invention relates to a kind of functional living being goods, belongs to biological technical field, is specifically related to a kind of method that Cordyceps mycelium block prepared by germination Semen Maydis.
Technical background
Cordyceps militaris (L.) Link., also known as Cordyceps militaris (L.) Link., it is that section ergot fungus cordyceps sinensis Cordycrpssinensis (Berk.) Sacc colonizes in the Stroma on larvae section insect larvae and larva cadaveric complex, it is a kind of traditional famous and precious tonic Chinese medicine material, is listed as three big tonic with natural ginseng, Cornu Cervi Pantotrichum. Its property of medicine is gentle, throughout the year equal edible, and old or young, sick, weak, virtual person is all suitable, has medical value widely than other kinds of tonic. Since ancient times, people all using it as tonic and pharmaceutical products.
Natural wild cordyceps primary growth is at High aititude, extremely frigid zones, and the composition of growing environment is very harsh and complicated, and the demand of Cordyceps is continuously increased and irrational excavates by people in addition, causes natural Cordyceps natural resources to gradually reduce. Therefore, in order to solve medicine source, scientific and technical personnel have to meet the market demand by Artificial Cultivation of Cordyceps militaris Link, but for these years, tame Cordyceps militaris (L.) Link. complex manufacturing, production cycle length, yield poorly etc. annoyings people always.
Along with deepening continuously of technical research, scientific research personnel goes out brand-new a kind of worm grass product Cordyceps mycelium with biological submerged fermentation technological development. Cordyceps mycelium had once been carried out the analysis of effective ingredient by Agricultural University Of Anhui, its result shows: in mycelium, amino acid classes is consistent with Cordyceps crude drug, but total amino acid content and TEAA are apparently higher than Cordyceps crude drug, essential amino acids accounts for the ratio of total amino acids also above the latter, threonine in essential amino acids, valine, isoleucine, leucine, lysine content farther far above the latter, in mycelium, the content of crude protein, total sugar and mannitol is above Cordyceps crude drug; In mycelial fatty acid composition, unsaturated fatty acid accounts for the 85.38% of fatty acid composition, its polyvalent unsaturated fatty acid such as Linoleic acid, linolenic acid accounts for the 58.87% of fatty acid composition, and linoleic content accounts for more than half (51.04%) of whole fatty acid composition; Fermentation mycelium possibly together with abundant to metallic elements such as health very important Zn, Fe, Ca, especially with the content of Ca for height.
Producing Cordyceps mycelium by biological submerged fermentation technology, the features such as it is with short production cycle, technique is simple, production efficiency is high and stable, are the main development directions of artificial culture worm grass product. At present the raw material produced used by Cordyceps mycelium is mainly grain by-product, and such as Testa oryzae, wheat bran etc., adding a little mineral element on this raw material basis is carbon and nitrogen sources, mycelium cultivate after again through drying and being ground into powder. But, can be run off to some extent by its nutritional labeling of mycelium dried. It addition, except the function having except product itself with such explained hereafter mycelium product out, effect, the poor morphology of its product formation, mouthfeel are not good, even without any taste, it is impossible to attract much attention, so product promotion poor effect.
Summary of the invention
The purpose of the present invention is exactly overcome the deficiency of above-mentioned technology and the method that provides brand-new a kind of germination Semen Maydis to prepare Cordyceps mycelium block. Specifically include: with germination Semen Maydis as raw material, the nutritional solution of inorganic salt it is prepared without with Semen Maydis powder and sugar, as strain and access by germination Semen Maydis and the production culture medium that form without mineral nutrition liquid with Cordyceps liquid spawn, be finally cultured to the Cordyceps mycelium connection in bulk that culture medium interweaved and must germinate wheat grain Cordyceps mycelium block finished product. By implement the present invention, germination Semen Maydis used be with conventional corn after germination treatment so that it is nutritional labeling generation enriching change. Cordyceps mycelium and germination Semen Maydis form the coalition germination Semen Maydis Cordyceps mycelium block of another characteristic so that it is not only can be more prominent in nutrition, function and effect, and the formal beauty of product, novelty, characteristic, and the good grade of mouthfeel will be the new lovers that consume of people; Semen Maydis also thus rises in value decades of times. The present invention also has the features such as cultivation period is short, process for producing simple, the production cost of product is low, the production efficiency of product is high and stable.
The present invention is achieved through the following technical solutions:
A kind of method that Cordyceps mycelium block prepared by germination Semen Maydis, comprises the steps: in technical scheme
1, material choice: select free from admixture, without going mouldy, free from extraneous odour, color and luster be normal, moisture lower than 18��20% and germination percentage be more than 95% germination niblet be raw material.
2, prepare nutritional solution: raw material composition be 0.38% Semen Maydis powder, 0.22% sugar, 99.4% water, preparation process be water heating before first Semen Maydis powder and sugar are mixed in water, be again heated to solution boiling after maintain 3��5 minutes nutritional solution.
3, preparation production culture medium: raw material is made up of the germination niblet of 50% and the nutritional solution of 50%, preparation process is to be sub-packed in culture vessel by germination niblet, charge weight is germination niblet thickness in culture vessel is 2��3cm, and by the part by weight interpolation nutritional solution of loaded germination niblet, finally carry out disinfection sterilizing according to a conventional method and must produce and use culture medium.
4, strain selects: select to cultivate the mycelia of gained according to a conventional method and fungus ball size is uniform, energetic, quality Cordyceps liquid spawn good, free of contamination is as kind of a source.
5, inoculation: access the Cordyceps liquid spawn of 20��25% in an aseptic environment according to the weight of culture medium.
6, mycelia block is cultivated: move on to culturing room's lucifuge cultivation by connecting the culture vessel planted, temperature be 20��23 DEG C, relative humidity be 60��65% environment under cultivate 4��5 days, when mycelia covers with culture medium, culture medium is interweaved by mycelia and connects in bulk and must germinate niblet Cordyceps mycelium block finished product.
The present invention both had the following advantages:
1, germination niblet Cordyceps mycelium block preparation method technique proposed by the invention is simply, easily implement.
2, by implementing the present invention, raw material germination Semen Maydis used, it is simply that with conventional corn after germination treatment so that it is nutritional labeling generation enriching changes. Utilize germination Semen Maydis as culture matrix, Cordyceps mycelium is cultivated afterwards and germination Semen Maydis forms the coalition germination Semen Maydis Cordyceps mycelium block of another characteristic, make it not only can be more prominent in nutrition, function and effect, and the formal beauty of product, novelty, characteristic, the good grade of mouthfeel will be the new lovers that consume of people; Semen Maydis also thus increment decades of times even Radix Achyranthis Bidentatae.
3, utilize plant nutrient source to be prepared without the nutritional solution of inorganic salt as carbon and nitrogen sources, ensured that the formation of mycelium product is without source of heavy metal pollution, has possessed natural health, green health.
4, by implementing the present invention, the process for producing of final products Cordyceps mycelium block, there is the features such as cultivation period is short, process for producing simple, the production cost of product is low, the production efficiency of product is high and stable.
5, by implementing the present invention, final products Cordyceps mycelium block, the features such as the formation of product is characteristic, novel, nutritive value doubles, mouthfeel is good, also there is special food therapy function simultaneously; Formed product can be cold and dressed with sauce, fry, stew etc. method processing and eating, be alternatively arranged as the raw material preparing functional rice, mycelium, canned food, puffed food, functional characteristic snack etc. series of products. Producing Cordyceps mycelium block, market prospect is good, remarkable benefit.
Detailed description of the invention
Below in conjunction with embodiment, the method for the present invention is further illustrated.
A kind of method that Cordyceps mycelium block prepared by germination Semen Maydis, specific implementation method is as follows:
1, material choice: select free from admixture, without going mouldy, free from extraneous odour, color and luster be normal, moisture lower than 18��20% and germination percentage be more than 95% germination niblet be raw material.
2, prepare nutritional solution: raw material composition be 0.38% Semen Maydis powder, 0.22% sugar, 99.4% water, preparation process be water heating before first Semen Maydis powder and sugar are mixed in water, be again heated to solution boiling after maintain 3��5 minutes nutritional solution.
3, preparation production culture medium: raw material is made up of the germination niblet of 50% and the nutritional solution of 50%, preparation process is to be sub-packed in culture vessel by germination niblet, charge weight is germination niblet thickness in culture vessel is 2��3cm, and by the part by weight interpolation nutritional solution of loaded germination niblet, finally carry out disinfection sterilizing according to a conventional method and must produce and use culture medium.
4, strain selects: select to cultivate the mycelia of gained according to a conventional method and fungus ball size is uniform, energetic, quality Cordyceps liquid spawn good, free of contamination is as kind of a source.
5, inoculation: access the Cordyceps liquid spawn of 20��25% in an aseptic environment according to the weight of culture medium. 6, mycelia block is cultivated: move on to culturing room's lucifuge cultivation by connecting the culture vessel planted, temperature be 20��23 DEG C, relative humidity be 60��65% environment under cultivate 4��5 days, when mycelia covers with culture medium, culture medium is interweaved by mycelia and connects in bulk and must germinate niblet Cordyceps mycelium block finished product.
Claims (1)
1. the method preparing Cordyceps mycelium block with germination Semen Maydis, its feature comprises the steps:
(1) material choice: select free from admixture, without going mouldy, free from extraneous odour, color and luster be normal, moisture lower than 18��20% and germination percentage be more than 95% germination niblet be raw material;
(2) prepare nutritional solution: raw material composition be 0.38% Semen Maydis powder, 0.22% sugar, 99.4% water, preparation process be water heating before first Semen Maydis powder and sugar are mixed in water, be again heated to solution boiling after maintain 3��5 minutes nutritional solution;
(3) production culture medium is prepared: raw material is made up of the germination niblet of 50% and the nutritional solution of 50%, preparation process is to be sub-packed in culture vessel by germination niblet, charge weight is germination niblet thickness in culture vessel is 2��3cm, and by the part by weight interpolation nutritional solution of loaded germination niblet, finally carry out disinfection sterilizing according to a conventional method and use culture medium must be produced;
(4) strain selects: select to cultivate the mycelia of gained according to a conventional method and fungus ball size is uniform, energetic, quality Cordyceps liquid spawn good, free of contamination is as kind of a source;
(5) inoculation: access the Cordyceps liquid spawn of 20��25% in an aseptic environment according to the weight of culture medium;
(6) mycelia block is cultivated: move on to culturing room's lucifuge cultivation by connecting the culture vessel planted, temperature be 20��23 DEG C, relative humidity be 60��65% environment under cultivate 4��5 days, when mycelia covers with culture medium, culture medium is connected in bulk by mycelia intertexture must send out niblet Cordyceps mycelium block finished product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105900695A (en) * | 2016-06-28 | 2016-08-31 | 黄秀英 | Cordyceps sinensis mycelium prepared from dendrobium officinale as culture medium |
| CN106107974A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | A kind of Fructus Jujubae, Fructus Lycii, Cordyceps combination mycelia block |
| CN106360290A (en) * | 2016-08-26 | 2017-02-01 | 黄爱红 | Preparation method of cordyceps militaris corn flour |
-
2014
- 2014-09-02 CN CN201410444358.3A patent/CN105613031A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105900695A (en) * | 2016-06-28 | 2016-08-31 | 黄秀英 | Cordyceps sinensis mycelium prepared from dendrobium officinale as culture medium |
| CN106107974A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | A kind of Fructus Jujubae, Fructus Lycii, Cordyceps combination mycelia block |
| CN105900695B (en) * | 2016-06-28 | 2019-03-01 | 广西壮仁堂生物科技有限公司 | Cordyceps mycelia is prepared by culture medium of dendrobium candidum |
| CN106360290A (en) * | 2016-08-26 | 2017-02-01 | 黄爱红 | Preparation method of cordyceps militaris corn flour |
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Application publication date: 20160601 |