CN109105148A - A kind of high yield and high quality cordyceps militaris plantation culture medium and its cultural method - Google Patents
A kind of high yield and high quality cordyceps militaris plantation culture medium and its cultural method Download PDFInfo
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- CN109105148A CN109105148A CN201810950967.4A CN201810950967A CN109105148A CN 109105148 A CN109105148 A CN 109105148A CN 201810950967 A CN201810950967 A CN 201810950967A CN 109105148 A CN109105148 A CN 109105148A
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- culture medium
- cordyceps militaris
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/70—Harvesting
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- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention provides a kind of high yield and high quality cordyceps militaris plantation culture medium and its cultural methods, the culture medium is with rice or wheat for main cultivation matrix, add rice or corncob, wheat bran, sawdust or the husk of wheat quality 10%-40%, mixed liquor is added than the nutrient solution for 1:1.1-1:1.5g/mL, culture medium for cultivating is made after sterilizing.The Cordyceps militaris cultivated using basal culture medium, compared with traditional 100% rice cultivation, faster, yield is higher for mycelia delivery speed, and sporophore growth more evenly, it is healthy and strong.On the other hand the ratio for reducing cereal crops, reduces costs, increases economic efficiency.
Description
Technical field
The present invention relates to field of edible fungus culture, and in particular to a kind of high yield and high quality cordyceps militaris plantation culture medium and its cultivation
Method.
Background technique
Cordyceps militaris also known as northern Chinese caterpillar Fungus, Cordceps militaris belong to Eumycota (Fungi), Ascomycetes (Ascomycota), meat
Seat Zoopagales (Hypocreales), Clavicipitaceae (Clavicipitaceae), Cordyceps (Cordyceps), divide in nature
Cloth is extensive.Because there is antibacterial, anti-inflammatory, antitumor, hypoglycemic containing cordycepin, cordycepic acid, adenosine and polysaccharide isoreactivity ingredient due to
And the remarkable effects such as immune function of human body are adjusted, it is a kind of dual-purpose of drug and food fungi with tonic effect, there is very high medicinal valence
Value and nutritive value, are received, demand also rises year by year by more and more consumers.But cultural method is without very big prominent
Broken, the cultivation of Cordyceps militaris is generally with the cultivation of insect pupal cell or grain husbandry, wherein using cereal such as 100% rice, wheats as cultivation
Based on culture medium, but cereal crops can all expand after cooking or being cooked, and cause culture medium poor air permeability, mycelia delivery speed
Slowly, material feeding is shallow, and nutritional utilization is insufficient, and biological transformation ratio is not high enough.
Summary of the invention
It is an object of the invention to find a kind of method of improvement grain culture medium cultivation Cordyceps militaris, so that Cordyceps militaris grows
Faster, the production cycle shortens speed, and biological efficiency is higher, the lower cordyceps militaris plantation culture medium of culture medium cost of manufacture.
To solve the above problems, the present invention adopts the following technical scheme:
A kind of high yield and high quality cordyceps militaris plantation culture medium, the culture medium prescription are as follows: with rice or wheat for main cultivation base
Matter adds rice or corncob, wheat bran, sawdust or the husk of wheat quality 10%-40%, and adding mixed liquor ratio is 1:1.1-
Culture medium for cultivating is made after sterilizing in the nutrient solution of 1:1.5 g/mL.Wherein nutrient solution prescription is calculated by mass percentage are as follows: phosphoric acid
Potassium dihydrogen 0.15% ~ 0.25%, magnesium sulfate 0.1% ~ 0.2%, calcium carbonate 0.1% ~ 0.18%, vitamin B1 0.0005%-0.0015%,
Surplus is water, and the sum of each component is 100%.
A kind of high yield and high quality cordyceps militaris plantation method, comprising the following steps:
(1) compounding: weighing potassium dihydrogen phosphate, magnesium sulfate, calcium carbonate and vitamin B1, is completely dissolved in water and battalion is made
Nutrient solution, nutrient solution prescription are calculated by mass percentage are as follows: potassium dihydrogen phosphate 0.15% ~ 0.25%, magnesium sulfate 0.1% ~ 0.2%, carbonic acid
Calcium 0.1% ~ 0.18%, vitamin B1 0.0005%-0.0015%, surplus are water, and the sum of each component is 100%；
(2) preparation of culture medium: weighing a certain amount of rice or wheat, rice or corncob, the wheat of wheat quality 10-40% is added
Bran, sawdust or husk, then step (1) prepared nutrient solution is added by mixed liquor ratio 1:1.1-1:1.5 g/mL, mixing is equal
Packing will be cultivated box 121 DEG C of sterilizing 20min in high-pressure sterilizing pot, taken out spare into cultivation box after even；
(3) it is inoculated with: after culture medium is cooled to room temperature, moving into aseptic operating platform inoculation as early as possible, first use Cordyceps militaris strain
Sterile water dilution, then the strain of 7-10%vol is accessed into culture medium with liquid spawn inoculator；
(4) bacterium germination: by the media transfer after inoculation to the bacteria-producing room being protected from light, controlling 20-25 DEG C of temperature, cultivates 3d, and mycelia will
Cover with matrix；
(5) management of producing mushroom: removing bacteria-producing room for the cultivation box of the good mycelia of length, opens light and carries out lighting process, intensity of illumination is
300lx keeps daily illumination 10-14h, to the thin out yellow of mycelia to orange-yellow, the sealing cover of opening cultivation box, holding air phase
To humidity in 85%-95%, temperature is controlled during 16-24 DEG C, flower bud, guarantees 5 DEG C of the temperature difference to stimulate former base to break up；
(6) harvest: when sporophore growth tends to stop, surface starts the shell of ascus for powdery occur, is Harvesting Date, and harvesting obtains
High-quality Cordyceps militaris.
Remarkable advantage of the invention:
The present invention provides a kind of method of improvement grain culture medium cultivation Cordyceps militaris, so that the Cordyceps militaris speed of growth is faster, production
Cycle time, biological efficiency is higher, the lower cordyceps militaris plantation culture medium of culture medium cost of manufacture.
The Cordyceps militaris cultivated using culture medium of the present invention, compared with traditional 100% rice cultivation, mycelia delivery speed is more
Fastly, yield is higher, and sporophore growth more evenly, it is healthy and strong.On the other hand the ratio for reducing cereal crops, reduces into
This, increases economic efficiency.
Detailed description of the invention
Fig. 1 is the growth figure that rice husk culture medium cultivates Cordyceps militaris.
Specific embodiment
Embodiment 1: rice husk cultivates Cordyceps militaris test
The husk of rice quality 10%-40% is mixed in rice medium, then is added and is sought in mixed liquor ratio 1:1.4g/mL ratio
Cordyceps militaris plantation culture medium is made in nutrient solution, and the formula of the nutrient solution is as follows by mass percentage: potassium dihydrogen phosphate 0.2%, sulphur
Sour magnesium 0.1%, calcium carbonate 0.1% and vitamin B1 0.001%, surplus are water, and the sum of each component is 100%.
Its specific implementation step is as follows:
(1) compounding: potassium dihydrogen phosphate, magnesium sulfate, calcium carbonate and vitamin B1 are weighed according to above-mentioned nutrient solution prescription, is filled
Divide to be dissolved in the water and nutrient solution is made；
(2) preparation of culture medium: weighing a certain amount of rice, a certain amount of husk is weighed in proportion, so that the quality of husk accounts for greatly
The ratio of meter Zhi Liang is respectively 10%, 20% and 40%, then above-mentioned prepared nutrient solution, mixing is added by solid-liquid ratio 1:1.4g/mL
Packing will be cultivated box 121 DEG C of sterilizing 20min in high-pressure sterilizing pot, taken out spare into cultivation box after uniformly；
(3) it is inoculated with: after culture medium is cooled to room temperature, moving into aseptic operating platform inoculation as early as possible.Cordyceps militaris strain is used first
Sterile water is diluted to proper density, then accesses the strain of 8%vol into culture medium with liquid spawn inoculator；
(4) bacterium germination: by the media transfer after inoculation to the bacteria-producing room being protected from light, 25 DEG C of temperature are controlled, culture 3d or so, mycelia will
Cover with matrix；
(5) management of producing mushroom: removing bacteria-producing room for the cultivation box of the good mycelia of length, opens light and carries out lighting process, intensity of illumination is
300lx keeps daily illumination 12h, and to the thin out yellow of mycelia to orange-yellow, the sealing cover of opening cultivation box, holding air is opposite
Humidity controls temperature during 16-24 DEG C, flower bud in 85%-95%, guarantees 5 DEG C or so of the temperature difference to stimulate former base to break up；
(6) harvest: when sporophore growth tends to stop, surface starts the shell of ascus for powdery occur, is Harvesting Date；
(7) record data: observation sporophore growth situation (Fig. 1) records yield data.
Embodiment 2: rice corncob cultivation Cordyceps militaris test
The corncob of rice quality 10%-40% is mixed in rice medium, then is added in mixed liquor ratio 1:1.4g/mL ratio
Cordyceps militaris plantation culture medium is made in nutrient solution, and the formula of the nutrient solution is as follows by mass percentage: potassium dihydrogen phosphate 0.2%,
Magnesium sulfate 0.1%, calcium carbonate 0.1% and vitamin B1 0.001%, surplus are water, and the sum of each component is 100%.
Its specific implementation step is as follows:
(1) compounding: potassium dihydrogen phosphate, magnesium sulfate, calcium carbonate and vitamin B1 are weighed according to above-mentioned nutrient solution prescription, is filled
Divide to be dissolved in the water and nutrient solution is made；
(2) preparation of culture medium: weighing a certain amount of rice, then by the above-mentioned prepared nutrition of solid-liquid ratio 1:1.4 g/mL addition
Rice is cooked with electric cooker or other steamed rice instruments for liquid, spare after slightly cooling down；A certain amount of corncob is weighed, then presses feed liquid
Above-mentioned prepared nutrient solution is added than 1:1.4g/mL, stirs evenly, after corncob fully absorbs nutrient solution, takes in proportion
Corncob and cooked rice, so that the ratio that the quality of corncob accounts for cooked rice quality is respectively 10%, 20% and 40%,
It dispenses after mixing into culture bottle, per bottled 30g, 121 DEG C of sterilizings in high-pressure sterilizing pot by culture bottle after being slightly compacted
20min takes out spare；
(3) it is inoculated with: after culture medium is cooled to room temperature, moving into aseptic operating platform inoculation as early as possible.Cordyceps militaris strain is used first
Sterile water is diluted to proper density, then accesses the strain of 8%vol into culture medium with liquid spawn inoculator；
(4) bacterium germination: by the media transfer after inoculation to the bacteria-producing room being protected from light, 25 DEG C of temperature are controlled, culture 3d or so, mycelia will
Cover with matrix；
(5) management of producing mushroom: removing bacteria-producing room for the cultivation box of the good mycelia of length, opens light and carries out lighting process, intensity of illumination is
300lx keeps daily illumination 12h, and to the thin out yellow of mycelia to orange-yellow, the sealing cover of opening cultivation box, holding air is opposite
Humidity controls temperature during 16-24 DEG C, flower bud in 85%-95%, guarantees 5 DEG C or so of the temperature difference to stimulate former base to break up；
(6) harvest: when sporophore growth tends to stop, surface starts the shell of ascus for powdery occur, is Harvesting Date；
(7) record data: observation sporophore growth situation records yield data.
Embodiment 3: rice wheat bran cultivates Cordyceps militaris test
The wheat bran of rice quality 10%-40% is mixed in rice medium, then is added and is sought in mixed liquor ratio 1:1.4g/mL ratio
Cordyceps militaris plantation culture medium is made in nutrient solution, and the formula of the nutrient solution is as follows by mass percentage: potassium dihydrogen phosphate 0.2%, sulphur
Sour magnesium 0.1%, calcium carbonate 0.1% and vitamin B1 0.001%, surplus are water, and the sum of each component is 100%.
Its specific implementation step is as follows:
(1) compounding: potassium dihydrogen phosphate, magnesium sulfate, calcium carbonate and vitamin B1 are weighed according to above-mentioned nutrient solution prescription, is filled
Divide to be dissolved in the water and nutrient solution is made；
(2) it preparation of culture medium: weighing a certain amount of rice, then presses solid-liquid ratio 1:1.4g/mL and above-mentioned prepared nutrient solution is added,
Rice is cooked with electric cooker or other steamed rice instruments, it is spare after slightly cooling down；A certain amount of wheat bran is weighed, then presses solid-liquid ratio 1:
Above-mentioned prepared nutrient solution is added in 1.4g/mL, stirs evenly, after wheat bran fully absorbs nutrient solution, take in proportion wheat bran with
Cooked rice, so that the ratio that the quality of wheat bran accounts for cooked rice quality is respectively 10%, 20% and 40%, after mixing
Packing is into culture bottle, and per bottled 30g, by culture bottle, 121 DEG C of sterilizing 20min, taking-up are standby in high-pressure sterilizing pot after being slightly compacted
With；
(3) it is inoculated with: after culture medium is cooled to room temperature, moving into aseptic operating platform inoculation as early as possible.Cordyceps militaris strain is used first
Sterile water is diluted to proper density, then accesses the strain of 8%vol into culture medium with liquid spawn inoculator；
(4) bacterium germination: by the media transfer after inoculation to the bacteria-producing room being protected from light, 25 DEG C of temperature are controlled, culture 3d or so, mycelia will
Cover with matrix；
(5) management of producing mushroom: removing bacteria-producing room for the cultivation box of the good mycelia of length, opens light and carries out lighting process, intensity of illumination is
300lx keeps daily illumination 12h, and to the thin out yellow of mycelia to orange-yellow, the sealing cover of opening cultivation box, holding air is opposite
Humidity controls temperature during 16-24 DEG C, flower bud in 85%-95%, guarantees 5 DEG C or so of the temperature difference to stimulate former base to break up；
(6) harvest: when sporophore growth tends to stop, surface starts the shell of ascus for powdery occur, is Harvesting Date；
(7) record data: observation sporophore growth situation records yield data.
Embodiment 4: rice cultivation on sawdust Cordyceps militaris test
The sawdust of rice quality 10%-40% is mixed in rice medium, then is added and is sought in mixed liquor ratio 1:1.4g/mL ratio
Cordyceps militaris plantation culture medium is made in nutrient solution, and the formula of the nutrient solution is as follows by mass percentage: potassium dihydrogen phosphate 0.2%, sulphur
Sour magnesium 0.1%, calcium carbonate 0.1% and vitamin B1 0.001%, surplus are water, and the sum of each component is 100%.
Its specific implementation step is as follows:
(1) compounding: potassium dihydrogen phosphate, magnesium sulfate, calcium carbonate and vitamin B1 are weighed according to above-mentioned nutrient solution prescription, is filled
Divide to be dissolved in the water and nutrient solution is made；
(2) preparation of culture medium: weighing a certain amount of rice, a certain amount of sawdust is weighed in proportion, so that the quality of sawdust accounts for greatly
The ratio of meter Zhi Liang is respectively 10%, 20% and 40%, then above-mentioned prepared nutrient solution, mixing is added by solid-liquid ratio 1:1.4g/mL
Packing will be cultivated box 121 DEG C of sterilizing 20min in high-pressure sterilizing pot, taken out spare into cultivation box after uniformly；
(3) it is inoculated with: after culture medium is cooled to room temperature, moving into aseptic operating platform inoculation as early as possible.Cordyceps militaris strain is used first
Sterile water is diluted to proper density, then accesses the strain of 8%vol into culture medium with liquid spawn inoculator；
(4) bacterium germination: by the media transfer after inoculation to the bacteria-producing room being protected from light, 25 DEG C of temperature are controlled, culture 3d or so, mycelia will
Cover with matrix；
(5) management of producing mushroom: removing bacteria-producing room for the cultivation box of the good mycelia of length, opens light and carries out lighting process, intensity of illumination is
300lx keeps daily illumination 12h, and to the thin out yellow of mycelia to orange-yellow, the sealing cover of opening cultivation box, holding air is opposite
Humidity controls temperature during 16-24 DEG C, flower bud in 85%-95%, guarantees 5 DEG C or so of the temperature difference to stimulate former base to break up；
(6) harvest: when sporophore growth tends to stop, surface starts the shell of ascus for powdery occur, is Harvesting Date；
(7) record data: observation sporophore growth situation records yield data.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (3)
1. a kind of high yield and high quality cordyceps militaris plantation culture medium, which is characterized in that with rice or wheat for main cultivation matrix, addition
The corncob of rice or wheat quality 10%-40%, wheat bran, sawdust or husk, adding mixed liquor ratio is 1:1.1-1:1.5
Culture medium for cultivating is made after sterilizing in the nutrient solution of g/mL；Wherein nutrient solution prescription is calculated by mass percentage are as follows: potassium dihydrogen phosphate
0.15% ~ 0.25%, magnesium sulfate 0.1% ~ 0.2%, calcium carbonate 0.1% ~ 0.18%, vitamin B1 0.0005%-0.0015%, surplus are
Water, the sum of each component are 100%.
2. a kind of high yield and high quality cordyceps militaris plantation method, which is characterized in that the described method comprises the following steps:
(1) compounding: weighing potassium dihydrogen phosphate, magnesium sulfate, calcium carbonate and vitamin B1, is completely dissolved in water and battalion is made
Nutrient solution, nutrient solution prescription are calculated by mass percentage are as follows: potassium dihydrogen phosphate 0.15% ~ 0.25%, magnesium sulfate 0.1% ~ 0.2%, carbonic acid
Calcium 0.1% ~ 0.18%, vitamin B1 0.0005%-0.0015%, surplus are water, and the sum of each component is 100%；
(2) preparation of culture medium: weighing a certain amount of rice or wheat, rice or corncob, the wheat of wheat quality 10-40% is added
Bran, sawdust or husk, then step (1) prepared nutrient solution is added by mixed liquor ratio 1:1.1-1:1.5 g/mL, mixing is equal
Packing will be cultivated box 121 DEG C of sterilizing 20min in high-pressure sterilizing pot, taken out spare into cultivation box after even；
(3) it is inoculated with: after culture medium is cooled to room temperature, moving into aseptic operating platform inoculation as early as possible, first use Cordyceps militaris strain
Sterile water dilution, then the strain of 7-10%vol is accessed into culture medium with liquid spawn inoculator；
(4) bacterium germination: by the media transfer after inoculation to the bacteria-producing room being protected from light, controlling 20-25 DEG C of temperature, cultivates 3d, and mycelia will
Cover with matrix；
(5) management of producing mushroom: removing bacteria-producing room for the cultivation box of the good mycelia of length, opens light and carries out lighting process, intensity of illumination is
300lx keeps daily illumination 10-14h, to the thin out yellow of mycelia to orange-yellow, the sealing cover of opening cultivation box, holding air phase
To humidity in 85%-95%, temperature is controlled during 16-24 DEG C, flower bud, guarantees 5 DEG C of the temperature difference to stimulate former base to break up；
(6) harvest: when sporophore growth tends to stop, surface starts the shell of ascus for powdery occur, is Harvesting Date, and harvesting obtains
High-quality Cordyceps militaris.
3. a kind of high yield and high quality cordyceps militaris plantation method according to claim 2, which is characterized in that in the step (3)
Cordyceps militaris spawn be liquid spawn.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113875496A (en) * | 2021-11-10 | 2022-01-04 | 上海市农业科学院 | Rejuvenation method of cordyceps militaris strains |
| CN115568380A (en) * | 2022-11-08 | 2023-01-06 | 天水众安生物科技有限责任公司 | Crushed wheat culture medium for cultivating cordyceps militaris and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113875496A (en) * | 2021-11-10 | 2022-01-04 | 上海市农业科学院 | Rejuvenation method of cordyceps militaris strains |
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Application publication date: 20190101 |