CN107810781A - A kind of method that silkworm living cultivates Cordyceps militaris - Google Patents

A kind of method that silkworm living cultivates Cordyceps militaris Download PDF

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Publication number
CN107810781A
CN107810781A CN201711102065.7A CN201711102065A CN107810781A CN 107810781 A CN107810781 A CN 107810781A CN 201711102065 A CN201711102065 A CN 201711102065A CN 107810781 A CN107810781 A CN 107810781A
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silkworm
cordyceps militaris
living
inoculation
culture
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黎宇毅
李灵洁
陆世吉
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Guangxi Tian Sha Beach Agricultural Investment Co Ltd
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Guangxi Tian Sha Beach Agricultural Investment Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates

Abstract

The invention discloses a kind of method that silkworm living cultivates Cordyceps militaris, comprise the following steps:(1)The preparation of culture medium;(2)Inoculation, Cordyceps militaris cultural hypha;(3)Silkworm living cultivates Cordyceps militaris;(4)Silkworm living cultivates Cordyceps militaris growing season management;(5)Timely collecting.Present invention silkworm living cultivates needed for the method for Cordyceps militaris that raw material is simple, easily looks for, and planting technology is easy, easily grasps, and strain plantation biological yield is high, and biological transformation ratio is up to more than 90%, and with short production cycle, is adapted to numerous producers' development to plant.

Description

A kind of method that silkworm living cultivates Cordyceps militaris
Technical field
The invention belongs to Cordyceps Militaris production technical field, and in particular to a kind of method that silkworm living cultivates Cordyceps militaris.
Background technology
Cordyceps militaris also known as northern Chinese caterpillar Fungus, Cordceps militaris, are Ascomycotina, ergot Zoopagales, Clavicipitaceae, Cordyceps Type sepecies.Worldwide distribution natural resources quantity is seldom.By stroma (i.e. careless part) and two, sclerotium (i.e. the corpse part of worm) The complex being grouped.Winter larva is lived in seclusion in soil, and mushroom parasitism wherein, draws nutrition, and larva body is interior dead full of mycelia. To summer, seedling is born from larva corpse, likeness in form grass, gathers and obtains before and after the Summer Solstice.The traditional Chinese medical science thinks that cordyceps sinensis enters lung kidney two Through, can tonifying lung it is cloudy, and can kidney-replenishing, cure mainly kidney deficiency, impotence and seminal emission, soreness of waist and knee joint, eak after being ill, chronic cough is weak, phthisical cough phlegm Blood, spontaneous sweating etc., it is a kind of unique Chinese medicine that can balance, adjust negative and positive simultaneously.
Cordyceps militaris Variety Introduction China found first in 1958 in Jilin Province, passed through identification, it is believed that it and cordyceps sinensis It is same category, names as Cordyceps militaris.Main product is in Yunnan (Kunming, peacefulness, river and mountain), Jilin (Antu, Yongji), (Shen, Liaoning Sun), the Inner Mongol (Zhelimu League), be born in pin, broad-leaf forest or mixed forest surface soil layer on the pupal cell of lepidopterous insects.It can adopt Manually cultivated in batches with silkworm and tussah chrysalis, drug effect, pharmacology are similar to wild species even better.Cordyceps militaris than cordyceps sinensis, With it is several incomparable the advantages of:First, Cordyceps militaris is the type sepecies of Cordyceps, widely distributed, by countries in the world, scholar recognizes Know and receive.Second, Cordyceps militaris has been bred as complete stroma under artificial condition.Third, Cordyceps militaris contains cordycepin and cordyceps sinensis Polysaccharide, its unique pharmacological action increasingly cause the height of pharmacy circle to regard.Because Cordyceps militaris has above advantage, turn into cordyceps sinensis Outstanding person in category in medicinal Cordyceps Militaris.
The content of the invention
To solve above-mentioned the shortcomings of the prior art, the present invention provides a kind of method that silkworm living cultivates Cordyceps militaris, had The advantages of technique is simple, with short production cycle, pollution rate is low, biological transformation ratio is up to more than 90%, is adapted to large-scale production.
To achieve these goals, the following technical scheme of present invention offer, a kind of method that silkworm living cultivates Cordyceps militaris, including Following steps:
(1)The preparation of culture medium:Culture medium prescription is by rice 71.17%, corn flour 10%, analysis for soybean powder 10%, dried silkworm chrysalis meal 4%, albumen Peptone 2%, glucose 2%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, vitamin B10.03%th, 0.5% group of the special anti-fouling agent of cordyceps sinensis Into;Culture medium preparation method is:It is 0.5mm to weigh rice, corn flour, analysis for soybean powder to be put into thickness in above culture medium prescription ratio Above, in the polypropylene knuckle polybag or can of 15cm × 25cm sizes, then weigh dried silkworm chrysalis meal and be put into the modeling equipped with rice In pocket or bottle, then peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1, the special anti-fouling agent of cordyceps sinensis by material Water ratio is 1:1.3~1.4 are put into water, after stirring is completely dissolved mixing repeatedly, are configured to the nutrient solution that pH value is 7;Measure and match somebody with somebody The nutrient solution that makes add equipped with rice, dried silkworm chrysalis meal polybag or bottle in, after envelope tightens polybag or bottleneck, immersion prewets 2 ~3h, move into normal-pressure sterilizing pot or high-pressure sterilizing pot and kept for 100 DEG C keep 1~2h sterilizings, bacterium is can access after its cooling Kind;
(2)Inoculation, Cordyceps militaris cultural hypha:Inoculation is put into transfer room with article and inoculation with strain, culture medium, uses aerosol Disinfectant sterilizes 30min;By after Cordyceps militaris is female, original seed appearance carries out cleaning disinfection with 75% alcohol during inoculation, with disinfecting Inoculation hook or tweezers strain is linked into step(1)Culture medium in, each polypropylene knuckle polybag or can inoculation 2 ~3 pieces;Tying after inoculation, moves it into progress dim light culture in 22~25 DEG C of culturing room, and 7~12d of culture covers with polybag Or bottle produces Cordyceps militaris spawn;
(3)Silkworm living cultivates Cordyceps militaris:First silkworm silkworm body living is dissolved in cold water with appropriative disinfector by 2% dosage, stir repeatedly to It is completely dissolved and thimerosal is made, silkworm silkworm body living is put into thimerosal, pulled out after sterilization, after draining moisture, with step(2)Culture Good Cordyceps militaris spawn together moves into cultivating chamber, and inoculation cultivation is carried out after sterilized;During cultivation, take pupa with the instrument disinfected Cordyceps species are spread on the plastic sheeting sterilized, and are covered, and surrounding pressure is tight;
(4)Silkworm living cultivates Cordyceps militaris growing season management:Cultivating chamber illumination keeps 12~14h, and indoor temperature is controlled at 18~22 DEG C, 3~5 DEG C of the temperature difference is controlled round the clock, and humid control is 65~70%;Open a 1cm on a plastic film with blade2Triangle it is small Mouthful, culture 8-11d Chinese caterpillar fungus hyphas all infect silkworm silkworm body living, after silkworm body starts to be hardened, silkworm are chosen and is placed on sterilized place On the plastic sheeting of reason, or moisturizing culture is carried out in polybag or bottle;Initial stage of culture humid control treats fructification 80~85% After growth length exceedes surrounding hyphal surface, relative air humidity is increased to 90~95%;
(5)Timely collecting:After sporophore growth stopping, there is salmon pink sporangium in tip end surface, and fructification is up to 4~8cm When can harvest, during harvesting with the scuppit with elbow by fructification under base portion shovel, the old mycoderma in compost surface is removed after harvesting, Keep the skin wet, then harvest a damp fructification.
Further, the dried silkworm chrysalis meal is to dry or dry the powder worn into after edible silkworm chrysalis or silk cocoon reel off raw silk from cocoons.
Further, the silkworm silkworm body living was 3~4 ages, and length and rugosity are uniformly lived silkworm.
In summary, the present invention has the advantages that as a result of such scheme:
1st, the present invention cultivates cordyceps sinensis with silkworm body and cordyceps species, is only needed 45 days or so from fructification harvesting is inoculated into.At 10 squares The indoor carry out solid tier rack type culture of rice, is respectively cultivated 1 time in spring, autumn, can produce within 1 year the dry cordyceps sinensis of 30~50kg, every kilogram By minimum 2200 yuan sales, removing every kilogram of the expenses such as purchase strain, silkworm can make a profit 1500 yuan, can take in about 40,000~60,000 yuan year, Can smallholder cultivate also plant layoutization cultivate, remarkable in economical benefits.
2nd, raw material is simple, easily looks for needed for the method for present invention silkworm cultivation Cordyceps militaris living, and planting technology is easy, easily grasps, bacterium A variety of plant and biology yield are high, and biological transformation ratio is up to more than 90%, and with short production cycle, are adapted to the numerous producers to develop plantation.
Embodiment
With reference to embodiment, the present invention is described further.
A kind of method that silkworm living cultivates Cordyceps militaris, comprises the following steps:
(1)The preparation of culture medium:Culture medium prescription is by rice 71.17%, corn flour 10%, analysis for soybean powder 10%, dried silkworm chrysalis meal 4%, albumen Peptone 2%, glucose 2%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, vitamin B10.03%th, 0.5% group of the special anti-fouling agent of cordyceps sinensis Into;Culture medium preparation method is:It is 0.5mm to weigh rice, corn flour, analysis for soybean powder to be put into thickness in above culture medium prescription ratio Above, in the polypropylene knuckle polybag or can of 15cm × 25cm sizes, then weigh dried silkworm chrysalis meal and be put into the modeling equipped with rice In pocket or bottle, then peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1, the special anti-fouling agent of cordyceps sinensis by material Water ratio is 1:1.3~1.4 are put into water, after stirring is completely dissolved mixing repeatedly, are configured to the nutrient solution that pH value is 7;Measure and match somebody with somebody The nutrient solution that makes add equipped with rice, dried silkworm chrysalis meal polybag or bottle in, after envelope tightens polybag or bottleneck, immersion prewets 2 ~3h, move into normal-pressure sterilizing pot or high-pressure sterilizing pot and kept for 100 DEG C keep 1~2h sterilizings, bacterium is can access after its cooling Kind;
(2)Inoculation, Cordyceps militaris cultural hypha:Inoculation is put into transfer room with article and inoculation with strain, culture medium, uses aerosol Disinfectant sterilizes 30min;By after Cordyceps militaris is female, original seed appearance carries out cleaning disinfection with 75% alcohol during inoculation, with disinfecting Inoculation hook or tweezers strain is linked into step(1)Culture medium in, each polypropylene knuckle polybag or can inoculation 2 ~3 pieces;Tying after inoculation, moves it into progress dim light culture in 22~25 DEG C of culturing room, and 7~12d of culture covers with polybag Or bottle produces Cordyceps militaris spawn;
(3)Silkworm living cultivates Cordyceps militaris:First silkworm silkworm body living is dissolved in cold water with appropriative disinfector by 2% dosage, stir repeatedly to It is completely dissolved and thimerosal is made, silkworm silkworm body living is put into thimerosal, pulled out after sterilization, after draining moisture, with step(2)Culture Good Cordyceps militaris spawn together moves into cultivating chamber, and inoculation cultivation is carried out after sterilized;During cultivation, take pupa with the instrument disinfected Cordyceps species are spread on the plastic sheeting sterilized, and are covered, and surrounding pressure is tight;
(4)Silkworm living cultivates Cordyceps militaris growing season management:Cultivating chamber illumination keeps 12~14h, and indoor temperature is controlled at 18~22 DEG C, 3~5 DEG C of the temperature difference is controlled round the clock, and humid control is 65~70%;Open a 1cm on a plastic film with blade2Triangle it is small Mouthful, culture 8-11d Chinese caterpillar fungus hyphas all infect silkworm silkworm body living, after silkworm body starts to be hardened, silkworm are chosen and is placed on sterilized place On the plastic sheeting of reason, or moisturizing culture is carried out in polybag or bottle;Initial stage of culture humid control treats fructification 80~85% After growth length exceedes surrounding hyphal surface, relative air humidity is increased to 90~95%;
(5)Timely collecting:After sporophore growth stopping, there is salmon pink sporangium in tip end surface, and fructification is up to 4~8cm When can harvest, during harvesting with the scuppit with elbow by fructification under base portion shovel, the old mycoderma in compost surface is removed after harvesting, Keep the skin wet, then harvest a damp fructification.
Wherein, dried silkworm chrysalis meal is to dry or dry the powder worn into after edible silkworm chrysalis or silk cocoon reel off raw silk from cocoons;
Silkworm silkworm body living be 3~4 ages, the uniform silkworm living of length and rugosity.
Use the present embodiment from fructification collecting time is inoculated into for 45 days.In 10 square metres of indoor carry out solid layer frame Formula culture, respectively cultivated in spring, autumn 1 time, the dry cordyceps sinensis of 50kg can be produced within 1 year, every kilogram, by minimum 2200 yuan sales, removes purchase Every kilogram of the expenses such as strain, silkworm can make a profit 1500 yuan, can take in about 60,000 yuan year.
The preferred embodiment of invention is the foregoing is only, is not intended to limit the invention, all spirit in the present invention Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.

Claims (3)

1. a kind of method that silkworm living cultivates Cordyceps militaris, it is characterised in that the technique comprises the following steps:
(1)The preparation of culture medium:Culture medium prescription is by rice 71.17%, corn flour 10%, analysis for soybean powder 10%, dried silkworm chrysalis meal 4%, albumen Peptone 2%, glucose 2%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, vitamin B10.03%th, 0.5% group of the special anti-fouling agent of cordyceps sinensis Into;Culture medium preparation method is:It is 0.5mm to weigh rice, corn flour, analysis for soybean powder to be put into thickness in above culture medium prescription ratio Above, in the polypropylene knuckle polybag or can of 15cm × 25cm sizes, then weigh dried silkworm chrysalis meal and be put into the modeling equipped with rice In pocket or bottle, then peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1, the special anti-fouling agent of cordyceps sinensis by material Water ratio is 1:1.3~1.4 are put into water, after stirring is completely dissolved mixing repeatedly, are configured to the nutrient solution that pH value is 7;Measure and match somebody with somebody The nutrient solution that makes add equipped with rice, dried silkworm chrysalis meal polybag or bottle in, after envelope tightens polybag or bottleneck, immersion prewets 2 ~3h, move into normal-pressure sterilizing pot or high-pressure sterilizing pot and kept for 100 DEG C keep 1~2h sterilizings, bacterium is can access after its cooling Kind;
(2)Inoculation, Cordyceps militaris cultural hypha:Inoculation is put into transfer room with article and inoculation with strain, culture medium, uses aerosol Disinfectant sterilizes 30min;By after Cordyceps militaris is female, original seed appearance carries out cleaning disinfection with 75% alcohol during inoculation, with disinfecting Inoculation hook or tweezers strain is linked into step(1)Culture medium in, each polypropylene knuckle polybag or can inoculation 2 ~3 pieces;Tying after inoculation, moves it into progress dim light culture in 22~25 DEG C of culturing room, and 7~12d of culture covers with polybag Or bottle produces Cordyceps militaris spawn;
(3)Silkworm living cultivates Cordyceps militaris:First silkworm silkworm body living is dissolved in cold water with appropriative disinfector by 2% dosage, stir repeatedly to It is completely dissolved and thimerosal is made, silkworm silkworm body living is put into thimerosal, pulled out after sterilization, after draining moisture, with step(2)Culture Good Cordyceps militaris spawn together moves into cultivating chamber, and inoculation cultivation is carried out after sterilized;During cultivation, take pupa with the instrument disinfected Cordyceps species are spread on the plastic sheeting sterilized, and are covered, and surrounding pressure is tight;
(4)Silkworm living cultivates Cordyceps militaris growing season management:Cultivating chamber illumination keeps 12~14h, and indoor temperature is controlled at 18~22 DEG C, 3~5 DEG C of the temperature difference is controlled round the clock, and humid control is 65~70%;Open a 1cm on a plastic film with blade2Triangle it is small Mouthful, culture 8-11d Chinese caterpillar fungus hyphas all infect silkworm silkworm body living, after silkworm body starts to be hardened, silkworm are chosen and is placed on sterilized place On the plastic sheeting of reason, or moisturizing culture is carried out in polybag or bottle;Initial stage of culture humid control treats fructification 80~85% After growth length exceedes surrounding hyphal surface, relative air humidity is increased to 90~95%;
(5)Timely collecting:After sporophore growth stopping, there is salmon pink sporangium in tip end surface, and fructification is up to 4~8cm When can harvest, during harvesting with the scuppit with elbow by fructification under base portion shovel, the old mycoderma in compost surface is removed after harvesting, Keep the skin wet, then harvest a damp fructification.
2. the method that a kind of silkworm living according to claim 1 cultivates Cordyceps militaris, it is characterised in that:The dried silkworm chrysalis meal is edible Silkworm chrysalis or silk cocoon dry or dried the powder worn into after reeling off raw silk from cocoons.
3. the method that a kind of silkworm living according to claim 1 cultivates Cordyceps militaris, it is characterised in that:The silkworm silkworm body living is 3 ~4 ages, length and rugosity uniformly live silkworm.
CN201711102065.7A 2017-11-10 2017-11-10 A kind of method that silkworm living cultivates Cordyceps militaris Pending CN107810781A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108633615A (en) * 2018-04-24 2018-10-12 王道富 A method of cultivating cordyceps sinensis with silkworm larvae and pupa worm
CN109105148A (en) * 2018-08-21 2019-01-01 福建农林大学 A kind of high yield and high quality cordyceps militaris plantation culture medium and its cultural method
CN114793755A (en) * 2022-04-18 2022-07-29 常熟理工学院 Method for cultivating silkworm cordyceps militaris with high rigidification rate
CN115553179A (en) * 2022-10-08 2023-01-03 濮阳市天元虫草研发有限公司 Artificial cordyceps militaris and preparation method thereof

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108633615A (en) * 2018-04-24 2018-10-12 王道富 A method of cultivating cordyceps sinensis with silkworm larvae and pupa worm
CN109105148A (en) * 2018-08-21 2019-01-01 福建农林大学 A kind of high yield and high quality cordyceps militaris plantation culture medium and its cultural method
CN114793755A (en) * 2022-04-18 2022-07-29 常熟理工学院 Method for cultivating silkworm cordyceps militaris with high rigidification rate
CN115553179A (en) * 2022-10-08 2023-01-03 濮阳市天元虫草研发有限公司 Artificial cordyceps militaris and preparation method thereof

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Application publication date: 20180320