CN1091772A - The method of using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus - Google Patents
The method of using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus Download PDFInfo
- Publication number
- CN1091772A CN1091772A CN 93102809 CN93102809A CN1091772A CN 1091772 A CN1091772 A CN 1091772A CN 93102809 CN93102809 CN 93102809 CN 93102809 A CN93102809 A CN 93102809A CN 1091772 A CN1091772 A CN 1091772A
- Authority
- CN
- China
- Prior art keywords
- silkworm chrysalis
- baked
- chinese caterpillar
- caterpillar fungus
- baked silkworm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Mushroom Cultivation (AREA)
Abstract
The present invention relates to a kind of cultural method of silkworm chrysalis Cordyceps sinensis.This method be with inoculation on slant medium, carry out the cultivation in mycelia stage; Using baked silkworm chrysalis is clean and dry with flushing with clean water; The processing of on using baked silkworm chrysalis, punchinging; With cleaning the using baked silkworm chrysalis bottling of punchinging, add suitable quantity of water, carry out autoclave sterilization; A little mycelia piece of picking is put on the pupal cell of bottle in inoculation tank, the envelope flask culture, and temperature is 15 ℃-30 ℃, natural lighting can grow Cordyceps sporophore in about two months.Using baked silkworm chrysalis helps a large amount of purchases and long-term storage, and the using baked silkworm chrysalis after punchinging can improve the success ratio of artificially breeding Chinese caterpillar fungus through autoclave sterilization.
Description
The invention belongs to fungi and cultivate the field, particularly relate to a kind of cultural method of silkworm chrysalis Cordyceps sinensis.
Cordyceps militaris (L.) Link. is a kind of famous biological medicine, resource scarcity.The method of a kind of artificial culture Cordyceps militaris (L.) Link. has been introduced by Patent Office of the People's Republic of China on August 2nd, 89 disclosed " artificial culturing method of silkworm chrysalis Cordyceps sinensis " (publication number CN1034390A), and this cultural method is to carry out on the bright pupa of tussah, and certain limitation is arranged.The pupa of living is adopted the body surface sterilization, can only kill the assorted bacterium of body surface, can not destroy pupa skin wax, inoculation postoperative infection rate is lower.
The objective of the invention is to overcome the deficiency of prior art, propose the method that a kind of using baked silkworm chrysalis is cultivated Chinese caterpillar fungus.
Technical scheme of the present invention is such.Gather wild cordyceps militaris, separate and purifying, ready-made bacterial strain is carried out seed selection and rejuvenation; Inoculation on slant medium, is carried out the cultivation in mycelia stage; Using baked silkworm chrysalis is clean and dry with flushing with clean water; The processing of on using baked silkworm chrysalis, punchinging; With cleaning the using baked silkworm chrysalis bottling of punchinging, add suitable quantity of water, carry out autoclave sterilization; In inoculation tank, a little mycelia piece of picking is put on the pupal cell of bottle, the envelope flask culture, and temperature is 15 ℃-30 ℃, natural lighting promptly grew Cordyceps sporophore in about two months.
The present invention has following advantage:
1, the raw material of silkworm chrysalis adopts oven dry to handle, and helps a large amount of purchases, and long-term storage and quantity-produced purpose in batch are for suitability for industrialized production provides favourable guarantee.
2, the using baked silkworm chrysalis after punchinging adopts the autoclave sterilization method, can improve the inoculation infection rate, has improved the success ratio of artificially breeding Chinese caterpillar fungus.
Embodiments of the invention are as follows:
1, gathers Cordyceps militaris spawn, wild strain is separated and purifying.Ready-made bacterial strain is carried out seed selection and rejuvenation.One-level kind, secondary kind and three grades of kinds will be made behind the protospecies breeding.No matter be that original seed or one-level kind, secondary kind, three grades of kinds can be directly used in inoculation, but normally original seed or one-level kind, secondary kind be used for preserving, Cordyceps militaris (L.) Link. are cultivated in three grades of kinds inoculations.
2, with bacterial classification inoculation to slant medium.The cooperation and the making processes of slant medium are: take by weighing peeling potato 200 grams, glucose 20 grams, agar 20 grams, potassium primary phosphate 1 gram, put into 1000 milliliters beaker, adding suitable quantity of water and heated and stirred dissolves it, stop heating after waiting to dissolve, and fill it up with 1000 milliliters of warm water, back packing test tube stirs, jump a queue, put into the inclined-plane, cooling, i.e. bevel substratum.Slant medium promptly can be used for cultivating and expanding numerous bacterial classification behind autoclave sterilization.Be used to inoculate the bacterial classification of cultivating Cordyceps militaris (L.) Link., at first carry out the cultivation in mycelia stage, promptly under 23-27 ℃ temperature and natural lighting, cultivated about 20 days, mycelia is reached full growth, with infectivity that increases bacterial classification and the cultivation time that shortens Cordyceps militaris (L.) Link..
3, silk cocoon was dried through 12 hours under 65 ℃ temperature, after the complete dehydration of pupa becomes dry, use plastic bag packaging seal, so that long-term storage and standby.The silkworm chrysalis (cutting off the cocoon skin) of preparing inoculation is clean and dry with flushing with clean water.
4, on the pupa skin, punching with suitable sharp device many places such as mallets.As moisture content is entered, pupal cell is interior or Cordyceps is infected to body by body surface like this.
5, using baked silkworm chrysalis is packed in the clean Cans, whenever bottledly go into about 10, be not advisable, add suitable quantity of water (equate with bright pupa water content or summary many), and seal a bottle, carry out autoclave sterilization and (put into pressure kettle, at 1.2 kilograms/CM so that the bottle end of swing is completely overlapping
2Pressure down keep get final product half an hour.Cans after the sterilization are taken out, after cooling, move into the aseptic processing room.
6, on aseptic processing room and Bechtop (or in inoculation tank), the Cans of dress silkworm chrysalis are sealed and open, above the flame of spirit lamp, with a little mycelia piece of inoculator picking, moved in the Cans by test tube, seal up bottleneck (tightening with kraft paper, plastic film and rope) and be placed on the stand, room temperature is controlled between 15-30 ℃, natural lighting is cultivated down, and about about two months promptly grows Cordyccps-militaris-(L.)-link. Sporophore.
The forming process of sporophore is: the inoculation mycelia infect silkworm chrysalis body surface (body surface is Bai Mao and progressively diffusion) → by the body surface passing hole channel invade gradually in the silkworm chrysalis body → in-vivo tissue is stiff gradually after infecting, become the same shape of grass, be sporophore → no longer grow and atrophy occurs when sporophore, be considered as maturation, before can gathering → gathering, the bottle closure that opens a tin allows it dry naturally or air-dry, and the plastic bag sealing of packing into of then Cordyceps militaris (L.) Link. being gathered is preserved.
Claims (3)
1, the method of using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus, method is that Cordyceps militaris (L.) Link. fungus is inoculated on the substratum, cultivating about 20 days under 23-27 ℃ temperature and the natural lighting, again cultured mycelia is put on the pupa that disinfects, continue then to cultivate, it is characterized in that the silkworm chrysalis that adopts is a using baked silkworm chrysalis, using baked silkworm chrysalis needs to punching on the pupa skin with sharp device before sterilization, silkworm chrysalis packed into add an amount of water in the bottle and carry out autoclave sterilization, (or on Bechtop) inoculated in inoculation tank, temperature (was being cultivated 50-70 days under the natural lighting between 5-30 ℃, can grown Cordyccps-militaris-(L.)-link. Sporophore.
2, the method for using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus according to claim 1 is characterized in that said using baked silkworm chrysalis is that silk cocoon was dried through 12 hours under 65 ℃ temperature.
3, the method for using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus according to claim 1, it is characterized in that inoculating is that the sealing of Cans that will adorn silkworm chrysalis earlier opened, above the flame of spirit lamp, with a little mycelia piece of inoculator picking, move into by test tube in the bottle of dress silkworm chrysalis, seal up bottleneck.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93102809 CN1091772A (en) | 1993-03-06 | 1993-03-06 | The method of using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93102809 CN1091772A (en) | 1993-03-06 | 1993-03-06 | The method of using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1091772A true CN1091772A (en) | 1994-09-07 |
Family
ID=4984315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93102809 Pending CN1091772A (en) | 1993-03-06 | 1993-03-06 | The method of using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1091772A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627701B (en) * | 2009-08-14 | 2010-12-08 | 郑杰辉 | Method for breeding cordyceps militars strain |
| CN103749149A (en) * | 2013-12-20 | 2014-04-30 | 凌中鑫 | Method for cultivating artificial worm grass with mealworm pupa as carrier |
| CN105103948A (en) * | 2015-08-21 | 2015-12-02 | 李其智 | Cultivating method for cordyceps militaris |
| CN107810781A (en) * | 2017-11-10 | 2018-03-20 | 广西沙田仙人滩农业投资有限公司 | A kind of method that silkworm living cultivates Cordyceps militaris |
| CN112190602A (en) * | 2020-10-27 | 2021-01-08 | 苏州家和蚕业生物科技有限公司 | Preparation method of silkworm chrysalis cordyceps sinensis tablets |
-
1993
- 1993-03-06 CN CN 93102809 patent/CN1091772A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627701B (en) * | 2009-08-14 | 2010-12-08 | 郑杰辉 | Method for breeding cordyceps militars strain |
| CN103749149A (en) * | 2013-12-20 | 2014-04-30 | 凌中鑫 | Method for cultivating artificial worm grass with mealworm pupa as carrier |
| CN105103948A (en) * | 2015-08-21 | 2015-12-02 | 李其智 | Cultivating method for cordyceps militaris |
| CN107810781A (en) * | 2017-11-10 | 2018-03-20 | 广西沙田仙人滩农业投资有限公司 | A kind of method that silkworm living cultivates Cordyceps militaris |
| CN112190602A (en) * | 2020-10-27 | 2021-01-08 | 苏州家和蚕业生物科技有限公司 | Preparation method of silkworm chrysalis cordyceps sinensis tablets |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102273378B (en) | Bottle cultivation method for Hypsizigus marmoreus | |
| CN104541938B (en) | A kind of ganoderma lucidum merges cultural method | |
| CN101720627A (en) | Method for culturing cordyceps militaris by living silkworm chrysalises | |
| CN103270887B (en) | Silkworm chrysalis northern Chinese caterpillar Fungus industrial cultivation technique | |
| CN106818207B (en) | A kind of bag cultivation growing straight method of needle mushroom | |
| CN106613355A (en) | Ecological planting method of oyster mushrooms | |
| CN109220545A (en) | Using pine cedar sawdust as the Sparassis crispa cultivation matrix of raw material and its Sparassis crispa cultural method | |
| CN110367039A (en) | A kind of production method reducing Neurospora infection Dictyophora rubrovalvata cultivar | |
| KR100823541B1 (en) | Mushroom cultivation method | |
| CN107125028A (en) | A kind of wild yellow ring squama agaric domestication and artificial culturing method | |
| CN105009940A (en) | Method for culturing enoki mushroom with herb residues | |
| CN106856984A (en) | A kind of Hydnum tree and its cultural method | |
| CN101743846B (en) | Method for shortening culture cycle of bag-cultivation lyophyllum decastes | |
| CN106613336A (en) | Lucid ganoderma planting method | |
| CN1091772A (en) | The method of using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus | |
| CN1043848A (en) | Golden fungus substituting stuff cultivation method | |
| CN107711285A (en) | A kind of method of culture bottle factory culture Hericium erinaceus | |
| CN107216178A (en) | A kind of true pleurotus cornucopiae cultural method | |
| JPH07184473A (en) | Method for artificially cultivating mushroom of genus pleurotus | |
| CN112442449A (en) | Ramaria bacteria stock culture medium and application thereof, and Ramaria bacteria stock and culture method thereof | |
| KR20200012367A (en) | How to grow flower mushrooms | |
| JP2000287537A (en) | Culture of spawn of volvariella speciosa sing. var. volvacea and production of mushroom bed for cultivating volvariella speciosa sing. var. volvacea | |
| CN106754402A (en) | Lucidum strain preparation method | |
| CN1080464A (en) | Simple edible mashroom cultivating method and mother culture media | |
| CN106834132A (en) | Ganoderma lucidum mycelium preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |