CN1043848A - Golden fungus substituting stuff cultivation method - Google Patents
Golden fungus substituting stuff cultivation method Download PDFInfo
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- CN1043848A CN1043848A CN 89100117 CN89100117A CN1043848A CN 1043848 A CN1043848 A CN 1043848A CN 89100117 CN89100117 CN 89100117 CN 89100117 A CN89100117 A CN 89100117A CN 1043848 A CN1043848 A CN 1043848A
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Abstract
The invention belongs to the fungus growing technique field, its main contents are the breeding method of mother culture of tremella in the golden fungus substituting stuff cultivation, original seed, cultivated species and composts or fertilisers of cultivating, kind and the prescription of substituting stuff cultivation gold ear; Cultivation and management method.The present invention cultivates golden ear can save timber, and the cycle is short, the productive rate height, and cultivation is not subject to seasonal restrictions, and is suitable for applying.
Description
The invention belongs to the fungus growing technique field.
The gold ear claims yellow fungus again, belongs to Basidiomycetes, Tremellales, Tremellaceae, Tremella (Tremella).Former formal name used at school is Tremella auranria Schw.ex Fr, now orders Tremella aurantialba Bandoni et Zang by name.The gold ear is distinctive a kind of famous edible mushroom in China southwest and medicinal fungus.Its culture technique is one of China's edible mushroom scientific research project, and the artificial wood section cultivation has succeeded () in recent years, but substituting stuff cultivation gold ear is not succeeded.
The gold ear is a kind of parasitic edible mushroom, when natural world or artificial wood section cultivation, the gold ear is by being provided nutriment, lead fungi again by decomposing its saprophytic yellow mao Qinggang [Cyclobalanopsis delavayi(Franch) Schottky] by several lead fungi mycelia such as parasitic flat tough lead fungi [Stereum fasciatum Schw], hair Boreostereum vibrans [Stereum hirsutum(willd) Fr], trees such as Qinggang [Cyclobalanopsis Glanca(Thunb) Oersted] and obtain nutriment.Be made up of tremella filament and lead fungi mycelia by dissecting the golden ears or side handles of a utensil entity of discovery, it is the tremella silk that golden ears or side handles of a utensil entity skin is flavous.
Substituting stuff cultivation gold ear mainly has problems and is at present: (1) fruit body is changeed golden yellow difficulty by white; (2) fruiting body yield is low; (3) it is low to go out the ear rate; (4) fruit body easy infection Trichoderma viride, mould and rotting.
The objective of the invention is to solve the problem of above existence, seek a kind of from bacterial classification production, medium proportioning, the golden fungus substituting stuff cultivation method of complete sets of Techniques such as cultivation management.
Particular content of the present invention is as follows:
At first bacterial classification production characteristics are directly to produce golden ears or side handles of a utensil entity bacterial classification, and its female kind, original seed cultivated species are all used fruit body, and the bacterium ball that just forms with the tremella silk when having only the liquid fermentation production of hybrid seeds is made bacterial classification.Compare with existing segment wood cultivated tremella kind production method, bacterial classification of the present invention is produced in the medium and has been added corn flour (grain), has improved golden ears or side handles of a utensil entity output.Table (1) is medium and the bacterial classification production medium of segment wood cultivated conventional use and the comparison of distinct methods results fruiting body yield that the present invention uses.The present invention produces the third method of bacterial classification by (3) prescription in the table (1), makes liquid nutrient medium, and parallel operation is shaken in use or fermentation tank carries out the submerged fermentation cultivation.Shake parallel operation can use reciprocating type, hunting speed 80-130 time/minute.
The golden fungus substituting stuff cultivation substitute can be with various broad-leaved tree sawdust and cotton seed hulls.From yellow hair Qinggang, Qinggang, cork (Quercus Variabilis BL) multiple ear lithocarpus (Lithocarepus polyotachus A.Camus) Castanopsis delavayi Franch (Castanopsis delavayi Franch), Quercus acutissima (Quercus acutissima Carr) toothed oak (Quercur dentata Thunb Var.Oxyloba Franch), Anlnus nepalensis Fagaceaes (Fagaceae) such as (Alnus nepalensis D.Don) and Betulaceae (Betulaceae) trees sawdust are better in the broad-leaved tree.
Cultivating container useable glass Cans, 500-2000 ml polypropylene plastic bottle (the shoulder upper part can take off), polypropylene or low-pressure polyethylene plastic sack.
The cultivation prescription adopts broad-leaved tree sawdust (or cotton seed hulls) 60-80%, bran 5-25%, and corn flour 5-40%, potassium dihydrogen phosphate 0-0.2%, gypsum 0-5%, water is an amount of.
Cultivation method, by the even composts or fertilisers of cultivating of above-mentioned prescription mix, bottling or pack are after insert the golden ears or side handles of a utensil entity bacterial classification of broad bean size behind the autoclaving, cover bacterial classification with a small amount of composts or fertilisers of cultivating, the culturing room that puts into 10-25 ℃ cultivated 20-50 days, to there be the bottle (bag) of golden ears or side handles of a utensil entity growth and the bottle (bag) of no golden ears or side handles of a utensil entity growth to separate, the bottle (bag) that does not have a growth of golden ears or side handles of a utensil entity is sent into inoculating hood and make a call to a duck eye on medium, again reseed golden ears or side handles of a utensil entity, cover golden ears or side handles of a utensil entity bacterial classification with the medium controlled out again, improve and the ear rate.When the bottle (bag) of existing fruit body is put into the fruiting room and is treated that fruit body is long and arrive diameter 3-5 centimetre, open bottle (bag) covering or take down plastic bottle on the shoulders with upper part, allow each part of golden ears or side handles of a utensil entity expose in the air of circulation, keep relative air humidity at 80-95%, light intensity is at the 20-500 lux, the gold ear transfers golden yellow to, can gather in the crops when fruit body is no longer grown up, cut off fruit body with knife during results, stay base portion, be incubated, preserve moisture, impel second batch of fruit body to grow.Fruiting phase mushroom room temperature is lower than 20 ℃ can reduce the Trichoderma viride infection.Can not directly on golden ears or side handles of a utensil entity, spray during water spray, otherwise cause mashed ear.Take place to spray benzimidazole germicides such as carbendazim, topsin, thiophanate methyl when Trichoderma viride infects more, add these bactericide in the time of also can preparing composts or fertilisers of cultivating.
Below be embodiments of the invention:
Embodiment 1. cultivates (1) according to bacterial classification production of the present invention and takes by weighing corn flour 100 grams, albumen 1 gram, agar 20 grams, 1000 milliliters in water, heating is dissolved agar, the corn flour gelatinization, be divided in the conical flask then, tampon is put into high-pressure sterilizing pot and was sterilized 60 minutes down through 1 kg/cm steam pressure beyond the Great Wall, puts into inoculating hood after the cooling, insert the golden ears or side handles of a utensil entity of iblet size, 22 ℃ constant incubator put into temperature and is by tampon beyond the Great Wall, cultivates 24 hours lead fungi on the golden ears or side handles of a utensil entity and stretches out mycelia, the lead fungi mycelia was covered with media surface in 5-8 days, the golden ears or side handles of a utensil entity that gold ears or side handles of a utensil entity begins to grow or the next door blastogenesis of bacterial classification piece makes new advances, golden ears or side handles of a utensil entity is long to fresh weight 20-50.6 gram after 30-40 days, and has and partly transfer golden yellow to.
Golden ear cultivation method provided by the present invention is compared with Duan Mufa, not only can save timber, and the cycle is short, the biological yield height, and cultivation is not subject to seasonal restrictions.
Embodiment 2. is by yellow hair Qinggang sawdust 75%, corn flour 10%, wheat bran 15%, in the 500 milliliters of seed bottle of packing into after an amount of mix of water is even, pocket is to bottle volume 2/3rds places, and the dark 2 centimetres hole that strikes with a stick, tampon beyond the Great Wall, put into high-pressure sterilizing pot and sterilized 90 minutes under 1 kg/cm steam pressure, put into the inoculating hood inoculation after the cooling, inoculation bacterial classification piece is advisable with the broad bean size.Put into culturing room after the inoculation and cultivate the outstanding charge level of golden ears or side handles of a utensil entity after month, every bottle of siccative 130 grams can grow 50-70 and restrain golden ears or side handles of a utensil entity behind the one and a half months, can make bacterial classification and use.
Embodiment 3. presses cotton seed hulls 75%, and corn flour 15%, wheat bran 10%, water are packed in the plastic sack of 17 centimetres of diameters in right amount, and one of plastic sack is tightened with cotton thread, and the other end puts neck ring and tampon, and composts or fertilisers of cultivating can only pack 3/5ths of sub-volume in the plastic sack.Charge level is stamped three holes that the hole is dark 2 centimetres.Send into high-pressure sterilizing pot, sterilization is 2.5 hours under 1 kg/cm steam pressure, three of the golden ears or side handles of a utensil entity bacterial classifications of broad bean size are inserted in the cooling back in inoculating hood, sending into culturing room cultivates when treating golden ears or side handles of a utensil entity length to 3-5 centimetre after 1.5-2 month, take off neck ring, open plastic sack, keep culturing room's temperature 10-20 ℃ of scope, relative moisture is at 80-90%, luminous intensity is the 20-500 lux, cultivate and no longer grew up when golden ears or side handles of a utensil entity in 8-15 days, and change into to golden yellow and can gather in the crops.
Claims (5)
1, a kind of golden fungus substituting stuff cultivation method belongs to the fungus growing technique field, has comprised that the tremella kind produces the inoculation of substituting stuff cultivation and cultivate material or change generation directly with golden ears or side handles of a utensil entity, it is characterized in that:
A, tremella kind medium adopt the medium that contains corn flour (grain), and cultivation generation material adopts and contains the cultivation of corn flour (grain) and broad-leaved tree sawdust or cotton seed hulls for material.
B, adopted the bottle bag that does not go out golden ears or side handles of a utensil entity after the golden ear inoculated and cultured has partly been mended the operation that connects golden ears or side handles of a utensil entity.
C, adopted golden ear annesl operation.
D, adopted the minimizing hurdle ear operation of spraying medicines such as carbendazim.
2,, it is characterized in that the above-mentioned medium that contains corn flour (grain) is divided into three kinds of tremella kind medium compositions according to the described golden fungus substituting stuff cultivation method of claim 1:
(1), 10% corn flour, 0.1% peptone (or yeast extract), 2% agar, 1000 ml waters.
(2), 60～80% broad-leaved tree sawdust (or cotton seed hulls), 0～25% wheat bran, 5～40% corn flour (grain), 0～0.2% potassium dihydrogen phosphate, 0～0.1% peptone (or yeast extract).
(3), 10% corn flour, 0.1% peptone (or yeast soaks), 1000 ml waters.
3,, it is characterized in that above-mentioned golden ear cultivation generation material adopts the cultivation that contains corn flour (grain) and broad-leaved tree sawdust or cotton seed hulls to be meant for material according to the described Jin Erdaimi cultivation method of claim 1:
(1), the broad-leaved tree sawdust in this cultivation generation material is better with Fagaceaes such as yellow hair Qinggang, Qinggang, cork oak, multiple ear lithocarpus, Castanopsis delavayi Franch, Quercus acutissima, toothed oak tree, Anlnus nepalensis, birch water section seeds sawdust.
(2), the prescription of this cultivation generation material is: 60～80% broad-leaved tree sawdust (or cotton seed hulls), 0～25% wheat bran, 5～40% corn flour (grain), 0～0.2% potassium dihydrogen phosphate, 0-0.1% peptone (or yeast extract), an amount of water.
4, golden fungus substituting stuff cultivation method according to claim 1, it is characterized in that above-mentioned golden ear annesl operation is meant, when golden ears or side handles of a utensil entity length arrives diameter 3-5 centimetre, open bottle, each partly exposes in air to make golden ears or side handles of a utensil entity, keeps 0-20 ℃ of air themperature, relative moisture 80-95%, intensity of illumination 20-500 lux simultaneously waters and can not directly water on the fruit body when golden ear annesl, treats that golden ears or side handles of a utensil entity transfers golden yellow to and begins to gather before discharging spore.
5, golden ears or side handles of a utensil entity cultivation method according to claim 1, it is characterized in that the minimizing of medicines such as above-mentioned sprinkling carbendazim, mashed ear operation is meant, add or golden ears or side handles of a utensil entity colour-change period in golden ear cultivate material, spray benzimidazole bactericide such as carbendazim, topsin or thiophanate methyl and take place to reduce mashed ear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 89100117 CN1043848A (en) | 1989-01-05 | 1989-01-05 | Golden fungus substituting stuff cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 89100117 CN1043848A (en) | 1989-01-05 | 1989-01-05 | Golden fungus substituting stuff cultivation method |
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| CN1043848A true CN1043848A (en) | 1990-07-18 |
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| CN 89100117 Pending CN1043848A (en) | 1989-01-05 | 1989-01-05 | Golden fungus substituting stuff cultivation method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050673A (en) * | 2010-11-11 | 2011-05-11 | 刘晋才 | Preparation method of edible mushroom wood plant mother culture medium and product thereof |
| CN103396198A (en) * | 2013-08-22 | 2013-11-20 | 申洁 | Tremella aurantialba culture medium and culture preparation method |
| CN104892263A (en) * | 2015-06-19 | 2015-09-09 | 桂林健成生物科技开发有限公司 | Application of siraitia grosvenorii fruit residues in cultivation of tremella aurantialba |
| CN105340570A (en) * | 2015-11-03 | 2016-02-24 | 韶关市玉蕈菌业有限公司 | Tremella aurantia industrial cultivation method |
| CN106613349A (en) * | 2016-12-15 | 2017-05-10 | 普洱滇洪俊生物科技开发有限公司 | Tremella aurantialba substitute cultivation method |
| CN107125018A (en) * | 2017-03-28 | 2017-09-05 | 云南顶兴农业科技发展有限公司 | A kind of packed breeding method of golden ear |
| CN107434705A (en) * | 2016-05-26 | 2017-12-05 | 桂林洁宇环保科技有限责任公司 | The golden ear high-yield culturing material made with multiple kinds of crops discarded object |
| CN108377844A (en) * | 2018-05-30 | 2018-08-10 | 武汉环绿时代生态园林工程有限公司 | A kind of cultural method of black fungus |
| CN111357564A (en) * | 2020-04-01 | 2020-07-03 | 云南菌视界生物科技有限公司 | Tremella aurantialba sporocarp fusion symbiotic culture method |
| CN111990164A (en) * | 2020-09-21 | 2020-11-27 | 中华全国供销合作总社昆明食用菌研究所 | Tremella aurantialba strain No. 1 in new Tremella aurantialba strains and cultivation method thereof |
| CN112021070A (en) * | 2020-09-11 | 2020-12-04 | 云南菌视界生物科技有限公司 | Industrialized three-stage cultivation method of tremella aurantialba |
-
1989
- 1989-01-05 CN CN 89100117 patent/CN1043848A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050673A (en) * | 2010-11-11 | 2011-05-11 | 刘晋才 | Preparation method of edible mushroom wood plant mother culture medium and product thereof |
| CN102050673B (en) * | 2010-11-11 | 2013-06-19 | 刘晋才 | Preparation method of edible mushroom wood plant mother culture medium and product thereof |
| CN103396198A (en) * | 2013-08-22 | 2013-11-20 | 申洁 | Tremella aurantialba culture medium and culture preparation method |
| CN104892263A (en) * | 2015-06-19 | 2015-09-09 | 桂林健成生物科技开发有限公司 | Application of siraitia grosvenorii fruit residues in cultivation of tremella aurantialba |
| CN105340570A (en) * | 2015-11-03 | 2016-02-24 | 韶关市玉蕈菌业有限公司 | Tremella aurantia industrial cultivation method |
| CN107434705A (en) * | 2016-05-26 | 2017-12-05 | 桂林洁宇环保科技有限责任公司 | The golden ear high-yield culturing material made with multiple kinds of crops discarded object |
| CN106613349A (en) * | 2016-12-15 | 2017-05-10 | 普洱滇洪俊生物科技开发有限公司 | Tremella aurantialba substitute cultivation method |
| CN107125018A (en) * | 2017-03-28 | 2017-09-05 | 云南顶兴农业科技发展有限公司 | A kind of packed breeding method of golden ear |
| CN108377844A (en) * | 2018-05-30 | 2018-08-10 | 武汉环绿时代生态园林工程有限公司 | A kind of cultural method of black fungus |
| CN111357564A (en) * | 2020-04-01 | 2020-07-03 | 云南菌视界生物科技有限公司 | Tremella aurantialba sporocarp fusion symbiotic culture method |
| CN112021070A (en) * | 2020-09-11 | 2020-12-04 | 云南菌视界生物科技有限公司 | Industrialized three-stage cultivation method of tremella aurantialba |
| CN111990164A (en) * | 2020-09-21 | 2020-11-27 | 中华全国供销合作总社昆明食用菌研究所 | Tremella aurantialba strain No. 1 in new Tremella aurantialba strains and cultivation method thereof |
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