CN108718915A - Improve the culture medium and cultural method of pleurotus edible fungus yield - Google Patents
Improve the culture medium and cultural method of pleurotus edible fungus yield Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention discloses the serial culture bases for improving pleurotus edible fungus yield, they discard branch sawdust as main component using black Lee.Wherein, culture medium A is used for elegant precious mushroom fruiting bag culture for elegant precious mushroom strain cultivation, culture medium B for elegant precious mushroom for elm mushroom and oyster mushroom strain cultivation, culture medium C, culture medium D is used for elm mushroom fruiting bag culture, and culture medium E is used for oyster mushroom fruiting bag culture.With the application of the invention, can realize Pleurotaceae edible mushroom elegant precious mushroom, elm mushroom, oyster mushroom liquid spawn directly carry out fruiting bag inoculated and cultured.Experiments have shown that culture medium and its method of the invention, which can not only reach, substitutes original culture formula as the main component with cotton seed hulls, ramulus mori, corncob, while pleurotus edible fungus yield and quality can be improved, shortens the production cycle, reduce production cost.In addition, the present invention improves the utilization rate that black Lee discards branch, realize that resource makes full use of.
Description
Technical field
The invention belongs to field of edible fungus culture, more particularly to improve culture medium and the cultivation side of pleurotus edible fungus yield
Method.
Background technology
Elegant precious mushroom (Pleurotus sajor-caju), oyster mushroom (Pleurotus ostreatus), elm mushroom
(Pleurotus citrinopileatus) belongs to Eumycota, Basidiomycetes, Agaricales, Pleurotaceae.Wherein, elegant precious mushroom with
Oyster mushroom belongs to Pleurotus, elm mushroom category gold oyster mushroom category, and three is to integrate edible, medicinal, dietotherapy Rare edible fungus
New varieties.Elegant precious mushroom shape is pleasing, fresh and tender clear and melodious, delicious flavour, rich in protein (3.65-3.88%) in fresh mushroom, ammonia
Base acid type is also more, some physiological activators which also contains, and has the synthesis for inducing interferon and improves human body and exempts from
Epidemic disease function also has the function of anti-cancer, anticancer.Elm mushroom golden yellow color is gorgeous, full of nutrition, is given birth to containing protein, a variety of dimensions
The nutritional ingredients such as element, amino acid content is particularly abundant, is rich in the necessary 8 kinds of amino acid of human body, content of glutamic acid in mushroom class with
Straw mushroom is identical.The substances such as selenium, polysaccharide body of the oyster mushroom rich in antitumor cell have very strong inhibiting effect to tumour cell, and have
There is immunological characteristic, has certain curative effect to hepatitis, chronic gastritis, gastric duodenal ulcer, osteomalacia, hypertension etc..
Three of the above pleurotus edible fungus generally uses cultivating in bag at present, is mainly cotton seed hull, corn on planting material
Core, ramulus mori etc. are usually that solid parent species → solid original seed → solid state cultivation kind → fruiting bag → goes out the production of aunt on planting technique
Flow, from parent species to the first damp mushroom, harvesting needs 120 days or so.
Black Lee (Punica granatum) is only to exist in southwest, a planting fruit-trees of South China's large-scale popularization plantation in recent years
Guilin area cultivated area has just reached 8.5 ten thousand mu.During planting black Lee, annual growth period needs to carry out with rest period
Trimming and shaping generate a large amount of discarded branches to improve fruit yield, and 100 kilograms of branches calculating are generated by trimming per acre,
Only Guilin Area generates black Lee for 1 year and gives up branch just up to 8500 tons.Current black Lee's branch waste is usually largely taken as rubbish and throws away
Fall or burn, not only result in waste of resources, also brings problem of environmental pollution.
Invention content
The technical problem to be solved in the present invention is to provide the culture mediums and cultural method that improve pleurotus edible fungus yield, should
Method can reduce the production cost of pleurotus edible fungus, shorten its production cycle, improve its yield and quality, improve simultaneously
Black Lee discards the utilization rate of branch, realizes that resource makes full use of.
In order to solve the above-mentioned technical problem, the present invention uses following technical scheme：
The culture medium for improving pleurotus edible fungus yield discards branch sawdust as main component using black Lee.
The culture medium of above-mentioned raising pleurotus edible fungus yield, containing following component potato, black Lee discard branch sawdust,
Mineral resources humic acid potassium, peptone, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose.
Culture medium A or culture medium B (being used for strain cultivation), composition are respectively：
Culture medium A often discards branch sawdust 100g, mineral resources corruption in 1000ml culture solutions containing potato 120.0g, black Lee
POTASSIUM PHYTATE 20.0g, peptone 0.5g, analysis for soybean powder 2.5g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-
900ml, is settled to 1000ml, and pH is adjusted to 6.5-7.0；
Culture medium B often discards branch sawdust 100g in 1000ml culture solutions containing potato 50.0g, black Lee, mineral resources corruption is planted
Sour potassium 10.0g, peptone 1.5g, analysis for soybean powder 2.5g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-
900ml, is settled to 1000ml, and pH is adjusted to 6.5-7.0.
The culture medium of above-mentioned raising pleurotus edible fungus yield, is prepared according to the following steps：
1. weighing potato by formula, black Lee discards branch sawdust, mineral resources humic acid potassium, peptone, analysis for soybean powder, di(2-ethylhexyl)phosphate
Hydrogen potassium, magnesium sulfate, sucrose；2. potato slice, black Lee are discarded into the particle that branch sawdust crushing is 0.1-0.2cm sizes, with
Mineral resources humic acid mixes, and adds water 700ml, boils to boiling, and boiling 10-20 minutes, filtering is maintained to remove slag, stay filtered juice；3. will weigh up
Peptone, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose be added filtrate, be mixed dissolving；4. adding water to the training of mixing
It supports in base, culture volume is finally made to reach 1000ml, pH is adjusted to 6.5-7.0.
The culture medium of above-mentioned raising pleurotus edible fungus yield discards branch sawdust, passion fruit containing black Lee of following component
Pericarp, decomposed pine soil, mineral resources humic acid potassium, land plaster, calcium superphosphate, lime.
Culture medium C, culture medium D, culture medium E (being used for fruiting bag culture), composition are respectively：
Culture medium C often discards branch sawdust 540g, paulownia waste leaf 150.0g, rice bran in 1000g culture mediums containing black Lee
100g, passion fruit pericarp 90.0g, decomposed pine soil 50g, peat soil 30g, mineral resources humic acid potassium 10.0g, land plaster 10.0g, mistake
Calcium phosphate 10.0g, lime 10.0g, water 600-700ml；
Culture medium D often discards branch sawdust 500g, paulownia waste leaf 150.0g, passion fruit in 1000g culture mediums containing black Lee
Pericarp 150.0g, cotton seed hulls 110.0g, decomposed pine soil 50g, mineral resources humic acid potassium 10g, land plaster 10.0g, calcium superphosphate
10.0g, lime 10.0g, water 600-700ml；
Culture medium E often discards branch sawdust 600.0g, passion fruit pericarp 150.0g, cotton in 1000g culture mediums containing black Lee
Sub- shell 150.0g, decomposed pine soil 60g, mineral resources humic acid potassium l0g, land plaster 10.0g, calcium superphosphate 10.0g, lime 10.0g,
Water 600-700ml.
The culture medium of above-mentioned raising pleurotus edible fungus yield, is prepared according to the following steps：
1. weighing each ingredient by formula；2. by (paulownia waste leaf, hundred perfume are mixed except other ingredients of calcium superphosphate mix
The preceding crushing of fruit pericarp)；3. calcium superphosphate dissolves in suitable water；4. will 2., 3. mix, and add water to the culture medium of mixing
In, finally make moisture content in medium up to 65% or so.
Use the cultural method of the raising pleurotus edible fungus yield of above-mentioned culture medium：
Culture medium A is used for elegant precious mushroom strain cultivation；Or culture medium B is trained for elm mushroom, oyster mushroom liquid spawn
It supports；Or culture medium C is used for elegant precious mushroom fruiting bag culture；Or culture medium D is used for elm mushroom fruiting bag culture；Or by culture medium
E is used for oyster mushroom fruiting bag culture.
The cultural method of above-mentioned raising pleurotus edible fungus yield：
(1) culture medium A, B are sub-packed in the vial of 1000ml, per bottled culture solution 500ml, 120 DEG C sterilize 40 minutes；
After sterilizing after culture solution is cooled to room temperature, the solid parent species of access about 1.0cm × 1.0cm × 1.0cm sizes, 25-28 DEG C
Shaking table culture at room temperature, shaking table shake fast 115-120 revs/min, and incubation time 7-8 days obtains level liquid strain；
(2) culture medium A, B are sub-packed in the fermentation tank of 300L, per canned culture solution 200L, sterilized 60 minutes at 120 DEG C；
After sterilizing after culture solution is cooled to room temperature, about 300ml liquid parent species are accessed per tank, 25-28 DEG C is cultivated at room temperature；Culture period
Between maintain tank that 0.03-0.05mPa, gas is pressed to build pressure gauge 0.15mPa or so, incubation time 5-6 days obtains secondary liquid strain；
(3) culture medium C, D, E are sub-packed in 19cm × 40cm polypropylene plastics pockets, per packed siccative about 400g, use nest
After mouth machine nest mouth, sealed with the tampon that chemical fibre cotton is done, sterilization 240 minutes, then take out at 120 DEG C, and bacterium bag is cooled to
Room temperature is for use；The bacterium bag of the postcooling that sterilizes to room temperature moves into transfer room inoculation, and 30ml secondary liquid strains are accessed per culture bag, are connect
22-26 DEG C of culture 15-20 days or so at room temperature in bacterium bag, obtains the fruiting bag for covering with mycelia after kind.
Fruiting period management is carried out by following operation：
Elegant precious mushroom fruiting period management ---
After the long purseful of oyster mushroom pieces, continues to place 5-7 days in culturing room, so that mycelia is reached physiological maturity, then transport to
Mushroom house fruiting；Bacterium bag is piled up, 5 layers totally into behind room using single wall mode, after bacterium bag code is good, is opened sack, is beaten with cold is beaten
Cold 2 days, 10 DEG C of day and night temperature (12 DEG C of evening temperature, 22 DEG C of day temperature) was kept therebetween；Beat it is cold after, mushroom house temperature protect
It holds at 16-25 DEG C, relative air humidity keeps 85-90%, and ventilation 2 times, 30 minutes every time, are given once daily certain diffusion daily
Light；It beats on cold rear charge level visible after 3-5 days and forms mushroom former base, continue the air humidity for keeping 85-90%, ventilation increase daily is
3 times, every time 30 minutes；When the bacteria cover diameter of elegant precious mushroom reaches 3 centimetres or more, harvesting；
First tide removes fructification residue after adopting, and cuts off the water bacteria after 5 days or so, then sprays time heavy water, and cold urge is beaten after 2-3 days
Flower bud then proceedes to that mushroom house temperature is made to be maintained at 16-25 DEG C, air humidity 85%-95%, daily ventilation 2-3 times, every time 30 points
Clock；It can adopt 3 times；
Elm mushroom management of producing mushroom ---
After the long purseful of mycelia, continue to place 5-7 days in culturing room, moves into mushroom room and carry out management of producing mushroom；Bacterium bag is using single
Row's wall mode is piled up, and totally 5 layers, sack is opened after bacterium bag code is good；Bacterium bag puts mushroom house about after a week, and button will largely occur；
Mushroom house temperature is maintained at 18-26 DEG C, air humidity 85%-95% during fruiting, daily ventilation 2-3 times, 30 minutes every time, and elm is yellow
Mushroom needs 8~10 days from buddingging to harvesting；First tide removes fructification residue after adopting, and cuts off the water bacteria after 5 days or so, then sprays time weight
Water then proceedes to that mushroom house temperature is made to be maintained at 18-26 DEG C, air humidity 85%-95%, daily ventilation 2-3 times, every time 30 points
Clock；It can adopt 3 times；
Oyster mushroom fruiting period management ---
After the long purseful of hypha of Pleurotus ostreatus, continue to place 5-7 days in culturing room, moves into mushroom room and carry out management of producing mushroom；Bacterium bag is adopted
It is piled up with single wall mode, totally 6 layers, sack is opened after bacterium bag code is good；Bacterium bag puts mushroom house about after a week, and button will measure greatly
It is existing；Mushroom house temperature is maintained at 18-26 DEG C, air humidity 85%-95% during fruiting, daily ventilation 2-3 times, 30 minutes every time,
Oyster mushroom needs 8~10 days from buddingging to harvesting；First tide removes fructification residue after adopting, and cuts off the water bacteria after 5 days or so, then sprays secondary
Heavy water then proceedes to that mushroom house temperature is made to be maintained at 18-26 DEG C, air humidity 85%-95%, daily ventilation 2-3 times, every time 30 points
Clock；It can adopt 3 times.
The problems such as long, of high cost there are the period for current pleurotus edible fungus solid state cultivation, inventor is useless using black Lee
It is wide and the characteristics of containing a large amount of lignin, cellulose and mineral element to abandon branch source, as Edible Fungi raw material,
The serial culture base for improving pleurotus edible fungus yield is had developed by design screening, they are with the discarded branch sawdust of black Lee
Main component.Wherein, culture medium A is used for elm mushroom and oyster mushroom for elegant precious mushroom for elegant precious mushroom strain cultivation, culture medium B
Strain cultivation, culture medium C are used for elegant precious mushroom fruiting bag culture, and culture medium D is used for elm mushroom fruiting bag culture, culture medium E
For oyster mushroom fruiting bag culture.With the application of the invention, the liquid spawn of Pleurotaceae edible mushroom elegant precious mushroom, elm mushroom, oyster mushroom can be realized
Directly carry out fruiting bag inoculated and cultured.Accordingly, inventor has also set up corresponding cultural method, and level liquid bacteria culture fluid is matched
System is inoculated with and is prepared with cultural hypha → secondary liquid bacteria culture fluid, is inoculated with and prepares, connect with cultural hypha → fruiting bag culture medium
Kind and fruiting bag mycelia afterripening → mushroom flower bud formation → fruiting phase temperature, the moisture management → harvesting etc. for cultivating → covering with mycelia
Step.Experiments have shown that culture medium and its method of the invention can not only reach substitute it is original with cotton seed hulls, ramulus mori, corn
Core culture formula as the main component, while pleurotus edible fungus yield and quality can be improved, shorten the production cycle, reduce life
Produce cost.In addition, the present invention improves the utilization rate that black Lee discards branch, realize that resource makes full use of.
Compared with prior art, outstanding advantage of the invention is：
(1) culture medium prescription using the present invention, biological transformation ratio is above control, while mycelial growth rate has centainly
Quickening.
In the case of conventional formulation：The biological conversion rate that elegant precious mushroom one recommends mushroom is up to 96.8%, compares control formula
The one average organism conversion ratio for recommending mushroom is higher by 14.4% or so；The biological conversion rate that elm mushroom one recommends mushroom is up to 127.7%, than
The average organism conversion ratio that control formula one recommends mushroom is higher by 25.9% or so；The biological conversion rate that oyster mushroom one recommends mushroom is up to
135.2%, the average organism conversion ratio that mushroom is recommended than control formula one is higher by 25.9% or so.
In addition, black Lee discards branch sawdust with passion fruit pericarp as the produced elegant precious mushroom of compost, elm mushroom, oyster mushroom product
Matter is preferable, and content of soluble protein reaches 8.3mg/g, 7.4mg/g, 6.2mg/g, soluble sugar content point respectively in fructification
Do not reach 7.5mg/g, 5.2mg/g, 4.5mg/g, is above control.
(2) fluid present invention strain formula relatively control mycelial yield is high, and sowing quantity is less than control, opposite to have saved cost,
Growth cycle shortens simultaneously.
(3) production cost of the present invention declines, and production efficiency improves.When elegant precious mushroom, elm mushroom, oyster mushroom use solid spawn,
Inoculation efficiency is 490 bags/6 people/hour, bacterium bag pollution rate 4.2%；After liquid spawn using the present invention, inoculation efficiency is
4000 bags/6 people/hour, bacterium bag pollution rate 1.5%, inoculation efficiency improves 32.7% than control.
(4) black Lee of culture medium discards branch sawdust content up to 50% or more in the present invention, and cost of material is made to be declined,
The added value of relevant industries is improved simultaneously, and has facilitation to circular economy and environmental protection.
Specific implementation mode
1 elegant precious mushroom high-yield culturing of embodiment
(1) culture medium is prepared
Culture medium A often discards branch sawdust 100g, mineral resources corruption in 1000ml culture solutions containing potato 120.0g, black Lee
POTASSIUM PHYTATE 20.0g, peptone 0.5g, analysis for soybean powder 2.5g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-
900ml, is settled to 1000ml, and pH is adjusted to 6.5-7.0.
Culture medium A is prepared according to the following steps：1. weighing potato by quality, black Lee discards branch sawdust, mineral resources humic acid
Potassium, peptone, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose；2. discarding branch sawdust and (crushing potato slice, black Lee
For the particle of 0.1-0.2cm sizes) it is mixed with mineral resources humic acid potassium person, add water 700ml, boils to boiling, maintain boiling 20 minutes,
Filtering, removes slag, stays filtered juice；3. filtrate, mixing is added in the peptone weighed up, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose
Stirring and dissolving；4. adding water in the culture medium of mixing, culture volume is finally made to reach 1000ml, pH is adjusted to 6.5-7.0.
Culture medium C is used for elegant precious mushroom fruiting bag cultural hypha, contains the discarded branch sawdust of black Lee in every 1000g culture mediums
540g, paulownia waste leaf 150.0g, rice bran 100g, passion fruit pericarp 90.0g, decomposed pine soil 50g, peat soil 30g, mineral resources corruption are planted
Sour potassium 10.0g, land plaster 10.0g, calcium superphosphate 10.0g, lime 10.0g, water 600-700ml.
Culture medium C is prepared according to the following steps：1. weighing black Lee by quality discards branch sawdust, paulownia waste leaf, rice bran, hundred perfume
Fruit pericarp, decomposed pine soil, peat soil, mineral resources humic acid potassium, land plaster, calcium superphosphate, lime；2. black Lee is discarded branch wood
Bits, this 9 kinds of paulownia waste leaf, passion fruit pericarp, decomposed pine soil, rice bran, peat soil, mineral resources humic acid potassium, land plaster, lime at
Divide to mix and mix；3. calcium superphosphate dissolves in suitable water；4. will 2., 3. mix, and add water to the culture medium of mixing
In, finally make moisture content in medium up to 65% or so.
(2) packing of culture medium A, sterilization, inoculation
Culture medium A is sub-packed in the vial of 1000ml, per bottled culture solution 500ml, 120 DEG C sterilize 40 minutes, sterilizing
Wild Oryza species are cooled to room temperature, and access the solid parent species of about 1.0cm × 1.0cm × 1.0cm sizes, 25-28 DEG C is shaken at room temperature
Bed culture, shaking table shake 120 revs/min of speed, and incubation time 7-8 days obtains the level liquid that mycelial density accounts for culture solution 75% or so
Strain.
(3) packing of culture medium A, sterilization, inoculation
Culture solution A is loaded on to the fermentation tank of 300L, per canned culture solution 200L, 60 minutes at 120 DEG C, culture solution after sterilizing
It is cooled to room temperature, the level liquid strain of about 300ml is accessed per tank, 25-28 DEG C is cultivated at room temperature；Tank pressure is maintained during culture
0.03-0.05mPa.Incubation time 5-6 days obtains the secondary liquid strain that mycelium pellet density accounts for 75% or so；
(4) packing of culture medium C, sterilization, inoculation
Culture medium C is loaded in 19cm × 40cm polypropylene plastics pockets, per packed siccative about 400g, with socket machine nest mouth
Afterwards, it is sealed with chemical fibre cotton, sterilization 240 minutes, then take out at 120 DEG C, are cooled to room temperature.Transfer room is moved into later to connect
Kind.Every bag of access 30ml liquid spawn.
(5) fruiting bag culture
Fruiting bag equipped with culture medium C is cultivated at 22-26 DEG C, and incubation time 15-20 days obtains the fruiting for covering with mycelia
Bag.
(6) management of producing mushroom
After the long purseful of oyster mushroom pieces, continues to place 5-7 days in culturing room, so that mycelia is reached physiological maturity, then transport to
Mushroom house fruiting.Bacterium bag is piled up, 5 layers totally into after mushroom room using single wall mode, after bacterium bag code is good, opens sack, cold with beating
Machine is beaten cold 2 days, keeps 10 DEG C of day and night temperature (12 DEG C of evening temperature, 22 DEG C of day temperature) therebetween.Beat it is cold after, mushroom house temperature
Degree is maintained at 16-25 DEG C, and relative air humidity keeps 85-90%, and ventilation 2 times, 30 minutes every time, are given once daily certain daily
Diffused light.It beats cold rear sack surface visible after 3-5 days and forms mushroom former base, continue the air humidity for keeping 85-90%, divulge information daily
Increase is 3 times, every time 30 minutes.When the bacteria cover diameter of elegant precious mushroom reaches 3 centimetres or more, harvesting.
First damp mushroom removes fructification residue after adopting, and cuts off the water bacteria after 5 days or so, then sprays time heavy water, is beaten after 2-3 days cold
Flower bud, then by continuing that mushroom house temperature is made to be maintained at 16-25 DEG C, air humidity 85%-95%, ventilation 2-3 times daily, every time 30
Minute.2 tides are adopted altogether.
As a result：First damp mushroom biological transformation ratio 83.4%, the second damp mushroom biological transformation ratio 38.0% harvest two tides, always altogether
Biological transformation ratio 121.4%.
Embodiment 2 is using in the case of liquid spawn, and elegant precious mushroom traditional cultivation culture medium is with culture medium of the present invention to fruiting
Bag mycelial growth rate, biological transformation ratio, Fruitbody comparative test
(1) mushroom producing culture base C of the present invention discards the culture medium of branch sawdust in mycelia growth, life with black Lee is individually added
Comparison on object conversion ratio
Culture medium prescription 1 (is denoted as training 1)：Black Lee discard branch sawdust 97%, land plaster 1.0%, calcium superphosphate 1.0%,
Lime 1.0%；
Culture medium prescription 2 (is denoted as training 2)：Black Lee discards branch sawdust 87%, rice bran 10%, land plaster 1.0%, peroxophosphoric acid
Calcium 1.0%, lime 1.0%；
Culture medium prescription C (being denoted as this) of the present invention：Black Lee discards branch sawdust 54.0%, paulownia waste leaf 15.0%, rice bran
10.0%, passion fruit pericarp 9.0%, decomposed pine soil 5.0%, peat soil 3.0%, mineral resources humic acid potassium 1.0%, land plaster
1.0%, calcium superphosphate 1.0%, lime 1.0%.
Fruiting bag culture medium is carried out with reference to embodiment 1, and is packed (in 19cm × 40cm polypropylene plastics pockets, per packed dry
Expect about 400g), sterilizing, then liquid spawn is taken to be inoculated with, culture bag is cultivated 15-20 days or so in 22-26 DEG C of culturing room.
1 black Lee of table discards branch sawdust different ratio and compares the growth of fruiting bag mycelia, the influence of biological transformation ratio
* 1 often handles 150 bacterium bags, 3 repetitions；Table 2,5,6,8 is same.
As can be seen from Table 1, in the case where individually adding black Lee and discarding the condition of culture of branch sawdust, mycelial growth rate is slower,
The growing way of mycelia is also weaker；After black Lee discards branch sawdust 20% rice bran of addition, mycelial growth rate is accelerated, the length of mycelia
Gesture enhances, and biological transformation ratio is also improved；Best culture medium prescription is that black Lee discards based on branch sawdust, and addition paulownia are useless
The culture medium C of the present invention as main component such as leaf, rice bran, passion fruit pericarp, decomposed pine soil, the formula grow speed in mycelia
Degree, mycelium growth vigor are better than other 2 processing on biological transformation ratio.
(2) traditional fruiting bag culture medium and mushroom producing culture base C mycelia growth of the present invention, biological transformation ratio, Fruitbody
Compare
Traditional fruiting bag culture medium prescription 1 (being denoted as biography 1)：Eucalyptus bits 50%, cotton seed hull 35%, rice bran 24%, land plaster
1.0%, calcium superphosphate 1.0%；
Traditional fruiting bag culture medium prescription 2 (being denoted as biography 2)：Corncob 50%, ramulus mori 35%, rice bran 24%, land plaster
1.0%, calcium superphosphate 1.0%；
Culture medium prescription C (being denoted as this) of the present invention：Black Lee discards branch sawdust 54.0%, paulownia waste leaf 15.0%, rice bran
10.0%, passion fruit pericarp 9.0%, decomposed pine soil 5.0%, peat soil 3.0%, mineral resources humic acid potassium 1.0%, land plaster
1.0%, calcium superphosphate 1.0%, lime 1.0%.
Fruiting bag culture medium is carried out with reference to embodiment 1, and is packed (in 19cm × 40cm polypropylene plastics pockets, per packed dry
Expect about 400g), sterilizing, then liquid spawn is taken to be inoculated with, culture bag is cultivated 15-20 days in 22-26 DEG C of culturing room.
2 different disposal culture medium fruiting bag mycelia growth of table, the influence of biological transformation ratio, Fruitbody are compared
* 1 fructification content of soluble protein measures uses Coomassie brilliant blue colorimetric method；Fructification soluble reducing sugars contain
It measures and uses Sulphuric acid-anthrone colorimetry surely.Table 6,9 is same.
As can be seen from Table 2, the time used in the long purseful of mycelia of fruiting bag culture medium C of the present invention cultivates than tradition
Base is obviously shortened；Total biological transformation ratio of fruiting bag culture medium C of the present invention is apparently higher than conventional method, fructification
Quality also increases.
3 traditional liquid strain mycelium morphology factor of embodiment is compared with culture medium A mycelium morphology factor of the present invention
Traditional liquid Spawn incubation based formulas 1 (being denoted as biography 1)：Potato 20.0%, potassium dihydrogen phosphate 0.3%g, magnesium sulfate
0.15%g, sucrose 2.5%；
Traditional liquid Spawn incubation based formulas 2 (being denoted as biography 2)：Potato 20.0%, corn flour 8.0%, potassium dihydrogen phosphate
0.3%g, magnesium sulfate 0.15%g, 2.5% percarbonic acid calcium 1% of sucrose, land plaster 1.0%；
Fluid present invention Spawn incubation based formulas A (is denoted as this)：Potato 12.0%, black Lee discard branch sawdust
10.0%, mineral resources humic acid potassium 2.0%, peptone 0.05%, analysis for soybean powder 0.25%, potassium dihydrogen phosphate 0.3%, magnesium sulfate
0.15%, sucrose 2.5.
Liquid spawn culture medium is carried out with reference to embodiment 1, and bottle (the triangular glass bottle of 1000ml, every bottle of culture solution
500ml), it sterilizes, then parent species is taken to be inoculated with, culture bottle is cultivated 8 days in 25-28 DEG C of culturing room.Culture existed bacterium solution after 8 days
4000rpm is centrifuged, and removes supernatant, claims mycelia weight of precipitate.
The growth of 3 different components fluid nutrient medium mycelia of table is compared
* 10 bottles are often handled, 3 repetitions.Table 7,10 is same.
As can be seen from Table 3, the mycelium morphology factor of liquid spawn culture medium A of the present invention has bright than traditional culture solution
Aobvious to improve, mycelium pellet forms the time also earlier than conventional formulation.
4 fluid present invention strain of embodiment is inoculated with fruiting bag and is given birth to fruiting bag with conventional solid inoculation fruiting bag inoculation speed
Compare for a long time
Fruiting bag culture medium C is carried out with reference to embodiment 1, and is packed (in 19cm × 40cm polypropylene plastics pockets, per packed dry
Expect about 400g), sterilizing, be then inoculated with solid spawn and the liquid spawn inoculation of the present invention respectively, culture bag cultivates at 22-26 DEG C
It cultivates room.
Influence of the 4 different type strain of table to elegant precious mushroom production efficiency
As seen from Table 4, using liquid spawn inoculation efficiency be far above solid spawn, while mycelia cover with bacterium bag time it is big
For shorten, but under same culture medium two class strains biological transformation ratio indifference.
5 elm mushroom high-yield culturing of embodiment
(1) culture medium is prepared
Culture medium B often contains in 1000ml culture solutions and discards branch sawdust 100g, mineral resources containing potato 50.0g, black Lee
Humic acid potassium 10g, peptone 1.5g, analysis for soybean powder 2.5g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-
900ml is settled to 1000ml, pH6.5-7.0.
Culture medium B is prepared according to the following steps：1. weighing potato by quality, black Lee discards branch sawdust, mineral resources humic acid
Potassium, peptone, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose；2. potato slice, black Lee are discarded branch sawdust and mineral resources
Humic acid potassium mixes, and adds water 700ml, boils to boiling, and boiling 10 minutes, filtering is maintained to remove slag, stay filtered juice；3. the egg that will be weighed up
Filtrate is added in white peptone, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose, and dissolving is mixed；4. adding water to the culture medium of mixing
In, so that culture volume is reached 1000ml, pH is adjusted to 6.5-7.0.
Culture medium D is used for fruiting bag culture, gives up containing the discarded branch sawdust 500g of black Lee, paulownia in every 1000g culture mediums
Leaf 150.0g, passion fruit pericarp 150.0g, cotton seed hulls 110.0g, decomposed pine soil 50g, mineral resources humic acid potassium 10g, land plaster
10.0g, calcium superphosphate 10.0g, lime 10.0g, water 600-700ml.
Culture medium D is prepared according to the following steps：1. weighing black Lee by quality discards branch sawdust, paulownia waste leaf, passion fruit fruit
Skin, cotton seed hulls, decomposed pine soil, mineral resources humic acid potassium, land plaster, calcium superphosphate, lime；2. by black Lee discard branch sawdust,
This 8 kinds of ingredients of paulownia waste leaf, passion fruit pericarp, cotton seed hulls, decomposed pine soil, mineral resources humic acid potassium, land plaster, lime are mixed in one
It rises and mixes；3. calcium superphosphate dissolves in suitable water；4. will 2., 3. mix, and add water in the culture medium of mixing, finally makes
Moisture content in medium is up to 65% or so.
(2) packing of culture medium B, sterilization, inoculation
Culture medium B is sub-packed in the vial of 1000ml, every bottle of culture solution 500ml, 120 DEG C sterilize 40 minutes, after sterilizing
Culture medium is cooled to room temperature, and accesses the solid parent species of about 1.0cm × 1.0cm × 1.0cm sizes, 25-28 DEG C of shaking table at room temperature
Culture, shaking table shake 120 revs/min of speed, and incubation time 7-8 days obtains level liquid strain；
(3) packing of culture medium B, sterilization, inoculation
Culture solution B is sub-packed in the fermentation tank of 300L, per canned culture solution 200L, 60 minutes at 120 DEG C, is cultivated after sterilizing
Liquid is cooled to room temperature, and about 300ml level liquid strains is accessed per tank, 25-28 DEG C is cultivated at room temperature.Tank is maintained to press 0.03-
0.05mPa, gas build pressure gauge 0.15mPa or so.Incubation time 5-6 days obtains secondary liquid strain；
(4) packing of culture medium D, sterilization, inoculation
The culture medium D prepared is sub-packed in 19cm × 40cm polypropylene plastics pockets, per packed siccative about 400g, with nest mouth
It after machine nest mouth, is sealed with sponge, sterilization 240 minutes, then take out at 120 DEG C, are cooled to room temperature for use.It is cold after sterilizing
But to room temperature, transfer room inoculation is moved into.Every bag of access 30ml liquid spawn.22-26 DEG C is cultivated at room temperature, incubation time 15-20
It or so obtains the fruiting bag for covering with mycelia；
(5) management of producing mushroom
After the long purseful of mycelia, continue to place 5-7 days in culturing room, moves into mushroom room and carry out management of producing mushroom.Bacterium bag is using single
Row's wall mode is piled up, and totally 5 layers, sack is opened after bacterium bag code is good.Bacterium bag puts mushroom house about after a week, and button will largely occur.
Mushroom house temperature is maintained at 18-26 DEG C, air humidity 85%-95% during fruiting, daily ventilation 2-3 times, 30 minutes every time, and elm is yellow
Mushroom needs 8-10 days from buddingging harvesting.First tide removes fructification residue after adopting, and cuts off the water bacteria after 5 days or so, then sprays time weight
Water then proceedes to that mushroom house temperature is made to be maintained at 18-26 DEG C, air humidity 85%-95%, daily ventilation 2-3 times, every time 30 points
Clock.It adopts altogether 2 times.
As a result：First damp mushroom biological transformation ratio 94.6%, the second damp mushroom biological transformation ratio 32.8% harvest two tides, always altogether
Biological transformation ratio 127.4%.
6 elm mushroom traditional cultivation culture medium of embodiment turns fruiting bag mycelial growth rate and biology with culture medium of the present invention
Rate comparative test
(1) mushroom producing culture base D of the present invention discards the culture medium of branch sawdust in mycelia growth, life with black Lee is individually added
Comparison on object conversion ratio
Culture medium prescription 1 (is denoted as training 1)：Black Lee discard branch sawdust 97%, land plaster 1.0%, calcium superphosphate 1.0%,
Lime 1.0%；
Culture medium prescription 2 (is denoted as training 2)：Black Lee discards branch sawdust 87%, rice bran 10%, land plaster 1.0%, peroxophosphoric acid
Calcium 1.0%, lime 1.0%；
Culture medium prescription D (being denoted as this) of the present invention：It is fragrant that black Lee discards branch sawdust 50.0%, paulownia waste leaf 15.0%, hundred
Fruit pericarp 15.0%, decomposed pine soil 5.0%, mineral resources humic acid potassium 1.0%, land plaster 1.0%, crosses phosphorus at cotton seed hulls 11.0%
Sour calcium 1.0%, lime 1.0%.
Fruiting bag culture medium is carried out with reference to embodiment 5, and is packed (in 19cm × 40cm polypropylene plastics pockets, per packed dry
Expect about 400g), sterilizing, then liquid spawn is taken to be inoculated with, culture bag is cultivated 15-20 days or so in 22-26 DEG C of culturing room.Table 5 is black
Lee discards branch sawdust compared with passion fruit skin different ratio is to the growth of fruiting bag mycelia, the influence of biological transformation ratio
As can be seen from Table 5, in the case where individually adding black Lee and discarding the condition of culture of branch sawdust, mycelial growth rate is slower,
The growing way of mycelia is also weaker；After black Lee discards branch sawdust 20% rice bran of addition, mycelial growth rate is accelerated, the length of mycelia
Gesture enhances, and biological transformation ratio is also improved；Best culture medium prescription is that black Lee discards based on branch sawdust, and addition paulownia are useless
The culture medium D of the present invention of leaf, passion fruit pericarp, cotton seed hulls, decomposed pine soil, mineral resources humic acid potassium, the formula are grown in mycelia
Speed, mycelium growth vigor are better than other 2 processing on biological transformation ratio.
(2) the traditional fruiting bag culture medium prescription of liquid spawn progress using the present invention is matched with mushroom producing culture base D of the present invention
The growth of Fang Jinhang fruiting bag mycelia, biological transformation ratio compare
Traditional fruiting bag culture medium prescription 1 (being denoted as biography 1)：Cotton seed hull 85%, land plaster 1.0%, crosses phosphorus at rice bran 17.0%
Sour calcium 1.0%, lime 1%；
Traditional fruiting bag culture medium prescription 2 (being denoted as biography 2)：Weed tree sawdust 55%, corncob 22%, rice bran 20%, land plaster
1.0%, calcium superphosphate 1.0%, lime 1%；
Fruiting bag culture medium prescription D (being denoted as this) of the present invention：Black Lee discards branch sawdust 50.0%, paulownia waste leaf
15.0%, passion fruit pericarp 15.0%, cotton seed hulls 11.0%, decomposed pine soil 5.0%, mineral resources humic acid potassium 1.0%, land plaster
1.0%, calcium superphosphate 1.0%, lime 1.0%.
Fruiting bag culture medium is carried out with reference to embodiment 5, and is packed (in 19cm × 40cm polypropylene plastics pockets, per packed dry
Expect about 400g), sterilizing, then liquid spawn is taken to be inoculated with, culture bag is cultivated 15-20 days or so in 22-26 DEG C of culturing room.
6 different disposal culture medium fruiting bag mycelia growth of table, the influence of biological transformation ratio, Fruitbody are compared
As can be seen from Table 6, the time used in the long purseful of mycelia of the fruiting bag of culture medium D of the present invention trains than tradition
It supports and is obviously shortened.Total biological transformation ratio of the fruiting bag of culture medium D of the present invention is apparently higher than conventional method.
The traditional elm mushroom liquid spawn mycelium morphology factor of embodiment 7 is compared with culture medium B mycelium morphology factors of the present invention
Traditional liquid Spawn incubation based formulas 1 (being denoted as biography 1)：Potato 20.0%, potassium dihydrogen phosphate 0.3%g, magnesium sulfate
0.15%g, sucrose 2.5%；
Traditional liquid Spawn incubation based formulas 2 (being denoted as biography 2)：Potato 20.0%, corn flour 8.0%, potassium dihydrogen phosphate
0.3%g, magnesium sulfate 0.15%g, 2.5% percarbonic acid calcium 1% of sucrose, land plaster 1.0%；
Fluid present invention Spawn incubation based formulas B (is denoted as this)：Potato 5.0% is weighed by quality, black Lee discards branch
Sawdust 10.0%, mineral resources humic acid potassium 1.0%, peptone 0.15%, analysis for soybean powder 0.25%, potassium dihydrogen phosphate 0.3%, magnesium sulfate
0.15%, sucrose 2.5%.
Liquid spawn culture medium is carried out with reference to embodiment 5, and bottle (the triangular glass bottle of 1000ml, every bottle of culture solution
500ml), it sterilizes, solid parent species is then taken to be inoculated with, culture bottle is cultivated 7 days in 25-28 DEG C of culturing room
The growth of 7 different components fluid nutrient medium mycelia of table is compared
As can be seen from Table 7, the mycelium morphology factor of fluid nutrient medium B of the present invention has bright than tradition culture formula of liquid
Aobvious to improve, mycelium pellet forms the time also earlier than conventional formulation.
8 High Yielding Cultivation of Pleurotus ostreatus of embodiment
(1) culture medium is prepared
Culture medium B often contains in 1000ml culture solutions and discards branch sawdust 100g, mineral resources containing potato 50.0g, black Lee
Humic acid potassium 10g, peptone 1.5g, analysis for soybean powder 2.5g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-
900ml is settled to 1000ml, pH6.5-7.0.
Culture medium B is prepared according to the following steps：1. weighing potato by quality, black Lee discards branch sawdust, mineral resources humic acid
Potassium, peptone, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose；2. potato slice, black Lee are discarded branch sawdust and mineral resources
Humic acid potassium mixes, and adds water 700ml, boils to boiling, and boiling 10 minutes, filtering is maintained to remove slag, stay filtered juice；3. the egg that will be weighed up
Filtrate is added in white peptone, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose, and dissolving is mixed；4. adding water to the culture medium of mixing
In, so that culture volume is reached 1000ml, pH is adjusted to 6.5-7.0.
Culture medium E is used for oyster mushroom fruiting bag cultural hypha, contains the discarded branch sawdust of black Lee in every 1000g culture mediums
600.0g, passion fruit pericarp 150.0g, cotton seed hull 150.0g, decomposed pine soil 60g, mineral resources humic acid potassium 10g, land plaster
10.0g, calcium superphosphate 10.0g, lime 10.0g, water 600-700ml.
Culture medium E is prepared according to the following steps：1. by quality weigh black Lee discard branch sawdust, passion fruit pericarp, cotton seed hull,
Decomposed pine soil, mineral resources humic acid potassium, land plaster, calcium superphosphate, lime；2. by black Lee discard branch sawdust, passion fruit pericarp,
This 8 kinds of ingredients of cotton seed hull, decomposed pine soil, mineral resources humic acid potassium, land plaster, lime, which mix, to be mixed；3. calcium superphosphate exists
It is dissolved in suitable water；4. will 2., 3. mix, and add water in the culture medium of mixing, finally makes moisture content in medium up to 65%
Left and right.
(2) packing of culture medium B, sterilization, inoculation
Culture medium B is sub-packed in the vial of 1000ml, every bottle of culture solution 500ml, 120 DEG C sterilize 40 minutes, after sterilizing
Culture medium is cooled to room temperature, and accesses the solid parent species of about 1.0cm × 1.0cm × 1.0cm sizes, 25-28 DEG C of shaking table at room temperature
Culture, shaking table shake 120 revs/min of speed, and incubation time 7-8 days obtains the level liquid bacterium that mycelial density accounts for culture solution 75% or so
Kind.
(3) packing of culture medium B, sterilization, inoculation
Culture solution B is sub-packed in the fermentation tank of 300L, per canned culture solution 200L, 60 minutes at 120 DEG C, is cultivated after sterilizing
Liquid is cooled to room temperature, and about 300ml liquid parent species is accessed per tank, 25-28 DEG C is cultivated at room temperature.Tank is maintained to press 0.03-
0.05mPa, gas build pressure gauge 0.15mPa or so.Incubation time 5-6 days obtains the two level liquid that mycelium pellet density accounts for 75% or so
Body strain；
(4) packing of culture medium E, sterilization, inoculation
By the culture medium E prepared loaded in 19cm × 40cm polypropylene plastics pockets, per packed siccative about 400g, socket machine is used
It after nest mouth, is sealed with sponge, sterilization 240 minutes, then take out at 120 DEG C, are cooled to room temperature for use.Sterilize postcooling
To room temperature, transfer room inoculation is moved into.Every bag of access 30ml liquid spawn.
Fruiting bag culture
It is cultivated at 22-26 DEG C, incubation time 15-20 days or so obtains the fruiting bag for covering with mycelia
Management of producing mushroom
After the long purseful of mycelia, continue to place 5-7 days in culturing room, moves into mushroom room and carry out management of producing mushroom.Bacterium bag is using single
Row's wall mode is piled up, and totally 6 layers, sack is opened after bacterium bag code is good.Bacterium bag puts mushroom house about after a week, and button will largely occur.
Mushroom house temperature is maintained at 18-26 DEG C, air humidity 85%-95% during fruiting, daily ventilation 2-3 times, 30 minutes every time, oyster mushroom
It is needed 8~10 days from buddingging to harvesting.First tide removes fructification residue after adopting, and cuts off the water bacteria after 5 days or so, then sprays time weight
Water then proceedes to that mushroom house temperature is made to be maintained at 18-26 DEG C, air humidity 85%-95%, daily ventilation 2-3 times, every time 30 points
Clock.It adopts altogether 2 times.
As a result：First damp mushroom biological transformation ratio 98.5%, the second damp mushroom biological transformation ratio 35.6% harvest two tides, always altogether
Biological transformation ratio 134.1%.
9 oyster mushroom traditional cultivation culture medium of embodiment is with culture medium of the present invention to fruiting bag mycelial growth rate, bioconversion
Rate, Fruitbody comparative test
(1) mushroom producing culture base E of the present invention discards branch sawdust medium in mycelia growth, biology with black Lee is individually added
Comparison on conversion ratio
Culture medium prescription 1 (is denoted as training 1)：Black Lee discard branch sawdust 97%, land plaster 1.0%, calcium superphosphate 1.0%,
Lime 1.0%；
Culture medium prescription 2 (is denoted as training 2)：Black Lee discards branch sawdust 87%, rice bran 10%, land plaster 1.0%, peroxophosphoric acid
Calcium 1.0%, lime 1.0%；
Culture medium prescription E (being denoted as this) of the present invention：Black Lee discards branch sawdust 60.0%, passion fruit pericarp 15.0%g, cotton
Sub- shell 15.0%, decomposed pine soil 6.0%, mineral resources humic acid potassium 1.0%, land plaster 1.0%, calcium superphosphate 1.0%, lime
1.0%.
Fruiting bag culture medium is carried out with reference to embodiment 8, and is packed (in 19cm × 40cm polypropylene plastics pockets, per packed dry
Expect then about 400g, sterilizing take liquid spawn to be inoculated with, culture bag is cultivated 15-20 days or so in 22-26 DEG C of culturing room.
8 black Lee of table discards shadow of the branch sawdust with passion fruit skin different ratio to the growth of fruiting bag mycelia, biological transformation ratio
It rings and compares
As can be seen from Table 8, in the case where individually adding black Lee and discarding the condition of culture of branch sawdust, mycelial growth rate is slower,
The growing way of mycelia is also weaker；After black Lee discards branch sawdust 20% rice bran of addition, mycelial growth rate is accelerated, the length of mycelia
Gesture enhances, and biological transformation ratio is also improved；Best culture medium prescription is that black Lee discards branch sawdust, passion fruit pericarp, cotton seed
Shell culture medium E of the present invention as main component, the formula are better than on mycelial growth rate, mycelium growth vigor, biological transformation ratio
Other 2 processing.
(2) traditional fruiting bag culture medium is grown with fruiting bag culture medium E mycelia of the present invention, compared with biological transformation ratio
Traditional fruiting bag culture medium prescription 1 (being denoted as biography 1)：Cotton seed hull 75.0%, rice bran 22.0%, land plaster 1.0%, mistake
Calcium phosphate 1.0%；
Traditional fruiting bag culture medium prescription 2 (being denoted as biography 2)：Cotton seed hull 55.0%, corncob 35.0%, rice bran 8.0%, stone
Cream powder 1.0%, calcium superphosphate 1.0%；
Fruiting bag culture medium prescription E (being denoted as this) of the present invention：Black Lee discards branch sawdust 60.0%, passion fruit pericarp
15.0%g, cotton seed hull 15.0%, decomposed pine soil 6.0%, mineral resources humic acid potassium 1.0%, land plaster 1.0%, calcium superphosphate
1.0%, lime 1.0%.
Fruiting bag culture medium is carried out with reference to embodiment 8, and packs (19cm × 40cm polypropylene plastics pockets, per packed siccative
About 400g, sterilizing, then takes liquid spawn to be inoculated with, and culture bag is cultivated 15-20 days in 22-26 DEG C of culturing room
9 different disposal culture medium fruiting bag mycelia growth of table, the influence of biological transformation ratio, Fruitbody are compared
As can be seen from Table 9, the time used in the long purseful of mycelia of fruiting bag culture medium E of the present invention cultivates than tradition
It is obviously shortened.Total biological transformation ratio of fruiting bag culture medium E of the present invention is apparently higher than 2 conventional methods.
10 traditional liquid strain mycelium morphology factor of embodiment is compared with fluid present invention culture medium B mycelium morphology factors
Traditional liquid Spawn incubation based formulas 1 (being denoted as biography 1)：Potato 20.0%, potassium dihydrogen phosphate 0.3%g, magnesium sulfate
0.15%g, sucrose 2.5%；
Traditional liquid Spawn incubation based formulas 2 (being denoted as biography 2)：Potato 20.0%, corn flour 8.0%, potassium dihydrogen phosphate
0.3%g, magnesium sulfate 0.15%g, 2.5% percarbonic acid calcium 1% of sucrose, land plaster 1.0%；
Fluid present invention Spawn incubation based formulas B (is denoted as this)：Potato 5.0%, black Lee discard branch sawdust 10.0%,
Mineral resources humic acid potassium 1.0%, peptone 0.15%, analysis for soybean powder 0.25%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, sucrose
2.5%.
Liquid spawn culture medium is carried out with reference to embodiment 8, and bottle (the triangular glass bottle of 1000ml, every bottle of compost
500ml, sterilizing, then takes parent species to be inoculated with, and culture bottle is cultivated 7 days in 25-28 DEG C of culturing room
The growth of 10 different components fluid nutrient medium mycelia of table is compared
As can be seen from Table 10, the mycelium morphology factor of liquid spawn culture medium B of the present invention has bright than tradition culture
It is aobvious to improve.
Claims (10)
1. a kind of culture medium improving pleurotus edible fungus yield, it is characterised in that with black Lee discard branch sawdust be mainly at
Point.
2. the culture medium according to claim 1 for improving pleurotus edible fungus yield, it is characterised in that contain following component
Potato, black Lee discard branch sawdust, mineral resources humic acid potassium, peptone, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose.
3. the culture medium according to claim 2 for improving pleurotus edible fungus yield, it is characterised in that be culture medium A or training
Base B is supported, composition is respectively：
Culture medium A often discards branch sawdust 100g, mineral resources humic acid in 1000ml culture solutions containing potato 120.0g, black Lee
Potassium 20.0g, peptone 0.5g, analysis for soybean powder 2.5g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-
900ml, is settled to 1000ml, and pH is adjusted to 6.5-7.0；
Culture medium B often discards branch sawdust 100g, mineral resources humic acid potassium in 1000ml culture solutions containing potato 50.0g, black Lee
10.0g, peptone 1.5g, analysis for soybean powder 2.5g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-900ml,
It is settled to 1000ml, pH is adjusted to 6.5-7.0.
4. the culture medium according to claim 3 for improving pleurotus edible fungus yield, it is characterised in that match according to the following steps
System：1. weigh potato by formula, black Lee discards branch sawdust, mineral resources humic acid potassium, peptone, analysis for soybean powder, potassium dihydrogen phosphate,
Magnesium sulfate, sucrose；2. potato slice, black Lee are discarded the particle that branch sawdust crushing is 0.1-0.2cm sizes, with mineral resources corruption
Phytic acid mixes, and adds water 700ml, boils to boiling, and boiling 10-20 minutes, filtering is maintained to remove slag, stay filtered juice；3. the albumen that will be weighed up
Filtrate is added in peptone, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate, sucrose, and dissolving is mixed；4. it adds water in the culture medium of mixing,
Final that culture volume is made to reach 1000ml, pH is adjusted to 6.5-7.0.
5. the culture medium according to claim 1 for improving pleurotus edible fungus yield, it is characterised in that contain following component
Black Lee discards branch sawdust, passion fruit pericarp, decomposed pine soil, mineral resources humic acid potassium, land plaster, calcium superphosphate, lime.
6. the culture medium according to claim 5 for improving pleurotus edible fungus yield, it is characterised in that for culture medium C, training
Base D, culture medium E are supported, composition is respectively：
Culture medium C, per 1000g culture mediums in containing black Lee discard branch sawdust 540g, paulownia waste leaf 150.0g, rice bran 100g,
Passion fruit pericarp 90.0g, decomposed pine soil 50g, peat soil 30g, mineral resources humic acid potassium 10.0g, land plaster 10.0g, peroxophosphoric acid
Calcium 10.0g, lime 10.0g, water 600-700ml；
Culture medium D often discards branch sawdust 500g, paulownia waste leaf 150.0g, passion fruit pericarp in 1000g culture mediums containing black Lee
150.0g, cotton seed hulls 110.0g, decomposed pine soil 50g, mineral resources humic acid potassium 10g, land plaster 10.0g, calcium superphosphate 10.0g,
Lime 10.0g, water 600-700ml；
Culture medium E often discards branch sawdust 600.0g, passion fruit pericarp 150.0g, cotton seed hull in 1000g culture mediums containing black Lee
150.0g, decomposed pine soil 60g, mineral resources humic acid potassium 10g, land plaster 10.0g, calcium superphosphate 10.0g, lime 10.0g, water
600-700ml。
7. the culture medium according to claim 6 for improving pleurotus edible fungus yield, it is characterised in that match according to the following steps
System：1. weighing each ingredient by formula；2. by being mixed except other ingredients of calcium superphosphate mix；3. calcium superphosphate is suitable
It is dissolved in water；4. will 2., 3. mix, and add water in the culture medium of mixing, finally makes moisture content in medium up to 65% or so.
8. the cultural method of the raising pleurotus edible fungus yield using the culture medium of claim 3 or 6, it is characterised in that：
Culture medium A is used for elegant precious mushroom strain cultivation；Or by culture medium B for elm mushroom, oyster mushroom strain cultivation；Or it will training
It supports base C and is used for elegant precious mushroom fruiting bag culture；Or culture medium D is used for elm mushroom fruiting bag culture；Or culture medium E is used for oyster mushroom
Fruiting bag culture.
9. the cultural method according to claim 8 for improving pleurotus edible fungus yield, it is characterised in that：
(1) culture medium A, B are sub-packed in the vial of 1000ml, per bottled culture solution 500ml, 120 DEG C sterilize 40 minutes；Sterilizing
After after culture solution is cooled to room temperature, access 1.0cm × 1.0cm × 1.0cm sizes solid parent species, 25-28 DEG C is shaken at room temperature
Bed culture, shaking table shake fast 115-120 revs/min, and incubation time 7-8 days obtains level liquid strain；
(2) culture medium A, B are sub-packed in the fermentation tank of 300L, per canned culture solution 200L, sterilized 60 minutes at 120 DEG C；Sterilizing
After after culture solution is cooled to room temperature, per tank access 300ml liquid parent species, 25-28 DEG C is cultivated at room temperature；Tank is maintained during culture
0.03-0.05mPa, gas is pressed to build pressure gauge 0.15mPa, incubation time 5-6 days obtains secondary liquid strain；
(3) culture medium C, D, E are sub-packed in 19cm × 40cm polypropylene plastics pockets, per packed siccative 400g, with socket machine nest
After mouthful, sealed with the tampon that chemical fibre cotton is done, sterilization 240 minutes, then take out at 120 DEG C, and bacterium bag, which is cooled to room temperature, to be waited for
With；The bacterium bag of the postcooling that sterilizes to room temperature moves into transfer room inoculation, and 30ml secondary liquid strains, bacterium after inoculation are accessed per culture bag
It is cultivated 15-20 days at room temperature for 22-26 DEG C in bag, obtains the fruiting bag for covering with mycelia.
10. the cultural method according to claim 9 for improving pleurotus edible fungus yield, it is characterised in that fruiting period management
It is carried out by following operation：
Elegant precious mushroom fruiting period management ---
After the long purseful of oyster mushroom pieces, continues to place 5-7 days in culturing room, then transport to mushroom house fruiting；Bacterium bag is used into behind room
Single wall mode is piled up, totally 5 layers, after bacterium bag code is good, opens sack, is beaten cold 2 days with cold is beaten, 10 DEG C of holding therebetween is round the clock
The temperature difference；Beat it is cold after, mushroom house temperature is maintained at 16-25 DEG C, and relative air humidity keeps 85-90%, daily ventilation 2 times, often
Secondary 30 minutes, diffused light is given once daily；Beat it is cold after after 3-5 days on charge level formed mushroom former base, continue holding 85-90% air it is wet
Degree, it is 3 times, every time 30 minutes that ventilation daily, which increases,；When the bacteria cover diameter of elegant precious mushroom reaches 3 centimetres or more, harvesting；
First tide removes fructification residue after adopting, and cuts off the water bacteria after 5 days, then sprays time heavy water, cold flower bud is beaten after 2-3 days, then
Continue that mushroom house temperature is made to be maintained at 16-25 DEG C, air humidity 85%-95%, daily ventilation 2-3 times, every time 30 minutes；Elm mushroom
Management of producing mushroom ---
After the long purseful of mycelia, continue to place 5-7 days in culturing room, moves into mushroom room and carry out management of producing mushroom；Bacterium bag uses single wall
Formula mode is piled up, totally 5 layers, and sack is opened after bacterium bag code is good；Bacterium bag puts mushroom house after a week, and button largely occurs；Mushroom during fruiting
Room temperature is maintained at 18-26 DEG C, air humidity 85%-95%, daily ventilation 2-3 times, 30 minutes every time, elm mushroom from budding to
Harvesting needs 8~10 days；First tide removes fructification residue after adopting, and cuts off the water bacteria after 5 days, then sprays time heavy water, then proceedes to make
Mushroom house temperature is maintained at 18-26 DEG C, air humidity 85%-95%, daily ventilation 2-3 times, every time 30 minutes；
Oyster mushroom fruiting period management ---
After the long purseful of hypha of Pleurotus ostreatus, continue to place 5-7 days in culturing room, moves into mushroom room and carry out management of producing mushroom；Bacterium bag is using single
Row's wall mode is piled up, and totally 6 layers, sack is opened after bacterium bag code is good；Bacterium bag puts mushroom house after a week, and button largely occurs；Fruiting phase
Between mushroom house temperature be maintained at 18-26 DEG C, air humidity 85%-95%, ventilation 2-3 times daily, 30 minutes every time, oyster mushroom was from buddingging
It is needed 8~10 days to harvesting；First tide removes fructification residue after adopting, and cuts off the water bacteria after 5 days, then sprays time heavy water, then proceedes to
Mushroom house temperature is set to be maintained at 18-26 DEG C, air humidity 85%-95%, daily ventilation 2-3 times, every time 30 minutes.
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Cited By (4)
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|---|---|---|---|---|
| CN113079943A (en) * | 2021-04-08 | 2021-07-09 | 徐州工程学院 | Cultivation method and extraction method for increasing content of terpenoid in phellinus igniarius sporocarp |
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