CN1080464A - Simple edible mashroom cultivating method and mother culture media - Google Patents
Simple edible mashroom cultivating method and mother culture media Download PDFInfo
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- CN1080464A CN1080464A CN 92106590 CN92106590A CN1080464A CN 1080464 A CN1080464 A CN 1080464A CN 92106590 CN92106590 CN 92106590 CN 92106590 A CN92106590 A CN 92106590A CN 1080464 A CN1080464 A CN 1080464A
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Abstract
The present invention relates to the cheap mother culture media of a kind of simple edible mashroom cultivating method and this method of being suitable for, belong to the edible mashroom cultivating technical field.Adopted mother culture media that corn grits, wheat bran, flour, ash leachate make to replace traditional mother culture media made from agar, peptone etc.; The atmospheric cooking sterilization is brought out in time-delay; The technical scheme of sterilisation vapour inoculation operation has solved in the room, general house, uses life appliance can't carry out the difficulty of the production of hybrid seeds, and has reduced breeding cost.Have simple and feasible, effective, adapt to wide advantage, especially be fit to specialty cultivation family and use.
Description
The invention discloses the cheap mother culture media of a kind of easy edible mashroom cultivating method and this method of being suitable for, belong to the edible mashroom cultivating technical field.
Make edible fungus species,, require to carry out strict sterilization and sterile working and finish in order to prevent living contaminants.The plant and equipment that this process need is special-purpose aseptic.As high-pressure sterilizing pot, inoculating hood etc.Also need and make the high mother culture media of price that contains agar, peptone etc.Therefore, edible fungus species all is to be produced by the strain plant of specialty, and mostly the cultivation family of being engaged in Edible Fungi is to buy bacterial classification and cultivates.Because the price height of bacterial classification accounts for about 30% of whole cultivation cost, add the inconvenience of purchase and transit link, regular meeting incurs loss through delay the time effects production of cultivation.Especially concerning the specialist in the vast rural area of China, the difficulty that the problems referred to above cause is more outstanding, directly has influence on the economic benefit of Edible Fungi.For the peasant household that edible fungus culturing is engaged in vast rural area, provide a kind of producing method for seed of simple and easy cheapness can fundamentally solve because of buying all inconvenience and the high problem of cost of investment that bacterial classification brings.Increase substantially Edible Fungi and economic benefit.
The purpose of this invention is to provide a kind of simple edible mashroom cultivating method and cheap mother culture media that uses room, general house and life appliance just can finish the edible mashroom cultivating operation.
Technical essential of the present invention shows as:
1, the mother culture media of making in order to corn grits, wheat bran, flour, ash leachate replaces traditional mother culture media made from agar, peptone etc.
2, adopt time-delay to bring out the atmospheric cooking sterilization and replaced traditional instant autoclaving operation.
3, adopt the sterilisation vapour inoculation operation in general people's house to replace traditional aseptic inoculation case inoculation.
The present invention finishes like this:
(1) preparation medium.
1, mother culture media component: corn grits, wheat bran, flour, 3～4% ash leachate.Method: corn grits is put into the ash leachate boil about 15 minutes.Admix wheat bran, become softer dough with hand adjustment, seize a fritter, be twisted into the thick strip of about chopsticks, put into test tube or other containers (as colourless quarter bottle, white medicine bottle etc.), with a thin bamboo plate noodles are flattened, troweling becomes the inclined-plane, and tampon gets final product beyond the Great Wall.
2, iblet pedigree seed culture medium: the corn that selects fresh nothing to go mouldy, soaking the pot of putting into limewash after pulling out in about 36 hours with 0.05～0.1% liquor potassic permanganate boiled 20～30 minutes, make it abundant suction, reach the white heart is arranged slightly, after eliminating with clear water, excessive moisture (or with dried cloth wipe) is removed in cold, take advantage of wet-mixing go into 5% auxiliary material (wherein containing wheat bran, gypsum, lime, ash) mix even, can conventional bottling or pack.
3, wheat straw cultivated species medium is with 100 kilograms of wheat straw or straw (being ground into 0.5～1 centimeter), wheat bran 9%, corn flour 3%, cake fertilizer 3%, lime 2.5%, superphosphate 2%, ash 2%, loam 13%, sugar 0.3%, 160 kilograms in water.
Method: wheat straw, wheat bran, corn flour, cake fertilizer, ash, loam, siccative are mixed even lime is dissolved in sprinkle material in 140 kg of water and mix thoroughly, pile a vexed night, then phosphate fertilizer, sugar are dissolved in 20 kg of water, carefully spill copy in the material even, with the conventional charging of poly-second bag.
(2) the atmospheric cooking sterilization is brought out in time-delay.
Various medium are packed into after the container, and normal temperature was parked 20～50 hours, and again by the local method normal-pressure sterilization, the comparable the original time of sterilization time shortens 50%, and mother culture media can be sterilized 40～60 minutes after the gas of garden in common aluminum pot; The iblet pedigree seed culture medium was sterilized behind the gas of garden 8～12 hours in common steamer; Wheat straw or straw cultivated species medium 4～8 hours.
(3) sterilisation vapour inoculation
At indoor clean one jiao, take 2～4 cubic metres little with Polypropylence Sheet, in little with 3% lysol spraying disinfection.Between in put a stove, seat of honour kettle or little aluminum pot, containers such as big porcelain cylinder are contained the lime clear liquid in the container, water is opened back 8～20 centimeters inoculation above steam in 5～8 minutes.The female kind separated, female tube of planting, and the original seed switching, the single or many people compounding practice of cultivated species switching does not all leave steam.Utensil, personnel's hand disinfection, separation and inoculation method and spawn culture, evaluation, preservation etc. are all carried out routinely.
In sum, the present invention can be summed up as: a kind of simple edible mashroom cultivating method, comprise the making mother culture media, pedigree seed culture medium and cultivated species medium, it is characterized in that above-mentioned three kinds of medium bottling, naturally placed 20～50 hours at normal temperatures after the pack, respectively above-mentioned medium is placed atmospheric cooking sterilization in the ordinary pan then, boiling time is respectively: mother culture media 40～60 minutes; Pedigree seed culture medium 8～12 hours; Cultivated species medium 4～8 hours; Above-mentioned medium container is placed a vessel top that fills the limewash that is seething with excitement, keep suitable distance to carry out mother with boiled water stone and plant and separate, tube, original seed are transferred or the cultivated species switch over operation.Be increase trend according to reducing with temperature the natural standing time of the variation medium of environmental temperature; The concentration of limewash is advisable with 3～5%; According to the aperture area difference of the vessel that fill the limewash that is seething with excitement, in 8～20 centimetres, select with the boiling water surface distance.A kind of simple edible mashroom cultivating, mother culture media is characterized in that being made of corn grits, wheat bran, stone flour, ash leachate, and its mass ratio is a corn grits 3.5～4, wheat bran 1.5～1.8, flour 1～1.2, ash leachate (3～5%) 5～6.5.
Mechanism of the present invention may be summarized to be:
1, according to of the requirement of edible mycelia production phase to nutritional condition, the medium of preparation, mycelium germination is fast, and it is vigorous to grow, and dense sturdy, vitality is strong.Particularly do not have the agar mother culture media, not only saved expensive agar, simplified the operation of irritating pipe and putting the inclined-plane, and mycelial growth is vigorous than agar medium, played the effect of rejuvenation bacterial classification, not only suitable flat mushroom, also more suitable to other edible mushrooms such as woodear, Asparagus.Its mycelium stretches close the knot in the base, has not only enlarged the switching rate, has prolonged the bacterial classification storage time simultaneously, has reduced cost significantly.
2, to bring out atmospheric cooking sterilization be a simple and easy sterilizing methods completely in time-delay.Conventional sterilization (comprising autoclaving) also is difficult to the whole assorted bacterium spores in the composts or fertilisers of cultivating are killed, and exhausts sterilizing methods, though better; but the duration is longer, promptly keeps truce in 1～2 hour after 100 ℃, keep 25～30 ℃ 24 hours; sterilization more totally three times, comparatively bothers.Bring out the time that sterilization was then sterilized 2～3 days evening, impel assorted bacterium spore germination to become trophosome, carry out disposable continuous sterilization again, can kill the assorted bacterium in the medium completely, method is simple, shortening time, fuel saving.
3, the steam that the back generation is opened in sterilisation vapour inoculation, water is aseptic, and the air-flow top that arranges the surrounding air ascension forms aseptic area.But steam does not have bactericidal action, has the air that has assorted bacterium to sneak into unavoidably, adds cheap lime as bactericide in water, has then improved the success rate of inoculation greatly.
Major advantage of the present invention is simple, and cost is low, and the efficient height of the production of hybrid seeds is particularly suitable for the individual production of wide farmers and adopts.For universal seeding technique, solve traditional cultivation and seeding technique one in the poor household of poverty-stricken area and promote difficult problem, to enhance productivity, social benefit has very important effect.It below is the performance comparison table of the present invention and conventional art.
Following discloses two specific embodiments of the present invention.
Embodiment 1: present embodiment is fit to the production of hybrid seeds of flat mushroom.Get corn grits 1000 grams, wheat bran 400 grams, flour 250 grams, ash leachate (3%) 1500 is restrained into mother culture media, places 48 hours naturally, puts into pot boiling sterilization in 40 minutes.The pot top of filling with concentration 3% boiling stone buck that mother culture media is positioned over 30 centimetres is inoculated apart from the water surface 8 centimeters.
Embodiment 2: present embodiment is fit to the production of hybrid seeds of woodear.Get corn grits 1000 grams, wheat bran 350 grams.Flour 250 grams, ash leachate (5%) 1500 is restrained into mother culture media, places 50 hours naturally, puts into pot boiling sterilization in 60 minutes.The pot top of filling with concentration 5% boiling stone buck that mother culture media is positioned over 50 centimetres is inoculated apart from the water surface 10 centimeters.
Claims (4)
1, a kind of simple edible mashroom cultivating method, comprise the making mother culture media, pedigree seed culture medium and cultivated species medium, it is characterized in that and to place 20～50 hours naturally at normal temperatures after above-mentioned three kinds of medium bottling, the pack, respectively above-mentioned medium is placed atmospheric cooking sterilization in the ordinary pan then, boiling time is respectively: mother culture media 40～60 minutes; Pedigree seed culture medium 8～12 hours; Cultivated species medium 4～8 hours; Above-mentioned medium container is placed a vessel top that fills the limewash that is seething with excitement, with boiled water stone keep suitable distance carry out mother plant separate, tube, original seed transfer or the cultivated species switch over operation.
2, according to the described simple edible mashroom cultivating method of claim 1, the concentration that it is characterized in that limewash is advisable with 3～5%, according to the aperture area difference of the vessel that fill the limewash that is seething with excitement, the separation of female kind, tube, original seed switching or the operation of cultivated species transferred species are selected in 8～20 centimetres apart from the distance of the water surface.
3, a kind of simple edible mashroom cultivating mother culture media is characterized in that being made of corn grits, wheat bran flour, ash leachate.
4, according to the described simple edible mashroom cultivating mother culture media of claim 3, it is characterized in that the mass ratio of each component is: corn grits 3.5～4, wheat bran 1.3～1.8, flour 1～1.2, ash leachate (3～5%) 5～6.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92106590 CN1080464A (en) | 1992-07-03 | 1992-07-03 | Simple edible mashroom cultivating method and mother culture media |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 92106590 CN1080464A (en) | 1992-07-03 | 1992-07-03 | Simple edible mashroom cultivating method and mother culture media |
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| CN1080464A true CN1080464A (en) | 1994-01-12 |
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| CN 92106590 Pending CN1080464A (en) | 1992-07-03 | 1992-07-03 | Simple edible mashroom cultivating method and mother culture media |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100364379C (en) * | 2004-08-27 | 2008-01-30 | 李德高 | Edible fungus wood slag mother seed and its making method |
| CN102630485A (en) * | 2012-04-16 | 2012-08-15 | 何寒 | Method for cultivating flammulina velutipes through taking water-retaining agent solidified nutrient solution as culture medium |
| CN103340098A (en) * | 2013-07-19 | 2013-10-09 | 何寒 | Method for preparing edible mushroom test tube mother culture through corn |
| CN103396218A (en) * | 2013-07-17 | 2013-11-20 | 邬金飞 | Formula of Pleurotus citrinopileatus stock compost and preparation method of compost |
| CN109362487A (en) * | 2018-12-12 | 2019-02-22 | 吕梁学院 | A kind of oyster cap fungus culture medium and preparation method thereof |
| CN110463512A (en) * | 2019-09-03 | 2019-11-19 | 贵州大学 | A kind of culture medium combination and its application tamed and produced for oyster mushroom |
-
1992
- 1992-07-03 CN CN 92106590 patent/CN1080464A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100364379C (en) * | 2004-08-27 | 2008-01-30 | 李德高 | Edible fungus wood slag mother seed and its making method |
| CN102630485A (en) * | 2012-04-16 | 2012-08-15 | 何寒 | Method for cultivating flammulina velutipes through taking water-retaining agent solidified nutrient solution as culture medium |
| CN103396218A (en) * | 2013-07-17 | 2013-11-20 | 邬金飞 | Formula of Pleurotus citrinopileatus stock compost and preparation method of compost |
| CN103340098A (en) * | 2013-07-19 | 2013-10-09 | 何寒 | Method for preparing edible mushroom test tube mother culture through corn |
| CN109362487A (en) * | 2018-12-12 | 2019-02-22 | 吕梁学院 | A kind of oyster cap fungus culture medium and preparation method thereof |
| CN110463512A (en) * | 2019-09-03 | 2019-11-19 | 贵州大学 | A kind of culture medium combination and its application tamed and produced for oyster mushroom |
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