CN110073897A - A kind of hypsizigus marmoreus in factory cultivation strain and its cultural method - Google Patents
A kind of hypsizigus marmoreus in factory cultivation strain and its cultural method Download PDFInfo
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- CN110073897A CN110073897A CN201910475037.2A CN201910475037A CN110073897A CN 110073897 A CN110073897 A CN 110073897A CN 201910475037 A CN201910475037 A CN 201910475037A CN 110073897 A CN110073897 A CN 110073897A
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- 238000000034 method Methods 0.000 title claims abstract description 43
- 241000222351 Pleurotus cornucopiae Species 0.000 claims abstract description 51
- 239000000463 material Substances 0.000 claims abstract description 25
- 230000001954 sterilising effect Effects 0.000 claims abstract description 25
- 241000894006 Bacteria Species 0.000 claims abstract description 24
- 238000001816 cooling Methods 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 15
- 235000009566 rice Nutrition 0.000 claims abstract description 15
- 235000013312 flour Nutrition 0.000 claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 241000894007 species Species 0.000 claims abstract description 13
- 238000003306 harvesting Methods 0.000 claims abstract description 11
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 10
- 240000008042 Zea mays Species 0.000 claims abstract description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 10
- 235000005822 corn Nutrition 0.000 claims abstract description 10
- 230000035784 germination Effects 0.000 claims abstract description 10
- 229920000742 Cotton Polymers 0.000 claims abstract description 8
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 5
- 240000007594 Oryza sativa Species 0.000 claims abstract 4
- 239000002609 medium Substances 0.000 claims description 20
- 238000012258 culturing Methods 0.000 claims description 17
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- 238000004659 sterilization and disinfection Methods 0.000 claims description 16
- 238000011081 inoculation Methods 0.000 claims description 13
- 239000012530 fluid Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 230000000638 stimulation Effects 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 10
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 8
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 8
- 239000001965 potato dextrose agar Substances 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 6
- 239000010977 jade Substances 0.000 claims description 5
- 108700022487 rRNA Genes Proteins 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 230000032683 aging Effects 0.000 claims description 4
- 239000002518 antifoaming agent Substances 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000004151 fermentation Effects 0.000 claims description 4
- 230000002070 germicidal effect Effects 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- 230000014759 maintenance of location Effects 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 238000007747 plating Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 230000003519 ventilatory effect Effects 0.000 claims description 4
- 239000002361 compost Substances 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 29
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000035764 nutrition Effects 0.000 abstract description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 2
- 235000019750 Crude protein Nutrition 0.000 abstract description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 abstract description 2
- 239000011575 calcium Substances 0.000 abstract description 2
- 229910052791 calcium Inorganic materials 0.000 abstract description 2
- 150000001720 carbohydrates Chemical class 0.000 abstract description 2
- 239000003818 cinder Substances 0.000 abstract description 2
- 235000019784 crude fat Nutrition 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 239000000835 fiber Substances 0.000 abstract description 2
- 229910052742 iron Inorganic materials 0.000 abstract description 2
- 239000011574 phosphorus Substances 0.000 abstract description 2
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 2
- 239000011591 potassium Substances 0.000 abstract description 2
- 229910052700 potassium Inorganic materials 0.000 abstract description 2
- 239000011734 sodium Substances 0.000 abstract description 2
- 229910052708 sodium Inorganic materials 0.000 abstract description 2
- 239000011701 zinc Substances 0.000 abstract description 2
- 229910052725 zinc Inorganic materials 0.000 abstract description 2
- 241001534815 Hypsizygus marmoreus Species 0.000 description 14
- 241000209094 Oryza Species 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- 241000726811 Carpinus betulus Species 0.000 description 2
- 241001269238 Data Species 0.000 description 2
- 240000001462 Pleurotus ostreatus Species 0.000 description 2
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000014102 seafood Nutrition 0.000 description 2
- 241000589220 Acetobacter Species 0.000 description 1
- 241000609240 Ambelania acida Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 240000005856 Lyophyllum decastes Species 0.000 description 1
- 235000013194 Lyophyllum decastes Nutrition 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000002686 mushroom body Anatomy 0.000 description 1
- 230000027870 phototropism Effects 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A kind of hypsizigus marmoreus in factory cultivation strain and its cultural method, hypsizigus marmoreus in factory cultivation strain are true pleurotus cornucopiae HS-XW08, deposit number are as follows: CGMCC No.14541；A kind of cultural method of hypsizigus marmoreus in factory cultivation strain be cultivated using true pleurotus cornucopiae HS-XW08 as parent species, bacterium germination, management of producing mushroom and harvesting: hypsizigus marmoreus in factory cultivation strain production technology includes that the preparation of culture dish F1 generation strain and culture, triangular flask F2 are prepared and cultivated for liquid spawn, the sterilizing of fermentor stock and cooling, fermentor F3 are prepared and cultivated for strain；Hypsizigus marmoreus in factory cultural method technique, true pleurotus cornucopiae cultivating material formula, corncob, cotton seed hulls, rice bran, wheat bran, corn flour, Bei Huashi, calcium carbonate, water；pH7.0.The true pleurotus cornucopiae of invention production is full of nutrition, and crude protein, crude fat, the true pleurotus cornucopiae fiber of cinder color, carbohydrate, ash content are contained in fresh mushroom, and phosphorus, iron, zinc, calcium, potassium, the content of sodium are high, are rare edible mushrooms, are received by the market.
Description
Technical field
The present invention relates to one of the technology field of edible fungus industrial production hypsizigus marmoreus in factory cultivation strains
And its cultural method, the in particular to industrial bottle of edible mushroom plant strain preparation and its cultural method.
Background technique
True pleurotus cornucopiae (Hypsizygus marmoreus) because it has unique crab delicate flavour, therefore has person it is seafood mushroom.
That cultivates at present has light grey and two strains of pure white, and White strain is also known as " white beech mushroom ", " Yulong mushroom ", deep joyous by market
It meets, mostly factory culture.The mushroom is full of nutrition, is a kind of edible mushroom being of great rarity.
The biological nature of true pleurotus cornucopiae: true pleurotus cornucopiae fructification is grown thickly, and every clump 15-50 plants are differed, sometimes scattered, quantity when scattered
Less and cap is big.It is hemispherical when cap children, it is gradually open and flat after edge is involute, 4-15 centimetres of diameter, near-white to taupe, in
Centre is with dark marble-like speckle.Lamella near-white, with stem at round end shape growing straight, intensively to slightly dilute.Stem is 3-10 lis long
Rice, thick 0.3-0.6 centimetres, wilfully or middle life.Spore is wealthy oval to subsphaeroidal, transparent under microscope, white when in heaps.Mycelia hair
Educating temperature range is 9-30 DEG C, 22-24 DEG C of thermophilic；Fruit body primordium breaks up at 4-18 DEG C, grows 10-14 DEG C of thermophilic；Compost
Water content is advisable 65% or so, keeps the skin wet before fruiting, makes water content up to 70-75%.Mushroom flower bud idiophase, mushroom house relative humidity
98-100% is advisable.Mushroom body puberty mushroom house relative humidity 90-95%；Vegetative stage does not need light, but the mushroom flower bud idiophase answers
There is dim light stimulation.Sporophore growth has phototropism, such as cultivates in basement or cavern, every to open round the clock fluorescent lamp 10-15 hours；
Each stage of true pleurotus cornucopiae growth needs fresh air, should divulge information 4-8 times per hour when especially former base largely occurs；Mycelia growth
The optimum pH 6.5-7.5 in stage.True pleurotus cornucopiae is the edible gill fungus bacterium that north temperate zone fall and winter occurs.
The developmental characteristic of true pleurotus cornucopiae: the white mycelium of true pleurotus cornucopiae, cotton wool shape, aerial hyphae is not vigorous, does not secrete yellow
Drop does not form mycoderma.It can produce arthrospore and chlamydospore in culture journey.True pleurotus cornucopiae resistance is stronger, living condition
There are many something in commons with other domestomycetes such as mushroom, oyster mushroom, but also have difference, and the different strains of true pleurotus cornucopiae are to each
The demand of kind living condition is not exactly the same.True pleurotus cornucopiae is a kind of domestomycetes of low form in nutrition, and culturing raw material is wider, such as
Sawdust, corncob, bagasse and cotton seed hulls etc. all can be used as primary raw material, with the yield highest of cotton seed hulls.In cultivation
In plus a small amount of auxiliary material, such as per unit area yield can be improved in rice bran, wheat bran, soybean skin, cottonseed cake and corn flour.In temperature true pleurotus cornucopiae with
There are alternating temperature Grain-Filling Characteristics as needle mushroom, sliding mushroom, mushroom and oyster mushroom etc..True pleurotus cornucopiae bacterium germination needs 30-45 days, and mycelia maturation reaches
There is fruiting ability to take 30-60 days, the differentiation of mushroom flower bud needs 7-12 days, and fruit body development is at being about 5-7 days.Time control should be according to difference
Strain and temperature are suitably flexible.Careful observation controls in time, is the key that cultivation success or failure and quality height.
The patent No. 201710213080.2 discloses a kind of true pleurotus cornucopiae cultural method, the cultural method the problem is that:
Culture material contains 35 parts of sawdust, and present the Northeast's sawdust amount is few, and price is high；The bacterium germination time is 120 days, and the production cycle is long；Gu
The body kind production cycle is long.
The patent No. 201810011228.9 discloses a kind of true pleurotus cornucopiae bacterial strain and its cultural method, the bacterial strain and its culture side
Method the problem is that: solid spawn, the production cycle is long, 70-78 days.
The patent No. 201810530728.3 discloses a kind of double-colored true pleurotus cornucopiae cultural method, the cultural method there are the problem of
It is that culture material contains 48.1% sawdust, and present the Northeast's sawdust amount is few, price is high；The solid spawn production cycle is long.
The cultural method of true pleurotus cornucopiae in the prior art: nutrition, culture material formula, inoculation bacterium germination, temperature, moisture, light,
Air, pH value, management of producing mushroom etc. there is also some problems.Production cycle is long, and product price is high.Therefore, one is developed
Kind hypsizigus marmoreus in factory cultivation strain and its cultural method are always project anxious to be resolved.
Summary of the invention
The purpose of the present invention is to provide a kind of hypsizigus marmoreus in factory cultivation strain and its cultural method, asked existing for solution
Topic is to realize that saprophytic bacteria carries out factory culture, the strain of true pleurotus cornucopiae production is planted with industrial bottle, for true after artificial culture
The factorial production of pleurotus cornucopiae.
True pleurotus cornucopiae (Hypsizygus marmoreus) HS-XW08 of the invention, is deposited on July 27th, 2017
China Committee for Culture Collection of Microorganisms's common micro-organisms center, abbreviation CGMCC, address are Chaoyang District, Beijing City North Star west
The institute 3 of road 1.Deposit number are as follows: CGMCC No.14541.
Culture of true pleurotus cornucopiae (Hypsizygus marmoreus) HS-XW08 through institute of microbiology, the Chinese Academy of Sciences of the invention
The experimental datas comprehensive analysis such as feature, microscopic features and rRNA gene order confirm that the qualification result of HS-XW08 is
The true pleurotus cornucopiae of Hypsizygus marmoreus.
The object of the present invention is achieved like this: a kind of hypsizigus marmoreus in factory cultivation strain, the hypsizigus marmoreus in factory
Cultivation strain is true pleurotus cornucopiae HS-XW08, deposit number are as follows: CGMCC No.14541；
A kind of rRNA gene order of hypsizigus marmoreus in factory cultivation strain is:
3’-AGAGTTGTCCCGTGAAGGGGACGGTTAGAAGCTGAACCCATAATTAAAGCTGCTTCGCAATGGCGTAGA
TAATTATCACACCAGAGCTGGTCAACAAAGGTTCCGCTAATGCATTTAAGGAGAGCCGACTTCTGAGAAGCCCGCA
ACCCCCACATCCAAGCCTGACCAAGCTAnTAAAAGCTGGAAAGGTTGAGAATTTAATGACACTCAAACAGGCATGC
TCCTCGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGCAATTCACATTACTTA
TCGCATTTCGCTGCGCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTGTATTTGATTTAA
AGGCACTAAGGCCCATTAAAGACATTCAGTTACATTCATGGGGTATATAAAGACATAGACCGGGAACGCAAGGAAA
GCCGGCTTTTCAGCAGCGGCAATCCTCAAACCGAGTTACCTCGAGAGGTGTTCCAGGTCTACAAAGGGTGCACAGG
TGGTAAAAATGTGTCAGGCGTGCACATGCTCCTAAGAGCCAGCAACAACCCAAACAAGTTTATTCAATAATGATCC
TTCCGCAGGTTCACCTACGGAAACCTTGTT-5'；
A kind of cultural method of the hypsizigus marmoreus in factory cultivation strain be cultivated using true pleurotus cornucopiae HS-XW08 as parent species,
Bacterium germination, management of producing mushroom and harvesting:
(1) the hypsizigus marmoreus in factory cultivation strain technological process of production:
1. the preparation of culture dish F1 generation strain and culture: it prepares potato dextrose agar (PDA), i.e. solid medium,
121 DEG C of 15min sterilize, and dispense into culture dish, parent species are transferred in plating medium, 20-22 DEG C of cultivation temperature,
Culture 16-18 days；
2. triangular flask F2 is prepared and is cultivated for liquid spawn: preparing fluid nutrient medium, white sugar 20g, beans are added in 1000ml water
Dregs of rice powder 5g, corn flour 4g, yeast powder 2g, magnesium sulfate 1g, potassium dihydrogen phosphate 1g；Fluid nutrient medium is packed into the triangular flask of 1000ml
In, each bottled fluid nutrient medium 600ml of triangle is put into high-pressure sterilizing pot, and 121 DEG C of temperature, the time, 15min sterilized,
The preferable Mother culture ware F1 generation strain of growth conditions is inoculated into triangular flask, is placed on shaking table, 20-22 DEG C of temperature, 120
Rev/min, dark culturing, 10-12 days；
3. fermentor stock, sterilizing and cooling: white sugar 25g, bean cake powder 4g, jade fermentation tank culture liquid: being added in 1000 ml water
Rice flour 3g, magnesium sulfate 1g, potassium dihydrogen phosphate 1g, defoaming agent 0.3ml, natural ph, carry out autoclave sterilization, ventilation water drench to
20-22℃；
4. fermentor F3 is prepared and is cultivated for strain: by F2 for formation F3 in strain transfer to fermentor for strain, temperature 20-22
DEG C, ventilatory capacity is 1.2-1.7 m3/ h is cultivated 8-10 days；
(2) hypsizigus marmoreus in factory cultural method process flow:
1. culture material ingredient, stirring, bottling: when planting industrialized agribusiness using bottle, parent species being cultivated original seed and cultivar, then will
Cultivar is inoculated into bottled culture material；
2. culture bottle entrucking is pushed into Sterilization Kettle and carries out autoclave sterilization by Sterilization Kettle；
3. fore-cooling room, material bottle pre-cooling；
4. cooling chamber, material bottle cooling, gradually cool down, are cooled to 20-22 DEG C；
5. fermentor F3 is seeded in culture bottle, inoculum concentration 30-35g for strain by liquid inoculation device by inoculation device room；
6. the culture bottle after inoculation is transferred to culturing room, carries out dark culturing, be suitably passed through fresh air by culturing room, germicidal management,
Culture 90 days, 22-24 DEG C of temperature, humidity 70-85%, gas concentration lwevel 2500-4500ppm；
7. mycelium stimulation washes bacterium, Jia Shui, after mycelia is covered in culture bottle, carries out mechanical mycelium stimulation and wash bacterium, remove aging bacterium layer；
8. workshop is cultivated, birth management, the period 30 days, 14-16 DEG C of temperature, humidity 85-100%, gas concentration lwevel 2000-
3500ppm；
9. packing shop, harvesting management；
10. being put in storage, 4 DEG C of storages；
The cultural method of the hypsizigus marmoreus in factory cultivation strain, the true pleurotus cornucopiae cultivating material formula is by following each component weight
1000g compost: corncob 140g, cotton seed hulls 35g, rice bran 70g, wheat bran 52.5g, corn flour 35g, Bei Huashi is made in amount proportion
10.5g, calcium carbonate 7g, water 650g； pH7.0.
True pleurotus cornucopiae (Hypsizygus marmoreus) HS-XW08 of the invention has characteristics that
True pleurotus cornucopiae (Hypsizygus marmoreus), also known as beautiful gill fungus, spot jade gill fungus, Lyophyllum decastes.Because it has unique crab
Delicate flavour, thus have person it be crab flavour mushroom, seafood mushroom.
Beneficial effects of the present invention: true pleurotus cornucopiae (Hypsizygus marmoreus) HS-XW08 is full of nutrition, every according to analysis
Contain 3.22 grams of crude protein, 0.22 gram of crude fat, 1.68 grams of the true pleurotus cornucopiae fiber of cinder color, carbohydrate 4.56 in 100 grams of fresh mushrooms
Gram, 1.32 grams of ash content, phosphorus, iron, zinc, calcium, potassium, the content of sodium are very rich, vitamin B1、B2、B6, C content also more general mushroom
Class is high, is a kind of edible mushroom being of great rarity.
Product cultivation of the invention has light grey and two strains of pure white, and White strain is also known as " white beech mushroom ", " Yulong
Mushroom ", it is received by the market.Mostly factory culture.
Detailed description of the invention
The following describes the present invention in detail with reference to the accompanying drawings and embodiments.
Fig. 1 is hypsizigus marmoreus in factory cultivation strain production technological process of the invention.
Fig. 2 is hypsizigus marmoreus in factory cultural method process flow chart of the invention.
Fig. 3 is present invention mycelia situation map under the microscope.
Fig. 4 is triangular flask bacterial culture figure of the invention.
Fig. 5 is 1 culture figure of fermentor strain of the invention.
Fig. 6 is 2 culture figure of fermentor strain of the invention.
Fig. 7 is that bottle of the invention plants 1 situation map of bacterium germination.
Fig. 8 is that bottle of the invention plants 2 situation map of bacterium germination.
Fig. 9 is that bottle of the invention plants fruiting situation map.
Figure 10 is plate Spawn incubation figure of the invention.
Figure 11 is inoculating facility figure of the invention.
Figure 12 is schemed before mycelium stimulation of the invention.
Figure 13 is schemed after mycelium stimulation of the invention.
Figure 14 is the true pleurotus cornucopiae figure of harvesting of the invention.
Figure 15 is the true pleurotus cornucopiae weighing figure of harvesting of the invention.
Specific embodiment
True pleurotus cornucopiae (Hypsizygus marmoreus) HS-XW08 of the invention, is deposited on July 27th, 2017
China Committee for Culture Collection of Microorganisms's common micro-organisms center, abbreviation CGMCC, address are Chaoyang District, Beijing City North Star west
The institute 3 of road 1.Deposit number are as follows: CGMCC No.14541.
Culture of true pleurotus cornucopiae (Hypsizygus marmoreus) HS-XW08 through institute of microbiology, the Chinese Academy of Sciences of the invention
The experimental datas comprehensive analysis such as feature, microscopic features and rRNA gene order confirm that the qualification result of HS-XW08 is
The true pleurotus cornucopiae of Hypsizygus marmoreus.
A kind of hypsizigus marmoreus in factory cultivation strain, the hypsizigus marmoreus in factory cultivation strain are true pleurotus cornucopiae HS-XW08, are protected
Hiding number are as follows: CGMCC No.14541.
The hypsizigus marmoreus in factory cultivation strain, a kind of rRNA gene sequence of hypsizigus marmoreus in factory cultivation strain
Column are:
3’-AGAGTTGTCCCGTGAAGGGGACGGTTAGAAGCTGAACCCATAATTAAAGCTGCTTCGCAATGGCGTAGA
TAATTATCACACCAGAGCTGGTCAACAAAGGTTCCGCTAATGCATTTAAGGAGAGCCGACTTCTGAGAAGCCCGCA
ACCCCCACATCCAAGCCTGACCAAGCTAnTAAAAGCTGGAAAGGTTGAGAATTTAATGACACTCAAACAGGCATGC
TCCTCGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGCAATTCACATTACTTA
TCGCATTTCGCTGCGCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTGTATTTGATTTAA
AGGCACTAAGGCCCATTAAAGACATTCAGTTACATTCATGGGGTATATAAAGACATAGACCGGGAACGCAAGGAAA
GCCGGCTTTTCAGCAGCGGCAATCCTCAAACCGAGTTACCTCGAGAGGTGTTCCAGGTCTACAAAGGGTGCACAGG
TGGTAAAAATGTGTCAGGCGTGCACATGCTCCTAAGAGCCAGCAACAACCCAAACAAGTTTATTCAATAATGATCC
TTCCGCAGGTTCACCTACGGAAACCTTGTT-5’。
Embodiment one
The cultural method of the hypsizigus marmoreus in factory cultivation strain, a kind of cultivation of hypsizigus marmoreus in factory cultivation strain
Method be cultivated using true pleurotus cornucopiae HS-XW08 as parent species, bacterium germination, management of producing mushroom and harvesting:
(1) the hypsizigus marmoreus in factory cultivation strain technological process of production (being detailed in attached drawing 1):
1. the preparation of culture dish F1 generation strain and culture: it prepares potato dextrose agar (PDA), i.e. solid medium,
121 DEG C of 15min sterilize, and dispense into culture dish, parent species (fructification) are transferred in plating medium, cultivation temperature
It 20 DEG C, cultivates 16 days；
2. triangular flask F2 is prepared and is cultivated for liquid spawn: preparing fluid nutrient medium, white sugar 20g, beans are added in 1000ml water
Dregs of rice powder 5g, corn flour 4g, yeast powder 2g, magnesium sulfate 1g, potassium dihydrogen phosphate 1g；Fluid nutrient medium is packed into the triangular flask of 1000ml
In, each bottled fluid nutrient medium 600ml of triangle is put into high-pressure sterilizing pot, and 121 DEG C of temperature, the time, 15min sterilized,
The preferable Mother culture ware F1 generation strain of growth conditions is inoculated into triangular flask, is placed on shaking table, 20 DEG C of temperature, 120 turns/
Minute, dark culturing, 10 days；
3. fermentor stock, sterilizing and cooling: white sugar 25g, bean cake powder 4g, jade fermentation tank culture liquid: being added in 1000 ml water
Rice flour 3g, magnesium sulfate 1g, potassium dihydrogen phosphate 1g, defoaming agent 0.3ml, natural ph, carry out autoclave sterilization, ventilation water drench to
20℃；
4. fermentor F3 is prepared and is cultivated for strain: by F2 for forming F3 in strain transfer to fermentor for strain, 20 DEG C of temperature,
Ventilatory capacity is 1.2 m3/ h is cultivated 8 days；
(2) hypsizigus marmoreus in factory cultural method process flow (being detailed in attached drawing 2):
1. culture material ingredient, stirring, bottling: when planting industrialized agribusiness using bottle, parent species being cultivated original seed and cultivar, then will
Cultivar is inoculated into bottled culture material；True pleurotus cornucopiae cultivating material is prepared: corncob 140g, cotton seed hulls 35g, rice bran 70g, wheat bran
52.5g, corn flour 35g, Bei Huashi 10.5g, calcium carbonate 7g, water 650g； pH7.0.
2. culture bottle entrucking is pushed into Sterilization Kettle and carries out autoclave sterilization by Sterilization Kettle；
3. fore-cooling room, material bottle pre-cooling；
4. cooling chamber, material bottle cooling, gradually cool down, are cooled to 20 DEG C；
5. fermentor F3 is seeded in culture bottle, inoculum concentration 30g for strain by liquid inoculation device by inoculation device room；
6. the culture bottle after inoculation is transferred to culturing room, carries out dark culturing, be suitably passed through fresh air by culturing room, germicidal management,
Culture 90 days, 22 DEG C of temperature, humidity 70%, gas concentration lwevel 2500ppm；
7. mycelium stimulation washes bacterium, Jia Shui, after mycelia is covered in culture bottle, carries out mechanical mycelium stimulation and wash bacterium, remove aging bacterium layer；
8. workshop is cultivated, birth management, the period 30 days, 14 DEG C of temperature, humidity 85%, gas concentration lwevel 2000ppm；
9. packing shop, harvesting management；
10. being put in storage, 4 DEG C of storages.
Embodiment two
The cultural method of the hypsizigus marmoreus in factory cultivation strain, a kind of cultivation of hypsizigus marmoreus in factory cultivation strain
Method be cultivated using true pleurotus cornucopiae HS-XW08 as parent species, bacterium germination, management of producing mushroom and harvesting:
(1) the hypsizigus marmoreus in factory cultivation strain technological process of production (being detailed in attached drawing 1):
1. the preparation of culture dish F1 generation strain and culture: it prepares potato dextrose agar (PDA), i.e. solid medium,
121 DEG C of 15min sterilize, and dispense into culture dish, parent species (fructification) are transferred in plating medium, cultivation temperature
22 DEG C of cultures, 18 days；
2. triangular flask F2 is prepared and is cultivated for liquid spawn: preparing fluid nutrient medium, white sugar 20g, beans are added in 1000ml water
Dregs of rice powder 5g, corn flour 4g, yeast powder 2g, magnesium sulfate 1g, potassium dihydrogen phosphate 1g；Fluid nutrient medium is packed into the triangular flask of 1000ml
In, each bottled fluid nutrient medium 600ml of triangle is put into high-pressure sterilizing pot, and 121 DEG C of temperature, the time, 15min sterilized,
The preferable Mother culture ware F1 generation strain of growth conditions is inoculated into triangular flask, is placed on shaking table, 22 DEG C of temperature, 120 turns/
Minute, dark culturing, 12 days；
3. fermentor stock, sterilizing and cooling: white sugar 25g, bean cake powder 4g, jade fermentation tank culture liquid: being added in 1000 ml water
Rice flour 3g, magnesium sulfate 1g, potassium dihydrogen phosphate 1g, defoaming agent 0.3ml, natural ph, carry out autoclave sterilization, ventilation water drench to
22℃；
4. fermentor F3 is prepared and is cultivated for strain: by F2 for forming F3 in strain transfer to fermentor for strain, 22 DEG C of temperature,
Ventilatory capacity is 1.7 m3/ h is cultivated 10 days；
(2) hypsizigus marmoreus in factory cultural method process flow (being detailed in attached drawing 2):
1. culture material ingredient, stirring, bottling: when planting industrialized agribusiness using bottle, parent species being cultivated original seed and cultivar, then will
Cultivar is inoculated into bottled culture material；True pleurotus cornucopiae cultivating material is prepared: corncob 140g, cotton seed hulls 35g, rice bran 70g, wheat bran
52.5g, corn flour 35g, Bei Huashi 10.5g, calcium carbonate 7g, water 650g； pH7.0.
2. culture bottle entrucking is pushed into Sterilization Kettle and carries out autoclave sterilization by Sterilization Kettle；
3. fore-cooling room, material bottle pre-cooling；
4. cooling chamber, material bottle cooling, gradually cool down, are cooled to 22 DEG C；
5. fermentor F3 is seeded in culture bottle, inoculum concentration 35g for strain by liquid inoculation device by inoculation device room；
6. the culture bottle after inoculation is transferred to culturing room, carries out dark culturing, be suitably passed through fresh air by culturing room, germicidal management,
Culture 90 days, 24 DEG C of temperature, humidity 85%, gas concentration lwevel 4500ppm；
7. mycelium stimulation washes bacterium, Jia Shui, after mycelia is covered in culture bottle, carries out mechanical mycelium stimulation and wash bacterium, remove aging bacterium layer；
8. workshop is cultivated, birth management, the period 30 days, 16 DEG C of temperature, humidity 100%, gas concentration lwevel 3500ppm；
9. packing shop, harvesting management；
10. being put in storage, 4 DEG C of storages.
Sequence table
<110>agricultural development Co., Ltd, Shenyang Hang Seng, biotechnology Development Co., Ltd, Shenyang Hang Seng
<120>a kind of hypsizigus marmoreus in factory cultivation strain and its cultural method
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 43
<212> DNA
<213>true pleurotus cornucopiae (Hypsizygus marmoreus)
<400> 1
ttgttccaaa ggcatccact tggacgcctt cctagtaata act 43
<210> 2
<211> 229
<212> DNA
<213>true pleurotus cornucopiae (Hypsizygus marmoreus)
<400> 2
tatttgaaca aacccaacaa cgaccgagaa tcctcgtaca cgtgcggact gtgtaaaaat 60
ggtggacacg tgggaaacat ctggaccttg tggagagctc cattgagcca aactcctaac 120
ggcgacgact tttcggccga aaggaacgca agggccagat acagaaatat atggggtact 180
tacattgact tacagaaatt acccggaatc acggaaattt agtttatgt 229
<210> 3
<211> 168
<212> DNA
<213>true pleurotus cornucopiae (Hypsizygus marmoreus)
<400> 3
tgaaagttgt tgcctagaga accgagagcg tagctacttc ttgcgtcgcg tcgctttacg 60
ctattcatta cacttaacgt cttaagtcac ttagtagctt agaaacttgc gtggaacgcg 120
aggaaccata aggctcctcg tacggacaaa ctcacagtaa tttaagag 168
<210> 4
<211> 191
<212> DNA
<213>true pleurotus cornucopiae (Hypsizygus marmoreus)
<400> 4
ttggaaaggt cgaaaatnat cgaaccagtc cgaacctaca cccccaacgc ccgaagagtc 60
ttcagccgag aggaatttac gtaatcgcct tggaaacaac tggtcgagac cacactatta 120
atagatgcgg taacgcttcg tcgaaattaa tacccaagtc gaagattggc aggggaagtg 180
ccctgttgag a 191
Claims (4)
1. a kind of hypsizigus marmoreus in factory cultivation strain, it is characterised in that: the hypsizigus marmoreus in factory cultivation strain is true pleurotus cornucopiae
HS-XW08, deposit number are as follows: CGMCC No.14541.
2. a kind of hypsizigus marmoreus in factory cultivation strain as described in claim 1, it is characterised in that: a kind of true pleurotus cornucopiae work
The rRNA gene order of factory's cultivation strain is:
3’-AGAGTTGTCCCGTGAAGGGGACGGTTAGAAGCTGAACCCATAATTAAAGCTGCTTCGCAATGGCGTAGA
TAATTATCACACCAGAGCTGGTCAACAAAGGTTCCGCTAATGCATTTAAGGAGAGCCGACTTCTGAGAAGCCCGCA
ACCCCCACATCCAAGCCTGACCAAGCTAnTAAAAGCTGGAAAGGTTGAGAATTTAATGACACTCAAACAGGCATGC
TCCTCGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGCAATTCACATTACTTA
TCGCATTTCGCTGCGCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTGTATTTGATTTAA
AGGCACTAAGGCCCATTAAAGACATTCAGTTACATTCATGGGGTATATAAAGACATAGACCGGGAACGCAAGGAAA
GCCGGCTTTTCAGCAGCGGCAATCCTCAAACCGAGTTACCTCGAGAGGTGTTCCAGGTCTACAAAGGGTGCACAGG
TGGTAAAAATGTGTCAGGCGTGCACATGCTCCTAAGAGCCAGCAACAACCCAAACAAGTTTATTCAATAATGATCC
TTCCGCAGGTTCACCTACGGAAACCTTGTT-5’。
3. a kind of cultural method of hypsizigus marmoreus in factory cultivation strain as claimed in claim 1 or 2, it is characterised in that: described
A kind of hypsizigus marmoreus in factory cultivation strain cultural method be cultivated using true pleurotus cornucopiae HS-XW08 as parent species, bacterium germination, fruiting
It manages and harvests:
(1) the hypsizigus marmoreus in factory cultivation strain technological process of production:
1. the preparation of culture dish F1 generation strain and culture: it prepares potato dextrose agar (PDA), i.e. solid medium,
121 DEG C of 15min sterilize, and dispense into culture dish, parent species are transferred in plating medium, 20-22 DEG C of cultivation temperature,
Culture 16-18 days；
2. triangular flask F2 is prepared and is cultivated for liquid spawn: preparing fluid nutrient medium, white sugar 20g, beans are added in 1000ml water
Dregs of rice powder 5g, corn flour 4g, yeast powder 2g, magnesium sulfate 1g, potassium dihydrogen phosphate 1g；Fluid nutrient medium is packed into the triangular flask of 1000ml
In, each bottled fluid nutrient medium 600ml of triangle is put into high-pressure sterilizing pot, and 121 DEG C of temperature, the time, 15min sterilized,
The preferable Mother culture ware F1 generation strain of growth conditions is inoculated into triangular flask, is placed on shaking table, 20-22 DEG C of temperature, 120
Rev/min, dark culturing, 10-12 days；
3. fermentor stock, sterilizing and cooling: white sugar 25g, bean cake powder 4g, jade fermentation tank culture liquid: being added in 1000 ml water
Rice flour 3g, magnesium sulfate 1g, potassium dihydrogen phosphate 1g, defoaming agent 0.3ml, natural ph, carry out autoclave sterilization, ventilation water drench to
20-22℃；
4. fermentor F3 is prepared and is cultivated for strain: by F2 for formation F3 in strain transfer to fermentor for strain, temperature 20-22
DEG C, ventilatory capacity is 1.2-1.7 m3/ h is cultivated 8-10 days；
(2) hypsizigus marmoreus in factory cultural method process flow:
1. culture material ingredient, stirring, bottling: when planting industrialized agribusiness using bottle, parent species being cultivated original seed and cultivar, then will
Cultivar is inoculated into bottled culture material；
2. culture bottle entrucking is pushed into Sterilization Kettle and carries out autoclave sterilization by Sterilization Kettle；
3. fore-cooling room, material bottle pre-cooling；
4. cooling chamber, material bottle cooling, gradually cool down, are cooled to 20-22 DEG C；
5. fermentor F3 is seeded in culture bottle, inoculum concentration 30-35g for strain by liquid inoculation device by inoculation device room；
6. the culture bottle after inoculation is transferred to culturing room, carries out dark culturing, be suitably passed through fresh air by culturing room, germicidal management,
Culture 90 days, 22-24 DEG C of temperature, humidity 70-85%, gas concentration lwevel 2500-4500ppm；
7. mycelium stimulation washes bacterium, Jia Shui, after mycelia is covered in culture bottle, carries out mechanical mycelium stimulation and wash bacterium, remove aging bacterium layer；
8. workshop is cultivated, birth management, the period 30 days, 14-16 DEG C of temperature, humidity 85-100%, gas concentration lwevel 2000-
3500ppm；
9. packing shop, harvesting management；
10. being put in storage, 4 DEG C of storages.
4. a kind of cultural method of the hypsizigus marmoreus in factory cultivation strain as described in claims 1 or 2 or 3, it is characterised in that: institute
The true pleurotus cornucopiae cultivating material formula stated is matched by following composition weight and 1000g compost: corncob 140g is made, cotton seed hulls 35g,
Rice bran 70g, wheat bran 52.5g, corn flour 35g, Bei Huashi 10.5g, calcium carbonate 7g, water 650g； pH7.0.
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| CN110521500A (en) * | 2019-10-10 | 2019-12-03 | 西充星河生物科技有限公司 | A kind of true pleurotus cornucopiae liquid spawn production technology |
| CN110583368A (en) * | 2019-10-10 | 2019-12-20 | 西充星河生物科技有限公司 | Hypsizigus marmoreus culture medium and application method thereof |
| CN111480514A (en) * | 2020-05-08 | 2020-08-04 | 江苏华绿生物科技股份有限公司 | Culture medium for industrial cultivation of hypsizigus marmoreus |
| CN111527992A (en) * | 2020-05-06 | 2020-08-14 | 江苏华绿生物科技股份有限公司 | Culture medium for hypsizigus marmoreus |
| CN112840962A (en) * | 2021-04-14 | 2021-05-28 | 江苏海洋大学 | Preparation method of Hypsizygus marmoreus liquid strain |
| CN113637591A (en) * | 2020-04-27 | 2021-11-12 | 西充星河生物科技有限公司 | Hypsizigus marmoreus strain |
| CN113728876A (en) * | 2021-10-11 | 2021-12-03 | 韶关市星河生物科技有限公司 | Industrialized solid strain production process of hypsizigus marmoreus |
| CN114804957A (en) * | 2022-05-13 | 2022-07-29 | 山东常生源生物科技股份有限公司 | Industrial selenium-rich disease-resistant cultivation method for hypsizigus marmoreus and nutrient solution used by method |
| CN115956481A (en) * | 2023-02-10 | 2023-04-14 | 福建省农业科学院农业工程技术研究所 | Hypsizigus marmoreus capable of being rehydrated quickly and preparation method thereof |
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| CN110521500A (en) * | 2019-10-10 | 2019-12-03 | 西充星河生物科技有限公司 | A kind of true pleurotus cornucopiae liquid spawn production technology |
| CN110583368A (en) * | 2019-10-10 | 2019-12-20 | 西充星河生物科技有限公司 | Hypsizigus marmoreus culture medium and application method thereof |
| CN113637591A (en) * | 2020-04-27 | 2021-11-12 | 西充星河生物科技有限公司 | Hypsizigus marmoreus strain |
| CN113637591B (en) * | 2020-04-27 | 2023-10-03 | 西充星河生物科技有限公司 | Hypsizygus marmoreus strain |
| CN111527992A (en) * | 2020-05-06 | 2020-08-14 | 江苏华绿生物科技股份有限公司 | Culture medium for hypsizigus marmoreus |
| CN111480514A (en) * | 2020-05-08 | 2020-08-04 | 江苏华绿生物科技股份有限公司 | Culture medium for industrial cultivation of hypsizigus marmoreus |
| CN112840962A (en) * | 2021-04-14 | 2021-05-28 | 江苏海洋大学 | Preparation method of Hypsizygus marmoreus liquid strain |
| CN113728876A (en) * | 2021-10-11 | 2021-12-03 | 韶关市星河生物科技有限公司 | Industrialized solid strain production process of hypsizigus marmoreus |
| CN114804957A (en) * | 2022-05-13 | 2022-07-29 | 山东常生源生物科技股份有限公司 | Industrial selenium-rich disease-resistant cultivation method for hypsizigus marmoreus and nutrient solution used by method |
| CN115956481A (en) * | 2023-02-10 | 2023-04-14 | 福建省农业科学院农业工程技术研究所 | Hypsizigus marmoreus capable of being rehydrated quickly and preparation method thereof |
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