CN109220529A - A kind of Bacillus cercus and its application in promotion edible fungi growth - Google Patents
A kind of Bacillus cercus and its application in promotion edible fungi growth Download PDFInfo
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Abstract
The present invention provides a kind of Bacillus cercus and its promoting the application in edible fungi growth, the Bacillus cercus is named as Bac1, can effectively facilitate mycelial growth rate, improves mushroom yield, and effectively shorten its growth cycle, and then increase the economic benefit of Edible Fungi.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to a kind of Bacillus cercus and its in promoting edible fungi growth
Application.
Background technique
Edible mushroom delicious flavour, unique flavor, mouthfeel are smooth, and rich in necessary to protein, cellulose and human body
A variety of amino acid, and a variety of mineral nutrient elements such as can provide phosphorus, calcium, iron, being recognized in the world is optimal egg
White matter and nutrient combination source.But much the edible fungi growth time is longer, vulnerable to living contaminants, and then affects its yield.
Bacillus cercus (Bacillus cereus) is the endophyte of some cereal crops, vegetables and trees, can planted
Object is in vivo colonized, and does not generate any harm to host plant, can promote plant growth, reduces pest and disease damage, is improved plant and is resisted
The ability of poor environment.But Bacillus cercus is promoting the application in edible fungi growth and is having not been reported.
Summary of the invention
For the above-mentioned problems in the prior art, the present invention provide a kind of Bacillus cercus and its promote it is edible
Application in bacterium growth is especially promoting the mycelia growing of pleurotus eryngii, the developmental application of sporophore growth.
To achieve the above object, the technical solution adopted by the present invention to solve the technical problems is:
The present invention is by randomly selecting the Pleurotus eryngii bacterium bag in Pleurotus eryngii mushroom factory, to the Pleurotus eryngii bacterium bag of different growing stage
In bacterium separated, purified, obtain a kind of bacterial strain that can promote edible mushroom especially Growth of Pleurotus eryngii, be by the Strain Designation
Bac1.The bacterial strain is accredited as Bacillus cercus through microbiology.On LB plating medium, for 24 hours in 37 DEG C of cultures, circle is formed
Shape is approximate circle, glossiness white colony, bacterium colony rough surface, it is flat, be in irregular status；It observes under the microscope,
Cell is in the shape of a rod, and can produce gemma, and gemma is oval；It is positive strain through Gram's staining.According to the 9th edition " primary Jie Shi system
System bacteriology handbook " and " common bacteria system identification handbook " carries out morphology measurement to Bac1 bacterial strain, Physiology and biochemistry detects,
The analysis of 16S rRNA sequencing and phylogenetic tree determines that Bac1 bacterial strain is the member in Bacillus cercus.
Bacillus cercus Bac1 was preserved in Chinese microorganism strain preservation conservator on September 17th, 2018
Meeting common micro-organisms center, deposit number are CGMCC No.16475, and preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
No. 3, Institute of Microorganism, Academia Sinica bacterium protects center.
Bacillus cercus Bac1 is promoting in edible fungi growth in application, the time is added are as follows: before culture material sterilizing
It is added and is either added in the edible mushroom especially the mycelia growing of pleurotus eryngii stage, preferably grow to bacterium bottle or bacterium bag 1/ in mycelia
It is added when at 2.
Further, Bacillus cercus Bac1 is added in culture material in a manner of fermentation liquid.
Further, the additive amount of Bacillus cercus Bac1 is the 5%~10% of culture material weight.
The present invention also provides a kind of edible mushroom promotor, which includes Bacillus cercus Bac1, is planted in edible mushroom
The edible mushroom promotor is added during training, can promote hypha of edible fungus growth and the growth of fructification, to increase mushroom body
Weight shortens planting time.
Bacillus cercus provided by the invention and its application in promotion edible fungi growth, have below beneficial to effect
Fruit:
The Bacillus cercus Bac1 that the present invention is separated to can promote hypha of edible fungus growth, especially Pleurotus eryngii mycelia
Growth studies it by different processing modes (the fermentation liquid situation of Bacillus cercus Bac1, addition time, additional amount)
Influence to edible mushroom especially Growth of Pleurotus eryngii, main research are as follows: straight to mycelial growth rate, mycelia on PDA plate
The measurement of diameter, the measurement of Peloton density, bacterium bulb diameter and bacterium ball dry weight in fluid nutrient medium, mycelial growth rate in cultivation
Measurement, the measurement of sporophore growth index, the measurement of biological efficiency and total cultivation period etc. measurement, tied by research
Fruit improves Pleurotus eryngii yield it is found that bacillus Bac1 can effectively facilitate mycelial growth rate, and effectively shortens its growth week
Phase, and then increase the economic benefit of Edible Fungi.
Detailed description of the invention
Fig. 1 is the systematic evolution tree according to 16s rRNA sequence construct.
Fig. 2 is influence result figure of the addition Bac1 filtering fermentating liquid in sterilizing front and back to hyphal diameter.
Fig. 3 is that the Bac1 filtering fermentating liquid of sterilizing front and back addition different volumes influences result figure to mycelial growth rate.
Fig. 4 is the influence result figure for adding Bac1 filtering fermentating liquid to dry mycelial weight.
Fig. 5 is the influence result figure for adding Bac1 filtering fermentating liquid to Peloton density.
Fig. 6 is influence of the Bac1 filtering fermentating liquid of the preceding addition different volumes of sterilizing to bacterium ball dry weight.
Fig. 7 is that influence of the Bac1 filtering fermentating liquid of different volumes to bacterium ball dry weight is added after sterilizing.
Fig. 8 is influence of the Bac1 filtering fermentating liquid of sterilizing front and back addition different volumes to bacterium ball dry weight changing rule.
Fig. 9 is influence result figure of the addition Bac1 filtering fermentating liquid to mycelial growth rate before culture material sterilizing.
Influence result figure of the Bac1 filtering fermentating liquid to mycelial growth rate is added when Figure 10 is inoculation.
Specific embodiment
Separation, screening and the identification of 1 strain of embodiment
1, it isolates and purifies
The Pleurotus eryngii bacterium bag that each growth period is randomly selected from Pleurotus eryngii mushroom factory takes Pleurotus eryngii in an aseptic environment
Bacterium bag upper, middle and lower portion each 10g of compost is added in 90mL sterile water, vibrates 30min in 120rpm, 24 DEG C of shaking tables, as
10-1Bacteria suspension, successively according to 10-2, 10-3, 10-4Concentration gradient is diluted, and each gradient dilution liquid 0.2mL is taken to be applied to respectively
On LB plating medium, in 37 DEG C of 24~48h of culture, 3 repetitions of each gradient.Picking has the single bacterium of different shape feature
It falls, after purification through multiple plate streaking, numbers and save.
2, the observation of morphological feature
Bacterial strain colonial morphology is round, glossy and opaque white colony, bacterium colony rough surface, it is flat, in not advising
Then state；It observes under the microscope, cell is in the shape of a rod, and can produce gemma, gemma ellipse；Gram's staining is positive strain.
3, Physiology and biochemistry is identified
The experimental method referring to described in " common bacteria system identification handbook " and " primary Jie Shi systematic bacteriology handbook " is to bacterium
Strain carries out Physiology and biochemistry identification, and qualification result is shown in Table 1.
Table 1Bac1 Bacterial Physiological biochemical character experimental result
The bacterial strain can be grown on the LB culture medium containing 6.5%NaCl, according to qualification result it is found that the bacterial strain and wax-like bud
The characteristic of spore bacillus (Bacillus cereus) matches.
4,16S rRNA sequencing and Phylogenetic Analysis
16S rDNA sequence is carried out to isolated strains to expand, and the purpose band of 1 treaty 1400bp has been obtained, by purpose
The length being sequenced after the recycling of segment glue is 1422bp.The 16S rDNA sequence is subjected to homology sequence comparison, while according to 16S
RDNA sequence construct systematic evolution tree (see Fig. 1), discovery isolated strains and wax printing fabric Bacillus cereus belong to one
A heredity branch, affinity reach 99%.Combining form observation of characteristics and eco-physiological characteristics analysis, determine that isolated strains are
Wax printing fabric.
Below only the influence by Bacillus cercus Bac1 to Growth of Pleurotus eryngii come to a specific embodiment of the invention
It is described in detail.
Influence of the 2 Bacillus cercus Bac1 filtering fermentating liquid of embodiment to Pleurotus eryngii mycelia and bacterium ball
1, culture medium
(1) LB culture medium: yeast powder 0.5%, beef extract 0.5%, peptone 1%, Nacl 1%, (agar 1.5%~
2.0%), 7.2~7.4 pH.
(2) PDA enriched medium: potato 20%, glucose 20%, peptone 0.3%, yeast powder 0.3%, KH2PO4
0.15%, MgSO4·7H2O 0.15%, agar 1.8%~2%, pH are natural.
2, the preparation of Bacillus cercus Bac1 filtering fermentating liquid
Bacillus cercus is inoculated in LB liquid medium, shaking table culture is to thin under the conditions of 37 DEG C, 180r/min
OD after fermented liquid dilutes 2 times600Until value is between 0.6~0.7, so that bacillus Bac1 fermentation liquid is obtained, then through 4
DEG C, 10000r/min centrifugation 20min after, (hereinafter referred to as with sterile organic membrane filtration, as bacillus Bac1 filtering fermentating liquid
Bac1 filtering fermentating liquid), 4 DEG C of refrigerators save backup.
3, Bac1 filtering fermentating liquid is added before and after medium sterilization respectively
In the forward and backward Bac1 filtering fermentating liquid for adding different volumes respectively of PDA enriched medium high pressure sterilization, gradient is every
The filtering fermentating liquid of 1mL, 3mL, 5mL, 7mL, 9mL, 11mL, 20mL are added in 100mL liquid fermentation medium respectively.With addition
LB liquid medium is control group, and ordinary liquid culture medium is CK, 25 repetitions of every group of processing.
3, experimental method
(1) measurement of hyphal diameter
Using inserted sheet cultural method, the coverslip for climbing Pleurotus eryngii mycelia is obtained, finds the clear visual field under the microscope, used
Micro- micrometer measures hyphal diameter, each plane-table operation 20 times.
(2) measurement of mycelial growth rate
Each plate center is quantitatively inoculated with the Pleurotus eryngii bacteria cake of 1 piece of 5mm diameter, cultivates in 25 DEG C of constant incubators, reference
Colony diameter method measures and calculates mycelial growth rate.
(3) measurement of mycelia growth rhythm and Peloton density, diameter and dry weight
Pleurotus eryngii is inoculated with into the PDA enriched medium added with Bac1 filtering fermentating liquid, 7 pieces of diameters of every bottle of inoculation are 5mm
Fungus block, the shaken cultivation under the conditions of 25 DEG C, 120r/min, per being measured by sampling its dry mycelial weight for 24 hours.
In addition, range estimation fluid nutrient medium Peloton density size, then takes 4mL fermentation liquid in training with pipette from conical flask
It supports in ware, counts bacterium ball quantity, repeat statistics 3 times, calculate the averag density of every bottle of strain bacterium ball, while also measuring its diameter
(culture medium on bacterium ball is washed out with distilled water, its moisture is blotted with filter paper, measures its weight) with dry weight.
4, result and analysis
(1) measurement of hyphal diameter
Influence result of the addition Bac1 filtering fermentating liquid in sterilizing front and back to hyphal diameter is shown in Fig. 2.As shown in Figure 2, before sterilizing
Afterwards, with attenuating after the increase hyphal diameter elder generation overstriking of volume, and reach maximum value in 5mL volume, respectively 6.64 μm,
6.91 μm, be 1.21 times, 1.26 times of corresponding control group respectively.Show that mycelia is sturdy when adding 5mL filtering fermentating liquid, gives birth to
Growing way is maximum.
(2) measurement of mycelial growth rate
The Bac1 filtering fermentating liquid of sterilizing front and back addition different volumes influences result to mycelial growth rate and sees Fig. 3.By Fig. 3
It is found that adding Bac1 filtering fermentating liquid before sterilizing, 1~7mL range mycelial growth rate gradually accelerates, and when 7mL reaches maximum value,
For 5.34mm/d, it is 1.07 times of corresponding control group, obviously slows down greater than mL mycelial growth rate, but faster than control group.It goes out
Bac1 filtering fermentating liquid is added after bacterium, 1~5mL range has an apparent facilitation to mycelia growth, and the speed of growth is up to most when 5mL
Big value, is 5.49mm/d, is 1.13 times of corresponding control group, facilitation obviously weakens later.Therefore, before and after medium sterilization
It is more significant to mycelial growth rate facilitation when addition 7mL and 5mL respectively, and the latter is better than the former.
In conclusion sterilizing front and back addition Bac1 filtering fermentating liquid has facilitation to mycelia growth, and the latter is better than
The former.Mycelial growth rate and diameter are maximum when volume is 5mL after sterilizing；Bac1 filtering fermentating liquid, mycelia are added before sterilizing
Diameter and the speed of growth reach maximum value in 5mL and 7mL respectively, therefore add Bac1 filtering fermentating liquid before comprehensively considering sterilizing
Influence to mycelial growth rate and diameter is it is found that mycelium growth vigor is best when additive amount is about 6mL.
(3) mycelia growth rhythm
Dry mycelial weight result of variations is shown in Fig. 4, and Peloton density result of variations is shown in Fig. 5 and table 2.
By Fig. 4 and Fig. 5 it is found that with incubation time extension, dry mycelial weight and Peloton density (range estimation) gradually increase,
Shaking table culture 6d, Peloton density and dry mycelial weight growth rate are most fast.The 1st, 2d is period of delay after shaking table culture, and 4-6d is logarithm
Phase enters lag phase after 6d.It can be seen that Pleurotus eryngii mycelia is in the logarithmic growth later period, at this time mycelia when culture is to 6d
Growing point is most, and vigor is stronger.So determining that the minute of each index of Pleurotus eryngii fermentation liquid mycelia is the after shaking table culture
6d。
(4) influence of the Bac1 filtering fermentating liquid to Peloton density
Shaking flask culture 6d after inoculation, influence of the measurement sterilizing front and back different volumes Bac1 filtering fermentating liquid to Peloton density,
The result is shown in tables 2.When adding FF-5mL after sterilizing as the result is shown, Peloton density is maximum, up to 44.33/mL, with corresponding control group
There are extremely significant difference (P < 1%)；Before sterilizing when addition TF-5mL, Peloton density is up to 39.67/mL, and accordingly compares
Group significant difference (P < 5%).There are extremely significant difference (P < 1%) between FF-5mL and TF-5mL.Peloton density shows more greatly bacterium
Silk growing point is more, and mycelium growth vigor is better, and therefore, FF-5mL and TF-5mL remarkably promote effect to mycelium growth vigor, and preceding
Person's facilitation is more significant.
Influence of the 2 Bac1 filtering fermentating liquid of table to Peloton density
(5) influence of the Bac1 filtering fermentating liquid to bacterium bulb diameter
Influence of the Bac1 filtering fermentating liquid to bacterium bulb diameter the results are shown in Table 3.As shown in Table 3, FF-5mL mistake is added after sterilizing
Fermentation liquid is filtered, the minimum 2.3mm of bacterium bulb diameter, there are significant differences with corresponding control group；TF-5mL is added before sterilizing, bacterium ball is straight
The minimum 2.4mm of diameter, is presented significant difference between corresponding control group.Without significant difference between FF-5mL and TF-5mL.Liquid
In culture medium, Peloton density is big, small in size, and numerous mycelium pellets can increase peripheral growth district volume, makes full use of culture solution
In nutriment, obtain bigger mycelium morphology factor, thus FF-5mL and TF-5mL have to the increase of mycelium morphology factor it is aobvious
Facilitation is write, and the difference between the two is not significant.
Influence of the 3 Bac1 filtering fermentating liquid of table to bacterium bulb diameter
(6) influence of the Bac1 filtering fermentating liquid to bacterium ball dry weight
The Bac1 filtering fermentating liquid of sterilizing front and back addition different volumes is shown in Fig. 6, Fig. 7 and figure to the influence result of bacterium ball dry weight
8.By Fig. 6-8 it is found that with the increase of additive amount before and after sterilizing, the rule of reduction after first increase is presented in dry mycelial weight, is being added
Facilitation is most significant when amount is 5mL, and it is corresponding respectively that in shaking table culture 6d, dry mycelial weight, which is respectively 0.45g and 0.48g,
1.60 times of control group and 1.71 times；5~11mL facilitation obviously weakens, and after being greater than 11mL, dry mycelial weight is lower than CK.Separately
Outside, the addition of Bac1 filtering fermentating liquid does not influence dry mycelial weight changing rule.
3 Bac1 filtering fermentating liquid of embodiment is to the Pleurotus eryngii mycelia of culture material culture and the influence of economical character
1, culture medium
Culture medium for cultivating: sawdust 35%, wheat bran 15%, corncob 32.5%, corn flour 5%, rice bran 10%, calcium carbonate
1.5%, lime 1%.
2, experimental method
(1) Bac1 filtering fermentating liquid is added before medium sterilization
The Bac1 filtering fermentating liquid of different volumes gradient is mixed into sterile water, planting almond abalone mushroom culture medium is prepared.Finally
Content is that the Bac1 in every 120g wet feed (i.e. every bottle of culture material) containing 5mL, 10mL, 15mL, 20mL, 40mL, 60mL filters hair
Zymotic fluid.To add LB liquid medium as control group, sterile water CK, 25 repetitions of every group of processing.
(2) Bac1 filtering fermentating liquid is added when being inoculated with Pleurotus eryngii
When being inoculated with pleurotus eryngii quel strains add different volumes Bac1 filtering fermentating liquid, gradient are as follows: 2mL, 4mL, 6mL,
8mL, 10mL, 12mL, 20mL, 40mL, to add LB liquid medium as control group, common cultivation material is CK, every group of processing 25
A repetition.Enter oese section after completing addition work, every bottle of culture material is inoculated with the fungus block that 2 pieces of diameters are 1cm.
(3) long to 1/2 full bottle of mycelia when add Bac1 filtering fermentating liquid
Common cultivation bottle after taking inoculation, the Bac1 filtering of addition different volumes gradient when mycelia grows to 1/2 full bottle
Fermentation liquid, gradient are as follows: 2mL, 4mL, 6mL, 8mL, 10mL, 12mL, 20mL, 40mL, to add LB liquid medium as control group,
Common cultivation material is CK, 25 repetitions of every group of processing.
(4) Bac1 filtering fermentating liquid is added when mycelia length to full bottle
Common cultivation bottle after taking inoculation grows to full bottle addition different volumes Bac1 filtering fermentating liquid, gradient to mycelia
Are as follows: 2mL, 4mL, 6mL, 8mL, 10mL, 12mL, 20mL, 40mL, to add LB liquid medium as control group, common cultivation material
For CK, 25 repetitions of every group of processing.
Sporophore shape obtained by above-mentioned every kind of processing and quality index and biological efficiency are measured:
1. the measurement of sporophore shape and quality index
Bacteria cover diameter, stem length, stem diameter are measured with graduated scale, single mushroom fresh weight and the common day of mycelia material feeding amount
Flat measurement, single mushroom volume are measured with drainage.
The bulk density (g/mL) of fructification=mono- mushroom weight (g)/mono- mushroom volume (mL)
2. the measurement of biological efficiency
It after harvesting fructification, is weighed immediately with general utility balance, records single mushroom output of fresh of fructification respectively, calculate biology
Learn conversion ratio.
Biological efficiency (%)=fresh mushroom weight (g)/siccative weight (g) × 100%
3, result and analysis
(1) influence of the Bac1 filtering fermentating liquid to mycelial growth rate and fructification is added before medium sterilization
1. the influence to mycelial growth rate
When making culture material, participation high temperature after deionized water spice is replaced to go out with the Bac1 filtering fermentating liquid of different volumes
Bacterium.Using sterile LB liquid medium as control group, culture material without any processing is CK, measures mycelia under the conditions of different disposal
As a result the speed of growth is shown in Fig. 9.
As shown in Figure 9, with the increase of Bac1 filtering fermentating liquid volume, the presentation of mycelia growth promoting function is first increased and is subtracted afterwards
Small trend.Mycelial growth rate is most fast when 10mL additive amount, is 5.83mm/d, improves 13.18% compared with the control group；Greatly
Facilitation is gradually reduced after 10mL.Show after bacillus Bac1 filtering fermentating liquid sterilizes with culture material to Pleurotus eryngii mycelia
The speed of growth remarkably promotes effect.
2. the influence to fruiting body yield and biological efficiency
Shadow of the Bac1 filtering fermentating liquid of addition different volumes to Pleurotus eryngii fruiting body yield and biological efficiency before sterilizing
Sound the results are shown in Table 4.As shown in Table 4, different volumes Bac1 filtering fermentating liquid processing after fructification list mushroom weight be 29.74g~
39.51g, biological efficiency are 59.49%~78.86%, and single mushroom yield highest after FF-10mL is handled, is 39.51g
(78.86%), followed by FF-15mL, yield are 36.06g (70.12%), are 1.29 times and 1.14 of corresponding control group respectively
Times, there are significant differences with other processing groups.Therefore, FF-10mL is remarkably improved Pleurotus eryngii yield.
Influence of the 4 Bac1 fermentation liquid of table to Pleurotus eryngii yield
3. the influence to sporophore shape
Influence of the Bac1 filtering fermentating liquid of addition different volumes to Pleurotus eryngii sporophore shape the results are shown in Table 5 before sterilizing.
As shown in Table 5, after FF-10mL is handled, mushroom body is larger, and single mushroom volume and bulk density are maximum, bacteria cover diameter, stem length, stem
Diameter, single mushroom volume and bulk density are respectively 4.79cm, 5.58cm, 2.7cm, 52.68mL and 0.74g/mL, and wherein bulk density data are aobvious
Show, with other processing there are significant difference (P < 1%), shows that the fructification tissue consolidation under the treatment conditions is fine and close.Followed by
FF-15mL, bacteria cover diameter, stem length, stem diameter, single mushroom volume and bulk density be respectively 5.32cm, 5.74cm, 2.58cm,
51.51mL and 0.68g/mL.In conclusion Pleurotus eryngii individual is larger in addition FF-10mL processing before sterilizing, single mushroom weight, single mushroom
Volume and bulk density are maximum, are conducive to improve mushroom body consistency, for optimal processing when preceding addition filtering fermentating liquid that sterilizes.
Influence of the 5 Bac1 fermentation liquid of table to Pleurotus eryngii form
(2) influence of the Bac1 filtering fermentating liquid to mycelial growth rate and fructification is added when being inoculated with
1. the influence to mycelial growth rate
After culture material sterilizing is cooled to room temperature, the bacillus Bac1 fermentation liquid for adding different volumes is raw to Pleurotus eryngii mycelia
The influence of long speed, the result is shown in Figure 10.
As shown in Figure 10, bacillus Bac1 fermentation liquid and Bac1 filtering fermentating liquid have facilitation to mycelia growth,
And the former facilitation is more significant.With the increase of bacillus Bac1 fermentation liquid addition volume, mycelial growth rate is gradually accelerated,
In 6mL, the speed of growth is most fast, is 6.07mm/d, improves 18.77% compared with the control, the section the 8~10mL speed of growth by
Decrescence slow, mycelia growth is suppressed after being greater than 10mL.The section 2~8mL mycelial growth rate in Bac1 filtering fermentating liquid processing group
Gradually accelerate, the speed of growth reaches maximum value when 8mL, is 5.93mm/d, improves 13.86% compared with the control, is greater than raw after 8mL
Long speed slows down.In conclusion inoculation when add 6mL bacillus Bac1 fermentation liquid to cultivar mycelia growth-promoting effect most
Be it is obvious, sterilize before addition 10mL filtering fermentating liquid take second place.
2. the influence to fruiting body yield and biological efficiency
The bacillus Bac1 fermentation liquid and filtering fermentating liquid for adding different volumes when inoculation are to Pleurotus eryngii fruiting body yield
Influence with biological efficiency the results are shown in Table 6.As shown in Table 6, the bacillus Bac1 fermentation liquid of different volumes and filtering fermentation
Fructification list mushroom weight is 22.28g~33.42g after liquid processing, and biological efficiency is 44.32%~66.84%, fermentation liquor treatment
BF-6mL is added in group, single mushroom yield highest is 33.42g (66.85%) to be 1.10 times accordingly compareed and exist therewith aobvious
It writes difference (P < 5%)；When adding FF-8mL in filtering fermentating liquid processing group, it is corresponding that single mushroom yield, which is 32.17g (64.25%),
1.05 times of control.Difference is not significant between BF-6mL and FF-8mL.Therefore, BF-6mL and FF-8mL is added when inoculation facilitates
Improve Pleurotus eryngii yield.
Influence of the 6 Bac1 fermentation liquid of table to Pleurotus eryngii yield
3. the influence to sporophore shape
The bacillus Bac1 fermentation liquid and filtering fermentating liquid for adding different volumes when inoculation are to Pleurotus eryngii sporophore shape
Influence the results are shown in Table 7.As shown in Table 7, BF-6mL is added in fermentation liquor treatment group, bacteria cover diameter, stem length, stem are straight
Diameter, single mushroom volume and bulk density are respectively 4.46cm, 5.34cm, 2.34cm, 50.19mL and 0.65g/mL, in terms of bulk density with phase
Answering control group, there are extremely significant difference (P < 1%).Followed by BF-4mL, there are significant differences in terms of bulk density with BF-6mL.It crosses
Filtering additive amount in fermentation liquor treatment group is the section 6~8mL (FF-6mL~FF-8mL), and bulk density is 0.64g/mL, and the latter's body
Product is 50.15mL, and difference reaches the level of signifiance in terms of bulk density with BF-6mL.It can be seen from the above, BF-6mL is added when inoculation,
Mushroom body is larger, and single mushroom yield and volume are maximum, meat bacteria organization's consistency highest, for the optimal processing for adding sample liquid when inoculation.
Influence of the 7 Bac1 fermentation liquid of table to Pleurotus eryngii form
Influence of the Bac1 fermentation liquid to fructification is added at (3) 1/2 full bottles
1. the influence to fruiting body yield and biological efficiency
When long to 1/2 full bottle of mycelia, the bacillus Bac1 fermentation liquid and filtering fermentating liquid for adding different volumes are to apricot
The influence of abalone mushroom fruiting body yield and biological efficiency the results are shown in Table 8.As shown in Table 8, the bacillus Bac1 hair of different volumes
Zymotic fluid and filtering fermentating liquid processing after fructification list mushroom weight be 24.74g~40.58g, biological efficiency be 49.50%~
81.21%, in fermentation liquor treatment group, single mushroom weight optimal processing is BF-8mL, is 40.58g (81.21%), is corresponding control group
1.32 times and there is extremely significant difference, followed by BF-6mL therewith, be 39.13g.Difference is not shown between BF-8mL and BF-6mL
It writes, has notable difference with remaining processing group.Filtering fermentating liquid optimal processing is FF-10mL, is 39.01g (76.50%), is phase
It answers 1.26 times of control group and there is extremely significant difference therewith.BF-8mL and FF-10mL difference is not significant.Therefore, BF- is added
8mL and FF-10mL is remarkably improved Pleurotus eryngii yield.
Influence of the 8 Bac1 fermentation liquid of table to Pleurotus eryngii yield
2. the influence to sporophore shape
When long to 1/2 full bottle of mycelia, the bacillus Bac1 fermentation liquid and filtering fermentating liquid for adding different volumes are to apricot
The influence of abalone mushroom sporophore shape the results are shown in Table 9.As shown in Table 9, BF-8mL, bacteria cover diameter, bacterium are added in fermentation liquor treatment group
Handle length, stem diameter, single mushroom volume and bulk density are respectively 4.98cm, 6.04cm, 2.90cm, 54.23mL and 0.75g/mL,
There are extremely significant poor (P < 1%) with corresponding control group for middle bulk density.Followed by BF-6mL, difference is not shown in terms of bulk density with BF-8mL
It writes, and the latter's volume is larger.Optimal additive amount is FF-10mL, bacteria cover diameter, stem length, bacterium in filtering fermentating liquid processing group
Shank diameter, single mushroom volume and bulk density are respectively 5.16cm, 5.72cm, 2.64cm, 52.89mL and 0.74g/mL, and are come from bulk density
It sees, it is significant with BF-8mL otherness.Therefore, the mushroom body in addition BF-8mL processing is maximum, and single mushroom volume and bulk density are also most
Big, illustrate that the processing group mushroom body tissue degree of packing is higher.In conclusion the mycelia adds sample liquid when growing to 1/2 full bottle
Optimal processing be BF-8mL.
Influence of 9 fermentation liquid of table to Pleurotus eryngii form
(4) influence of the Bac1 fermentation liquid to fructification is added when full bottle
1. the influence to fruiting body yield and biological efficiency
When mycelia length to full bottle, the bacillus Bac1 fermentation liquid and filtering fermentating liquid for adding different volumes are to Pleurotus eryngii
The influence of fruiting body yield and biological efficiency the results are shown in Table 10.As shown in Table 10, the bacillus Bac1 fermentation of different volumes
Liquid and filtering fermentating liquid processing after fructification list mushroom weight be 21.48g~35.61g, biological efficiency be 43.00%~
71.49%, in fermentation liquor treatment group, single mushroom weight optimal processing is BF-8mL, be 35.61g (71.49%) is corresponding control group
1.17 times and there is significant difference, followed by BF-6mL therewith, is 33.19g.Difference is not significant between BF-8mL and BF-6mL,
The two and remaining processing group have notable difference.Filtering fermentating liquid optimal processing is FF-10mL, is 33.62g (67.25%), is phase
1.11 times for answering control group.BF-8mL and FF-10mL difference is not significant.Therefore, BF-8mL and FF-10mL is added when mycelia expires bottle
There is facilitation to Pleurotus eryngii yield.
Influence of the 10 Bac1 fermentation liquid of table to Pleurotus eryngii yield
2. the influence to sporophore shape
When mycelia length to full bottle, the bacillus Bac1 fermentation liquid and filtering fermentating liquid for adding different volumes are to Pleurotus eryngii
The influence of sporophore shape the results are shown in Table 11.As shown in Table 11, BF-8mL, bacteria cover diameter, stem are added in fermentation liquor treatment group
Length, stem diameter, single mushroom volume and bulk density are respectively 4.58cm, 5.70cm, 2.42cm, 51.06mL and 0.70g/mL, wherein
There are extremely significant difference (P < 1%) with corresponding control group for bulk density.Followed by BF-10mL, there are poles in terms of bulk density with BF-8mL
Significant difference.Optimal additive amount is FF-10mL, bacteria cover diameter, stem length, stem diameter, list in filtering fermentating liquid processing group
Mushroom volume and bulk density are respectively 4.40cm, 5.62cm, 2.38cm, 49.90mL and 0.67g/mL.In terms of bulk density BF-8mL with
There are extremely significant differences by FF-10mL.In short, Pleurotus eryngii mushroom body is larger under addition BF-8mL treatment conditions, single mushroom weight, single mushroom body
Long-pending and bulk density is maximum, is the optimal processing that sample liquid is added when mycelia expires bottle.
Influence of the 11 Bac1 fermentation liquid of table to the sub- form of Pleurotus eryngii
(5) influence of the Different treatments to growth cycle
Different time adds Bac1 fermentation liquid, and influence to Growth of Pleurotus eryngii period when additive amount is best, as a result sees
Table 12.As shown in Table 12, at long to 1/2 full bottle of mycelia, the cultivation period of addition 8mL fermentation liquor treatment group is most short, is 43d, than
Control group shortens 5d, with other addition periods there are significant difference, be mainly manifested in promote mycelia growth, former base formed and
In terms of sporophore growth；10mL filtering fermentating liquid is followed by added before sterilizing, 4d is shortened than control group, to mycelia and fructification
Growth is obviously promoted effect；8mL fermentation liquid is added when adding 6mL fermentation liquid and full bottle when inoculation, is contracted compared with the control group
Short 2d, the former, which is mainly manifested in, promotes mycelia growth aspect, and the latter, which is mainly manifested in, promotes former base to be formed and sporophore growth
Aspect.Therefore, long to 1/2 full bottle of mycelia when addition 8mL fermentation liquid can significantly shorten the Growth of Pleurotus eryngii period.
12 different times of table add influence of the Bac1 fermentation liquid to the Growth of Pleurotus eryngii period
(6) combined influence of the Different treatments to Growth of Pleurotus eryngii
Different time add Bac1 fermentation liquid, and additive amount be it is best when 13 are shown in Table to the synthesis result of Growth of Pleurotus eryngii.
As shown in Table 13, different times are developed in Growth of Pleurotus eryngii, adds the optimal processing group filtered out and carries out experiment in cultivation, mycelia
Addition sample liquid list mushroom weight is most heavy when growing to 1/2 full bottle, is 40.58g, and fructification value of bulk density is maximum, is 0.75g/mL, biology
Efficiency highest is learned, is 81.22%, it is 43d that cultivation period is most short, hence it is evident that shortens the Growth of Pleurotus eryngii period while improving sub real
Body yield, each index comprehensive sequence are optimal.Addition processing before followed by sterilizing, addition is handled when being full bottle again, is finally connect
Addition is handled when kind.Therefore mycelia when growing to 1/2 full bottle addition sample liquid be more advantageous to and improve Pleurotus eryngii yield and shorten its life
Long period.
Combined influence of 12 Different treatments of table to Growth of Pleurotus eryngii
In conclusion in Growth of Pleurotus eryngii development different times addition different disposal, the bacillus Bac1 of different volumes
Fermentation liquid grows mycelia, former base is formed and sporophore growth has different degrees of facilitation.In terms of mycelia growth,
Sterilizing before addition and inoculation when addition Bac1 fermentation liquid remarkably promote effect, respectively addition 10mL filtering fermentating liquid with
Reach maximum value 5.83mm/d and 6.07mm/d when 6mL fermentation liquid, amplification is respectively 13.18% He compared with corresponding control
18.77%.In terms of sporophore growth, addition 8mL fermentation liquid growth-promoting effect is most significant when mycelia grows to 1/2 full bottle,
Middle list mushroom volume is 54.23mL, and single mushroom weighs 40.58g, and fructification bulk density is 0.75g/mL, biological efficiency 81.22%.
Cultivation period shortens 5d.The Pleurotus eryngii mushroom body produced in the processing is larger, single mushroom yield highest, meat bacteria organization's consolidation, raw
Object transformation efficiency highest and be obviously shortened cultivation the production cycle, wherein biological conversion rate improve 21.22%.Followed by sterilize
Preceding addition 10mL filtering fermentating liquid, adds 8mL fermentation liquid when being full bottle again, 6mL fermentation liquid is added when being finally inoculation.Always
It, bacillus Bac1 fermentation liquid can promote cultivar mycelial growth rate, improve Pleurotus eryngii yield, and effectively shorten its growth
Period.
Claims (8)
1. a kind of Bacillus cercus, is named as Bac1, Chinese microorganism strain preservation management was preserved on September 17th, 2018
Committee's common micro-organisms center, deposit number are CGMCC No.16475.
2. Bacillus cercus as described in claim 1 is promoting the application in edible fungi growth.
3. Bacillus cercus according to claim 1 is promoting the application in edible fungi growth, which is characterized in that edible
Bacterium is Pleurotus eryngii.
4. Bacillus cercus according to claim 2 or 3 is promoting the application in edible fungi growth, which is characterized in that
Bacillus cercus Bac1 be added the time be culture material sterilizing before or vegetative stage.
5. Bacillus cercus according to claim 4 is promoting the application in edible fungi growth, which is characterized in that wax-like
Bacillus Bac1 addition when mycelia grows at bacterium bottle or bacterium bag 1/2.
6. Bacillus cercus according to claim 4 is promoting the application in edible fungi growth, which is characterized in that wax-like
The adding manner of bacillus Bac1 is added in a manner of fermentation liquid.
7. Bacillus cercus according to claim 6 is promoting the application in edible fungi growth, which is characterized in that wax-like
The additional amount of bacillus Bac1 fermentation liquid is the 5%~10% of culture material weight.
8. a kind of edible mushroom promotor, which is characterized in that including Bacillus cercus Bac1 described in claim 1.
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| CN114830969A (en) * | 2022-06-07 | 2022-08-02 | 云南菌视界生物科技有限公司 | Organic compound capable of shortening cultivation period of tremella aurantialba and application thereof |
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