CN103340156B - Novel strain of lyophyllum decastes - Google Patents
Novel strain of lyophyllum decastes Download PDFInfo
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- CN103340156B CN103340156B CN201310098139.XA CN201310098139A CN103340156B CN 103340156 B CN103340156 B CN 103340156B CN 201310098139 A CN201310098139 A CN 201310098139A CN 103340156 B CN103340156 B CN 103340156B
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Abstract
The invention relates to a novel strain of lyophyllum decastes: Finc-LD-4, with a preservation number being CCTCC NO: M 2013008. The Finc-LD-4 strain has characteristics of good stipe length and uniformity in per plant, flat and lotus-shaped pileus, good anti-competitor capability and concentrated harvesting stage, and is suitable for industrialized popularization and planting.
Description
[technical field]
The present invention relates to edible mushroom technical field, or rather, relate to a kind of Lyophyllum decastes bacterial strain.
[background technology]
Lyophyllum decastes (Latin title Lyophyllum decastes), be subordinate to Basidiomycotina, Hymenomycetes, Agaricales, Bai Mo section, from pleat umbrella, belong to, to eat the good wild edible fungus that Mycota is competitively studied cultivation both at home and abroad, Lyophyllum decastes bacterial context is plump fine and smooth, A sweety scent assails the nostrils, the crisp cunning of mouthfeel, delicious flavour, nutritious, and to the repair of human body cell with having demonstrated great superiority aspect treatment cardiovascular disease, anti-ageing waiting for a long time, be a kind of famous and precious edible mushroom that eats medicine dual-purpose.Since 2006, Lyophyllum decastes started a small amount of artificial cultivation and succeeds in Japan.
[summary of the invention]
The features such as the technical problem that the present invention solves is to provide a kind of Lyophyllum decastes bacterial strain Finc-LD-4, and this bacterial strain has stem length high conformity in individual plant, and anti-miscellaneous bacteria ability is strong, and picking time is concentrated, suitable batch production popularizing planting.
The present invention is achieved by the following technical solutions, and Lyophyllum decastes bacterial strain Finc-LD-4 of the present invention is by gathering the wild resource in Yunnan, through domesticating and cultivating, then obtaining through screening system.This bacterial strain has been preserved in Chinese Typical Representative culture collection center, address on January 18th, 2013: Wuhan, China city Wuhan University, its preserving number is CCTCC NO:M2013008.
Lyophyllum decastes bacterial strain Finc-LD-4 of the present invention is characterised in that:
1, morphological feature
The mycelia of Finc-LD-3: aerial hyphae is dense; Cap: diameter is 3 ± 2cm, and it is light yellow that color is, circle, there is tiny fine hair on cap surface, and cap is flat, and very easily parachute-opening is patellate, and quality is tender and crisp, frangible; Lamella: light yellow, lamella section is crescent; Stem: length is 6～15cm, and stem top is pale yellow, and bottom is pure white, near the stem interior solid consolidation at cap place, away from cap place stem hollow, stem has scale, scale main raw in the middle and upper part near cap, distribute irregular, stem clusters, and refers to Fig. 1, Fig. 2.
2, biological property
Nutrient component source: wood chip, corncob, rice bran, wheat bran, corn flour, cotton seed hulls, soybean skin etc., wherein, water content 64%～66% (percentage by weight, lower same).
Cultivate and cultivation condition: Mycelium culture temperature is 20～25 ℃, humidity 75%, CO
2concentration 2000～3500ppm, cultivation cycle 50～70 days.Suitable fruiting temperature is 15～18 ℃, humidity 93%～100%, CO
2concentration 1000～2000ppm, first 1～7 day, intensity of illumination 10～50Lux, latter 8～32 days intensity of illumination 200～300Lux, every day, clearance-type was opened layer bracket lamp stimulation fruiting, and the cultivation cycle is about 32 days.
3, genetics characteristic
The genomic DNA of Lyophyllum decastes Finc-LD-4 bacterial strain carries out pcr amplification through universal primer ITS1 and ITS4, obtains the specific DNA segment that size is about 691bp, and this fragment is checked order, and sequence information is referring to sequence table.
The new bacterial strain of Finc-LD-4 of the present invention, there is stem length high conformity in individual plant, fruit body exterior quality is good, the features such as anti-miscellaneous bacteria ability is strong, has reduced production management difficulty, and picking time is concentrated, reduced widely the manual work intensity of production link, and long fresh-keeping period, per unit area yield is high, suitable batch production popularizing planting.
[accompanying drawing explanation]
Fig. 1 is the fruit body outward appearance picture of Lyophyllum decastes Finc-LD-4 bacterial strain of the present invention.
Fig. 2 is Lyophyllum decastes Finc-LD-4 bacterial strain phylogenetic tree of the present invention.
[embodiment]
Below by specific embodiment, the present invention is described in further detail.
The domestication of embodiment 1 Finc-LD-4 of the present invention
The present embodiment has illustrated the domestication process of Lyophyllum decastes Finc-LD-4, and concrete steps are as follows:
1) preparation medium
PDA medium: every 1L medium potato 200g, glucose 20g, agar 20g.
2) collection of resources
In September, 2007, in 1800～3000 meters of height above sea level areas of Yunnan Binchuan Jizu Shan Mountain, gathers wild resource 4 strains, takes back laboratory, organize separation, be inoculated on PDA medium, obtain and treat 4 of naturalized strains, numbering is respectively FLD-A, FLD-B, FLD-C, FLD-D.
3) strain domestication
Use PDA medium to expand the FLD-D in 4 bacterial strains that obtain numerous, 22 ℃, humidity 70%, gas concentration lwevel is less than 3000ppm, dark culturing.
To the cultivation of going down to posterity respectively of above-mentioned 4 bacterial strains, numerous 20 of each expansion, inoculated after 12～15 days, chose two test tube subcultures that a bacterium is the fastest and expanded numerous, be seeded on PDA medium, inoculate after 12～15 days, then get and send out two test tube subcultures that bacterium is the fastest and expand numerously, repeat 10 circulations, to expanding numerous offspring's test tube, to send out bacterium speed very fast, send out the neat homogeneous of bacterium speed, complete preliminary domestication work, and with liquid nitrogen cryopreservation, bacterial strain is preserved in to academy of agricultural sciences, Shanghai City edible mushroom research institute.
4) factory formula screening
Through the screening of many wheel formula tests, obtain female kind and cultivated species formula, wherein,
Mother culture media: wood chip 50% (percentage by weight, lower same), rice bran 25%, wheat bran 15% and corn flour 10%, water content is 60%～62%.
Cultivated species medium: wood chip 15%, corncob 25%, cotton seed hulls 25%, rice bran 10%, wheat bran 20% and corn flour 5%, water content is 64%～66%.
5) obtain aimed strain: by a series of domestication breeding, work, obtain a Lyophyllum decastes bacterial strain, it has stem length high conformity in individual plant, anti-miscellaneous bacteria ability is strong, cultivation link does not need earthing, the feature such as concentrates picking time, has reached the object of domestication breeding strain excellent.This bacterial strain is named as " Finc-LD-4 ".
Lyophyllum decastes bacterial strain Finc-LD-4 of the present invention is in being preserved in Chinese Typical Representative culture collection center on January 18th, 2013, and its preserving number is CCTCC NO:M2013008, and bacterial strain is survived after testing, and classification position is for to belong to Lyophyllum decastes from pleat umbrella.
The impact of embodiment 2 carbon sources on Lyophyllum decastes mycelial growth
For trying carbon source: glucose, sucrose and soluble starch.
The basal medium of test carbon source: magnesium sulfate 0.5g, potassium dihydrogen phosphate 1g, peptone 2g, agar 20g, add water to 1000ml.
In basal medium, add respectively for trying a kind of of carbon source, addition is 20g, after mixing, pack in 250ml triangular flask, every bottle of 150ml, puts into 121 ℃ of sterilizing 30min of autoclave, after slightly cooling, is down flat plate, the about 20ml medium of every plate, each dull and stereotyped central authorities connects the bacterium piece that a diameter is 5mm, and each processing arranges 5 repetitions, is placed under 25 ℃ of constant temperatures continuous culture 20 days.Within every 7 days, by right-angled intersection method, measure colony diameter, by the difference of twice test number, come divided by interval number of days, be the mycelial growth rate of average every day.
The impact of table 1 carbon source on Finc-LD-4 and FLD-D mycelial growth
As shown in Table 1, from 3 kinds, for examination carbon sources, FLD-D is to take in the medium that sucrose is carbon source mycelial growth the fastest, and average growth rate reaches 0.68cm/d (centimetre/day, lower with), is secondly starch, is finally glucose; Finc-LD-4 of the present invention, to take in the medium that sucrose is carbon source mycelia average growth rate the fastest, has reached 0.68cm/d, than the fast 0.09cm/d of wild strain, in starch and glucose growth partially slow, average growth rate is 0.68cm/d and 0.63cm/d.Explanation thus, in the medium of different carbon sources, the average mycelial growth rate of Finc-LD-4 is all fast than wild strain, and sucrose is best, and the uniformity of growth rate is better, and excursion is little.
The impact of embodiment 3 nitrogenous sources on Lyophyllum decastes mycelial growth
For trying nitrogenous source: ammonium sulfate, peptone and wheat bran.
The basal medium of test nitrogenous source: magnesium sulfate 0.5g, potassium dihydrogen phosphate 1g, glucose 20g, agar 20g, add water to 1000ml.
In the basal medium of mensuration nitrogenous source utilization, add respectively for trying a kind of of nitrogenous source, addition is 2g (absolute mass of N), after mixing, pack in 250ml triangular flask, every bottled 150ml, puts into 121 ℃ of sterilizing 30min of autoclave, after slightly cooling, is down flat plate, the about 20ml medium of every plate, each dull and stereotyped central authorities connects the bacterium piece that a diameter is 5mm, and each processing arranges 5 repetitions, is placed under 25 ℃ of constant temperature culture conditions continuous culture 20 days.Within every 7 days, by right-angled intersection method, measure colony diameter, by the difference of twice test number, come divided by interval number of days, be the mycelial growth rate of average every day.
The impact of table 2 nitrogenous source on Finc-LD-4 and FLD-D mycelial growth rate
As shown in Table 2, from 3 kinds, for examination nitrogenous sources, FLD-D is to take in the medium that wheat bran is nitrogenous source mycelial growth the fastest, and average growth rate is 1.74cm/d, is secondly peptone, is finally ammonium sulfate, only 0.71cm/d; And Finc-LD-4 of the present invention has reached 1.81cm/d take mycelia average growth rate in the medium that wheat bran is nitrogenous source, than the fast 0.09cm/d of wild strain, in peptone, growth rate is taken second place, and is 1.01cm/d, it is the slowest that ammonium sulfate is that nitrogenous source is grown, and is 0.80cm/d.Show thus, in the medium of different nitrogen sources, the average mycelial growth rate of Finc-LD-4 is all fast than wild strain, and wheat bran is best, and peptone takes second place, and ammonium sulfate is the poorest.
The impact of embodiment 4 C/N ratios on Lyophyllum decastes mycelial growth
Take glucose as carbon source, ammonium sulfate is nitrogenous source, use magnesium sulfate 0.5g, yeast extract 2g, potassium dihydrogen phosphate 1g, agar 20g, add water to 1000ml, preparation C/N ratio (C/N) is respectively 5 kinds of medium of 20:1,30:1,40:1,50:1 and 60:1, packs in 250ml triangular flask, every bottled 120ml, pH is adjusted into 6.0, puts into 121 ℃ of sterilizing 30min of autoclave, after slightly cooling, is down flat plate.The about 20ml medium of every plate, the bacterium piece that is 5mm at each dull and stereotyped central authorities inoculation one diameter, the processing of each C/N ratio arranges 5 repetitions, is placed under 25 ℃ of constant temperatures continuous culture 20 days.Within every 5 days, by right-angled intersection method, measure colony diameter, twice test number is poor divided by interval number of days, draws the mycelial growth rate of average every day, the results are shown in Table 3.
The impact of table 3 C/N ratio on the mycelial growth of Finc-LD-4 and FLD-D
As known from Table 3, when C/N ratio increases to 60:1 by 20:1, the mycelial growth rate variation tendency of Finc-LD-4 and two bacterial strains of FLD-D is not obvious, when C/N ratio is 50:1, all reach maximum growth rate, Finc-LD-4 is 0.51cm/d, and uniformity is better, and FLD-D is 0.42cm/d.Show thus, Finc-LD-4 bacterial strain of the present invention is cultivated in the medium of different C/N ratios, stronger compared with the adaptive capacity of wild strain FLD-D, send out bacterium speed faster, meanwhile, Finc-LD-4 bacterial strain is suitable grows in the medium of various C/N ratios, stronger to the adaptive capacity of medium.
The impact of embodiment 5 pH on Lyophyllum decastes mycelial growth
Sodium hydroxide solution with 1.0% and 1.0% hydrochloric acid solution regulate the acid-base value of PDA medium, obtain pH value and are respectively 6 soda acid gradients of 4.0,5.0,6.0,7.0,8.0 and 9.0.The medium of gained is respectively charged in 250ml triangular flask, and every bottle of 120ml, puts into 121 ℃ of sterilizing 30min of autoclave, the slightly cooling plate that is down flat.The dull and stereotyped about 20ml medium of every culture dish, after completely cooling solid plate.During inoculation, one of the bacterium colony of the aseptic card punch cut-off footpath 5mm of use sterilizing, is seeded in the dull and stereotyped central authorities of PDA.Same pH condition is done 5 repetitions, is placed under 25 ℃ of constant temperature culture conditions continuous culture 20 days.Within every 5 days, by right-angled intersection method, measure colony diameter, the difference of measuring numerical value twice, again divided by interval number of days, draws the mycelial growth rate of average every day, the results are shown in Table 4.
The impact of table 4pH on Finc-LD-4 and FLD-4 mycelial growth
As shown in Table 4, the optimal pH of Finc-LD-4 and the required medium of FLD-D is all 8.0, and mycelial growth rate is respectively 0.76cm/d and 0.97cm/d, and Finc-LD-4 is slightly faster than FLD-D growth rate.And, in the medium of identical pH condition, to cultivate, the mycelial growth rate of Finc-LD-4 is all slightly fast than FLD-D.
Embodiment 6 morphological features and biological property
Morphological feature: aerial hyphae is dense; Cap: diameter is 3 ± 2cm, and it is light yellow that color is, circle, there is tiny fine hair on cap surface, and cap is flat, and very easily parachute-opening is patellate, and quality is tender and crisp, frangible; Lamella: light yellow, lamella section is crescent; Stem: length is 6～15cm, and stem top is pale yellow, and bottom is pure white, near the stem interior solid consolidation at cap place, away from cap place stem hollow, stem has scale, scale main rawly in the middle and upper part near cap, distribute irregular, stem clusters.
Biological property: nutrient component source: wood chip, corncob, rice bran, wheat bran, corn flour, cotton seed hulls, soybean skin etc., wherein, water content 64%～66% (percentage by weight, lower same).
Cultivate and cultivation condition: Mycelium culture temperature is 20～25 ℃, humidity 75%, CO
2concentration 2000～3500ppm, cultivation cycle 50～70 days.Suitable fruiting temperature is 15～18 ℃, humidity 93%～100%, CO
2concentration 1000～2000ppm, first 1～7 day, intensity of illumination 10～50Lux, latter 8～32 days intensity of illumination 200～300Lux, every day, clearance-type was opened layer bracket lamp stimulation fruiting, and the cultivation cycle is about 32 days.
Embodiment 7 cultivation comparative trials
The present embodiment passes through from the new bacterial strain Finc-LD-4 of pleat umbrella, with without habituation of wild bacterial strain FLD-D, cultivate contrast, investigate the anti-miscellaneous bacteria ability of strains tested, the index such as a situation arises, the concentration degree of gathering and per unit area yield, the individual plant fruit body stem length uniformity of the former base (forming fruit body in the future) under earthing condition not, to embody this bacterial strain, be applicable to industrial bottle and plant advantage and the feature of producing.
The cultivated species medium that the present embodiment adopts is: wood chip 15%, and corncob 25%, cotton seed hulls 25%, rice bran 10%, wheat bran 20% and corn flour 5%, water content is 64%～66%.
Cultivation stage pollution rate test take first preculture again the mode of common cultivation carry out, inoculate 128 bottles, be then placed in the pre-incubation chamber that cleanliness factor is high and cultivate 15 days, go to again common culturing room, total cultivation cycle 64 days, adds up pollution condition during mycelium stimulation, result is consulted table 5.
As known from Table 5, the Lyophyllum decastes bacterial strain Finc-LD-4 pollution rate obtaining by domestication is 0.78%, and wild strain pollution rate is 3.91%.As can be seen here, compare with wild strain, Finc-LD-4 has stronger anti-miscellaneous bacteria infection ability.
Table 5Finc-LD-4 and FLD-D cultivation stage pollute contrast
In cultivation process, to take above-mentioned 128 inoculation bottles be research object to former base generation number, and the in the situation that of earthing not, a situation arises in Table 6 for the former base of Finc-LD-4 and wild strain FLD-D.As known from Table 6, the Lyophyllum decastes bacterial strain Finc-LD-4 obtaining by domestication, compares with wild strain, and former base proportion is higher, stronger for the adaptive capacity of soil covering culture not.
A situation arises contrasts for table 6Finc-LD-4 and the former base of FLD-D
Same batch of concentration degree of gathering of statistics while gathering, above-mentioned 128 inoculation bottles of take are subjects, result of the test is consulted table 7.As known from Table 7, the Lyophyllum decastes bacterial strain Finc-LD-4 obtaining by domestication concentrates on first and gathers, and recovery ratio reaches 70.25%, and gathering of FLD-D be take second batch as main, and recovery ratio is 60.00%.As can be seen here, compare with wild strain, the fruit body of Finc-LD-4 cycle of gathering is more concentrated, and concentrates the date of gathering obviously to shift to an earlier date compared with wild strain.
Table 7Finc-LD-4 and the FLD-D concentration degree of gathering contrasts
The subjects of measuring for per unit area yield is above-mentioned 128 inoculation bottles, as known from Table 8, the Lyophyllum decastes bacterial strain Finc-LD-4 obtaining by domestication, compare with wild strain, cultivation per unit area yield is higher, the in the situation that of same test quantity, Finc-LD-4 has obtained higher gross yield, and total amount of increase in production has reached 30.78%.
Per unit area yield contrast after table 8Finc-LD-4 and FLD-D gather
Embodiment 8 uniformity comparative trials
After Finc-LD-4 and FLD-D are gathered, fruit body is carried out the cap uniformity and stem length consistency analysis, as follows:
As shown in Table 9, Finc-LD-4 bacteria cover diameter average out to 2.6cm, FLD-D bacteria cover diameter average out to 2.8cm, the coefficient of variation of Finc-LD-4 bacteria cover diameter is 33.1%, the coefficient of variation of FLD-D bacteria cover diameter is 37.5%, the relative variability degree of visible Finc-LD-4 bacteria cover diameter is less, illustrates that the bacteria cover diameter of Finc-LD-4 is more even.
The different Lyophyllum decastes bacterial strain of the table 9 fruit body cap size uniformity
Table 10 shows, Finc-LD-4 stem length average out to 9.2cm, FLD-D stem length average out to 8.9cm, the coefficient of variation of Finc-LD-4 stem length is 22.2%, the coefficient of variation of FLD-D stem length is 36.6%, and the relative variability degree of visible Finc-LD-4 stem length is less, and this explanation Finc-LD-4 stem length of the present invention is more even, the mushroom handle length uniformity of Dan Duogu body is better, instant packed.
The long uniformity of the different Lyophyllum decastes bacterial strain of table 10 fruit body stem
Embodiment 9 ITS order-checkings are identified
The present embodiment student on commission work biotechnology (Shanghai) limited company (hereinafter to be referred as " raw work ") carries out, and comprises the following steps:
Genomic extraction: extract according to raw work SK1375 fungal genomic DNA extraction agent box specification.Resulting DNA solution is placed in to-20 ℃ of preservations, standby.
Pcr amplification system is: cumulative volume 25 μ L, genomic templates 1 μ L, fungi rDNA universal primer ITS1 sequence is 5'-TCCGTAGGTGAACCTGCGG-3'(10 μ M) 0.5 μ L, ITS4 sequence is 5'-TCCTCCGCTTATTGATATGC-3'(10 μ M) 0.5 μ L, dNTP mix (10mM each) 0.5 μ L, 10 * Taq reaction buffer, 2.5 μ L, 5U/ μ L Taq0.2 μ L, adds water to 25 μ L.PCR program: 94 ℃ of 5mim of denaturation, 94 ℃ of 30s, 55 ℃ of 35s, 72 ℃ of 1min, 35 circulations, 72 ℃ are extended 8min.
Adopt conventional sequence measurement to check order to above-mentioned pcr amplification product.The ITS sequence sequencing result of this test strain Finc-LD-3 refers to sequence table, the DNA fragmentation that its sequence is 691bp.From GenBank, check in, Finc-LD-4 bacterial strain ITS sequence is the highest with the ITS sequence similarity that belongs to Lyophyllum decastes from pleat umbrella., check in and the ITS sequence of planting close to pleat umbrella symbolic animal of the birth year from GenBank, application DNAStar MegAlign software building goes out the phylogenetic tree of strains tested, refers to accompanying drawing 2 meanwhile.
More than describing is only embodiments of the invention, forgives and can understand, and is not departing under the prerequisite of the present invention's design, within all should being included in technical conceive of the present invention to simple modification of the present invention and replacement.
SEQUENCE LISTING
<110> Shanghai Finc Bio-tech Inc.
<120> Lyophyllum decastes bacterial strain-4
<130>ITS sequence
<160>1
<170>PatentIn version3.3
<210>1
<211>691
<212>DNA
<213>Lyophyllum shimeji
<400>1
tccgtaggtg aacctgcgga aggatcatta ttgaataaat ttggctgggc tgttgctggc 60
tcctaggagt atgtgcacgc ctagctccat tttttaccac ctgtgcacct tttgtagacc 120
tggatatctt tcgaggaaac tcggtttgag gactgcggag cgaaagccct agctttcctt 180
acatttccgg tctatgtctt tacatacccc atatgaatgt aacagaatgt cgtttactgg 240
cctttgtgcc tttaatcaaa tacaactttc agcaacggat ctcttggctc tcgcatcgat 300
gaagaacgca gcgaaatgcg ataagtaatg tgaattgcag aattcagtga atcatcgaat 360
ctttgaacgc acctggcgct ccctggtatt ccggggagca tgtctgtttg agtgtcatta 420
aattctcaac ctttccaact tttgcgagtt tggttaggct tggatgtggg gggttgcggg 480
cttcacagaa gtcggctcct ctgaaatgca ttagtgaaac ctttgttgat catctcttgg 540
tgtgataatt atctacgcca ctagagtgtc agcctcaact tagttgagag gtttttgctt 600
ctaatcgtcc tccaaggaca acttctttac tttttgacct caaatcagat aagactaccc 660
gctgaactta agcatatcaa taagcggagg a691
Claims (1)
1. a Lyophyllum decastes bacterial strain Finc-LD-4, its deposit number is CCTCC NO:M2013008.
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| CN104350954B (en) * | 2014-02-22 | 2016-08-31 | 江苏菇本堂生物科技股份有限公司 | Cornu Cervi Pantotrichum mushroom cultivation method |
| CN105230342B (en) * | 2015-10-22 | 2018-03-06 | 上海丰科生物科技股份有限公司 | Lyophyllum decastes bacterial strain and its cultural method and application |
| CN105210676B (en) * | 2015-10-29 | 2018-03-02 | 青岛丰科生物科技有限公司 | Lyophyllum decastes bacterial strain Finc L6 and its cultural method and application |
| CN111264303B (en) * | 2020-04-09 | 2022-05-06 | 中华全国供销合作总社昆明食用菌研究所 | Lyophyllum fumosoroseum cultivar and preparation method thereof |
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| JPH04330137A (en) * | 1991-01-30 | 1992-11-18 | Aoki Corp | Aseismatic wall structure |
| JP3512899B2 (en) * | 1994-03-17 | 2004-03-31 | タカラバイオ株式会社 | Artificial cultivation method of Hatake Shimeji |
| JP4033760B2 (en) * | 2002-11-20 | 2008-01-16 | タカラバイオ株式会社 | Mushroom cultivation method and mushroom cultivation equipment |
| CN101449649B (en) * | 2007-11-29 | 2011-05-11 | 上海丰科生物科技股份有限公司 | Foundation seed production method of Lyophyllum decastes |
| CN101463324A (en) * | 2007-12-18 | 2009-06-24 | 上海丰科生物科技股份有限公司 | Method for reducing pollution rate of Lyophyllum decastes |
| CN101462898B (en) * | 2007-12-18 | 2012-09-19 | 上海丰科生物科技股份有限公司 | Main ingredient of Lyophyllum decastes cultivation medium |
| KR101234162B1 (en) * | 2010-11-29 | 2013-02-18 | 이대진 | Culture composition and cultivation method for lyophyllumdecastes |
| CN102204475B (en) * | 2011-03-29 | 2012-07-11 | 卢凤忠 | Lyophyllum connatum(schum.txFr.) Sing js39 strain and use thereof |
| CN102907252A (en) * | 2011-08-05 | 2013-02-06 | 魏生龙 | Pilot-scale test fermentation process of mycelia of lyophyllum decastes |
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