CN106613336A - Lucid ganoderma planting method - Google Patents
Lucid ganoderma planting method Download PDFInfo
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- CN106613336A CN106613336A CN201611052200.7A CN201611052200A CN106613336A CN 106613336 A CN106613336 A CN 106613336A CN 201611052200 A CN201611052200 A CN 201611052200A CN 106613336 A CN106613336 A CN 106613336A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention relates to a lucid ganoderma planting method. The lucid ganoderma planting method comprises the steps of culture medium preparing, strain making, fungus stick material making, inoculating, hypha culturing, underwood planting and fungus bud removing. The best raw material is prepared for the next step by conducting careful operation in every step, high-quality fungus sticks which are most suitable for large-scale planting are obtained through stepped optimization and scale expansion, and then lucid ganoderma is high in quality and yield.
Description
Technical field
The present invention relates to a kind of implantation methods, particularly glossy ganoderma implantation methods.
Background technology
Glossy ganoderma is rare Chinese medicine, needs the implantation methods of the constantly good quality and high output of exploration glossy ganoderma.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of glossy ganoderma implantation methods.
Specifically, the technical scheme of present invention offer is：A kind of glossy ganoderma implantation methods, including step：
A, culture medium are prepared, and every 200 g potato matches somebody with somebody 20 grams of glucose, 4 grams of dusty yeast, 2 grams of potassium dihydrogen phosphate, magnesium sulfate 0.5
Gram, 20 grams of agar；Weigh peeling, section 200 grams of potato, be placed in pot, add 1000 milliliters of water, boil to potato chips it is soft and not
It is rotten；With 4-6 layer filtered through gauze, filtrate is taken；20 grams of agar is added in filtrate, is continued to boil to fat and is melted completely, add grape
20 grams of sugar, moisturizing is to 1000 milliliters；It is sub-packed in while hot in test tube, is filled to 1/ 5-1/ 4 of test tube length, cleans the mouth of pipe, beyond the Great Wall
Lid, keeps sealing；Then sterilize 30 minutes under hot conditions；Sterilizing is retreaded and puts test tube, is cultivated 3 days under being placed in 30 degrees Celsius,
If occurring without miscellaneous bacteria, show that sterilizing is thorough, be available for inoculation to use, if miscellaneous bacteria occur and should again sterilize or reconfigure；
B, bacterial classification make, and are divided into ripe fresh sesame and are done lucidum seed from the 6-7 of no disease and pests harm, and lucidum seed surface Jing ultraviolet irradiations disappear
Poison, alcohol wipe sterilization, then cap is torn, it is divided into two；Select on cap yellow-white vitellarium meat bacteria organization or stem
Meat bacteria organization in the middle part of square cap is to take kind of a position；First meat bacteria organization is carefully cut with aseptic operation knife when taking kind, then with connecing
The fritter meat bacteria organization that pin takes 5 millimeters × 5 millimeters is planted, Tube propagation base middle and upper part surface is inoculated into；Bacterial context group is put into by above-mentioned
The culture medium knitted is placed in biochemical cultivation case and cultivates, and to 28-30 degree Celsius, lucifuge culture 2-3 days, meat bacteria organization starts to sprout for temperature adjustment
Send out, macroscopic mycelium occur, and gradually spread in media surface, 7-10 days mycelium cover with test tube slant, mycelia
Body is pure white, very thin, flat, fine and close, uniform, obtains the healthy Ganoderma lucidum mycelium without miscellaneous bacteria, with the growth of mycelia, the expansion of bacterium colony
Exhibition, the mycelia at inoculation is gradually aging to form tough and tensile mycoderma, and as mycoderma is aging, at this time selects Ganoderma lucidum mycelium bulk-growth
Neatly, long speed is normal, mycelia outward appearance is full, growing way is vigorous, and aerial hyphae is pure white, very thin, flat, fine and close, uniform part is made
For bacterial classification；
C, bacterium rod make of material, select the seeds for being adapted to cultivation basswood, sawed-off for length 14-15 centimetre in 10 to 20 days after felling
Or 28-30 centimetre, a diameter of 3-16 centimetre, the short linden that section is smooth, length is consistent is used as bacterium rod material；From high temperature resistant,
The packed bacterium rod material of polyethylene cylinder of stretching resistance, slip-knot is pricked respectively at two after pack, is handled with care, prevents breakage；Will be packed
Bacterium rod material is put into high-temperature sterilization in pot, and very hot oven onslaught, makes kettle temperature be rapidly reached 100 degrees Celsius after being put in pot, works as pot
To maintain do not lower the temperature after 100 degrees Celsius of Nei Wenduda within 12 hours, taking the dish out of the pot after truce is cooled to 28-30 degree Celsius；
D, inoculation, transfer room can select good doors and windows sealing, place drying, 10-15 square metre of room of cleaning, interior to use plastic foil system
Into equal inoculation account, 4-8 hours are carried out disinfection per cubic metre of account is inoculated with disinfectant before inoculation, and bacterial classification is filled into bacterium
Rod with bacterium rod is obtained after the section of material two, wrap and tying is stacked by bacterium rod plastic foil；
E, cultural hypha, select the cob wall room of good heat insulating as between culture, and room temperature is controlled at 20-30 degree Celsius；Culture
Between want lucifuge, illumination is suitably increased when mycelia covers with full bag, promotes mycelial growth, 60-70 days mycelia of culture are ripe, bacterium rod table
Layer mycelia is pure white sturdy, is close between bacterium wood and is difficult to break into two with one's hands, and minority bacterium wood section has the big former base of beans to occur, you can carry out sylvan life kind
Plant；
F, underwood planting, selected from right pavilion leaf woods, it is desirable to seven parts of the moon of sylvan life, three parts of sun, can grow be used for where wild weeds on the ground
Glossy ganoderma underwood planting, 4-5 hole of often row digging, cheats away from 20 centimeters, and bacterium rod is stood up in hole, equal with ground, upper cover 1-2 public affairs
The soil for dividing, the place of cultivating ganoderma keeps weed growth, Planting Grass or mowing capping when weeds are very few；
G, go button, to be unclogged and readjusted when button grows two centimeters high, unnecessary button is removed, go it is weak stay strong, per rod only
Retain 1-2 button.
Further technical scheme is the present invention：What the bacterium rod material was selected is the maple for bending many knots.
The present invention obtain beneficial effect be：It is that next step makes best raw material by the carefulness operation to each step
And preparation, by step-by-step optimization and expansion scale, obtain the high-quality bacterium rod of optimum implant mass so that glossy ganoderma high-quality is high
Produce.
Description of the drawings
Fig. 1 is the plantation flow chart of glossy ganoderma of the present invention.
Specific embodiment
As shown in figure 1, a kind of glossy ganoderma implantation methods, are that next step making is best by the carefulness operation to each step
Raw material and preparation, by step-by-step optimization and expansion scale, obtain the high-quality bacterium rod of optimum implant mass so that glossy ganoderma high-quality
High yield, concrete scheme includes step：
A, culture medium are prepared, and every 200 g potato matches somebody with somebody 20 grams of glucose, 4 grams of dusty yeast, 2 grams of potassium dihydrogen phosphate, magnesium sulfate 0.5
Gram, 20 grams of agar；Weigh peeling, section 200 grams of potato, be placed in pot, add 1000 milliliters of water, boil to potato chips it is soft and not
It is rotten；With 4-6 layer filtered through gauze, filtrate is taken；20 grams of agar is added in filtrate, is continued to boil to fat and is melted completely, add grape
20 grams of sugar, moisturizing is to 1000 milliliters；It is sub-packed in while hot in test tube, is filled to 1/ 5-1/ 4 of test tube length, cleans the mouth of pipe, beyond the Great Wall
Lid, keeps sealing；Then sterilize 30 minutes under hot conditions；Sterilizing is retreaded and puts test tube, is cultivated 3 days under being placed in 30 degrees Celsius,
If occurring without miscellaneous bacteria, show that sterilizing is thorough, be available for inoculation to use, if miscellaneous bacteria occur and should again sterilize or reconfigure.
B, bacterial classification make, and are divided into ripe fresh sesame and are done lucidum seed, the ultraviolet photographs of lucidum seed surface Jing from the 6-7 of no disease and pests harm
Sterilization, alcohol wipe sterilization are penetrated, then cap is torn, be divided into two；Select cap yellow-white vitellarium meat bacteria organization or bacterium
Meat bacteria organization in the middle part of the cap of handle top is to take kind of a position；First meat bacteria organization is carefully cut with aseptic operation knife when taking kind, then
5 millimeters × 5 millimeters of fritter meat bacteria organization is taken with transfer needle, Tube propagation base middle and upper part surface is inoculated into；Bacterium is put into by above-mentioned
The culture medium of meat tissue is placed in biochemical cultivation case and cultivates, temperature adjustment to 28-30 degree Celsius, lucifuge culture 2-3 days, meat bacteria organization opens
Begin to sprout, macroscopic mycelium occur, and gradually spread in media surface, mycelium covers with test tube slant within 7-10 days,
Mycelium is pure white, very thin, flat, fine and close, uniform, obtains the healthy Ganoderma lucidum mycelium without miscellaneous bacteria, the growth, bacterium colony with mycelia
Extension, the mycelia at inoculation is gradually aging to form tough and tensile mycoderma, and as mycoderma is aging, at this time selects ganoderma lucidum mycelium
Growth is neat, long speed is normal, mycelia outward appearance is full, growing way is vigorous, and aerial hyphae is pure white, very thin, flat, fine and close, uniform portion
It is allocated as bacterial classification.If separator（Meat bacteria organization）Macroscopic mycelium, eventful miscellaneous bacteria were occurred as soon as less than 2 days.If point
From thing（Meat bacteria organization）Culture is still the macroscopic mycelium of appearance in 4 days, it may be possible to cut the scalpel of glossy ganoderma meat bacteria organization
Or transfer needle is no sufficiently cool after calcination above Alcohol Flame, or other reasons cause separator（Meat bacteria organization）Lose
Deactivation and mycelium can not be sprouted.
C, bacterium rod make of material, select the seeds for being adapted to cultivation basswood, sawed-off for length 14-15 in 10 to 20 days after felling
Centimetre or 28-30 centimetre, a diameter of 3-16 centimetre, the short linden that section is smooth, length is consistent is used as bacterium rod material；From resistance to height
Temperature, the packed bacterium rod material of polyethylene cylinder of stretching resistance, slip-knot is pricked respectively at two after pack, is handled with care, prevents breakage；Will be packed
Bacterium rod material be put into high-temperature sterilization in pot, be put in pot after very hot oven onslaught, make kettle temperature be rapidly reached 100 degrees Celsius, when
Kettle temperature will maintain do not lower the temperature for 12 hours up to after 100 degrees Celsius, and taking the dish out of the pot after truce is cooled to 28-30 degree Celsius；Preferably, institute
State the selection of bacterium rod material is the maple for bending many knots.
D, inoculation, transfer room can select good doors and windows sealing, place drying, 10-15 square metre of room of cleaning, inside use plastics
Film makes equal inoculation account, and 4-8 hours are carried out disinfection per cubic metre of account is inoculated with disinfectant before inoculation, and bacterial classification is filled
To bacterium rod with bacterium rod is obtained after the section of material two, bacterium rod plastic foil is wrapped and tying is stacked.
E, cultural hypha, select the cob wall room of good heat insulating as between culture, and room temperature is controlled at 20-30 degree Celsius；
Lucifuge is wanted between culture, illumination is suitably increased when mycelia covers with full bag, is promoted mycelial growth, 60-70 days mycelia of culture are ripe, bacterium
Mycelia is pure white sturdy on rod top layer, is close between bacterium wood and is difficult to break into two with one's hands, and minority bacterium wood section has the big former base of beans to occur, you can carry out woods
Lower plantation.
F, underwood planting, selected from right pavilion leaf woods, it is desirable to seven parts of the moon of sylvan life, three parts of sun, where wild weeds can be grown on the ground
For glossy ganoderma underwood planting, 4-5 hole of often row digging, cheat away from 20 centimeters, bacterium rod is stood up in hole, upper cover equal with ground
The soil of 1-2 centimetres, the place of cultivating ganoderma keeps weed growth, Planting Grass or mowing capping when weeds are very few；Allow spirit
Sesame grows out the glossy ganoderma shape that just keep from grass, can just keep Ganoderma Lucidum pin height, and can prevent rain from beating mud damage
Glossy ganoderma bottom surface, if speckling with mud on Ganoderma Lucidum, bottom just has pit, will affect quality.
G, go button, to be unclogged and readjusted when button grows two centimeters high, unnecessary button is removed, go it is weak stay strong, often
Rod only retains 1-2 button.Because glossy ganoderma can not draw nutrient from soil, for its growth, it can only draw wood from timber
Quality is supplying growth, that is to say, that its nutrient source of Ganoderma Lucidum is limited, so reservation is more such as on same bacterium rod
Little is reformed into if individual glossy ganoderma, commodity value can be thus affected.
Specific embodiment be it is exemplary present invention is described, be not intended to limit the present invention be included in right will
Seek interior other embodiment.
Claims (2)
1. a kind of glossy ganoderma implantation methods, it is characterised in that including step：
A, culture medium are prepared, and every 200 g potato matches somebody with somebody 20 grams of glucose, 4 grams of dusty yeast, 2 grams of potassium dihydrogen phosphate, magnesium sulfate 0.5
Gram, 20 grams of agar；Weigh peeling, section 200 grams of potato, be placed in pot, add 1000 milliliters of water, boil to potato chips it is soft and not
It is rotten；With 4-6 layer filtered through gauze, filtrate is taken；20 grams of agar is added in filtrate, is continued to boil to fat and is melted completely, add grape
20 grams of sugar, moisturizing is to 1000 milliliters；It is sub-packed in while hot in test tube, is filled to 1/ 5-1/ 4 of test tube length, cleans the mouth of pipe, beyond the Great Wall
Lid, keeps sealing；Then sterilize 30 minutes under hot conditions；Sterilizing is retreaded and puts test tube, is cultivated 3 days under being placed in 30 degrees Celsius,
If occurring without miscellaneous bacteria, show that sterilizing is thorough, be available for inoculation to use, if miscellaneous bacteria occur and should again sterilize or reconfigure；
B, bacterial classification make, and are divided into ripe fresh sesame and are done lucidum seed from the 6-7 of no disease and pests harm, and lucidum seed surface Jing ultraviolet irradiations disappear
Poison, alcohol wipe sterilization, then cap is torn, it is divided into two；Select on cap yellow-white vitellarium meat bacteria organization or stem
Meat bacteria organization in the middle part of square cap is to take kind of a position；First meat bacteria organization is carefully cut with aseptic operation knife when taking kind, then with connecing
The fritter meat bacteria organization that pin takes 5 millimeters × 5 millimeters is planted, Tube propagation base middle and upper part surface is inoculated into；Bacterial context group is put into by above-mentioned
The culture medium knitted is placed in biochemical cultivation case and cultivates, and to 28-30 degree Celsius, lucifuge culture 2-3 days, meat bacteria organization starts to sprout for temperature adjustment
Send out, macroscopic mycelium occur, and gradually spread in media surface, 7-10 days mycelium cover with test tube slant, mycelia
Body is pure white, very thin, flat, fine and close, uniform, obtains the healthy Ganoderma lucidum mycelium without miscellaneous bacteria, with the growth of mycelia, the expansion of bacterium colony
Exhibition, the mycelia at inoculation is gradually aging to form tough and tensile mycoderma, and as mycoderma is aging, at this time selects Ganoderma lucidum mycelium bulk-growth
Neatly, long speed is normal, mycelia outward appearance is full, growing way is vigorous, and aerial hyphae is pure white, very thin, flat, fine and close, uniform part is made
For bacterial classification；
C, bacterium rod make of material, select the seeds for being adapted to cultivation basswood, sawed-off for length 14-15 centimetre in 10 to 20 days after felling
Or 28-30 centimetre, a diameter of 3-16 centimetre, the short linden that section is smooth, length is consistent is used as bacterium rod material；From high temperature resistant,
The packed bacterium rod material of polyethylene cylinder of stretching resistance, slip-knot is pricked respectively at two after pack, is handled with care, prevents breakage；Will be packed
Bacterium rod material is put into high-temperature sterilization in pot, and very hot oven onslaught, makes kettle temperature be rapidly reached 100 degrees Celsius after being put in pot, works as pot
To maintain do not lower the temperature after 100 degrees Celsius of Nei Wenduda within 12 hours, taking the dish out of the pot after truce is cooled to 28-30 degree Celsius；
D, inoculation, transfer room can select good doors and windows sealing, place drying, 10-15 square metre of room of cleaning, interior to use plastic foil system
Into equal inoculation account, 4-8 hours are carried out disinfection per cubic metre of account is inoculated with disinfectant before inoculation, and bacterial classification is filled into bacterium
Rod with bacterium rod is obtained after the section of material two, wrap and tying is stacked by bacterium rod plastic foil；
E, cultural hypha, select the cob wall room of good heat insulating as between culture, and room temperature is controlled at 20-30 degree Celsius；Culture
Between want lucifuge, illumination is suitably increased when mycelia covers with full bag, promotes mycelial growth, 60-70 days mycelia of culture are ripe, bacterium rod table
Layer mycelia is pure white sturdy, is close between bacterium wood and is difficult to break into two with one's hands, and minority bacterium wood section has the big former base of beans to occur, you can carry out sylvan life kind
Plant；
F, underwood planting, selected from right pavilion leaf woods, it is desirable to seven parts of the moon of sylvan life, three parts of sun, can grow be used for where wild weeds on the ground
Glossy ganoderma underwood planting, 4-5 hole of often row digging, cheats away from 20 centimeters, and bacterium rod is stood up in hole, equal with ground, upper cover 1-2 public affairs
The soil for dividing, the place of cultivating ganoderma keeps weed growth, Planting Grass or mowing capping when weeds are very few；
G, go button, to be unclogged and readjusted when button grows two centimeters high, unnecessary button is removed, go it is weak stay strong, per rod only
Retain 1-2 button.
2. glossy ganoderma implantation methods as claimed in claim 1, it is characterised in that：What the bacterium rod material was selected is many knots of bending
Maple.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611052200.7A CN106613336A (en) | 2016-11-25 | 2016-11-25 | Lucid ganoderma planting method |
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|---|---|---|---|
| CN201611052200.7A CN106613336A (en) | 2016-11-25 | 2016-11-25 | Lucid ganoderma planting method |
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Family
ID=58811223
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| CN201611052200.7A Pending CN106613336A (en) | 2016-11-25 | 2016-11-25 | Lucid ganoderma planting method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107484548A (en) * | 2017-08-07 | 2017-12-19 | 霍山县永丰蚕业农民专业合作社 | A kind of ganoderma lucidum cultivation method for improving ganoderma polyoses content |
| CN110122175A (en) * | 2019-06-12 | 2019-08-16 | 北京葆年堂(菏泽)药业有限公司 | A kind of implantation methods of ganoderma lucidum |
| CN110521488A (en) * | 2019-09-24 | 2019-12-03 | 广西壮族自治区林业科学研究院 | A method of Ganoderma Sinense is cultivated using forest felling stub field original place |
| CN112088718A (en) * | 2019-06-17 | 2020-12-18 | 米林县米林镇红太阳科技示范家庭农场 | Preparation method of milin ganoderma lucidum strain |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107484548A (en) * | 2017-08-07 | 2017-12-19 | 霍山县永丰蚕业农民专业合作社 | A kind of ganoderma lucidum cultivation method for improving ganoderma polyoses content |
| CN110122175A (en) * | 2019-06-12 | 2019-08-16 | 北京葆年堂(菏泽)药业有限公司 | A kind of implantation methods of ganoderma lucidum |
| CN112088718A (en) * | 2019-06-17 | 2020-12-18 | 米林县米林镇红太阳科技示范家庭农场 | Preparation method of milin ganoderma lucidum strain |
| CN110521488A (en) * | 2019-09-24 | 2019-12-03 | 广西壮族自治区林业科学研究院 | A method of Ganoderma Sinense is cultivated using forest felling stub field original place |
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Application publication date: 20170510 |