CN107711285A - A kind of method of culture bottle factory culture Hericium erinaceus - Google Patents
A kind of method of culture bottle factory culture Hericium erinaceus Download PDFInfo
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- CN107711285A CN107711285A CN201710971073.9A CN201710971073A CN107711285A CN 107711285 A CN107711285 A CN 107711285A CN 201710971073 A CN201710971073 A CN 201710971073A CN 107711285 A CN107711285 A CN 107711285A
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- hericium erinaceus
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- flower bud
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- Inorganic Chemistry (AREA)
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Abstract
A kind of method of culture bottle factory culture Hericium erinaceus, this method are carried out factory culture using culture bottle as cultivating container, comprised the following steps：（1）Prepare culture medium,（2）The filling culture medium into culture bottle,（3）Sterilized to culture bottle,（4）It is inoculated with into blake bottle,（5）Cultural hypha,（6）Flower bud,（7）First tide harvesting,（8）Second tide harvesting.The present invention is advantageously implemented batch production and mechanization, and Hericium erinaceus growing environment is easily controlled, and Hericium erinaceus shape is close consistent and attractive in appearance.
Description
Technical field
The present invention relates to a kind of method of culture bottle factory culture Hericium erinaceus, belongs to edible fungus industrial cultivation technology neck
Domain.
Background technology
Hericium erinaceus is called Hericium erinaceus, monkey mushroom, hedgehog hydnum, is the precious edible medicinal treasure in China, because being burst with alleviation alimentary canal
Ulcer, chronic gastritis effect are loved by consumers.Wild Hericium erinaceus is grown in the broad leaf tree rotten wood or standing tree of remote, thickly forested mountains more
Injury, domestic Hericium erinaceus domesticating and cultivating have had the history of more than 30 years, are mainly trained at present using polybag as vessel filling
Nutriment is made long bacteria stick and carries out artificial substituting stuff cultivation, traditional peasant household's cultivation stage is remained in, because not using machine on a large scale
Tool, production efficiency is relatively low, while production is influenceed also larger, therefore the production scale of Hericium erinaceus by natural climate and pest and disease damage
It can not expand, cause to hold at high price.
With health and the popularization of dietotherapy theory and the excavation of Hericium erinaceus nutritive value, to the demand of Hericium erinaceus increasingly
Greatly, but traditional single household's formula cultivation can not meet the growth of consumption, therefore exploitation cultivation period is short, and production efficiency is high, holds
The production model being easily widely applied turns into the task of top priority.
The cultivation of current Hericium erinaceus also mainly uses pocket type cultivation, it is difficult to control the growth form of Hericium erinaceus so that monkey
Head mushroom grows different, it is impossible to meets the vision requirement of consumer；Meanwhile pocket type cultivation internal environment is unstable, need
Perforate to realize that inner ventilation is taken a breath on sack, and sack is easily deformed, adjacent bags easily mutually extrude.
The content of the invention
In order to solve above-mentioned problem, the invention discloses a kind of method of culture bottle factory culture Hericium erinaceus,
Concrete technical scheme is as follows：
A kind of method of culture bottle factory culture Hericium erinaceus, this method carry out batch production cultivation using culture bottle as cultivating container
Training, comprises the following steps：
Step（1）：Prepare culture medium
Raw material cotton seed hulls, corncob, wood chip, wheat bran and land plaster are weighed, puts it into mixer and is stirred, whipping process
It is middle to continue stirring to each component and be well mixed into raw material plus water, form culture medium, and final culture medium moisture content be 60%~
65%；
Step（2）：Filling culture medium
By step（1）The culture medium prepared is filled in culture bottle by automatic bottling machine, while is punched in the medium, lid
Upper cultivation bottle cap；
Step（3）：Sterilizing
Step will be passed through（2）The culture bottle for installing culture medium is put into autoclave and carries out high pressure steam sterilization, kills in culture medium
Microorganism and its brood body；
Step：（4）Inoculation
Step will be passed through（3）Culture bottle after sterilizing is placed in desinfection chamber, naturally cools to 18~22 DEG C, opens cultivation bottle cap,
Strain is accessed into culture bottle with automatic vaccination machine, then covers cultivation bottle cap again；
Step：（5）Cultural hypha
Step will be passed through（4）Culture bottle after inoculation is placed on temperature as 22~25 DEG C, and air humidity is 60%~70% culture
Indoor culture, mycelia covers with culture bottle within 20~25 days；
Step：（6）Flower bud
By step（5）The culture bottle of gained removes cultivation bottle cap, then covers fruiting cap for special purpose, is placed on temperature as 16~20
DEG C, air humidity is 85%~95% fertility interior carry out flower bud, after mushroom flower bud grows, gives mushroom flower bud illumination, promotes the life of mushroom flower bud
It is long；
Step：（7）First tide harvesting
Mushroom flower bud is grown 10~15 days afterwards, and Hericium erinaceus is harvested：
Step：（8）Second tide harvesting
After first tide harvesting, according to step（6）Flower bud is carried out again：First moisture knot beam 15~20 days, the second tide harvesting.
The step（1）Middle cotton seed hulls, corncob, wood chip, the mass fraction of wheat bran and land plaster are cotton seed hulls 30~40
Part, 20~30 parts of corncob, 20~30 parts of wood chip, 10~15 parts of wheat bran, 1~2 part of land plaster.
The cultivation bottle cap is provided with air vent, cultivates and cavity is provided with bottle cap, and air vent is respectively positioned at cultivation bottle cap
Outside surrounding and inside circumference, and connected with cavity.
The fruiting hole for being provided centrally with growing for Hericium erinaceus of the fruiting cap for special purpose.
The step（3）The temperature of mesohigh steam sterilizing is 121 DEG C, sterilizing air pressure is 0.120-0.130 MPa, and held
90~120min of continuous sterilizing.
The step（6）Middle flower bud uses the illumination during growth of mushroom flower bud, and for blue violet light, intensity of illumination is 200~400Lux.
The step（2）The depth punched in the medium is the bottom from the upper surface of culture medium to culture medium.
The beneficial effects of the invention are as follows：
The present invention uses cultivating container of the culture bottle as Hericium erinaceus, because the shape of culture bottle is fixed, more traditional cultivating bag,
Culture medium can more advantageously be loaded.Culture bottle is covered with passage, is easy to ventilation in culture bottle, while air vent will be through
The cavity for crossing cultivation bottle cap changes gas, and cavity has cushioning effect to gas, and gas, which is changed, inside and outside culture bottle relaxes, and will not produce
Raw big air-flow, meanwhile, cavity has the function that to filter and precipitated to the gas for entering culture bottle, avoids the miscellaneous bacteria in air from entering
Enter into culture bottle.
Because culture bottle is the bottle of hard, cultivate bottle cap and fruiting cap for special purpose can mechanically install and
Remove, be advantageously implemented mechanized operation.And culture bottle can be put successively on cultivating stand, area occupied, any phase are saved
Adjacent culture bottle will not influence each other.
By punching in the medium, meet the oxygen demand in culture medium, the inner space of culture bottle is relatively fixed, favorably
In the growing environment for keeping stable Hericium erinaceus.
Hericium erinaceus from fruiting cap for special purpose grow, can trained shape close to consistent Hericium erinaceus profile, meet to disappear
Demand of the person of expense for the good appearance of mushroom class.
Embodiment
With reference to embodiment, the present invention is furture elucidated.It should be understood that following embodiments are only used for
The bright present invention rather than limitation the scope of the present invention.
A kind of method of culture bottle factory culture Hericium erinaceus, this method carry out batch production using culture bottle as cultivating container
Cultivation, comprises the following steps：
Step（1）：Prepare culture medium
Raw material cotton seed hulls, corncob, wood chip, wheat bran and land plaster are weighed, puts it into mixer and is stirred, whipping process
It is middle to continue stirring to each component and be well mixed into raw material plus water, form culture medium, and final culture medium moisture content be 60%~
65%；
Step（2）：Filling culture medium
By step（1）The culture medium prepared is filled in culture bottle by automatic bottling machine, while is punched in the medium, lid
Upper cultivation bottle cap；
Step（3）：Sterilizing
Step will be passed through（2）The culture bottle for installing culture medium is put into autoclave and carries out high pressure steam sterilization, kills in culture medium
Microorganism and its brood body；
Step：（4）Inoculation
Step will be passed through（3）Culture bottle after sterilizing is placed in desinfection chamber, naturally cools to 18~22 DEG C, opens cultivation bottle cap,
Strain is accessed into culture bottle with automatic vaccination machine, then covers cultivation bottle cap again；
Step：（5）Cultural hypha
Step will be passed through（4）Culture bottle after inoculation is placed on temperature as 22~25 DEG C, and air humidity is 60%~70% culture
Indoor culture, mycelia covers with culture bottle within 20~25 days；
Step：（6）Flower bud
By step（5）The culture bottle of gained removes cultivation bottle cap, then covers fruiting cap for special purpose, is placed on temperature as 16~20
DEG C, air humidity is 85%~95% fertility interior carry out flower bud, after mushroom flower bud grows, gives mushroom flower bud illumination, promotes the life of mushroom flower bud
It is long；
Step：（7）First tide harvesting
Mushroom flower bud is grown 10~15 days afterwards, and Hericium erinaceus is harvested：
Step：（8）Second tide harvesting
After first tide harvesting, according to step（6）Flower bud is carried out again：First moisture knot beam 15~20 days, the second tide harvesting.
The step（1）Middle cotton seed hulls, corncob, wood chip, the mass fraction of wheat bran and land plaster are cotton seed hulls 30~40
Part, 20~30 parts of corncob, 20~30 parts of wood chip, 10~15 parts of wheat bran, 1~2 part of land plaster.
The cultivation bottle cap is provided with air vent, cultivates and cavity is provided with bottle cap, and air vent is respectively positioned at cultivation bottle cap
Outside surrounding and inside circumference, and connected with cavity.
The fruiting hole for being provided centrally with growing for Hericium erinaceus of the fruiting cap for special purpose.
The step（3）The temperature of mesohigh steam sterilizing is 121 DEG C, sterilizing air pressure is 0.120-0.130 MPa, and held
90~120min of continuous sterilizing.
The step（6）Middle flower bud uses the illumination during growth of mushroom flower bud, and for blue violet light, intensity of illumination is 200~400Lux.
The step（2）The depth punched in the medium is the bottom from the upper surface of culture medium to culture medium.
The present invention is in embodiment, and the first average every bottle of yield of tide harvesting is 154.4g, cultivating rate 97%；Second tide is adopted
It is 112.9g to be closed flat every bottle of yield, cultivating rate 94%；Average total single bottle yield 267.3g.From being inoculated into whole harvestings, cycle
For 54 days.
Technological means disclosed in the present invention program is not limited only to the technological means disclosed in above-mentioned technological means, in addition to
Formed technical scheme is combined by above technical characteristic.
Claims (7)
1. a kind of method of culture bottle factory culture Hericium erinaceus, this method carry out batch production cultivation using culture bottle as cultivating container
Training, it is characterized in that comprising the following steps：
Step（1）：Prepare culture medium
Raw material cotton seed hulls, corncob, wood chip, wheat bran and land plaster are weighed, puts it into mixer and is stirred, whipping process
It is middle to continue stirring to each component and be well mixed into raw material plus water, form culture medium, and final culture medium moisture content be 60%~
65%；
Step（2）：Filling culture medium
By step（1）The culture medium prepared is filled in culture bottle by automatic bottling machine, while is punched in the medium, lid
Upper cultivation bottle cap；
Step（3）：Sterilizing
Step will be passed through（2）The culture bottle for installing culture medium is put into autoclave and carries out high pressure steam sterilization, kills in culture medium
Microorganism and its brood body；
Step：（4）Inoculation
Step will be passed through（3）Culture bottle after sterilizing is placed in desinfection chamber, naturally cools to 18~22 DEG C, opens cultivation bottle cap,
Strain is accessed into culture bottle with automatic vaccination machine, then covers cultivation bottle cap again；
Step：（5）Cultural hypha
Step will be passed through（4）Culture bottle after inoculation is placed on temperature as 22~25 DEG C, and air humidity is 60%~70% culture
Indoor culture, mycelia covers with culture bottle within 20~25 days；
Step：（6）Flower bud
By step（5）The culture bottle of gained removes cultivation bottle cap, then covers fruiting cap for special purpose, is placed on temperature as 16~20
DEG C, air humidity is 85%~95% fertility interior carry out flower bud, after mushroom flower bud grows, gives mushroom flower bud illumination, promotes the life of mushroom flower bud
It is long；
Step：（7）First tide harvesting
Mushroom flower bud is grown 10~15 days afterwards, and Hericium erinaceus is harvested：
Step：（8）Second tide harvesting
After first tide harvesting, according to step（6）Flower bud is carried out again：First moisture knot beam 15~20 days, the second tide harvesting.
A kind of 2. method of culture bottle factory culture Hericium erinaceus according to claim 1, it is characterized in that the step（1）
Middle cotton seed hulls, corncob, wood chip, the mass fraction of wheat bran and land plaster are 30~40 parts of cotton seed hulls, 20~30 parts of corncob, wood
20~30 parts of bits, 10~15 parts of wheat bran, 1~2 part of land plaster.
A kind of 3. method of culture bottle factory culture Hericium erinaceus according to claim 1, it is characterized in that the culture bottle
Lid is provided with air vent, cultivates and cavity is provided with bottle cap, and air vent is respectively positioned at the outside surrounding and inside four for cultivating bottle cap
Week, and connected with cavity.
A kind of 4. method of culture bottle factory culture Hericium erinaceus according to claim 1, it is characterized in that the fruiting is special
With the fruiting hole for being provided centrally with growing for Hericium erinaceus of bottle cap.
A kind of 5. method of culture bottle factory culture Hericium erinaceus according to claim 1, it is characterized in that the step（3）
The temperature of mesohigh steam sterilizing is 121 DEG C, sterilizing air pressure is 0.120-0.130 MPa, and continues 90~120min of sterilizing.
A kind of 6. method of culture bottle factory culture Hericium erinaceus according to claim 1, it is characterized in that the step（6）
Middle flower bud uses the illumination during growth of mushroom flower bud, and for blue violet light, intensity of illumination is 200~400Lux.
A kind of 7. method of culture bottle factory culture Hericium erinaceus according to claim 1, it is characterized in that the step（2）
The depth punched in the medium is the bottom from the upper surface of culture medium to culture medium.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108541511A (en) * | 2018-04-25 | 2018-09-18 | 贵州高山百益食用菌发展有限公司 | A kind of method of pure Rosa roxburghii Tratt slag for cultivating Hericium erinaceus |
| CN108782008A (en) * | 2018-06-26 | 2018-11-13 | 福建神农菇业股份有限公司 | A method of cultivating edible mushroom using edible fungi cultivating bottle |
| CN110226450A (en) * | 2019-06-12 | 2019-09-13 | 肇庆绿健农业科技有限公司 | A kind of breeding method of the brave matsutake of bottle of cultivation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108541511A (en) * | 2018-04-25 | 2018-09-18 | 贵州高山百益食用菌发展有限公司 | A kind of method of pure Rosa roxburghii Tratt slag for cultivating Hericium erinaceus |
| CN108782008A (en) * | 2018-06-26 | 2018-11-13 | 福建神农菇业股份有限公司 | A method of cultivating edible mushroom using edible fungi cultivating bottle |
| CN110226450A (en) * | 2019-06-12 | 2019-09-13 | 肇庆绿健农业科技有限公司 | A kind of breeding method of the brave matsutake of bottle of cultivation |
| CN110226450B (en) * | 2019-06-12 | 2021-12-28 | 向国忠 | Cultivation method of bottle-cultivated tiger tricholoma matsutake |
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