CN104488544A - Cultivation method of auriailaria polytricha - Google Patents

Cultivation method of auriailaria polytricha Download PDF

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Publication number
CN104488544A
CN104488544A CN201410717916.9A CN201410717916A CN104488544A CN 104488544 A CN104488544 A CN 104488544A CN 201410717916 A CN201410717916 A CN 201410717916A CN 104488544 A CN104488544 A CN 104488544A
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bacteria
bacterium bag
ear
bag
collarium
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CN104488544B (en
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李拴英
王金科
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Baoji Yingzi Fungus Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention relates to a cultivation method of auriailaria polytricha. The cultivation method comprises the following steps: (1) preparing a cultivation material; (2) bagging: arranging bacteria rings at the upper ends of bacteria bags filled with the material in a sleeving way, inserting inoculating sticks into the bacteria rings, pulling out bag mouths of the bacteria bags outwards, arranging on the bacteria rings in a sleeving way and tightening by using rubber bands; (3) performing sterilization under atmospheric pressure; (4) inoculating: pulling out the inoculating sticks to form circular hole columns in the middle of the bacteria bags, injecting strains from the circular hole columns under aseptic conditions and stuffing cotton plugs in the bacteria rings; (5) performing spawn running: filling the bacteria bags with mycelium; (6) cutting: cutting V-shaped openings in the peripheries of the bacteria bags filled with the mycelium; (7) hanging the bags: carrying out bag hanging on an ear tent, tying the bacteria bags on a rope through slipknots in sequence, tying a fast knot on the last bacteria bag at the bacteria ring, tensioning the rope through the dead weight of each bacteria bag so as to ensure that the bacteria bag cannot fall off; (8) performing earing management; (9) harvesting and processing. The cultivation method has the advantages of being small in floor area, good in ventilation, free of cultivation dead angle, high in output and good in quality, facilitates the control of temperature and humidity, reduces cost and saves labor.

Description

A kind of cultivation method of Yellow-back fungus
Technical field
The present invention relates to fungus growing technique field, be specifically related to a kind of cultivation method of Yellow-back fungus.
Background technology
Yellow-back fungus Auricularia polytricha (Mont) Sacl.The torrid zone and one of subtropical zone Pollution In Edible Mushrooms, taxonomy is under the jurisdiction of Basidiomycota, heterobasidium bacterium just, Auriculariale, Yellow-back fungus belong to.It is nutritious, and matter is crisp good to eat, containing 8 seed amino acids that human body is necessary, has medical care effect, be small investment, instant effect, income greatly, extremely potential edible mushroom new varieties, and be popular healthy food.But Yellow-back fungus culture technique difficulty is large, normally goes out ear rate low, easily cause cultivation failure or the underproduction.Trace it to its cause many, wherein crucial technological difficulties do not break the traditional mode of fungus cultivation in the past, i.e. posture fungus cultivation and the earth fungus cultivation pattern.It is high that posture fungus cultivation pattern not only cultivates cost, and difficulty of gathering is large, is not suitable for large-scale production, and humidity is uneven, and ventilating air permeability is poor, easily the lopsided ear of appearance.The earth fungus cultivation pattern is all placed on the ground due to bacterium bag, and easily grow green mold from ear mouth, cause secondary miscellaneous bacteria to infect, surface humidity great Yi causes stream ear and rotten ear phenomenon in addition, and floor space greatly, effectively can not utilize solid space, reduce costs.
Summary of the invention
For solving the problem; the invention provides a kind of cultivation method of Yellow-back fungus, break the traditional mode of posture fungus cultivation and the earth fungus cultivation, adopt factory three-dimensional cultivation mode; floor space is little; without cultivation dead angle, not only reduce cost, to save artificial; and output is high; quality is good, also solves a difficult problem such as Yellow-back fungus children ear phase stream ear, green mold pollution etc., for Yellow-back fungus batch production, scale, industrialization cultivation provide a practicable method simultaneously.
Technical solution of the present invention: a kind of cultivation method of Yellow-back fungus, comprises the steps:
(1) planting material is prepared and poach by following weight ratio: cotton seed hulls 30-40%, maize cob meal 30-40%, weed tree sawdust 30-40%, bran chaff 10-20%, brown sugar 1-2%, calcium carbonate or gypsum 1-2%, superphosphate 1-2%; Water content 55%-60%, pH value is 7-7.5;
(2) pack: the packed siccative 500-550 gram of each bacterium, and put collarium by after the bacterium bag upper end reducing installing planting material, then in collarium, loop-carrier is inserted, and make loop-carrier be positioned at bacterium bag central authorities, again bacterium bag sack is outwards routed up the outside being enclosed within collarium, and with rubber band, bacterium bag sack is tied tight on collarium;
(3) sterilizing: normal-pressure sterilization temperature 100 DEG C, continues 24-25 hour;
(4) inoculate: when the bacterium bag after sterilizing is cooled to below 25 DEG C, extract loop-carrier, make bacterium bag central authorities form a circular port post, then aseptically adopt inoculating gun to be injected from circular port post by Yellow-back fungus bacterial classification, and in collarium, fill in tampon close sack;
(5) bacteria: postvaccinal bacterium bag wall-is deposited in and sends out bacterium room, cultivate under 23 DEG C of-25 DEG C of constant temperature and send out bacterium, incubation time 40 ~ 48 days, makes mycelia cover with bacterium bag;
(6) designated port: after bacterium bag is immersed disinfecting solution of potassium permanganate process, covering with the bacterium bag surrounding of mycelia, drawing " V " shape mouth 10-12 with sterile blade, wall-stacks 5-7 days, and covers straw screen or mat, prepares Pig after knife-edge part mycelia is recovered;
(7) Pig: carry out Pig at ear canopy, string spacing 10 ~ 15 centimeters during Pig, bag spacing 3 ~ 5 centimeters; Often string only needs a rope, rope upper end is fixed on ear ceiling portion, then left hand takes bacterium bag, and the right hand takes rope to beat a knot that can be undone by a pull from the collarium place of bacterium bag upper end, and the right hand must all the time by rope, successively bacterium bag is tied up on rope by knot that can be undone by a pull, until a fast knot beaten by last bacterium bag from the collarium place of bacterium bag upper end, the right hand unclamps rope, by bacterium bag deadweight nature by rope, ensure that each bacterium bag can not come off, and keep at least overhead 20 centimeters, last bacterium bag;
(8) syrinx reason is gone out: keep ear canopy temperature 25-30 DEG C, relative moisture 85-95%, under the condition of insulation, moisturizing, the temperature difference, scattered light, ventilation, ear bud starts to be formed, after ear bud occurs, based on moisturizing, suitable ventilation, when ear blastogenesis grows up to funnel-form or cup-shaped children ear, every day sprays water 2-3 time, based on ground or space water spray, water spray preferably nebulizes, and avoid and directly spray water to ear bag, growing up gradually with auricle suitably increases injection flow rate; At auricle Later growth, often to observe temperature of shed, temperature more than 30 DEG C, relative moisture higher than 95% time, answer stronger ventilation amount, reducing temperature and humidity; When miscellaneous bacteria occurs, do not use agricultural chemicals, spray bacteria agent carbendazim again after available limewash sterilization, and strengthen ventilation;
(9) pick and process: when auricle fully launch, edge starts to gather when starting to shrink, and generally needs 15-25 days from going out former base to gathering; The basal part of the ear must be adopted to the greatest extent when gathering, in order to avoid miscellaneous bacteria infects or insect pest causes rotten ear, affect the growth of next group auricularia auriculajudae; After auricle is gathered, stop water spray 3 days, allow mycelia restoration ecosystem, proceed out syrinx reason, generally can adopt ear 3-4 time continuously; After auricularia auriculajudae is gathered, remove ear base root composts or fertilisers of cultivating, dry.
Described collarium profile is T-shape, and does not use tampon to close sack, but collarium lid carrys out closed sack on collarium upper port cover.
The wide 7--8 rice of described ear canopy, long 30--35 rice, high 2.2-2.5 rice, ear canopy two is equipped with double swing door, and often string hangs 8-15 bacterium bag, can cultivate 6000-8000 bag, within 1 year, can cultivate 2 batches in canopy.
The advantage that the present invention has and effect:
1, the present invention adopts batch production Pig three-dimensional planting mode, and floor space is little, and temp. and humidity is easy to control, and ventilates, and might as well gather, without cultivation dead angle, not only reduce cost, save artificial, and output is high, quality is good, and Yellow-back fungus shape U.S. of production, color and luster is bright, and product are of fine quality.Also solve a difficult problem such as Yellow-back fungus children ear phase stream ear, green mold pollution etc., for Yellow-back fungus batch production, scale, industrialization cultivation provide a practicable method simultaneously.
2, in bacterium bag of the present invention, nutrient component fully can be absorbed by Yellow-back fungus mycelia, and planting material availability, up to 98%, goes out ear rate and becomes ear rate up to 98%, and rotten ear and green mold pollution loss rate reduce by more than 85% than traditional cultivation pattern.
3, the present invention has little collarium, not only good sealing effect at each bacterium bag sack cover, and inoculation is convenient, and a rope can be realized easily by little collarium and complete Pig operation, make loaded down with trivial details Pig, unload bag process and become simple and convenient, significantly improve the efficiency of Pig, shorten the time of Pig.
Accompanying drawing explanation
Fig. 1 is that the present invention inoculates front bacterium bag sealing schematic diagram, and Fig. 2 is that the present invention inoculates rear bacterium bag sealing schematic diagram, and Fig. 3 is ear booth structure schematic diagram of the present invention, and Fig. 4 is bacterium bag of the present invention hanging schematic diagram.
In figure, 1 is bacterium bag, and 2 is collarium, and 3 is loop-carrier, and 4 is rubber band, and 5 is tampon.
Embodiment
The cultivation method of a kind of Yellow-back fungus of 1,2,3,4 detailed description by reference to the accompanying drawings.
(1) planting material is prepared and poach by following weight ratio: cotton seed hulls 35%, maize cob meal 35%, weed tree sawdust 35%, bran chaff 15%, brown sugar 1.5%, calcium carbonate or gypsum 1.5%, superphosphate 1.5%; Water content 55%-60%, pH value is 7-7.5;
(2) pack: bacterium bag is polypropylene or polyethylene plastic bag, specification is diameter 17cm, height 33cm-35cm.The packed siccative 500-550 gram of each bacterium, and put collarium by after the bacterium bag upper end reducing installing planting material, then in collarium, insert loop-carrier, and make loop-carrier be positioned at bacterium bag central authorities, again bacterium bag sack is outwards routed up the outside being enclosed within collarium, and with rubber band, bacterium bag sack is tied tight on collarium;
(3) sterilizing: normal-pressure sterilization temperature 100 DEG C, continues 24-25 hour;
(4) inoculate: when the bacterium bag after sterilizing is cooled to below 25 DEG C, extract loop-carrier, make bacterium bag central authorities form a circular port post, then aseptically adopt inoculating gun to be injected from circular port post by Yellow-back fungus bacterial classification, and in collarium, fill in tampon close sack; Also can after inoculation completes on collarium upper port cover collarium lid carry out closed sack;
(5) bacteria: postvaccinal bacterium bag wall-is deposited in and sends out bacterium room, cultivate under 23 DEG C of-25 DEG C of constant temperature and send out bacterium, incubation time 40 ~ 48 days, makes mycelia cover with bacterium bag;
(6) designated port: after bacterium bag is immersed disinfecting solution of potassium permanganate process, covering with the bacterium bag surrounding of mycelia, drawing " V " shape mouth 10-12 with sterile blade, wall-stacks 5-7 days, and covers straw screen or mat, prepares Pig after knife-edge part mycelia is recovered;
(7) Pig: after bacterium bag is immersed disinfecting solution of potassium permanganate process, then carry out Pig at ear canopy, string spacing 10 ~ 15 centimeters during Pig, bag spacing 3 ~ 5 centimeters; Often string only needs a rope, rope upper end is fixed on ear ceiling portion, then left hand takes bacterium bag, and the right hand takes rope to beat a knot that can be undone by a pull from the collarium place of bacterium bag upper end, and the right hand must all the time by rope, successively bacterium bag is tied up on rope by knot that can be undone by a pull, until a fast knot beaten by last bacterium bag from the collarium place of bacterium bag upper end, the right hand unclamps rope, by bacterium bag deadweight nature by rope, ensure that each bacterium bag can not come off, and keep at least overhead 20 centimeters, last bacterium bag;
(8) syrinx reason is gone out: keep ear canopy temperature 25-30 DEG C, relative moisture 85-95%, under the condition of insulation, moisturizing, the temperature difference, scattered light, ventilation, ear bud starts to be formed, after ear bud occurs, based on moisturizing, suitable ventilation, when ear blastogenesis grows up to funnel-form or cup-shaped children ear, every day sprays water 2-3 time, based on ground or space water spray, water spray preferably nebulizes, and avoid and directly spray water to ear bag, growing up gradually with auricle suitably increases injection flow rate; At auricle Later growth, often to observe temperature of shed, temperature more than 30 DEG C, relative moisture higher than 95% time, answer stronger ventilation amount, reducing temperature and humidity; When miscellaneous bacteria occurs, do not use agricultural chemicals, spray bacteria agent carbendazim again after available limewash sterilization, and strengthen ventilation;
(9) pick and process: when auricle fully launch, edge starts to gather when starting to shrink, and generally needs 15-25 days from going out former base to gathering; The basal part of the ear must be adopted to the greatest extent when gathering, in order to avoid miscellaneous bacteria infects or insect pest causes rotten ear, affect the growth of next group auricularia auriculajudae; After auricle is gathered, stop water spray 3 days, allow mycelia restoration ecosystem, proceed out syrinx reason, generally can adopt ear 3-4 time continuously; After auricularia auriculajudae is gathered, remove ear base root composts or fertilisers of cultivating, dry.
Described collarium profile is T-shape, prevents rubber band from coming off on the one hand, blocks rope, prevent rope from coming off when being beneficial to the hanging of bacterium bag on the other hand.Described ear canopy is built by the curved steel tube of spacing 80 centimeters, wide 7--8 rice, long 30--35 rice, high 2.2-2.5 rice, ear canopy two is provided with double swing door, 18-20 string capable of hoisting on every root curved steel tube, often string hangs 8-15 bacterium bag, can cultivate 6000-8000 bag, within 1 year, can cultivate 2 batches in canopy.Described double swing door is made up of two fan doors, and a fan opens outward, a fan is open inward, during use, remains that Yishanmen is in closed condition, reduces the indoor-outdoor air convection current of opening the door and causing.
Often go here and there during Pig of the present invention and only need a rope, and the knot that can be undone by a pull of outstanding flagging mushroom bag product the ear phase can draw more tight, bacterium bag difficult drop-off, and untie after having gone out ear bottom an all bacterium bag of bacterium bag fast knot can all come off, namely shorten the duration, again reduce labour cost.Because rope is inside booth, not easily aging, can fold after being finished, the coming year directly can be used again, and a collection of rope can use 3-4, reduce further cost, also saves artificial.
Above-described embodiment, just preferred embodiment of the present invention, is not used for limiting the scope of the present invention, therefore all equivalent variations done with content described in the claims in the present invention, all should be included within the claims in the present invention scope.

Claims (3)

1. a cultivation method for Yellow-back fungus, is characterized in that comprising the steps:
(1) planting material is prepared and poach by following weight ratio: cotton seed hulls 30-40%, maize cob meal 30-40%, weed tree sawdust 30-40%, bran chaff 10-20%, brown sugar 1-2%, calcium carbonate or gypsum 1-2%, superphosphate 1-2%; Water content 55%-60%, pH value is 7-7.5;
(2) pack: the packed siccative 500-550 gram of each bacterium, and put collarium by after the bacterium bag upper end reducing installing planting material, then in collarium, loop-carrier is inserted, and make loop-carrier be positioned at bacterium bag central authorities, again bacterium bag sack is outwards routed up the outside being enclosed within collarium, and with rubber band, bacterium bag sack is tied tight on collarium;
(3) sterilizing: normal-pressure sterilization temperature 100 DEG C, continues 24-25 hour;
(4) inoculate: when the bacterium bag after sterilizing is cooled to below 25 DEG C, extract loop-carrier, make bacterium bag central authorities form a circular port post, then aseptically adopt inoculating gun to be injected from circular port post by Yellow-back fungus bacterial classification, and in collarium, fill in tampon close sack;
(5) bacteria: postvaccinal bacterium bag wall-is deposited in and sends out bacterium room, cultivate under 23 DEG C of-25 DEG C of constant temperature and send out bacterium, incubation time 40 ~ 48 days, makes mycelia cover with bacterium bag;
(6) designated port: after bacterium bag is immersed disinfecting solution of potassium permanganate process, covering with the bacterium bag surrounding of mycelia, drawing " V " shape mouth 10-12 with sterile blade, wall-stacks 5-7 days, and covers straw screen or mat, prepares Pig after knife-edge part mycelia is recovered;
(7) Pig: carry out Pig at ear canopy, string spacing 10 ~ 15 centimeters during Pig, bag spacing 3 ~ 5 centimeters; Often string only needs a rope, rope upper end is fixed on ear ceiling portion, then left hand takes bacterium bag, and the right hand takes rope to beat a knot that can be undone by a pull from the collarium place of bacterium bag upper end, and the right hand must all the time by rope, successively bacterium bag is tied up on rope by knot that can be undone by a pull, until a fast knot beaten by last bacterium bag from the collarium place of bacterium bag upper end, the right hand unclamps rope, by bacterium bag deadweight nature by rope, ensure that each bacterium bag can not come off, and keep at least overhead 20 centimeters, last bacterium bag;
(8) syrinx reason is gone out: keep ear canopy temperature 25-30 DEG C, relative moisture 85-95%, under the condition of insulation, moisturizing, the temperature difference, scattered light, ventilation, ear bud starts to be formed, after ear bud occurs, based on moisturizing, suitable ventilation, when ear blastogenesis grows up to funnel-form or cup-shaped children ear, every day sprays water 2-3 time, based on ground or space water spray, water spray preferably nebulizes, and avoid and directly spray water to ear bag, growing up gradually with auricle suitably increases injection flow rate; At auricle Later growth, often to observe temperature of shed, temperature more than 30 DEG C, relative moisture higher than 95% time, answer stronger ventilation amount, reducing temperature and humidity; When miscellaneous bacteria occurs, do not use agricultural chemicals, spray bacteria agent carbendazim again after available limewash sterilization, and strengthen ventilation;
(9) pick and process: when auricle fully launch, edge starts to gather when starting to shrink, and generally needs 15-25 days from going out former base to gathering; The basal part of the ear must be adopted to the greatest extent when gathering, in order to avoid miscellaneous bacteria infects or insect pest causes rotten ear, affect the growth of next group auricularia auriculajudae; After auricle is gathered, stop water spray 3 days, allow mycelia restoration ecosystem, proceed out syrinx reason, generally can adopt ear 3-4 time continuously; After auricularia auriculajudae is gathered, remove ear base root composts or fertilisers of cultivating, dry.
2. the cultivation method of Yellow-back fungus according to claim 1, is characterized in that: described collarium profile is T-shape, and does not use tampon to close sack, but collarium lid carrys out closed sack on collarium upper port cover.
3. the cultivation method of Yellow-back fungus according to claim 1 and 2, is characterized in that: the wide 7--8 rice of described ear canopy, long 30--35 rice, high 2.2-2.5 rice, ear canopy two is equipped with double swing door, and often string hangs 8-15 bacterium bag, 6000-8000 bag can be cultivated in canopy, within 1 year, 2 batches can be cultivated.
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Cited By (8)

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Publication number Priority date Publication date Assignee Title
CN105130686A (en) * 2015-10-09 2015-12-09 四川省农业科学院植物保护研究所 Culture medium and preparation method thereof and application of culture medium in auriailaria polytricha cultivation
CN105557309A (en) * 2016-01-04 2016-05-11 山东省科创食用菌产业技术研究院 Greenhouse sack hanging pinhole single-piece culture method for auricularia fuscosuccinea
CN106489541A (en) * 2016-12-20 2017-03-15 福建农林大学 A kind of Ganoderma fruiting method
CN107278624A (en) * 2017-08-15 2017-10-24 贵州天圣绿野生物科技有限公司 A kind of propagation method of agaric
CN109757308A (en) * 2019-03-28 2019-05-17 什邡昊阳农业发展有限公司 A kind of Yellow-back fungus capsule inoculation method
CN109845575A (en) * 2019-02-01 2019-06-07 云南菌视界生物科技有限公司 A method of shortening the hickory chick Spawn incubation period
TWI688334B (en) * 2019-03-15 2020-03-21 林俊義 Fungus cultivation method
CN117136788A (en) * 2023-10-11 2023-12-01 湖北飘扬食品科技有限公司 Morchella strain preparation method

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105130686A (en) * 2015-10-09 2015-12-09 四川省农业科学院植物保护研究所 Culture medium and preparation method thereof and application of culture medium in auriailaria polytricha cultivation
CN105130686B (en) * 2015-10-09 2018-12-14 四川省农业科学院植物保护研究所 The application of a kind of compost and preparation method thereof and compost in Yellow-back fungus cultivation
CN105557309A (en) * 2016-01-04 2016-05-11 山东省科创食用菌产业技术研究院 Greenhouse sack hanging pinhole single-piece culture method for auricularia fuscosuccinea
CN105557309B (en) * 2016-01-04 2019-04-26 山东省科创食用菌产业技术研究院 A kind of jade agaric greenhouse hanging bag aperture monolithic cultural method
CN106489541A (en) * 2016-12-20 2017-03-15 福建农林大学 A kind of Ganoderma fruiting method
CN107278624A (en) * 2017-08-15 2017-10-24 贵州天圣绿野生物科技有限公司 A kind of propagation method of agaric
CN109845575A (en) * 2019-02-01 2019-06-07 云南菌视界生物科技有限公司 A method of shortening the hickory chick Spawn incubation period
CN109845575B (en) * 2019-02-01 2021-10-01 云南菌视界生物科技有限公司 Method for shortening culture period of morchella strain
TWI688334B (en) * 2019-03-15 2020-03-21 林俊義 Fungus cultivation method
CN109757308A (en) * 2019-03-28 2019-05-17 什邡昊阳农业发展有限公司 A kind of Yellow-back fungus capsule inoculation method
CN117136788A (en) * 2023-10-11 2023-12-01 湖北飘扬食品科技有限公司 Morchella strain preparation method

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