CN107231941A - A kind of Hericium erinaceus culture method - Google Patents
A kind of Hericium erinaceus culture method Download PDFInfo
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- CN107231941A CN107231941A CN201710415052.9A CN201710415052A CN107231941A CN 107231941 A CN107231941 A CN 107231941A CN 201710415052 A CN201710415052 A CN 201710415052A CN 107231941 A CN107231941 A CN 107231941A
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Abstract
The invention discloses a kind of Hericium erinaceus culture method, step：(1) production of hybrid seeds culture material formula；(2) bacterium nail kind inoculated and cultured；(3) making and sterilizing of Hericium erinaceus culture bacterium bag；(4) inoculation of hericium erinaceus rod；(5) culture of hericium mycelium；(6) management of producing mushroom of hericium erinaceus rod；(7) environmental condition regulates and controls during fruiting；(8) harvest：Bacteria thorn length 1.0cm, spore is not distributed also.The present invention is shortened the cell age of hedgehog fungus bacterial, is improved strain quality using the method for plastics bacterium nail kind cultivation Hericium erinaceus；The planting technique of plastic bag cultivation Hericium erinaceus is innovated, accelerates inoculation speed, saves artificial, shortens Hericium erinaceus stem, improve product commodity.
Description
Technical field
The present invention relates to planting edible mushroom technical field, more particularly to a kind of Hericium erinaceus culture method.
Background technology
Hericium erinaceus, also known as hedgehog bacterium, cauliflower bacterium, are food medicine dual-purpose fungies well-known at home and abroad, because it is of high nutritive value, element
There is the good reputation of " mountain delicacy ".According to《Compendium of Materia Medica》Record, Hericium erinaceus is mild-natured, sweet, have relieving the five internal organs, an aid digestion function.Cure in the recent period
Learn research and also demonstrate that Hericium erinaceus contains the various active materials such as polysaccharide, oligosaccharides, polypeptide, sterol, mushroom class, phenols, adenosine, has
The health care functions such as anti-oxidant, antitumor, hypoglycemic, norcholesterol, nourishing, liver protection, stomach invigorating, enhancing immunity of organisms.With
The food of Hericium erinaceus, medical value to be gradually recognized, the market development prospect of hedgehog hydnum is more and more wide, but planted due to various
Technical factor is trained, in recent years, Hericium erinaceus there are no fairly large production.
Hericium mycelium is thin and delicate, and anti-miscellaneous bacteria, resistance are poor, and strain quality and inoculation operation are to the success of hericium erinaceus rod
Rate, the commodity of Hericium erinaceus influence very big.The hedgehog fungus bacterial that conventional method makes, is extruded during inoculation by artificial and bacterium bag, is held
Mycelia damage is easily caused, influence mycelia recovers, and reduces strain survival rate.Hericium mycelium growth is slow, but the cell age time is short, generally
Mycelia do not cover with even just long 20d or so will fruiting, therefore, the strain that conventional method makes occurs that front end strain aging goes out
Mushroom, and the jejune phenomenon of bottom strain.Because hericium mycelium growth is slow, the required multiple spot inoculation of bacterium rod.Traditional cultivation hedgehog hydnum
Mushroom is generally inoculated with or punched inoculation using two., be in sack two ends set without cotton lid neck ring or two ends jag when two is inoculated with；
Punching inoculation, generally requires 3~4 people and operates (punching, inoculation, rubberized fabric sealing, pendulum bag) together, sterilize and operate during inoculation
Required tight, both approaches are inoculated with troublesome poeration, and speed is slow, needs labour more.The monkey produced using traditional producing method for seed
, during production bacterium rod, there is pollution rate height, labor cost is high in head mushroom and vaccination ways, add fruiting environmental condition and require high, make
It is low into biological transformation ratio, the economic benefit of production is reduced, the modelling production of Hericium erinaceus is had a strong impact on.
The information for being disclosed in the background section is merely intended to understanding of the increase to the general background of the present invention, without answering
When the prior art for being considered as recognizing or implying the information structure in any form well known to persons skilled in the art.
The content of the invention
The present invention is directed to the problems of the prior art, invents a kind of Hericium erinaceus culture method, it is intended to which obtaining one kind can carry
High strain quality, reduction productivity needs, simplifies process, the Hericium erinaceus culture mode for reducing cost, improving product commodity.
To achieve the above object, the technical scheme that the present invention is provided is as follows：
A kind of Hericium erinaceus culture method, includes following operating procedure：
(1) production of hybrid seeds culture material formula：Production of hybrid seeds raw material is taken, each raw material is well mixed, and adjusts mixed raw material moisture
For 60~65%, pH=5.5~6.0, continuously connected fastener is sufficiently mixed with the raw material prepared, and raw material then is inserted into plastics by jamming on
In nail, unnecessary raw material is sifted out, packed, sterilizing produces continuously connected fastener culture medium；
(2) bacterium nail kind inoculated and cultured：Continuously connected fastener culture medium after sterilizing is cooled to less than 28 DEG C in transfer room to be inoculated with,
Inoculum concentration is culture, when longer mycelia to be generated has thorough grasp the raw material in continuously connected fastener completely untill strain can be paved with sack charge level
Produce bacterium nail kind；
(3) making and sterilizing of Hericium erinaceus culture bacterium bag：Bacterium bag raw material is taken, hedgehog hydnum is produced according to conventional Hericium erinaceus culture
Mushroom bag, compost dress polybag produces bacterium bag, and pack elasticity is suitable, and bacterium bag will sterilize in time；
(4) inoculation of hericium erinaceus rod：Bacterium bag after sterilizing is cooled to less than 28 DEG C and can be inoculated with, and aseptically grasps
Make, during inoculation, bacterium bag traverse is inoculated with, during inoculation, firmly press in bacterium nail kind obtained by step (2) in bacterium bag, until bacterium
The head of a nail of nail kind is close to a bag wall, produces bacterium rod；
(5) culture of hericium mycelium：After finished, bacterium rod is put or is put on the spot in other culturing room and cultivated；
(6) management of producing mushroom of hericium erinaceus rod：There is Hericium erinaceus at small part bacterium rod inoculation in culture 25 days or so
During former base, management of producing mushroom is carried out, you can move out of bacterium rod to mushroom shed (room) and carry out fruiting；
(7) environmental condition regulates and controls during fruiting：
Humidity regulation, indoor air humidity is maintained at 85~90%, it is impossible to be less than less than 70% or higher than 95%, such as humidity
70% sporophore growth is slow, yellowish, drying shrinkage；Humidity is higher than 95% for a long time, easily grows miscellaneous bacteria and disease, causes mushroom body to be sent out
Mould to rot, water spray must combine ventilation, make air fresh, fructification grows vigorously；
During temperature adjusting, fruiting, mushroom room temperature should be maintained at 15~22 DEG C；
(8) harvest：The optimal harvest time of Hericium erinaceus is the ripe mid-term of fructification, bacteria thorn length 1.0cm or so, and spore is also not
Distribute, now quality is solid, bitter taste is few, quality is high, collecting method：Fructification root is held, first twists and gently extracts again, also may be used
Use scraper tapping.
Preferably, continuously connected fastener is soaked 0.5~1 hour with clear water before the continuously connected fastener production of hybrid seeds described in step (1)；Described system
It is weed tree sawdust 76.9%, wheat bran 20%, potassium dihydrogen phosphate 0.1%, yellow sand sugar 1%, peroxophosphoric acid that raw material, which is planted, according to mass percent
Calcium 1%, precipitated calcium carbonate 1%.
Preferably, packed with 15cm × 26~28cm × 0.05cm strain bags in step (1), keeping temperature is 126 DEG C,
Pressure is 0.14Mpa autoclavings 2.5 hours.
Preferably, 25~28 DEG C of Spawn incubation temperature in step (2), air humidity 65%, room air keeps fresh,
Strain is cultivated by 30d, and longer mycelia to be generated produces bacterium nail kind when having thorough grasp the raw material in continuously connected fastener completely.
Preferably, the bacterium bag raw material described in step (3) includes following components by mass percentage：Biological material
50.9%th, cotton seed hull 27%, wheat bran 17%, corn flour 3%, gypsum 1%, calcium superphosphate 1% and potassium dihydrogen phosphate 0.1%, it is raw
Hericium erinaceus bacterium bag is produced, gained bacterium bag water content 65%, pH are 6.0 or so；Described specification of plastic bag width x length × thickness is 17cm
× 33~38cm × 0.04~0.05cm or 13 × 50cm × 0.04~0.05cm normal pressure polyethylene or high-pressure polypropylene bag.
Preferably, described biological material is one kind in ramulus mori, bagasse or weed tree sawdust.
Preferably, the sterilizing described in step (3) is normal-pressure sterilization, when temperature is raised to 100 DEG C after keep 10~12 small
When or under 126 DEG C, 0.14Mpa autoclaving keep 2.5 hours.
Preferably, inoculum density is that every 7~8cm connects 1 bacterium nail in step (4).
Preferably, culturing room's relative humidity described in step (5) is below 65%, well-ventilated, and indoor temperature is kept
It is 25~28 DEG C, dark or have weaker scattered light；Cultivate and strain sprouting situation is checked after 4~6d, mending in time for not sprouting connects
Kind, pollution is checked after inoculation 10d, pollution cleaning in time is found.
Preferably, the management of producing mushroom of bacterium rod described in step (6) is the bacterium nail for pulling out inoculation mouth, 25 DEG C of left sides of keeping temperature
The right side, allows after the mycelia of inoculation mouth recovers 3~5 days, then moves out of mushroom shed (room) fruiting to.
Preferably, the environmental condition regulation and control described in step (7) also include：
Fructification is especially sensitive to carbon dioxide during ventilation regulation and control, fruiting, now requires mushroom house well-ventilated, keeps empty
Gas is fresh, but cold wind can not be allowed to be directly blown onto in fructification；
Illumination regulates and controls, and mushroom house seven second of holding, three points of positive scattered lights were beneficial to the development of hericium erinaceus fruiting body.
Compared with prior art, the present invention has the advantages that：
The present invention is shortened the cell age of hedgehog fungus bacterial, is improved strain matter using the method for plastics bacterium nail kind cultivation Hericium erinaceus
Amount；The planting technique of plastic bag cultivation Hericium erinaceus is innovated, accelerates inoculation speed, saves artificial, shortens Hericium erinaceus stem, improve product commodity
Property.
Embodiment
It is described in detail with reference to embodiment, it is to be understood that protection scope of the present invention is not by specific
The limitation of embodiment.
Embodiment 1
A kind of Hericium erinaceus culture method, includes following operating procedure：
(1) production of hybrid seeds culture material formula：Before the production of hybrid seeds continuously connected fastener is soaked with clear water 0.5 hour, it is standby；Taken according to mass percent
Following raw material, weed tree sawdust 76.9%, wheat bran 20%, potassium dihydrogen phosphate 0.1%, yellow sand sugar 1%, calcium superphosphate 1%, lightweight carbonic acid
Calcium 1%；Each raw material is well mixed, it is 60%, pH=5.5 then to adjust mixed raw material moisture, will be standby after immersion
Continuously connected fastener is sufficiently mixed with the raw material prepared, and raw material is inserted into bacterium nail gap by jamming on, and then sifts out unnecessary raw material,
Packed with 15cm × 26cm × 0.05cm strain bags, keeping temperature is 126 DEG C, pressure is 0.14Mpa autoclavings 2.5 hours,
Produce continuously connected fastener culture medium；
(2) bacterium nail kind inoculated and cultured：Continuously connected fastener culture medium after sterilizing is cooled to less than 28 DEG C in transfer room to be inoculated with,
Inoculum concentration is Spawn incubation condition untill strain can be paved with sack charge level：25~28 DEG C of temperature, air humidity 65%, Interior Space
Gas keeps fresh, and strain was cultivated by 30 days or so, and mycelia has thorough grasp the culture for being during the compost in continuously connected fastener gap completely
Good bacterium nail kind；
(3) making and sterilizing of Hericium erinaceus culture bacterium bag：Include following components by mass percentage：Ramulus mori 50.9%, cotton
Sub- shell 27%, wheat bran 17%, corn flour 3%, gypsum 1%, calcium superphosphate 1% and potassium dihydrogen phosphate 0.1%, according to conventional monkey
Head mushroom cultivation method production Hericium erinaceus bacterium bag, bacterium bag water content 65%, pH are 6.0 or so, and specification of plastic bag width x length × thickness is
17cm × 33cm × 0.04cm normal pressure Polythene Bag, compost dress polybag elasticity is suitable, produces bacterium bag, and bacterium bag is timely
Sterilizing, normal-pressure sterilization, when temperature is raised to 100 DEG C after kept for 10 hours；
(4) inoculation of hericium erinaceus rod：Bacterium bag after sterilizing is cooled to less than 28 DEG C and can be inoculated with, and aseptically grasps
Make, during inoculation, bacterium bag traverse is inoculated with, every 7~8cm connects 1 bacterium nail, during inoculation, firmly presses in bacterium nail in bacterium bag, directly
The head of a nail planted is followed closely to bacterium and is close to a bag wall, bacterium rod is produced；
(5) culture of hericium mycelium：After finished, the culture of other culturing room, culturing room are put or be put on the spot to bacterium rod
It is required that being air-dried, relative humidity is below 65%, well-ventilated, and indoor temperature is kept for 25~28 DEG C, dark or have weaker dissipate
Strain sprouting situation is checked after penetrating light, 4~6d of inoculation, that does not sprout will mend inspection pollution after inoculation, inoculation 10d in time, find
Pollution cleaning in time；
(6) management of producing mushroom of hericium erinaceus rod：There is monkey at small part bacterium rod inoculation in cultural hypha 25 days or so
During head mushroom former base, management of producing mushroom is carried out, that is, pulls out the bacterium nail of inoculation mouth, 25 DEG C or so of keeping temperature makes the mycelia of inoculation mouth extensive
After multiple 3~5 days, you can move out of bacterium rod to mushroom shed (room) and carry out fruiting；
(7) environmental condition regulates and controls during fruiting：
Humidity regulation, indoor air humidity is maintained at 85~90%, it is impossible to be less than less than 70% or higher than 95%, such as humidity
70% sporophore growth is slow, yellowish, drying shrinkage；Humidity is higher than 95% for a long time, easily grows miscellaneous bacteria and disease, causes mushroom body to be sent out
Mould to rot, water spray must combine ventilation, make air fresh, fructification grows vigorously；
During temperature adjusting, fruiting, mushroom room temperature should be maintained at 15~22 DEG C；
Fructification is especially sensitive to carbon dioxide during ventilation regulation and control, fruiting, now requires mushroom house well-ventilated, keeps empty
Gas is fresh, but cold wind can not be allowed to be directly blown onto in fructification；
Illumination regulates and controls, and mushroom house seven second of holding, three points of positive scattered lights were beneficial to the development of hericium erinaceus fruiting body；
(8) harvest：The optimal harvest time of Hericium erinaceus is the ripe mid-term of fructification, bacteria thorn length 1.0cm or so, and spore is also not
Distribute, now quality is solid, bitter taste is few, quality is high, collecting method：Fructification root is held, first twists and gently extracts again, also may be used
Use scraper tapping.
Embodiment 2
A kind of Hericium erinaceus culture method, includes following operating procedure：
(1) production of hybrid seeds culture material formula：Before the production of hybrid seeds continuously connected fastener is soaked with clear water 1 hour, it is standby；According to mass percent take with
Lower raw material, weed tree sawdust 76.9%, wheat bran 20%, potassium dihydrogen phosphate 0.1%, yellow sand sugar 1%, calcium superphosphate 1%, precipitated calcium carbonate
1%；Each raw material is well mixed, it is 65%, pH=6.0 then to adjust mixed raw material moisture, by modeling standby after immersion
Material nail is sufficiently mixed with the raw material prepared, and raw material is inserted into bacterium nail gap by jamming on, and then sifts out unnecessary raw material, is used
15cm × 28cm × 0.05cm strain bags pack, keeping temperature is 126 DEG C, pressure is 0.14Mpa autoclavings 2.5 hours, i.e.,
Obtain continuously connected fastener culture medium；
(2) bacterium nail kind inoculated and cultured：Continuously connected fastener culture medium after sterilizing is cooled to less than 28 DEG C in transfer room to be inoculated with,
Inoculum concentration is Spawn incubation condition untill strain can be paved with sack charge level：25~28 DEG C of temperature, air humidity 65%, Interior Space
Gas keeps fresh, and strain was cultivated by 30 days or so, and mycelia has thorough grasp the culture for being during the compost in continuously connected fastener gap completely
Good bacterium nail kind；
(3) making and sterilizing of Hericium erinaceus culture bacterium bag：Include following components by mass percentage：Bagasse 50.9%,
Cotton seed hull 27%, wheat bran 17%, corn flour 3%, gypsum 1%, calcium superphosphate 1% and potassium dihydrogen phosphate 0.1%, according to routine
Hericium erinaceus plants production Hericium erinaceus bacterium bag, bacterium bag water content 65%, pH 6.0 or so, specification of plastic bag width x length × thickness is 13 ×
50cm × 0.05cm high-pressure polypropylene bag, compost dress polybag elasticity is suitable, produces bacterium bag, bacterium bag will sterilize in time,
Autoclaving 2.5 hours under 126 DEG C, 0.14Mpa；
(4) inoculation of hericium erinaceus rod：Bacterium bag after sterilizing is cooled to less than 28 DEG C and can be inoculated with, and aseptically grasps
Make, during inoculation, bacterium bag traverse is inoculated with, every 7~8cm connects 1 bacterium nail, during inoculation, firmly presses in bacterium nail in bacterium bag, directly
The head of a nail planted is followed closely to bacterium and is close to a bag wall, bacterium rod is produced；
(5) culture of hericium mycelium：After finished, the culture of other culturing room, culturing room are put or be put on the spot to bacterium rod
It is required that being air-dried, relative humidity is below 65%, well-ventilated, and indoor temperature is kept for 25~28 DEG C, dark or have weaker dissipate
Strain sprouting situation is checked after penetrating light, 4~6d of inoculation, that does not sprout will mend inspection pollution after inoculation, inoculation 10d in time, find
Pollution cleaning in time；
(6) management of producing mushroom of hericium erinaceus rod：There is monkey at small part bacterium rod inoculation in cultural hypha 25 days or so
During head mushroom former base, management of producing mushroom is carried out, that is, pulls out the bacterium nail of inoculation mouth, 25 DEG C or so of keeping temperature makes the mycelia of inoculation mouth extensive
After multiple 3~5 days, you can move out of bacterium rod to mushroom shed (room) and carry out fruiting；
(7) environmental condition regulates and controls during fruiting：
Humidity regulation, indoor air humidity is maintained at 85~90%, it is impossible to be less than less than 70% or higher than 95%, such as humidity
70% sporophore growth is slow, yellowish, drying shrinkage；Humidity is higher than 95% for a long time, easily grows miscellaneous bacteria and disease, causes mushroom body to be sent out
Mould to rot, water spray must combine ventilation, make air fresh, fructification grows vigorously；
During temperature adjusting, fruiting, mushroom room temperature should be maintained at 15~22 DEG C；
Fructification is especially sensitive to carbon dioxide during ventilation regulation and control, fruiting, now requires mushroom house well-ventilated, keeps empty
Gas is fresh, but cold wind can not be allowed to be directly blown onto in fructification；
Illumination regulates and controls, and mushroom house seven second of holding, three points of positive scattered lights were beneficial to the development of hericium erinaceus fruiting body；
(8) harvest：The optimal harvest time of Hericium erinaceus is the ripe mid-term of fructification, bacteria thorn length 1.0cm or so, and spore is also not
Distribute, now quality is solid, bitter taste is few, quality is high, collecting method：Fructification root is held, first twists and gently extracts again, also may be used
Use scraper tapping.
Embodiment 3
A kind of Hericium erinaceus culture method, includes following operating procedure：
(1) production of hybrid seeds culture material formula：Before the production of hybrid seeds continuously connected fastener is soaked with clear water 0.7 hour, it is standby；Taken according to mass percent
Following raw material, weed tree sawdust 76.9%, wheat bran 20%, potassium dihydrogen phosphate 0.1%, yellow sand sugar 1%, calcium superphosphate 1%, lightweight carbonic acid
Calcium 1%；Each raw material is well mixed, it is 63%, pH=5.7 then to adjust mixed raw material moisture, will be standby after immersion
Continuously connected fastener is sufficiently mixed with the raw material prepared, and raw material is inserted into bacterium nail gap by jamming on, and then sifts out unnecessary raw material,
Packed with 15cm × 27cm × 0.05cm strain bags, keeping temperature is 126 DEG C, pressure is 0.14Mpa autoclavings 2.5 hours,
Produce continuously connected fastener culture medium；
(2) bacterium nail kind inoculated and cultured：Continuously connected fastener culture medium after sterilizing is cooled to less than 28 DEG C in transfer room to be inoculated with,
Inoculum concentration is Spawn incubation condition untill strain can be paved with sack charge level：25~28 DEG C of temperature, air humidity 65%, Interior Space
Gas keeps fresh, and strain was cultivated by 30 days or so, and mycelia has thorough grasp the culture for being during the compost in continuously connected fastener gap completely
Good bacterium nail kind；
(3) making and sterilizing of Hericium erinaceus culture bacterium bag：Include following components by mass percentage：Weed tree sawdust 50.9%,
Cotton seed hull 27%, wheat bran 17%, corn flour 3%, gypsum 1%, calcium superphosphate 1% and potassium dihydrogen phosphate 0.1%, according to routine
Hericium erinaceus plants production Hericium erinaceus bacterium bag, and bacterium bag water content 65%, pH are 6.0 or so, and specification of plastic bag width x length × thickness is 17cm
× 38cm × 0.05cm normal pressure Polythene Bag, compost dress polybag elasticity is suitable, produces bacterium bag, bacterium bag will go out in time
Bacterium, autoclaving 2.5 hours under 126 DEG C, 0.14Mpa；
(4) inoculation of hericium erinaceus rod：Bacterium bag after sterilizing is cooled to less than 28 DEG C and can be inoculated with, and aseptically grasps
Make, during inoculation, bacterium bag traverse is inoculated with, every 7~8cm connects 1 bacterium nail, during inoculation, firmly presses in bacterium nail in bacterium bag, directly
The head of a nail planted is followed closely to bacterium and is close to a bag wall, bacterium rod is produced；
(5) culture of hericium mycelium：After finished, the culture of other culturing room, culturing room are put or be put on the spot to bacterium rod
It is required that being air-dried, relative humidity is below 65%, well-ventilated, and indoor temperature is kept for 25~28 DEG C, dark or have weaker dissipate
Strain sprouting situation is checked after penetrating light, 4~6d of inoculation, that does not sprout will mend inspection pollution after inoculation, inoculation 10d in time, find
Pollution cleaning in time；
(6) management of producing mushroom of hericium erinaceus rod：There is monkey at small part bacterium rod inoculation in cultural hypha 25 days or so
During head mushroom former base, management of producing mushroom is carried out, that is, pulls out the bacterium nail of inoculation mouth, 25 DEG C or so of keeping temperature makes the mycelia of inoculation mouth extensive
After multiple 3~5 days, you can move out of bacterium rod to mushroom shed (room) and carry out fruiting；
(7) environmental condition regulates and controls during fruiting：
Humidity regulation, indoor air humidity is maintained at 85~90%, it is impossible to be less than less than 70% or higher than 95%, such as humidity
70% sporophore growth is slow, yellowish, drying shrinkage；Humidity is higher than 95% for a long time, easily grows miscellaneous bacteria and disease, causes mushroom body to be sent out
Mould to rot, water spray must combine ventilation, make air fresh, fructification grows vigorously；
During temperature adjusting, fruiting, mushroom room temperature should be maintained at 15~22 DEG C；
Fructification is especially sensitive to carbon dioxide during ventilation regulation and control, fruiting, now requires mushroom house well-ventilated, keeps empty
Gas is fresh, but cold wind can not be allowed to be directly blown onto in fructification；
Illumination regulates and controls, and mushroom house seven second of holding, three points of positive scattered lights were beneficial to the development of hericium erinaceus fruiting body；
(8) harvest：The optimal harvest time of Hericium erinaceus is the ripe mid-term of fructification, bacteria thorn length 1.0cm or so, and spore is also not
Distribute, now quality is solid, bitter taste is few, quality is high, collecting method：Fructification root is held, first twists and gently extracts again, also may be used
Use scraper tapping.
Comparative example 1
Step (2) vaccination ways change punching inoculation into：Punched, strain is tapped into hole, Ran Houyong on bacterium rod with wooden stick
Adhesive plaster is sealed；Other operations are same as Example 1.
Comparative example 2
Step (2) vaccination ways change the inoculation of bacterium bag two into：Polybag both ends open is inoculated with, and two ends set is without cotton lid after inoculation
Neck ring, two fruiting during fruiting；Other operations are same as Example 1.
According to the data for needing to detect and observing in table 1, embodiment 1, comparative example 1, comparative example 2 are united
Meter, the data obtained is as shown in table 1：
Table 1
Growth speed of the present invention is fast：There is a fixed gap between bacterium nail, good permeability, mycelial growth is fast, than conventional bacterium
Plant and shift to an earlier date 15d or so purseful；Bacterium rod inoculation survival rate is high：Bacterium nail kind is a complete fungus block, and mycelia is injury-free, it is to avoid
As traditional bacterial classification breaks into pieces with one's fingers in inoculation and causes mycelia to damage, the time of mycelia healing growth is reduced, mycelia restoration ecosystem is fast, can be
Material feeding in short period, survival rate is high；Save artificial：Inoculation is easy to operate, quick, is conventional two inoculation or punching inoculation
Artificial 1/3 needed for method；Bacterium rod high yield rate：It is inoculated with speed fast, inoculation mouth exposure is short in the outer time, reduces pollution rate；Monkey
Head mushroom commodity is high：The fruiting at bacterium nail vaccination, Hericium erinaceus mushroom shape rounding, high yield rate, stem is short and small, and commodity is good；Section
About cost of material：Continuously connected fastener after fruiting can be recycled.
It is foregoing to the present invention specific illustrative embodiment description be in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can be much changed
And change.The purpose of selecting and describing the exemplary embodiment is that explaining that the certain principles and its reality of the present invention should
With so that those skilled in the art can realize and using the present invention a variety of exemplaries and
A variety of selections and change.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (10)
1. a kind of Hericium erinaceus culture method, it is characterised in that include following operating procedure：
(1) production of hybrid seeds culture material formula：Production of hybrid seeds raw material is taken, each raw material is well mixed, and it is 60 to adjust mixed raw material moisture
~65%, pH=5.5~6.0, continuously connected fastener mixes with the raw material prepared, is then inserted raw material in continuously connected fastener by jamming on, dress
Bag, sterilizing, produces continuously connected fastener culture medium；
(2) bacterium nail kind inoculated and cultured：Continuously connected fastener culture medium after sterilizing is cooled to less than 28 DEG C and can be inoculated with, and inoculum concentration is strain
Untill sack charge level can be paved with, culture, longer mycelia to be generated produces bacterium nail kind when having thorough grasp the raw material in continuously connected fastener completely；
(3) making and sterilizing of Hericium erinaceus culture bacterium bag：Bacterium bag raw material is taken, Hericium erinaceus bacterium bag is produced, compost fills polybag, i.e.,
Obtain bacterium bag, sterilizing；
(4) inoculation of hericium erinaceus rod：Bacterium bag after sterilizing is cooled to less than 28 DEG C and can be inoculated with, and bacterium bag traverse is connect
Kind, during inoculation, firmly bacterium nail kind obtained by step (2) is pressed in bacterium bag, bacterium rod is produced；
(5) culture of hericium mycelium：After finished, bacterium rod is cultivated in culturing room；
(6) management of producing mushroom of hericium erinaceus rod：When cultivating 25 days or Hericium erinaceus former base occur at small part bacterium rod inoculation, enter
Row management of producing mushroom, you can move out of bacterium rod to mushroom shed and carry out fruiting；
(7) environmental condition regulates and controls during fruiting：Humidity regulation, indoor air humidity is maintained at 85~90%, and water spray must be combined
Ventilation, makes air fresh, fructification grows vigorously；
During temperature adjusting, fruiting, mushroom room temperature should be maintained at 15~22 DEG C；
(8) harvest：Bacteria thorn length 1.0cm, spore is not distributed also.
2. Hericium erinaceus culture method according to claim 1, it is characterised in that：Before the continuously connected fastener production of hybrid seeds described in step (1)
Continuously connected fastener is soaked with clear water 0.5~1 hour；Described production of hybrid seeds raw material is weed tree sawdust 76.9%, wheat bran according to mass percent
20%th, potassium dihydrogen phosphate 0.1%, yellow sand sugar 1%, calcium superphosphate 1%, precipitated calcium carbonate 1%.
3. Hericium erinaceus culture method according to claim 1, it is characterised in that：15cm × 26~28cm is used in step (1)
× 0.05cm strain bags are packed, and keeping temperature is 126 DEG C, pressure is 0.14Mpa autoclavings 2.5 hours.
4. Hericium erinaceus culture method according to claim 1, it is characterised in that：Spawn incubation temperature 25 in step (2)~
28 DEG C, air humidity 65%, strain is cultivated by 30d, when longer mycelia to be generated has thorough grasp the raw material in continuously connected fastener completely i.e.
Obtain bacterium nail kind.
5. Hericium erinaceus culture method according to claim 1, it is characterised in that：Bacterium bag raw material described in step (3) is pressed
Mass percent includes following components：Biological material 50.9%, cotton seed hull 27%, wheat bran 17%, corn flour 3%, gypsum
1%th, calcium superphosphate 1% and potassium dihydrogen phosphate 0.1%, gained bacterium bag water content 65%, pH are 6.0；Wherein, described biomass
Material is one kind in ramulus mori, bagasse or weed tree sawdust；Described specification of plastic bag width x length × thickness is 17cm × 33~38cm
× 0.04~0.05cm or 13 × 50cm × 0.04~0.05cm normal pressure polyethylene or high-pressure polypropylene bag.
6. Hericium erinaceus culture method according to claim 1, it is characterised in that：Sterilizing described in step (3) is normal pressure
Sterilizing, when temperature is raised to 100 DEG C after kept for 10~12 hours or autoclaving is kept for 2.5 hours under 126 DEG C, 0.14Mpa.
7. Hericium erinaceus culture method according to claim 1, it is characterised in that：In step (4) inoculum density be every 7~
8cm connects 1 bacterium nail.
8. Hericium erinaceus culture method according to claim 1, it is characterised in that：Culturing room described in step (5) is relative
Below humidity 65%, well-ventilated, indoor temperature is kept for 25~28 DEG C, dark or have weaker scattered light；Cultivate and examined after 4~6d
Strain sprouting situation is looked into, mending in time for not sprouting checks pollution after inoculation, inoculation 10d, discovery pollution cleaning in time.
9. Hericium erinaceus culture method according to claim 1, it is characterised in that：The fruiting pipe of bacterium rod described in step (6)
Reason pulls out the bacterium nail of inoculation mouth, 25 DEG C of keeping temperature, allows after the mycelia of inoculation mouth recovers 3~5 days, then move out of mushroom shed to and go out
Mushroom.
10. Hericium erinaceus culture method according to claim 1, it is characterised in that the environmental condition described in step (7) is adjusted
Control also includes：
Fructification is especially sensitive to carbon dioxide during ventilation regulation and control, fruiting, now requires mushroom house well-ventilated, keeps air new
It is fresh, but cold wind can not be allowed to be directly blown onto in fructification；
Illumination regulates and controls, and mushroom house seven second of holding, three points of positive scattered lights were beneficial to the development of hericium erinaceus fruiting body.
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| CN114793751B (en) * | 2021-01-29 | 2023-12-22 | 天津市农业科学院 | Cultivation method of oyster mushrooms |
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