CN104718996B - The preparation method of edible mushroom solid liquefaction strain - Google Patents
The preparation method of edible mushroom solid liquefaction strain Download PDFInfo
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- CN104718996B CN104718996B CN201510172303.6A CN201510172303A CN104718996B CN 104718996 B CN104718996 B CN 104718996B CN 201510172303 A CN201510172303 A CN 201510172303A CN 104718996 B CN104718996 B CN 104718996B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 75
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 238000011081 inoculation Methods 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000001963 growth medium Substances 0.000 claims abstract description 14
- 238000011169 microbiological contamination Methods 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims description 44
- 239000000463 material Substances 0.000 claims description 28
- 238000004659 sterilization and disinfection Methods 0.000 claims description 22
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
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- 241000222351 Pleurotus cornucopiae Species 0.000 claims description 9
- 241000233866 Fungi Species 0.000 claims description 6
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 5
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims description 5
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- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
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- 235000009566 rice Nutrition 0.000 claims description 5
- 240000008397 Ganoderma lucidum Species 0.000 claims description 4
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims description 4
- 240000000588 Hericium erinaceus Species 0.000 claims description 4
- 235000007328 Hericium erinaceus Nutrition 0.000 claims description 4
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- 235000014102 seafood Nutrition 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
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- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention relates to mushroom planting technique field, more particularly to a kind of preparation method of edible mushroom solid liquefaction strain, level liquid strain is inoculated in blake bottle and cultivates to obtain liquid strain；Solid culture medium is prepared, the liquid strain of gained is cultivated in seed bottle, and cultivation temperature is 22 25 DEG C, lucifuge culture, and culture humidity is 50 60%, and 20 30 days strains of culture can cover with bacterium bottle；The bacterium bottle for covering with no miscellaneous bacteria is selected, strain is crushed in pulverizer, is inoculated into sterilized water, directly production inoculation.Strain preparation method of the present invention, has that bacterium germination is fast, can faster than solid spawn bacterium germination 1/3, it is not necessary to connect by 34 expansion, reduce the microbiological contamination problem expanded in termination process, saved expansion numerous required time；Growing point is more than common liq growth point, and bacterium germination is consistent, and cell age is than more consistent, and fruiting can be relatively more neat, and quality is good, and fruiting body yield can improve 20 30%；Mycelia is liquefied using sterilized water, the miscellaneous bacteria such as mould will not also grow, and pollution can reduce 5 10%.
Description
Technical field
The present invention relates to mushroom planting technique field, more particularly to a kind of preparation method of edible mushroom solid liquefaction strain.
Background technology
At present, edible fungus species are divided into two kinds of solid kind and liquid strain, and solid kind is simple to operate, easily observation, Neng Gouyou
Effect prevents the strain that access is polluted, but solid spawn also has the slow characteristic of bacterium germination, yields poorly, fruiting is irregular, bacterium germination rank
Duan Rongyi infects miscellaneous bacteria.Traditional liquid spawn makes one-level kind, it is necessary to be connect by 3-4 expansion by shaking table, is subsequently used for connecing
Kind, it can effectively accelerate bacterium germination speed, but complex operation with this liquid spawn, the problem of big batch pollution easily occur.
The content of the invention
The present invention the advantages of retaining original strain of the invention, changes for solid spawn and the advantage and disadvantage of traditional liquid strain
Enter shortcoming, it is proposed that a kind of preparation method of solid liquefaction strain.
The technical scheme is that：
A kind of preparation method of edible mushroom solid liquefaction strain, comprises the following steps：
Step 1：Level liquid strain is inoculated in blake bottle and cultivated, quiescent culture 2-3 days at 22-25 DEG C, fungus block life
It is long normal, oscillator rotating speed 200r/min is adjusted in the case of no microbiological contamination, culture produces liquid strain in 5-10 days；
Step 2：Solid culture medium is prepared, the liquid strain of the gained of inoculation step 1 is cultivated in seed bottle, and cultivation temperature is
22-25 DEG C, lucifuge culture, culture humidity is 50-60%, and 20-30 days strains of culture can cover with bacterium bottle；
Step 3：The bacterium bottle for covering with no miscellaneous bacteria is selected, strain is crushed in pulverizer, is inoculated into sterilized water, directly
Production inoculation.
On the basis of above scheme, the nutrient solution of the liquid spawn described in step 1 is：1L culture material formulas are：Soil
Beans 200g, wheat bran 20g, glucose 20g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g.
On the basis of above scheme, the solid culture medium described in step 2 mixes according to following mass percent, 1mm
Following sawdust 60-70%, wheat bran 15-20%, rice bran 5-10%, maize flour 5%, precipitated calcium carbonate 1-1.5%, gypsum 1-2%,
Lime 1-1.5%.
Wherein preferable, the seed bottle described in step 2 uses 500ml clear glass seed bottles, and lid uses air-permeable bottle
Lid, work loading height are 2-3cm below bottleneck；A diameter 1-1.5cm, the hole away from bottom of bottle 2-4cm are made a call in center.
Preferably, after the strain described in step 3 crushes, by 100 mesh screens, inoculate in sterilized water.
Preferably, the sterilized water described in step 3 is 121 DEG C by sterilising temp, and sterilization time is going out for 30-40min
Bacterium.
Preferably, described edible mushroom is that described edible mushroom is flat mushroom, pleurotus cornucopiae, elegant precious mushroom, white beech mushroom, crab flavour mushroom, sea
Fresh mushroom, Hericium erinaceus, elm mushroom, ganoderma lucidum.
The beneficial effects of the invention are as follows：
The present invention retains the advantages of original solid spawn and liquid spawn, its shortcoming is improved, by solid spawn and liquid bacteria
Kind, which is combined, carries out strain production, carries out rejuvenation to strain by changing culture medium, keeps the vigor of strain, prevent its degeneration,
According to the characteristics of mycelia apical growth, it is crushed, strain is expanded using the characteristics of strain apical growth after crushing
Germination point, therefore can change fluid nutrient medium makes it to be further cultured for so that strain can bacterium germination it is consistent, cell age is relative
Unanimously, and in the manufacturing process of strain traditional fluid nutrient medium is changed, even if a small amount of strain of seeded process is splashed down in bacterium
Kind bag is outer due to the change of culture medium, does not also allow to be also easy to produce miscellaneous bacteria, reduces pollution.
1) have bacterium germination it is fast, can faster than solid spawn bacterium germination 1/3, while solve the problems, such as liquid spawn expand connect, no
Need to connect by 3-4 expansion, reduce the microbiological contamination problem expanded in termination process, saved expansion numerous required time；
2) the solid culture stage of the present invention is the rejuvenation to mycelia, and mycelial growth is energetic, and mycelia is flowed after liquefaction
Property it is strong, growing point is more than common liq growth point, and bacterium germination is consistent, and for cell age than more consistent, fruiting can be relatively neat, and quality
Good, fruiting body yield can improve 20-30%；
3) mycelia is liquefied using sterilized water, sterilized water is substantially free of nutriment, for drippage when production is inoculated with
Because culture medium is that water does not provide the miscellaneous bacterias such as nutrition, mould and will not also grown, pollution can be reduced for strain outside inoculation mouth
5-10%.
Embodiment
The embodiment of the present invention is as follows：
Embodiment 1：Flat mushroom
First, flat mushroom strain is prepared,
The preparation method of flat mushroom solid liquefaction strain, comprises the following steps：
Step 1：By the inoculation of level liquid strain, (ultraviolet lamp disinfection 20min, is inoculated with superclean bench before inoculation, often
The strain block that bottle inoculation 4-5 blocks area is 3mm*3mm) cultivated in blake bottle, quiescent culture 2-3 days at 22-25 DEG C, fungus block life
It is long normal, oscillator rotating speed 200r/min is adjusted in the case of no microbiological contamination, culture produces liquid strain in 5-10 days；Described in step 1
The nutrient solution of liquid spawn be：1L culture material formulas are：Potato 200g, wheat bran 20g, glucose 20g, potassium dihydrogen phosphate 1g,
Magnesium sulfate 0.5g.It is dispensed into 1000ml shaking flasks, every bottle of compost 200-300ml, is gone out under the conditions of 121 DEG C after nutrient solution is cooked
Bacterium 30min.
Step 2：Solid culture medium is prepared, the liquid strain of the gained of inoculation step 1 is cultivated in seed bottle, institute in step 2
The solid culture medium stated mixes according to following mass percent, below 1mm sawdusts 68%, wheat bran 18%, rice bran 6.5%, corn
Face 5%, precipitated calcium carbonate 1%, gypsum 1%, lime 0.5%.Seed bottle uses 500ml clear glass seed bottles, and lid uses
Venting bottle cap, work loading height are 2-3cm below bottleneck.Diameter 1-1.5cm or so, the hole away from bottom of bottle 2-4cm are made a call in center.
The seed bottle made is put in autoclave, sterilize 90min under the conditions of 121 DEG C.Sterilizing, which finishes, is cooled to room temperature, net at hundred grades
The solid liquefaction strain made, every bag of inoculation bacterium solution 30-40ml are inoculated with the conditions of change.Cultivation temperature is 22-25 DEG C, lucifuge training
Support, culture humidity is 50-60%, and 20-30 days strains of culture can cover with bacterium bottle.
Step 3：The bacterium bottle for covering with no miscellaneous bacteria is selected, strain is crushed in pulverizer, is inoculated into sterilized water, directly
Production inoculation.Described sterilized water is 121 DEG C by sterilising temp, and sterilization time is 30-40min sterilizing.
10000 bags of flat mushroom is inoculated with the double net double film booths of Qingdao Hua Shenglvneng agricultural science and technologys Co., Ltd using liquid spawn
Carry out fruiting experiment, pleurotus cornucopiae bacterium bag be polyethylene 22*35cm, and it is 25cm or so that planting material, which loads height, fill after planting material
2cm*2.5cm hole is made a call in center, tying, carries out normal-pressure sterilization 18h, stewing to put 8h.
20 days or so mycelia of culture can cover with bacterium bag, shift to an earlier date 10-15 days than prior art, bacterium germination moves into photovoltaic after terminating
Sun-cloudiness shed carries out fruiting, and in standardized " ten " word osculum of each sack, temperature is 15-18 DEG C, humidity 85-95%, 15 days left sides
The right side can harvest first batch, go out second batch of mushroom again after bacteria.By testing, the flat mushroom for the cultivating bacterial spawn that liquefied using solid,
Biological transformation ratio is up to 220-300%, than prior art output increased 20-30%.
Embodiment 2：Pleurotus cornucopiae
First, pleurotus cornucopiae strain is prepared,
The preparation method of pleurotus cornucopiae solid liquefaction strain, comprises the following steps：
Step 1：By the inoculation of level liquid strain, (ultraviolet lamp disinfection 20min, is inoculated with superclean bench before inoculation, often
The strain block that bottle inoculation 4-5 blocks area is 3mm*3mm) cultivated in blake bottle, quiescent culture 2-3 days at 22-25 DEG C, fungus block life
It is long normal, oscillator rotating speed 200r/min is adjusted in the case of no microbiological contamination, culture produces liquid strain in 5-10 days；Described in step 1
The nutrient solution of liquid spawn be：1L culture material formulas are：Potato 200g, wheat bran 20g, glucose 20g, potassium dihydrogen phosphate 1g,
Magnesium sulfate 0.5g.It is dispensed into 1000ml shaking flasks, every bottle of compost 200-300ml, is gone out under the conditions of 121 DEG C after nutrient solution is cooked
Bacterium 30min.
Step 2：Solid culture medium is prepared, the liquid strain of the gained of inoculation step 1 is cultivated in seed bottle, institute in step 2
The solid culture medium stated mixes according to following mass percent, below 1mm sawdusts 70%, wheat bran 15%, rice bran 7.5%, corn
Face 5%, precipitated calcium carbonate 1%, gypsum 1%, lime 0.5%.Seed bottle uses 500ml clear glass seed bottles, and lid uses
Venting bottle cap, work loading height are 2-3cm below bottleneck.Diameter 1-1.5cm or so, the hole away from bottom of bottle 2-4cm are made a call in center.
The seed bottle made is put in autoclave, sterilize 90min under the conditions of 121 DEG C.Sterilizing, which finishes, is cooled to room temperature, net at hundred grades
The solid liquefaction strain made, every bag of inoculation bacterium solution 30-40ml are inoculated with the conditions of change.Cultivation temperature is 22-25 DEG C, lucifuge training
Support, culture humidity is 50-60%, and 20-30 days strains of culture can cover with bacterium bottle.
Step 3：The bacterium bottle for covering with no miscellaneous bacteria is selected, strain is crushed in pulverizer, by 100 mesh screens, connect
Kind is into sterilized water, directly production inoculation.Described sterilized water is 121 DEG C by sterilising temp, sterilization time 30-40min
Sterilizing.
Wrapped using solid liquefaction strain inoculation pleurotus cornucopiae 10000 in Qingdao Hua Shenglvneng agricultural science and technologys Co., Ltd beam-connected greenhouse
Carry out fruiting experiment, pleurotus cornucopiae bacterium bag be polyethylene 22*35cm, and it is 25cm or so that planting material, which loads height, fill after planting material
2cm*2.5cm hole is made a call in center, tying, carries out normal-pressure sterilization 18h, stewing to put 8h.20 days or so mycelia of culture can grow
Full bacterium bag, shifts to an earlier date 10-15 days than prior art.
Bacterium germination moves into photovoltaic beam-connected greenhouse after terminating and carries out fruiting, takes one end to open a bag fruiting, temperature is 16-18 DEG C, wet
Spend for 85-95%, first batch can be harvested within 15 days or so, go out second batch of mushroom again after bacteria.By experiment, using solid
The pleurotus cornucopiae of liquefaction cultivating bacterial spawn, biological transformation ratio is up to 150-180%, than prior art output increased 20-30%.
Embodiment 3：Elegant precious mushroom
The preparation method of elegant precious mushroom solid liquefaction strain, comprises the following steps：
Step 1：By the inoculation of level liquid strain, (ultraviolet lamp disinfection 20min, is inoculated with superclean bench before inoculation, often
The strain block that bottle inoculation 4-5 blocks area is 3mm*3mm) cultivated in blake bottle, quiescent culture 2-3 days at 22-25 DEG C, fungus block life
It is long normal, oscillator rotating speed 200r/min is adjusted in the case of no microbiological contamination, culture produces liquid strain in 5-10 days；Described in step 1
The nutrient solution of liquid spawn be：1L culture material formulas are：Potato 200g, wheat bran 20g, glucose 20g, potassium dihydrogen phosphate 1g,
Magnesium sulfate 0.5g.It is dispensed into 1000ml shaking flasks, every bottle of compost 200-300ml, is gone out under the conditions of 121 DEG C after nutrient solution is cooked
Bacterium 30min.
Step 2：Solid culture medium is prepared, the liquid strain of the gained of inoculation step 1 is cultivated in seed bottle, institute in step 2
The solid culture medium stated mixes according to following mass percent, below 1mm sawdusts 72%, wheat bran 15%, rice bran 5.5%, corn
Face 5%, precipitated calcium carbonate 1%, gypsum 1%, lime 0.5%.Seed bottle uses 500ml clear glass seed bottles, and lid uses
Venting bottle cap, work loading height are 2-3cm below bottleneck.Diameter 1-1.5cm or so, the hole away from bottom of bottle 2-4cm are made a call in center.
The seed bottle made is put in autoclave, sterilize 90min under the conditions of 121 DEG C.Sterilizing, which finishes, is cooled to room temperature, net at hundred grades
Change the solid liquefaction strain for being inoculated with and making under aseptic condition, every bag of inoculation bacterium solution 30-40ml.Cultivation temperature is 22-25 DEG C,
Lucifuge culture, culture humidity are 50-60%, and 20-30 days strains of culture can cover with bacterium bottle.
Step 3：The bacterium bottle for covering with no miscellaneous bacteria is selected, strain is crushed in pulverizer, by 100 mesh screens, connect
Kind is into sterilized water, directly production inoculation.Described sterilized water is 121 DEG C by sterilising temp, sterilization time 30-40min
Sterilizing.
Elegant precious mushroom bacterium bag 5000 is made using solid liquefaction strain in Qingdao Hua Shenglvneng agricultural science and technologys Co., Ltd to wrap, it is elegant
Precious mushroom bag is polypropylene plastics pocket 22*35cm, and planting material loads height as 25cm or so, fills and makes a call to one in center after planting material
Individual 2cm*2.5cm hole, tying, carry out autoclaving, 121 DEG C of sterilizing 3h.Culture mycelia can cover with for 15-20 days or so
Bacterium bag, shift to an earlier date 10-15 days than prior art.Bacterium germination moves into Jimo City Pu Dong towns peasant household greenhouse and carries out fruiting after terminating, each
Standardized " ten " word osculum of sack, temperature is 16-18 DEG C, humidity 80-85%, can harvest within 20-23 days or so first batch, warp
Cross Yang Junhou and go out second batch of mushroom again.It is reachable through overtesting, the elegant precious mushroom for the cultivating bacterial spawn that liquefied using solid, biological transformation ratio
100-120%, 30% or so is improved than prior art.
Embodiment 4：Crab flavour mushroom
In Qingdao Hua Shenglvneng agricultural science and technologys Co., Ltd make 3000 bottles of crab flavour mushroom, preparation method as described in Example 1,
Bacterium bottle is polypropylene material, and volume 1100ml, bottleneck diameter 85mm, planting material is highly 1cm or so under bottleneck, fills cultivation
Training material bonnet upper bottle cover, autoclaving is carried out, sterilising temp is 121 DEG C, sterilization time 3h.Culture is entered in intelligent bacteria room
Row bacteria management, fruiting experiment carry out fruiting, design parameter such as following table in intelligent mushroom room：
| Strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| It is inoculated with quantity | 1000 | 1000 | 1000 |
| Sterilising temp (DEG C) | 121 | 121 | 121 |
| Sterilization time (h) | 3 | 3 | 3 |
| Cool time (h) | 24 | 24 | 24 |
| It is inoculated with strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| Inoculum concentration | 20g | 20ml | 20ml |
| Cultural hypha temperature (DEG C) | 22-24 | 22-24 | 22-24 |
| Cultural hypha humidity (%) | 60-70 | 60-70 | 60-70 |
| The cultural hypha time | 85 | 80 | 75 |
| Pollution rate (%) | 0.5 | 1 | 0.2 |
| Fruiting temperature (%) | 10-18 | 10-18 | 10-18 |
| Cultivate humidity (%) | 85-95 | 85-95 | 85-95 |
| Divulge information (on/off) | 5/10 | 5/10 | 5/10 |
| Illumination (lux) | 200-500 | 200-500 | 200-500 |
| Fruiting phase (my god) | 23 | 23 | 23 |
| Yield (g/ bottles) | 125 | 140 | 170 |
Through experiment, output increased 21.4-36%, Contamination rate control greatly reduces pollution rate 0.2%.
Embodiment 5：White beech mushroom
In Qingdao Hua Shenglvneng agricultural science and technologys Co., Ltd make 3000 bottles of white beech mushroom, preparation method as described in Example 1,
Bacterium bottle is polypropylene material, and volume 1100ml, bottleneck diameter 85mm, planting material is highly 1cm or so under bottleneck, fills cultivation
Training material bonnet upper bottle cover, autoclaving is carried out, sterilising temp is 121 DEG C, sterilization time 3h.Culture is entered in intelligent bacteria room
Row bacteria management, fruiting experiment carry out fruiting, design parameter such as following table in intelligent mushroom room：
| Strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| It is inoculated with quantity | 1000 | 1000 | 1000 |
| Sterilising temp (DEG C) | 121 | 121 | 121 |
| Sterilization time (h) | 3 | 3 | 3 |
| Cool time (h) | 24 | 24 | 24 |
| It is inoculated with strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| Inoculum concentration | 20g | 20ml | 20ml |
| Cultural hypha temperature (DEG C) | 22-24 | 22-24 | 22-24 |
| Cultural hypha humidity (%) | 60-70 | 60-70 | 60-70 |
| The cultural hypha time | 85 | 80 | 75 |
| Pollution rate (%) | 0.5 | 1 | 0.2 |
| Fruiting temperature (%) | 8-16 | 8-16 | 8-16 |
| Cultivate humidity (%) | 85-95 | 85-95 | 85-95 |
| Divulge information (on/off) | 5/10 | 5/10 | 5/10 |
| Illumination (lux) | 200-500 | 200-500 | 200-500 |
| Fruiting phase (my god) | 23 | 23 | 23 |
| Yield (g/ bottles) | 120 | 130 | 160 |
Through experiment, output increased 23.1-33.3%, Contamination rate control greatly reduces pollution rate 0.2%.
Embodiment 6：Seafood mushroom
In Qingdao Hua Shenglvneng agricultural science and technologys Co., Ltd make 3000 bags of white beech mushroom, preparation method as described in Example 1,
Bacterium bottle is polypropylene material, specification 17*33cm, planting material is highly 20cm or so under bottleneck, is filled after planting material in center
16cm perforation rod is inserted, covers bottle cap, carries out autoclaving, sterilising temp is 121 DEG C, sterilization time 3h.Culture is in intelligence
Bacteria room can be changed and carry out bacteria management, fruiting experiment carries out fruiting, design parameter such as following table in intelligent mushroom room：
| Strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| It is inoculated with quantity (bag) | 1000 | 1000 | 1000 |
| Sterilising temp (DEG C) | 121 | 121 | 121 |
| Sterilization time (h) | 3 | 3 | 3 |
| Cool time (h) | 24 | 24 | 24 |
| It is inoculated with strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| Inoculum concentration | 30-40g | 30ml | 30ml |
| Cultural hypha temperature (DEG C) | 22-25 | 22-25 | 22-25 |
| Cultural hypha humidity (%) | 60-70 | 60-70 | 60-70 |
| The cultural hypha time | 90 | 85 | 80 |
| Pollution rate (%) | 0.5 | 1 | 0.2 |
| Fruiting temperature (%) | 8-16 | 8-16 | 8-16 |
| Cultivate humidity (%) | 85-95 | 85-95 | 85-95 |
| Divulge information (on/off) | 5/10 | 5/10 | 5/10 |
| Illumination (lux) | 200-500 | 200-500 | 200-500 |
| Fruiting phase (my god) | 25 | 24 | 24 |
| Yield (g/ bottles) | 120 | 130 | 160 |
Through experiment, output increased 23.1-33.3%, Contamination rate control greatly reduces pollution rate 0.2%.
Embodiment 7：Hericium erinaceus
In Qingdao Hua Shenglvneng agricultural science and technologys Co., Ltd make 3000 bags of Hericium erinaceus, preparation method as described in Example 2,
Bacterium bottle is polypropylene material, specification 17*33cm, planting material is highly 20cm or so, is inserted after filling planting material in center
16cm perforation rod, is sealed with three-piece, carries out autoclaving, and sterilising temp is 121 DEG C, sterilization time 3h.Culture is in intelligence
Bacteria room can be changed and carry out bacteria management, fruiting experiment is carried out in photovoltaic tunnel greenhouse, design parameter such as following table：
| Strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| It is inoculated with quantity (bag) | 1000 | 1000 | 1000 |
| Sterilising temp (DEG C) | 121 | 121 | 121 |
| Sterilization time (h) | 3 | 3 | 3 |
| Cool time (h) | 24 | 24 | 24 |
| It is inoculated with strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| Inoculum concentration | 30-40g | 30ml | 30ml |
| Cultural hypha temperature (DEG C) | 22-25 | 22-25 | 22-25 |
| Cultural hypha humidity (%) | 60-70 | 60-70 | 60-70 |
| The cultural hypha time | 30 | 25 | 20 |
| Pollution rate (%) | 0.3 | 0.5 | 0.1 |
| Fruiting temperature (%) | 16-20 | 16-20 | 16-20 |
| Cultivate humidity (%) | 85-95 | 85-95 | 85-95 |
| Illumination (lux) | 300-500 | 300-500 | 300-500 |
| Fruiting phase (my god) | 20 | 20 | 20 |
| Biological transformation ratio (%) | 100 | 110 | 120 |
Through experiment, bacterium germination time advance can cover with bacterium bag for 5-10 days, and biological transformation ratio improves 10-20%, pollution rate
Control greatly reduces pollution rate within 0.1%.
Embodiment 8：Elm mushroom
In Qingdao, Hua Shenglvneng agricultural science and technologys Co., Ltd makes 3000 bags of elm mushroom, preparation method such as embodiment 2, bacterium bottle
For polypropylene material, specification 22*38cm, planting material is highly 25cm or so, is filled after planting material center insertion 20cm's
Perforation rod, sealed with three-piece, carry out autoclaving, sterilising temp is 121 DEG C, sterilization time 3h.Culture is supported in intellectuality
Bacterium room carries out bacteria management, and fruiting experiment is carried out in photovoltaic tunnel greenhouse, design parameter such as following table：
| Strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| It is inoculated with quantity (bag) | 1000 | 1000 | 1000 |
| Sterilising temp (DEG C) | 121 | 121 | 121 |
| Sterilization time (h) | 3 | 3 | 3 |
| Cool time (h) | 24 | 24 | 24 |
| It is inoculated with strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| Inoculum concentration | 30-40g | 30ml | 30ml |
| Cultural hypha temperature (DEG C) | 22-25 | 22-25 | 22-25 |
| Cultural hypha humidity (%) | 60-70 | 60-70 | 60-70 |
| The cultural hypha time | 35 | 30 | 25 |
| Pollution rate (%) | 1 | 2 | 0.5 |
| Fruiting temperature (%) | 18-25 | 18-25 | 18-25 |
| Cultivate humidity (%) | 85-95 | 85-95 | 85-95 |
| Illumination (lux) | 300-500 | 300-500 | 300-500 |
| Fruiting phase (my god) | 20 | 20 | 20 |
| Biological transformation ratio (%) | 120 | 115 | 130 |
Through experiment, the bacterium germination time can shorten, and can shift to an earlier date the full bacterium bag of 5-10 days hairs, and biological transformation ratio improves 10-15%,
Contamination rate control greatly reduces pollution rate within 0.5%.
Embodiment 9：Ganoderma lucidum
In Qingdao, Hua Shenglvneng agricultural science and technologys Co., Ltd makes 3000 bags of ganoderma lucidum, preparation method such as embodiment 2, and bacterium bottle is
Polypropylene material, specification 17*33cm, planting material is highly 20cm or so, fills beating in center insertion 20cm after planting material
Hole rod, is sealed with three-piece, carries out autoclaving, and sterilising temp is 121 DEG C, sterilization time 3h.Culture is in intelligent bacteria
Room carries out bacteria management, and fruiting experiment is carried out in photovoltaic tunnel greenhouse, design parameter such as following table：
| Strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| It is inoculated with quantity (bag) | 1000 | 1000 | 1000 |
| Sterilising temp (DEG C) | 121 | 121 | 121 |
| Sterilization time (h) | 3 | 3 | 3 |
| Cool time (h) | 24 | 24 | 24 |
| It is inoculated with strain | Solid spawn | Liquid spawn | Solid liquefaction strain |
| Inoculum concentration | 30-40g | 30ml | 30ml |
| Cultural hypha temperature (DEG C) | 25-30 | 25-30 | 25-30 |
| Cultural hypha humidity (%) | 50-60 | 50-60 | 50-60 |
| Divulge information (min/d) | 30 | 40 | 40 |
| The cultural hypha time | 35 | 28 | 23 |
| Pollution rate (%) | 1 | 2 | 0.5 |
| Fruiting temperature (%) | 25-28 | 25-28 | 25-28 |
| Cultivate humidity (%) | 85-95 | 85-95 | 85-95 |
| Illumination (lux) | 500-1000 | 500-1000 | 500-1000 |
| Fruiting phase (my god) | 45 | 43 | 40 |
| Biological transformation ratio (%) | 80 | 90 | 95 |
Through experiment, the bacterium germination time shortens 5-8 days, and biological transformation ratio improves 5-11%, Contamination rate control 0.5% with
It is interior, greatly reduce pollution rate.
Claims (4)
1. a kind of preparation method of edible mushroom solid liquefaction strain, it is characterised in that comprise the following steps：
Step 1：Level liquid strain is inoculated in blake bottle and cultivated, quiescent culture 2-3 days at 22-25 DEG C, fungus block grows just
Often, oscillator rotating speed 200r/min is adjusted in the case of no microbiological contamination, culture produces liquid strain in 5-10 days；
The nutrient solution of described liquid spawn is：1L culture material formulas are：Potato 200g, wheat bran 20g, glucose 20g, di(2-ethylhexyl)phosphate
Hydrogen potassium 1g, magnesium sulfate 0.5g；
Step 2：Solid culture medium is prepared, the liquid strain of the gained of inoculation step 1 is cultivated in seed bottle, cultivation temperature 22-
25 DEG C, lucifuge culture, culture humidity is 50-60%, and 20-30 days strains of culture can cover with bacterium bottle；
Described solid culture medium mixes according to following mass percent, below 1mm sawdust 60-70%, wheat bran 15-20%, rice
Chaff 5-10%, maize flour 5%, precipitated calcium carbonate 1-1.5%, gypsum 1-2%, lime 1-1.5%；
Step 3：The bacterium bottle for covering with no miscellaneous bacteria is selected, strain is crushed in pulverizer, is inoculated into sterilized water, is directly produced
Inoculation；
Described edible mushroom is flat mushroom, pleurotus cornucopiae, elegant precious mushroom, white beech mushroom, crab flavour mushroom, seafood mushroom, Hericium erinaceus, elm mushroom, ganoderma lucidum.
2. the preparation method of edible mushroom solid liquefaction strain according to claim 1, it is characterised in that described in step 2
Seed bottle use 500ml clear glass seed bottles, lid uses venting bottle cap, and work loading height is 2-3cm below bottleneck；Center
Make a call to a diameter 1-1.5cm, the hole away from bottom of bottle 2-4cm.
3. the preparation method of edible mushroom solid liquefaction strain according to claim 1, it is characterised in that described in step 3
Strain crush after, by 100 mesh screens, inoculate in sterilized water.
4. the preparation method of edible mushroom solid liquefaction strain according to claim 1, it is characterised in that described in step 3
Sterilized water by sterilising temp be 121 DEG C, sterilization time be 30-40min sterilizing.
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| CN106399123B (en) * | 2016-08-30 | 2019-12-20 | 中国农业科学院农业资源与农业区划研究所 | Method for quickly producing seeds of edible fungi |
| CN108285873A (en) * | 2017-08-29 | 2018-07-17 | 上海雪榕生物科技股份有限公司 | A kind of preparation method of edible fungus solid spawn reduction liquid |
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| CN110036829A (en) * | 2019-04-24 | 2019-07-23 | 中国农业科学院农业资源与农业区划研究所 | A kind of preparation method of oyster mushroom solid liquefaction strain |
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| CN114208590A (en) * | 2021-12-22 | 2022-03-22 | 黄爱敏 | Preparation method of novel edible fungus culture medium |
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