CN103833443A

CN103833443A - Needle mushroom-strain liquidation culture medium

Info

Publication number: CN103833443A
Application number: CN201210526265.6A
Authority: CN
Inventors: 陶军
Original assignee: TIANSHUI ZHONGXING BIO-TECHNOLOGY Co Ltd
Current assignee: TIANSHUI ZHONGXING BIO-TECHNOLOGY Co Ltd
Priority date: 2012-11-27
Filing date: 2012-11-27
Publication date: 2014-06-04

Abstract

The invention discloses a needle mushroom-strain liquidation culture medium. A solid strain cultured by utilizing the culture medium can be directly made into a liquid strain. The culture medium is prepared from raw materials and water according to a weight ratio of 1:(1.2-1.3), wherein the raw materials include the following components in percentage by weight: 45-55% of paper pulp, 36-44% of bagasse, 5.4-6.6% of corn flour, 0.9-1.1% of sugar, 0.9-1.1% of plaster, 0.9-1.1% lime of and 0.9-1.1% of calcium superphosphate.

Description

A kind of needle mushroom bacterial classification liquefaction substratum

Technical field

The present invention relates to a kind of needle mushroom bacterial classification substratum, relate in particular to a kind of needle mushroom bacterial classification liquefaction substratum.

Background technology

Solid spawn has the shortcomings such as growth cycle is long, cell age is inconsistent, of poor quality, and liquid spawn has and is difficult for transport, storing, detect, pick the shortcomings such as assorted and cost of investment is higher, becomes the principal element that restriction factory edible fungi develops; The technology of utilizing solid spawn directly to expand liquid spawn processed is that needle mushroom bacterial classification liquefaction technology has solved liquid spawn and is difficult for transport, store, detect, pick and mixes and the shortcoming such as cost of investment is higher, have again that liquid spawn fruiting is neat, growth time is fast, output advantages of higher, the selection of substratum of wherein liquefying is gordian technique, solid spawn by this culture medium culturing can directly be expanded and be made liquid spawn, it has directly determined the success or failure of needle mushroom bacterial classification liquefaction, and there is no in the prior art the record of needle mushroom bacterial classification liquefaction correlation technique.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of needle mushroom bacterial classification liquefaction substratum, can directly be expanded and make liquid spawn by the solid spawn of this culture medium culturing.

For solving the problems of the technologies described above, technical scheme of the present invention is: a kind of needle mushroom bacterial classification liquefaction substratum, this substratum by raw material, water by 1: the weight ratio of 1.2-1.3 forms, the compositions in weight percentage of wherein said raw material is: paper pulp 45-55%, bagasse 36-44%, Semen Maydis powder 5.4-6.6%, sugar, gypsum, lime, the each 0.9-1.1% of calcium superphosphate.

Preferably, the compositions in weight percentage of described raw material is: paper pulp 50%, bagasse 40%, Semen Maydis powder 6%, sugar, gypsum, lime, calcium superphosphate each 1%.

The invention has the beneficial effects as follows, the formula that substratum adopts is reasonable, be applicable to solid spawn liquefaction, liquefied residue is few, and liquefaction effect reaches more than 98%, bacterial strain mycelia phene is good, mycelium germination power is strong, and colonial morphology is neat, mycelia is pure white, dense, mycelial growth rate 0.97cm/d, preparation technology is simple to operate, is applicable to batch production operation; It is the shortest 41.8 days that factory culture needle mushroom is inoculated into the number of days of gathering, output and biological efficiency are the highest, be respectively 335.8g/ bottle (the every bottled substratum 850g of entering of culture bottle) and 105% (the fresh amount/culture material of biological efficiency=sporophore dry weight × 100%), mushroom reguarity is good.

Embodiment

Embodiment 1

A kind of needle mushroom bacterial classification liquefaction substratum, this substratum is made up of by the weight ratio of 1: 1.2 raw material, water, and the compositions in weight percentage of wherein said raw material is: paper pulp 45%, bagasse 44%, Semen Maydis powder 6.6%, sugar, gypsum, lime, calcium superphosphate each 1.1%.

Needle mushroom bacterial classification liquefaction making method comprises the steps: 1) to cultivate slant medium be PDA enriched medium to test tube: potato 200g, Semen Maydis powder 20g, wheat bran 50g, glucose 20g, KH ₂pO ₄1g, MgSO ₄1g, VB ₁10, agar 20g, water 1000ml; By on the inclined-plane of F8815 bacterial strain (Institute of Plant Protection of the Chinese Academy of Agricultural Sciences) access PDA enriched medium, put 25 ℃ of constant temperature culture 8-10 days; 2) vial is bottled after cultivating described liquefaction substratum mixed preparing, at 1.5kg/cm ³sterilizing 1.5 hours at pressure, 121 ℃ of temperature, in the time that temperature is down to below 30 ℃, the mother who forms after bacterial strain test tube is cultivated plants in access bottle, puts at 23 ℃ of temperature, to be cultured to mycelia and to send out full; 3) step 2 is got in liquefaction) in the culture (original seed) that produces be placed in fermentor tank sterilized water by culture (original seed) bacterium piece, the sterilized water weight ratio of 1: 500 after pulverizing, stir evenly.

Embodiment 2

A kind of needle mushroom bacterial classification liquefaction substratum, this substratum is made up of by the weight ratio of 1: 1.3 raw material, water, and the compositions in weight percentage of wherein said raw material is: paper pulp 50%, bagasse 40%, Semen Maydis powder 6%, sugar, gypsum, lime, calcium superphosphate each 1%.

Needle mushroom bacterial classification liquefaction making method is identical in embodiment 1, repeats no more.

Embodiment 3

A kind of needle mushroom bacterial classification liquefaction substratum, this substratum is made up of by the weight ratio of 1: 1.25 raw material, water, and the compositions in weight percentage of wherein said raw material is: paper pulp 55%, bagasse 36%, Semen Maydis powder 5.4%, sugar, gypsum, lime, calcium superphosphate each 0.9%.

Claims

1. a needle mushroom bacterial classification liquefaction substratum, it is characterized in that, this substratum by raw material, water by 1: the weight ratio of 1.2-1.3 forms, the compositions in weight percentage of wherein said raw material is: paper pulp 45-55%, bagasse 36-44%, Semen Maydis powder 5.4-6.6%, sugar, gypsum, lime, the each 0.9-1.1% of calcium superphosphate.

2. needle mushroom bacterial classification liquefaction substratum according to claim 1, is characterized in that, the compositions in weight percentage of described raw material is: paper pulp 50%, bagasse 40%, Semen Maydis powder 6%, sugar, gypsum, lime, calcium superphosphate each 1%.