CN103497055B - Pleurotus eryngii culture medium utilizing miscanthus sinensis as matrix and preparation method thereof - Google Patents

Pleurotus eryngii culture medium utilizing miscanthus sinensis as matrix and preparation method thereof Download PDF

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Publication number
CN103497055B
CN103497055B CN201310457230.6A CN201310457230A CN103497055B CN 103497055 B CN103497055 B CN 103497055B CN 201310457230 A CN201310457230 A CN 201310457230A CN 103497055 B CN103497055 B CN 103497055B
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chinese silvergrass
matrix
culture medium
pleurotus eryngii
preparation
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CN201310457230.6A
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CN103497055A (en
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易自力
夏志兰
罗坤
彭程
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Hunan Agricultural University
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Hunan Agricultural University
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Abstract

The invention provides a pleurotus eryngii culture medium utilizing miscanthus sinensis as a matrix and a preparation method thereof. The pleurotus eryngii culture medium comprises the following components by mass percentage: 3%-8% of bean pulp, 5%-9% of corn powder, 3%-9% of wheat bran, 72%-82% of miscanthus sinensis, 1.8%-2.2% of light calcium carbonate and 0.8%-1.2% of lime. The invention further provides the preparation method of the pleurotus eryngii culture medium. The pleurotus eryngii culture medium which takes the miscanthus sinensis as a main part is used for cultivating the miscanthus sinensis; the biotransformation efficiency of the formula of the culture medium can be up to be more than 80%; the yield and the quality of pleurotus eryngii culture products can be improved and the production cost is reduced.

Description

A kind ofly utilize Chinese silvergrass as Xingbao mushroom medium of matrix and preparation method thereof
Technical field
The present invention relates to a kind of Xingbao mushroom medium and preparation method thereof, especially relate to and a kind ofly utilize Chinese silvergrass as Xingbao mushroom medium of matrix and preparation method thereof.
Background technology
Xingbao mushroom ( pleurotus eryngii), having another name called eryngo and pick up the ears, it is subject to consumer's favor deeply with good mouthfeel and longer shelf life.In recent years, the factory culture of this rare edible mushroom at home and abroad receives much concern.Research practice shows, the industrialized cultivation medium of Xingbao mushroom has direct impact to its output, quality and production cost etc., and therefore, selecting appropriate culture medium is the basis that Pleurotus eryngii industrial cultivation obtains more efficient benefit.
At present, it is main carbon source that the industrialized cultivation medium of Xingbao mushroom be take wood chip, cotton seed hulls, corncob etc., and wheat bran, rice bran, corn flour etc. are main nitrogen, and production cost is higher.
Summary of the invention
Technical problem to be solved by this invention is, provide a kind of production cost low utilize Chinese silvergrass as Xingbao mushroom medium of matrix and preparation method thereof.
The technical solution adopted for the present invention to solve the technical problems:
The present invention utilizes Chinese silvergrass as the Xingbao mushroom medium of matrix, comprises the component of following quality per distribution ratio: dregs of beans 3-8%, corn flour 5-9%, wheat bran 3-9%, Chinese silvergrass 72-82%, precipitated calcium carbonate 1.8-2.2%, lime 0.8-1.2%.
Further, dregs of beans 4-6%, corn flour 6-8%, wheat bran 4-7%, Chinese silvergrass 75-80%, precipitated calcium carbonate 1.8-2.2%, lime 0.8-1.2%.
Further, described Chinese silvergrass is Caulis Miscanthis floriduli, Nan Di, the assorted awns in Hunan No. 1, the assorted awns of the assorted awns in Hunan No. 2 or Hunan No. 3.
The present invention utilizes Chinese silvergrass as the preparation method of the Xingbao mushroom medium of matrix, comprises the following steps:
(1) pre-treatment of Chinese silvergrass: collect Chinese silvergrass stem stalk and blade, be dried to after water content < 10wt%, be crushed to 0.5 centimetre of particle diameter <; Or undried is directly crushed to 0.5 centimetre of particle diameter <;
(2) according to quality per distribution ratio dregs of beans 3-8%, corn flour 5-9%, wheat bran 3-9%, Chinese silvergrass 72-82%, precipitated calcium carbonate 1.8-2.2%, lime 0.8-1.2% takes each component, mixes.
By the spice pack according to a conventional method of gained medium, sterilizing, inoculation, after inoculation, in 20-26 ℃ of culturing room, send out bacterium and cultivate, after mycelia is covered with pocket, then through ripe cultivation in 29-32 days, enter management of producing mushroom.
Chinese silvergrass ( miscanthus) be the general designation of various awns platymisciums, containing having an appointment 15 to 20 species, belong to grass family.Assorted No. 1, No. 2, No. 3 three kinds of awns in Hunan that Agricultural University Of Hunan cultivates awns and southern reed artificial hybridization, its dry matter production per mu yield reaches 2500 kilograms, plant height can reach 4 meters, individual plant tiller number reaches more than 100, cellulose and hemicellulose reach 75%, total ash is below 5%, and annidation is strong, can vegetative propagation; Plant and can gather in for many years for 1 time, the assorted awns in Hunan No. 1, the assorted awns of the assorted awns in Hunan No. 2 and Hunan all reached high term No. 3, and maintain more than 5 years after 2 years.
Research shows, Chinese silvergrass has unique biological property, very high to luminous energy, moisture, N element availability, growth is fast, damage by disease and insect seldom, without using agricultural chemicals and chemical fertilizer, cheap, cellulose and hemicellulose level can meet the growth needs of Xingbao mushroom up to more than 80%, are to produce the organic or good host material of nuisanceless Xingbao mushroom.Use the present invention containing the Xingbao mushroom medium Xinbao mushroom culturing of Chinese silvergrass, Chinese silvergrass biological transformation ratio wherein can reach more than 80%, can improve Xingbao mushroom product yield and quality, reduces production costs.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
embodiment 1
The present embodiment utilize Chinese silvergrass as the Xingbao mushroom medium of matrix, comprise the component of following quality per distribution ratio: dregs of beans 5%, corn flour 8%, wheat bran 5%, Chinese silvergrass 79%(kind is the assorted awns in Hunan No. 1), precipitated calcium carbonate 2%, lime 1%.
Preparation method, comprises the following steps:
(1) pre-treatment of Chinese silvergrass: collect Chinese silvergrass stem stalk and blade, be dried to after water content < 10wt%, be crushed to 0.5 centimetre of particle diameter <;
(2) according to quality per distribution ratio dregs of beans 5%, corn flour 8%, wheat bran 5%, Chinese silvergrass 79%(kind is the assorted awns in Hunan No. 1), precipitated calcium carbonate 2%, lime 1% takes each component, mixes.
comparative example
Do not use Chinese silvergrass as the Xingbao mushroom medium of matrix, comprise the component of following quality per distribution ratio: dregs of beans 10%, corn flour 10%, wheat bran 15%, cotton seed hulls 38%, wood chip 24%, precipitated calcium carbonate 2%, lime 1%.
By embodiment 1-3 and comparative example gained medium, each is processed 100 bags of inoculations and carries out experiment in cultivation, every packed siccative 400g, spice pack according to a conventional method, sterilizing, inoculation, after inoculation, Zhi25℃ culturing room sends out bacterium and cultivates, after mycelia is covered with pocket, more enter management of producing mushroom through ripe cultivation in 30 days, bacterium, fruiting situation sent out in record.
The growth rate of table 1 different formulations mycelia
As shown in Table 1, with comparative example comparison, the embodiment of the present invention is in sprouting speed, day growth speed, on average cover with aspect bacterium bag time, output and have significant advantage, and hence one can see that, and Chinese silvergrass is of high nutritive value, and can be used as organic Xingbao mushroom production matrix.
embodiment 2-5
Chinese silvergrass kind is selected respectively as the assorted awns of the assorted awns of Caulis Miscanthis floriduli, Nan Di, Hunan No. 2 and Hunan No. 3, and all the other components and component proportion are with embodiment 1.
Each is processed 100 bags of inoculations and carries out experiment in cultivation, every packed siccative 400g, spice pack according to a conventional method, sterilizing, inoculation, after inoculation, Zhi25℃ culturing room sends out bacterium and cultivates, after mycelia is covered with pocket, through ripe cultivation in 30 days, enter management of producing mushroom again, bacterium, fruiting situation sent out in record.4 Chinese silvergrass kinds are all applicable to the mycelial growth of Xingbao mushroom, fruiting smoothly, and volume variance is not remarkable.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims (4)

1. utilize Chinese silvergrass as an Xingbao mushroom medium for matrix, it is characterized in that, comprise the component of following quality per distribution ratio: dregs of beans 3-8%, corn flour 5-9%, wheat bran 3-9%, Chinese silvergrass 72-82%, precipitated calcium carbonate 1.8-2.2%, lime 0.8-1.2%.
2. as claimed in claim 1ly utilize Chinese silvergrass as the Xingbao mushroom medium of matrix, it is characterized in that, comprise the component of following quality per distribution ratio: dregs of beans 4-6%, corn flour 6-8%, wheat bran 4-7%, Chinese silvergrass 75-80%, precipitated calcium carbonate 1.8-2.2%, lime 0.8-1.2%.
3. as claimed in claim 1 or 2ly utilize Chinese silvergrass as the Xingbao mushroom medium of matrix, it is characterized in that: described Chinese silvergrass is Caulis Miscanthis floriduli, Nan Di, the assorted awns in Hunan No. 1, the assorted awns of the assorted awns in Hunan No. 2 or Hunan No. 3.
4. as claimed in claim 1ly utilize Chinese silvergrass as a preparation method for the Xingbao mushroom medium of matrix, it is characterized in that, comprise the following steps:
(1) pre-treatment of Chinese silvergrass: collect Chinese silvergrass stem stalk and blade, be dried to after water content < 10wt%, be crushed to 0.5 centimetre of particle diameter <; Or undried is directly crushed to 0.5 centimetre of particle diameter <;
(2) according to quality per distribution ratio dregs of beans 3-8%, corn flour 5-9%, wheat bran 3-9%, Chinese silvergrass 72-82%, precipitated calcium carbonate 1.8-2.2%, lime 0.8-1.2% takes each component, mixes.
CN201310457230.6A 2013-09-30 2013-09-30 Pleurotus eryngii culture medium utilizing miscanthus sinensis as matrix and preparation method thereof Expired - Fee Related CN103497055B (en)

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CN103936502B (en) * 2014-04-04 2016-03-23 湖南农业大学 A kind of bisporous mushroom is cultivated by Chinese silvergrass matrix composition and using method
CN104140310B (en) * 2014-08-21 2016-08-24 湖南省宇秀生物科技有限公司 A kind of culture medium of factory culture Pleurotus eryngii
CN105210669B (en) * 2015-08-28 2018-07-06 湖南省致远农业科技发展有限公司 It is a kind of using wild Chinese silvergrass as the method for major ingredient factory culture ganoderma lucidum
CN108990758A (en) * 2018-09-25 2018-12-14 杭州师范大学 It a kind of roxburgh anoectochilus terminal bud seedling cultivation matrix and its prepares and seedling cultural method
CN110419388A (en) * 2019-08-02 2019-11-08 十堰市农业科学院 A kind of pleurotus eryngii cultivating material and preparation method based on sweet Chinese sorghum pole
CN110897083A (en) * 2019-11-04 2020-03-24 湖南博大天能实业股份有限公司 Amur triarrhena sacchariflora and bamboo shoot noodles and making method thereof
CN111713337A (en) * 2020-07-27 2020-09-29 黄冈师范学院 Organic cultivation method of stropharia rugoso-annulata by using wild Chinese silvergrass as main material
CN113951050A (en) * 2021-10-12 2022-01-21 中国科学院亚热带农业生态研究所 Oyster mushroom culture medium and preparation method thereof

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CN102807440B (en) * 2012-08-03 2014-06-25 福建省健神生物工程有限公司 Preparation method of cultivating substitute of lucid ganoderma

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