CN102498948B - Method for cultivating Chinese cryptoporus volvatus - Google Patents
Method for cultivating Chinese cryptoporus volvatus Download PDFInfo
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- CN102498948B CN102498948B CN 201110348368 CN201110348368A CN102498948B CN 102498948 B CN102498948 B CN 102498948B CN 201110348368 CN201110348368 CN 201110348368 CN 201110348368 A CN201110348368 A CN 201110348368A CN 102498948 B CN102498948 B CN 102498948B
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Abstract
The invention relates to a method for cultivating Chinese cryptoporus volvatus, comprising the following steps of: taking solid Chinese cryptoporus volvatus microorganism, which is cultivated at the temperature of 25 DEG C for 7 days, to be plum blossom-shaped multi-point inoculated to a fungus cultivation bag with cultivation substrates comprising 40-50 percent of needle leave sawdust, 25-35 percent of cottonseed hull, 15-20 percent of wheat bran, 0.5-1 percent of yeast powder for analysis, 0.5-1 percent of turpentine, 1-2 percent of gypsum, and 0.5-1 percent of superphosphate; cultivating in dark place at the temperature of 25-28 DEG C for 70 days; and finally, fruiting indoors or under forest soil. According to the method, the occurrence of Chinese cryptoporus volvatus fruiting body is promoted, and the original basic quality of the Chinese cryptoporus volvatus is ensured. The method has less investment, low cost, stable yield and is easy in management.
Description
Technical field
The invention belongs to biological technical field, particularly, relate to the method for a kind of China hidden pore fungi Bag Material fruiting cultivation.
Background technology
The hidden pore fungi Cryptoporus sinensis popular name Cryptoporus volvatus (peck) Scnear of China, lotus look bacterium.Belong to mycota, Basidiomycotina (Basdiomyeotina), Hymenomycetes (Hymenomyeetes), non-pleat order (Aphyllophorales), Polyporaceae (Polyporaceae), hidden pore fungi genus (Cryptoporus).Mainly be distributed in the ground such as south of Yunnan, the northwestward, South Sichuan and Guangxi of China.The fruit body mildly bitter flavor, property is flat, has cough-relieving, relievings asthma, the function such as detoxifcation, among the peoplely is mainly used in controlling tracheitis, asthma and children's's wean.For a long time, people rely on the wild resource to keep to the utilization of the hidden pore fungi of China always, so market demand is very large, cause Chinese hidden pore fungi wild resource fewer and feweri.Therefore, seek cultivation method extremely urgent.
Summary of the invention
The object of the present invention is to provide a kind of method for cultivating Chinese cryptoporus volvatus, adopt the method to carry out Chinese hidden pore fungi bag in indoor or sylvan life and plant, less investment, cost is low, and stable yield is easy to management.
Above-mentioned purpose of the present invention is to realize by following technical scheme:
A kind of method for cultivating Chinese cryptoporus volvatus is got 25 ℃ of Chinese cryptoporus volvatus kinds of solid of cultivating 7 days, adopts in the bacterium bag that cultivation bacterium material is housed after the plum blossom-shaped multiple spot is inoculated in sterilization, and 25-28 ℃ of lucifuge cultivated after 70 days, carried out fruiting in indoor or sylvan life.
Described method for cultivating Chinese cryptoporus volvatus, wherein cultivating the bacterium material formula is needle wood chip 40-50%, cotton seed hulls 25-35%, wheat bran 15-20%, analysis dusty yeast 0.5-1%, rosin 0.5-1%, gypsum 1-2%, superphosphate 0.5-1%.
Described method for cultivating Chinese cryptoporus volvatus is that above-mentioned batching is stirred, and adding water to its water content is 50-60%, and in the cultivation bag of packing into, sterilization 2 hours or 100 ℃ of sterilizations are 15-20 hour under 121-126 ℃.
Described method for cultivating Chinese cryptoporus volvatus, wherein inoculation is when the good cultivation bacterium bag temperature of sterilization drops to below 30 ℃, adopts the inoculation of plum blossom-shaped multiple spot.
Described method for cultivating Chinese cryptoporus volvatus, wherein cultivating is that postvaccinal bacterium bag is stacked 5 layers by well shape or wall formula, 25-28 ℃ of lucifuge cultivated 60-70 days.
Described method for cultivating Chinese cryptoporus volvatus, wherein fruiting is that cultured bacterium bag is moved into mushroom room or the sylvan life that disinfects, and keeps air humidity at 50-90%, temperature 28-30 ℃, intensity of illumination 200-500 lux, light application time 8-10 hour/day.
More specifically, technical scheme of the present invention can be described as:
1, Chinese cryptoporus volvatus kind is prepared: adopt conventional edible fungus species making, breeding method to make Chinese hidden pore fungi cultivated species, this cultivated species is the 20-25 ℃ of solid spawn of cultivating 5-10 days.
2, planting material proportioning: be needle wood chip 40-50%, cotton seed hulls 25-35%, wheat bran 15-20%, analysis dusty yeast 0.5-1%, rosin 0.5-1%, gypsum 1-2%, superphosphate 0.5-1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 50-60%, the Polypropylene Bag of packing into, and 121-126 ℃ of sterilization sterilized 15-20 hour in 2 hours or 100 ℃.
4, inoculation: when bacterium bag temperature drops to 30 ℃, just can adopt the inoculation of plum blossom fado point at transfer room, inoculate the 6-10 hole for every bag.
5, cultivate: postvaccinal bacterium bag is pressed well shape or wall formula stacking 5-8 layer, and 25-28 ℃ of lucifuge cultivated 60-70 days.
6, management of producing mushroom: cultured bacterium bag is moved into the mushroom room that disinfects, keep air humidity at 50-90%, temperature 25-30 ℃, intensity of illumination is 200-500 lux (LX scattered light), light application time 8-10 hour/day, promote Chinese hidden pore fungi fruit body to occur.
Adopt method of the present invention to carry out Chinese hidden pore fungi bag in indoor or sylvan life and plant, the one, adding rosin is to guarantee that the active ingredient of cultivated species and wild species is substantially suitable, has medical value; The 2nd, adding dusty yeast is to satisfy it to the demand of protein, promotes mycelial growth, and improves the mycelia vigor, has realized that finally biological transformation ratio is at 20-30%.This method less investment, cost is low, and stable yield is easy to management.
Embodiment:
Embodiment 1:
1, Chinese cryptoporus volvatus kind is prepared: get 25 ℃ of Chinese cryptoporus volvatus kinds of solid of cultivating 7 days.
2, planting material proportioning: needle wood chip 48%, cotton seed hulls 30%, wheat bran 18%, analysis dusty yeast 1%, rosin 1%, gypsum 1%, superphosphate 1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 55%, and the Polypropylene Bag of packing into was sterilized 2 hours for 125 ℃.
4, inoculation: when the bacterium bag temperature of the 3rd step drops to 30 ℃, just can adopt 1 ready bacterial classification of plum blossom fado point inoculation step at transfer room, inoculate 6 holes for every bag.
5, cultivate: postvaccinal bacterium bag press well shape or the wall formula is stacked 5 layers, 28 ℃ of lucifuges cultivations 70 days.
6, management of producing mushroom: cultured bacterium bag is moved into the mushroom room that disinfects, and keeps air humidity 80%, and 28 ℃ of temperature, intensity of illumination are 500 luxs (LX scattered lights), light application time 8 hours/day.Just can gather after fruit body was launched in 15 days.
Embodiment 2:
1, Chinese cryptoporus volvatus kind is prepared: adopt 25 ℃ of Chinese cryptoporus volvatus kinds of solid of cultivating 7 days.
2, planting material proportioning: needle wood chip 43%, cotton seed hulls 35%, wheat bran 18%, analysis dusty yeast 1%, rosin 1%, gypsum 1%, superphosphate 1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 50%, and the Polypropylene Bag of packing into was sterilized 20 hours for 100 ℃.
4, inoculation: when bacterium bag temperature drops to 30 ℃, just can adopt the inoculation of plum blossom fado point at transfer room, inoculate 10 holes for every bag.
5, cultivate: postvaccinal bacterium bag press well shape or the wall formula is stacked 8 layers, 28 ℃ of lucifuges cultivations 60 days.
6, management of producing mushroom: cultured bacterium bag is moved into the mushroom room that disinfects, and keeps air humidity 50%, 25 ℃ of temperature, and keeping intensity of illumination is 400 luxs (LX scattered lights), light application time 9 hours/day.Just can gather after fruit body was launched in 18 days.
Embodiment 3:
1, Chinese cryptoporus volvatus kind is prepared: adopt 25 ℃ of Chinese cryptoporus volvatus kinds of solid of cultivating 7 days.
2, planting material proportioning: needle wood chip 42%, cotton seed hulls 35%, wheat bran 19%, analysis dusty yeast 0.5%, rosin 0.5%, gypsum 2%, superphosphate 1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 60%, and the Polypropylene Bag of packing into was sterilized 20 hours for 100 ℃.
4, inoculation: when bacterium bag temperature drops to 30 ℃, just can adopt the inoculation of plum blossom fado point at transfer room, inoculate 10 holes for every bag.
5, cultivate: postvaccinal bacterium bag press well shape or the wall formula is stacked 8 layers, 28 ℃ of lucifuges cultivations 60 days.
6, management of producing mushroom: cultured bacterium bag is moved into the mushroom room that disinfects, and keeps air humidity 50%, 26 ℃ of temperature, and keeping intensity of illumination is 300 luxs (LX scattered lights), light application time 10 hours/day.Just can gather after fruit body was launched in 20 days.
Embodiment 4:
1, Chinese cryptoporus volvatus kind is prepared: adopt 25 ℃ of Chinese cryptoporus volvatus kinds of solid of cultivating 7 days.
2, planting material proportioning: needle wood chip 40%, cotton seed hulls 35%, wheat bran 20%, analysis dusty yeast 1%, rosin 1%, gypsum 2%, superphosphate 1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 58%, and the Polypropylene Bag of packing into was sterilized 20 hours for 100 ℃.
4, inoculation: when bacterium bag temperature drops to 30 ℃, just can adopt the inoculation of plum blossom fado point at transfer room, inoculate 10 holes for every bag.
5, cultivate: postvaccinal bacterium bag press well shape or the wall formula is stacked 8 layers, 28 ℃ of lucifuges cultivations 60 days.
6, management of producing mushroom: cultured bacterium bag is moved into the mushroom room that disinfects, and keeps air humidity 50%, 27 ℃ of temperature, and keeping intensity of illumination is 200 luxs (LX scattered lights), light application time 10 hours/day.Just can gather after fruit body was launched in 20 days.
Claims (1)
1. method for cultivating Chinese cryptoporus volvatus, get 25 ℃ of Chinese hidden pore fungi original seeds of cultivating 7 days, adopt the plum blossom-shaped multiple spot to be inoculated in high pressure 121-126 ℃ of sterilization 2 hours or 100 ℃ of sterilizations of normal pressure and after 15-20 hour 40-50% needle wood chip is housed, the 25-35% cotton seed hulls, 15-20% wheat bran, 0.5-1% analyzes and uses dusty yeast, 0.5-1% rosin, the 1-2% gypsum, 0.5-1% superphosphate, water content is in the bacterium bag of 50-60%, postvaccinal bacterium bag is pressed groined type or wall formula stack 5 layers, 25-28 ℃ of lucifuge cultivated after 70 days, move into the mushroom room or the sylvan life that disinfect, keep air humidity at 50-90%, temperature 28-30 ℃, intensity of illumination 200-500 lux, carried out fruiting in light application time 8-10 hour/day.
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| CN105165411B (en) * | 2015-11-03 | 2018-08-10 | 哈密市庆丰设施农业科技开发有限公司 | The planting technique of wild orange mushroom |
| CN108070529A (en) * | 2018-01-18 | 2018-05-25 | 杭州科格生物科技有限公司 | A kind of production method of solid state rheology cryptoporus volvatus powder and its application |
| CN111919664A (en) * | 2020-08-11 | 2020-11-13 | 南京康之春生物科技有限公司 | Pholiota nameko culture medium and pholiota nameko culture method |
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| EP1422292A1 (en) * | 2001-08-17 | 2004-05-26 | Hangzhou Tsbio Science & Technology Co., Ltd. | Fermentation product of cyptoporus volvatus and its preparation method and use |
| CN101803531A (en) * | 2010-03-26 | 2010-08-18 | 中国科学院微生物研究所 | China cryptoporus sinensis and solid cultural method and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1422292A1 (en) * | 2001-08-17 | 2004-05-26 | Hangzhou Tsbio Science & Technology Co., Ltd. | Fermentation product of cyptoporus volvatus and its preparation method and use |
| CN101803531A (en) * | 2010-03-26 | 2010-08-18 | 中国科学院微生物研究所 | China cryptoporus sinensis and solid cultural method and application thereof |
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| 吴锦忠等.隐孔菌的发酵培养及成分分析.《福建中医学院学报》.1999,(第01期), |
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