CN102498948A - Method for cultivating Chinese cryptoporus volvatus - Google Patents

Method for cultivating Chinese cryptoporus volvatus Download PDF

Info

Publication number
CN102498948A
CN102498948A CN2011103483683A CN201110348368A CN102498948A CN 102498948 A CN102498948 A CN 102498948A CN 2011103483683 A CN2011103483683 A CN 2011103483683A CN 201110348368 A CN201110348368 A CN 201110348368A CN 102498948 A CN102498948 A CN 102498948A
Authority
CN
China
Prior art keywords
china
percent
days
pore fungi
cultivation method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN2011103483683A
Other languages
Chinese (zh)
Other versions
CN102498948B (en
Inventor
赵琪
杨祝良
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kunming Institute of Botany of CAS
Original Assignee
Kunming Institute of Botany of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kunming Institute of Botany of CAS filed Critical Kunming Institute of Botany of CAS
Priority to CN 201110348368 priority Critical patent/CN102498948B/en
Publication of CN102498948A publication Critical patent/CN102498948A/en
Application granted granted Critical
Publication of CN102498948B publication Critical patent/CN102498948B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a method for cultivating Chinese cryptoporus volvatus, comprising the following steps of: taking solid Chinese cryptoporus volvatus microorganism, which is cultivated at the temperature of 25 DEG C for 7 days, to be plum blossom-shaped multi-point inoculated to a fungus cultivation bag with cultivation substrates comprising 40-50 percent of needle leave sawdust, 25-35 percent of cottonseed hull, 15-20 percent of wheat bran, 0.5-1 percent of yeast powder for analysis, 0.5-1 percent of turpentine, 1-2 percent of gypsum, and 0.5-1 percent of superphosphate; cultivating in dark place at the temperature of 25-28 DEG C for 70 days; and finally, fruiting indoors or under forest soil. According to the method, the occurrence of Chinese cryptoporus volvatus fruiting body is promoted, and the original basic quality of the Chinese cryptoporus volvatus is ensured. The method has less investment, low cost, stable yield and is easy in management.

Description

A kind of China conceals the pore fungi cultivation method
Technical field
The invention belongs to biological technical field, particularly, relate to the method for the latent pore fungi Bag Material fruiting cultivation of a kind of China.
Background technology
The latent pore fungi Cryptoporus sinensis popular name Cryptoporus volvatus (peck) Scnear of China, lotus look bacterium.Belong to mycota, Basidiomycotina (Basdiomyeotina), Hymenomycetes (Hymenomyeetes), non-pleat order (Aphyllophorales), Polyporaceae (Polyporaceae), latent pore fungi genus (Cryptoporus).Mainly be distributed in the ground such as south of Yunnan, the northwestward, South Sichuan and Guangxi of China.The fruit body mildly bitter flavor, property is flat, has cough-relieving, relievings asthma, function such as detoxifcation, and among the people being mainly used in controlled tracheitis, asthma and children's's wean.For a long time, people rely on natural wild resource to keep to the utilization of the latent pore fungi of China always, so market demand is very big, cause the latent pore fungi wild resource of China fewer and feweri.Therefore, it is extremely urgent to seek cultivation method.
Summary of the invention
The object of the present invention is to provide a kind of China to conceal the pore fungi cultivation method, adopt this method to carry out the latent pore fungi bag of China and plant in indoor or sylvan life, less investment, cost is low, and stable yield is easy to management.
Above-mentioned purpose of the present invention is to realize through following technical scheme:
A kind of China conceals the pore fungi cultivation method, gets 25 ℃ of solid China cryptoporus volvatus kinds of cultivating 7 days, adopts in the bacterium bag that the culture bacteria material is housed after plum blossom shape multiple spot is inoculated in sterilization, and 25-28 ℃ of lucifuge cultivated after 70 days, carried out fruiting in indoor or sylvan life.
Described China conceals the pore fungi cultivation method, and wherein the culture bacteria material formula is needle wood chip 40-50%, cotton seed hulls 25-35%, wheat bran 15-20%, analyzes with dusty yeast 0.5-1%, rosin 0.5-1%, gypsum 1-2%, superphosphate 0.5-1%.
Described China conceals the pore fungi cultivation method, is that above-mentioned batching is stirred, and adding water to its water content is 50-60%, in the cultivation bag of packing into, sterilizes down 2 hours or 100 ℃ for 121-126 ℃ and sterilizes 15-20 hour.
Described China conceals the pore fungi cultivation method, and wherein inoculation is when the good culture bacteria bag temperature of sterilization drops to below 30 ℃, adopts the inoculation of plum blossom shape multiple spot.
Described China conceals the pore fungi cultivation method, and wherein cultivating is that postvaccinal bacterium bag is stacked 5 layers by well shape or wall formula, and 25-28 ℃ of lucifuge cultivated 60-70 days.
Described China conceals the pore fungi cultivation method, and wherein fruiting is that cultured bacterium bag is moved into fruiting room or the sylvan life that disinfects, and keeps air humidity at 50-90%, and temperature 28-30 ℃, intensity of illumination 200-500 lux, light application time 8-10 hour/day.
More specifically, technical scheme of the present invention can be described as:
1, Chinese cryptoporus volvatus kind is prepared: adopt conventional edible fungus species making, breeding method to make the latent pore fungi cultivated species of China, this cultivated species is a 20-25 ℃ of solid spawn of cultivating 5-10 days.
2, cultivate material proportioning: for needle wood chip 40-50%, cotton seed hulls 25-35%, wheat bran 15-20%, analyze with dusty yeast 0.5-1%, rosin 0.5-1%, gypsum 1-2%, superphosphate 0.5-1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 50-60%, the Polypropylene Bag of packing into, and 121-126 ℃ of sterilization sterilized 15-20 hour in 2 hours or 100 ℃.
4, inoculation: when bacterium bag temperature drops to 30 ℃, just can adopt the inoculation of plum blossom fado point, every bag of inoculation 6-10 hole at transfer room.
5, cultivate: postvaccinal bacterium bag is pressed well shape or wall formula stacking 5-8 layer, and 25-28 ℃ of lucifuge cultivated 60-70 days.
6, management of producing mushroom: cultured bacterium bag is moved into the fruiting room that disinfects, and keeps air humidity at 50-90%, and temperature 25-30 ℃, intensity of illumination is 200-500 lux (a LX scattered light), light application time 8-10 hour/day, promotes the latent pore fungi fruit body of China to take place.
Adopt method of the present invention to carry out the latent pore fungi bag of China and plant in indoor or sylvan life, the one, adding rosin is to guarantee that the active ingredient of cultivated species and wild species is suitable basically, has medical value; The 2nd, adding dusty yeast is to satisfy its demand to protein, promotes mycelial growth, and improves the mycelia vigor, has realized that finally biological transformation ratio is at 20-30%.This method less investment, cost is low, and stable yield is easy to management.
Embodiment:
Embodiment 1:
1, Chinese cryptoporus volvatus kind is prepared: get 25 ℃ of solid China cryptoporus volvatus kinds of cultivating 7 days.
2, cultivate material proportioning: needle wood chip 48%, cotton seed hulls 30%, wheat bran 18%, analysis are with dusty yeast 1%, rosin 1%, gypsum 1%, superphosphate 1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 55%, and the Polypropylene Bag of packing into was sterilized 2 hours for 125 ℃.
4, inoculation: when the bacterium bag temperature of the 3rd step drops to 30 ℃, just can adopt 1 ready bacterial classification of plum blossom fado point inoculation step, inoculate 6 holes for every bag at transfer room.
5, cultivate: postvaccinal bacterium bag press well shape or the wall formula is stacked 5 layers, 28 ℃ of lucifuges cultivations 70 days.
6, management of producing mushroom: cultured bacterium bag is moved into the fruiting room that disinfects, and keeps air humidity 80%, and 28 ℃ of temperature, intensity of illumination are 500 luxs (LX scattered lights), light application time 8 hours/day.15 days fruit bodys are launched the back and just can be gathered.
Embodiment 2:
1, Chinese cryptoporus volvatus kind is prepared: adopt 25 ℃ of solid China cryptoporus volvatus kinds of cultivating 7 days.
2, cultivate material proportioning: needle wood chip 43%, cotton seed hulls 35%, wheat bran 18%, analysis are with dusty yeast 1%, rosin 1%, gypsum 1%, superphosphate 1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 50%, and the Polypropylene Bag of packing into was sterilized 20 hours for 100 ℃.
4, inoculation: when bacterium bag temperature drops to 30 ℃, just can adopt the inoculation of plum blossom fado point, every bag of inoculation 10 holes at transfer room.
5, cultivate: postvaccinal bacterium bag press well shape or the wall formula is stacked 8 layers, 28 ℃ of lucifuges cultivations 60 days.
6, management of producing mushroom: cultured bacterium bag is moved into the fruiting room that disinfects, and keeps air humidity 50%, 25 ℃ of temperature, and keeping intensity of illumination is 400 luxs (LX scattered lights), light application time 9 hours/day.18 days fruit bodys are launched the back and just can be gathered.
Embodiment 3:
1, Chinese cryptoporus volvatus kind is prepared: adopt 25 ℃ of solid China cryptoporus volvatus kinds of cultivating 7 days.
2, cultivate material proportioning: needle wood chip 42%, cotton seed hulls 35%, wheat bran 19%, analysis are with dusty yeast 0.5%, rosin 0.5%, gypsum 2%, superphosphate 1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 60%, and the Polypropylene Bag of packing into was sterilized 20 hours for 100 ℃.
4, inoculation: when bacterium bag temperature drops to 30 ℃, just can adopt the inoculation of plum blossom fado point, every bag of inoculation 10 holes at transfer room.
5, cultivate: postvaccinal bacterium bag press well shape or the wall formula is stacked 8 layers, 28 ℃ of lucifuges cultivations 60 days.
6, management of producing mushroom: cultured bacterium bag is moved into the fruiting room that disinfects, and keeps air humidity 50%, 26 ℃ of temperature, and keeping intensity of illumination is 300 luxs (LX scattered lights), light application time 10 hours/day.20 days fruit bodys are launched the back and just can be gathered.
Embodiment 4:
1, Chinese cryptoporus volvatus kind is prepared: adopt 25 ℃ of solid China cryptoporus volvatus kinds of cultivating 7 days.
2, cultivate material proportioning: needle wood chip 40%, cotton seed hulls 35%, wheat bran 20%, analysis are with dusty yeast 1%, rosin 1%, gypsum 2%, superphosphate 1%.
3, pack, sterilization: above-mentioned batching is stirred, and adding water to its water content is 58%, and the Polypropylene Bag of packing into was sterilized 20 hours for 100 ℃.
4, inoculation: when bacterium bag temperature drops to 30 ℃, just can adopt the inoculation of plum blossom fado point, every bag of inoculation 10 holes at transfer room.
5, cultivate: postvaccinal bacterium bag press well shape or the wall formula is stacked 8 layers, 28 ℃ of lucifuges cultivations 60 days.
6, management of producing mushroom: cultured bacterium bag is moved into the fruiting room that disinfects, and keeps air humidity 50%, 27 ℃ of temperature, and keeping intensity of illumination is 200 luxs (LX scattered lights), light application time 10 hours/day.20 days fruit bodys are launched the back and just can be gathered.

Claims (6)

1. the latent pore fungi cultivation method of China is got 25 ℃ of solid China cryptoporus volvatus kinds of cultivating 7 days, adopts in the bacterium bag that the culture bacteria material is housed after plum blossom shape multiple spot is inoculated in sterilization, and 25-28 ℃ of lucifuge cultivated after 70 days, carried out fruiting in indoor or sylvan life.
2. China as claimed in claim 1 conceals the pore fungi cultivation method, it is characterized in that described culture bacteria material formula is needle wood chip 40-50%, cotton seed hulls 25-35%, wheat bran 15-20%, analyzes with dusty yeast 0.5-1%, rosin 0.5-1%, gypsum 1-2%, superphosphate 0.5-1%.
3. China as claimed in claim 1 conceals the pore fungi cultivation method; It is characterized in that with the culture bacteria material formula be needle wood chip 40-50%, cotton seed hulls 25-35%, wheat bran 15-20%, the batching analyzed with dusty yeast 0.5-1%, rosin 0.5-1%, gypsum 1-2%, superphosphate 0.5-1% stirs; Adding water to its water content is 50-60%; Pack in the cultivation bag, sterilized down 2 hours or 100 ℃ for 121-126 ℃ and sterilized 15-20 hour.
4. China as claimed in claim 1 conceals the pore fungi cultivation method, it is characterized in that described inoculation is when the good bacterium bag temperature of sterilization drops to below 30 ℃, adopts the inoculation of plum blossom shape multiple spot.
5. China as claimed in claim 1 conceals the pore fungi cultivation method, it is characterized in that described cultivation is that the postvaccinal bacterium bag that the culture bacteria material is housed is stacked 5 layers by well shape or wall formula, and 25-28 ℃ of lucifuge cultivated 60-70 days.
6. China as claimed in claim 1 conceals the pore fungi cultivation method; It is characterized in that described fruiting is that cultured bacterium bag is moved into fruiting room or the sylvan life that disinfects, keep air humidity, temperature 28-30 ℃ at 50-90%; Intensity of illumination 200-500 lux, light application time 8-10 hour/day.
CN 201110348368 2011-11-07 2011-11-07 Method for cultivating Chinese cryptoporus volvatus Active CN102498948B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201110348368 CN102498948B (en) 2011-11-07 2011-11-07 Method for cultivating Chinese cryptoporus volvatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201110348368 CN102498948B (en) 2011-11-07 2011-11-07 Method for cultivating Chinese cryptoporus volvatus

Publications (2)

Publication Number Publication Date
CN102498948A true CN102498948A (en) 2012-06-20
CN102498948B CN102498948B (en) 2013-06-19

Family

ID=46211130

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201110348368 Active CN102498948B (en) 2011-11-07 2011-11-07 Method for cultivating Chinese cryptoporus volvatus

Country Status (1)

Country Link
CN (1) CN102498948B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105165411A (en) * 2015-11-03 2015-12-23 哈密市庆丰设施农业科技开发有限公司 Cultivation technique for wild orange mushroom
CN108070529A (en) * 2018-01-18 2018-05-25 杭州科格生物科技有限公司 A kind of production method of solid state rheology cryptoporus volvatus powder and its application
CN111919664A (en) * 2020-08-11 2020-11-13 南京康之春生物科技有限公司 Pholiota nameko culture medium and pholiota nameko culture method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1422292A1 (en) * 2001-08-17 2004-05-26 Hangzhou Tsbio Science & Technology Co., Ltd. Fermentation product of cyptoporus volvatus and its preparation method and use
CN101803531A (en) * 2010-03-26 2010-08-18 中国科学院微生物研究所 China cryptoporus sinensis and solid cultural method and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1422292A1 (en) * 2001-08-17 2004-05-26 Hangzhou Tsbio Science & Technology Co., Ltd. Fermentation product of cyptoporus volvatus and its preparation method and use
CN101803531A (en) * 2010-03-26 2010-08-18 中国科学院微生物研究所 China cryptoporus sinensis and solid cultural method and application thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
兰进等: "《药用真菌栽培实用技术》", 30 June 2001, 中国农业出版社 *
华启洪等: "药用真菌隐孔菌的生物学特性研究", 《中国中药杂志》 *
吴锦忠等: "隐孔菌的发酵培养及成分分析", 《福建中医学院学报》 *
屠六邦等: "隐孔菌人工栽培研究", 《吉林农业大学学报》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105165411A (en) * 2015-11-03 2015-12-23 哈密市庆丰设施农业科技开发有限公司 Cultivation technique for wild orange mushroom
CN105165411B (en) * 2015-11-03 2018-08-10 哈密市庆丰设施农业科技开发有限公司 The planting technique of wild orange mushroom
CN108070529A (en) * 2018-01-18 2018-05-25 杭州科格生物科技有限公司 A kind of production method of solid state rheology cryptoporus volvatus powder and its application
CN111919664A (en) * 2020-08-11 2020-11-13 南京康之春生物科技有限公司 Pholiota nameko culture medium and pholiota nameko culture method

Also Published As

Publication number Publication date
CN102498948B (en) 2013-06-19

Similar Documents

Publication Publication Date Title
CN103820348B (en) The production method of one strain plant growth-promoting bacteria and microbial inoculum thereof and application
CN106818215B (en) Industrialized cultivation method of morchella esculenta
CN106396807A (en) Culture medium for improving bioconversion rate of edible fungi, preparation method of culture medium and cultivation method of edible fungi
CN106034742B (en) A method of clitocybe maxima is produced using mulberry bar, bagasse and silkworm excrement
CN103382134A (en) Fermentation method of Agaricus bisporus (Lange) Sing cultivation matrix
CN103382139A (en) Agaricus bisporus (Lange) Sing culture medium
CN103583225A (en) Method for cultivating good-quality high-yield pleurotus geesteranus by means of cassava stems
CN106613349A (en) Tremella aurantialba substitute cultivation method
CN101836597A (en) New grey white variant strain with red pleurotus
CN102273377A (en) Method for cultivating boletus or red fungus
CN109042063A (en) A kind of culture medium for cultivating, preparation method and a kind of Phlebopus portentosus batch production bacterium bag cultural method
CN104609943A (en) Novel medium for cultivating pleurotus ostreatus and method for cultivating pleurotus ostreatus by using novel medium
CN104987151A (en) Cultivation medium for pleurotus eryngii Quel and cultivation method of pleurotus eryngii Quel
CN110679389B (en) Lepista nuda planting method
CN109673389A (en) A kind of edible fungi substrate synergist and preparation method thereof
CN101601412A (en) The purposes of bacillus subtilis and the production method of this bacterial strain
CN103044126A (en) Lentinus edodes cultivation material and making method thereof
CN104303833A (en) Method for cultivating Taiwan pleurotus geesteranus in north of Anhui province
CN101480138B (en) Method for cultivating Agaricus bisporus using wormcast as adding material of cultivation material
CN103229664A (en) Method for planting sweet lucid ganoderma by usage of uncrushed tea seed shells
CN104982178A (en) Method for interplanting panus giganteus in sugarcane field
CN108770592B (en) Culture medium for pholiota citrina culture and pholiota citrina culture method
CN102487725A (en) Method for culturing hypsizygus marmoreus by using corn byproduct
CN102498948B (en) Method for cultivating Chinese cryptoporus volvatus
CN106831082A (en) Improve the planting material and its cultural method of clitocybe maxima yield and amino acid content

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant