CN101601412A - The purposes of bacillus subtilis and the production method of this bacterial strain - Google Patents
The purposes of bacillus subtilis and the production method of this bacterial strain Download PDFInfo
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Abstract
The invention discloses the purposes of a kind of bacillus subtilis and the production method of this bacterial strain, with corn flour 10～25g, dregs of beans 10～35g, honey 3～5g, sodium carbonate 1～2g, dipotassium hydrogen phosphate 1～2g, glycine 1～2g, calcium carbonate 0.5～1.5g, magnesium sulfate 0.2～0.3g, manganese sulphate 0.05～0.15g, iron chloride 0.05～0.15g, the proportioning of sodium chloride 0.3～0.8g and 1000mL water is made into emulsion, insert in the fermentation tank behind 5～8% access Bacillus subtilis strains with the emulsion volume, cultivating 45～60h down 28～30 ℃ of temperature gets final product, the invention provides a kind of Xinjiang special geographic climate of being suitable for, particularly can control biocontrol bacterial strain that the soil-borne disease based on the economic crops of processing tomato and cotton takes place and the process that is suitable for industrial-scale production thereof well, the new biopesticide that is suitable for the use special geographic climate in Xinjiang under for the exploitation condition that provides the foundation.
Description
Technical field:
The present invention relates to a kind of control based on the purposes of the biocontrol bacterial strain of the soil-borne disease generation of the economic crops of processing tomato and cotton and the process that is suitable for industrial-scale production of this biocontrol bacterial strain, the purposes of particularly a kind of bacillus subtilis (Bacillus subtilis) and the production method of this bacterial strain.
Background technology:
At present, Xinjiang unique geography, weather conditions are very suitable for cotton, processing tomato plantation, and plantation of cotton and processing industry are the mainstays of Xinjiang economic development.The processing tomato produced of Xinjiang is because haematochrome, oryzanol content height in addition, low mold count, and viscosity is good, is the first-class raw material of processing tomato goods, becomes topmost processing tomato plantation and processing base.Simultaneously, 1,000,000 mu of bergamot pears are planted in Xinjiang every year, 550,000 mu of grape about 1,500,000 mu and muskmelons.The important pillar of the economy that name, excellent, special melons and fruits such as Kuerle delicious pear, Hami melon and grape are enjoyed great prestige at home and abroad and become vast agro-industrial increasing both production and income, shaken off poverty and set out on the road to prosperity.
But owing to plant year after year, soil-borne disease of crop and pest species are also more and more, generation is more and more heavier in recent years, not only have a strong impact on the output of these important economic crops, also can influence its quality, cause the tremendous economic loss to wide farmers.As 2006～2008 years continuous 3 years because the big generation of the rainy feasible cotton seedling root rot of low temperature in spring, cause a lot of fields piece rot seed, the bud that rots, even large-area situation of ruining kind of replay occurs; Cotton is withered, verticillium wilt morbidity field piece has accounted for 40～50% of sown areas of cotton, and the grave illness field reaches about 20%, and the annual because total crop failure area that cotton is withered, verticillium wilt causes of full boundary is more than 100,000 mu; The processing tomato root rot field incidence of disease reaches 30～50%, and 20～30% plant death can be caused in the grave illness field.And also often cause the death of a large amount of plant by the root rot of the mould and rotten mould grape that causes of epidemic disease, bergamot pear tree.
Therefore the soil-borne disease of successfully controlling on cotton, processing tomato and other, special, the excellent vegetable melon and fruit is to build pressing for of Xinjiang best buy cotton and high-quality industry of planting forest or fruit tress base.
For a long time, chemical pesticide has played huge impetus to the development of the extermination of disease and insect pest and agricultural production.But because chemical pesticide does not add the abuse of restraining, the ecotope that the mankind depend on for existence has suffered serious destruction, environmental pollution constantly increases the weight of, the person poultry poisoning is frequently taken place, and the long-term use of chemical pesticide also causes insect and germ pesticide resistance to strengthen, and is difficult to control the harm of disease worm.
Therefore, many countries will to ecological safety, with the important development direction of the strong biopesticide of Environmental compatibility as the extermination of disease and insect pest.
China also is decided to be fundamental state policy to environmental protection, classifies biopesticide as 21 century and first develops the field.Particularly along with the improving constantly of China joined WTO and living standards of the people, the production of green agricultural product is had higher requirement, the cry of development biopesticide is more surging.
Though the development of China biopesticide is comparatively rapid, but exist the kind of industrialization few, outstanding problem that industrialization level is on the low side, the biopesticide of especially preventing and treating many soil-borne disease of crop is few especially.
Thereby biopesticide can produce certain inhibitory action and play disease-proof functions to these pathogens in the soil, thereby, a large amount of microorganisms that exist in the soil influence its diseases prevention usefulness but also influencing the survival of the main component active microorganism in the biopesticide, there is very strong region adaptability in the microbial pesticide of control soil-borne disease of crop, therefore many microbial pesticides that have fine diseases prevention usefulness in other place have arrived Xinjiang and have many times showed instability.
For example, processing tomato root rot in seedling stage, root rot and cotton seedling root rot, the cotton pathogen withered, verticillium wilt of growth middle and later periods mostly are soil inhabitant, can various states survive in the soil midium or long term, are difficult to from soil its removing.
This efficient disease-preventing microbial that just presses for the suitable native country that utilization filters out from Xinjiang is carried out the industrialization of biopesticide; this is the great demand of Xinjiang agricultural and sustainable economic development and ecological environmental protection, also is the major issue that needs to be resolved hurrily in the microbial pesticide exploitation of Xinjiang.
Although bred many special beneficial microbe monoids under the Special geography climatic environment condition of Xinjiang.But, worked in the past and be limited to tracking more in Xinjiang, basic research is still relatively weaker, causes innovation ability and research and development to lack of staying power thus, and the integral level of microbial pesticide exploitation is compared bigger gap in addition with development speed with developed country and the stronger unit of interior ground scientific research strength.So in the agricultural production process in Xinjiang, chemical pesticide still is in irreplaceable status, thereby and since the abuse of chemical pesticide causes pathogen to develop immunity to drugs cause that protection effect descends, the agricultural product residue of pesticide exceed standard and since the thing that other insects outbursts that Irrational Use of Drugs causes are caused disaster occur repeatedly.
Select efficient diseases prevention biocontrol bacterial strain, exploitation is suitable for the new biopesticide of use under the special geographic climate in Xinjiang, not only can control generation well based on the soil-borne disease on the economic crops of processing tomato and cotton, also Xinjiang agricultural and sustainable economic development can be guaranteed, good economy and ecological benefits will be obtained simultaneously.
The production of new biopesticide needs a large amount of bacterial strains as the basis, therefore, a kind of be suitable for the special geographic climate in Xinjiang, particularly can control the process that is suitable for industrial-scale production of biocontrol bacterial strain that the soil-borne disease based on the economic crops of processing tomato and cotton takes place and this biocontrol bacterial strain well with regard to Ying Ersheng.
Summary of the invention:
Purpose of the present invention provide a kind of be suitable for the special geographic climate in Xinjiang, particularly can control the purposes of the process that is suitable for industrial-scale production, particularly a kind of bacillus subtilis of the purposes of the biocontrol bacterial strain that the soil-borne disease based on the economic crops of processing tomato and cotton takes place and this biocontrol bacterial strain and the production method of this bacterial strain well.
Biocontrol bacterial strain of the present invention is a bacillus subtilis, its characteristics are this microbial strains is used as control or prevents and treats the generation of crops soil-borne disease, and particularly control or control are based on the generation of the soil-borne disease of the economic crops of processing tomato, cotton and capsicum.
As further preferred, then be to be used for processing tomato rotten mould root rot, cotton seedling blight or cotton is withered, the control of verticillium wilt or control.
The production method of above-mentioned bacillus subtilis strain is as follows:
Preparation fermentation medium: corn flour 10～25g, dregs of beans 10～35g, honey 3～5g, sodium carbonate 1～2g, dipotassium hydrogen phosphate 1～2g, glycine 1～2g, calcium carbonate 0.5～1.5g, magnesium sulfate 0.2～0.3g, manganese sulphate 0.05～0.15g, iron chloride 0.05～0.15g, sodium chloride 0.3～0.8g;
Be made into emulsion by the weight of above-mentioned raw materials and the proportioning of 1000mL water, with 5～8% of emulsion volume
Inoculum concentration is inserted in the fermentation tank after inserting Bacillus subtilis strain, under 28～30 ℃ of temperature, cultivate 45～60h and get final product, preferably stirs with 180～220r/min rotating speed during described cultivation.
The above-mentioned carbon source that can substitute corn flour is one or more of starch, glucose or sucrose.
The nitrogenous source that can substitute dregs of beans has one or both of yeast extract, peptone.
Through evidence, after adopting zymocyte liquid root irrigation tomato seedling of the present invention 2 times, can significantly increase the height of tomato seedling and the fresh weight and the dry weight of tomato seedling, though S44 does not increase the height of seedling, but also can significantly increase the fresh weight and the dry weight of tomato seedling, the antagonistic bacterium that further specifies screening has certain growth-promoting functions.
In having inoculated the artificial sick garden of rotten mildew fungus, compare mensuration: test with zymocyte liquid of the present invention, metalaxyl and PCNB respectively.Relative control effect to root rot is as follows: zymocyte liquid of the present invention reaches 44.17, and the control efficiency of % metalaxyl and PCNB is respectively 57.72% and 13.16%.Dry weight and fresh weight to each processing are measured, and the result shows that zymocyte liquid of the present invention can effectively increase the trophosome biomass of processing tomato plant, and the fresh weight of metalaxyl and PCNB processing back plant is lower than contrast, and dry weight has increase to a certain degree.The flower and the fruit quantity of growing on the plant is counted, and the result shows that growth-promoting antagonistic bacterium can significantly increase the quantity of the Hua Heguo on the tomato plant, promptly has significant effect of increasing production, and effect of increasing production is better than metalaxyl, and that the poorest is PCNB.
Zymocyte liquid of the present invention is measured grave illness Tanaka, and the rotten mould root rot of processing tomato is had better control efficiency, can significantly improve the planting percent of tomato seedling, and its protection effect reaches 58.07%.
The antibacterial metabolite of biocontrol microorganisms of the present invention is studied:
By tunning of the present invention is carried out ammonium sulfate precipitation, water and ninhydrin reaction show in the process of zymophyte growth of the present invention and have mainly produced protein-based antibacterial material.In the electric heating constant temperature tank 30 ℃, 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃, 90 ℃ respectively of this antibiotic crude protein, are respectively handled 30min and all can be kept activity for 120 ℃ in pressure cooker by in the electric heating constant temperature air dry oven 100 ℃, 110 ℃.Be all can keep necessarily activity under 3.0,4.0,5.0,6.0,7.0,8.0,9.0,10.0 the condition in the pH value, also can keep better activity to organic solvent ethanol, methyl alcohol, ethyl acetate, acetone, acetonitrile, benzinum, chloroform simultaneously.This antibacterial albumen is except having the higher bacteriostatic activity the processing tomato rhizoctonia solani, eggplant sclerotium, tomato early blight bacterium, withered germ of water-melon, tomato wilt bacterium also there are good inhibitory effect, the bacillary fruit spot germ of pathogenetic bacteria watermelon is also had certain fungistatic effect.
Behind fermented liquid processing tomato seeds of the present invention, the tomato seeds electrical conductivity is reduced, protease and amylase activity raise, be significantly improved with degeneration-resistant relevant enzyme peroxidase (POD), polyphenol oxidase (PPO), active the increasing of hepatocuprein (SOD) in the plant corpus, can cause the disintegration of pathogen mycelia simultaneously.
When using biocontrol microorganisms, adopt seed dressing to add to irritate 2 times mode with water droplet, have best seedling protecting effect and disease prevention growth-promoting effect.This bacterium is after using cotton seeds seed dressing and drip irrigation in addition, and, verticillium wilt withered to cotton seedling blight and the cotton of growth middle and later periods also has protection effect preferably, and short preferably living, effect of increasing production is arranged simultaneously.
Evidence, the invention provides a kind of be suitable for the special geographic climate in Xinjiang, particularly can control the biocontrol bacterial strain that the soil-borne disease based on the economic crops of processing tomato and cotton takes place well, and provide the process that is suitable for industrial-scale production of this biocontrol bacterial strain, for exploitation is suitable under the special geographic climate in Xinjiang the new biopesticide that uses, particularly control biopesticide based on the generation of the soil-borne disease on the economic crops of processing tomato and cotton the condition that provides the foundation.
Embodiment:
Embodiment 1:
Preparation fermentation medium: get corn flour 10Kg, dregs of beans 35Kg, honey 3Kg, sodium carbonate 1Kg, dipotassium hydrogen phosphate 1Kg, magnesium sulfate 0.3Kg, calcium carbonate 1Kg, manganese sulphate 0.1Kg, iron chloride 0.1Kg, sodium chloride 0.5Kg, glycine 1Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 5% inoculum concentration of emulsion volume, under 28 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 180r/min cultivates 50h and gets final product.
Embodiment 2:
Preparation fermentation medium: corn flour 25Kg, dregs of beans 15Kg, honey 5Kg, sodium carbonate 1.2Kg, dipotassium hydrogen phosphate 1.5Kg, glycine 1.5Kg, calcium carbonate 1Kg, magnesium sulfate 0.2Kg, manganese sulphate 0.15Kg, iron chloride 0.1Kg, sodium chloride 0.5Kg;
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 8% inoculum concentration of emulsion volume, under 30 ℃ of temperature, cultivation and fermentation 60h gets final product.
Embodiment 3:
Preparation fermentation medium: corn flour 15Kg, dregs of beans 25Kg, honey 4Kg, sodium carbonate 1.5Kg, dipotassium hydrogen phosphate 1.8Kg, glycine 2Kg, calcium carbonate 0.8Kg, magnesium sulfate 0.25Kg, manganese sulphate 0.15Kg, iron chloride 0.15Kg, sodium chloride 0.6Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 6% inoculum concentration of emulsion volume, under 27 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 210r/min cultivates 55h and gets final product.
Embodiment 4:
Preparation fermentation medium: corn flour 20Kg, dregs of beans 30Kg, honey 5Kg, sodium carbonate 1.8Kg, dipotassium hydrogen phosphate 1.5Kg, glycine 1.6Kg, calcium carbonate 0.8Kg, magnesium sulfate 0.28Kg, manganese sulphate 0.12Kg, iron chloride 0.12Kg, sodium chloride 0.8Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 7% inoculum concentration of emulsion volume, under 28 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 190r/min cultivates 48h and gets final product.
Embodiment 5:
Preparation fermentation medium: corn flour 25Kg, dregs of beans 25Kg, honey 3.5Kg, sodium carbonate 1.4Kg, dipotassium hydrogen phosphate 1.2Kg, glycine 1.8Kg, calcium carbonate 0.8Kg, magnesium sulfate 0.26Kg, manganese sulphate 0.10Kg, iron chloride 0.08Kg, sodium chloride 0.6Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 6.5% inoculum concentration of emulsion volume, under 30 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 180r/min cultivates 48h and gets final product.
Embodiment 6:
Preparation fermentation medium: corn flour 12Kg, dregs of beans 12Kg, honey 3Kg, sodium carbonate 1.5Kg, dipotassium hydrogen phosphate 1.8Kg, glycine 1.8Kg, calcium carbonate 1.5Kg, magnesium sulfate 0.23Kg, manganese sulphate 0.12Kg, iron chloride 0.12Kg, sodium chloride 0.4Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 7.5% inoculum concentration of emulsion volume, under 28.5 ℃ of temperature, cultivation and fermentation 60h gets final product.
Embodiment 7:
Preparation fermentation medium: corn flour 20Kg, dregs of beans 30Kg, honey 3.5Kg, sodium carbonate 1.6Kg, dipotassium hydrogen phosphate 1Kg, glycine 2Kg, calcium carbonate 1.5Kg, magnesium sulfate 0.2Kg, manganese sulphate 0.05Kg, iron chloride 0.15Kg, sodium chloride 0.8Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 7.5% inoculum concentration of emulsion volume, under 29.5 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 220r/min cultivates 48h and gets final product.
Embodiment 8:
Preparation fermentation medium: corn flour 22Kg, dregs of beans 28Kg, honey 3.5Kg, sodium carbonate 1.2Kg, dipotassium hydrogen phosphate 1.2Kg, glycine 1.2Kg, calcium carbonate 1.2Kg, magnesium sulfate 0.2Kg, manganese sulphate 0.1Kg, iron chloride 0.1Kg, sodium chloride 0.5Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 5.5% inoculum concentration of emulsion volume, under 30 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 180r/min cultivates 54h and gets final product.
Embodiment 9:
Preparation fermentation medium: corn flour 25Kg, dregs of beans 30Kg, honey 5Kg, sodium carbonate 2Kg, dipotassium hydrogen phosphate 1Kg, glycine 1Kg, calcium carbonate 1.5Kg, magnesium sulfate 0.3Kg, manganese sulphate 0.15Kg, iron chloride 0.13Kg, sodium chloride 0.8Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 6% inoculum concentration of emulsion volume, under 29 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 180r/min cultivates 60h and gets final product.
Embodiment 10:
Preparation fermentation medium: corn flour 15Kg, dregs of beans 15Kg, honey 3Kg, sodium carbonate 1Kg, dipotassium hydrogen phosphate 2Kg, glycine 1.5Kg, calcium carbonate 1.5Kg, magnesium sulfate 0.25Kg, manganese sulphate 0.15Kg, iron chloride 0.15Kg, sodium chloride 0.7Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 7% inoculum concentration of emulsion volume, under 30 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 210r/min cultivates 48h and gets final product.
Embodiment 11:
Preparation fermentation medium: corn starch 10Kg, peptone 25Kg, honey 3Kg, sodium carbonate 1Kg, dipotassium hydrogen phosphate 1Kg, glycine 2Kg, calcium carbonate 0.5Kg, magnesium sulfate 0.2Kg, manganese sulphate 0.15Kg, iron chloride 0.15Kg, sodium chloride 0.4Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 8% inoculum concentration of emulsion volume, under 29 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 180r/min cultivates 45h and gets final product.
Embodiment 12:
Preparation fermentation medium: corn flour 10～25Kg, dregs of beans 10～35Kg, honey 3～5Kg, sodium carbonate 1～2Kg, dipotassium hydrogen phosphate 1～2Kg, glycine 1～2Kg, calcium carbonate 0.5～1.5Kg, magnesium sulfate 0.2～0.3Kg, manganese sulphate 0.05～0.15Kg, iron chloride 0.05～0.15Kg, sodium chloride 0.3～0.8Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, insert in the fermentation tank after inserting Bacillus subtilis strain with 5～8% inoculum concentration of emulsion volume, under 28～30 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 180～220r/min cultivates 45～60h and gets final product.
Embodiment 13:
Preparation fermentation medium: glucose 20Kg, yeast extract 15Kg, honey 4Kg, sodium carbonate 2Kg, dipotassium hydrogen phosphate 2Kg, glycine 2Kg, calcium carbonate 1.5Kg, magnesium sulfate 0.2Kg, manganese sulphate 0.05Kg, iron chloride 0.1Kg, sodium chloride 0.3Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 8% inoculum concentration of emulsion volume, under 30 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 180r/min cultivates 60h and gets final product.
Embodiment 14:
Preparation fermentation medium: sucrose 25Kg, yeast extract 10Kg, dregs of beans 10Kg, honey 5Kg, sodium carbonate 2Kg, dipotassium hydrogen phosphate 1Kg, glycine 1Kg, calcium carbonate 1.5Kg, magnesium sulfate 0.25Kg, manganese sulphate 0.15Kg, iron chloride 0.15Kg, sodium chloride 0.5Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 5～8% inoculum concentration of emulsion volume, under 30 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 220r/min cultivates 48h and gets final product.
Embodiment 15:
Preparation fermentation medium: starch from sweet potato 10Kg, corn flour 10Kg, peptone 10Kg, yeast extract 10Kg dregs of beans 10Kg, honey 5Kg, sodium carbonate 1.5Kg, dipotassium hydrogen phosphate 1.5Kg, glycine 1.5Kg, calcium carbonate 1.5Kg, magnesium sulfate 0.25Kg, manganese sulphate 0.15Kg, iron chloride 0.15Kg, sodium chloride 0.8Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 6% inoculum concentration of emulsion volume, under 30 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 180r/min cultivates 48h and gets final product.
Embodiment 16:
Preparation fermentation medium: sucrose 5Kg, potato starch 5Kg, glucose 5Kg, corn flour 10Kg, yeast extract 10Kg, peptone 10Kg, dregs of beans 15Kg, honey 5Kg, sodium carbonate 2Kg, dipotassium hydrogen phosphate 1Kg, glycine 1Kg, calcium carbonate 1Kg, magnesium sulfate 0.2Kg, manganese sulphate 0.15Kg, iron chloride 0.15Kg, sodium chloride 0.6Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 5% inoculum concentration of emulsion volume, under 30 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 180r/min cultivates 60h and gets final product.
Embodiment 17:
Preparation fermentation medium: sucrose 10Kg, potato starch 10Kg, yeast extract 15Kg, peptone 15Kg, dregs of beans 5Kg, honey 3Kg, sodium carbonate 1.5Kg, dipotassium hydrogen phosphate 1.6Kg, glycine 1.5Kg, calcium carbonate 1.5Kg, magnesium sulfate 0.2Kg, manganese sulphate 0.15Kg, iron chloride 0.15Kg, sodium chloride 0.6Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 8% inoculum concentration of emulsion volume, under 28 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 210r/min cultivates 60h and gets final product.
Embodiment 18:
Preparation fermentation medium: sucrose 5Kg, potato starch 5Kg, glucose 5Kg, corn flour 5Kg, yeast extract 5Kg, peptone 20Kg, dregs of beans 10Kg, honey 4Kg, sodium carbonate 1Kg, dipotassium hydrogen phosphate 2Kg, glycine 1.5Kg, calcium carbonate 1.5Kg, magnesium sulfate 0.12Kg, manganese sulphate 0.1Kg, iron chloride 0.15g, sodium chloride 0.5Kg.
The proportioning of above-mentioned raw materials and 1000L water is made into emulsion, inserts in the fermentation tank after inserting Bacillus subtilis strain with 6% inoculum concentration of emulsion volume, under 28 ℃ of temperature, cultivation and fermentation and stirring, rotating speed 200r/min cultivates 54h and gets final product.
Claims (8)
1, the purposes of a kind of bacillus subtilis is characterized in that the generation of this microbial strains as control or control crops soil-borne disease.
2, the purposes of bacillus subtilis according to claim 1 is characterized in that described crops are processing tomato, cotton.
3, the purposes of bacillus subtilis according to claim 2 is characterized in that described soil-borne disease is processing tomato rotten mould root rot, cotton seedling blight or cotton is withered, verticillium wilt.
4, a kind of production method according to each described bacillus subtilis strain of claim 1～3, its feature production process is as follows:
Preparation fermentation medium: corn flour 10～25g, dregs of beans 10～35g, honey 3～5g, sodium carbonate 1～2g, dipotassium hydrogen phosphate 1～2g, glycine 1～2g, calcium carbonate 0.5～1.5g, magnesium sulfate 0.2～0.3g, manganese sulphate 0.05～0.15g, iron chloride 0.05～0.15g, sodium chloride 0.3～0.8g.
Be made into emulsion by the weight of above-mentioned raw materials and the proportioning of 1000mL water, insert in the fermentation tank after inserting Bacillus subtilis strain with 5～8% inoculum concentration of emulsion volume, cultivate 45～60h down for 28～30 ℃ in temperature.
5, the production method of bacillus subtilis strain according to claim 4 stirs under the rotating speed of 180～220r/min when it is characterized in that cultivating in the described fermentation tank.
6, according to the production method of claim 4 or 5 described bacillus subtilis strains, the carbon source that it is characterized in that described corn flour is replaced by one or more of starch, glucose or sucrose.
7, according to the production method of claim 4 or 5 described bacillus subtilis strains, the nitrogenous source that it is characterized in that described dregs of beans yeast extract, peptone arranged one or both replace.
8, the production method of bacillus subtilis strain according to claim 6, the nitrogenous source that it is characterized in that described dregs of beans yeast extract, peptone arranged one or both replace.
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| CN118308278A (en) * | 2024-06-13 | 2024-07-09 | 珠海鱼来鱼旺生物科技有限公司 | High-density liquid fermentation medium of bacillus subtilis and fermentation method |
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- 2009-07-16 CN CNA2009101133828A patent/CN101601412A/en active Pending
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| CN114940959A (en) * | 2022-06-09 | 2022-08-26 | 河北农业大学 | Application of bacillus subtilis in promoting growth of crops |
| CN118308278A (en) * | 2024-06-13 | 2024-07-09 | 珠海鱼来鱼旺生物科技有限公司 | High-density liquid fermentation medium of bacillus subtilis and fermentation method |
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Application publication date: 20091216 Assignee: Xinjiang Shida Technology Co.,Ltd. Assignor: Shihezi University Contract record no.: 2012650000030 Denomination of invention: Application of bacillus subtilis and production method for strains License type: Exclusive License Record date: 20121012 |
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