CN104450551A - Bacillus subtilis DPPG-26 for preventing and treating damping off and application thereof - Google Patents
Bacillus subtilis DPPG-26 for preventing and treating damping off and application thereof Download PDFInfo
- Publication number
- CN104450551A CN104450551A CN201410386872.6A CN201410386872A CN104450551A CN 104450551 A CN104450551 A CN 104450551A CN 201410386872 A CN201410386872 A CN 201410386872A CN 104450551 A CN104450551 A CN 104450551A
- Authority
- CN
- China
- Prior art keywords
- dppg
- bacterial strain
- bacillus subtilis
- subtilis
- damping
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses bacillus subtilis DPPG-26 for preventing and treating damping off. The preservation number of a strain is CGMCC No. 9393. The bacillus subtilis DPPG-26 is gram-positive bacterium, has broad-spectrum bacteriostatic activity and extremely high adverse resistance and can be multiplied and colonized on the surface of fungous pathogenic mycelium so as to effectively inhibit the growth of germs and control occurrence of diseases. A bacterial strain DPPG-26 original fermentation solution is 213.3 U1 in bacteriostasis titer; ammonium sulfate is used for step-by-step salting out in combination with ion-exchange column chromatography in order to analyze that an antibacterial substance of the bacillus subtilis DPPG-26 is mainly protein. Besides, chitinase can be generated by the bacillus subtilis DPPG-26, so that extension of pathogenic bacteria rhizoctonia solani hypha can be well inhibited, and the occurrence of the damping off of processing tomatoes can be effectively prevented and treated. After being treated by the zymophyte solution of the bacillus subtilis DPPG-26 disclosed by the invention, a plant is respectively increased by 23.81%, 42.57% and 30.38% in height, fresh weight and dry weight, the prevention and treatment efficiency is 58.62%, and the vegetative body biomass of the plant can be effectively increased. The bacillus subtilis DPPG-26 disclosed by the invention is simple in strain culture condition, easy to store, friendly to environment and great in development and application potential.
Description
Technical field
The invention belongs to agricultural biological technical field.More specifically, the present invention relates to subtilis DPPG-26 and application that a strain prevents and treats damping-off.
Background technology
Processing tomato is the main cash crop in Xinjiang.Processing Tomato in Xinjiang cultivated area reaches more than 100 ten thousand mu; the total output value of tomato year accounts for 90% of the whole nation; tomato industry was through the development of 15 years; industrialization, large-scale production are formed; annual export volume accounts for 70% of national market share, has family of tomato-sauce factory more than 40, builds up tomato-sauce machining production line 151; annual working ability has reached 800,000 tons, and process unit ability accounts for 90% of whole nation processing total capacity.But processing tomato seedling blight occurs serious, directly has influence on the sustainable development of Processing Tomato in Xinjiang industry.Therefore, the biological pesticide finding applicable Xinjiang native country soil and weather condition also can use in conjunction with Xinjiang cultivation mode receive more concerns.
Miliary damping-off germ Rhizictonia solani Kuhn is a kind of soil inhabitant, can parasitic section more than 180, thousands of kind of plant, cotton and tomato seedling are unearthed and all can be injured to field planting.The condition of low temperature and high relative humidity is extremely conducive to the generation of this disease, and because Xinjiang in Spring is colder, crop after planting usually cold spell in later spring and rainy weather can occur, and often causes the large generation of seedling blight, causes harm serious.The general sickness rate of cotton seedling root rot caused by Rhizictonia solani Kuhn is 27%-75%, and serious reaches more than 90%, sometimes because seed decay and seedling death is more, causes the disconnected ridge that is short of seedling, greatly affects plant strain growth.The sickness rate of general processing tomato seedling blight is 10-20%, and some time can reach more than 50%, and the serious time even can cause crop to ruin kind of a replay.In recent years, along with enforcement and the long-term continuous cropping of drip irrigation measure, the trend that this sick incidence rises in addition year by year.
The seedling blight caused by dry thread Pyrenomycetes (Rhizictonia solani) is that a class distribution is wide, harm is heavy, the soil-borne disease of difficult control, in disease control, because chemical pesticide exists contaminate environment, easily cause pesticide residue, destroy the problems such as the eubiosis, and selection and popularization resistant variety is owing to will consider the pathotype of pathogenic bacteria complexity, be therefore difficult to obtain very large breakthrough.For this situation, sight is little by little turned to the efficient biocontrol fungicide preventing and treating damping-off from occurring in nature screening and exploitation by people.Genus bacillus attracts tremendous attention as a kind of good research material always.The many biological and ecological methods to prevent plant disease, pests, and erosion effective strains screened both at home and abroad are all the members of this genus.
Current overseas utilization subtilis prevents and treats the disease caused by Rhizoctonia solan, Pvthium spp., Fusarium spp. and Botryadiplodia solani-tuberosi, obtains good effect.The existing 4 bacillus subtilis biocontrol strains of the U.S. obtain Environmental Protection Agency's (EPA) commercialization or limited merchandized handling application license, and they are GB03, MBI600, QST713 and FZB24.GB03 and MB1600 of Gustafson company of the U.S. and the exploitation of MierobioLtd. company, GB03 and MB1600 for dress seed or root execute can prevent and treat Fusarium, Aspergillus, Altrernaria and Rhizoctonia and cause cotton, beans, wheat class and peanut root disease; Bacterial strain FZB24 is used for greenhouse or indoor cultivation sapling and shrub root can prevent and treat the root rot and blight that Fusarium and Rhizoctonia cause; Bacterial strain QST713 can prevent and treat vegetables, cherry, grape, the bacterium of cucurbit and English walnut and fungal disease.The Bacillus subtills RB14 of Tokyo technical institute and Bacillus subtilis NB22 is respectively to Rhizoctonia solani, the tomato disease that Fusarium oxysporum and Pseudomonas solanacearum causes has good preventive effect (Asaka O, Shoda M.Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14 [J] .Appl Environ Microbiol, 1996,62 (11): 4081-4085.).Much excellent bacillus subtilis strain is applied to production practice.
China utilizes the applied research of subtilis controlling plant diseases also to reach advanced international standard.The B916 bacterial strain of Jiangsu Academy of Agricultural Sciences's Plant Protection Institute has remarkable restraining effect to multiple pathogenic fungi and rice leaf spot bacteria, reaches 50-81% to rice sheath blight disease field efficacy, can improve protection effect after mixing by a certain percentage with jinggangmeisu.Carry out agriculture chemical registration at present, year usable floor area reach 67000 hectares.Agricultural University Of Nanjing biocontrol microorganisms B3 reaches 50-80% to wheat hypochnus field efficacy, and volume increase reaches 5.6-20.2%.Laiyang Agricultural College BL03 and XM16 bacterial strain reach 90% to Apple Mould Core and cotton anthracnose field efficacy.B.subtilis S9 and biocontrol microorganisms viride, chaetomium can be used in combination, improve protection effect (Lin Fucheng, Li Debao. subtilis Bacillus subtilis S9 is to the bacteriolysis [J] of plant pathogenic fungi. Plant Pathology, 2003,33 (2): 174-177), developed that biological prevention and control agent product prestige is green to be reached (VIRIDA), in Zhejiang Province vegetable crop, use accumulative popularizing area to reach 333 hectares.B.subtilis S0113 has strong bacteriostatic action (woods east to rice leaf spot bacteria (Xan thomonas Oryzae pv.Orzae), Xu Qing, Liu Yizhou, Deng. the bacteriostatic action of subtilis S0113 secretory protein and the separation and purification [J] of Antagonistic protein. Journal of Agricultural Biotechnology, 2001,9 (1): 77-80.).
But, biological pesticide can produce certain restraining effect to these pathogens in soil thus plays disease-proof functions, but a large amount of microorganisms existed in soil also can affect the survival of the main component living microorganism in biological pesticide thus affect its diseases prevention usefulness, there is very strong Local Adaptation in the microbial pesticide of control soil-borne disease of crop, and different weather condition and soil property all have impact on the performance of the biological and ecological methods to prevent plant disease, pests, and erosion effect of functional microorganism.Therefore many microbial pesticides in other place with fine diseases prevention usefulness, have arrived Xinjiang and have many times showed instability.
This is just in the urgent need to utilizing the efficient disease-preventing microbial in the applicable native country filtered out from Xinjiang, the new biological pesticide used under exploitation is suitable for Xinjiang Special Geographic weather condition, not only can control based on the generation of the soil-borne disease on the cash crop of processing tomato and cotton well, also can ensure Agriculture in Xinjiang and sustainable economic development, good economy and ecological benefits will be obtained simultaneously.
Summary of the invention
The object of this invention is to provide a kind of be suitable for Xinjiang Special Geographic climatope under can control the cash crop based on processing tomato well disease prevention growth-promoting bacterial strain and uses thereof of occurring of damping-off disease.
DPPG-26 bacterium of the present invention is subtilis, preservation date: on June 30th, 2014, depositary institution's title: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preserving number: CGMCC No.9393, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.Its feature is that this bacterium is gram-positive microorganism, this bacterium can produce Antagonistic protein and chitinase, there is the anti-adversity ability that broad-spectrum antibacterial is active and extremely strong, and surely can grow in the procreation of fungal pathogen mycelium surface, effective suppression pathogen growth, can the generation of effectively preventing processing tomato seedling blight in early spring evil, provide technical support for improving the quality and yield of processing tomato.
Particular content is as follows:
In the present invention, first from the particular surroundings of Xinjiang, separation screening obtains the biocontrol microorganisms DPPG-26 bacterial strain suppressing rhizoctonia solani growth.Identify through physiological and biochemical test: bacterial strain DPPG-26 is microscopy after gramstaining and spore staining, found that, this bacterium can see that after spore staining the thalline of red rod-short is middle green gemma, shape approximation is oval, all in bluish voilet after gramstaining, KOH experiment does not have filament to pull out, and is gram-positive microorganism.The 16S rDNA qualification of bacterial strain shows, the 16S rDNA sequence fragment size of the bacterial strain DPPG-26 that gene sequencing obtains is 1546bp, sequence homology compare of analysis is carried out in NCBI website, in conjunction with the qualification result of physiological and biochemical property, DPPG-26 is subtilis (Bacillus subtilis).
Be inoculated into by the DPPG-26 of activation in BPY substratum, initial pH value of medium is 6.0, and liquid amount is the bottled liquid 50mL of 250mL triangle, and inoculum size is 5%, in the shaking table of temperature 30 DEG C, cultivate 48h, and rotating speed is 180r/min, and obtaining viable count is 10
9--10
11cfu/mL.
Adopt zymocyte liquid of the present invention to soak seed after Processing tomato seed and bacterium liquid irrigating root inoculation process in seedling stage, obviously can increase the percentage of germination of Processing tomato seed, the fresh weight of seedling rate and seedling and dry weight, further illustrate this bacterial strain and there is certain disease prevention growth-promoting effect.
Concrete technical measures are by the fermented liquid (10 of DPPG-26 bacterial strain
9--10
11cfu/mL) soak Processing tomato seed and bacterium liquid irrigating root inoculation process 1 time in seedling stage, and establish 3 to contrast (CK
1: sterile medium is soaked seed, soil inoculation rhizoctonia; CK
2: sterile medium is soaked seed, and soil is sterilized soil; CK
3: dress seed with the chemical pesticide PCNB of seed weight 0.8%, soil inoculation rhizoctonia).Investigation seedling rate and incidence.Adopt zymocyte liquid root irrigation Processing tomato seed of the present invention, have good prevention effect to processing tomato seedling blight in early spring, its prevention effect is 58.62%, and the prevention effect of PCNB is 12.62%.The dry weight of each process and fresh weight are measured, result shows that its its plant plant height, fresh weight and dry weight of processing processed of zymocyte liquid of the present invention adds 23.81%, 42.57% and 30.38% respectively, effectively can increase the nourishing body biomass of plant.
By carrying out grade ammonium sulfate salting-out to tunning of the present invention, ninhydrin reaction shows the antimicrobial substance mainly creating protein in the process that zymophyte of the present invention grows.This Antagonistic protein is except having higher bacteriostatic activity to processing tomato rhizoctonia solani, also there is good restraining effect to eggplant sclerotium, tomato early blight bacterium, withered germ of water-melon, tomato wilt bacterium, also have certain fungistatic effect to pathogenetic bacteria Acidovorax Avenae Subsp.
Described diseases prevention bacterial strain is bacillus subtilis, and strain fermentation stoste inhibitory potency is 213.3U
i, this bacterium can produce chitinase, can be good at the extension suppressing pathogenic bacteria Rhizoctonia Solani, can the generation of effectively preventing processing tomato damping-off.
Beneficial effect of the present invention is as follows:
The invention provides a kind of disease prevention growth-promoting bacterial strain DPPG-26 (CGMCC No.9393) being suitable for Xinjiang Special Geographic weather condition application, diseases prevention ability is strong, effect is stablized.Particularly can control the biocontrol strain of the dry thread Pyrenomycetes morbidity of the cash crop based on processing tomato well, there is the novel biopesticide used under stronger potential development is suitable for Xinjiang Special Geographic weather condition.
Accompanying drawing explanation
Fig. 1: the impact that different culture media grows DPPG-26
The DEAE-sepharose ion-exchange chromatography figure of Fig. 2: DPPG-26 antibacterial crude protein
Fig. 3: chitinase flat board face-off experiment after purifying
Fig. 4: the antibacterial microscopic examination of chitinase after purifying
Embodiment
Be described in further detail invention below by specific embodiment, following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.
The qualification of embodiment 1 disease prevention growth-promoting bacterium DPPG-26
Be inoculated into by the DPPG-26 of activation in BPY substratum, initial pH value of medium is 6.0, and liquid amount is the bottled liquid 50mL of 250mL triangle, and inoculum size is 5%, in the shaking table of temperature 30 DEG C, cultivate 48h, and rotating speed is 180r/min.
According to morphological features, colonial morphology and gramstaining result, carry out the qualification of physio-biochemical characteristics in conjunction with " uncle outstanding Bacteria Identification handbook " (Bergey ' s Manual of etermitive Bacteriology) the 8th edition and " common bacteria identification handbook ".And the 16S rDNA of genus bacillus identifies.
Identify through physiological and biochemical test: bacterial strain DPPG-26 is that the thalline of red rod-short is middle green gemma, and shape approximation is oval, all in bluish voilet after gramstaining, KOH experiment does not have filament to pull out, and is gram-positive microorganism.
The physio-biochemical characteristics of table 1 bacterial strain DPPG-26
Note: "+": react and be positive or can grow, utilize; "-": react and be negative or cannot grow, utilize.
16S rDNA identifies
The 16S rDNA sequence fragment size of the DPPG-26 that order-checking obtains is 1546bp, bacterial strain DPPG-26 strain morphology, physiological and biochemical property meet bacillus subtilis Pseudomonas (Bacillus subtilis) feature, and sequential analysis shows, the homology of bacterial strain DPPG-26 and Bacillus atrophaeus strain ATCC 51189 (EF188847.1) is the highest, and homology is more than 99%.Identify thus, bacterial strain is subtilis (Bacillus subtilis)
The cultural characters of embodiment 2 subtilis DPPG-26
1, BPY substratum:
Peptone 10.0g, yeast extract 5.0g, beef extract 5.0g, NaCl 5.0g, glucose 5.0g, distilled water 1000mL, pH6.0
2, synthetic medium:
Synthetic medium: MgSO
4h
2o 0.2%, K
2hPO
41.05%, KH
2pO
40.45%, (NH
4)
2sO
41%, Trisodium Citrate 2H
2o 0.5%, 1% tobacco brown spot pathogen 20%.
As can be seen from Figure 1, use synthetic medium compared with BPY substratum, bacterial strain DPPG-26 biomass reduces, and during fermentation, lag phase is obviously elongated, reaches logarithmic phase at 8 hours; Reach the time advance of stationary phase, than shift to an earlier date in BPY culture medium culturing 3 little time, and there is obvious stationary phase.
Embodiment 3 subtilis DPPG-26 is to the mensuration of tomato biological amount
By the fermented liquid (10 of DPPG-26 bacterial strain
11cfu/mL) dry for subsequent use after soaking Processing tomato seed 12h.Dry thread Pyrenomycetes with wheat bran medium cultivate 5-7 days with sterilized soil with 1: 8 volume ratio fully mix thoroughly in rear loading nutrition pot.Evenly being sown into by the Processing tomato seed processed is mixed with in the nutrition pot of dry thread Pyrenomycetes, every alms bowl 15 seeds, often processes repetition 3 times, and establishes 3 to contrast (CK
1: sterile medium is soaked seed, soil inoculation rhizoctonia; CK
2: sterile medium is soaked seed, and soil is sterilized soil; CK
3: dress seed with the chemical pesticide PCNB of seed weight 0.8%, soil inoculation rhizoctonia).Regularly water, latter 15 days investigation seedling rates and incidence of emerging, to the dry weight of each process and fresh weight through having gone mensuration.
Table 2 tomato seedling damping-off control effect testing
Table 2 result can be found out, DPPG-26 can improve the percentage of germination of tomato seeds, promotes the growth in processing tomato seedling stage, significantly can suppress pathogenic bacteria again, reduces the sickness rate of tomato seedling dry thread Pyrenomycetes, has growth-promoting and diseases prevention dual-use function.The relative prevention effect of DPPG-26 process is 58.62%, higher than the prevention effect of chemical pesticide PCNB.Its plant plant height, fresh weight and dry weight processed of zymocyte liquid adds 23.81%, 42.57% and 30.38% respectively, shows that DPPG-26 effectively can increase the nourishing body biomass of plant.
The analysis of the antibacterial crude protein of embodiment 4 subtilis PPG-26
By the antibacterial crude protein of DPPG-26 fermentation liquor grade ammonium sulfate salting-out, after centrifuging, level pad (sodium phosphate buffer of 20mmol/L pH7.0) is used to dilute respectively, after DEAE-Sepharose Fast Flow ion exchange chromatography, stepwise elution is carried out respectively with containing 0.1mol/L, 0.2mol/L, 0.3mol/L, 0.4mol/L, 0.5mol/L, 0.7mol/L and 1mol/L NaCl, and coordinate Ultraviolet Detector to detect under the wavelength of 280nm, and its bacteriostatic activity of Protein Detection eluted under collecting each eluate concentration.
As can be seen from Figure 2, DPPG-26 has four albumen elution peaks, be respectively protein concn high, without bacteriostatic activity, the stream being mainly the phosphoric acid buffer wash-out of heteroproteins wears peak, and containing 0.3mol/L, 0.4mol/L and 0.5mol/L NaCl solution wash-out activated protein peak with bacteriostatic activity out.
Embodiment 5 subtilis DPPG-26 produces chitinase anti-microbial activity
Dull and stereotyped face-off method detects anti-microbial activity
The chitinase of separation and purification is measured its bacteriostatic activity according to flat board face-off method, can find that from Fig. 3 DPPG-26 produces the extension that chitinase can be good at suppressing pathogenic bacteria Rhizoctonia Solani, inhibition zone is there is around hole, mycelia turns to be yellow near inhibition zone edge mycelia, and atrophy is curling irregular, illustrate that newborn mycelia cannot extend, have novel substance constantly to deposit, bacterial strain DPPG-26 produces the synthesis that chitinase effectively can suppress the newborn mycelia of pathogenic bacteria, has bacteriostatic action.
The optical micro analysis of Antibacterial Mechanism
Produce the effect of the ripe mycelia of chitinase Rhizoctonia solani for detecting DPPG-26, after the chitinase process Rhizoctonia Solani 48h of purifying, (20 × 20 times) observations under an optical microscope, to be treated to contrast with tri-distilled water.
Can obviously find out from Fig. 4, use the pathogenic bacteria mycelia fracture of chitinase process, thin sporoderm is degraded, and protoplasma leaks, and illustrates that DPPG-26 produces chitinase and can kill miliary damping-off pathogenic fungi, has germicidal action.
Claims (8)
1. a strain prevents and treats subtilis (Bacillus subtilis) the DPPG-26 bacterial strain of damping-off, this bacterial strain is in depositary institution's preservation that State Intellectual Property Office specifies, preservation date is on June 30th, 2014, depositary institution's title: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preserving number: CGMCC No.9393.
2. bacterial strain described in claim 1, is characterized in that described subtilis DPPG-26 is obtained by separation screening in the particular surroundings of Xinjiang, on BPY substratum, cultivates 48h under 30 DEG C of conditions.Substratum is creamy white, approximate ellipsoidal, central protrusion, unstressed configuration.
3. bacterial strain according to claim 2, is characterized in that described BPY substratum is made up of following ingredients: peptone 10.0g, yeast extract 5.0g, beef extract 5.0g, NaCl5.0g, glucose 5.0g, distilled water 1000mL, pH7.0.
4. bacterial strain according to claim 2, is characterized in that this bacterium is gram-positive microorganism, has the anti-adversity ability that broad-spectrum antibacterial is active and extremely strong, and surely can grow in the procreation of fungal pathogen mycelium surface, effectively suppresses pathogen growth, controls disease generation.
5. bacterial strain according to claim 4, is characterized in that described target pathogenic fungi is pathogenic fungi: melon and fruit corruption mould (Pythiumaphanidermatum), miliary damping-off germ (Rhizoctonia solani), tomato wilt bacterium (Fusariumoxysporumf.sp.lycopersici), P. capsici (Phytophthora capsici), tomato early blight bacterium (Alternariasolani), hyphal cluster germ (Sclerotiniasclerotioum).
6. bacterial strain according to claim 4, is characterized in that described subtilis DPPG-26 produces chitinase, effectively can suppress the synthesis of the newborn mycelia of pathogenic bacteria, have bacteriostatic action.
7. bacterial strain according to claim 4, is characterized in that described subtilis DPPG-26, analyzes its antibacterial substance mainly protein through ammonium sulfate coupled ion displacement chromatography of saltouing step by step.
8. bacterial strain according to claim 4, it is characterized in that its plant plant height, fresh weight and dry weight processed of fermented liquid of described subtilis DPPG-26 adds 23.81%, 42.57% and 30.38% respectively, prevention effect is 58.62%, improve seedling rate and the percentage of germination of cotton seeds, and facilitate the growth of cotton seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410386872.6A CN104450551A (en) | 2014-08-03 | 2014-08-03 | Bacillus subtilis DPPG-26 for preventing and treating damping off and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410386872.6A CN104450551A (en) | 2014-08-03 | 2014-08-03 | Bacillus subtilis DPPG-26 for preventing and treating damping off and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104450551A true CN104450551A (en) | 2015-03-25 |
Family
ID=52897310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410386872.6A Pending CN104450551A (en) | 2014-08-03 | 2014-08-03 | Bacillus subtilis DPPG-26 for preventing and treating damping off and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104450551A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296381A (en) * | 2015-08-31 | 2016-02-03 | 哈尔滨师范大学 | Bacillus subtilis CYY-25 and application thereof |
| CN105746579A (en) * | 2016-02-02 | 2016-07-13 | 安建慧 | Application of bacillus subtilis HMB19198 preparation in control of rhizoctonia silani |
| CN108034620A (en) * | 2018-01-30 | 2018-05-15 | 青岛农业大学 | One plant of bacillus megaterium bacterial strain, peanut drop cadmium agent and its application |
| CN109679879A (en) * | 2019-01-25 | 2019-04-26 | 石河子大学 | A kind of bacterial strain, microbial inoculum and application |
| CN112159782A (en) * | 2020-10-28 | 2021-01-01 | 北京农学院 | Bacillus subtilis strain SH21 for producing chitinase, method for producing chitinase, microbial preparation and application |
| CN116240144A (en) * | 2023-03-21 | 2023-06-09 | 西安工程大学 | Bacillus phosphate-dissolving bacillus DPPG-26 and method for treating waste mushroom substrate by using same |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100914234B1 (en) * | 2009-02-17 | 2009-08-26 | 주식회사 세실 | Bacillus subtilis sm022m strain and method of controlling plant pathogens using the same |
| CN101601412A (en) * | 2009-07-16 | 2009-12-16 | 石河子大学 | The purposes of bacillus subtilis and the production method of this bacterial strain |
-
2014
- 2014-08-03 CN CN201410386872.6A patent/CN104450551A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100914234B1 (en) * | 2009-02-17 | 2009-08-26 | 주식회사 세실 | Bacillus subtilis sm022m strain and method of controlling plant pathogens using the same |
| CN101601412A (en) * | 2009-07-16 | 2009-12-16 | 石河子大学 | The purposes of bacillus subtilis and the production method of this bacterial strain |
Non-Patent Citations (4)
| Title |
|---|
| CHUN,L. ET AL.: "GenBank: FJ788426.1", 《NCBI》 * |
| LIU YAN ET AL.: "Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase", 《CHIN. J. CHEM. ENG.》 * |
| 刘永泉主编: "《农药新品种实用手册》", 31 May 2012, 北京：中国农业出版社 * |
| 黄曦等: "枯草芽孢杆菌在抑制植物病原菌中的研究进展", 《生物技术通报》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296381A (en) * | 2015-08-31 | 2016-02-03 | 哈尔滨师范大学 | Bacillus subtilis CYY-25 and application thereof |
| CN105296381B (en) * | 2015-08-31 | 2018-11-30 | 哈尔滨师范大学 | One bacillus subtilis CYY-25 and its application |
| CN105746579A (en) * | 2016-02-02 | 2016-07-13 | 安建慧 | Application of bacillus subtilis HMB19198 preparation in control of rhizoctonia silani |
| CN108034620A (en) * | 2018-01-30 | 2018-05-15 | 青岛农业大学 | One plant of bacillus megaterium bacterial strain, peanut drop cadmium agent and its application |
| CN108034620B (en) * | 2018-01-30 | 2019-09-03 | 青岛农业大学 | One plant of bacillus megaterium bacterial strain, peanut drop cadmium agent and its application |
| CN109679879A (en) * | 2019-01-25 | 2019-04-26 | 石河子大学 | A kind of bacterial strain, microbial inoculum and application |
| CN112159782A (en) * | 2020-10-28 | 2021-01-01 | 北京农学院 | Bacillus subtilis strain SH21 for producing chitinase, method for producing chitinase, microbial preparation and application |
| CN116240144A (en) * | 2023-03-21 | 2023-06-09 | 西安工程大学 | Bacillus phosphate-dissolving bacillus DPPG-26 and method for treating waste mushroom substrate by using same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104498386B (en) | The preparation method and application of raw Bacillus amyloliquefaciens strain SZ23 and zymotic fluid in wild jujube | |
| Shan et al. | Biocontrol of rice blast by the phenaminomethylacetic acid producer of Bacillus methylotrophicus strain BC79 | |
| KR101199931B1 (en) | Promotion of nitrogen mineralization of organic fertilizers and control of plant diseases using bacillus velezensis KRICT934 | |
| CN104164394A (en) | Antagonistic phytopathogen strain and application thereof | |
| CN104450551A (en) | Bacillus subtilis DPPG-26 for preventing and treating damping off and application thereof | |
| CN109355233B (en) | Bacillus amyloliquefaciens and application thereof | |
| CN108342330B (en) | Trichoderma longibrachiatum with broad-spectrum antibacterial performance and application thereof | |
| CN105670958A (en) | Low-temperature biocontrol bacterial strain and application thereof | |
| Vasanthakumari et al. | Biological control of anthracnose of chilli with rhizosphere and rhizoplane fungal isolates from grasses | |
| CN108148794A (en) | A kind of the bacillus subtilis DYr3.3 and preparation method and application of broad-spectrum antibacterial activity | |
| CN105462881B (en) | A kind of Paenibacillus polymyxa and its application for being used to prevent crop verticillium wilt | |
| CN110157638B (en) | Paenibacillus kribbensis YY-1 and application thereof | |
| CN104593283A (en) | Bacillus amyloliquefaciens DPPG-74 and its use in disease prevention and growth promotion | |
| CN106399129A (en) | Trichoderma harzianum strain and application thereof | |
| WO2023240910A1 (en) | Bacillus halotolerans and application thereof | |
| El-Mohamedy | Biological control of Pythium root rot of broccoli plants under greenhouse conditions | |
| CN109112069B (en) | Biocontrol endophytic fungus and application thereof | |
| CN102978130B (en) | Bacillus pumilus GBSC66 and its application | |
| CN105176885A (en) | Bacillus subtilis and application of bacillus subtilis in degrading organophosphorus pesticide | |
| CN106701635B (en) | Banana endophytic streptomycete with root-knot nematode killing capability and biological seedling culture substrate developed by banana endophytic streptomycete | |
| CN115960777B (en) | Bacillus pseudomycoides and application thereof in prevention and treatment of vegetable epidemic disease | |
| KR101756683B1 (en) | Bacillus amyloliquefaciens strain, microbial agent comprising the same and biotic pesticide comprising the same | |
| Türkölmez et al. | Clonostachys rosea Strain ST1140: An endophytic plant-growth-promoting fungus, and its potential use in seedbeds with wheat-grain substrate | |
| CN109609403B (en) | Biocontrol bacterium and application thereof in prevention and control of downy mildew of crops | |
| CN109456900B (en) | Composite biological preparation and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150325 |
|
| WD01 | Invention patent application deemed withdrawn after publication |