CN104593283A - Bacillus amyloliquefaciens DPPG-74 and its use in disease prevention and growth promotion - Google Patents
Bacillus amyloliquefaciens DPPG-74 and its use in disease prevention and growth promotion Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Abstract
The invention discloses bacillus amyloliquefaciens DPPG-74. The bacillus amyloliquefaciens DPPG-74 has an accession number of CGMCC No.9396. The bacillus amyloliquefaciens DPPG-74 is screened from rhizosphere soil in a processing tomato severe root rot zone and roots suffering from severe root rot, is gram-positive bacterium, has wide spectrum bacteriostatic activity and very strong stress resistance, can multiply and colonize on surfaces of fungal pathogenic mycelium, effectively inhibits pathogen growth, controls diseases, has high toxicity to pathogens and can effectively prevent and control Xinjiang processing tomato root rot in a seedling stage. Plants treated by the fermentation bacteria liquid has height, fresh weight and dry weight respectively improved by 50.39%, 91.98% and 40.41%, prevention and control rate is 67.74% and nutrient biomass of the plants is effectively improved. The bacillus amyloliquefaciens DPPG-74 allows simple culture conditions, can be preserved easily, is environmentally friendly and has good development and popularization application potential.
Description
Technical field
The invention belongs to agricultural biological technical field.More specifically, the present invention relates to a bacillus amyloliquefaciens strain DPPG-74 and disease prevention growth-promoting application.
Background technology
Tomato has another name called tomato, originates in South America Peru and Ecuador one band.Due to its have wide adaptability, cultivation easily, the feature such as output high and supply phase is long, its nutritious, unique flavor simultaneously, tomato-sauce, tomato juice, tomato fourth, tinned tomatoes and tomato sauce etc. can also be processed into except can eating raw, also there is the effect preventing cancer from occurring simultaneously, become important functional food.In tomato planting process, disease occurs very general, the disease that can endanger tomato production reaches nearly hundred kinds, wherein common are early blight, leaf mold, blight, root rot: bacterial speck, shot hole, Peptic Ulcers, blossom-end rot, mosaic virus, Streak Disease etc., these diseases, once seriously occur, all will cause destructive loss to tomato production.
Cause the pathogen kind of plant soil-borne diseases a lot (comprising fungi, bacterium, nematode, virus etc.), they infect plant root usually, cause the disease of crop root and even complete stool, concerning processing tomato root rot, pathogenic bacterium comprise the multiple soil inhabitants such as rotten mildew fungus, phytophthora root rot bacterium, dry thread Pyrenomycetes and sickle-like bacteria.Because these pathogenic bacterias can Overwintering And Survival in soil in a variety of forms, in soil, be difficult to radical cure once surely grow, and lack effective disease-resistant variety.The root rot adopting these pathogenic bacterias of chemical pesticide control to cause has achieved certain prevention effect, but chemical pesticide long pathogenic bacteria duration of service can develop immunity to drugs and cause prevention effect to decline, a large amount of chemical pesticide is administered to microbiological paper method in field soil meeting spoiled soil simultaneously, cause environment and food contamination, the green food advocated at present with people, the theory of green consumption is disagreed, the finished product produced due to Processing Tomato in Xinjiang are in addition mainly used in outlet, external quite strict to the detection of pesticide residue, many chemical pesticides all can not use in processing tomato planting process, for this reason, seek the major issue that prophylactico-therapeutic measures safely and effectively becomes urgently to be resolved hurrily in processing tomato planting process, biological control is adopted to become the ideal approach of of disease control undoubtedly.
Utilize beneficial microorganism controlling plant diseases to start from nineteen twenty-one, Hartely utilizes Antagonistic Fungus to prevent and treat cotton seedling samping off.Subsequently, all attempt all over the world to screen the control of beneficial microorganism for Plant diseases.The investigation and application of China's biocontrol of plant disease is started in early 1950s, has now filtered out many biocontrol strains soil-borne disease to good preventive effect, wherein with genus bacillus and pseudomonas in the majority.The Plant diseases biocontrol bacteria of current successful Application has some bacterial strains of the genus such as Agrobacterium, Bacillu, Pseudomonas.
Domestic having studies have reported that screens biocontrol microorganisms and application, calendar year 2001, horse equality people is separated to 2 strain sickle-like bacteria VL-21 and VL-22 from cotton bell inside, Huang can be effectively suppressed to wither the growth of bacterium and the generation (Ma Ping of Verticillium Dahliae Microsclerotia, Li Shezeng etc. utilize non-pathogenic sickle-like bacteria control cotton verticillium wilt [J] in cotton body. Chinese biological is prevented and treated, 2001,17 (2): 71-74).2006, Hao Shuzhan is separated from Sea Water of The Bohai Gulf, screen the bacterial strain (Hao Shuzhan that 41 strains have different antimicrobial spectrum, Wang Suying, Wang Lu. selection systems subtropics farming research [J] .2006 of tool anti-fungal activity of plant pathogenic marine endophytic fungus, 2 (1): 45-48).Wherein, Penicillium 67# bacterial strain not only has a broad antifungal spectrum, and also anti-microbial activity is extremely obvious, has good DEVELOPMENT PROSPECT.2007, the people such as Hou Xiaodan have carried out magnetic field mutagenesis to subtilis, obtain bacterial strain 3 strain botrytis cinerea being had to efficient antagonistic action, be respectively subtilis BS0.25-1, BS1.25 and BSO.10-1 (Hou Xiaodan, Tan Genjia, Liu Shufang etc. control effects research [J] of Bacillus subtilis by Magnetic Field on Postharvest Tomato Fruits Grey Mold Disease. laser biology journal, 2007,16 (5): 576-582).2007, Dong Hong waits by force people to be separated from yellow melon field soil to obtain picking up antibacterium subtilis Sh34 and bacteria-free filtrate all has well stable restraining effect (Dong Hongqiang to various plants pathogenic fungies such as cucumber didymella bryoniae, cucumber Fusarium oxysporum, cucumber sclerotium, scraping and printing unit, dry thread Pyrenomycetess, Mu Wei, Liu Feng etc. the qualification of bacterium Sh34 and [J] is measured to the antagonistic action of several plant pathogenic fungi. agricultural chemicals, 2007,46 (11): 779-780).2009; Xin Chunyan is separated to 1 strain has antagonistic action actinomyces strain No.37 to botrytis cinerea in tomato plants tissue; the antibiont mass-energy that this bacterial strain produces significantly suppresses mycelial growth and the spore germination of botrytis cinerea; 89.7% and 80.3% are reached respectively to the preventive effect of potted tomato seedling and therapeutic action; the field control effect of 10 times of diluents reaches 84.1% (Xin Chunyan; Zhang Liping; Xie Li etc. the research [J] of interior raw Antagonistic Actinomycetes control graw mold of tomato. Henan Agricultural Sciences, 2009 (2): 68-71).
The separation of external anti-bacteria, screening study comparative maturity, nineteen sixty-five, Lovrekovich utilizes the nontoxic variant of bacterium as inductor, successfully induce the disease resistance of plant, start the beginning (the Lovrekovich L utilizing nanobacteria innocuous variant to carry out biological control, Farkas G L.Induced protection against wildfire disease in tobacco leaves treated with heat-killed bacteria [J] .Nature, 1965,205:823-824).1980, Kerr radiates edaphic bacillus 84 bacterial strain successfully prevents and treats the fruit tree hat Australia disease caused by Agrobacterium tumefaciens, the biological control research of Plant diseases becomes focus (Kerr A.Biological control of crown gall through production of agrocin 84 [J] .Plant disease gradually, 1980,64:25-28).Nineteen ninety-five, while Silveria etc. find that 3 bacterial strains such as bacillus cereus, Bacillus coagulans and bacillus megaterium have the prophylaxis effect to bacterial wilt of tomato, percentage of germination (the Siveira EBD of seed can also be improved, Marianov R de LR, M icheref SJ, et al.Antagoinsm of Bacillus spp.against Pseudomonas solanacearurn and effect on tomato seedling growth [J] .Fitopatologia Brasileira, 1995,20 (4): 605-612).
Microbial pesticide can produce certain restraining effect to pathogen in soil thus play disease-proof functions, but a large amount of microorganism existed in soil and envrionment conditions also can affect the survival of functionally active microorganism thus affect its diseases prevention usefulness, there is very strong Local Adaptation in the microbial pesticide of control soil-borne disease of crop, therefore many function stems in other place with fine diseases prevention usefulness, have arrived Xinjiang and have many times showed instability.This is just in the urgent need to utilizing the efficient disease-preventing microbial in the applicable native country filtered out from Xinjiang, the new biological pesticide used under exploitation is suitable for the semiarid geographic climate of northwest drought, not only can control based on the generation of the soil-borne disease on the cash crop of processing tomato and cotton well, also can ensure Agriculture in Xinjiang and sustainable economic development, good economy and ecological benefits will be obtained simultaneously.
Summary of the invention
The object of this invention is to provide one and be suitable for northwest drought, semiarid geographic climate, the purposes of the biocontrol strain that the root-rot disease that particularly can control the cash crop based on processing tomato well occurs.
Bacterial strain DPPG-74 of the present invention is bacillus amyloliquefaciens Bacillus amyloliquefaciens, preservation date is on June 30th, 2014, depositary institution's title: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, preserving number: CGMCC No.9396.Its feature is that this bacterium is gram-positive microorganism, in white, and circular or sub-circular, surrounding projection, intermediate recess, unstressed configuration.There is the anti-adversity ability that broad-spectrum antibacterial is active and extremely strong, and surely can grow in the procreation of fungal pathogen mycelium surface, be have very high toxicity to pathogen, effectively suppress pathogen growth, control disease and occur, promote plant strain growth, improve crop yield.Can the generation of effectively preventing processing tomato root rot in seedling stage, there is wide popularizing application prospect.
Particular content is as follows:
In the present invention, first bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) DPPG-74 gathers from the serious soil of Root-rot Disease Processing Tomato, then obtains at the enterprising row filter of the substratum of the PDA adding target pathogenic fungi.Identify through physiological and biochemical test, bacterial strain DPPG-74 is microscopy after gramstaining and spore staining, found that, this bacterium can see that after spore staining the thalline of red rod-short is middle green gemma, shape approximation is oval, all in bluish voilet after gramstaining, KOH experiment does not have filament to pull out, and is gram-positive microorganism.The 16SrDNA qualification of bacterial strain shows, the 16 SrDNA sequence fragments of the bacterial strain DPPG-74 that gene sequencing obtains carry out sequence homology compare of analysis in NCBI website, in conjunction with the qualification result of physiological and biochemical property, DPPG-74 is bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens).
By the DPPG-74 of activation, with LB solution culture fermentation to 10
11cfu/mL is for subsequent use.
Adopt zymocyte liquid of the present invention to soak seed after Processing tomato seed and bacterium liquid irrigating root inoculation process in seedling stage, obviously can increase the percentage of germination of Processing tomato seed, the fresh weight of seedling rate and seedling and dry weight, further illustrate this bacterial strain and there is certain disease prevention growth-promoting effect.
With primary dcreening operation to the rotten mould fermenation raw liquid (10 having the DPPG-74 bacterial strain of better antagonistic action of melon and fruit
11cfu/mL) Processing tomato seed is soaked, if three contrast: CK
1(sterile medium is soaked seed, sick soil), CK
2(sterile medium is soaked seed, and soil is sterilized soil), CK
3(chemical pesticide PCNB, metaxanin, defend good fortune seed coat agent and fenaminosulf soak Processing tomato seed, sick soil).Investigation seedling rate, growth and incidence.Statistical study is carried out to result.The prevention effect of zymocyte liquid of the present invention is 67.74%, and the prevention effect of PCNB is 37.63%, the dry weight of each process and fresh weight are measured, result shows that its plant plant height, fresh weight and dry weight processed of zymocyte liquid of this bacterium of the present invention adds 50.39%, 91.98% and 40.41% respectively, effectively can increase the nourishing body biomass of plant.
The action effect of the rotten mould root rot induction diseases prevention of disease prevention growth-promoting bacterium control processing tomato:
The experiments such as observation pythium spp hypha form affected by disease prevention growth-promoting bacterium, the impact on Seed Vigor of Tomato, the impact on tomato seeds enzymic activity, show after deliberation, its impact on Pythium aphanidermatum mycelial growth is observed on this biocontrol microorganisms, determine it and can cause the degraded of scraping and printing unit silk and plasmic concentrated, explain disease prevention growth-promoting bacterium in plate face-off experimentation well and suppress the effect of growth of pathogenic bacteria.Demonstrating disease prevention growth-promoting bacterium can help Processing tomato seed to keep cell leakage, maintains the time lengthening of seed vitality.
Beneficial effect of the present invention is as follows:
The invention provides a kind of disease prevention growth-promoting bacterial strain DPPG-74 (CGMCC No.9396) being suitable for northwest drought, the application of semiarid Special Geographic weather condition, there is the anti-adversity ability that broad-spectrum antibacterial is active and extremely strong, and surely can grow in the procreation of fungal pathogen mycelium surface, diseases prevention ability is strong, effect is stablized.Particularly can control the biocontrol strain of the root rot morbidity of the cash crop based on processing tomato well, there is the novel biopesticide used under stronger potential development is suitable for Xinjiang Special Geographic weather condition.
Accompanying drawing explanation
Fig. 1: DPPG-74 impact (A:CK sterile medium on scraping and printing unit mycelial growth; B:DPPG-74).
Fig. 2: DPPG-74 impact on germination Processing tomato seed specific conductivity.
Embodiment
Be described in further detail invention below by specific embodiment, following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.
The selection systems of embodiment 1 disease prevention growth-promoting bacterium DPPG-74
The step of screening is as follows:
The first step: sampling
Gather from the soil that processing tomato root rot in seedling stage in early spring is serious.
Second step: enrichment
Respectively 10g is taken by adopting the soil sample come from processing tomato producing region, add in l00mL aqua sterilisa, then after adding appropriate granulated glass sphere thermal agitation 30min (fully being broken up by soil particle), leave standstill l0min, getting supernatant liquor 1mL adds in the triangular flask filling 99mL aqua sterilisa, carries out gradient dilution.Separation of bacterial gets 10
-4, 10
-6concentration.Each for above-mentioned diluent 200 μ l are added on PDA, NA and Gao Shi No. 1 culture medium flat plate respectively, uses spreader even spread, be inverted in after cooling in 28 DEG C of incubators and cultivate, in triplicate.After 3-7d, from each ware, single bacterium colony purifying on test tube slant of picking different shape is to be measured.
3rd step: primary dcreening operation
Target pathogenic bacteria is inoculated on sub-PDA culture medium flat plate, cultivate in 28 DEG C of biochemical cultivation cases.After mycelia is covered with, make diameter with punch tool is that 6mm bacterium block is stand-by.Screening adopts dull and stereotyped face-off method: at the dull and stereotyped central vaccination target pathogenic fungi of PDA, simultaneously around pathogenic bacteria apart from the equidistant inoculation strains tested in 2cm place, mycelia edge, cultivate check result after 2-4d at 25-28 DEG C, record antibacterial bandwidth.
4th step: multiple sieve
Pot experiment designs, with primary dcreening operation to the rotten mould fermenation raw liquid (10 having the bacterial strain of better antagonistic action of melon and fruit
11cfu/ml) soak Processing tomato seed, dry for subsequent use after soaking 12h.Then the Processing tomato seed handled well is evenly sown in nutrition pot, CK
1(sterile medium is soaked seed, sick soil), CK
2(sterile medium is soaked seed, and soil is sterilized soil), CK
3(chemical pesticide PCNB, metaxanin, defend good fortune seed coat agent and fenaminosulf soak Processing tomato seed, sick soil).Every alms bowl 20 seeds, often process three repetitions, regularly water: after seed sprouting, 30d investigates seedling rate and incidence.Carry out statistical study to result, the bacterial strain selecting the rear percentage of germination of process to be significantly higher than contrast carries out thalline qualification.
Table 1 Antagonistic Fungi is to the mensuration of processing tomato seedling rate and incidence
Note: the different letter of each row of each process is that P<0.05 level difference is remarkable.
Table 1 result can be found out, DPPG-74 can improve the percentage of germination of Processing tomato seed, and percentage of germination improves 48.20% significantly can suppress pathogenic bacteria again, and reduce the sickness rate of tomato seedling rotten mildew fungus, sickness rate reduces 40%, has growth-promoting and diseases prevention dual-use function.
5th step: qualification
According to morphological features, colonial morphology and gramstaining result, carry out the qualification of physio-biochemical characteristics in conjunction with " uncle outstanding Bacteria Identification handbook " (Bergey ' s Manual of etermitive Bacteriology) the 8th edition and " common bacteria identification handbook ".And the 16SrDNA qualification of genus bacillus.
Through physiological and biochemical test qualification, bacterial strain DPPG-74 is that the thalline of red rod-short is middle green gemma, and shape approximation is oval, and all in bluish voilet after gramstaining, KOH experiment does not have filament to pull out, and is gram-positive microorganism.Morphology, the physiological and biochemical property of this bacterial strain of comprehensive analysis DPPG-74, meet bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) feature.
The physio-biochemical characteristics of table 2 bacterial strain DPPG-74
" ten ": react and be positive or can grow, utilize; " one " reacts and is negative or cannot grows, utilizes.
16S rDNA identifies
The 16 S rDNA sequence fragment sizes of the DPPG-74 that order-checking obtains are 1456bp, and show through sequential analysis, the homology of bacterial strain DPPG-74 and Bacillus amyloliquefaciens 17 is the highest, and homology is more than 99%.Identify thus, bacterial strain is bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens DPPG-74)
Embodiment 2 bacillus amyloliquefaciens DPPG-74 is to processing tomato disease prevention growth-promoting characteristic
With primary dcreening operation to the rotten mould fermenation raw liquid (10 having the DPPG-74 bacterial strain of better antagonistic action of melon and fruit
11cfu/ml) soak Processing tomato seed, dry for subsequent use after soaking 12h.Then the Processing tomato seed handled well is evenly sown in nutrition pot, if three contrast: CK
1(sterile medium is soaked seed, sick soil), CK
2(sterile medium is soaked seed, and soil is sterilized soil), CK
3(chemical pesticide PCNB, metaxanin, defend good fortune seed coat agent and fenaminosulf soak Processing tomato seed, sick soil).Every alms bowl 20 seeds, often process three repetitions, regularly water: after seed sprouting, 30d investigates seedling rate, growth and incidence.Statistical study is carried out to result.
Table 3 Antagonistic Fungi measures the rotten mould root rot prevention effect of tomato
Note: the different letter of each row of each process is that P<0.05 level difference is remarkable.
Table 3 result can be found out, DPPG-74 can improve the percentage of germination of Processing tomato seed, promotes the growth in processing tomato seedling stage, significantly can suppress pathogenic bacteria again, reduces the sickness rate of tomato seedling rotten mildew fungus, has growth-promoting and diseases prevention dual-use function.The relative prevention effect of DPPG-74 is 67.74%, higher than the prevention effect of chemical pesticide PCNB, its plant plant height, fresh weight and dry weight processed of the zymocyte liquid of this bacterium adds 50.39%, 91.98% and 40.41% respectively, effectively can increase the nourishing body biomass of plant.
Embodiment 3 bacillus amyloliquefaciens DPPG-74 affects pythium spp hypha form
Be add 20mL PD nutrient solution in the sterile petri dish of 90mm at diameter, the bacterium sheet of access a slice diameter 8mm, be placed in 6 DEG C of constant temperature culture 2d, after cultured mycelia sheet bacillus amyloliquefaciens DPPG-74 fermented liquid and thalline process 24h, picking mycelia examines under a microscope hypha form, and compares with the hypha form without the process of disease prevention growth-promoting bacterium.Each process repetition four times, and establish clear water to contrast.
For examination biocontrol microorganisms DPPG-74, the preliminary study of carrying out antimicrobial mechanism will be processed to scraping and printing unit filament.As shown in Figure 1, biocontrol microorganisms can be observed under the microscope on the mycelial impact of Pythium aphanidermatum, and the Pythium aphanidermatum mycelium morphology with the process of disease prevention growth-promoting bacterium after 48h and untreated mycelium morphology have very big-difference.Rotten mildew fungus mycelia without the process of disease prevention growth-promoting bacterium is full, cell walls is smooth, but after disease prevention growth-promoting bacterium process 48h, pathogenic bacteria mycelium disintegrates, part hyphal cell is distorted, the phenomenon having protoplasma concentrated occurs, and illustrates that the mycelial growth of disease prevention growth-promoting bacterium to pathogenic bacteria creates disadvantageous effect.
Embodiment 4 bacillus amyloliquefaciens DPPG-74 is on the impact of Seed Vigor of Tomato
Choose the in the same size and Processing tomato seed had no mechanical damage, often 20 seeds are selected in process, rinse for several times, blot the moisture on surface with filter paper with distilled water.Be respectively charged in 25mL triangular flask, (concentration is l × l0 for examination biocontrol microorganisms fermented liquid to add l0mL in bottle respectively
10cfu/mL), contrast adds l0mL distilled water, in 25 DEG C of immersions.Measure the specific conductivity of soak solution when just having started to soak seed, as initial value (a
1), soak (the abundant imbibition of seed) after 8 hours, measure the specific conductivity (a of soak solution
2).Then seed and soak solution thereof are placed in 100 DEG C of water-baths and boil 30-90min, take out and be cooled to 25 DEG C, survey the specific conductivity (a boiling rear seed soak solution
3).
Relative conductivity (%)=(a of seed
2.a
1) × 100/ (a
3.a
1)
As shown in Figure 2, all low than contrast by the specific conductivity after bacillus amyloliquefaciens DPPG-74 process tomato seeds.Visible, disease prevention growth-promoting bacterium has maintenance seed cell film and stablizes and maintain the effect of vigor.
Sequence table
Claims (7)
1. a bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) DPPG-74 bacterial strain, this bacterial strain is in depositary institution's preservation that State Intellectual Property Office specifies, preservation date is on June 30th, 2014, depositary institution's title: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), preserving number: CGMCC No.9396.
2. bacterial strain described in claim 1, is characterized in that described bacillus amyloliquefaciens strain DPPG-74 gathers from the serious rhizosphere soil of Root-rot Disease Processing Tomato or plant root, then obtains at the enterprising row filter of the substratum of the PDA adding target pathogenic fungi.
3. bacterial strain described in claim 1, it is characterized in that described bacillus amyloliquefaciens strain DPPG-74 is gram-positive microorganism, optimum growth temperature is 25-30 DEG C, in white on substratum, circular or sub-circular, surrounding projection, intermediate recess, unstressed configuration.
4. bacterial strain described in claim 1, it is characterized in that described bacillus amyloliquefaciens strain has the active and extremely strong anti-adversity ability of broad-spectrum antibacterial, and pathogenic bacteria mycelium can be made to disintegrate, part hyphal cell is distorted, cause the concentrated generation of protoplasma, effectively suppress pathogen growth.
5. bacterial strain according to claim 2, is characterized in that described target pathogenic fungi is pathogenic fungi: melon and fruit corruption mould (Pythiumaphanidermatum), miliary damping-off germ (Rhizoctonia solani), tomato wilt bacterium (Fusariumoxysporumf.sp.lycopersici), P. capsici (Phytophthora capsici), tomato early blight bacterium (Alternaria solani), hyphal cluster germ (Sclerotini asclerotioum).
6. bacterial strain according to claim 2, is characterized in that described PDA substratum is made up of following ingredients: potato 200g, glucose 20g, agar 15g, distilled water 1000mL, pH6.8.
7. bacterial strain according to claim 4, it is characterized in that its plant plant height, fresh weight and dry weight processed of zymocyte liquid of described bacillus amyloliquefaciens strain DPPG-74 adds 50.39%, 91.98% and 40.41% respectively, prevention effect is 67.74%, effectively can increase the nourishing body biomass of plant.
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| CN115261282B (en) * | 2022-08-26 | 2023-06-27 | 甘肃省科学院生物研究所 | Bacillus amyloliquefaciens and application thereof in cucumber disease prevention and control |
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