CN106977344A - A kind of mulch biological organic fertilizer and preparation method thereof - Google Patents
A kind of mulch biological organic fertilizer and preparation method thereof Download PDFInfo
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- CN106977344A CN106977344A CN201611223946.XA CN201611223946A CN106977344A CN 106977344 A CN106977344 A CN 106977344A CN 201611223946 A CN201611223946 A CN 201611223946A CN 106977344 A CN106977344 A CN 106977344A
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- organic fertilizer
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention discloses a kind of mulch biological organic fertilizer and preparation method thereof, it belongs to the technical field of microbiological fermented organic fertilizer.40 parts of pleurotus eryngii mushroom bran, 20 parts of bog moss, 20 parts of chicken manure, 5 parts of bone meal, 10 parts of bean cake powder, 2 parts of composite bacteria agent No. 1,2 parts of composite bacteria agent No. 2 by weight percentage, using 2 zymotechniques.This mulch biological organic fertilizer has the ability of retain water and nutrients, and stable in physicochemical property can be used directly as crops base fertilizer, and every mu of 250 350 kilograms of use can significantly improve soil physico-chemical property, has effects that phosphorus decomposing, potassium decomposing, fixed nitrogen, raising utilization rate of fertilizer；Beneficial microorganism metabolism in organic fertilizer, producing natural phytohormone stimulates root growth, the degeneration-resistant ability of enhancing plant disease-resistant；Soil disease worm can also be reduced simultaneously, and the especially preventing and treating to crops root-knot nematodes disease has remarkable result, and nematode slip is up to 70%.
Description
Technical field
The invention discloses a kind of mulch biological organic fertilizer and preparation method thereof, it, which belongs to microbial fermentation manufacture biology, has
The technical field of machine fertilizer.
Background technology
In recent decades, with economic sustainable growth, people are to agriculture output demand more and more higher, in order to meet people
To grain, the high yield hope of vegetables, put into substantial amounts of agricultural chemicals and chemical fertilizer.Chemical fertilizer, the high investment of agricultural chemicals are caused in agricultural planting
The physical chemistry and biological property of soil is set to change, content of organic matter reduction, edaphon bacterial diversity is by broken
It is bad, soil function reduction etc. phenomena generation, crops particularly vegetable and fruit largely use agricultural chemicals, medicament residue compared with
Weight, for the crowd of residues of pesticides is eaten for a long time, ill dangerous, in order to improve the environment of soil, improves crop
Disease-resistant, pest-resistant ability, those skilled in the art invented mulch biological organic fertilizer.
Root-knot nematode is one of China's grain, vegetables, the great pathogen of industrial crops, and current root-knot nematode becomes north
The destructive disease of square vegetable at protected field, is also the Major Diseases of southern outdoor cropping fruits and vegetables.
The obligatory parasitism that root-knot nematode is parasitized in root system of plant is biological, with host range extensively, strong adaptability, breeding
The features such as ability is strong, makes than large effect and harm to modern agricultural production and development.With China's vegetable cultivation area
Increase, the generation of crop root knot nematode disease aggravates year by year, after disease occurs, and typically results in yield and reduces 20%-30%, seriously
When reach more than 75%, or even total crop failure.Counted according to related data, the crops of the whole world every year caused by root-knot nematode endangers
Economic loss is about more than 100,000,000,000 dollars, while root-knot nematode pest and disease damage has aggravated the generation of silborne fungal diseases, such as：It is withered
Disease, root rot.
The content of the invention
The problem of for soil environment deterioration and pest and disease damage, the present invention have developed a kind of mulch biological organic fertilizer, can both change
Become soil physico-chemical property, build up fertility, the generation of soil pest and disease damage can be suppressed again, there is significant preventing and treating for root knot nematode disease
Effect.
The present invention adopts the following technical scheme that, using the technique of 2 fermentations, first mixed material by weight percentage, adjusts
Moisture content is to 65%, and inoculating compound bacterium agent 1 treats that temperature rises to 45-70 DEG C, and ferment 3-5d, is inoculated with after temperature is down to 45 DEG C
Composite bacteria agent 2, maintains 30-50 DEG C of 3-5d of temperature, just obtains mulch biological organic fertilizer.
Composite bacteria agent 1
0.5-1.0 parts of Lactobacillus plantarum of 0.8-1.4 parts of bacillus subtilis
0.5-1.0 parts of trichoderma viride of 0.5-1.0 parts of aspergillus niger
0.5-1.0 parts of candida utili
Composite bacteria agent 2
0.2-0.6 parts of colloid bacillus cereus of 0.2-0.6 parts of bacillus megaterium
0.4-0.8 parts of Paecilomyces lilacinus of 0.2-0.6 parts of rihizobium japonicum
0.1-0.4 parts of Rhodopseudomonas palustris
The production method of described mulch biological organic fertilizer, by weight percentage including 40 parts of pleurotus eryngii mushroom bran, peat
20 parts of moss, 20 parts of chicken manure, 5 parts of bone meal, 10 parts of bean cake powder, 1 part of composite bacteria agent No. 1,1 part of composite bacteria agent No. 2.
The production method of described mulch biological organic fertilizer, the preparation method that composite bacteria agent is No. 1, No. 2 is as follows：
(1) it is inoculated with：Each strain is inoculated on slant medium respectively, bacillus subtilis, bacillus megaterium,
Colloid bacillus beef-protein medium, 30-37 DEG C of culture 2-4d；Rihizobium japonicum mannitol yeast juice culture medium
25-30 DEG C of culture 3-6d；Lactobacillus plantarum lactic acid bacteria culturing medium, 25-30 DEG C of culture 3-6d；Aspergillus niger, Trichoderma viride Cha Shi trainings
Support base, 25-28 DEG C of culture 5-7d；Candida utili, 28-32 DEG C of Paecilomyces lilacinus PDA culture medium, cultivate 3-6d；Marsh is red
Pseudomonad cultivation temperature is 30-35 DEG C, incubation time 3-6d.
(2) first class inoculum culture：The strain is inoculated in 50ml fluid nutrient mediums respectively, is put into shaking table and vibrates,
Temperature is 25-37 DEG C, shakes 2-5d, total plate count is reached 100 × 108CFU/ml。
(3) second order fermentation：First class inoculum is inoculated in 500ml fluid nutrient mediums respectively by 2-5% inoculum concentration, temperature
For 25-37 DEG C, 2-5d is shaken, total plate count is reached 20 × 108CFU/ml。
(4) three grade fermemtation：Mixed bacterium solution is inoculated in the fermentation tank for filling fluid nutrient medium, inoculum concentration 2%-
5%, 25-37 DEG C of culture 4-7d, total plate count 20 × 10 of fermenting8CFU/ml.Mixing by mass percentage is composite bacteria agent 1
Number, No. 2.
The production method of described mulch biological organic fertilizer, the composition of fluid nutrient medium：Starch 40g, brown sugar 80g, albumen
Peptone 4g, (NH4)2SO40.24g, K2HPO40.8g, MnSO40.1g, MgSO4·7H2O 0.2g, water 1000ml, pH6.0-8.0.
The composition of the present invention is further illustrated with embodiment below and is used.
Embodiment 1
40 parts of pleurotus eryngii mushroom bran, 20 parts of bog moss, 20 parts of chicken manure, 5 parts of bone meal, 10 parts of bean cake powder are mixed by weight percentage
Mixed material, regulation moisture to 65%, inoculating compound bacterium agent 1 treats that temperature rises to 45-70 DEG C, and ferment 4d, treats that temperature is down to 45
Inoculating compound bacterium agent 2 after DEG C, maintain 30-50 DEG C of 5d of temperature, just obtain mulch biological organic fertilizer.
Wherein, composite bacteria agent 1, the preparation method of No. 2 are：
(1) it is inoculated with：Each strain is inoculated on slant medium respectively, bacillus subtilis, bacillus megaterium,
Colloid bacillus beef-protein medium, 30-37 DEG C of culture 2-4d；Rihizobium japonicum mannitol yeast juice culture medium
25-30 DEG C of culture 3-6d；Lactobacillus plantarum lactic acid bacteria culturing medium, 25-30 DEG C of culture 3-6d；Aspergillus niger, Trichoderma viride Cha Shi trainings
Support base, 25-28 DEG C of culture 5-7d；Candida utili, 28-32 DEG C of Paecilomyces lilacinus PDA culture medium, cultivate 3-6d；Marsh is red
Pseudomonad cultivation temperature is 30-35 DEG C, incubation time 3-6d.
(2) first class inoculum culture：The strain is inoculated in 50ml fluid nutrient mediums respectively, is put into shaking table and vibrates,
Temperature is 25-37 DEG C, shakes 2-5d, total plate count is reached 100 × 108CFU/ml。
(3) second order fermentation：First class inoculum is inoculated in 500ml fluid nutrient mediums respectively by 2-5% inoculum concentration, temperature
For 25-37 DEG C, 2-5d is shaken, total plate count is reached 20 × 108CFU/ml。
(4) three grade fermemtation：Mixed bacterium solution is inoculated in the fermentation tank for filling fluid nutrient medium, inoculum concentration 2%-
5%, 25-37 DEG C of culture 4-7d, total plate count 20 × 10 of fermenting8CFU/ml.Mixing by mass percentage is composite bacteria agent 1
Number, No. 2.
The fluid nutrient medium used in incubation is：Starch 40g, brown sugar 80g, peptone 4g, (NH4)2SO40.24g,
K2HPO40.8g, MnSO40.1g, MgSO4·7H2O 0.2g, water 1000ml, pH6.0-8.0.
The mulch biological organic fertilizer main nutrient composition and its content made according to embodiment：N+P2O5+K2O >=6%, N >=
2%, P2O5>=2%, K2O >=2%, organic matter >=55%, the CFU/g of living bacteria count >=600,000,000.
The facilities vegetable greenhouse of 8 years is used in Zaozhuang City of Shandong Province Taierzhuang District kalimeris town, treatment region and check plot are set,
Treatment region is the mulch biological organic fertilizer made according to embodiment 1, and check plot is common biological organic fertilizer, according to every mu of 250-
350kg amount is applied, and as a result see the table below
The cucumber test result of table 1
Result of the test shows, is decreased obviously using the mulch biological organic fertilizer cucumber nematode disease index of embodiment 1, preventing and treating
Effect is up to 71.4%, and yield increases by 23.2%.
The tomato test result of table 2
Result of the test shows, is decreased obviously using the mulch biological organic fertilizer nematode disease index of embodiment 1, prevention effect
Up to 72.1%, yield increase by 22.0%.
The capsicum result of the test of table 3
Result of the test shows, is decreased obviously using the mulch biological organic fertilizer cucumber nematode disease index of embodiment 1, preventing and treating
Effect is up to 71.4%, and yield increases by 31.0%
This mulch biological organic fertilizer, technique is simple, and raw material is sufficient, and character is stable, not only improves soil with general organic fertilizer
The characteristic of earth, may additionally facilitate root growth, strengthen resistance, and the prevention effect for nematode reaches more than 70%.
Finally it should be noted that：Invention preferred embodiment is the foregoing is only, is not intended to limit the invention, for ability
For the technical staff in domain, it can still modify to the technical scheme described in previous embodiment, or to its middle part
Technical characteristic is divided to carry out equivalent substitution.Within the spirit and principles of the invention, any modification for being made, equivalent substitution, change
Enter, should be included in the scope of the protection.
Claims (6)
1. a kind of production method of mulch biological organic fertilizer, it is characterised in that using the technique of 2 fermentations, percentage by weight first
Than mixed material, regulation moisture to 65%, inoculating compound bacterium agent 1 treats that temperature rises to 45-70 DEG C, and ferment 3-5d, treats that temperature drops
Inoculating compound bacterium agent 2 after to 45 DEG C, maintains 30-50 DEG C of 3-5d of temperature, just obtains mulch biological organic fertilizer.
2. the production method of mulch biological organic fertilizer according to claim 1, it is characterised in that each material percentage by weight
Than meter include 40 parts of pleurotus eryngii mushroom bran, 20 parts of bog moss, 20 parts of chicken manure, 5 parts of bone meal, 10 parts of bean cake powder, 2 parts of composite bacteria agent No. 1,
2 parts of composite bacteria agent No. 2.
3. the production method of mulch biological organic fertilizer according to claim 1, it is characterised in that the composite bacteria agent 1 by
Following composition composition：
0.5-1.0 parts of Lactobacillus plantarum of 0.8-1.4 parts of bacillus subtilis
0.5-1.0 parts of trichoderma viride of 0.5-1.0 parts of aspergillus niger
0.5-1.0 parts of candida utili.
4. the production method of mulch biological organic fertilizer according to claim 1, it is characterised in that the composite bacteria agent 2 by
Following composition composition：
0.2-0.6 parts of colloid bacillus cereus of 0.2-0.6 parts of bacillus megaterium
0.4-0.8 parts of Paecilomyces lilacinus of 0.2-0.6 parts of rihizobium japonicum
0.1-0.4 parts of Rhodopseudomonas palustris.
5. the production method of mulch biological organic fertilizer according to claim 1, it is characterised in that composite bacteria agent No. 1, No. 2
Preparation method is as follows：
(1) it is inoculated with：Each strain is inoculated on slant medium respectively, bacillus subtilis, bacillus megaterium, colloid
Bacillus beef-protein medium, 30-37 DEG C of culture 2-4d；Rihizobium japonicum mannitol yeast juice culture medium 25-30
DEG C culture 3-6d；Lactobacillus plantarum lactic acid bacteria culturing medium, 25-30 DEG C of culture 3-6d；Aspergillus niger, Trichoderma viride czapek's medium,
25-30 DEG C of culture 5-7d；Candida utili, 28-32 DEG C of Paecilomyces lilacinus PDA culture medium, cultivate 3-6d；The red false unit cell in marsh
Bacterium cultivation temperature is 30-35 DEG C, incubation time 3-6d.
(2) first class inoculum culture：The strain is inoculated in 50ml fluid nutrient mediums respectively, is put into shaking table and vibrates, temperature
For 25-37 DEG C, 2-5d is shaken, total plate count is reached 100 × 108CFU/ml。
(3) second order fermentation：First class inoculum is inoculated in 500ml fluid nutrient mediums respectively by 2-5% inoculum concentration, temperature is
25-37 DEG C, 2-5d is shaken, total plate count is reached 20 × 108CFU/ml。
(4) three grade fermemtation：Mixed bacterium solution is inoculated in the fermentation tank for filling fluid nutrient medium, inoculum concentration 2%-5%,
25-37 DEG C of culture 4-7d, total plate count 20 × 10 of fermenting8CFU/ml.Mixing by mass percentage is composite bacteria agent.
6. the production method of mulch biological organic fertilizer according to claim 5, it is characterised in that fluid nutrient medium：Starch
40g, brown sugar 80g, peptone 4g, (NH4)2SO40.24g, K2HPO40.8g, MnSO40.1g, MgSO4·7H2O 0.2g, water
1000ml, pH6.0-8.0.
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| CN201611223946.XA CN106977344A (en) | 2016-12-26 | 2016-12-26 | A kind of mulch biological organic fertilizer and preparation method thereof |
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| CN201611223946.XA CN106977344A (en) | 2016-12-26 | 2016-12-26 | A kind of mulch biological organic fertilizer and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107827504A (en) * | 2017-12-18 | 2018-03-23 | 寿光菌禾生物科技有限公司 | A kind of biological organic fertilizer with prevention and control root knot nematode disease function and preparation method thereof |
| CN108239617A (en) * | 2018-02-10 | 2018-07-03 | 厦门昶青生物科技有限公司 | A kind of Paecilomyces lilacinus composite bacteria liquid and preparation method thereof |
| CN109279989A (en) * | 2018-11-16 | 2019-01-29 | 梅州市华智星技术服务有限公司 | A kind of biological organic fertilizer production technology based on mushroom slag |
| CN110054535A (en) * | 2019-04-25 | 2019-07-26 | 江南大学 | A kind of method that cooperative fermentation needle mushroom waste bacterial slag prepares bio-feritlizer |
| CN111001656A (en) * | 2019-12-26 | 2020-04-14 | 湖南素里养农业科技有限公司 | Soil cultivation method for reducing pesticide residue |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101165016A (en) * | 2007-10-12 | 2008-04-23 | 马玉新 | Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof |
| CN104761308A (en) * | 2014-12-04 | 2015-07-08 | 安徽汇丰园生物科技有限公司 | Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer |
-
2016
- 2016-12-26 CN CN201611223946.XA patent/CN106977344A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101165016A (en) * | 2007-10-12 | 2008-04-23 | 马玉新 | Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof |
| CN104761308A (en) * | 2014-12-04 | 2015-07-08 | 安徽汇丰园生物科技有限公司 | Preparation and application of soil ecosystem nursing microorganism bacterial fertilizer |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107827504A (en) * | 2017-12-18 | 2018-03-23 | 寿光菌禾生物科技有限公司 | A kind of biological organic fertilizer with prevention and control root knot nematode disease function and preparation method thereof |
| CN108239617A (en) * | 2018-02-10 | 2018-07-03 | 厦门昶青生物科技有限公司 | A kind of Paecilomyces lilacinus composite bacteria liquid and preparation method thereof |
| CN109279989A (en) * | 2018-11-16 | 2019-01-29 | 梅州市华智星技术服务有限公司 | A kind of biological organic fertilizer production technology based on mushroom slag |
| CN110054535A (en) * | 2019-04-25 | 2019-07-26 | 江南大学 | A kind of method that cooperative fermentation needle mushroom waste bacterial slag prepares bio-feritlizer |
| CN111001656A (en) * | 2019-12-26 | 2020-04-14 | 湖南素里养农业科技有限公司 | Soil cultivation method for reducing pesticide residue |
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Application publication date: 20170725 |